Analyze Diet

Topic:Veterinary Science

Veterinary science and horses encompass the study and application of medical, surgical, and therapeutic practices to maintain and improve the health and welfare of equines. This field addresses a wide range of topics, including disease prevention, diagnosis, and treatment, as well as nutrition, reproduction, and behavior. Research in veterinary science for horses often involves understanding the pathophysiology of equine-specific diseases, developing advanced diagnostic techniques, and improving treatment protocols. This page gathers peer-reviewed research studies and scholarly articles that explore various aspects of veterinary science related to horses, providing insights into the latest advancements and methodologies in equine healthcare.
Detection of ragwort alkaloids in toxic hay by liquid chromatography/time-of-flight mass spectrometry.
The Veterinary record    November 10, 2009   Volume 165, Issue 19 568-569 doi: 10.1136/vr.165.19.568
Crews C, Anderson WA.No abstract available
[Health effects of occupational endotoxin exposure: a review and relevance to veterinary practice].
Tijdschrift voor diergeneeskunde    November 7, 2009   Volume 134, Issue 20 840-846 
Smit LA, Wouters IM, Heederik D, Douwes J.Endotoxins are cell-wall components of Gram-negative bacteria that are commonly present in plants and plant products and in faecal matter. This review presents an overview of endotoxin exposure levels, associated health effects, and relevance regarding veterinary practice. Exposure to airborne endotoxin is especially high in the agricultural sector and among veterinarians, and in particular among those working with horses or farm animals. Inhalation of endotoxins may cause acute airway inflammation and respiratory symptoms that can lead to (non-allergic) asthma and chronic obstructive pulmonar...
Probing the calcium and sodium local environment in bones and teeth using multinuclear solid state NMR and X-ray absorption spectroscopy.
Physical chemistry chemical physics : PCCP    November 7, 2009   Volume 12, Issue 5 1081-1091 doi: 10.1039/b915708e
Laurencin D, Wong A, Chrzanowski W, Knowles JC, Qiu D, Pickup DM, Newport RJ, Gan Z, Duer MJ, Smith ME.Despite the numerous studies of bone mineral, there are still many questions regarding the exact structure and composition of the mineral phase, and how the mineral crystals become organised with respect to each other and the collagen matrix. Bone mineral is commonly formulated as hydroxyapatite, albeit with numerous substitutions, and has previously been studied by (31)P and (1)H NMR, which has given considerable insight into the complexity of the mineral structure. However, to date, there has been no report of an NMR investigation of the other major component of bone mineral, calcium, nor of...
A comparative study of serological tests and PCR for the diagnosis of equine piroplasmosis.
Parasitology research    November 6, 2009   Volume 106, Issue 3 709-713 doi: 10.1007/s00436-009-1669-5
Jaffer O, Abdishakur F, Hakimuddin F, Riya A, Wernery U, Schuster RK.A total of 105 serum samples from endurance horses from different stables in Dubai were examined for the presence of antibodies against Theileria equi and Babesia caballi using immunofluorescence antibody test (IFAT) and competitive enzyme-linked immunosorbent assay (cELISA). A TaqMan real-time polymerase chain reaction (PCR) was used to detect DNA of piroplasms in specimens of clotted blood or EDTA blood samples of the same animals. Out of the 105 serum samples, the IFAT detected antibodies against T. equi in 35 (33.3%) cases while the cELISA gave 34 (32.4%) positive results. Eleven (10.5%) o...
Metabolic and hematological profiles in mature horses supplemented with different selenium sources and doses.
Journal of animal science    November 6, 2009   Volume 88, Issue 2 650-659 doi: 10.2527/jas.2009-1855
Calamari L, Abeni F, Bertin G.This study was conducted to determine the effects of dietary Se source and dose on metabolic and hematological profiles, and their relationships with oxidative status in horses. Twenty-five mature horses were blocked by BW and randomly allocated to 1 of 5 dietary treatments: negative control (CTRL: 0.085 mg of Se/kg of DM), 3 different dietary concentrations of organic Se provided by Se yeast (SY02, SY03, and SY04 containing 0.2, 0.3, and 0.4 mg of total Se/kg of DM, respectively), and 1 positive control provided by sodium selenite (SS03 containing 0.3 mg of total Se/kg of DM). Horses were fed...
A first report on the microbial colonisation of the equine oesophagus.
Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft    November 6, 2009   Volume 192, Issue 1 42-51 doi: 10.1016/j.aanat.2009.10.004
Meyer W, Kacza J, Schnapper A, Verspohl J, Hornickel I, Seeger J.Based on cryo-SEM, standard and high resolution TEM, glycoconjugate histochemistry, and microbiological differentiation, the present study demonstrates the colonisation of the epithelium of the equine oesophagus with microorganisms. As particularly apparent using cryo-SEM to illustrate natural conditions, the present microbiota were clearly dominated by bacteria, forming a one-layer system, as attached to and embedded in concentrated mannose/mannan substances covering the outer stratum corneal cells. Bacterial numbers ranged from 5600 to 7200 per mm(2) in the central part of the oesophagus, th...
Aspergillus fumigatus toxicity and gliotoxin levels in feedstuff for domestic animals and pets in Argentina.
Letters in applied microbiology    November 6, 2009   Volume 50, Issue 1 77-81 doi: 10.1111/j.1472-765X.2009.02756.x
Pena GA, Pereyra CM, Armando MR, Chiacchiera SM, Magnoli CE, Orlando JL, Dalcero AM, Rosa CA, Cavaglieri LR.To evaluate gliotoxin production by Aspergillus fumigatus strains isolated from feedstuff intended for domestic animals and pets, and to determine the amount of gliotoxin in these substrates. Results: A total of 150 feedstuff samples were collected. They were composed of 30 samples each of five different feed types (pigs, poultry, cattle, horse and pets). Aspergillus fumigatus gliotoxin production ability and gliotoxin presence in feedstuff was determined by HPLC. Aspergillus fumigatus strains were isolated from all of the tested samples. Strains from cattle, horses and pet food were able to p...
Heavy metal concentration in coat hair and hoof horn in stabled and reserve Polish Konik horses.
Polish journal of veterinary sciences    November 5, 2009   Volume 12, Issue 3 369-377 
Stachurska A, Wałkuska G, Chałabis-Mazurek A, Jaworski Z, Cebera M.The objective of the study was to determine if there were differences in Cr, Mn, Cu, Cd, Pb and Ni levels in coat hair and hoof horn between Polish Konik horses maintained in stabled and reserve systems in Roztocze National Park in Poland. Summer and winter feeding seasons (seasons of the year in case of hooves) and age of horses were taken into account. 107 samples of hair and hooves, as well as all kinds of feed and sources of water available for the horses were examined. The results of the study indicate that the concentration of the elements in the coat hair, hoof horn, feeds and water is ...
Evolutionary patterns of eastern equine encephalitis virus in North versus South America suggest ecological differences and taxonomic revision.
Journal of virology    November 4, 2009   Volume 84, Issue 2 1014-1025 doi: 10.1128/JVI.01586-09
Arrigo NC, Adams AP, Weaver SC.The eastern equine encephalitis (EEE) complex consists of four distinct genetic lineages: one that circulates in North America (NA EEEV) and the Caribbean and three that circulate in Central and South America (SA EEEV). Differences in their geographic, pathogenic, and epidemiologic profiles prompted evaluation of their genetic diversity and evolutionary histories. The structural polyprotein open reading frames of all available SA EEEV and recent NA EEEV isolates were sequenced and used in evolutionary and phylogenetic analyses. The nucleotide substitution rate per year for SA EEEV (1.2 x 10(-4...
Abdominal wall reconstruction using biological tissue grafts.
AORN journal    October 29, 2009   Volume 90, Issue 4 513-524 doi: 10.1016/j.aorn.2009.05.024
Brown P.Synthetic mesh products have been used to repair abdominal wall defects (eg, hernias) for many years. Biological mesh products are now available as an option when synthetic mesh products are not appropriate. To correctly prepare biological tissue grafts for use in the OR, perioperative nurses must understand the types of grafts available. Biological tissue grafts may be harvested from human, porcine, bovine, or equine hosts and from skin, pericardium, or small intestine submucosa.
Could work be a source of behavioural disorders? A study in horses.
PloS one    October 28, 2009   Volume 4, Issue 10 e7625 doi: 10.1371/journal.pone.0007625
Hausberger M, Gautier E, Biquand V, Lunel C, Jégo P.Stress at work, as shown by a number of human studies, may lead to a variety of negative and durable effects, such as impaired psychological functioning (anxiety, depression...). Horses share with humans this characteristic of working on a daily basis and are submitted then to work stressors related to physical constraints and/or more "psychological" conflicts, such as potential controversial orders from the riders or the requirement to suppress emotions. On another hand, horses may perform abnormal repetitive behaviour ("stereotypies") in response to adverse life conditions. In the present st...
Zonal chondrocyte subpopulations reacquire zone-specific characteristics during in vitro redifferentiation.
The American journal of sports medicine    October 21, 2009   Volume 37 Suppl 1 97S-104S doi: 10.1177/0363546509350978
Schuurman W, Gawlitta D, Klein TJ, ten Hoope W, van Rijen MH, Dhert WJ, van Weeren PR, Malda J.If chondrocytes from the superficial, middle, and deep zones of articular cartilage could maintain or regain their characteristic properties during in vitro culture, it would be feasible to create constructs comprising these distinctive zones. Objective: Zone-specific characteristics of zonal cell populations will disappear during 2-dimensional expansion but will reappear after 3-dimensional redifferentiation, independent of the culture technique used (alginate beads versus pellet culture). Methods: Controlled laboratory study. Methods: Equine articular chondrocytes from the 3 zones were expan...
Clinical and biochemical abnormalities in endurance horses eliminated from competition for medical complications and requiring emergency medical treatment: 30 cases (2005-2006).
Journal of veterinary emergency and critical care (San Antonio, Tex. : 2001)    October 14, 2009   Volume 19, Issue 5 473-478 doi: 10.1111/j.1476-4431.2009.00441.x
Fielding CL, Magdesian KG, Rhodes DM, Meier CA, Higgins JC.To describe the clinical and clinicopathologic abnormalities in endurance horses eliminated from competition and requiring emergency medical treatment. Methods: Retrospective study over a 2-year period (2005-2006). Ten horses that successfully completed the ride in 2006 were included for comparison. Methods: Temporary equine emergency field hospital. Methods: All horses (n=30) that were removed from endurance competition and treated for a metabolic abnormality were studied. Methods: Horses were treated with IV fluids and analgesics. Monitoring included lab work (PCV, total protein, and electro...
The molecular phylogeny of uterine serpins and its relationship to evolution of placentation.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology    October 13, 2009   Volume 24, Issue 2 526-537 doi: 10.1096/fj.09-138453
Padua MB, Kowalski AA, Cañas MY, Hansen PJ.Uterine serpins (USs), designated as SERPINA14, are expressed in the endometrium in response to progesterone. All species identified as having USs exhibit epitheliochorial placentation and are in the Ruminantia and Suidae orders of the Laurasiatheria superorder. The objective was to identify US genes in species within and outside Laurasiatheria and evaluate whether evolution of the US gene was associated with development of the epitheliochorial placenta. Through queries of nucleotide and genomic databases, known US genes were identified (caprine, bovine, porcine, water buffalo), and new US cod...
International Equine Ophthalmology Consortium (IEOC) Symposium.
Equine veterinary journal    October 7, 2009   Volume 41, Issue 6 606-607 doi: 10.2746/042516409x447789
Gilger BC, Brooks DE.This first IEOC symposium met its goals of gathering a group of leading equine ophthalmology clinicians and researchers to identify the challenges of the field. To facilitate collaboration, notes from round-table discussions, including the ideas and plans that were discussed are being complied and will be distributed to the attendees. Development of an IEOC membership organisation and website was discussed and supported by the group in an effort further to advance the science of equine ophthalmology. To present results from the collaborations made at this first IEOC meeting, an IEOC mini-sympo...
Influence of exercise and joint topography on depth-related spatial distribution of proteoglycan and collagen content in immature equine articular cartilage.
Equine veterinary journal    October 7, 2009   Volume 41, Issue 6 557-563 doi: 10.2746/042516409x424162
Brama PA, Holopainen J, van Weeren PR, Firth EC, Helminen HJ, Hyttinen MM.There is ample evidence on topographical heterogeneity of the principal biochemical components of articular cartilage over the surface of the joint and the influence of loading thereon, but no information on depth-related zonal variation in horses. Objective: To study depth-related zonal variation in proteoglycan (PG) and collagen content in equine articular cartilage. Methods: Two techniques (safranin-O densitometry and Fourier transform infrared spectroscopy) were applied to sections of articular cartilage from the proximal phalangeal bone of the metacarpophalangeal joint of 18-month-old Tho...
A zoonotic genotype of Enterocytozoon bieneusi in horses.
The Journal of parasitology    October 6, 2009   Volume 96, Issue 1 157-161 doi: 10.1645/GE-2184.1
Santín M, Vecino JA, Fayer R.This is the first report of Enterocytozoon bieneusi in an equid species. Feces from 195 horses from 4 locations in Colombia were examined for E. bieneusi by polymerase chain reaction. Of these, 21 horses (10.8%) were found positive for E. bieneusi . The prevalence of E. bieneusi in horses 1 yr of age (2.5%). No significant differences in prevalence were observed between male (13.7%) and female horses (9%). Sequencing of the internal transcribed spacer region of the SSUrRNA locus identified 3 genotypes. Two genotypes appear to be unique to horses and were named Horse 1 and Horse 2. A third geno...
Effects of fecal collection and storage factors on strongylid egg counts in horses.
Veterinary parasitology    September 30, 2009   Volume 167, Issue 1 55-61 doi: 10.1016/j.vetpar.2009.09.043
Nielsen MK, Vidyashankar AN, Andersen UV, Delisi K, Pilegaard K, Kaplan RM.Fecal analyses are becoming increasingly important for equine establishments as a means of parasite surveillance and detection of anthelmintic resistance. Although several studies have evaluated various egg counting techniques, little is known about the quantitative effects of pre-analytic factors such as collection and storage of fecal samples. This study evaluated the effects of storage temperature, storage time and airtight versus open-air storage on fecal egg counts. The experimental protocols were replicated in two study locations: Copenhagen, Denmark and Athens, Georgia, USA. In both loc...
Identification of 21 781 equine microsatellites on the horse genome assembly 2.0.
Animal genetics    September 26, 2009   Volume 41, Issue 2 222 doi: 10.1111/j.1365-2052.2009.01970.x
Mittmann EH, Wrede J, Pook J, Distl O.No abstract available
Equine parasites: diagnosis and control – a current perspective.
Parasites & vectors    September 25, 2009   Volume 2 Suppl 2, Issue Suppl 2 I1 doi: 10.1186/1756-3305-2-S2-I1
Traversa D.No abstract available
Restrictions of anthelmintic usage: perspectives and potential consequences.
Parasites & vectors    September 25, 2009   Volume 2 Suppl 2, Issue Suppl 2 S7 doi: 10.1186/1756-3305-2-S2-S7
Nielsen MK.Given the increasing levels of anthelmintic resistance in equine parasites, parasitologists now recommend traditional treatment approaches to be abandoned and replaced by more sustainable strategies. It is of crucial importance to facilitate veterinary involvement to ensure that treatment decisions are based on parasitic knowledge. Despite recommendations given for the past two decades, strategies based on the selective therapy principle have not yet been implemented on a larger scale in equine establishments. In contrast, treatment regimens appear to be derived from recommendations originally...
Ticks on domestic animals in Pernambuco, Northeastern Brazil. Dantas-Torres F.The objective of this article was to discuss some aspects of ticks associated with domestic animals in the State of Pernambuco, northeastern Brazil, based on a literature review and present new data obtained from recent tick collections carried out in this northeastern Brazilian state. From August 2007 to June 2008, 1,405 ticks were collected and five species were identified: Amblyomma cajennense (Fabricius, 1787), Amblyomma ovale Koch, 1844, Dermacentor nitens Neumann, 1897, Rhipicephalus (Boophilus) microplus (Canestrini, 1887), and Rhipicephalus sanguineus (Latreille, 1806). Dogs from urban...
Insect bite hypersensitivity in the horse: comparison of IgE-binding proteins in salivary gland extracts from Simulium vittatum and Culicoides nubeculosus.
Veterinary immunology and immunopathology    September 24, 2009   Volume 132, Issue 1 62-67 doi: 10.1016/j.vetimm.2009.09.016
Hellberg W, Mellor PS, Torsteinsdóttir S, Marti E.Insect bite hypersensitivity (IBH) is an IgE-mediated allergic dermatitis of horses caused by bites of insects such as Culicoides or Simulium spp. The aim of the present study was to compare the IgE-binding pattern of sera of IBH-affected horses to Culicoides nubeculosus and Simulium vittatum salivary gland extracts (SGE). Individual IgE responses to proteins of S. vittatum and C. nubeculosus SGEs were evaluated in 15 IBH-affected and three healthy horses on immunoblots. Fourteen out of the 15 IBH-affected but none of the healthy horses showed individual IgE binding patterns to seven and six m...
Multi-trait animal model estimation of genetic parameters for linear type and gait traits in the Belgian warmblood horse.
Journal of animal breeding and genetics = Zeitschrift fur Tierzuchtung und Zuchtungsbiologie    September 22, 2009   Volume 126, Issue 5 378-386 doi: 10.1111/j.1439-0388.2008.00798.x
Rustin M, Janssens S, Buys N, Gengler N.Genetic parameters for the height at withers, 27 linear type and six linear gait traits were estimated for the Belgian warmblood horse. Observations on 987 mares, mostly 3 years old, were analysed using a multi-trait animal model. The statistical model included appraiser, age and location (date x place of appraisal) as fixed effects. Genetic parameters were estimated using a canonical transformation and an expectation-maximization restricted maximum likelihood algorithm with an additional deceleration step. Estimates of heritability for the 33 linear traits were between 0.15 and 0.55. Heritabi...
The equine influenza epidemic in Australia: spatial and temporal descriptive analyses of a large propagating epidemic.
Preventive veterinary medicine    September 12, 2009   Volume 92, Issue 1-2 60-70 doi: 10.1016/j.prevetmed.2009.08.006
Cowled B, Ward MP, Hamilton S, Garner G.Australia experienced a large outbreak of equine influenza in August 2007. Nearly 10000 premises were infected during the epidemic. We used spatial and temporal analytical techniques to describe the epidemic, to quantify important descriptors of the epidemic, and to generate hypotheses about how the epidemic progressed and which control tools assisted in eradication. Spatio-temporal epidemic curves revealed three phases in the epidemic: dispersal, local spread and disease fade out. Spatial dispersal of infection rapidly declined immediately after national movement restrictions were introduced....
Purification and partial characterization of proteinase inhibitors of equine seminal plasma.
Reproductive biology    September 8, 2009   Volume 9, Issue 2 151-160 doi: 10.1016/s1642-431x(12)60023-0
Vasconcelos AB, Santos AM, Oliveira JS, Lagares Mde A, Santoro MM.The aims of the study were: 1/ to isolate and identify equine seminal plasma proteinase inhibitors, 2/ to evaluate their inhibitory potential, and 3/ to test a correlation between protein concentration in seminal plasma supernatant (obtained after precipitation with 36% ammonium sulfate) and stallion sexual maturity. Seminal plasma proteins obtained from six stallions were chromatographed in a Superose 12 (FPLC system) column followed by C(18) HPLC reverse-phase. Inhibition of trypsin amidase activity was evaluated in the collected fractions. Active proteins with a molecular mass of 6.3-7.0 KD...
Concentrations of dust and endotoxin in equine stabling.
The Veterinary record    September 8, 2009   Volume 165, Issue 10 293-295 doi: 10.1136/vr.165.10.293
Whittaker AG, Hughes KJ, Parkin TD, Love S.No abstract available
Direct injection horse urine analysis for the quantification and identification of threshold substances for doping control. III. Determination of salicylic acid by liquid chromatography/quadrupole time-of-flight mass spectrometry.
Analytical and bioanalytical chemistry    September 8, 2009   Volume 395, Issue 5 1403-1410 doi: 10.1007/s00216-009-3047-7
Vonaparti A, Lyris E, Panderi I, Koupparis M, Georgakopoulos C.In equine sport, salicylic acid is prohibited with a threshold level of 750 microg mL(-1) in urine; hence, doping control laboratories have to establish quantitative and qualitative methods for its determination. A simple and rapid liquid chromatographic/mass spectrometric method was developed and validated for the quantification and identification of salicylic acid. Urine samples after 900-fold dilution and addition of the internal standard (4-methylsalicylic acid) were directly injected to the liquid chromatography/quadrupole time-of-flight mass spectrometry system. Electrospray ionization i...
Lactobacillus and Bifidobacterium diversity in horse feces, revealed by PCR-DGGE.
Current microbiology    September 5, 2009   Volume 59, Issue 6 651-655 doi: 10.1007/s00284-009-9498-4
Endo A, Futagawa-Endo Y, Dicks LM.Lactobacillus equi, Lactobacillus hayakitensis, Lactobacillus johnsonii, and Weissella confusa/cibaria were the dominant species in 12 South African horses. The Bifidobacterium-group was detected in the feces of only one of the 12 horses. Sequencing of the nested-PCR amplicon identified the Bifidobacterium-group as Parascardovia denticolens. Cell numbers of L. equi, L. hayakitensis, and W. confusa/cibaria were consistent in all samples. P. denticolens, Bifidodobacterium pseudolongum, and a phylogenetic relative of Alloscardovia omnicolens were rarely detected. L. equigenerosi, a dominant speci...
Karyotypic relationships in Asiatic asses (kulan and kiang) as defined using horse chromosome arm-specific and region-specific probes.
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology    September 5, 2009   Volume 17, Issue 6 783-790 doi: 10.1007/s10577-009-9069-3
Musilova P, Kubickova S, Horin P, Vodicka R, Rubes J.Cross-species chromosome painting has been applied to most of the species making up the numerically small family Equidae. However, comparative mapping data were still lacking in Asiatic asses kulan (Equus hemionus kulan) and kiang (E. kiang). The set of horse arm-specific probes generated by laser microdissection was hybridized onto kulan (E. hemionus kulan) and kiang (E. kiang) chromosomes in order to establish a genome-wide chromosomal correspondence between these Asiatic asses and the horse. Moreover, region-specific probes were generated to determine fusion configuration and orientation of...