Analyze Diet

Topic:Nutrition

Nutrition in horses encompasses the study of dietary requirements and feeding practices that support equine health, growth, and performance. It involves the analysis of nutrients such as carbohydrates, proteins, fats, vitamins, and minerals, and their roles in equine physiology. Proper nutrition is essential for maintaining optimal body condition, supporting metabolic processes, and preventing dietary-related disorders. Research in this field examines the nutritional needs of horses at different life stages and activity levels, as well as the effects of various feed types and supplements. This page compiles peer-reviewed research studies and scholarly articles that explore the composition, digestion, and impact of different dietary components on equine health and performance.
Determination of lipid profiles in serum of obese ponies before and after weight reduction by using multi-one-dimensional thin-layer chromatography.
Research in veterinary science    November 21, 2017   Volume 117 111-117 doi: 10.1016/j.rvsc.2017.11.013
Auyyuenyong R, Henze A, Ungru J, Schweigert FJ, Raila J, Vervuert I.Obesity is a key component of equine metabolic syndrome, which is highly associated with laminitis. Feed restriction and/or exercise are known to alleviate the detrimental effects of insulin resistance in obese ponies. However, little is known about changes in the serum lipid patterns due to weight reduction and its association with disease outcomes. Therefore, the lipid patterns in the serum of 14 mature ponies before and after a 14-week body weight reduction program (BWRP) were investigated by multi-one-dimensional thin-layer chromatography (MOD-TLC). Additionally, sensitivity to insulin (SI...
The oral glucose test predicts laminitis risk in ponies fed a diet high in nonstructural carbohydrates.
Domestic animal endocrinology    November 16, 2017   Volume 63 1-9 doi: 10.1016/j.domaniend.2017.10.008
Meier AD, de Laat MA, Reiche DB, Pollitt CC, Walsh DM, McGree JM, Sillence MN.The aim of this study was to investigate the relationship between laminitis development in ponies and insulin/glucose concentrations in response to the oral glucose test (OGT) and a dietary challenge high in nonstructural carbohydrates (NSCs). After undergoing an OGT (1 g dextrose/kg BW in feed), 37 ponies with 2-h serum insulin concentrations ranging from 22 to 1,133 μIU/mL were subjected to a diet challenge period (DCP), consuming 12 g NSC/kg BW/d for up to 18 d. Insulin and glucose responses were measured on day 2 of the DCP. Clinical laminitis was diagnosed by blinded experts and confi...
Evaluating the impact of domestication and captivity on the horse gut microbiome.
Scientific reports    November 14, 2017   Volume 7, Issue 1 15497 doi: 10.1038/s41598-017-15375-9
Metcalf JL, Song SJ, Morton JT, Weiss S, Seguin-Orlando A, Joly F, Feh C, Taberlet P, Coissac E, Amir A, Willerslev E, Knight R, McKenzie V, Orlando L.The mammal gut microbiome, which includes host microbes and their respective genes, is now recognized as an essential second genome that provides critical functions to the host. In humans, studies have revealed that lifestyle strongly influences the composition and diversity of the gastrointestinal microbiome. We hypothesized that these trends in humans may be paralleled in mammals subjected to anthropogenic forces such as domestication and captivity, in which diets and natural life histories are often greatly modified. We investigated fecal microbiomes of Przewalski's horse (PH; Equus ferus p...
Biochemical analysis of some serum trace elements in donkeys and horses in Eastern region of Kingdom of Saudi Arabia.
Veterinary world    October 25, 2017   Volume 10, Issue 10 1269-1274 doi: 10.14202/vetworld.2017.1269-1274
Shawaf T, Almathen F, Meligy A, El-Deeb W, Al-Bulushi S.Little is known about the serum levels of trace elements in donkeys and horses in Saudi Arabia. This study aimed to investigate the levels of some trace elements in these two species in the eastern region of Saudi Arabia and to compare the obtained results with the reference values. Methods: Seventeen Arabian horses and twenty eight Hassawi donkeys were randomly selected for this study. All of the studied healthy animals were kept under a uniform feeding protocol without any minerals supplementations. Atomic absorption spectrometer is used to estimate the serum concentrations of selenium (Se),...
A Summary of New Findings on the Biological Effects of Selenium in Selected Animal Species-A Critical Review.
International journal of molecular sciences    October 21, 2017   Volume 18, Issue 10 doi: 10.3390/ijms18102209
Hosnedlova B, Kepinska M, Skalickova S, Fernandez C, Ruttkay-Nedecky B, Malevu TD, Sochor J, Baron M, Melcova M, Zidkova J, Kizek R.Selenium is an essential trace element important for many physiological processes, especially for the functions of immune and reproductive systems, metabolism of thyroid hormones, as well as antioxidant defense. Selenium deficiency is usually manifested by an increased incidence of retention of placenta, metritis, mastitis, aborts, lowering fertility and increased susceptibility to infections. In calves, lambs and kids, the selenium deficiency demonstrates by WMD (white muscle disease), in foals and donkey foals, it is associated with incidence of WMD and yellow fat disease, and in pigs it cau...
Relationship between carcass traits, prime cuts and carcass grading from foals slaughtered at the age of 13 and 26 months and supplemented with standard and linseed-rich feed.
Animal : an international journal of animal bioscience    October 17, 2017   Volume 12, Issue 5 1084-1092 doi: 10.1017/S1751731117002555
Ruiz M, Sarriés MV, Beriain MJ, Crecente S, Domínguez R, Lorenzo JM.In order to improve foal carcass quality, it is necessary in particular to improve the carcass dressing percentage and tissue composition. Thus, it is important to establish relationships between grading systems and these parameters. This research was conducted to study the effect of slaughter age (13 v. 26 months) and finishing feed (standard v. linseed feed) on carcass characteristics such as subcutaneous fat colour plus classification of foals for the degree of fatness and conformation. For this study, 46 foals of crossbred genotype (Galician Mountain×Burguete) were used. Finishing feed di...
Influences of different dietary contents of macrominerals on the availability of trace elements in horses.
Journal of animal physiology and animal nutrition    October 13, 2017   Volume 102, Issue 2 e633-e640 doi: 10.1111/jpn.12805
Neustädter LT, Kamphues J, Ratert C.In this study, influences of a reduced macromineral intake on the trace element metabolism in horses at maintenance were investigated. Background of this study is the revised recommendation on the macromineral supply for horses (GfE ). Balance studies on three adult pony geldings with body weights of 405 / 348 / 384 kg were performed to obtain data on apparent digestibility (aD), retention and serum concentrations of different trace elements (Cu, Zn, Se) at different dietary macromineral levels. A mineral supplement or a complementary feed-with a reduced macromineral content-was added to a ha...
Rapid Communication: Dietary selenium improves skeletal muscle mitochondrial biogenesis in young equine athletes.
Journal of animal science    October 11, 2017   Volume 95, Issue 9 4078-4084 doi: 10.2527/jas2017.1919
White SH, Wohlgemuth S, Li C, Warren LK.Exercise is known to promote mitochondrial biogenesis in skeletal muscle as well as enhance mitochondrial function and efficiency in human and rodent models. These adaptations help to decrease exercise-associated production of reactive oxygen species, which can negatively affect health and performance if antioxidant mechanisms are overwhelmed. Little is known about the adaptations of mitochondria in response to exercise training in the growing horse or if supplementation with a dietary antioxidant can improve mitochondrial function. To evaluate the separate and combined effects of selenium (Se...
Incidence of laminitis and survey of dietary and management practices in pleasure horses and ponies in south-eastern Australia.
Australian veterinary journal    September 28, 2017   Volume 95, Issue 10 370-374 doi: 10.1111/avj.12635
Potter SJ, Bamford NJ, Harris PA, Bailey SR.The aims of this study were to (1) report the incidence of laminitis among a population of horses and ponies attending Pony Clubs in Victoria, Australia, and (2) describe the dietary and management practices of the sample population. Methods: Researchers visited 10 Pony Clubs over a 10-month period. Horse and pony owners completed a questionnaire to provide information on management relating to diet and exercise. Owners were also asked to report their animal's history of laminitis, if any. Results: From a survey population of 233 horses and ponies, 15.0% of animals (35 individuals) were report...
Trace elements in struvite equine enteroliths: Concentration, speciation and influence of diet.
Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS)    September 19, 2017   Volume 45 23-30 doi: 10.1016/j.jtemb.2017.09.019
Rouff AA, Lager GA, Arrue D, Jaynes J.Equine enteroliths ∼1.5cm in diameter were collected from an Arabian horse in Louisville, Kentucky, United States. Scanning electron microscopy (SEM) and light microscope imaging of a sectioned enterolith showed two distinct regions of concentric growth outward from the central nidus, a small pebble. After initial growth, acidic colonic fluids permeated the stone inducing recrystallization and alteration of crystals closest to the nidus. A second growth event, when mineral crystallization was again favorable, produced an outer region of unaltered crystals at the rim. The mineral was identifi...
Measurement of urinary concentrations of the mycotoxins zearalenone and sterigmatocystin as biomarkers of exposure in mares.
Reproduction in domestic animals = Zuchthygiene    September 18, 2017   Volume 53, Issue 1 68-73 doi: 10.1111/rda.13054
Takagi M, Uno S, Kokushi E, Sato F, Wijayagunawardane M, Fink-Gremmels J.Mycotoxins may affect animal health, including reproduction. Little is known about the clinical relevance of exposure of horses to contaminated feed. This study aimed at (i) monitoring the levels of the mycotoxins zearalenone (ZEN), with its metabolites α- and β-zearalenol (α- and β-ZOL), and sterigmatocystin (STC) in urine samples from thoroughbred mares in Japan and (ii) relating these findings to the potential effects on reproductive efficacy of breeding mares. Sixty-three urine samples of breeding mares from 59 breeding farms were used. Urine samples and reproductive records were colle...
Effect of age and dietary carbohydrate profiles on glucose and insulin dynamics in horses.
Equine veterinary journal    September 13, 2017   Volume 50, Issue 2 249-254 doi: 10.1111/evj.12745
Jacob SI, Geor RJ, Weber PSD, Harris PA, McCue ME.Glucose and insulin dynamics may be different in adult and aged horses. Objective: To determine the effects of age and dietary carbohydrates on glucose and insulin dynamics in healthy horses. Methods: Balanced Latin square with four isocaloric diets: CONTROL (hay plus restricted-starch-and-sugar fortified pellets), STARCH (control plus kibbled corn), FIBER (control plus unmolassed sugar beet pulp/soybean hull pellets) and SUGAR (control plus dextrose powder). Methods: A total of 16 healthy Thoroughbreds and Standardbreds divided into two age groups: ADULT (8.8 ± 2.9 years; n = 8) and AGE...
Effect of feed deprivation on selected parameters of lipid mobilisation and hepatic function in healthy Akhal Teke horses.
Equine veterinary journal    September 1, 2017   Volume 50, Issue 1 98-103 doi: 10.1111/evj.12730
Tóth B, Auth A, Rompos L, Bakos Z.Gastrointestinal and other systemic diseases of Equidae are often characterised by partial or complete inappetence. The catabolic processes may lead to lipid mobilisation and other metabolic disturbances. Evidence from earlier studies has revealed that during feed deprivation/inappetence, the conjugation of bilirubin is reduced causing a substantial rise of the total bilirubin and its unconjugated fraction. Objective: We hypothesised that during feed deprivation, the serum concentration of free fatty acids (FFA), triglycerides (TG), blood urea nitrogen (BUN), total bilirubin (tBIL), total bile...
Seasonal fluctuations in body weight during growth of Thoroughbred racehorses during their athletic career.
BMC veterinary research    August 18, 2017   Volume 13, Issue 1 257 doi: 10.1186/s12917-017-1184-3
Takahashi Y, Takahashi T.Domesticated horses adapt to environmental conditions through seasonal fluctuations in their metabolic rate. The seasonal change of metabolic rates of domesticated horses in pastures is documented. However, there are few investigations on seasonal body weight change of domesticated horses housed in stables, which are provided constant energy intake throughout the year. Both seasonal changes and gain in body weight of racehorses during their athletic career is known to a lesser extent because their body weight are not measured in most countries. Here, we used a seasonal-trend decomposition meth...
Equine peripheral dental caries: An epidemiological survey assessing prevalence and possible risk factors in Western Australian horses.
Equine veterinary journal    August 18, 2017   Volume 50, Issue 1 79-84 doi: 10.1111/evj.12718
Jackson K, Kelty E, Tennant M.Peripheral dental caries is defined as macroscopic destruction of the calcified dental tissues and can cause considerable dental pathology and pain. It appears to occur at a high prevalence in Western Australian horses. At present, risk factors for the condition are poorly understood, making treatment and prevention difficult. Objective: To assess the prevalence of and potential risk factors for peripheral caries in Western Australian horses. Methods: Cross-sectional, epidemiological study. Methods: A survey of 500 Western Australian horses was administered in two sections. The first section w...
Characterizing ammonia emissions from horses fed different crude protein concentrations.
Journal of animal science    August 15, 2017   Volume 95, Issue 8 3598-3608 doi: 10.2527/jas.2017.1648
Weir J, Li H, Warren LK, Macon E, Wickens C.Evaluating impact of animal agriculture on air quality has been the focus of recent research. Ammonia (NH) volatilization occurs when undigested protein in feces and urea in urine is broken down by bacteria and enzymes. Information regarding NH emission from equine facilities is limited, and effects of CP intake on NH emissions have not been investigated. Nine mature geldings were used in a 3 × 3 replicated Latin square design study to determine effects of dietary CP on potential NH losses from feces and urine. We hypothesized feeding horses above the CP requirement would result in an increas...
Changes in the Total Fecal Bacterial Population in Individual Horses Maintained on a Restricted Diet Over 6 Weeks.
Frontiers in microbiology    August 11, 2017   Volume 8 1502 doi: 10.3389/fmicb.2017.01502
Dougal K, Harris PA, Girdwood SE, Creevey CJ, Curtis GC, Barfoot CF, Argo CM, Newbold CJ.Twelve mature (aged 5-16 years) horses and ponies of mixed breed and type were fed restricted (1.25% BM Dry matter) quantities of one of two fiber based diets formulated to be iso-caloric. Diet 1 comprised of 0.8% body mass (BM) of chaff based complete feed plus 0.45% BM low energy grass hay (the same hay used for both diets). Diet 2 comprised 0.1% BM of a nutrient balancer plus 1.15% BM grass hay. Fecal samples were collected at week 10 and week 16. DNA was extracted and the V1-V2 regions of 16SrDNA were 454-pyrosequenced to investigate the bacterial microbiome of the horse. The two most abun...
Epidemiological survey on equine cheek tooth infundibular caries in the United Kingdom.
The Veterinary record    August 3, 2017   Volume 181, Issue 9 235 doi: 10.1136/vr.104319
Borkent D, Reardon R, Dixon PM.Infundibular caries (IC) is an important equine dental disorder that can cause premature wear, fractures and apical infection of affected maxillary cheek teeth. No accurate prevalence values for IC are available for UK horses. The feeding of high levels of concentrates is believed to increase its prevalence, but no objective information is available on such possible environmental risk factors. The aims of the study were to document the prevalence of IC in UK horses, assess its distribution and severity between infundibulae and teeth in affected horses and examine for potential risk factors for...
Spirulina platensis Improves Mitochondrial Function Impaired by Elevated Oxidative Stress in Adipose-Derived Mesenchymal Stromal Cells (ASCs) and Intestinal Epithelial Cells (IECs), and Enhances Insulin Sensitivity in Equine Metabolic Syndrome (EMS) Horses.
Marine drugs    August 3, 2017   Volume 15, Issue 8 237 doi: 10.3390/md15080237
Nawrocka D, Kornicka K, Śmieszek A, Marycz K.Equine Metabolic Syndrome (EMS) is a steadily growing life-threatening endocrine disorder linked to insulin resistance, oxidative stress, and systemic inflammation. Inflammatory microenvironment of adipose tissue constitutes the direct tissue milieu for various cell populations, including adipose-derived mesenchymal stromal cells (ASCs), widely considered as a potential therapeutic cell source in the course of the treatment of metabolic disorders. Moreover, elevated oxidative stress induces inflammation in intestinal epithelial cells (IECs)-the first-line cells exposed to dietary compounds. In...
Reproductive phenology of Creole horses in Ecuador in the absence of photoperiod variation: The effects of forage availability and flooding affecting body condition of mares.
Animal science journal = Nihon chikusan Gakkaiho    July 27, 2017   Volume 88, Issue 12 2063-2070 doi: 10.1111/asj.12818
Carranza J, Yoong WA, Mateos C, Caño Vergara B, Gómez CL, Macías V.Horse reproduction tends to be seasonal. The main adjusting factor in their original temperate ranges is photoperiod variation, although it is absent in equatorial areas where horses were introduced by European colonizers. Hence, dates of reproduction in these areas may be influenced by factors affecting mares' conditions and the success of foaling. Here we study reproductive timing in Creole horses in Ecuador reared in an extensive production system. We found that foaling peaked in August. Mares' conditions showed one peak in June-July, before the start of the breeding season, and another in ...
Stable isotopes reveal diet shift from pre-extinction to reintroduced Przewalski’s horses.
Scientific reports    July 20, 2017   Volume 7, Issue 1 5950 doi: 10.1038/s41598-017-05329-6
Kaczensky P, Burnik Šturm M, Sablin MV, Voigt CC, Smith S, Ganbaatar O, Balint B, Walzer C, Spasskaya NN.The Przewalski's horse (Equus ferus przewalskii), the only remaining wild horse within the equid family, is one of only a handful of species worldwide that went extinct in the wild, was saved by captive breeding, and has been successfully returned to the wild. However, concerns remain that after multiple generations in captivity the ecology of the Przewalski's horse and / or the ecological conditions in its former range have changed in a way compromising the species' long term survival. We analyzed stable isotope chronologies from tail hair of pre-extinction and reintroduced Przewalski's horse...
Effect of linseed supplementation and slaughter age on meat quality of grazing cross-bred Galician x Burguete foals.
Journal of the science of food and agriculture    July 17, 2017   Volume 98, Issue 1 266-273 doi: 10.1002/jsfa.8466
Domínguez R, Pateiro M, Crecente S, Ruiz M, Sarriés MV, Lorenzo JM.The aim of this study was to assess the effect of finishing diet (control concentrate vs. linseed concentrate) and slaughter age (13 vs. 26 months) on meat and nutritional quality of foal meat. For this study, 46 foals from crossing Galicia Mountain x Burguete breeds were used. Results: The obtained results showed that slaughter age had an influence on chemical composition and colour parameters. Foals slaughtered at the age of 13 months had lower content of intramuscular fat and higher cholesterol contents than those slaughtered at 26 months of age. Regarding colour parameters, older foals sho...
Comparative selective retention of particle size classes in the gastrointestinal tract of ponies and goats.
Journal of animal physiology and animal nutrition    July 11, 2017   Volume 102, Issue 2 429-439 doi: 10.1111/jpn.12763
Hummel J, Scheurich F, Ortmann S, Crompton LA, Gerken M, Clauss M.There is a discrepancy in the literature on potential digesta separation mechanisms in horses, with both a selective retention of fine and of large particles postulated in different publications. To assess the net effect of such mechanisms, we fed ponies on a hay-only diet a pulse dose of whole (unchopped) marked hay together with a solute marker, collected faeces on a regular basis, measured marker concentrations in whole faeces and in their large (2.0-16 mm), medium (0.5-1.0 mm) and small (0.063-0.25 mm) particle fraction, and calculated the corresponding mean retention times (MRTs). For ...
Long-term adaptation capacity of ponies: effect of season and feed restriction on blood and physiological parameters.
Animal : an international journal of animal bioscience    July 10, 2017   Volume 12, Issue 1 88-97 doi: 10.1017/S1751731117001392
Brinkmann L, Riek A, Gerken M.Domesticated horses are increasingly kept under semi-natural housing conditions, whereas their adaptation capacity is not fully investigated. In all, 10 Shetland pony mares were held under semi-extensive conditions for 1 year. In winter animals were allocated into two feeding groups (60% and 100% of maintenance energy requirement, respectively). Triiodothyronine, thyroxine, non-esterified fatty acids (NEFA), total bilirubin, total protein, triglyceride, glucose, insulin and hair length were measured at monthly intervals, whereas BW, body condition score, cresty neck score and resting heart rat...
Common lesions of the distal end of the third metacarpal/metatarsal bone in racehorse catastrophic breakdown injuries. Janes JG, Kennedy LA, Garrett KS, Engiles JB.Equine catastrophic skeletal breakdown injury is a serious issue within the racing industry, given the impact on equine and human health. The metacarpo- and metatarso-phalangeal (fetlock) joints are common sites of catastrophic injury. However, lesions involving articular cartilage, subchondral bone, and synovium are commonly identified within the fetlock of the contralateral limb; hence, it is imperative that lesions in both limbs are evaluated and characterized during postmortem examination. Bone and articular cartilage changes typically occur in specific locations, related to cyclic fetlock...
The diagnosis of equine insulin dysregulation.
Equine veterinary journal    June 23, 2017   Volume 49, Issue 5 570-576 doi: 10.1111/evj.12703
Bertin FR, de Laat MA.Insulin dysregulation is the hallmark of equine metabolic syndrome and has received attention because of its direct association with laminitis. In the absence of an adequate treatment for laminitis, a focus on prophylaxis is needed, making early detection of individuals at risk of developing laminitis one of the main challenges in equine endocrinology. Recent studies have shown that insulin dysregulation goes beyond tissue insulin resistance and it is now demonstrated that the equine enteroinsular axis plays a major role in insulin secretion and equine hyperinsulinaemia. In this review, we dis...
Interlaboratory trial for the measurement of total cobalt in equine urine and plasma by ICP-MS.
Drug testing and analysis    June 20, 2017   Volume 9, Issue 9 1400-1406 doi: 10.1002/dta.2191
Popot MA, Ho ENM, Stojiljkovic N, Bagilet F, Remy P, Maciejewski P, Loup B, Chan GHM, Hargrave S, Arthur RM, Russo C, White J, Hincks P, Pearce C....Cobalt is an essential mineral micronutrient and is regularly present in equine nutritional and feed supplements. Therefore, cobalt is naturally present at low concentrations in biological samples. The administration of cobalt chloride is considered to be blood doping and is thus prohibited. To control the misuse of cobalt, it was mandatory to establish an international threshold for cobalt in plasma and/or in urine. To achieve this goal, an international collaboration, consisting of an interlaboratory comparison between 5 laboratories for the urine study and 8 laboratories for the plasma stud...
Feed intake patterns of sport ponies and warmblood horses following iso-energetic intake of pelleted fibre-rich mixed feed, muesli feed and semicrushed oat grains.
Journal of animal physiology and animal nutrition    June 20, 2017   Volume 101 Suppl 1 37-42 doi: 10.1111/jpn.12732
Bochnia M, Boesel M, Bahrenthien L, Wensch-Dorendorf M, Zeyner A.The aim of the study was to compare diverse feed intake patterns in sport ponies and warmblood-type horses after feeding iso-energetic amounts of three different concentrates: a pelleted fibre-rich mixed feed (PF), a muesli feed (MF) and semicrushed oat grains (OG). Four sport ponies and six warmblood-type horses received the concentrates and meadow hay according to maintenance (0.52 MJ metabolizable energy (ME) kg body weight (BW) /day). Both breeds were allocated at random to three groups and received once daily for 8 days either OG (1 g starch/kg BW/meal) or iso-energetic quantities of ...
Clinical nutrition counselling service in the veterinary hospital: retrospective analysis of equine patients and nutritional considerations.
Journal of animal physiology and animal nutrition    June 20, 2017   Volume 101 Suppl 1 59-68 doi: 10.1111/jpn.12658
Vergnano D, Bergero D, Valle E.Nutrition plays a very important role in the healthy and in the ill horse. Although research in this field clearly shows that incorrect nutritional practices may lead to severe pathologies, inappropriate feeding plans often continue to be used. A clinical nutrition counselling (CNC) service could thus be of great use to both horse owners and veterinarians. The aim of this study was to provide information on equine patients referred to the CNC service of the University of Turin and to provide standard dietary protocols as used in our Veterinary Teaching Hospital for the most common nutrition-re...
Evaluation of an in vitro system to simulate equine foregut digestion and the influence of acidity on protein and fructan degradation in the horse’s stomach.
Journal of animal physiology and animal nutrition    June 20, 2017   Volume 101 Suppl 1 51-58 doi: 10.1111/jpn.12635
Strauch S, Wichert B, Greef JM, Hillegeist D, Zeyner A, Liesegang A.The aim of this study was to improve an in vitro system in order to gather optimized information on the digestion of different forages in the horse's upper gastrointestinal tract. Therefore, foregut digestion of several forages was simulated in vitro (Part 1). The effect of different pH values on in vitro fructan degradation of two selected grasses (Part 2) was tested subsequently. Part 1: We hypothesized that our system produces representative results simulating digestive processes in the upper alimentary tract, but neglects microbial fermentation. In vitro digestion of six forages (grass ...
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