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Topic:Horses
"Horses" is a broad topic that encompasses various aspects of equine biology, behavior, and management. This category includes studies on the anatomy, physiology, and genetics of horses, as well as their behavior, nutrition, and care. Research in this area may also cover the historical and cultural significance of horses, their roles in agriculture, sport, and therapy, and the challenges associated with their conservation and welfare. The page aggregates peer-reviewed research articles and scholarly studies that explore the multifaceted relationships between humans and horses, examining both scientific and socio-economic perspectives.
Combined hepatocellular carcinoma and cholangiocarcinoma in a mare. A hepatic malignant tumour composed of both hepatocellular and cholangiocellular elements was studied histologically, immunohistochemically and electron microscopically in an 18-year-old Thoroughbred mare. Bile canaliculi and alpha-fetoprotein were useful in identifying the hepatocellular element, and mucin and keratin were good markers of biliary differentiation. The simultaneous presence of bile canaliculi and mucin-producing cells in most of the neoplastic lesions suggested that this tumour arose from a stem cell with capacity to differentiate into hepatocytes and biliary epithelium.
Serodiagnosis of Babesia equi infection–a comparison of Dot-ELISA, complement fixation test and capillary tube agglutination test. The present study aimed to develop Dot-ELISA, complement fixation test (CFT) and capillary tube agglutination test (CAT) for serodiagnosis of Babesia equi infection and to compare their sensitivity with each other. For this study, sequential serum samples were collected from four donkeys experimentally infected with B. equi up to 90 days post infection (P.I.). B. equi antigen was prepared from the blood of a donkey showing more than 80% parasitaemia. Dot-ELISA, CF and CA tests were standardized as per the standard method. While performing CFT, it was observed that CFT standardized for the donk...
Magnetic resonance imaging of the equine metacarpophalangeal joint: three-dimensional reconstruction and anatomic analysis. Magnetic resonance imaging was used to examine the equine metacarpophalangeal joint. Thirty-two saggital images generated by partial volume imaging were transferred to a computer for three-dimensional reconstruction and analysis. All the tissues constituting the metacarpophalangeal joint were readily identified. The most significant increase finding regarded the soft tissues on the palmar aspect of the metacarpophalangeal joint and their interactions with the proximal sesamoid bones. The equine metacarpophalangeal joint has not previously been evaluated using 3-dimensional imaging software.
A light microscopic and ultrastructural examination of calcified dental tissues on horses: 4. Cement and the amelocemental junction. Ultrastructural examinations showed the diameter of cement lacunae to be greater in infundibular cement than in peripheral cement of upper cheek teeth, which in turn was greater than in the peripheral cement of the lower cheek teeth. However, numbers of lacunae/unit area remained similar in these 3 dentinal region. Two types of cemental hypoplasia were found in equine cheek teeth. The first type was termed central infundibular cemental hypoplasia and was confined to the central region of infundibular cement. The cement adjacent to these frequently large defects was very porous and contained la...
Functional characterization of equine neutrophils in response to calcium ionophore A23187 and phorbol myristate acetate ex vivo. Equine neutrophils (PMN) play a critical role in inflammatory processes in horses. The objective of this study was to characterize equine PMN function ex vivo following stimulation with calcium ionophore A23187 (A23187) and phorbol myristate acetate (PMA). These stimulants trigger different branches of the PMN activation process that occurs in vivo. Equine PMN were isolated from the whole blood of six clinically normal geldings using a one-step discontinuous Percoll gradient technique. Neutrophil aggregation, degranulation, and superoxide anion production were evaluated in assay systems which ...
A light microscopic and ultrastructural examination of calcified dental tissues of horses: 1. The occlusal surface and enamel thickness. Gross and microscopic examinations were undertaken on 46 cheek (molar and premolar) and 4 incisor equine teeth that were fractured, or sectioned either with a lathe or diamond saw. Specimens were examined without treatment, after decalcification or acid etching, utilising light, and scanning and transmission electron microscopy. In some horses, the occlusal surface of the teeth were covered with an organic pellicle. The occlusal surface of the underlying equine enamel contained different wear patterns, including polished areas, local fractures, wedge-shaped pits, striations and depressions. Oc...
Oncotic, hemodilutional, and hemostatic effects of isotonic saline and hydroxyethyl starch solutions in clinically normal ponies. To evaluate the oncotic, hemodilutional, and hemostatic effects of IV infusions of a large volume of isotonic saline solution and 2 doses of 6% hydroxyethyl starch (HES) in clinically normal ponies. Methods: 12 adult ponies. Methods: Ponies were assigned to 3 treatment groups and received the following IV infusions: 80 ml of 0.9% sodium chloride/kg; 10 ml of 6% HES (in 0.9% sodium chloride)/kg; or 20 ml of 6% HES (in 0.9% sodium chloride)/kg. Blood samples were collected for determination of colloid oncotic pressure (COP), PCV, plasma total protein concentration, platelet count, von Willebrand...
A light microscopic and ultrastructural examination of calcified dental tissues of horses: 3. Dentine. Ultrastructural examinations of equine dentine found that dentinal tubules extended from the amelodentinal junction towards the pulp forming primary curvatures. The number of dentinal tubules/unit area and their diameters increased significantly from the amelodentinal junction towards the pulp cavities, particularly in regular secondary dentine, but irregular secondary (tertiary) dentine contained no dentinal tubules. Dentinal tubules contained odontoblast processes that appeared to extend as far as the amelodentinal junction, but due to iatrogenic loss during specimen preparation, odontoblast...
Detection of African horse sickness virus in the blood of experimentally infected horses: comparison of virus isolation and a PCR assay. A reverse transcription-polymerase chain reaction (RT-PCR) assay followed by dot-blot hybridisation was used to detect African horse sickness virus (AHSV); the primers employed amplified the S7 gene that encodes the VP7 protein. The RT-PCR assay was compared with virus isolation for detecting AHSV in blood samples form horses experimentally infected with AHSV-4 and AHSV-9. The influence of sample storage and transportation and the effects of two anticoagulants (EDTA and heparin) were also studied. RT-PCR results were obtained within 48 hours as opposed to a minimum of 15 days for virus isolati...
Impact during equine locomotion: techniques for measurement and analysis. Impact is implicated in the development of several types of musculoskeletal injury in the horse. Characterisation of impact experienced during strenuous exercise is an important first step towards understanding the mechanism for injury. Measurement and analysis of large, short duration impacts is difficult. The measurement system must be able to record transient peaks and high frequencies accurately. The analysis technique must be able to characterise the impact signal in time and frequency. This paper presents a measurement system and analysis technique for the characterisation of large impac...
The relationship between the concentration of ionised calcium and parathyroid hormone-related protein (PTHrP[1-34]) in the milk of mares. Once lactation is established in mares, there is little change in the ionised calcium concentration in their milk. In contrast, the concentration of PTHrP(1-34) in the milk increases to a maximum level by the end of the second week of lactation, near which it remains for the rest of the lactation. As found in other species, the concentration of PTHrP(1-34) in mare's milk is considerably higher than that in plasma, sampled at the same time. No significant correlation could be demonstrated between the concentrations of PTHrP(1-34) and ionised calcium in the milk except during the last 10 weeks o...
Detection of latency-associated transcripts of equid herpesvirus 1 in equine leukocytes but not in trigeminal ganglia. Results from Southern hybridization and PCR amplification experiments using a randomly synthesized reverse transcription-PCR product showed that peripheral blood leukocytes from horses showing no clinical signs of disease expressed a putative latency-associated transcript antisense to and overlapping the 3' end of the equid herpesvirus 1 (EHV-1) immediate-early gene (gene 64). A PCR product derived from this transcript has > or =96% identity with the published EHV-1 sequence. In situ hybridization studies of equine bronchial lymph nodes corroborated these findings and are consistent with re...
The effects of ischemia and reperfusion on mucosal respiratory function, adenosine triphosphate, electrolyte, and water content in the ascending colon of ponies. The purpose of this study was to examine the effects of ischemia and reperfusion on the biochemical integrity of equine colonic mucosa to assess the relative roles of ischemic- and reperfusion-induced damage. Methods: Two hours of no-flow ischemia experimentally induced by 720 degrees counterclockwise ascending colon volvulus followed by 2 hours reperfusion after derotation. Methods: Ten ponies. Methods: Ascending colon biopsies were obtained every hour for measurement of mucosal adenosine triphosphate (ATP), water, sodium, and potassium content. Additional samples were homogenized for assay o...
Components of the total kinetic moment in jumping horses. Thirty horses were filmed with a panning camera operating at 50 frames/s as they jumped over a 1.20 x 1.20 m fence. The markers of 9 joints on the horse and 7 joints on the rider were tracked in 2D with the TrackEye system. The centre of gravity and moment of inertia of each segment were calculated using a geometric algorithm and a cylindric model, respectively. The kinetic moment of each part of the horse was calculated after filtering, and resampling of data. This method showed the relative contribution of each body segment to the body overall rotation during the take-off, jump and landing p...
Comparison of three methods of ulnar fixation in horses. This study compares the mechanical properties of three methods of equine ulnar fixation: dynamic compression plating, pins and wires tension band, and a prototype grip system. Methods: The mechanical properties of dynamic compression plating, pins and wires tension band, and a prototype grip system repair of equine ulnar fractures were evaluated in a cadaveric osteotomy model. Methods: Fifteen pairs of the radius and ulna from equine cadavers. Methods: The three repair techniques were evaluated to mimic the pull of the triceps brachii muscle in single cycle to failure and in cyclic fatigue loa...
Detection and distribution of equine herpesvirus 2 DNA in the central and peripheral nervous systems of ponies. The distribution of equine herpesvirus 2 (EHV-2) DNA within neurological and lymphoid tissues from 12 EHV-2 seropositive Welsh mountain ponies was determined by PCR. The lymphoid sites sampled in this study were almost universally PCR positive, thus confirming the existing virus co-cultivation data which suggest that the lymph nodes draining the respiratory tract are the main reservoirs of EHV-2 DNA. In addition, EHV-2 DNA was also detected, albeit with lower frequency, within both the peripheral and central nervous systems (PNS and CNS) of these animals. Of the CNS sites sampled 11% were PCR-...
Equine herpesvirus 4 DNA in trigeminal ganglia of naturally infected horses detected by direct in situ PCR. Neuronal and lymphoid tissues of 15 randomly selected horses were analysed post mortem by liquid nested-PCR to study the tropism of equine herpesvirus 4 (EHV-4). In four animals the trigeminal ganglia and in one case the lung were positive. Using a direct in situ PCR the EHV-4 genome was localized in the nuclei of neurons and in the bronchiolar as well as alveolar epithelium of the lung. In none of these tissues could infectious virus or viral antigens be detected. Applying the more sensitive liquid RT-PCR, however, an acute infection was demonstrated in one of the trigeminal ganglia by amplif...
Pulsed wave-Doppler ultrasonographic evaluation of the common carotid artery in the resting horse: physiologic data. A pulsed wave-Doppler ultrasonographic evaluation of common carotid arterial blood flow was carried out on 63 healthy Italian Saddlebred horses. Vessel diameter and tracing morphology were evaluated and blood flow parameters (systolic, diastolic and mean velocity, acceleration and deceleration of the systolic wave, carotid pulse volume) were calculated and correlated with class variables (sex, age and body weight). On the basis of the presence of an incisure in the ascending branch of the systolic curve, subjects were divided in two groups: one with a bifid systolic curve and the other with a ...
Effect of antigen challenge on the activation of peripheral blood neutrophils from horses with chronic obstructive pulmonary disease. The effect of antigen challenge on the state of activation of peripheral blood neutrophils from horses with chronic obstructive pulmonary disease (COPD) has been determined by measuring neutrophil superoxide anion formation. Prior to a seven-hour antigen challenge superoxide anion production by neutrophils from asymptomatic horses with COPD and normal horses in response to platelet activating factor (PAF) (with and without cytochalasin B), serum treated zymosan (STZ) and phorbol myristate acetate (PMA) was similar. Agonist-induced superoxide production by neutrophils from symptomatic COPD and ...
Comparison of the temporal kinematics of the canter pirouette and collected canter. The objectives were to compare the temporal characteristics of canter pirouette strides with collected canter strides in elite dressage horses, and to determine whether the stride kinematics of the canter pirouettes fulfilled the requirements specified in the Federation Equestre Internationale Rules for Dressage Events. Eleven horses were videotaped (60 fields/s) during the individual medal competition at the 1992 Olympic Games. Temporal variables were extracted from the videotapes using standard methods. Two strides were analysed on each of the left and right leads and these were pooled to gi...
Kinematic analysis of the locomotion symmetry of sound horses at a slow trot. This study of the locomotion symmetry was undertaken to provide standard symmetry indices of a group of sound horses at the trot. Using a 3D data collection system, the kinematics of the limb joints of 13 clinically nonlame horses were recorded while trotting in the standard conditions of the clinical lameness examination. A kinematic symmetry indice based on an inter-correlation method was defined and applied to the vertical displacement-time and joint angle-time diagrams of the left and right joints of the horses. For each horse, the mean symmetry indice of each joint was calculated using va...
Kinematic comparison of the leading and trailing fore- and hindlimbs at the canter. The canter is a 3 beat asymmetrical gait with a difference in timing between left and right limbs. To evaluate intralimb asymmetry at the canter, a group of 24 Dutch Warmbloods was evaluated on a treadmill (7 m/s) using a modified CODA-3 optoelectronic gait analysis system. Thirteen horses cantered in the left lead ('leading limb' group) and 11 in the right lead ('trailing limb' group) during left forelimb recordings, while 11 horses were at the left and 13 were at the right lead during left hindlimb recordings. Kinematic differences between horses from the 'leading limb' and 'trailing limb' g...
Acute myelogenous leukaemia in a mare. A 5-year-old Thoroughbred mare presented with a 4 week history of weight loss, fever and leukopenia. Rectally, a large active foetus, thickened spleen and an abdominal mass were palpated. Leukopenia, mild anaemia, marked thrombocytopenia and hyperfibrinogenaemia were found. Cytology and cytochemical staining of a bone marrow aspirate supported a diagnosis of acute myelogenous leukaemia. The mare deteriorated despite medical therapy and was humanely euthanased.
Effect of added weight on landing kinematics in jumping horses. Six event horses jumped a 1.10 m high table fence 4 times under each of 2 conditions; the rider weight condition involved carrying the weight of the rider and saddle (61 kg), whereas the added weight condition included an additional 18 kg weight cloth. Sagittal view, 60 Hz video recordings were analysed using standard methods. Comparisons between the rider weight and added weight conditions using paired t tests (P<0.05) showed a number of significant differences. In the added weight condition the leading forelimb landed closer to the fence, and there were increases in the maximal extension ...
