Analyze Diet

Topic:Organic Matter

Organic matter in the context of equine health refers to decomposed plant and animal material found in soil, which contributes to nutrient availability and soil structure. It plays a role in maintaining pasture quality, which directly impacts the diet and overall health of horses. Organic matter influences soil water retention, aeration, and microbial activity, all of which are important for the growth of forage consumed by horses. This page compiles peer-reviewed research studies and scholarly articles that explore the composition, benefits, and management of organic matter in equine environments, as well as its implications for horse nutrition and health.
Feed intake, digestibility and passage kinetics in grazing horses.
Scientific reports    January 22, 2026   Volume 16, Issue 1 3052 doi: 10.1038/s41598-026-35647-7
Bachmann M, Bochnia M, Wensch-Dorendorf M, Glatter M, Schäfer S, Simroth K, Greef JM, Zeyner A.Feed intake and digestibility are important indicators for sufficient nutrient supply, but they can be assessed only to a limited extent in horses on pasture. In horses, feed intake is embedded in a complex behavioural pattern of searching, selecting, chewing and almost constant movement called foraging. The objectives of this study were to estimate organic matter intake (OMI) and organic matter digestibility (OMD) in six horses, 24 h/day on pasture, based on plant alkanes and synthetic n-hexatriacontane (C) excretion. A multi-compartmental model was fitted to the marker excretion and the C m...
The Effects of the Lactation Period, Mare Age, and Foaling on the Chemical and Physical Composition of Milk from Kazakh Mares Kept Under Natural Pasture Conditions.
Animals : an open access journal from MDPI    June 19, 2025   Volume 15, Issue 12 1817 doi: 10.3390/ani15121817
Toishimanov M, Zhanten O, Kanat R, Beishova I, Ulyanov V, Assanbayev T, Sharapatov T, Daurov D, Daurova A, Sapakhova Z, Nametov A, Shamekova M.This study aimed to evaluate the chemical composition of milk from Kazakh mares kept under free-grazing conditions on natural pasture without supplementary feeding. Fifty Kazakh mares were aged 3 to 13 years, kept in herds, and categorized by age, number of foalings, and lactation stage for statistical analysis. Milk samples were collected, chilled, and analyzed on the same day using a Milkoscan FT2. The composition of fat, protein, lactose, total solids, casein, and other components was examined. Statistical analyses, including ANOVA, PCA, CDA, and Pearson correlation analysis, were performed...
Targeted pretreatment and inoculation strategies for horse manure fermentation: Impact on metabolites and microbial community composition.
Journal of environmental management    May 21, 2025   Volume 387 125894 doi: 10.1016/j.jenvman.2025.125894
Pipereau K, Trably E, Santa-Catalina G, García-Bernet D, Carrere H.Horse manure is a lignocellulosic biomass found in significant quantities with a vast indigenous flora, not yet fully valorized apart from anaerobic digestion. Its use in the fermentation process can lead to the production of higher-value metabolites. This study investigates three inoculation strategies coupled with five pretreatment conditions for horse manure fermentation. Two microwave pretreatments (200W and 1000W) were compared with a conventional thermal pretreatment, a thermo-acid pretreatment, and an unpretreated condition. The sole horse manure indigenous microorganisms were used in f...
Potential microbial strains for composting cattle and horse manure.
Brazilian journal of biology = Revista brasleira de biologia    May 9, 2025   Volume 85 e292065 doi: 10.1590/1519-6984.292065
The potential of effective microorganisms in composting animal manure might be significant. This work aimed at isolating promising microbial strains for composting cattle and horse manure. A total of forty-five isolates have been isolated from soils of Northern Kazakhstan as well as cattle and horse manure. These microorganisms were extensively studied for their cellulose-degrading capacity, non-pathogenicity, protease, nitrogenase and catalase activities, as well as their growth stimulation, antagonistic ability, and growth rate. As a result, 21 potent strains were selected and genotyped for ...
Trophic rewilding by large herbivores reduces plant nitrogen and water limitation across seven sites irrespective of their edaphic conditions.
The Science of the total environment    March 20, 2025   Volume 973 179141 doi: 10.1016/j.scitotenv.2025.179141
Kaštovská E, Mastný J, Choma M, Čapek P, Jirků M, Bitomský M, Konvička M.Based on a growing understanding of the role of wild megafauna in the functioning of natural ecosystems, trophic rewilding by large herbivores is increasingly used as a nature-based solution to mitigate biodiversity loss and climate change in Europe and beyond. Despite the growing interest in implementing nature-based approaches to restore key non-productive ecosystem services, there is relatively little data available to assess the benefits and risks of rewilding projects. We therefore investigated the effects of year-round grazing by large ungulates on plant biomass characteristics and their...
Equine upper airway laser surgery generates plumes containing hazardous components.
American journal of veterinary research    February 6, 2025   1-6 doi: 10.2460/ajvr.24.11.0338
Sholty KA, Hecobian A, Sullivan AP, Collett JL, Koch DW, Hackett ES.To evaluate plume composition during simulated diode laser equine upper airway surgery. Unassigned: A 980-nm diode laser fiber was applied in continuous-wave mode in contact with laryngeal and pharyngeal tissues of 6 equine cadaver heads, and resultant plumes were collected for analysis. Measured parameters included the real-time concentration of particulate matter under the size of 2.5 μm (PM2.5), organic carbon (OC), elemental carbon (EC), and volatile organic compounds (VOCs). Unassigned: Visible smoke was encountered during surgical laser application and plume collection. Diode laser medi...
Estimating Total-Tract Digestibility of Nutrients and Their Contribution to Digestible Energy Supplies in Equine Diets.
Journal of equine veterinary science    January 23, 2025   105362 doi: 10.1016/j.jevs.2025.105362
Webster AP, Price T, Ingersoll T, Suagee-Bedore JK, White RR.Our objectives were to use a quantitative literature review to explore dietary and feed factors influencing apparent total-tract digestibility of dry matter (DMD), crude protein (CPD), neutral detergent fiber (NDFD), ether extract (EED), non-structural carbohydrates (NSCD), non-fiber carbohydrates (NFCD), and residual organic matter (rOMD) in equine diets, and to assess their contributions to digestible energy (DE) supplies. Data from 54 studies were modeled using linear mixed-effect regressions, with publication as a random effect to account for study variability. For each nutrient, five mode...
Biochar captures ammonium and nitrate in easily extractable and strongly retained form without stimulating greenhouse gas emissions during composting.
Journal of environmental quality    October 9, 2024   doi: 10.1002/jeq2.20634
Busch F, Leal ODA, Siebers N, Brüggemann N.During composting of organic waste, nitrogen is lost through gaseous forms and ion leaching. Biochar has been shown to capture mineral nitrogen (N: NH and NO ) from compost, which we hypothesize reduces NO formation. However, associating N captured by biochar with the dynamics of NO and other greenhouse gas (GHG) emissions during composting remains unstudied and was the aim of this work. We composted (outdoor for 148 days) together kitchen scraps (43.3% dw, where dw is dry weight), horse manure (40.9% dw), and wheat (Triticum aestivum L) straw (15.8% dw) without (Control) or with biochar (Bc,...
Extraction and Physicochemical Characterization of Hydroxyapatites From Horse Humerus Bones of Different Ages (1, 3, 6, and 8 Years old) Calcined at Low Temperature.
Journal of biomedical materials research. Part B, Applied biomaterials    September 19, 2024   Volume 112, Issue 10 e35484 doi: 10.1002/jbm.b.35484
Correa-Piña BA, Castillo-Paz AM, Davila U, Rodriguez-Garcia ME.The aim of this work is to investigate the changes in the physicochemical properties of hydroxyapatite (HAp) extracted from horse humerus bones of different ages (1, 3, 6, and 8 years) subjected to low temperature calcination (600°C). Thermal analysis revealed significant mass loss due to water, collagen, organic compounds, carbonates, and age-related magnesium out-diffusion. Higher fat content in older bones contributed to increased mass loss. Phosphorus content remained constant across age groups, while calcium and sodium showed age-related fluctuations. Magnesium levels decreased with ag...
Tropical grass digestibility assessed by the mobile bag and in vitro methods in horses.
Journal of equine veterinary science    June 4, 2024   105112 doi: 10.1016/j.jevs.2024.105112
Silva AH, Lucena JEC, Taran FMP, Cunha DS, Almeida JAT, Lima RS, Dias WS, Santiago JM.This study aimed to compare the digestibility of tropical grasses by horses by the in vivo method using mobile nylon bags with the in vitro digestibility method using horse feces as a source of inoculum. Five horses were used in a 2 × 5 factorial design with randomized blocks featuring two methods (in vivo and in vitro) and five grasses: Tifton 85 hay (Cynodon spp.), sixweeks threeawn grass (Aristida adsencionis, Linn), Alexandergrass (Brachiaria plantaginea (Link) Hitchc.), capim-de-raiz (Chloris orthonoton, Doell), and Sabi grass (Urochloa mosambicensis). No difference (P>0.05) was foun...
Comparison of forages’ digestion levels for different in vitro digestion techniques in horses
Veterinary medicine and science    February 19, 2024   Volume 10, Issue 2 e31373 doi: 10.1002/vms3.1373
Kara K, Altınsoy A.Forages are widely used in horse diets. Different in vitro techniques are being tried to determine the fermentation levels of forages in the horse digestive tract. Objective: This study aimed to evaluate the digestion levels of four dry forages commonly used in horse nutrition: alfalfa herbage, meadow hay, wheat straw, and Italian ryegrass. In vitro total digestion (TDT), in vitro Sunvold-large intestine digestion (SDT) and in vitro Menke-large intestine digestion (MDT) techniques were compared. Methods: The study determined in vitro true dry matter digestion (T-DMD), in vitro true organic mat...
Dietary Transitions Toward Sustainable Horse Feeding.
Journal of equine veterinary science    July 3, 2023   Volume 128 104880 doi: 10.1016/j.jevs.2023.104880
Karasu GK, Rogers CW, Gee EK.Sustainability is the balancing act of optimizing the use of current resources without compromising the current or future environment. Within the agriculture sector the primary focus of sustainability has been to reduce environmental pollution, specifically greenhouse gasses (GHG) emissions, nitrogen emissions, and leaching. For the equine industry the first step towards sustainability is the documentation and critique of current feeding and management practices to permit modifications to enable the industry meet social and legislative obligations. As a monogastric hindgut fermenter on a per k...
Bacteria and antimicrobial resistance profile during the composting process of wastes from animal production.
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]    February 9, 2023   Volume 54, Issue 2 1157-1167 doi: 10.1007/s42770-023-00912-8
Ferreira PFA, Xavier JF, Nunes JF, Fonseca IP, de Mattos de Oliveira Coelho S, Soares de Souza MM, da Silva Coelho I.Livestock waste is widely used in agriculture. Although they provide benefits to the soil, and consequently to plants, they have the potential to contaminate the environment, as they contain pathogenic microorganisms and determinants of antimicrobial resistance, if not properly managed. Therefore, this study aims to evaluate the effect of composting horse bedding and poultry litter in organic and conventional production systems on the occurrence of bacteria in the Enterobacteriales order and to identify their antimicrobial resistance profiles. Bacterial strains were isolated from Salmonella-Sh...
Horse manure as resource for biogas and nanolignocellulosic fibres.
Bioresource technology    January 27, 2023   Volume 372 128688 doi: 10.1016/j.biortech.2023.128688
Weiland K, Alge K, Mautner A, Bauer A, Bismarck A.Nanofibrillated cellulose (NFC) has key applications in composites, water filters and as emulsifiers. The affinity of NFC to water is a challenge, as it negatively influences its integrity. Lignin, a major component of plant biomass, is a natural hydrophobiser. Anaerobic digestion (AD) of biomass to produce biomethane allows to up-concentrate lignin in the fermentation residue containing lignocellulosic fibres. Horse manure was used as substrate for biogas production from which nanolignocellulose fibres (LCNF) were extracted. A biogas yield of 207 L kg with a methane concentration of 65 % was...
No size-dependent net particle retention in the hindgut of horses.
Journal of animal physiology and animal nutrition    July 27, 2022   Volume 106, Issue 6 1356-1363 doi: 10.1111/jpn.13757
Schwarm A, Clauss M, Ortmann S, Jensen RB.Sieve analyses of hindgut contents of horses as well as observations in horses where plastic markers had been applied to a caecal cannula suggested that there may be a discrimination by particle size in the passage or retention of digesta. Here, we performed a similar experiment with five caecum-cannulated horses (562 ± 31 kg) fed a constant amount (6.81 kg dry matter/day) of grass hay. Passage markers representing the liquid (Co-EDTA) as well as the particulate digesta phase (Yb-undefined; Cr mordanted fibre 1-2 mm; Ce-mordanted fibre 8 mm) were given as a pulse-dose into the can...
Effect of Mid-Term Dietary Administration of the Caesalpinia coriaria Extract on the Sustainable Mitigation of Equine Fecal Methane, Carbon Monoxide and Hydrogen Sulfide Production.
Journal of equine veterinary science    May 21, 2022   Volume 115 104021 doi: 10.1016/j.jevs.2022.104021
Acosta JAD, Elghandour MMMY, Mariezcurrena-Berasain MD, Adegbeye MJ, Fajemisin AN, Pliego AB, Salem AZM.This study aimed to evaluate the dietary administration of the Caesalpinia coriaria (CC) extract for 30 days on in vitro fecal greenhouse gases production. Fecal samples, as inoculums, were collected from horses given daily 0- (Fecal 0), 60- (Fecal 60) and 120- (Fecal 60) mL CC aqueous extract per animal. The extract dose was mixed with the morning feeding diet at 6:00 h for each horse. During incubation, 0-, 0.6-, 1.2- and 1.8-mL CC extracts were added to the basal diet which was fed to horses (as subtract) and evaluated with each fecal type. Feces from the horses given no CC extract produc...
A high-starch vs. high-fibre diet: effects on the gut environment of the different intestinal compartments of the horse digestive tract.
BMC veterinary research    May 19, 2022   Volume 18, Issue 1 187 doi: 10.1186/s12917-022-03289-2
Raspa F, Vervuert I, Capucchio MT, Colombino E, Bergero D, Forte C, Greppi M, Cavallarin L, Giribaldi M, Antoniazzi S, Cavallini D, Valvassori E....Horses are often fed high amounts of starch in their diets despite the well-established benefits of a fibre-based diet to promote gut health and animal welfare. The aim of the present study was to compare the effects of two different diets - one based on high amounts of starch (HS) vs. one base on high amounts of fibre (HF) - on specific parameters of the gut environment across different intestinal compartments of the horse digestive tract. To this end differences in the gastrointestinal environment between HS vs. HF fed horses were assessed in terms of dry matter, organic matter and ash conte...
Assessment of a full-scale solid-state anaerobic co-digestion: A multi-component substrate analysis by using ORWARE.
Waste management (New York, N.Y.)    May 10, 2022   Volume 146 36-43 doi: 10.1016/j.wasman.2022.04.042
Carlos-Pinedo S, Wang Z.Long-term sustainable biogas production requires different raw material alternatives, especially when reducing the most desirable organic substrate, food waste, which has been set as a goal in the 2030 Agenda. In Sweden, horse manure (HM) is generated in large quantities, and due to its physical and chemical characteristics, it has the potential to be used as a raw material to produce biogas through anaerobic digestion (AD). In order to investigate the challenges that HM digestion can impose in terms of methane yield and/or digestate quality, the modified ORganic WAste REsearch (ORWARE) AD mod...
Characteristics and Sodium Pentobarbital Concentrations of Equine Mortality Compost Piles in the Upper Midwest.
Journal of equine veterinary science    April 29, 2022   Volume 114 104000 doi: 10.1016/j.jevs.2022.104000
Lochner HL, Hutchinson ML, Wilson ML, Bianco AW, Johnston LJ, Prigge JL, Martinson KL.Recent changes in rendering availability for chemically-euthanized animals have created a need to explore composting as an equine mortality management method. The objectives of this study were to evaluate equine mortality composting in the Upper Midwest during fall and spring, document sodium pentobarbital concentrations throughout the process, and determine nutrient content of finished compost. During each season, four horses were euthanized by intravenous administration of sodium pentobarbital. Carcasses were positioned at the center of a woodchip and shavings base and covered with a 2:1 mix...
The In Vitro Digestion and Fermentation Characteristics of Feedstuffs Inoculated With Cecal or Colic Fluid of Dezhou Donkey.
Journal of equine veterinary science    January 10, 2022   Volume 110 103864 doi: 10.1016/j.jevs.2022.103864
Zhang Z, Wang Y, Zhu M, Wang C.The present study was conducted to evaluate the in vitro dry matter disappearance (IVDMD) and fermentation characteristics of 6 fibrous feedstuffs incubated with donkey cecal or colic microorganisms. The fibrous feeds were corn straw, wheat straw, peanut vine, peanut shell, wheat shell and wheat bran (WB), which are commonly applied in large-scale donkey farms in China. After 48 hours fermentation, the highest IVDMD occurred in WB, and the lowest occurred in peanut shell (P < .05) regardless what inoculum applied. The IVDMD was positively correlated with OM (R = 0.42, P < .01), CP (R ...
Mare’s Milk: Composition, Properties, and Application in Medicine.
Archives of Razi Institute    October 31, 2021   Volume 76, Issue 4 1125-1135 doi: 10.22092/ari.2021.355834.1725
Musaev A, Sadykova S, Anambayeva A, Saizhanova M, Balkanay G, Kolbaev M.Mare's milk is a highly valuable organic substance that has a great potential to replace cow's milk. Consumption of cow's milk causes digestive disorders in some individuals. Immunoglobulin E (IgE)-mediated cow's milk allergy (CMA) is one of the most common food allergies among infants. Therefore, finding a protein substitute with the same nutritional value is a priority. Mare's milk can be a good substitute for cow's milk, especially for those suffering from CMA. Prerequisites for this study were the recent interest in mare's milk, as an ancient relic of the Turkic peoples which contains lots...
The Effect of Three Levels of Concentrate and Grain Processing on Feeding Behavior, Nutrient Digestibility, Blood Metabolites and Fecal pH Of Turkmen Horses.
Journal of equine veterinary science    June 15, 2021   Volume 104 103690 doi: 10.1016/j.jevs.2021.103690
Kalantari RK, Rouzbehan Y, Fazaeli H, Direkvandi E, Salem AZM.The aim of this study was to investigate, the effect of different levels of concentrates and grain processing on feeding behavior, nutrient digestibility, fecal pH and blood metabolites in the horse. Sixteen 5 to 11 years old Turkmen horses with an initial body weight 433±50 kg were used in this experiment based on completely randomized design. Four treatments were studied, in three treatments were used 20, 25 and 30% of concentrate containing processed grains (A20, A25 and A30, respectively), and in one treatment was used 25% of concentrate containing whole grain (B25). The amount of feed in...
Effective energy exploitation from horse manure combustion.
Waste management (New York, N.Y.)    May 15, 2021   Volume 128 243-250 doi: 10.1016/j.wasman.2021.04.035
Da Lio L, Castello P, Gianfelice G, Cavalli R, Canu P.We investigated the exploitation of horse manure for energy recovery by combustion. First, the impact of the waste origin and management (storage conditions) was evaluated in terms of elemental analysis, moisture content, heating value, and ash melting temperature. Besides some carbon loss over the time, horse manure origin and management policy do not impact its profitable exploitation by combustion. More relevant, the ignition tests disproved the current industrial opinion that combustion of manure alone is difficult, without the addition of auxiliary fuel, like wood shavings. It has been de...
Effects of crude protein content on intake and digestion of coastal bermudagrass hay by horses.
Translational animal science    April 29, 2021   Volume 5, Issue 3 txab073 doi: 10.1093/tas/txab073
Spurgin CL, Coverdale JA, Leatherwood JL, Redmon LA, Bradbery AN, Wickersham TA.This study was conducted to determine the effects of forage crude protein () level on intake and digestion of Coastal bermudagrass hay by horses. Four cecally fistulated geldings were used in a 4 × 4 Latin square design with four treatments and four periods. Horses were fed one of four Coastal bermudagrass hays consisting of 7%, 10%, 13%, or 16% CP during each of the four 15-d periods. Intake and apparent digestibility were determined for each horse at the end of each period by total fecal collection. In addition, cecal fluid and blood samples were collected on the last day of each period for...
Exogenous enzyme amendment accelerates maturity and changes microflora succession in horse and wildlife animal manure co-composting.
Environmental science and pollution research international    January 7, 2021   Volume 28, Issue 17 21610-21620 doi: 10.1007/s11356-020-11568-4
Du G, Feng W, Cai H, Ma Z, Liu X, Yuan C, Shi J, Zhang B.Composting has been a rational method to dispose of horse or wildlife animal manures, especially in the developed cities with horse clubs and wildlife parks. However, few studies have focused on the mechanism and improvement methods for composting the horse or wildlife animal manures. In this study, we investigated the effect of exogenous compound enzymes on thermophilic composting, which could potentially support the management of horse and wildlife animal manures. With the presence of exogenous enzymes, the duration of high temperature (> 60 °C) was significantly prolonged (p <...
Methodical considerations when estimating nutrient digestibility in horses using the mobile bag technique.
Animal : an international journal of animal bioscience    December 10, 2020   Volume 15, Issue 1 100050 doi: 10.1016/j.animal.2020.100050
Thorringer NW, Jensen RB.Total collection of faeces is considered the golden standard for estimating apparent total tract digestibility (ATTD) in horses. However, the evaluation of individual feedstuffs is limited and determination of nutrient digestibility in different segments of the gastrointestinal tract (GIT) is excluded. The rationale for performing this study was that the mobile bag technique (MBT) can provide information on individual feedstuffs' degradation, and the use of fistulated animals does provide additionally information regarding degradation in individual segments of the GIT. Recommendations for usin...
The effect of supplementing pony diets with yeast on 1. In vivo and in vitro digestibility, faecal pH and particle size.
Animal : an international journal of animal bioscience    June 25, 2020   Volume 14, Issue 12 2481-2492 doi: 10.1017/S1751731120001524
Garber A, Hastie PM, Farci V, Bulmer L, Alzahal O, Murray JMD.Fibre is essential to maintain healthy gut; however, energy demands of performance horses can be too high to be met by forages alone. Yeast may support the function of cellulolytic bacteria to digest fibre. The aim of this work was to determine the effect of an oral supplement (VistaEQ) containing 4% live yeast on the in vitro and in vivo digestibility of high-starch (HS) and high-fibre diets (HF). Eight ponies were used in a 4 × 4 Latin square design consisting of 4- × 19-day periods and four diets: HF, HF + yeast (HFY), HS and HS + yeast (HSY). In vivo apparent digestibility (AD) was estim...
Evaluation of equine rectal inoculum as representative of the microbial activities within the horse hindgut using a fully automated in vitro gas production technique system.
Journal of animal science    February 23, 2020   Volume 98, Issue 3 skaa050 doi: 10.1093/jas/skaa050
Kujawa TJ, van Doorn DA, Wambacq WA, Hesta M, Pellikaan WF.The in vitro gas production technique (IVGPT) has been a valuable tool in ruminant nutrition research for decades and has more recently been used in horse nutrition studies to investigate fermentation activities of the equine hindgut though primarily using feces as inoculum. This study was conducted to evaluate the use of equine rectal content in the IVGPT system as a viable inoculum that can be considered representative of the activities throughout the equine hindgut. Additionally, the study was conducted to measure the effects on fermentation kinetics and end-product production using inoculu...
Owner Compliance to an Environmental Management Protocol for Severe Equine Asthma Syndrome.
Journal of equine veterinary science    January 23, 2020   Volume 87 102937 doi: 10.1016/j.jevs.2020.102937
Simões J, Sales Luís JP, Tilley P.Severe equine asthma (SEA) syndrome is a chronic recurrent respiratory disease, common among adult horses. The disease occurs in genetically susceptible individuals after their exposure to organic dust. Thus, environmental management has proved essential in controlling airway challenge and disease exacerbation. This is a demanding process that can only be achieved through the horse owners' cooperation. One year after initial diagnosis of SEA in a group of 39 horses, owner compliance to an environmental management protocol was evaluated. The overall compliance to the protocol was poor and the h...
Comparison of three systems for predicting the digestible energy value of natural grassland and lucerne hays for horses.
Animal : an international journal of animal bioscience    December 26, 2019   Volume 14, Issue 7 1413-1421 doi: 10.1017/S1751731119003069
Martin-Rosset W, Andueza D, Vermorel M.The accuracy and precision of the National Research Council (NRC), Gesellschaft für Ernährungsphysiologie (GfE) and Institut National de la Recherche Agronomique (INRA) systems for predicting the digestible energy (DE) value of hays were determined from the results of 15 digestibility trials with natural grassland hays and 9 digestibility trials with lucerne hays that all met strict experimental and a tight corpus of methods. The hays were harvested in the temperate zone. They covered broad ranges of chemical composition and DE value. The INRA system was more accurate than the other two syst...
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