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Bacillus subtilis - Feed Ingredient

Bacillus subtilis is a spore-forming, Gram-positive bacterium used as a direct-fed microbial (probiotic) in equine feed and supplements. It is valued for its ability to withstand extreme conditions, including high temperatures and low pH, making it a suitable probiotic for inclusion in pelleted or processed feeds. The spores of Bacillus subtilis can survive the upper gastrointestinal tract and germinate in the hindgut, where they may support microbial balance and digestive function.

As a probiotic, Bacillus subtilis may contribute to the modulation of the gut microbiome through competitive exclusion of pathogens, production of antimicrobial compounds, and secretion of enzymes that assist in nutrient breakdown. The bacterium is also known for its ability to produce extracellular enzymes such as proteases and amylases, which may contribute to feed digestibility.

The following equine feeds, supplements and products contain Bacillus subtilis as an ingredient.

Feed Name	Brand	ID	Availability	Format	Protein	Fat	ADF	NDF	NSC	DE	Lysine	Calcium	Phosphorus	Magnesium	Potassium	Sodium	Chloride	Iron	Zinc	Copper	Selenium	Biotin	Vitamin E
4-In-1 (Meal)	MVP	469585		Premix	--	--	--	0%	--	--	--	--	--	--	--	--	--	--	2425.09 ppm	--	--	352.74 ppm	--
4-In-1 H/A Joint Supplement	MVP	469582	--	Pelleted	--	--	--	0%	--	--	--	--	--	--	--	--	--	--	1616.72 ppm	--	--	235.2 ppm	--
5-In-1 H/A	MVP	469573		Pelleted	--	--	--	0%	--	--	--	3.21%	1.56%	3.12%	1.1%	--	--	--	2658.84 ppm	886.28 ppm	16.5 ppm	146.67 ppm	3545.12 KIU
Achiever Foal Non-GMO	Daily Dose Equine	499929		--	11.11%	12.2%	21.89%	25.22%	--	3.68 mcal/kg	--	3.33%	1.67%	0.56%	--	--	--	--	944.44 ppm	316.67 ppm	1.11 ppm	--	2388.34 KIU
Achiever Non-GMO	Daily Dose Equine	499926		Textured	11.6%	8.4%	6.19%	17.07%	35.8%	3.77 mcal/kg	0%	4.7%	2.5%	0.21%	--	--	--	--	466.3 ppm	39.4 ppm	1.1 ppm	--	1081.5 KIU
Achiever-Lite	Daily Dose Equine	499927		Textured	10.5%	10.5%	20.7%	27.8%	--	3.49 mcal/kg	0%	4.7%	2.5%	0.21%	--	--	--	--	466.3 ppm	157.9 ppm	1.1 ppm	--	1083.8 KIU
Air-Way EQ	MVP	469314		Pelleted	--	--	--	0%	--	0 mcal/kg	7.86%	--	--	--	--	--	--	--	--	--	--	--	--
Alfa Pro	Hi-Pro Feeds	1188620	--	--	15.56%	--	34.72%	44.72%	--	2.59 mcal/kg	4.29%	1.11%	0.44%	--	--	--	--	--	77.78 ppm	22.22 ppm	0.33 ppm	--	171.47 KIU
Alfa-Pro Elite	Hi-Pro Feeds	1188630	--	--	16.67%	--	34.72%	44.72%	--	2.61 mcal/kg	4.67%	1.11%	0.44%	--	--	--	--	--	77.78 ppm	22.22 ppm	0.33 ppm	--	171.47 KIU
All-In-One Supplement	Extreme Equine Supplements	1120811		Powdered	20.6%	8.3%	9.4%	30%	1.2%	3.81 mcal/kg	16.43%	0.51%	0.33%	0.2%	3.33%	0.28%	3.67 ppm	327.6 ppm	2976.2 ppm	992.1 ppm	19.84 ppm	724.2 ppm	9920.6 KIU
Amber Grains Classic 12-4 Fiberwise	Coker Feed Mill	1234004		Textured	13.3%	4.4%	24.4%	46.7%	18.1%	2.74 mcal/kg	5.8%	1.11%	0.56%	0.33%	1.11%	0.2%	0.3 ppm	--	144.4 ppm	55.6 ppm	0.56 ppm	1.7 ppm	122.5 KIU
Amber Grains Classic 12-6 Textured	Coker Feed Mill	1233357	--	Textured	13.3%	6.7%	18.9%	36.7%	37.2%	3.16 mcal/kg	5%	1.11%	0.56%	0.33%	1.11%	0.2%	0.3 ppm	250 ppm	144.4 ppm	27.8 ppm	0.5 ppm	1.7 ppm	122.5 KIU
Amber Grains Classic Senior Textured	Coker Feed Mill	1233690		Textured	15.6%	7.8%	20%	37.8%	27.2%	3.14 mcal/kg	6.4%	1.11%	0.56%	0.33%	1.11%	0.2%	0.3 ppm	333.3 ppm	277.8 ppm	55.6 ppm	0.56 ppm	1.7 ppm	367.4 KIU
Amber Grains Elite 10-10 Performance	Coker Feed Mill	1232906	--	Textured	11.1%	11.1%	13.3%	27.8%	43.6%	3.71 mcal/kg	7%	1.11%	0.56%	0.33%	0.83%	0.3%	0.5 ppm	155.6 ppm	277.8 ppm	66.7 ppm	0.67 ppm	1.7 ppm	612.4 KIU
Amber Grains Elite 12-12 Performance	Coker Feed Mill	1233215	--	Textured	13.3%	13.3%	20%	37.8%	25.4%	3.55 mcal/kg	7.5%	1.11%	0.67%	0.33%	1.11%	0.3%	0.5 ppm	305.6 ppm	277.8 ppm	66.7 ppm	0.67 ppm	1.7 ppm	612.4 KIU
Amber Grains Elite Front Runner	Coker Feed Mill	1219085	--	Textured	15.6%	13.3%	16.7%	33.3%	26.9%	3.65 mcal/kg	6.8%	1.11%	0.67%	0.44%	1.11%	0.3%	0.5 ppm	277.8 ppm	277.8 ppm	66.7 ppm	0.67 ppm	1.7 ppm	612.4 KIU
Amber Grains Elite Mare and Foal	Coker Feed Mill	1233337	--	Textured	17.8%	8.9%	17.8%	34.4%	26.8%	3.29 mcal/kg	6.9%	1.39%	0.78%	0.44%	1.11%	0.3%	0.5 ppm	277.8 ppm	277.8 ppm	66.7 ppm	0.67 ppm	1.7 ppm	612.4 KIU
Amber Grains Elite Serenity Ultra LS	Coker Feed Mill	1232980	--	Pelleted	15.6%	11.1%	22.2%	37.8%	10%	3.35 mcal/kg	6.4%	1.11%	0.56%	0.33%	0.83%	0.3%	0.5 ppm	333.3 ppm	266.7 ppm	66.7 ppm	0.67 ppm	1.7 ppm	661.4 KIU
Amber Grains Elite Super Senior	Coker Feed Mill	1232890	--	Textured	15.6%	11.1%	17.8%	37.8%	21.8%	3.41 mcal/kg	6.8%	1.11%	0.67%	0.33%	0.83%	0.3%	0.5 ppm	333.3 ppm	277.8 ppm	66.7 ppm	0.67 ppm	1.7 ppm	612.4 KIU
Carb-Buster Non-GMO	Daily Dose Equine	499928		Premix	14.4%	12.2%	37.5%	47.5%	7.7%	3.07 mcal/kg	--	2.33%	1.33%	0.22%	--	--	--	--	944.4 ppm	316.7 ppm	1.11 ppm	--	1102.3 KIU
D-Carb Balance (Pellets)	MVP	469550		Pelleted	--	--	--	--	--	0 mcal/kg	--	5.62%	2.76%	5.88%	--	0.49%	0.76 ppm	--	4960.4 ppm	1234.59 ppm	24.25 ppm	--	5511.56 KIU
E-Clipse'PM Pellets	MVP	469570		Pelleted	5%	--	--	0%	--	--	146.97%	--	--	7.35%	--	--	--	--	2204.62 ppm	916.67 ppm	12.22 ppm	--	48888.9 KIU
Exceed 6-way	MVP	430454		Pelleted	--	--	--	0%	--	0 mcal/kg	18.3%	--	0%	2.2%	--	--	--	--	1322.77 ppm	440.92 ppm	17.64 ppm	132.28 ppm	6613.87 KIU
Freestyle Performance	Daily Dose Equine	499924		Textured	12.4%	12.4%	19.3%	39.1%	--	3.24 mcal/kg	0%	2.2%	0.82%	--	--	--	--	--	211.3 ppm	72.2 ppm	0.31 ppm	--	313 KIU
Gastro-Plex Pellets	MVP	469566		Pelleted	--	--	--	0%	--	--	--	--	--	--	--	--	--	--	2612.89 ppm	914.51 ppm	--	--	1224.79 KIU
GI Intensive Care	Bluebonnet Feeds	1230275	--	Powdered	--	--	--	--	--	0 mcal/kg	--	--	--	9.18%	--	--	--	--	--	--	--	--	--
Gut Powder	Uckele	401551		Powdered	--	--	--	--	--	--	--	--	--	--	--	--	--	--	--	--	--	--	--
GutBiome	ProElite	992345	--	Pelleted	--	--	--	--	--	--	--	--	--	--	--	--	--	--	--	--	--	--	--
Hedgerow Pronto Paste	BioStar	647538		Paste	2.56%	3.84%	3.84%	7.6%	--	3.72 mcal/kg	--	--	0.42%	0.13%	0.46%	2.04%	--	196 ppm	6.8 ppm	2.6 ppm	--	--	--
Vitamin & Mineral Supplement	Horse Guard	401284		Pelleted	10%	--	--	--	--	0.5 mcal/kg	--	0.7%	0.63%	0.3%	--	--	--	890.76 ppm	3563.03 ppm	267.23 ppm	53.45 ppm	80.17 ppm	17815.1 KIU
In-SYNC 4	MVP	430455		Pelleted	50%	30%	--	0%	--	0 mcal/kg	17.55%	--	0%	8.82%	--	--	--	--	793.66 ppm	264.55 ppm	17.64 ppm	--	35273.99 KIU
Infinity	Daily Dose Equine	1208301	--	Crumble	13.33%	--	15.22%	33.78%	--	3.53 mcal/kg	8.33%	1.67%	0.78%	0.33%	--	--	--	--	555.56 ppm	185.56 ppm	0.89 ppm	--	923.49 KIU
Mass-No-Sass	Daily Dose Equine	499925		Textured	14.2%	15.8%	24.6%	35.1%	--	3.77 mcal/kg	0%	1.9%	0.42%	--	--	--	--	--	240 ppm	76.8 ppm	0.32 ppm	--	319.6 KIU
Medicine Bag Complete	Stance Equine	1003686	--	Powdered	--	--	--	--	--	--	--	13.1%	5.3%	6.6%	0.2%	--	--	--	3707.1 ppm	1378.8 ppm	14.1 ppm	--	--
Mega Dose	Horse Guard	991506	--	Pelleted	100%	--	--	--	--	1 mcal/kg	0.53%	0.8%	0.53%	0.11%	--	--	--	926.3 ppm	1852.6 ppm	138.9 ppm	27.8 ppm	297 ppm	13923.9 KIU
Microbiome Support	Dynavyte	1022929	--	Liquid	--	--	--	--	--	--	--	--	--	--	--	--	--	--	--	--	--	--	--
Natur-Oat	Excel Equine Feeds	979169		--	15.6%	6.7%	7.2%	16.1%	--	3.59 mcal/kg	6.4%	1.1%	0.7%	--	--	--	--	--	199.4 ppm	66.7 ppm	0.7 ppm	--	318.4 KIU
Natural Digestive Support	BioEquine Supplement	71876		--	3.6%	--	--	--	--	0.16 mcal/kg	1.2%	3.03%	3.53%	3.03%	3.03%	0.66%	3.26 ppm	15105 ppm	4040.4 ppm	505.05 ppm	20.2 ppm	73.33 ppm	8888.89 KIU
Performance Senior	Daily Dose Equine	499922		Textured	12.4%	15.5%	27.5%	37.5%	23.8%	3.05 mcal/kg	0%	2.1%	0.82%	--	--	--	--	--	211.3 ppm	72.2 ppm	0.33 ppm	--	313 KIU
Prebiotic + Probiotic	Equine Choice	71740		Granular	10%	--	--	--	--	3 mcal/kg	--	--	--	--	--	--	--	--	--	--	--	--	19621.2 KIU
Prebiotic and Probiotic Paste	Equine Choice	529995		--	10%	--	--	--	--	--	--	--	--	--	--	--	--	--	--	--	0 ppm	--	7000 KIU
Refit	4996	1223316	--	Pelleted	20%	3.9%	8.9%	21.1%	--	1.3 mcal/kg	11.1%	3.33%	2.22%	--	--	--	--	--	666.7 ppm	222.2 ppm	2.22 ppm	--	2449.6 KIU
Sand Free (Pellets)	MVP	469547		Pelleted	2.5%	0.63%	6.88%	18.97%	--	3.42 mcal/kg	--	--	--	--	--	--	--	--	--	--	--	--	--
Senior Active +	Triple Crown	678992		Pelleted	15.6%	13.3%	24.4%	37.8%	13.2%	3.38 mcal/kg	6.8%	1.1%	0.7%	0.6%	1.1%	0.56%	0.56 ppm	166.7 ppm	244.4 ppm	55.6 ppm	0.56 ppm	0.5 ppm	416.4 KIU
Senior Plus	Excel Equine Feeds	748187	--	Pelleted	14.4%	11.1%	23.3%	41.1%	--	3.29 mcal/kg	3.8%	1.3%	0.7%	--	--	--	--	--	166.7 ppm	55.6 ppm	0.7 ppm	--	367.4 KIU
Senior-Flex H/A	MVP	469578		Pelleted	--	--	--	0%	--	--	0%	--	--	--	--	--	--	--	--	--	--	--	39123.6 KIU
Senior-Flex	MVP	469579		Premix	--	--	--	0%	--	--	0%	--	--	--	--	--	--	--	--	--	--	--	44092.49 KIU
SmartPerform Ultra Pellets	SmartPak	597046	--	Pelleted	--	--	--	--	--	--	--	3.37%	--	2.02%	--	--	--	--	--	--	--	--	--
Sweet 'N Safe	Daily Dose Equine	499923		Textured	12.4%	9.3%	9.4%	30%	23%	3.59 mcal/kg	0%	1.6%	0.83%	--	--	--	--	--	270.1 ppm	92.8 ppm	0.33 ppm	--	313 KIU
Synergize	Tribute	1012459	--	Pelleted	13.3%	14.4%	14.4%	31.1%	16.7%	3.69 mcal/kg	7.5%	0.88%	0.7%	0.3%	1.1%	0.4%	0.7 ppm	138.9 ppm	244.4 ppm	88.9 ppm	0.7 ppm	4.9 ppm	612.4 KIU
Trifecta	Horse Guard	430551		--	--	--	--	--	--	1.5 mcal/kg	--	0.3%	0.3%	0.2%	--	--	--	489.9 ppm	1959.7 ppm	367.4 ppm	14.7 ppm	156.8 ppm	7348.7 KIU
Yucca Saponin	Uckele	776943	--	Powdered	--	--	--	--	--	0 mcal/kg	--	--	--	--	--	--	--	--	--	--	--	--	--