Omneity® Pellets
All-In-One Vitamin & Mineral Pellet
Topic:Disease Diagnosis
Disease diagnosis in horses involves the identification and characterization of illnesses through various diagnostic methods and tools. This process is essential for effective veterinary care and management of equine health. Techniques used in diagnosing diseases in horses include clinical examinations, laboratory tests, imaging modalities such as ultrasonography and radiography, and molecular diagnostics. Blood tests are frequently utilized to assess parameters such as complete blood count and biochemical profiles, which can indicate underlying health issues. Additionally, advancements in genetic testing and biomarker identification have enhanced the ability to detect specific diseases early. This page aggregates peer-reviewed research studies and scholarly articles that explore diagnostic methodologies, their applications, and their impact on equine health management.
Keratomycosis in six horses in the United Kingdom. Six horses with keratomycosis were examined and three different clinical expressions of the disease were recognised. The diagnostic work-up and response to treatment is described.
Limitations to veterinary applications of new technologies in treatment and diagnostics. Technology promises to improve the lifestyle and life quality of humankind. As a rule, wherever human medicine goes, veterinary medicine is sure to follow. Nevertheless, the promise of technologic advances does not shine as bright for veterinarians as for human physicians. This trend is echoed in the business of animal health as pharmaceutic company after pharmaceutic company spins off or otherwise eliminates their animal health division. Instead, a small group of strictly animal health-oriented companies compete for the animal health dollar, promising that fewer and fewer expensive technologi...
Frontier medicine: the future and integrative medicine. Vigorous and prolonged effort is required to gain true mastery of the healing arts. Conventional and complementary medicine have complementary strengths and weaknesses. Like the yin and yang of traditional Chinese medicine, they naturally flow into one another by a process of induction, creating balance. Integrative medicine is the frontier; it is the future. If we are to progress beyond our current understanding and ability to heal, we must work with theoretic models that allow us and our perception to operate "outside the box." For some, this understanding is intuitive. It is through coopera...
The internet and the future of equine practice. The Internet is a fast-growing technology that allows information (text as well as images) to be shared across the world in an instant. As computer systems, software, and Internet services become less expensive options, the world becomes more experienced and comfortable with these technologies. In the foreseeable future, equine veterinarians are likely to be better educated about diseases and treatments, better connected with specialists for consultations on difficult cases, able to respond to client communications more efficiently, able to accurately educate clients, and able to communicate w...
Prospects for whole genome linkage disequilibrium mapping in thoroughbreds. Linkage disequilibrium (LD) mapping is often used in searches for genes governing economically significant traits and diseases. The D' coefficient is a commonly used measure of the extent of LD between all possible pairs of alleles at two markers. This study aimed to test the utility of the D' coefficient for LD mapping of a trait in a thoroughbred population. Microsatellite genotype data and grey coat colour as a trait model in a thoroughbred population were used to assess the extent of LD. We demonstrated that LD mapping was a reasonable approach for initial genome-wide scans in a thoroughbr...
An in vitro biomechanical comparison of the insertion variables and pullout mechanical properties of ao 6.5-mm standard cancellous and 7.3-mm self-tapping, cannulated bone screws in foal femoral bone. To compare screw insertion variables and pullout mechanical properties between AO 6.5-mm cancellous and 7.3-mm cannulated bone screws in foal femoral bone. Methods: A paired, in vitro mechanical study. Methods: Seven pairs of femora from immature (1-7 months) foals. Methods: The 6.5 cancellous and 7.3-mm cannulated screws were inserted at standardized proximal and distal metaphyseal, and mid-diaphyseal locations. Insertion torque, force, and time to drill, tap (6.5-mm cancellous), guide wire insertion (7.3-mm cannulated), and screw insertion were measured. Screw pullout properties (yield and f...
Genetic clustering of Borna disease virus natural animal isolates, laboratory and vaccine strains strongly reflects their regional geographical origin. The aim of this study was to gain more detailed insights into the genetic evolution and variability of Borna disease virus (BDV). Phylogenetic analyses were performed on field viruses originating from naturally infected animals, the BDV vaccine strain 'Dessau', four widely used laboratory strains and the novel BDV subtype No/98. Four regions of the BDV genome were analysed: the complete p40, p10 and p24 genes and the 5'-untranslated region of the X/P transcript. BDV isolates from the same geographical area exhibited a clearly higher degree of identity to each other than to BDV isolates from ot...
Determination of the stiffness of the equine spine. Mechanical properties of the equine back are the bases for realistic modelling of the back, which is recognised as an important step towards improved understanding of the pathogenesis of equine back conditions. Objective: The stiffness of the equine back depends on the direction of the applied force and on the position of the spine. Methods: Fourteen dissected spines were tested in a tensile testing machine. In 3 different positions, simulating dorsoventral, laterolateral and dorsoventral-30 degree rotated movement, force was applied on the dorsal spinous process of T12 to reach an excursion o...
[A horse with a badly-healing wound? A case report]. A 14-year-old Haflinger mare was presented with a wound on the right metatarsus which it had sustained 3 years earlier. The wound had never completely healed but had only recently become a problem. Over a period of several months, the wound became larger, produced a lot of exudate, and the horse became lame on the affected limb. Clinical examination and radiographs failed to reveal the cause of the deterioration. Histological evaluation of tissue removed during debridement of the wound revealed squamous cell carcinoma. Because the tumour had already invaded the bone, the prognosis was unfavour...
Metastatic tumors to the adrenal glands in domestic animals. Although metastases to the adrenals are common in humans, they have not been thoroughly studied in animals. The purpose of this retrospective study was to document the types of malignant tumors that metastasize to canine, feline, equine, and bovine adrenals, and the rate at which they do so. The average rate of adrenal involvement in metastatic cancer was 112/534 (21.0%) in dogs, 12/81 (14.8%) in cats, 18/67 (26.9%) in horses, and 5/16 (31.3%) in cattle. In dogs, 26 different tumor types metastasized to the adrenals. Pulmonary, mammary, prostatic, gastric, and pancreatic carcinomas, and melano...
The role of electromyography in clinical diagnosis of neuromuscular locomotor problems in the horse. Systematically performed EMG needle examination of muscles provides essential information about the functional aspects of the motor unit. However, clinical studies in which information is given on the diagnostic and discriminative values of electromyography (EMG) in the horse are scarce. Objective: To determine to what extent inclusion of EMG analysis in clinical examination contributes to determination of type and localisation of abnormality. Methods: EMG analysis, complete clinical examination and diagnosis of 108 horses (mean +/- s.d. age 75 +/- 3.8 years; bodyweight 548 +/- 86 kg; height 1...
Three-dimensional kinematic analysis of horses with induced tarsal synovitis. Techniques for 3D analysis have recently been developed. This study applied 3D kinematic analysis to describe the effects of lameness in the distal intertarsal (DIT) and tarsometatarsal (TMT) joints; complex joints such as this may show measurable amounts of movement outside the sagittal plane that may change in response to pathological conditions. Objective: Three-dimensional motions of the tarsal joint change after the induction of synovitis at the DIT and TMT joints. Methods: Twelve retroreflective markers, attached on the skin over the right limb, were used to develop 3D coordinate systems...
In vitro measurement of internal hoof strain. Strains during stance on the hoof wall surface have been measured by a number of authors in vitro and in vivo. Histological structure and mechanical properties vary through the wall thickness (radially); radial strain measurements may therefore aid the understanding of mechanical function of the capsule and adjacent tissues. Objective: To develop instrumentation capable of measuring internal hoof strain, and to carry out a preliminary comparison of normal and laminitic hooves. Methods: Six forelimbs from 4 horses, including 2 with laminitis from the same horse, were tested using an Instron tes...
Three-dimensional carpal kinematics of trotting horses. Descriptions of 3D kinematics assist in understanding joint function and dysfunction, and are an essential step toward 3D inverse dynamic analysis. Objective: To measure 3D carpal joint motion during trotting. Methods: Three-dimensional trajectories of bone-fixed markers on the radius and third metacarpus of the right forelimb of 3 healthy horses were recorded at 120 Hz using a 6-camera analysis system. Joint kinematics were calculated in terms of helical angles between the 2 segments using a spatial attitude method. Results: All horses showed carpal extension and internal rotation of the meta...
Detection of spinal ataxia in horses using fuzzy clustering of body position uncertainty. Subjective neurological evaluation in horses is prone to bias. An objective method of spinal ataxia detection is not subject to these limitations and could be of use in equine practice and research. Objective: Kinematic data in the walking horse can differentiate normal and spinal ataxic horses. Methods: Twelve normal and 12 spinal ataxic horses were evaluated by kinematic analysis walking on a treadmill. Each body position signal was reduced to a scalar measure of uncertainty then fuzzy clustered into normal or ataxic groups. Correct classification percentage (CCP) was then calculated using m...
Analysis of cartilage oligomeric matrix protein (COMP) degradation and synthesis in equine joint disease. Cartilage oligomeric matrix protein (COMP) is abundant within cartilage; its turnover and/or degradation have been investigated in various equine joint diseases and it has been suggested that COMP fragmentation might be useful for monitoring such conditions. Objective: To determine whether COMP metabolism is compromised in equine osteoarthritis (OA) and whether COMP degradation is a useful joint marker representing cartilage destruction. Objective: A monoclonal antibody (mAb) with a higher affinity for degraded COMP allows discrimination of diseased joints by quantifying COMP levels and fragme...
Biochemical markers of bone metabolism and risk of dorsal metacarpal disease in 2-year-old Thoroughbreds. Dorsal metacarpal disease (DMD) is a common problem in 2-year-old racehorses and results in loss of a significant number of days from training. Biochemical markers of bone cell activity measured early in the training season could have value for identifying 2-year-old Thoroughbred racehorses that develop DMD. Objective: To determine the association between serum concentrations of osteocalcin, the carboxyterminal propeptide of type I collagen (PICP) and the carboxyterminal cross-linked telopeptide of type I collagen (ICTP) measured early in the training season and the risk of DMD. Methods: Blood...
Immunoglobulin and peripheral B-lymphocyte concentrations in Fell pony foal syndrome. Fell pony foals are affected by a congenital fatal disease that leads to profound anaemia and immunodeficiency. Previous studies comparing healthy and affected foals have shown normal T-cell populations, but a severe B-lymphopenia. Objective: To measure the levels of individual immunoglobulin subisotypes in normal and affected Fell ponies and correlate these levels with the number of peripheral B-lymphocytes. Methods: Serum levels of individual immunoglobulin subisotypes were measured by ELISA and correlated with the number of peripheral B-lymphocytes (measured by flow cytometry). Results: Aff...
Preparation of recombinant African horse sickness virus VP7 antigen via a simple method and validation of a VP7-based indirect ELISA for the detection of group-specific IgG antibodies in horse sera. This paper describes the production and purification of a group-specific recombinant protein VP7 of African horse sickness virus serotype 3 (AHSV-3) and validation of an I-ELISA for the detection of IgG-antibodies to VP7 in horse sera. Baculovirus-expressed VP7 crystals were purified from infected insect cells. Analytical accuracy of the I-ELISA was examined using sera (n = 38) from an experimentally infected horse, from foals born to vaccinated mares, from guinea-pigs immunized with nine serotypes of AHSV, and from sera of animals infected with other orbiviruses. Compared to traditional serol...
The effect of bilateral glossopharyngeal nerve anaesthesia on swallowing in horses. Dysfunction of the glossopharyngeal nerve has been implicated as a cause of dysphagia in horses. However, recent studies have indicated that this is not the case. Objective: To determine whether bilateral glossopharyngeal nerve anaesthesia would cause dysphagia in horses or result in measurable alterations in the timing, function, or sequence of swallowing. Methods: Swallowing was evaluated in 6 normal horses with and without bilateral glossopharyngeal nerve anaesthesia. Swallowing dynamics were assessed subjectively and objectively based on time from prehension of food until swallowing, numbe...
Ultrastructural immunolocalisation of bone sialoprotein in the osteocartilagenous interface of the equine third carpal bone. One of the most common causes of lameness in racehorses is osteoarthritis (OA). Pathogenesis is not clear and pathological processes of the different joint tissues interact in often progressive events. The interface between cartilage and newly synthesised bone has been shown to be particularly enriched in bone sialoprotein (BSP), a cell-binding matrix protein. Objective: To establish whether changes in the concentration of BSP may serve as a marker for early biochemical changes of the subchondral bone. Methods: Articular cartilage, cartilage/bone interface and subchondral bone of the proximal ...
Relationship between synovial fluid levels of glycosaminoglycans, hydroxyproline and general MMP activity and the presence and severity of articular cartilage change on the proximal articular surface of P1. Osteoarthritis (OA) is one of the most prevalent and disabling chronic conditions affecting horses and leads to degeneration of articular cartilage. Diagnosis is based on clinical signs in combination with radiography, which is relatively insensitive and provides only an indication of accumulated damage. Alternative methods, such as molecular markers, are therefore needed that can quantitatively, reliably and sensitively detect osteoarthritic changes in the joints at an early stage of the disease. If such markers are to be used reliably, it is important to know the relationship between marker ...
Histology and ultrastructure of the equine lingual tonsil. I. Crypt epithelium and associated structures. The microstructural and ultrastructural features of the equine lingual tonsil were studied in five young horses. Located at the root of the tongue it presented an irregular surface with rounded elevations, numerous folds and crypts. Stratified squamous non-keratinized epithelium lining its outer surface was modified by heavy infiltration of lymphoid cells to form reticular epithelium within the crypt. The latter implies a role in initiating and maintaining immune responses to incoming infectious agents and antigens. Lamellated structures resembling Hassall's corpuscle were observed towards the...
Histomorphology of the equine cervix. The cervix provides an effective, morphological barrier between the uterus and the outside world. The aim of this study was to characterize the epithelial morphology and the vascular structures of the equine cervix along the longitudinal and horizontal axis in more detail by light microscopy. For this purpose, cervical tissue specimens that had been removed from five different regions along the caudocranial axis of 10 genitally healthy mares were available. The histological staining was carried out with haematoxylin-eosin, azan according to Heidenhain, periodic acid-Schiff reaction and resorci...
Semi-nested PCR for the specific detection of Habronema microstoma or Habronema muscae DNA in horse faeces. Habronema microstoma and Habronema muscae (Spirurida: Habronematidae) are parasitic nematodes which infect the stomach and/or skin of equids. The accurate diagnosis of gastric habronemosis is central to studying its epidemiology, but data on its distribution and prevalence are lacking, mainly due to the limitations of clinical and coprological diagnosis in live horses. To overcome this constraint, a two-step, semi-nested PCR-based assay was validated (utilizing genetic markers in the nuclear ribosomal DNA) for the specific amplification of H. microstoma or H. muscae DNA from the faeces from ho...
Unusual vascular ring anomaly in a foal. A 2.5-month-old filly was presented with signs of esophageal obstruction. The filly was euthanized and postmortem examination revealed a vascular ring anomaly. The vascular ring anomaly was not caused by a persistent right aortic arch, which is the only vascular ring anomaly reported to occur in horses. Une pouliche de 2 1/2 mois a été présentée avec des signes d’obstruction œsophagienne. Après euthanasie, l’examen a révélé une malformation vasculaire annulaire. L’anomalie vasculaire annulaire n’était pas due à la persistance de l’arc aortique droit, seule malformation va...
Primary desmitis of the palmar and plantar annular ligaments in horses: 25 cases (1990-2003). To determine clinical and ultrasonographic abnormalities in horses with primary desmitis of the palmar or plantar annular ligament (PAL) and the response to treatment. Methods: Retrospective study. Methods: 25 horses. Methods: Data collected from medical records included signalment, horse use, affected limb, severity and duration of lameness, results of diagnostic tests performed, ultrasonographic findings, treatment, and outcome. Results: All horses had a prominent swelling in the region of the affected PAL, and signs of pain were evident during palpation of the swelling. In all horses, the a...
