Melanoma in horses is a type of skin tumor commonly observed in gray horses, although it can occur in horses of any color. These tumors are typically found in areas such as the under-tail region, perineum, and around the lips and eyes. While many melanomas remain benign, some can become malignant and metastasize to other parts of the body. The underlying causes of melanoma in horses are not fully understood, but genetic factors and coat color are known to influence their development. Diagnosis often involves clinical examination and, in some cases, biopsy or imaging techniques. This page compiles peer-reviewed research studies and scholarly articles that explore the prevalence, diagnosis, progression, and management of melanoma in equine populations.
Kirker-Head CA, Loeffler D, Held JP.Malignant melanoma in a 21-year-old, gray, Arabian gelding was manifested by rapidly deteriorating lameness of the right pelvic limb. A melanotic, cutaneous mass of small dimensions was identified in the left jugular furrow. Exploratory laparoscopy revealed widespread infiltration of melanotic masses into the structures of the abdominal cavity. Necropsy indicated the lameness to have resulted from infiltration of neoplastic cells into the sacral nerves, dorsal root ganglia, proximal ischiatic nerve, and gluteal muscle fibers. The primary tumor could not be identified.
Cavatorta P, Crippa PR, Ito AS, Casali E, Ferrari MB, Masotti L.In the present paper we report a comparative study of physical properties and biochemical composition of isolated melanosomal membranes extracted from bovine eyes and from an equine spleen melanoma. Some biophysical characteristics of such membranes were obtained by steady-state and time resolved fluorescence spectroscopy using DPH as fluorescent probe. By these methods we have measured both static fluorescence polarization and fluorescence lifetimes and from the experimental data we have calculated the rotational correlation times by Perrin's equation. Since dynamic and static parameters, suc...
Baba AI, Gaboreanu M, Rotaru O, Kwieczinsky R.Malignant melanomas in 5 horses, 1 calf, 1 cow and 2 pigs were described. The disease, in white or gray horses, was tegumentally located and generalized in the organs of the abdominal cavity. Microscopically, dendritic and fusiform pigment cells and multinucleated cells were identified. In the hypophysis there microcysts and in one case a hypophyseal adenoma were encountered. Electronmicroscopically, differentiated melanophores and melanophages were also found. The mature melanosomes were intensely black and the elementary granules concentrically and lamellarly arranged. In cattle, the general...
Bastianello SS.A survey was carried out on the neoplasms of horses, donkeys and mules which are recorded in the registration files of the Section of Pathology of the Veterinary Research Institute, Onderstepoort, in the Republic of South Africa, over a 40-year period from 1935 to 1974. A total of 378 tumours are recorded, 339 of which were in horses, 32 in mules and 7 in donkeys. Sarcoids (38%), squamous cell carcinomas (23,5%), fibromas (8,2%), melanomas (8,0%), papillomas (4,5%), fibrosarcomas (3,4%) and lymphosarcomas (3,0%) accounted for 88,6% of the total. Of the 58 sarcoids for which the site or origin ...
Levene A.In man the epidermis is the final destination for most of the melanocytes which are of neural crest origin, and they migrate to a variety of sites. Dermal melanocytic distribution, conspicuous in some lower animals, has a very restricted normal distribution in man, and of the variety of anomalies which exist the blue naevus is the most frequently encountered. It is comparable to the common melanocytoma of dog and hamster. More widespread dermal melanocytoses are rare, and a unique case in which death from melanoma supervened, recently recorded by the author, is an example of a syndrome the onl...
Cockerell GL, MacCoy DM.This report provides a general overview of the pathobiology of neoplasia, and an update on the clinicopathological manifestations of lymphosarcoma, mastocytoma, histiocytoma, melanoma, sarcoid and circumanal gland tumors in domestic animals. Neoplasia represents a continuum of events from reversible hyperplasia to irreversible and pathological changes in tissue growth patterns. In some instances the causes of this disease process have been identified, but the etiology of the majority of naturally occurring neoplasms remain unknown. Surgical excision is the preferred treatment for tumors, but i...
Gebhart W, Niebauer GW.The morphological substrates of pigmented and depigmented skin as well as the structural characteristics of spontaneously developing melanomas were revealed by clinical, light- and electron microscopic methods in gray horses (Lipizzaner breed) from the Vienna Spanish Riding School. On clinical investigations in a group of 31 older horses (more than 10 years old) 20 exhibited melanomas, whereas 23 younger animals (less than 10 years of age) had no evidence for visuable melanotic tumors. Concomitantly with the progressive graying of the hair a depigmentation of the skin was frequently observed. ...
Sundberg JP, Burnstein T, Page EH, Kirkham WW, Robinson FR.In a retrospective study of neoplasms in Equidae pre;ented to the Animal Disease Diagnostic Laboratory, Purdue University, from Jan 1, 1970, to Dec 31, 1974, data were compiled on numbers and anatomic sites of neoplasms as well as on age, sex, and breed of subjects from which the neoplasms were taken. During this 5-year period, 21 neoplasms were diagnosed from 687 equine necropsies (3.1%) and 215 from 635 biopsies (33.9%), totaling 236 neoplasms from 1,322 cases (17.9%). The most common neoplasms were sarcoids (43.6%) and squamous cell carcinomas (24.6%). Papillomas (5.5%), nerve sheath tumors...
Dorn CR, Priester WA.Four hundred sixty-nine oral-pharyngeal malignancies diagnosed in dogs, cats, horses, and cattle and submitted to the Viterinary Medical Data Program between March 1, 1964, and Dec 31, 1974, were analyzed. Of these cases, 84% were in dogs. The most frequent oral-pharyngeal cancer in dogs was melanoma; in cats and horses, it was squamous cell carcinoma. In dogs, the risk of developing melanoma increased more with age than did the risk of developing squamous cell carcinoma and fibrosarcoma. Male dogs had significantly greater risk of developing fibrosarcomas and melanomas than did female dogs. T...
Ramadan RO.The clinical features and pathology of an ocular melanoma in a young born-grey mare are described. The tumour, possibly arising on the cornea, invaded the eye and extra-orbital muscles. Histologically; it consisted predominantly of spindle-shaped and polygonal cells containing melanin.
Théon AP, Wilson WD, Magdesian KG, Pusterla N, Snyder JR, Galuppo LD.To determine outcome associated with cutaneous tumors treated via intratumoral chemotherapy with cisplatin and identify risk factors affecting local tumor control and complications in equidae. Methods: Retrospective case series. Methods: 573 equidae with 630 cutaneous tumors. Methods: Medical records of horses, mules, donkeys, and ponies with cutaneous tumors treated via intratumoral chemotherapy with cisplatin were analyzed. Results: 549 horses, 13 mules, 8 donkeys, and 3 ponies with 630 histologically confirmed cutaneous tumors were included. Tumors included sarcoids (n = 409), squamous cell...
Seltenhammer MH, Sundström E, Meisslitzer-Ruppitsch C, Cejka P, Kosiuk J, Neumüller J, Almeder M, Majdic O, Steinberger P, Losert UM, Stöckl J....The Grey horse phenotype, caused by a 4.6 kb duplication in Syntaxin 17, is strongly associated with high incidence of melanoma. In contrast to most human melanomas with an early onset of metastasis, the Grey horse melanomas have an extended period of benign growth, after which 50% or more eventually undergo progression and may metastasize. In efforts to define changes occurring during Grey horse melanoma progression, we established an in vitro model comprised of two cell lines, HoMel-L1 and HoMel-A1, representing a primary and a metastatic stage of the melanoma, respectively. The cell lines ...
McCauley CT, Hawkins JF, Adams SB, Fessler JF.To determine outcome of horses in which cutaneous masses were removed with a carbon dioxide laser. Methods: Retrospective study. Methods: 32 horses. Methods: Medical records of horses with 1 or more cutaneous masses treated with a carbon dioxide laser were examined. Follow-up information was obtained through telephone interviews with owners and referring veterinarians. Results: Cutaneous masses were classified as sarcoids (15 horses), neoplastic masses other than sarcoids (squamous cell carcinoma [91; fibroma [1]; and melanoma [1]), and nonneoplastic masses (6). Minimum follow-up time was 6 mo...
Murray MJ, Cavey DM, Feldman BF, Trostle SS, White NA.Sympathetic denervation in a 20-year-old, gray, Thoroughbred-Percheron gelding was manifested by cutaneous hyperthermia and sweating over the right side of the body, demarcated by a line from the withers to the elbow and extending cranially. There was cutaneous hyperthermia over the right side of the head, but other signs of Horner's syndrome (sweating, ptosis, miosis, enophthalmos) were not present. The pattern of cutaneous hyperthermia and sweating was consistent with sympathetic denervation localized to the cervicothoracic ganglion, and thoracic radiographs revealed increased density in the...
Recknagel S, Nicke M, Schusser GF.To evaluate the diagnostic value of peritoneal fluid (PF) cytology for clinical diagnosis of abdominal neoplasia in horses. Methods: Ten horses with histopathologically confirmed abdominal neoplasia, in which a PF analysis was performed, were included in this retrospective study. PF was analyzed for total protein concentration and a nucleated cell count was performed. Using cytological criteria of malignancy, the PF samples were evaluated regarding their probability of malignancy. Results: Cytologic classification of cells according to criteria of malignancy allowed a positive cytologic diagno...
de Blaauw JA, Rijkenhuizen AB, van Nieuwstadt RA, Grinwis GC, Back W.Two grey mares, a 12-year-old Arabian and a 13-year-old Lippizaner cross, were referred for chronic pelvic limb lameness and abduction of the limb during the non supporting phase of the stride. The clinical examination revealed melanomas under the tail and on the perineum, and oedema of the proximal lame limb. A mass in the pelvic area was detected on rectal palpation. Laparoscopy was performed and the provisional diagnosis of malignant melanoma was made. Histological examination of the biopsy taken under laparoscopic control from the mass in the pelvis confirmed the diagnosis of malignant mel...
Bastianello SS.A survey was carried out on the neoplasms of horses, donkeys and mules which are recorded in the registration files of the Section of Pathology of the Veterinary Research Institute, Onderstepoort, in the Republic of South Africa, over a 40-year period from 1935 to 1974. A total of 378 tumours are recorded, 339 of which were in horses, 32 in mules and 7 in donkeys. Sarcoids (38%), squamous cell carcinomas (23,5%), fibromas (8,2%), melanomas (8,0%), papillomas (4,5%), fibrosarcomas (3,4%) and lymphosarcomas (3,0%) accounted for 88,6% of the total. Of the 58 sarcoids for which the site or origin ...
Johnson PJ.Cutaneous neoplasia represents a clinical problem with which veterinarians are commonly challenged. This article addresses equine cutaneous squamous cell carcinoma, squamous cell papilloma, melanoma, mastocytosis, and lymphosarcoma. Current concepts regarding the origin, pathogenesis, diagnosis and treatment of each neoplasm is emphasized.
LeRoy BE, Knight MC, Eggleston R, Torres-Velez F, Harmon BG.A 14-year-old bay Thoroughbred gelding was presented for evaluation of a mass at the base of the tail. The mass had been present for 1 year, and recently had begun to increase in size. Additional masses were found around the eye and shoulder. A fine-needle aspirate of the tail-base mass revealed highly anaplastic round to polyhedral cells containing dark green to black cytoplasmic granules interpreted to be melanin. Histologically, the mass was composed of pleomorphic, poorly pigmented, round to polyhedral cells interpreted to be neoplastic melanocytes. With immunohistochemistry, the cells wer...
Goetz TE, Ogilvie GK, Keegan KG, Johnson PJ.Cimetidine, an H2 histamine antagonist, was used in the clinical management of progressive, multifocal melanomatosis in 3 adult gray horses. Prior to treatment, the tumors had increased rapidly in size and number in 2 horses (duration of 6 and 27 months, respectively) and slowly in the third horse (duration of 48 months). All 3 horses were treated with cimetidine (2.5 mg/kg of body weight, PO, q 8 h) for 2 months to 1 year. During treatment, the number and size of the melanomas decreased substantially (50 to 90%). The progression of the disease was halted in 2 horses and controlled in the thir...
Schaffer PA, Wobeser B, Martin LE, Dennis MM, Duncan CG.To identify epidemiological trends in cutaneous neoplasms affecting equids in central North America and compare them with previously reported trends. Methods: Retrospective case series. Methods: 3,351 cutaneous biopsy specimens from 3,272 equids with a neoplastic diagnosis. Methods: Diagnostic reports from 2 diagnostic laboratories (Colorado State University and Prairie Diagnostic Services Inc) were reviewed for frequency of specific lesions and epidemiological trends. Variables included in analyses (if known) were age, sex, breed, geographic location, date of diagnosis, location of neoplasm o...
Hewes CA, Sullins KE.To determine outcome for equids with cutaneous neoplasms treated with cisplatin-containing biodegradable beads, alone or in conjunction with debulking. Methods: Retrospective case series. Methods: 56 horses, 1 zebra, 1 donkey, and 1 mule. Methods: Medical records were reviewed. Follow-up information was obtained through telephone conversations with owners and trainers of the animals. Results: 22 tumors were sarcoids, 6 were fibrosarcomas, 1 was a fibroma, 2 were peripheral nerve sheath tumors, 11 were squamous cell carcinomas, 14 were melanomas (13 gray horses and 1 bay horse), 1 was a lymphos...
Byron CR, DeWitt MR, Latouche EL, Davalos RV, Robertson JL.Irreversible electroporation is a proven ablation modality for local ablation of soft tissue tumors in animals and humans. However, the strong muscle contractions associated with the electrical impulses (duration, 50-100 μs) requires the use of general anesthesia and, in most situations, application of neuromuscular blockade. As such, this technology is not used in an outpatient setting for ablating common cutaneous tumors (e.g., squamous cell carcinoma or melanoma) in humans or animals. Recently, high-frequency irreversible electroporation (H-FIRE) technology has been developed to enable ele...
van den Top JG, de Heer N, Klein WR, Ensink JM.Preputial and penile tumours are more common in horses than in other domestic animals, but no large surveys of male horses with tumours of the external genitalia are available. Objective: To present a retrospective analysis of male horses with neoplasms of the external genitalia. Methods: The penile and preputial tumours of 114 horses were evaluated. Data recorded included age, gelding or stallion and breed; type and site of lesion; involvement of regional lymph nodes; histopathology (including grading of squamous cell carcinoma); and results of radiographic examination of the thorax. Results:...
Cockerell GL, MacCoy DM.This report provides a general overview of the pathobiology of neoplasia, and an update on the clinicopathological manifestations of lymphosarcoma, mastocytoma, histiocytoma, melanoma, sarcoid and circumanal gland tumors in domestic animals. Neoplasia represents a continuum of events from reversible hyperplasia to irreversible and pathological changes in tissue growth patterns. In some instances the causes of this disease process have been identified, but the etiology of the majority of naturally occurring neoplasms remain unknown. Surgical excision is the preferred treatment for tumors, but i...
Giuliano EA.Neoplastic adnexal disease represents one of the most frequently encountered and therapeutically challenging ophthalmic problems of horses. This paper reviews current concepts in equine periocular neoplasia. Specifically, a literature-based review of the aetiopathogenesis of the most common tumours to affect the equine eyelid (squamous cell carcinoma, sarcoid, melanoma and lymphosarcoma) is provided. Current and emerging treatment modalities, including photodynamic therapy, are reviewed.
Cavatorta P, Crippa PR, Ito AS, Casali E, Ferrari MB, Masotti L.In the present paper we report a comparative study of physical properties and biochemical composition of isolated melanosomal membranes extracted from bovine eyes and from an equine spleen melanoma. Some biophysical characteristics of such membranes were obtained by steady-state and time resolved fluorescence spectroscopy using DPH as fluorescent probe. By these methods we have measured both static fluorescence polarization and fluorescence lifetimes and from the experimental data we have calculated the rotational correlation times by Perrin's equation. Since dynamic and static parameters, suc...
Roels S, Tilmant K, Van Daele A, Van Marck E, Ducatelle R.Melanocytic tumours are a well-known clinical and pathological entity in horses, but further phenotypic characterization of these tumours is lacking. Six melanocytic tumours from five horses (two metastatic and four benign) were examined by Ki67, PCNA and p53 immunostaining, DNA nick end labelling (Tunel) and Feulgen staining. The stainings were evaluated using quantitative image analysis. The resulting parameters of growth fraction (Ki67), S-phase index (PCNA), p53 index, apoptotic index, DNA index, nuclear diameter, ploidy balance, proliferation index (Feulgen) and hyperploidy were analysed....
Anbalagan E, Malathi S.The human body's largest organ is the skin which covers the entire body. The facial skin is one area of the body that needs careful handling. It can cause several facial skin diseases like acne, eczema, moles, melanoma, rosacea, and many other fungal infections. Diagnosing these diseases has been difficult due to challenges like the high cost of medical equipment and the lack of medical competence. However, various existing systems are utilized to detect the type of facial skin disease, but those approaches are time-consuming and inaccurate to detect the disease at early stages. To address var...
Fesmire CC, Peal B, Ruff J, Moyer E, McParland TJ, Derks K, O'Neil E, Emke C, Johnson B, Ghosh S, Petrella RA, DeWitt MR, Prange T, Fogle C, Sano MB.Integrated time nanosecond pulse irreversible electroporation (INSPIRE) is a novel tumor ablation modality that employs high voltage, alternating polarity waveforms to induce cell death in a well-defined volume while sparing the underlying tissue. This study aimed to demonstrate the in vivo efficacy of INSPIRE against spontaneous melanoma in standing, awake horses. Unassigned: A custom applicator and a pulse generation system were utilized in a pilot study to treat horses presenting with spontaneous melanoma. INSPIRE treatments were administered to 32 tumors across 6 horses and an additional 1...
Gao Y, Packeiser EM, Wendt S, Sekora A, Cavalleri JV, Pratscher B, Alammar M, Hühns M, Brenig B, Junghanss C, Nolte I, Murua Escobar H.Malignant melanomas (MMs) are the abnormal proliferation of melanocytes and are one of the lethal skin cancers in humans, equines, and canines. Accordingly, MMs in companion animals can serve as naturally occurring animal models, completing conventional cancer models. The common constitutive activation of the MAPK and PI3K pathways in MMs has been described in all three species. Targeting the related pathways is considered a potential option in comparative oncologic approaches. Herein, we present a cross-species comparative analysis exposing a set of ten melanoma cell lines (one human, three e...
Andersson L.The Grey allele in horses is causing premature hair greying and susceptibility to melanoma. The causal mutation is a 4.6 kb tandem duplication in intron 6 of the Syntaxin 17 gene. A recent study demonstrated that the most common allele at the Grey locus (G3) involves three tandem copies of this sequence, whilst a more rare allele (G2) has two tandem copies and the wild-type allele (G1) only one copy. The G3 allele is causing fast greying and high incidence of skin melanoma, whereas the G2 allele is causing slow greying and no obvious increase in melanoma incidence. Further somatic copy number ...
Pimenta J, Prada J, Pires I, Cotovio M.Equine melanomas are a common neoplasm in gray horses. However, scientific knowledge about their progression over time is quite scarce. Some owners and veterinarians still believe that early intervention is not necessary, stating that tumors evolve very slowly and intervention could worsen the animal's condition. This work aims to identify clinical and histological differences that may exist between equine melanomas with different excision intervals (time between tumor detection and surgical excision). A total of 42 tumors (13 benign and 29 malignant) from 34 horses were included in this study...
