Equine Science encompasses the study of horses and their management, health, and performance. This field integrates various scientific disciplines such as biology, genetics, nutrition, physiology, and veterinary medicine to understand and improve the well-being and capabilities of horses. Areas of focus include equine anatomy, reproduction, behavior, and disease prevention. Research in equine science aims to enhance horse care, optimize training and performance, and address health challenges. This page compiles peer-reviewed research studies and scholarly articles that explore the diverse aspects of equine science, providing insights into the latest advancements and methodologies in the field.
Dell A, Curry M, Yarnell K, Starbuck G, Wilson PB.The Cleveland Bay horse is one of the oldest equines in the United Kingdom, with pedigree data going back almost 300 years. The studbook is essentially closed and because of this, there are concerns about loss of genetic variation across generations. The breed is one of five equine breeds listed as "critical" (<300 registered adult breeding females) by the UK Rare Breeds Survival Trust in their annual Watchlist. Due to their critically endangered status, the current breadth of their genetic diversity is of concern, and assessment of this can lead to improved breed management strategies. Herein...
Thompson RE, Johnson AK, Dini P, Turco MY, Prado TM, Premanandan C, Burton GJ, Ball BA, Whitlock BK, Pukazhenthi BS.The endometrium, the inner uterine lining, is composed of cell layers that come in direct contact with an embryo during early pregnancy and later with the fetal placenta. The endometrium is responsible for signals associated with normal reproductive cyclicity as well as maintenance of pregnancy. In the mare, functionally competent in vitro models of the endometrium have not been successful. Furthermore, the ability to study various reproductive processes in vitro may allow critical evaluation of signaling pathways involved in the reproductive diseases of animals that cannot be handled frequent...
Valberg SJ, Finno CJ, Henry ML, Schott M, Velez-Irizarry D, Peng S, McKenzie EC, Petersen JL.Commercial genetic tests for type 2 polysaccharide storage myopathy (PSSM2) and myofibrillar myopathy (MFM) have not been validated by peer-review, and formal regulation of veterinary genetic testing is lacking. Objective: To compare genotype and allele frequencies of commercial test variants (P variants) in MYOT (P2; rs1138656462), FLNC (P3a; rs1139799323), FLNC (P3b; rs1142918816) and MYOZ3 (P4; rs1142544043) between Warmblood (WB) and Arabian (AR) horses diagnosed with PSSM2/MFM by muscle histopathology, and phenotyped breed-matched controls. To quantify variant frequency in public reposi...
Rodrigues JB, Sullivan RJE, Judge A, Norris SL, Burden FA.Across Asia the brick-kiln industry is expanding. In Nepal, urban dwelling has increased in recent years, raising requirement for low-cost, mass produced bricks to meet the population needs. Working equids (WEs) play a key role in non-mechanised kilns. Assessing the welfare of these equids is the starting point to addressing concerns. In line with One Welfare principles, the health and welfare of animals, people and the kiln environment are interlinked. In December 2019, 119 WEs were assessed in seven brick kilns in three districts of Nepal, using the Equid Assessment Research and Scoping tool...
Shakeshaft A, Tabor G.Dynamic mobilisation exercises (DME) are often used as part of a physiotherapy rehabilitation programme. Whilst immediate kinematic effects have been measured, the change in posture is anecdotally reported to have a longer duration. This study aimed to test the reliability of a simple objective measurement method, suitable for use in clinical practice, and to objectively measure equine thoracolumbar posture, before and after DME. A single investigator took triplicate measurements of the sagittal thoracolumbar shape using a flexicurve ruler (FCR) then triplicate measurements of the thoracolumba...
Fenner K, Matlock S, Williams J, Wilson B, McLean A, Serpell J, McGreevy P.The Equine Behaviour Assessment and Research Questionnaire (E-BARQ) was developed to obtain quantitative data on the domestic equine triad: training, management and behaviour. It can be taken repeatedly, thus collecting longitudinal data to enable evaluation of how changes in a horse's training and management are reflected in that horse's behaviour over time and how these changes can impact horse welfare in the longer term. Questionnaire validation and reliability were tested by determining (a) whether an owner's subjective ratings of their horse's problematic behaviours or undesirable tempera...
Gillon A, Ho ENM, Chan GHM, Kauff A, Hughes G, Lund RA, Ashley Z, Wan TSM, Heather AK.Altrenogest is a commonly used progestogen for the suppression of oestrus and associated distracting behaviours that interfere with training and performance of female racehorses. The steroid is derived from 19-nor testosterone and is structurally similar to the anabolic androgenic steroid, trenbolone. In this study, the relative androgen potency of altrenogest was determined by a kidney (HEK293) cell androgen bioassay. The HEK293 bioassay shows that in its pure form, altrenogest has a high relative potency compared with testosterone but is not as strong as β-trenbolone. Our results also show ...
Marin F.Motion is key to health and wellbeing, something we are particularly aware of in times of lockdowns and restrictions on movement. Considering the motion of humans and animals as a biomarker of the performance of the neuro-musculoskeletal system, its analysis covers a large array of research fields, such as sports, equine science and clinical applications, but also innovative methods and workplace analysis. In this Special Issue of Sensors, we focused on human and animal motion-tracking using inertial sensors. Ten research and two review papers, mainly on human movement, but also on the locomot...
Dorado J, Bottrel M, Ortiz I, Díaz-Jiménez M, Pereira B, Consuegra C, Carrasco JJ, Gómez-Arrones V, Domingo A, Hidalgo M.Embryo transfer and the vitrification of embryos could be used for the conservation and recovery of endangered donkey breeds. It is important to develop techniques that optimize recovery rates and the cryotolerance of donkey embryos. This study evaluates factors affecting the recovery rate, quality, and diameter of embryos obtained from donor jennies as a starting point for the use of vitrification and embryo transfer in the conservation of the Andalusian donkey. A total of 100 embryos were recovered out of 124 estrous cycles (80.6%). The donor jenny affected the rates of positive flushings (P...
Su Y, Zhu J, Salman S, Tang Y.The development of the induced pluripotent stem cells (iPSCs) technology has revolutionized the world on the establishment of pluripotent stem cells (PSCs) across a great variety of animal species. Generation of iPSCs from domesticated animals would provide unrestricted cell resources for the study of embryonic development and cell differentiation of these species, for screening and establishing desired traits for sustainable agricultural production, and as veterinary and preclinical therapeutic tools for animal and human diseases. Induced PSCs from domesticated animals thus harbor enormous sc...
Fenner K, Dashper K, Serpell J, McLean A, Wilkins C, Klinck M, Wilson B, McGreevy P.The Equine Behaviour Assessment and Research Questionnaire (E-BARQ) is a questionnaire instrument developed to obtain quantitative data on the domestic equine triad of training, management, and behaviour of horses. The E-BARQ was developed to identify how changes in training and management impact behaviour over time, to define normal behaviour in horses, and to discover how to improve rider safety and horse welfare, leading to ethical equitation. During the development of the E-BARQ, we also investigated how best to motivate stakeholders to engage with this citizen science project. The pilot v...
Vernemmen I, Vera L, Van Steenkiste G, van Loon G, Decloedt A.Echocardiographic reference intervals for Friesian horses are poorly described. Objective: To obtain reference intervals for echocardiographic measurements in Friesians and compare these with Warmbloods. Methods: One hundred healthy adult Friesians and 100 healthy adult Warmblood horses. Methods: Cross-sectional study. Two-dimensional and M-mode echocardiographic images were obtained. Echocardiographic measurements, including size, area, and volumetric measurements of left atrium, left and right ventricle, aorta, and pulmonary artery, were performed. Measurements were compared between the 2 br...
Jansson A, Lindgren G, Velie BD, Solé M.Infrared thermography (IRT) is a popular technology used for the detection of thermal changes given its non-invasive nature and lack of direct contact with the individual. Accordingly, the maximal eye temperature (MaxET) measured with IRT has been extensively applied in equine research. However, there is little information available about the potential limitations of the MaxET in field studies. Thus, the aims of this study were to 1) quantify the individual variation of MaxET in field conditions and the effects of individual, breed, body size (height at withers), eye side, sex and age, 2) dete...
Baillet H, Leroy D, Vérin E, Delpouve C, Boulanger J, Benguigui N, Komar J, Thouvarecq R.The present research aims at quantifying the impact of practicing a new coordination pattern with an online visual feedback on the postural coordination performed on a mechanical horse. Forty-four voluntary participants were recruited in this study. They were randomly assigned to four practice groups based on i) with or without feedback (i.e., group 1, control, did not receive the feedback; group 2, 3 and 4 received an online feedback during practice) and ii) the specific trunk/horse coordination to target during practice (group 1, target coordination = 180° (without feedback); group 2, targe...
Labruna MB, Faccini JLH.Dermacentor nitens is a one-host tick that uses domestic equids as main hosts. Herein, the nonparasitic phase of D. nitens was evaluated under field conditions in Pirassununga, São Paulo state, Southeastern Brazil. By exposing engorged females of D. nitens to field conditions (grass plots) for 24 consecutive months, this tick species was able to complete its nonparasitic phase by producing host-seeking larvae in the pasture throughout the year. Preoviposition and egg incubation periods were longer during autumn and winter months than during the other months. The number of larvae generated by ...
Prieto A, Martins Almeida Ayupe K, Nemetala Gomes L, Saúde AC, Gutierres Filho P.Evaluate the effects of Equine-Assisted Therapy (EAT) on the functioning of individuals with different health conditions through a systematic review and meta-analysis of randomized clinical trials (RCT). Unassigned: This review was registered in the International Prospective Register of Systematic Reviews (CRD42018100035) and followed the Preferred Reporting Items for Systematic Review and Meta-analyses recommendations. RCT were searched in eight different databases, using relevant keywords until July 7, 2020. Two independent reviewers performed selection of articles and data extraction that w...
Salau J, Hildebrandt F, Czycholl I, Krieter J.Sensors delivering information on the position of farm animals have been widely used in precision livestock farming. Global Positioning System (GPS) sensors are already known from applications in military, private and commercial environments, and their application in animal science is increasing. However, as trade-offs between sensor cost, battery life and sensor weight have to be made, GPS based studies scheduling long data collection periods and including a high number of animals, have to deal with problems like high hardware costs and data disruption during recharging of sensors. Furthermor...
Serra Bragança FM, Broomé S, Rhodin M, Björnsdóttir S, Gunnarsson V, Voskamp JP, Persson-Sjodin E, Back W, Lindgren G, Novoa-Bravo M, Gmel AI....For centuries humans have been fascinated by the natural beauty of horses in motion and their different gaits. Gait classification (GC) is commonly performed through visual assessment and reliable, automated methods for real-time objective GC in horses are warranted. In this study, we used a full body network of wireless, high sampling-rate sensors combined with machine learning to fully automatically classify gait. Using data from 120 horses of four different domestic breeds, equipped with seven motion sensors, we included 7576 strides from eight different gaits. GC was trained using several ...
Pinto P, Hirata S.In some feral horse populations, adult females are either associated with a single male or multiple males (stallions). However, little is known on why such groups with more than one male exist, considering that stallions fight to monopolize females. Body size is often an important determinant of male fighting ability and/or dominance rank and, consequently, reproductive success. Stallions may, therefore, vary in "quality" which could be a differentiating factor between single-stallion and multi-stallion males. We observed feral Garrano horses in Northern Portugal and examined the relationship ...
Moeller BC, Yang Z.Controlling the abuse of prohibited substances such as anabolic steroids, selective androgen receptor modulators, β-adrenoceptor agonists, and blood doping agents is of great interest to racing authorities. The use of dried blood spots (DBS) as an alternative sampling approach may be a feasible approach for controlling the use of these agents. To assess the feasibility of using DBS in equine blood, an 11-min liquid chromatography-mass spectrometry method was developed on a triple quadrupole mass spectrometer following extraction from Whatman 903 DBS cards. A total of 50 compounds across multi...
Gomez A, Sharma AK, Grev A, Sheaffer C, Martinson K.Although contributions of the equine gut microbiome to forage utilization are well recognized, the impact of alfalfa (Medicago sativa L.) lignification on the equine gut microbiome remains unknown; thus, we characterized microbial communities in the equine gut when feeding reduced lignin (RL) and conventional (CON) alfalfa hays to adult stock-type horses. Dietary treatments were fed to six horses in a crossover study. Experimental periods consisted of a 9-day dietary adaptation phase followed by a 5-day total fecal collection phase, during which horses were housed in individual box stalls and ...
Rakowska A, Cywinska A, Witkowski L.The aim of this review was to summarize studies on equine rhodococcosis over the last decade. For many years has remained one of the major health challenges in the equine breeding industry worldwide. Recently, many novel approaches and ideas have been described and some of them were initially implemented into the clinical practice. This study reviews a variety of new information about neonatal susceptibility, clinical appearance, considered and applied diagnostic procedures and treatment alternatives, factors limiting accurate prognosis, ideas regarding environmental management and prophylax...
Kammerer R, Ballesteros A, Bonsor D, Warren J, Williams JM, Moore T, Dveksler G.In early equine pregnancy, a highly invasive trophoblast cell subpopulation, the chorionic girdle cells, invade the endometrium and form endometrial cups (EC). These cells express classical MHC molecules, thereby stimulating a humoral and cellular immune response, resulting in a massive accumulation of maternal CD4+ and CD8+ T cells around the EC. Nevertheless, no immediate destruction of endometrial cups by maternal lymphoid cells occurs, presumably due to immune tolerance. Although the environment of EC is rich in TGFB and in FOXP3+, CD4+ T cells, the mechanisms leading to tolerance have not...
MacKechnie-Guire R, Fisher M, Pfau T.Using a half pad beneath a saddle can be beneficial for improving saddle fit. However, there is a paucity of evidence on half pad use when used beneath a correctly fitted saddle. The aim was to quantify the effect that three different half pads have on pressure distribution beneath a saddle fitted following industry guidelines. Twelve nonlame horses were ridden by experienced riders in sitting trot and canter on each rein (three repeats). Saddle fit, with a high-withered cotton saddle cloth (control) compared with three half pads (viscoelastic gel, wool, and medical-grade, closed-cell foam), w...
Regan CE, Medill SA, Poissant J, McLoughlin PD.The adult sex ratio (ASR) is important within ecology due to its predicted effects on behaviour, demography and evolution, but research examining the causes and consequences of ASR bias have lagged behind the studies of sex ratios at earlier life stages. Although ungulate ASR is relatively well-studied, exceptions to the usual female-biased ASR challenge our understanding of the underlying drivers of biased ASR and provide an opportunity to better understand its consequences. Some feral ungulate populations, including multiple horse populations, exhibit unusually male-biased ASR. For example, ...
White JL, Salinas EY, Link JM, Hu JC, Athanasiou KA.A significant portion of equine lameness is localized to the stifle joint. Effective cartilage repair strategies are largely lacking, however, recent advances in surgical techniques, biomaterials, and cellular therapeutics have broadened the clinical strategies of cartilage repair. To date, no studies have been performed directly comparing neonatal and adult articular cartilage from the stifle across multiple sites. An understanding of the differences in properties between the therapeutic target cartilage (i.e., adult cartilage) as well as potential donor cartilage (i.e., neonatal cartilage) c...
Consuegra C, Crespo F, Dorado J, Diaz-Jimenez M, Pereira B, Sánchez-Calabuig MJ, Beltrán-Breña P, Pérez-Cerezales S, Rizos D, Hidalgo M.The aim of this study was to evaluate the fertilizing capacity of frozen or vitrified stallion sperm after assessing different warming procedures. In Experiment 1, different warming procedures were compared after sperm vitrification: immersion in extender at 43 °C (C), or in a water bath at 37 °C/30 s (W37), 43 °C/10 s (W43) or 60 °C/5 s (W60). With the W60 treatment, there were greater values (P < 0.05) for VCL (83.93 ± 3.6 μm/s) and ALH (3.00 ± 0.2 μm) than freezing and with the C group, and greater values (P < 0.001) for PM (35.33 ± 2.5 %) than with the W43 treatment. In Experiment...
True CK, Dotzel AR.Oral endoscopy is a valuable addition to the equine dental examination process. It enables veterinarians to visualize subtle oral disorders and is a useful client education tool. There are several commercially available oral endoscopic systems on the market. Practitioners can also assemble their own systems. An oral endoscope is used to perform a thorough and systematic oral examination and to visualize normal oral structures as well as oral disorders. It is also used to guide instrument placement during oral surgeries and other dental procedures.
Epperly E, Whitty JA.Computed tomography (CT) has revolutionized the veterinarian's ability to image the equine skull and led to improved diagnostic accuracy and clarity for surgical planning. The increased cost for this evaluation is offset by more accurate diagnosis and targeted therapy. As novel technology is developed that allows for increased availability of equine head, the price will continue to decrease and more examinations will be performed. New skills are needed for the veterinarian to accurately interpret this modality. This article reviews the normal CT appearance of the equine skull and presents exam...
Henry T, Bishop I.Adjunct extraction techniques are used when the shape or integrity of the tooth, the shape or size of the patient's oral cavity, the location of the tooth, or the location of the proximal teeth prevents or complicates standard oral extraction. Techniques described and discussed include partial coronectomy, tooth sectioning, minimally invasive buccotomy, transbuccal screw extraction, and commissurotomy.
Herrera M, León G, Segura A, Meneses F, Lomonte B, Chippaux JP, Gutiérrez JM.Caprylic acid purification of IgG, currently used in the manufacture of horse-derived antivenoms, was successfully adapted for the preparation of sheep and camel IgG. Sheep IgG had a molecular mass of approximately 150 kDa, whereas camel IgG presented two bands of molecular masses of approximately 160 and 100 kDa, the latter corresponding to heavy-chain IgG, which is devoid of light chains. Horse, sheep and camel IgGs were compared by several parameters aiming at predicting their potential for induction of early and late adverse reactions. Horse and sheep IgGs showed a higher anticomplementary...
Holl HM, Pflug KM, Yates KM, Hoefs-Martin K, Shepard C, Cook DG, Lafayette C, Brooks SA.Variations in the SLC45A2 gene are responsible for the dilution phenotypes cream and pearl in domestic horses. Cream dilution is inherited in an incomplete dominant manner, diluting only red in the heterozygous state but both red and black pigments when two alleles are present. The pearl dilution is recessive and dilutes only the red and black pigment in the homozygous state or when paired with a cream allele. Horses that inherit one copy of pearl (C ) and one copy of the dominant cream allele (C ) display a dilution phenotype similar to that of homozygous cream, suggesting that pearl is the ...
Ronéus BO, Hakkarainen RV, Lindholm CA, Työppönen JT.Vitamin E requirements of adult Standardbred horses were evaluated by tissue depletion and repletion. All the horses used in the study were given the same basal feed low in vitamin E during the eight months of the experiment. After an initial depletion period of two-and-a-half months the horses were divided into groups according to the amounts of DL alpha-tocopheryl acetate given (0 mg, control; 200, 600, 1800 and 5400 mg, respectively) as a daily oral supplement. The supplement study was followed by a second depletion period. Total vitamin E content and individual natural tocopherol isomers a...
Egito AS, Miclo L, López C, Adam A, Girardet JM, Gaillard JL.The equine alpha(s1)- and beta-caseins (CN) were purified by chromatography on DEAE-cellulose and by reversed-phase HPLC. The alpha(s1)-, beta-, and kappa-CN were characterized either by monodimensional urea-PAGE or sodium dodecylsulfate (SDS)-PAGE or by bidimensional electrophoresis. Kappa-casein was characterized after electrophoresis by glycoprotein-specific staining. To identify alpha(s1)-CN without ambiguity, internal sequences were determined after trypsin or chymosin digestion of purified alpha(s1)-CN. These sequences, that could be estimated to correspond to 62% of the full protein, pr...
Tirosh-Levy S, Gottlieb Y, Arieli O, Mazuz ML, King R, Horowitz I, Steinman A.Equine piroplasmosis (EP) is an important tick-borne disease of equids, caused by Theileria equi and Babesia caballi. It is endemic in most parts of the world, including Israel, and has clinical and economic consequences. This study was set to evaluate the presence of EP parasites in domestic donkeys and in wild equids in Israel and the Palestinian Authority (PA). To assess subclinical EP infection in 98 domestic donkeys (Equus africanus asinus), 9 Asiatic wild donkeys (Equus hemionus), 8 zebras (Equus quagga), 7 African wild donkeys (Equus africanus) and 5 mules, were tested using PCR and qPC...
Maigrot AL, Hillmann E, Briefer EF.Discrimination and perception of emotion expression regulate interactions between conspecifics and can lead to emotional contagion (state matching between producer and receiver) or to more complex forms of empathy (e.g., sympathetic concern). Empathy processes are enhanced by familiarity and physical similarity between partners. Since heterospecifics can also be familiar with each other to some extent, discrimination/perception of emotions and, as a result, emotional contagion could also occur between species. Here, we investigated if four species belonging to two ungulate Families, Equidae (d...
Hisey E, Ross PJ, Meyers SA.OCT4 is a core transcription factor involved in pluripotency maintenance in the early mammalian embryo. The POU5F1 gene that encodes the OCT4 protein is highly conserved across species, suggesting conserved function. However, studies in several species including mice, cattle, and pigs, suggest that there are differences in where and when OCT4 is expressed. Specifically, in the horse, several studies have shown that exposure to the uterine environment may be necessary to induce OCT4 expression restriction to the inner cell mass (ICM) of the developing embryo, suggesting that there may be equine...
Fortin M, Valenchon M, Lévy F, Calandreau L, Arnould C, Lansade L.Emotions are recognized as strong modulators of cognitive capacities. However, studies have mainly focused on the effect of negative emotions, with few investigating positive emotions. Recent studies suggest that traits of personality can modulate the effects of emotion on cognitive performance. This study aimed to assess whether emotional states differing according to their valence influenced the ability to achieve instrumental conditioning and learning flexibility and to determine the influence of personality. After being tested for their personality, 55 mares underwent acquisition and extin...
Flethøj M, Schwarzwald CC, Haugaard MM, Carstensen H, Kanters JK, Olsen LH, Buhl R.Prolonged exercise in human athletes is associated with transient impairment of left ventricular (LV) function, known as cardiac fatigue. Cardiac effects of prolonged exercise in horses remain unknown. Objective: To investigate the effects of prolonged exercise on LV systolic and diastolic function in horses. Methods: Twenty-six horses competing in 120-160 km endurance rides. Methods: Cross-sectional field study. Echocardiography was performed before and after rides, and the following morning, and included two-dimensional echocardiography, anatomical M-mode, pulsed-wave tissue Doppler imaging...
Zenker W, Snobl D, Boetschi R.The dependence of the inner organisation and innervation of a skeletal muscle on its size was studied at the level of single muscle fiber architecture and motor endplate topography in muscles of different size, all of them lacking a tendinous scaffolding. The muscles evaluated in this study were: Mm. sternomastoideus, gracilis and latissimus dorsi of the rat and the M. sternocephalicus of the horse. In these muscles a subdivision into two or more 'innervation-compartments' becomes obvious in fascicles reaching a certain length. This provides the possibility of an almost synchronous activation ...
Clauss M, Schiele K, Ortmann S, Fritz J, Codron D, Hummel J, Kienzle E.Equid digestion is often conceptualized as a high-throughput/low-efficiency system, in particular compared with ruminants. It is commonly assumed that ruminants have an advantage when resources are limited; the effect of low food intake on digestive physiology of horses has, however, not been explored to our knowledge. We used four adult ponies [initial body mass (BM) 288 ± 65 kg] in two subsequent trials with grass hay-only diets [in dry matter (DM): hay1, mid-early cut, crude protein (CP) 10.5%, neutral detergent fibre (NDF) 67.6%; hay2, late cut, CP 5.8%, NDF 69.5%], each fed subsequent...
Bundgaard L, Stensballe A, Elbæk KJ, Berg LC.Stem cells have great potential for tissue regeneration, but before stem cell populations can be used in the clinic, it is crucial that the stem cells have been definitely characterized by a set of specific markers. Although there have been attempts to identify a set of immunophenotypic markers to characterize equine mesenchymal stromal cells (MSCs), immunophenotyping of equine MSCs is still challenging due to the limited availability of suitable antibodies of high quality and consistent performance across different laboratories. The aim of this study was to evaluate a strategy for mapping the...
Tozaki T, Ohnuma A, Takasu M, Nakamura K, Kikuchi M, Ishige T, Kakoi H, Hirora KI, Tamura N, Kusano K, Nagata SI.Gene doping has raised concerns in human and equestrian sports and the horseracing industry. There are two possible types of gene doping in the sports and racing industry: (1) administration of a gene-doping substance to postnatal animals and (2) generation of genetically engineered animals by modifying eggs. In this study, we aimed to identify genetically engineered animals by whole-genome resequencing (WGR) for gene-doping control. Transgenic cell lines, in which the erythropoietin gene (EPO) cDNA form was inserted into the genome of horse fibroblasts, were constructed as a model of genetica...
Richard F, Messaoudi C, Lombard M, Dutrillaux B.Using human chromosome painting probes, we looked for homologies between human and mountain zebra (Equus zebra hartmannae, Equidae, Perissodactyla) karyotypes. Except for two very short segments, all euchromatic regions were found to have a human homologous chromosome segment. Conserved syntenies previously described in various mammalian orders were detected. Each synteny corresponded to a chromosomal region homologous to two parts of human chromosomes: HSA3 and HSA21, HSA7 and HSA16, HSA12 and HSA22, and HSA16 and HSA19. Chromosomal segments homologous to a part of HSA11 and HSA19p are found ...
Goodrich EL, Mittel LD, Glaser A, Ness SL, Radcliffe RM, Divers TJ.This case report describes an outbreak and novel findings associated with a beta coronavirus (BCoV) infection that occurred on an American Miniature Horse (AMH) breeding farm in upstate New York, in January and February of 2013. Twenty-nine AMH and one donkey were present on the farm when the outbreak occurred. One 10-year-old Quarter Horse mare, stabled at a separate location and owned by an employee of the farm, also tested positive. A polymerase chain reaction (PCR) assay for the detection of BCoV was performed at the Animal Health Diagnostic Center (AHDC) at Cornell on all faecal samples. ...
Godard S, Schibler L, Oustry A, Cribiu EP, Guérin G.A horse BAC library was constructed with about 40,000 clones and mean insert size of 110 kb representing a 1.5 genome equivalent coverage and a probability of finding a single sequence of 0.75. It was characterized by PCR screening of about 130 sequences of horse microsatellites and exonic gene sequences retrieved from databases. BACs containing 8 microsatellites and 12 genes were subsequently localized by fluorescent in situ hybridization (FISH) on chromosomes. Two linkage groups were newly assigned to chromosomes: LG2 to ECA3 and LG5 to ECA24, and five linkage groups were also oriented--LG3,...
Rogers CW, Firth EC.To identify the magnitude and sources of error involved in simply quantifying the workload of a group of Thoroughbred racehorses in training. Methods: Data were obtained from seven 2-year-old fillies trained for 13 weeks on sand and grass tracks at various cantering velocities, and at the gallop in the last 4 weeks. Four fillies completed training and comprised a 'high exercise intensity' subgroup; three fillies did not complete all gallops due to lameness or injury and comprised a 'medium exercise intensity' subgroup. The trainer recorded the distance, time, track used, and subjective gait (S...
Messick JB, Rikihisa Y.The effects of equine antiserum, immunoglobulin G (IgG) specific for Ehrlichia risticii, and its Fab fragment on E. risticii binding to, internalization into, and proliferation in P388D1 cells were studied by immunofluorescence flow cytometry. Anti-E. risticii equine serum or IgG inhibited E. risticii at a stage beyond binding and internalization. In contrast, monovalent anti-E. risticii equine Fab fragments inhibited E. risticii binding and internalization into P388D1 cells. In the presence of control equine serum, IgG, or its Fab fragment, E. risticii cells were bound, were internalized and ...
Carnevale EM.Assisted reproductive techniques that are based on oocyte manipulations have gained acceptance in the equine industry. Methods to collect and handle immature or maturing oocytes have been developed, and systems to ship oocytes now allow for collection in one location and intracytoplasmic sperm injection (ICSI) in another. Subsequently, ICSI-produced embryos can be transferred onsite, shipped to another location, or cryopreserved. Methods for the collection, identification, culture, maturation, and shipment of equine oocytes are reviewed, with an emphasis on procedures from laboratories providi...
Gigante A, Bevilacqua C, Cappella M, Manzotti S, Greco F.Articular cartilage defects do not heal. Biodegradable scaffolds have been studied for cartilage engineering in order to implant autologous chondrocytes and help cartilage repair. We tested some new collagen matrices differing in collagen type, origin, structure and methods of extraction and purification, and compared the behavior of human chondrocytes cultured on them. Human chondrocytes were grown for three weeks on four different equine type I collagen matrices, one type I, III porcine collagen matrix and one porcine type II collagen matrix. After 21 days, samples were subjected to histoche...
van der Kuyl AC.Information on endogenous retroviruses fixed in the horse (Equus caballus) genome is scarce. The recent availability of a draft sequence of the horse genome enables the detection of such integrated viruses by similarity search. Using translated nucleotide fragments from gamma-, beta-, and delta-retroviral genera for initial searches, a full-length beta-retrovirus genome was retrieved from a horse chromosome 5 contig. The provirus, tentatively named EqERV-beta1 (for the first equine endogenous beta-retrovirus), was 10434 nucleotide (nt) in length with the usual retroviral genome structure of 5'...
Jansson A, Gunnarsson VÞ, Ringmark S, Ragnarsson S, Söderroos D, Ásgeirsson E, Jóhannsdóttir TR, Liedberg C, Stefánsdóttir GJ.This study examined the effect of altered body weight (BW) and body fat content on exercise performance and recovery. Nine horses were divided into two groups, and changes in BW and fat content were induced by feeding a high (HA) or restricted (RA) energy allowance for 36 days in a cross-over design. In the last week of each treatment, BW and body condition score (BCS) were recorded, body fat percentage was estimated using ultrasound, and a standardized incremental treadmill exercise test (SET) and competition-like field test were performed (scored by judges blinded to treatments). Blood samp...
Raudsepp T, Lear TL, Chowdhary BP.The X chromosomes of the extant equids, in general, share morphology and banding pattern similarities. However, the donkey X is, in part, an exception because of significantly different centromeric index and variant banding patterns in the pericentromeric region. To verify the underlying molecular basis of this difference, twelve equine BAC clones were FISH mapped to donkey (EAS) and Hartmann's mountain zebra (EZH) metaphase spreads. Loci from the terminal region of Xp and distal to terminal regions of the Xq showed the same order and relative position in all three species, implying cross-spec...
Broeckx SY, Maes S, Martinello T, Aerts D, Chiers K, Mariën T, Patruno M, Franco-Obregón A, Spaas JH.Besides the presence of somatic stem cells in hair follicles and dermis, the epidermis also contains a subpopulation of stem cells, reflecting its high regenerative capacity. However, only limited information concerning epidermis-derived epithelial-like stem/progenitor cells (EpSCs) is available to date. Nonetheless, this stem cell type could prove itself useful in skin reconstitution after injury. After harvesting from equine epidermis, the purified cells were characterized as EpSCs by means of positive expression for CD29, CD44, CD49f, CD90, Casein Kinase 2β, p63, and Ki67, low expression f...
Hovey MR, Davis A, Chen S, Godwin P, Porr CAS.Stress can impact the health and well-being of animals negatively. Behavioral and physiological changes, particularly serum cortisol, offer objective and easy-to-use methods of evaluating stress in horses. However, limited studies support a positive relationship between changes in stress-related serum cortisol concentrations and stress-related behaviors in horses. This study assessed differences in stress-related behaviors and serum cortisol concentrations in horses used in a therapeutic riding program (TRH) or university riding program (UNI). Riders were grouped by disability type (TRH) or by...
Handing KB, Shabalin IG, Szlachta K, Majorek KA, Minor W.Serum albumin (SA) is the main transporter of drugs in mammalian blood plasma. Here, we report the first crystal structure of equine serum albumin (ESA) in complex with antihistamine drug cetirizine at a resolution of 2.1Å. Cetirizine is bound in two sites--a novel drug binding site (CBS1) and the fatty acid binding site 6 (CBS2). Both sites differ from those that have been proposed in multiple reports based on equilibrium dialysis and fluorescence studies for mammalian albumins as cetirizine binding sites. We show that the residues forming the binding pockets in ESA are highly conserved in h...
Pouran B, Arbabi V, Zadpoor AA, Weinans H.The metabolic function of cartilage primarily depends on transport of solutes through diffusion mechanism. In the current study, we use contrast enhanced micro-computed tomography to determine equilibrium concentration of solutes through different cartilage zones and solute flux in the cartilage, using osteochondral plugs from equine femoral condyles. Diffusion experiments were performed with two solutes of different charge and approximately equal molecular weight, namely iodixanol (neutral) and ioxaglate (charge=-1) in order to isolate the effects of solute's charge on diffusion. Furthermore,...
Shibeshi W, Abraham G, Kneuer C, Ellenberger C, Seeger J, Schoon HA, Ungemach FR.Culture of airway epithelial cells is a useful model to investigate physiology of airway epithelia and airway disease mechanisms. In vitro models of airway epithelial cells are established for various species. However, earlier published method for isolation and culture of equine tracheal epithelial cells requires significant improvements. In this report, the development of a procedure for efficient isolation, characterization, culture, and passage of primary equine tracheal epithelial cells are described. Epithelial cells were isolated from adult equine trachea by exposing and stripping the mu...
Marchand WR.Equine-assisted services (EASs) are being increasingly used as complementary interventions for military veterans who have experienced trauma. However, there is limited evidence of benefit for this population and almost no literature describing the desired potential outcomes and possible mechanisms of action. The aim of this article is to address these gaps by reviewing the extant literature of animal-assisted interventions in general, and equine-assisted services in particular, with the goal of providing guidance for future investigations in the field. Currently, the field is in the early stag...
Mitchell KJ.Analyzing electrocardiographic (ECG) recordings, making a diagnosis and assessment of any arrhythmias present, is an important part of the workup of many equine cases. Accurate analysis requires a good-quality recording, free of as many artifacts as possible, with clear P-QRS-T complex morphology. For sustained arrhythmias, short-term recordings are sufficient to make the appropriate diagnosis before instigating treatment. Longer-term recordings are essential for arrhythmias that are paroxysmal, intermittent, or occurring infrequently, while exercising ECGs are required for arrhythmias associa...
