Analyze Diet

Topic:Equine Science

Equine Science encompasses the study of horses and their management, health, and performance. This field integrates various scientific disciplines such as biology, genetics, nutrition, physiology, and veterinary medicine to understand and improve the well-being and capabilities of horses. Areas of focus include equine anatomy, reproduction, behavior, and disease prevention. Research in equine science aims to enhance horse care, optimize training and performance, and address health challenges. This page compiles peer-reviewed research studies and scholarly articles that explore the diverse aspects of equine science, providing insights into the latest advancements and methodologies in the field.
Size of the C7-T1 articular processes is overestimated on lateral radiographs when compared to standing cone beam computed tomography.
Equine veterinary journal    May 13, 2026   doi: 10.1002/evj.70184
Cianci JM, Bills KW, Skelton G, Stewart HL, Johnson AL, Colmer SF, Stefanovski D, Brown KA.Radiography of the caudal cervical vertebral column is commonly performed in horses, but image distortion may occur due to factors such as scapular superimposition, divergent beam, and the distance between anatomy and detector. These variables can falsely suggest enlargement of the articular processes (APs) at the seventh cervical and first thoracic vertebrae (C7-T1). Objective: To assess the presence of magnification of C7-T1 APs as determined by AP:vertebral body (VB) height ratio on lateral radiographs compared to cone beam computed tomography (CBCT) as a gold standard, and to explore inter...
Evaluating hormonal levels in plasma and saliva as temperament indicators in horses.
Scientific reports    May 12, 2026   doi: 10.1038/s41598-026-51061-5
Choi Y, Jung Y, Wickens CL, Park Y, Yoon M.Assessing the temperament of horses is important to ensure their welfare, management, and safety. This study evaluated the plasma-saliva relationships for cortisol, oxytocin, and serotonin, and explored their associations with temperament traits in 40 horses using enzyme-linked immunosorbent assays. Significant correlations were observed between cortisol (r = 0.441) and oxytocin (r = 0.587) concentrations, whereas serotonin showed a weak correlation (r = 0.133). Bland-Altman analysis revealed acceptable agreement for cortisol but substantial individual variability for oxytocin, despite its str...
Vaginal host-microbe signatures linked to placental outcomes in mares.
Equine veterinary journal    May 10, 2026   doi: 10.1002/evj.70185
van Heule M, Heil B, Norris JK, Gedye K, Lin X, De Spiegelaere W, Daels P, Dini P.Ascending placentitis is a leading cause of late-term pregnancy loss in mares. Although pathogens are presumed to ascend from the caudal reproductive tract, the association between the vaginal microbiome and placentitis has not been systematically examined. Objective: To characterise microbial and host gene expression in the equine vagina during gestation and to identify taxa or transcripts that were associated with the presence of an abnormal placenta at birth. Methods: Prospective, paired observational study. Methods: Vaginal wall swabs were collected from 49 multiparous Thoroughbred mares a...
Lacrimal caruncula thermography overestimates rectal temperature in horses: Influence of camera type and measurement distance.
Equine veterinary journal    May 9, 2026   doi: 10.1002/evj.70174
Orhun ÖT, Turgut F, Okur S, Arslan T, Yanmaz LE.Rectal temperature (RT) is the gold standard for assessing body temperature in horses, but handling and welfare concerns limit its use. Thermography of the lacrimal caruncula (LC) has been proposed as a noncontact alternative, although its accuracy may be influenced by device characteristics and measurement distance. Objective: To evaluate agreement between LC thermography and RT in horses, compare two infrared thermal cameras and determine the effect of camera-to-subject distance on measurement bias and variability. Methods: LC temperature was recorded at 0.5, 1 and 2 using two cameras (IR Fl...
Long-Read Sequencing for Species-Level Resolution of the Equine Gut Microbiota Reveals the Need for Improved Databases.
Animals : an open access journal from MDPI    May 9, 2026   Volume 16, Issue 10 1459 doi: 10.3390/ani16101459
Leduc L, Boucher L, Mach N, Leclère M, Costa M.Differences in gut microbiota composition related to diet have been reported in horses, but characterization of specific microbial taxa remains limited, particularly at the species level. The objective of this study was to use long-read sequencing of the 16S rRNA gene to provide additional taxonomic insight into the intestinal microbiota in horses. Fecal samples were collected from 12 horses on pasture and from 6 of them after switching to a hay diet. Sequencing yielded low read counts per sample, and the analysis failed to detect statistical differences in alpha- and beta-diversity among diet...
Welfare Assessment in Equine-Assisted Service (EAS) Horses.
Animals : an open access journal from MDPI    May 8, 2026   Volume 16, Issue 10 1434 doi: 10.3390/ani16101434
Carvalho Seabra J, Hess T, Finnestead T, Grandin T.Equine-Assisted Service (EAS) horses can be exposed to stressors that affect their behavior and welfare. Previous studies investigated behavioral and physiological indicators (PI) only during therapy sessions. This study evaluated the frequency of stress and pain behaviors (FSPB) in horses with different riders, while assessing welfare through housing, management, and PI. Ten horses were observed during EAS sessions with three rider groups: able-bodied, clients with significant physical impairment, and clients with no significant physical impairment, totaling twenty-six riders. Behavioral data...
The effects of citrulline on horse milk components and fecal metabolism were analyzed based on LC-MS and 16s amplicon.
BMC veterinary research    May 7, 2026   doi: 10.1186/s12917-026-05534-4
Ma Y, Liu P, Li X, Hu Q, Lin J, Yang F, Yang K.The lactation period in mares is critical, as it significantly influences foal health and subsequent reproductive outcomes. Developing a precise nutritional strategy for lactating mares is a primary research focus. L-citrulline, a bioactivenon-protein amino acid and precursor to arginine, has been shown to enhance mammary blood flow and reduce oxidative stress, yielding promising results in livestock lactation studies. However, research on its effects in Equus species remains limited. There is a notable gap in data regarding its impact on lactation performance blood biochemistry, and its role ...
Sensitive and selective quantification of elcatonin in equine plasma and urine using LC-FAIMS-MS/MS.
Journal of pharmaceutical and biomedical analysis    May 7, 2026   Volume 279 117551 doi: 10.1016/j.jpba.2026.117551
Ohnuma K, Fukazawa M, Uchida T, Kato T, Sugai-Bannai M, Shibuya M, Yamada M, Hirano-Kodaira M.The use of drugs for enhancing bone strength is prohibited under international regulations governing horse racing and equestrian sports. Elcatonin, a synthetic peptide with analgesic and bone-strengthening properties, is routinely monitored for compliance. However, its cyclic structure and high molecular weight hinder reliable detection. In this study, we developed and validated a sensitive liquid chromatography-high-field asymmetric waveform ion mobility spectrometry-tandem mass spectrometry method for quantifying elcatonin in equine plasma and urine. Sample preparation was optimised via weak...
Factors influencing gestation length in Mares in the Tiaret region, Algeria.
Journal of equine veterinary science    May 7, 2026   Volume 163 105925 doi: 10.1016/j.jevs.2026.105925
Boubakeur E, Belmdjahed M.Gestation length is a key parameter in equine reproductive management, yet its variability remains poorly documented in Barb and Arabian mares under Algerian conditions. Objective: This study aims to determine gestation length in mares and to assess the influence of various factors, including breed, year of mating, mating month, mare age, parity, reproductive status, stallion and the foal's sex on gestation duration. Methods: A total of 133 mares from the National Stud Farm of Tiaret (Algeria) were monitored over the period 2021-2024. Results: The mean gestation length was 332.5 days (range: 3...
Telomere-to-telomere Genome Assembly of Equus kiang (Tibetan wild donkey).
Scientific data    May 6, 2026   doi: 10.1038/s41597-026-07355-4
Zheng Y, Hu Y, Suo J, Wang W, Wang Y, Cao L, Sun P, Zhang M, Zhao M, Yang W, Li X, Jiang B, Bu X, Du X, Zhang Q.The Tibetan wild donkey (Equus kiang) is an endemic equid species inhabiting the Qinghai-Tibet Plateau, exhibiting remarkable chromosomal diversity within the Equus genus. Here we present a chromosome-level, telomere-to-telomere genome assembly for E. kiang generated using Illumina short reads, PacBio HiFi long reads, and Hi-C sequencing data. The final assembly spans 4.02 Gb and comprises 27 pseudochromosomes. Specifically, 8 chromosomes are gapless, 8 chromosomes have one gap, 7 chromosomes have 2-4 gaps, and the remaining 4 chromosomes have five or more gaps. Benchmarking Universal Single...
Quantitative evaluation of behavioral and physiological adaptation during retraining of retired Thoroughbred racehorses.
Journal of equine veterinary science    May 4, 2026   Volume 163 105920 doi: 10.1016/j.jevs.2026.105920
An SJ, Lee JH, Lee HY, Kim BS, Forbes E, Ryu SH.Retired Thoroughbred racehorses commonly transition into riding roles, yet outward behavioral calmness may not fully reflect internal physiological regulation, with implications for safety. Objective: To quantify behavioral and autonomic adaptation across repeated startle exposures and assess concordance between behavioral and physiological indicators. Methods: Twelve retired Thoroughbreds underwent standardized plastic-bag startle trials conducted across three sessions (T1-T3), each including pre-training (Before) and post-training (After) phases, with three repeated exposures at 5-minute int...
Accuracy of large language model-based artificial intelligence tools for equine topics.
Journal of equine veterinary science    May 1, 2026   105924 doi: 10.1016/j.jevs.2026.105924
Aldworth-Yang S, Coleman SJ, O'Reilly K, Catalano DN.Artificial intelligence (AI) platforms are becoming increasingly popular as resources for equine information. However, these platforms generate responses from a wide range of sources and do not always distinguish between fact and opinion. Objective: The objective of this study was to assess the accuracy and quality of AI-generated answers to equine-related questions. Researchers hypothesized that AI platforms could answer basic equine questions effectively but would perform poorly on complex topics or questions. Methods: Forty questions were written covering general horse care, facilities mana...
Beyond cortisol: evaluating serotonin, brain-derived neurotrophic factor, and oxytocin as indicators of equine welfare across three training regimens.
BMC veterinary research    April 30, 2026   doi: 10.1186/s12917-026-05507-7
Kacprzyk M, Tobolski D, Kiełbik P, Grzędzicka-Agko J, Milczek-Haduch D, Stefanik E, Dąbrowska I, Pawliński B, Gołębiewski M....BACKGROUND: Quantifying the balance between workload, stress, and recovery across equine disciplines remains challenging, and no single circulating biomarker provides a definitive index of welfare-related stress load. Cortisol, oxytocin, serotonin (5-hydroxytryptamine, 5-HT), and brain-derived neurotrophic factor (BDNF) may provide complementary information on endocrine and neuromodulatory responses. METHODS: Paired serum samples were collected from show jumping horses (n = 17) before and after competition, racehorses (n = 43 enrolled, n = 41 analyzed) before and after sessions at ...
The Effects of L-Citrulline on the Apparent Digestion and Metabolism of Nutrients, Blood Hormone Levels, Amino Acid Metabolism and the Diversity of Faecal Microbiota in Mares in the Later Stage of Pregnancy.
Life (Basel, Switzerland)    April 30, 2026   Volume 16, Issue 5 744 doi: 10.3390/life16050744
Liu P, Yang F, Li J, Li C, Guo X, Li X.This study aimed to investigate the impact of dietary L-citrulline supplementation on the health of mares during late gestation. Thirty-two healthy mares in late pregnancy were randomly assigned to four groups: a control group (CON, 0 g/d) and three treatment groups receiving 15, 30, and 45 g/d/head of L-citrulline, respectively. The trial spanned 72 days, including a 12-day adaptation phase and a 60-day formal feeding period. A fixed daily feeding amount of 11.2 kg/head was provided, ensuring complete consumption and consistent dry matter intake across all groups. Results demonstrated that su...
Clinical and Biochemical Effects of Intra-Articular Autologous Conditioned Serum and Triamcinolone in an Equine Model of Synovitis.
Animals : an open access journal from MDPI    April 29, 2026   Volume 16, Issue 9 1371 doi: 10.3390/ani16091371
Alvarez AV, Wooldridge A, Caldwell F, Zetterström S, Menarim BC, Towns TJ, Graff EC, Boone L.Synovitis is a key contributor to the development of OA, and early modulation of the synovial environment may help limit downstream cartilage damage. This study compared the clinical and biochemical effects of intra-articular triamcinolone acetonide (TA) and autologous conditioned serum (ACS) in an equine model of IL-1β-induced synovitis. Six healthy adult horses were used in a crossover design involving five groups: PBS (negative control), IL-1β (positive control), IL-1β + ACS, IL-1β + TA, and an exploratory ACS-alone group administered post hoc to isolate its effects without IL-1β inter...
Rostral hard palate swelling (lampas) in horses: true or fiction?
Journal of equine veterinary science    April 29, 2026   105917 doi: 10.1016/j.jevs.2026.105917
Pereira LMA, Schade J, Pereira LMA, Tocheto R, Cristo TG, Casagrande RA, Souza AF, Fonteque JH.This study aimed to determine the occurrence of swellings (lampas) in the rostral portion of the hard palate in young, adult, and elderly horses and to characterize them histologically. One hundred and twenty horses of different ages were evaluated by oral inspection. Horses were classified with lampas when the mucosa of the hard palate extended beyond the occlusal surface of the upper incisors and samples of palatal mucosa were collected. Ten other horses without rostral hard palate alterations, euthanized for unrelated causes, were included as the control group. Histological analysis include...
Machine Learning-Based Genome-Wide Association Study Reveals Genetic Loci Associated with Body Measurement Traits in Yili Horses.
Animals : an open access journal from MDPI    April 29, 2026   Volume 16, Issue 9 1373 doi: 10.3390/ani16091373
Shen Z, Yang L, Xue Y, Chang X, Shen J, Sun W, Zeng Y, Meng J, Yao X.Body measurement traits are key indicators for evaluating growth performance, production potential, and breeding value in Yili horses. However, studies investigating the association between body measurement traits and mutation loci in Yili horses remain limited. In this study, 255 adult Yili mares were used as the study population, including 152 speed-type and 103 meat-type individuals. Whole-genome resequencing was performed, and four phenotypic traits and body weight were measured. A mixed linear model (MLM)-based genome-wide association study (GWAS) was conducted using GEMMA (v 0.98.5), inc...
Measuring Chuckwagon Racehorse Movement Asymmetry Before and After Racing Using Wearable GNSS-IMUs: A Preliminary Study.
Animals : an open access journal from MDPI    April 29, 2026   Volume 16, Issue 9 1361 doi: 10.3390/ani16091361
Eamon CM, van den Broek M, Garcia-Alamo K, De Bruyne C, Davis BL, Fallscheer M, Frostad S, Pajor E, Skotarek Loch S, Weller R, Chan ZYS, Pfau T.In Chuckwagon racing, teams of four Thoroughbred horses pull wagons at high speeds. Movement symmetry is a key locomotion metric linked to force production, racing direction, and lameness. Racehorse symmetry in trot during on-track warmups and cooldowns was assessed. Over 10 days, 60 horses (average 8 per day) were fitted with Global Navigation Satellite Systems combined with Inertial Measurement Unit (GNSS-IMU) sensors. Weight-bearing asymmetry was quantified using the minimum difference (MnD) in vertical trunk displacement between diagonal limb pairs, and push-off asymmetry was quantified us...
Monitoring horse behaviour with deep learning models.
The veterinary quarterly    April 28, 2026   Volume 46, Issue 1 2665442 doi: 10.1080/01652176.2026.2665442
Giannone C, Maccario C, Dalla Costa E, Atallah E, Bovo M.Detailed analysis of stabled horse behaviour can reveal accurate information about its well-being. Advances in deep learning now allow these behaviours to be tracked without being invasive through the use of video data. This study evaluated a convolutional neural network for recognising standing, lying, and drinking behaviours in a horse housed in a wooden stall and recorded continuously over 29 consecutive days. Model predictions were compared with manually annotated ground truth data. Standing was detected with high precision (97.5%) and high recall (89.2%). Lying behaviour was classified wi...
Review of articular cartilage repair techniques and their application in the horse.
Equine veterinary journal    April 28, 2026   doi: 10.1002/evj.70162
Barton CK, Nelson BB, Goodrich LR.Articular cartilage lesions represent a significant career-limiting problem in athletic horses. A healthy articular cartilage surface is vital for optimal joint function, and defects can result in irreversible degenerative changes. Successful treatment of cartilage lesions remains a long-standing challenge for orthopaedic surgeons, prompting ongoing research into new surgical techniques for their management. This narrative review describes surgical procedures for the treatment of cartilage/osteochondral lesions, as well as the use of the horse as a highly translational preclinical model to hum...
Validation of a stall-side immunoglobulin assay for use in equine reproductive management.
Equine veterinary journal    April 28, 2026   doi: 10.1002/evj.70172
Moore L, McLain A, McDowell SH, Ledgerwood CJ, Bailie E, Nesbit MA, Moore T.Equine foals receive IgG from mare colostrum through passive transfer. Failure of passive transfer (FPT) is a significant risk to the foal's life, leaving them vulnerable to infection and sepsis. Radial Immunodiffusion (RID) and immunoturbidimetric assays quantify IgG present in a foal sample but require a laboratory to complete. Accurate, reproducible, stall-side testing to rapidly quantify IgG would allow for expedited clinical decisions, with potential to improve equine foal care and survival. Objective: To evaluate the analytical and clinical performance of a stall-side IgG lateral-flow te...
Pasture Intake of Whole-Day Stocked Thoroughbred Foals With Growth.
Animal science journal = Nihon chikusan Gakkaiho    April 28, 2026   Volume 97, Issue 1 e70168 doi: 10.1111/asj.70168
Tanabe T, Matsui A, Tominari M, Ueda K, Mitani T, Kawai M.We investigated the changes in the forage and nutrient intake of thoroughbred foals on pastures with whole-day stocking with age. Twelve thoroughbred foals were used and were allowed to graze on a pasture for 21 h/day from June to September. All foals were weaned just before 5 months of age. The pasture intake of the foals was measured using the double-indicator method. During the suckling period, the total voluntary dry matter intake (VDMI) of the pasture for foals was higher at 4 months (2.1 kgDM/day) than at 2 months (1.3 kgDM/day). However, VDMI of pastures per body weight (%BW) for foal...
Is managing horses ‘as wild’ beneficial for dung beetle assemblages and their associated ecological functions?
Journal of environmental management    April 28, 2026   Volume 406 129756 doi: 10.1016/j.jenvman.2026.129756
Mutillod C, Perrin W, Buisson É, Tatin L, Mesléard F, Jay-Robert P, Dutoit T.In Europe, where livestock has largely replaced wild ungulates over the centuries, dung beetle assemblages have become largely dependent on livestock farming. The radical changes in livestock farming practices during the 20th century have therefore had a profound impact on these assemblages. Trophic rewilding with large herbivores is an important focus in European rewilding projects, and dung beetle assemblages appear to be good indicators of the success of rewilding. By comparing the composition of dung beetle assemblages and the rate of dung disappearance in three neighboring grassland habit...
Comparison Between Inertial Sensor and Video-Based Detection of Spatiotemporal Limb Movement Parameters During Equine Swimming.
Sensors (Basel, Switzerland)    April 28, 2026   Volume 26, Issue 9 2743 doi: 10.3390/s26092743
Marin F, Giraudet C, Gaulmin P, Moiroud C, De Azevedo E, Hatrisse C, Ben Mansour K, Martin P, Audigie F, Chateau H.Equine swimming is increasingly used for injury prevention and rehabilitation, but objective analysis of movement during swimming remains limited compared to land-based locomotion. Spatiotemporal parameters are essential for evaluating therapeutic outcomes, yet capturing these parameters is technically challenging due to difficulties in observing limb motion in water. Inertial sensors, already widely applied in equine science, offer a promising solution for measuring swimming kinematics objectively. The objective of this study was to evaluate the reliability of inertial sensors placed on equin...
A Familial X-Linked Disorder of Sexual Development in Thoroughbred Horses Associated With a Novel Androgen Receptor Splice-Site Variant.
Animal genetics    April 27, 2026   Volume 57, Issue 3 e70112 doi: 10.1002/age.70112
Letko A, Jagannathan V, Drögemüller C, Lühken G.No abstract available
Piezosurgical partial ostectomy of the incisive bone for an ossifying fibroma removal in a 4-year-old Warmblood gelding.
Equine veterinary journal    April 26, 2026   doi: 10.1002/evj.70178
Forni G, Falomo ME, Casalini I, Isola M.Ossifying fibromas are uncommon in horses and complete surgical excision with premaxillectomy, maxillectomy or mandibulectomy is recommended. Piezosurgery has been previously used in equines only in one study. Objective: To report a case of ossifying fibroma treated with piezosurgery and to describe its follow-up. Methods: Clinical case report. Methods: A 4-year-old Zangersheide gelding was presented for a mass occupying the space of tooth 202. Histology confirmed the diagnosis of ossifying fibroma, which was removed by resection of soft tissues and a slice of the premaxilla with a piezotome. ...
Ertugliflozin decreases the insulin spike in non-insulin dysregulated standardbred horses following intra-articular triamcinolone administration.
Domestic animal endocrinology    April 26, 2026   Volume 96 107016 doi: 10.1016/j.domaniend.2026.107016
Darch C, Hyndman TH, Byrne D, Rendle DI, Fraser B.Horses with equine metabolic syndrome (EMS) are at an increased risk of laminitis following corticosteroid use. Ertugliflozin reduces hyperinsulinemia in horses with EMS, and therefore may be a management option to reduce the insulin spike seen post corticosteroid use. It was hypothesised that oral ertugliflozin, administered before and after intra-articular (IA) triamcinolone acetonide (TA), will significantly lower the insulin response in comparison to placebo. A randomised, blinded, placebo-controlled, crossover design was performed on eight adult standardbred geldings with no history of la...
Assessment of acute and chronic pain in working horses and donkeys in Egypt and the influence of observer training.
Journal of equine veterinary science    April 24, 2026   105915 doi: 10.1016/j.jevs.2026.105915
Lap A, Gudden DDM, Lashley MJJO, van Loon JPAM, Naoum E.Pain assessment can support monitoring welfare in working equids. Objective: To assess agreement of structured pain scores before and after training observers and to assess differences in pain scores between working equids with or without acute or chronic pain and to compare them to non-working equids. Methods: In part 1, eleven veterinarians and nine technicians performed pain scores before and after training. Agreement with reference scores was analyzed with Intra Class Correlation (ICC) analysis. In part 2, pain assessments were performed in 96 donkeys (n=40 fit-to-work, n=21 acute pain, n=...
Efficacy of an iron absorption test on assessing iron status in horses.
Journal of equine veterinary science    April 23, 2026   105913 doi: 10.1016/j.jevs.2026.105913
Gluck-Flynn CR, Pratt-Phillips SE.Iron absorption tests (IATs) are used to evaluate iron absorption in humans. However, this testing has not been conducted in horses. Objective: The objective was to assess how a large dose of iron affects blood iron parameters during an IAT. Methods: Mixed-breed geldings (n=12) were offered 2% of their bodyweight (BW) in mixed-grass hay and an iron-free vitamin-mineral supplement. A baseline IAT (Baseline IAT) was performed, where horses received 25 milligrams per kilogram (mg/kg) of BW of iron (ferrous sulfate) orally. Jugular venous samples were taken before the iron dose, and 3-, 6-, 12-, a...
Application of 3D scanning for modeling and morphometric assessment of Mangalarga Marchador Horses.
Journal of equine veterinary science    April 23, 2026   105907 doi: 10.1016/j.jevs.2026.105907
E Alves TTB, Teixeira CS, de Lana DS, Sancler-Silva YFR, Dallago GM, de Godoi FN, Schultz EB.Morphometric traits are essential for assessing conformation, locomotion, and selection in equine breeding programs. However, manual measurements are laborious, require physical contact, and may cause stress to animals, highlighting the need for alternative technologies. Objective: This study evaluated the use of a portable 3D scanner for three-dimensional modeling and morphometric assessment of Mangalarga Marchador horses. Methods: Fifty-two adult horses were measured using manual and 3D scanning methods to assess ten morphometric traits: withers height (WH), hip height (HH), head length (HL)...
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