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Topic:Animal Species
The topic "Animal Species and Horses" explores the interactions and relationships between horses and various other animal species. This includes understanding the ecological roles horses play within their environments, as well as their interactions with domestic and wild animals. Research in this area may cover aspects such as competition for resources, symbiotic relationships, and behavioral influences between horses and other species. Studies may also investigate the impact of horses on biodiversity and ecosystem dynamics. This page compiles peer-reviewed research studies and scholarly articles that examine these interspecies relationships, focusing on the ecological, behavioral, and environmental implications of horses interacting with other animals.
Musculoskeletal Assessment of Forelimbs and Hindlimbs in Mounted Police Horses using Infrared Thermography. Infrared thermography (IRT) has become established as a complementary tool in equine veterinary medicine, allowing for the early detection of thermal changes associated with musculoskeletal inflammatory processes. Objective: This study aimed to apply infrared thermography to characterize the surface thermal profile of the forelimbs and hindlimbs of mounted police horses, considering clinical classification, anatomical region, body side, and repeated evaluations over time. Methods: The research was conducted at the Military Police Cavalry in Manaus (AM), Brazil, involving 20 adult horses submit...
Oocyte and Embryo Vitrification in Horses. Vitrification has emerged as a decisive technique in assisted reproductive technology, offering superior cryopreservation outcomes for both oocytes and embryos compared with traditional slow freezing. This chapter provides a comprehensive overview of the vitrification of equine oocytes and embryos, emphasizing the influence of technical factors, including cryoprotectant selection, exposure time, temperature control, and device volume, on vitrification outcomes. Biological factors such as the oocyte maturation stage, the presence of cumulus cells, and embryo size are also explored, highlighting...
Cryopreservation of Bovine, Equine, and Porcine Sperm. In livestock breeding, cryopreserved semen is routinely used for artificial insemination. Sperm cryopreservation enables long-term storage and transport of insemination doses, thereby securing genetic material for later use. Although specifics may vary, commonly used sperm cryopreservation protocols for domestic species feature similar processing steps as outlined in this chapter. We here describe common procedures for cryopreservation of bull, stallion and boar sperm. This includes preparation and use of the following diluents: TRIS egg yolk extenders (TEY) for bull sperm, ordinary and freezi...
Beyond Body Condition Score: A Proposed Interspecies Framework for Tissue-by-Tissue Nutritional Assessment in Veterinary Practice. In clinical veterinary medicine, the Body Condition Score (BCS) serves as a validated, semi-quantitative estimation of subcutaneous adipose reserves, yet it cannot by itself detect systemic nutrient deficiencies or excesses that do not necessarily imply energy storage. Human clinical dietetics addressed an analogous diagnostic gap through the Nutrition-Focused Physical Examination (NFPE), but veterinary medicine still lacks a unified, multi-species physical examination sequence for nutritional screening. This narrative review synthesizes the comparative and clinical nutrition literature into a...
Exhaled breath condensate in horses: a first microbiological insight. Exhaled breath condensate (EBC) is a non-invasive method for obtaining samples reflecting the airway lining fluid; however, its microbiological composition remains poorly characterized. Objective: To perform an exploratory microbiological comparison of EBC in clinically healthy horses maintained under different environmental conditions. Methods: An observational study included 30 mares equally distributed into two groups: horses housed in stalls (Racetrack group) and horses maintained under pasture conditions (Farm group). EBC samples were collected using a validated device developed by the re...
Evaluation of tilapia skin (Oreochromis niloticus) frozen, frog skin (Rana catesbeiana), and tilapia skin (Oreochromis niloticus) sterilized with gamma rays dressings, in equine skin wound healing. Skin wounds are common in horses due to species-specific behavioral responses. Equine skin morphology, vascularization, and inflammatory responses to wounds are comparable to those in humans, supporting the translational relevance of equine wound-healing studies. Investigating biomaterials such as tilapia and frog skin may improve wound management. This study demonstrated that sterilized tilapia and frog skin exhibit promising wound-healing properties. Unassigned: Macroscopic, microscopic, and thermographic aspects of second-intention wound healing were compared in six horses with surgically i...
Thermographic assessment of surface temperature distribution and agreement between a thermographic camera, infrared thermometer, and rectal temperature in exercising horses under tropical climate conditions. Infrared thermography has become an increasingly valuable non-invasive imaging technique for monitoring physiological status and detecting abnormalities in horses. However, its application under tropical environmental conditions remains insufficiently investigated, particularly regarding the influence of ambient conditions on measurement accuracy and agreement with conventional temperature assessment methods. This study aimed to evaluate the agreement among a thermographic camera (TC), infrared thermometer (IR), and rectal temperature (RT), characterize body surface temperature distribution be...
Transcriptome Sequencing and Differential Expression Analysis of Ovaries from Kazakh Mares During Seasonal Quiescence and Estrus Activation. Kazakh horses are typical seasonal breeders, and ovarian activity is markedly reduced during the non-breeding period. Hormonal stimulation can induce ovarian functional responses during seasonal anestrus; however, the early ovarian transcriptional changes associated with this response remain incompletely understood. In this study, ovarian transcriptome sequencing was performed in 12 Kazakh mares examined during the seasonal anestrous period. The mares were classified into a non-hormonally stimulated seasonal quiescent group (DB, = 6) and a hormone-induced ovarian activation group (DY, = 6) f...
Fatal equine gas gangrene associated with Clostridium septicum in the Brazilian Amazon: clinicopathological and molecular characterization of a 20-case series. Clostridial gas gangrene is an acute necrotizing infection of skeletal muscle characterized by rapid tissue destruction and severe systemic toxemia in horses; however, systematically documented case series from tropical settings remain scarce. Here, we describe a clinicopathological and molecularly confirmed series of 20 fatal equine gas gangrene cases diagnosed in the eastern Brazilian Amazon (Pará State) between 2018 and 2026. Horses presented with abrupt onset of severe lameness, rapidly progressive swelling of a limb or muscle group, and palpable subcutaneous crepitus, frequently followin...
The Polish Konik Horse: A Multidisciplinary Review of Its Origin, Genetics, Ecology, Health, Behaviour and Reproductive Biology. The Polish Konik horse (PKH) is one of Europe's best-known native conservation breeds. Traditionally associated with the extinct Eurasian tarpan and conservation grazing, the breed has recently become the subject of multidisciplinary research encompassing genetics, ecology, health, behaviour and reproduction. This narrative review summarises current knowledge on the biological characteristics and contemporary scientific significance of the PKH. Literature published between 2005 and 2026 was identified through searches of PubMed, Scopus, Web of Science and Google Scholar and narratively synthes...
Exercise-Induced Acid-Base, Electrolyte, and Metabolic Responses in Colombian Creole Horses Across Different Gait Modalities. Exercise physiology in Colombian Creole Horses remains poorly characterized despite the breed's extensive use in athletic activities. The objective of this study was to evaluate exercise-induced changes in blood biochemical and gas parameters and to determine whether these responses differed according to sex, geographic origin, or gait modality. An analytical observational study with repeated measures was conducted in 76 athletic CCHs. Blood samples were obtained from the jugular vein at rest and immediately after exercise to measure acid-base indicators (pH, carbon dioxide pressure, bicarbona...
Limited Antibodies to Henipaviruses Detected Among Nigerian Horses Using Pseudotyped Virus Neutralization Tests. The Hendra virus (HeV; ) and Nipah virus (NiV; ), members of the genus , are high-consequence pathogens that have caused multiple zoonotic outbreaks with high mortality. Frequently, pigs or horses have acted as bridging hosts between bats, the reservoir hosts, and people. We used pseudotyped viruses with the fusion (F) and glycoprotein (G) envelope proteins of the NiV and Ghana virus (GhV; ) in neutralization tests (PVNTs) to screen serum samples from 138 apparently healthy horses in northwest Nigeria. No antibodies to GhV were detected in the 138 samples tested. Two serum samples were positiv...
Ortholog-specific Spatial Expression of Vanins in the Respiratory Tract of Horses. Equine asthma (EA) is the most prevalent chronic respiratory disease in horses, but molecules thought to be relevant in its pathogenesis are not yet fully understood. Recently, increased Vanin-1 (VNN1) levels were found in bronchoalveolar lavage fluid from horses with severe EA. Human VNN1 is known as a pantetheinase, which is associated with respiratory diseases such as asthma, but the equine vanins (eVNNs) are uncharacterized. We identified the domain architecture of eVNN in silico and analyzed its tissue expression in healthy horses using RT-PCR (=5) and in situ hybridization (=3). Recombin...
Phylogenetic and phylogeographic analysis of equine influenza in Colombia. In 2022, Colombia experienced an outbreak of equine influenza (EI) that primarily affected competition horses. Despite the epidemiological relevance of equine influenza virus (EIV) in the country, no previous phylogenetic or phylogeographic analyses based on complete genomes have been reported. Objective: To perform phylogenetic and phylogeographic analyses of complete EIV genomes obtained from horses with clinical signs in two Colombian regions between 2020 and 2023. Methods: Molecular epidemiological study based on whole-genome sequencing and evolutionary analyses. Methods: Nasopharyngeal sa...
Selective oncolytic activity of newcastle disease virus in equine sarcoid primary cells: a preclinical investigation. Equine sarcoids are the most common skin tumors in horses and are characterized by local invasiveness, high recurrence rates, and inconsistent responses to current therapies. Oncolytic virotherapy represents a promising alternative approach that exploits defects in antiviral signaling pathways in tumor cells. Newcastle disease virus (NDV), an avian paramyxovirus that is nonpathogenic in mammals, has demonstrated tumor-selective activity in several species; however, its effects on equine sarcoids have not been previously investigated. In this study, primary cell cultures derived from equine sar...
Development and Evaluation of an Indirect ELISA for Strangles Based on an Untagged EQ8-SclF Fusion Protein. Strangles, caused by subsp. (), is a highly contagious respiratory disease that causes substantial economic losses to the horse industry worldwide. Serological diagnosis currently relies mainly on the imported IDvet dual-antigen sandwich ELISA kit, which shows good diagnostic performance but is expensive and lacks a domestic alternative in China, thereby limiting its routine use in primary veterinary settings. The aim of this study was to develop a domestic indirect ELISA based on novel antigen targets and an untagged antigen purification strategy. Using comparative bioinformatics analyses, ...
Comparative genomics of Standardbred horses reveals candidate regions under selection for harness racing performance. Harness racing performance in horses is a complex polygenic trait influenced by genetic background, breeding history, training, and environment. Understanding genomic patterns shaped by long-term artificial selection provides insights into performance-related traits and breed-specific genomic variation. Objective: This study aimed to characterize genomic differentiation, population structure, and candidate genomic regions potentially influenced by historical selection in Standardbred horses using comparative whole-genome sequencing involving diverse horse breeds. Methods: Whole-genome sequenci...
Effect of sample storage on pH-value in equine faeces. Measurements of pH-value in equine faeces are often performed in studies on nutrition and health in horses, but a standard protocol for it is lacking despite the knowledge that different sample handling may affect the pH-value. Sample handling prior to pH-measurement is often determined by practical settings and has been reported to vary in published studies within the area. The purpose of the current study was therefore to investigate the influence of different combinations of storage time and temperature on the pH-value in equine faecal samples in comparison to immediate pH-measurement at de...
Comparative Analysis of Volatile Components Changes in Horse Fat During Dry Fractionation by E-Nose, HS/GC-IMS and SPME/GC-MS. Horse fat is a valuable lipid source for traditional meat products, but its flavor quality is remains unclear during processing conditions. To investigate the effect of dry fractionation on the volatile profile of refined horse fat, samples obtained at different temperature were analyzed using an E-nose, HS/GC-IMS, and SPME/GC-MS. The E-nose results showed that the volatile profiles of the samples differed markedly, with the solid fractions obtained at 25 and 15 °C (S25 and S15) exhibiting stronger sensor responses and clustering together, whereas the liquid fraction at 5 °C (L5) showed the ...
Ethical monitoring of ovum pick-up procedures in domestic horses: A standardised self-assessment checklist for responsible veterinary practice. Assisted reproductive technologies (ARTs) have the potential to accelerate genetic improvement in selective equine breeding programmes while addressing specific reproductive challenges. One recent assisted reproductive technique in domestic horse breeding is Ovum Pick-Up combined with Intracytoplasmic Sperm Injection (OPU-ICSI). Despite their advantages, the implementation of these new procedures, like more established breeding methods, raises ethical concerns relating to aspects like animal welfare, staff safety, professional and veterinary practice integrity, which must be monitored and asse...
Equine Blood and Salivary Cortisol Levels While Exercising on a Water Treadmill with an Artificial River System. A water treadmill (WT) is used in equine rehabilitation and training. This study aimed to investigate whether varying water depths and using an active artificial river (AR) system during water treadmill exercise would affect physiological stress responses in leisure horses. Thirteen leisure horses participated in five 20 min WT sessions, including those with a treadmill with dry belt, fetlock-depth WT (with/without AR), and carpal-depth WT (with/without AR) at a walking speed of 1.25 m/s. Blood and salivary cortisol levels were measured before, immediately after, and 30 min after exercise. Blo...
Allele frequencies of 7 inherited disorders in performance and random cohorts of American Quarter Horses (2020-2024). To estimate allele frequencies for genetic diseases in the American Quarter Horse (QH), including hyperkalemic periodic paralysis (HYPP), type 1 polysaccharide storage myopathy (PSSM1), malignant hyperthermia (MH), myosin-heavy chain myopathy (MYHM), glycogen branching enzyme deficiency (GBED), hereditary equine regional dermal asthenia (HERDA), and equine juvenile spinocerebellar ataxia (EJSCA), and to compare these frequencies over time among elite performance subgroups and a random cohort of registered horses. Unassigned: In this prospective genetic survey, DNA was extracted from 300 elite ...
Relationship Between Feed Olfactory Exploration and Consumption in Horses. The aim of the study was to evaluate the relationship between horses' behaviour during olfactory exploration and following consumption of the same food containing herbs, either previously exposed to the herb odour or not. Twenty horses' responses to 14 herbs were compared in a smelling test, when the horses sniffed a herb, and after 20 min a consumption test, when they consumed oats mixed with the same herb. Before the tests, the horses were exposed to the scent of seven herbs, whereas the remaining seven herbs were non-pre-exposed. The results show that horses consumed oats with herbal additi...
Comparative analysis of the karyotypes of Equus caballus and Equus asinus in the context of contemporary genomic data. Karyotypes of species within the genus Equus display considerable variation in chromosome number and are characterised by numerous structural rearrangements that have played a pivotal role in their evolutionary divergence and speciation. Equus caballus (horse), 2n = 64 and Equus asinus (donkey), 2n = 62, constitute particularly interesting models for comparative studies due to the diversified morphology of their chromosomes, including acrocentric and submetacentric pairs, documented Robertsonian translocations, and centric fusions. In recent decades, widely applied molecular cytogenetic techni...
Adaptive variation in butyrylcholinesterase-influenced lipid metabolism during horse domestication. Lipid metabolism influences horse athletic performance, yet its genetic basis remains unclear. Here, we investigate the genetic basis of lipid metabolic traits by quantifying key metabolic parameters and sequencing the genomes of 298 horses. We identify a missense mutation (ECA19:9,198,989, G>C) in the BCHE gene that is strongly associated with lipid metabolism, especially butyrylcholinesterase (BChE) activity. The "C" allele is associated with increased BChE activity and reduced intracellular triglyceride levels, hepatic adiposity, and overall fat mass, highlighting a central role for BCHE in...
A color-coded atlas of the cortical surface anatomy of the equine brain. The horse has a highly gyrencephalic cerebrum; however, detailed references of equine cortical surface anatomy remain limited, and terminology inconsistencies continue to complicate comparative, clinical, and educational applications. This research focused on creating a standardized, high-resolution, color-coded atlas of equine brain sulci and gyri, based on the Nomina Anatomica Veterinaria (NAV). Unassigned: Brains from ten neurologically normal horses were examined. Following fixation and dissection, the sulci and gyri of the lateral, dorsal, rostral, and medial surfaces were documented, col...
First-time description of MHC diversity in Straight Egyptian Arabian horses using immune-linked microsatellite markers. Understanding the immunogenetic structure of the major histocompatibility complex (MHC) in Arabian horses helps to clarify the population health structure-related phenotypes of this historically influential breed. Straight Egyptian (SE) Arabian horses, which fall under , are of global value but are limited in number, raising concerns about their distribution and the genetic diversity associated with immunity. In this study, we examined 57 Egyptian Arabian horses from two breeding programs: one in Germany (AR-DE) and the other in Qatar (AR-QA). Sixteen polymorphic intra-MHC microsatellite marke...
Factors affecting veterinary perception of the prevalence of back pain and dysfunction in dogs and horses. The prevalence of back pain in humans has been extensively reported, but in dogs it is unknown. In horses, research on the prevalence of back pain exists, but with reports ranging from 1% to 94%, indicating a data deficiency. Unassigned: Veterinarians were surveyed about their perception of the prevalence of back pain and dysfunction in dogs and horses for 6 different clinical scenarios. The respondents' education background and physical examination techniques were recorded. Unassigned: Perceptions of the prevalence of back pain and dysfunction varied widely both within and between education g...
Leishmania spp. infection in horses in an endemic area of canine leishmaniosis (north-western Algeria). Leishmaniosis is a parasitic disease caused by obligate intracellular protozoa of the genus Leishmania. It affects humans, canids, and rodents in endemic areas and is transmitted through the bites of infected sand flies. This study aimed to estimate the prevalence of equine leishmaniosis in horses from Tiaret Province, northwestern Algeria, an area endemic for canine leishmaniosis, and to identify the main risk factors associated with infection. The study included 75 local Barb horses and employed molecular and serological techniques for the detection of Leishmania infection. Among the sampled...
