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Topic:Machine Learning

Machine learning is a branch of artificial intelligence that involves the development of algorithms and statistical models enabling computers to perform tasks without explicit instructions. In the context of equine research, machine learning can be applied to analyze large datasets related to horse health, behavior, and performance. It can assist in identifying patterns and correlations that may not be immediately apparent through traditional analysis methods. Applications of machine learning in equine studies include predicting disease outbreaks, assessing gait abnormalities, and optimizing breeding strategies. This page compiles peer-reviewed research studies and scholarly articles that explore the applications, methodologies, and outcomes of using machine learning techniques in the study of equine science.
Development of an Explainable Machine Learning Computational Model for the Prediction of Severe Complications After Orchiectomy in Stallions.
Animals : an open access journal from MDPI    January 25, 2026   Volume 16, Issue 3 377 doi: 10.3390/ani16030377
Tyrnenopoulou P, Kalatzis D, Kiouvrekis Y, Flouraki E, Folias L, Loukopoulos E, Starras A, Chalvatzis P, Tsioli V, Mavrogianni VS, Fthenakis GC.The objective of the present study was to apply supervised Machine Learning to predict severe complications after equine orchiectomy. A dataset of 612 cases of orchiectomies in stallions was used for the development of a computational model, among which in 8.5% of cases severe complications (colic, continued stallion-like behaviour, evisceration, funiculitis, haemorrhage, and scrotal infection) were diagnosed post-orchiectomy. Three supervised Machine Learning tools were employed: Logistic Regression (12 different models evaluated), Random Forest (64 models), and Gradient Boosting (8 models). ...
Niacin Improves Cryopreserved Equine Sperm Quality and Gene Expression: An Artificial Intelligence Assisted Evaluation.
Reproduction in domestic animals = Zuchthygiene    January 10, 2026   Volume 61, Issue 1 e70173 doi: 10.1111/rda.70173
Alves NC, Freitas MM, Faria JRD, Horta CL, Martins-Filho OA, Araújo MSS, Costa GMJ, Costa EA, de Almeida FRL, Amaral PHR, Pérez JCG, Lana ÂQ....Niacin acts as an antioxidant that protects cells from oxidative damage. This study evaluated the effects of adding niacin to the equine semen freezing extender on sperm quality and gene expression after cryopreservation. Ejaculates from ten stallions were frozen using the INRA 96 extender (control) or extenders supplemented with 10- and 20-mM niacin. After thawing, sperm were analysed for motility, kinematics, viability, membrane integrity, mitochondrial potential, lipid peroxidation, nitrite, hydrogen peroxide, malondialdehyde and reactive oxygen species (ROS) concentrations, DNA integrity, ...
Evaluating the Accuracy of a Vision-Based Algorithm for Groundline Estimation in Trotting Horses Using Multiple Camera Angles.
Veterinary medicine and science    December 30, 2025   Volume 12, Issue 1 e70739 doi: 10.1002/vms3.70739
Key K, Berg K, Kirkegaard J, Andresen KR, Hansen SS.Equine lameness diagnosis largely relies on subjective visual assessments, which can be biased. Although marker-based methods, force plates and inertial measurement units (IMUs) provide objective measurements, they require specialized setups. Vision-based algorithms offer a portable, markerless alternative, but their accuracy needs thorough testing. Objective: To evaluate a custom vision-based algorithm for estimating the groundline across multiple camera angles, including handheld use in horses trotting on a treadmill. Methods: Experimental comparative study. Methods: Eight Standardbred trott...
A modern AI framework integrating deep imputation, synthetic data balancing, and explainable modeling for survival prediction in horse colic.
Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft    December 4, 2025   Volume 264 152767 doi: 10.1016/j.aanat.2025.152767
Ozger ZB, Cihan P, Ozaydin I.Artificial intelligence (AI) has emerged as one of the most transformative tools for developing clinical decision-support systems in veterinary medicine. Despite its growing use, its full potential remains underutilized in equine medicine, an area of both high economic and clinical importance. Accurate survival prediction in horses with colic is crucial for timely intervention and improved clinical outcomes. Methods: This study aimed to predict survival outcomes in horse colic cases by developing models that combine traditional machine-learning algorithms (XGBoost, Light Gradient Boosting Mach...
Statistical approaches for estimating forelimb ground reaction forces in foals during walking and trotting.
Journal of biomechanics    November 16, 2025   Volume 194 113078 doi: 10.1016/j.jbiomech.2025.113078
Opolz MD, Sipes GC, Moshage SG, McCoy AM, Kersh ME.Equine models are useful in biomechanics research due to their similarity in musculoskeletal tissue to humans, their athletic nature, and rapid skeletal development which permits ontogenetic studies. However, a continuing challenge in musculoskeletal models for large animal biomechanics is measuring the ground reaction force (GRF) during locomotion and therefore few reports of biomechanical measures such as joint torques. Here we evaluate two statistical approaches for estimating forelimb ground reaction forces in foals (n = 3). Longitudinal motion capture, GRF, and subject mass data during ...
Lectin Microarray-based Glycomics and Machine Learning Identify Shared Osteoarthritis Biomarkers in Humans, Dogs, and Horses.
bioRxiv : the preprint server for biology    October 17, 2025   2025.10.16.682971 doi: 10.1101/2025.10.16.682971
Peralta AG, Raeisimakiani P, Hayashi K, Mahal LK, Reesink HL.Post-traumatic osteoarthritis (PTOA) is a common sequela to joint injury in both humans and companion animal species such as horses and dogs. Despite the increasing prevalence of osteoarthritis (OA) in humans, investigation of glycosylation changes associated with OA remains in its infancy. Recent advances, such as lectin microarray analysis, now enable detailed glycan profiling in complex biofluids such as synovial fluid. Using lectin microarray technology, this study characterized glycosylation patterns in synovial fluid samples from healthy and OA-affected joints in horses, dogs, and humans...
Deep learning approach for classifying grazing behavior in yearling horses using triaxial accelerometer data: A pilot study.
Journal of equine veterinary science    October 1, 2025   Volume 155 105706 doi: 10.1016/j.jevs.2025.105706
Kamiya U, Kakiuchi K, Kawamura K, Ueda K, Kawai M, Matsui A, Negishi N.Accurate monitoring of grazing behavior in horses is essential for pasture management and welfare evaluation; however, conventional observation methods are labor-intensive and lack temporal resolution. Objective: This pilot study aimed to develop and validate a deep learning model using jaw-mounted accelerometer data to classify grazing and non-grazing behaviors in yearling horses under various pasture conditions. Methods: Four yearling Thoroughbred horses were equipped with triaxle accelerometers mounted under their jaws. Data were recorded at 10 Hz (100 ms) during a 19 h free-grazing period ...
Artificial intelligence in smartphone video analysis for equine asthma diagnostic support.
Equine veterinary journal    July 21, 2025   doi: 10.1111/evj.14559
Gomes C, Coheur L, Tilley P.Equine asthma is a prevalent respiratory disease that negatively impacts horses' health and athletic performance. Traditional diagnostic methods are invasive and require specialised equipment. There is a need for a non-invasive, cost-effective screening tool that can be used by veterinarians and horse handlers in ambulatory settings. Objective: To assess the willingness of veterinarians and horse handlers to adopt such a tool (Questionnaire 1) and the challenges associated with visually recognising equine asthma (Questionnaire 2) and to develop EquiBreathe, an artificial intelligence (AI)-powe...
Artificial intelligence tools to assess different levels of activity performed by semi-wild horses in grassland ecosystems.
Environmental monitoring and assessment    July 16, 2025   Volume 197, Issue 8 922 doi: 10.1007/s10661-025-14363-1
Chodkiewicz A, Prończuk M, Studnicki M.In order to understand the role of horses in ecosystems and to effectively use their grazing in the protection of grasslands, it is important to assess where they primarily stay, followed by whether these habitats are used for grazing or resting. The main goal of the study was the model development based on artificial intelligence tools which allow to distinguish the basic levels of activity performed by horses using data from an accelerometer mounted in a collar worn by animals. The model calibration was based on direct observations of five randomly selected Polish primitive horse mares. In o...
Analytical Data Review on an Artificial Intelligence Platform for Doping Control in Horse Racing.
Analytical chemistry    June 10, 2025   doi: 10.1021/acs.analchem.5c00510
Lai CS, Wong ASY, Wong KS, Wan TSM, Ho ENM.In the screening of prohibited substances (PS) in horse biological samples with gas chromatography/mass spectrometry (GC/MS) and liquid chromatography/mass spectrometry (LC/MS) for doping control, an enormous number of chromatograms are generated. Reviewing these chromatograms to identify suspicious findings requires an extensive manual effort. Recent advancements in Artificial Intelligence (AI) enable its use to classify images into different categories. This can potentially be utilized to perform first-line analysis of chromatograms, which are usually displayed as images, by classifying them...
Integration of machine learning and viscoelastic testing to improve survival prediction in horses experiencing acute abdominal pain at a veterinary teaching hospital.
Equine veterinary journal    April 24, 2025   doi: 10.1111/evj.14517
Macleod BM, Wilkins PA, McCoy AM, Bishop RC.Viscoelastic coagulation testing (VCT) identifies subclinical disruption of coagulation homeostasis and may improve prognostication, particularly for patients with severe systemic inflammation or shock. Machine learning (ML) algorithms may capture complex relationships between clinical variables better than linear regression (GLM). Objective: To evaluate the utility of ML models incorporating VCT and clinical data to predict survival outcomes in horses with acute abdominal pain. Methods: Retrospective observational cohort study. Methods: VCT (VCM Vet™) was performed on 57 horses with acute a...
Transcriptomic Biomarkers in Blood Indicative of the Administration of Recombinant Human Erythropoietin to Thoroughbred Horses.
Drug testing and analysis    April 21, 2025   doi: 10.1002/dta.3899
Cheung HW, Wong KS, Cheng PCF, Tsang CYN, Farrington AF, Wan TSM, Ho ENM.Erythropoiesis-stimulating agents (ESAs) continue to be a significant threat to the integrity of human and equine sports. Besides conventional direct testing, monitoring the biomarkers associated with the effects of ESAs may provide a complementary approach via indirect detection to enhance doping control. In this study, we applied RNA-sequencing (RNA-seq) to discover blood RNA biomarkers in Thoroughbred horses after administration with a long-acting form of recombinant human erythropoietin (rhEPO), methoxy polyethylene glycol epoetin beta, Mircera®. A single subcutaneous administration of Mi...
Exploring equine behavior: Wearable sensors data and explainable AI for enhanced classification.
Journal of equine veterinary science    April 10, 2025   Volume 149 105568 doi: 10.1016/j.jevs.2025.105568
Cetintav B, Yalcin A.Understanding equine behavior through advanced monitoring technologies is crucial for improving animal welfare, optimizing training strategies, and enabling early detection of health or stress-related issues. This study integrates wearable sensor data with Explainable Artificial Intelligence (XAI) techniques, particularly SHAP (Shapley Additive Explanations), to enhance interpretability in equine behavior classification. The data used in this study were sourced from an open-source dataset, ensuring transparency and reproducibility. Orginally, data were collected from 18 horses using sensor dev...
Selection of density standard and X-ray tube settings for computed digital absorptiometry in horses using the k-means clustering algorithm.
BMC veterinary research    March 13, 2025   Volume 21, Issue 1 165 doi: 10.1186/s12917-025-04591-5
Turek B, Pawlikowski M, Jankowski K, Borowska M, Skierbiszewska K, Jasiński T, Domino M.In veterinary medicine, conventional radiography is the first-choice method for most diagnostic imaging applications in both small animal and equine practice. One direction in its development is the integration of bone density evaluation and artificial intelligence-assisted clinical decision-making, which is expected to enhance and streamline veterinarians' daily practices. One such decision-support method is k-means clustering, a machine learning and data mining technique that can be used clinically to classify radiographic signs into healthy or affected clusters. The study aims to investigat...
Detection of mare parturition through balanced multi-scale feature fusion based on improved Libra RCNN.
PloS one    March 4, 2025   Volume 20, Issue 3 e0318498 doi: 10.1371/journal.pone.0318498
Wang B, Duan W, Zhao J, Bai D.Once a mare experiences parturition abnormalities, the outcome between a live foal and a stillborn can change rapidly. Automated detection of mare parturition and timely human intervention is crucial to reducing risks during mare and foal parturition. This paper addresses the challenges of manual monitoring of parturition in large-scale equine facilities due to the unpredictability of mare parturition timing, proposing an algorithm for detecting mare parturition through a balanced multi-scale feature fusion based on an improved Libra RCNN. Initially, a ResNet101 backbone network incorporating ...
Correction: Automated recognition of emotional states of horses from facial expressions.
PloS one    February 12, 2025   Volume 20, Issue 2 e0319501 doi: 10.1371/journal.pone.0319501
Feighelstein M, Ricci-Bonot C, Hasan H, Weinberg H, Rettig T, Segal M, Distelfeld T, Shimshoni I, Mills DS, Zamansky A.[This corrects the article DOI: 10.1371/journal.pone.0302893.].
Discrimination of the Lame Limb in Horses Using a Machine Learning Method (Support Vector Machine) Based on Asymmetry Indices Measured by the EQUISYM System.
Sensors (Basel, Switzerland)    February 12, 2025   Volume 25, Issue 4 1095 doi: 10.3390/s25041095
Poizat E, Gérard M, Macaire C, De Azevedo E, Denoix JM, Coudry V, Jacquet S, Bertoni L, Tallaj A, Audigié F, Hatrisse C, Hébert C, Martin P....Lameness detection in horses is a critical challenge in equine veterinary practice, particularly when symptoms are mild. This study aimed to develop a predictive system using a support vector machine (SVM) to identify the affected limb in horses trotting in a straight line. The system analyzed data from inertial measurement units (IMUs) placed on the horse's head, withers, and pelvis, using variables such as vertical displacement and retraction angles. A total of 287 horses were included, with 256 showing single-limb lameness and 31 classified as sound. The model achieved an overall accuracy o...
Convolutional Neural Networks Assisted Peak Classification in Targeted LC-HRMS/MS for Equine Doping Control Screening Analyses.
Analytical chemistry    February 3, 2025   Volume 97, Issue 6 3236-3241 doi: 10.1021/acs.analchem.4c03608
Barnabé A, Delcourt V, Loup B, Montanuy W, Trévisiol S, Popot MA, Garcia P, Bailly-Chouriberry L.Doping control screening analyses usually involve visual inspection of extracted ion chromatograms (EIC) by a trained analytical chemist, followed by further investigations if needed. This task is both highly repetitive and time-consuming, given the hundreds of compounds and metabolites to be screened in tens of thousands of samples per year. With the recent widespread adoption of machine learning in analytical chemistry and the training of high-performance convolutional neural networks (CNN), these operations can be automated with high accuracy and throughput. Applying this technology to dopi...
Identifying Novel Emotions and Wellbeing of Horses from Videos Through Unsupervised Learning.
Sensors (Basel, Switzerland)    January 31, 2025   Volume 25, Issue 3 doi: 10.3390/s25030859
Bhave A, Kieson E, Hafner A, Gloor PA.This research applies unsupervised learning on a large original dataset of horses in the wild to identify previously unidentified horse emotions. We construct a novel, high-quality, diverse dataset of 3929 images consisting of five wild horse breeds worldwide at different geographical locations. We base our analysis on the seven Panksepp emotions of mammals "Exploring", "Sadness", "Playing", "Rage", "Fear", "Affectionate" and "Lust", along with one additional emotion "Pain" which has been shown to be highly relevant for horses. We apply the contrastive learning framework MoCo (Momentum Contras...
A machine learning approach to identify stride characteristics predictive of musculoskeletal injury, enforced rest and retirement in Thoroughbred racehorses.
Scientific reports    November 22, 2024   Volume 14, Issue 1 28967 doi: 10.1038/s41598-024-79071-1
Bogossian PM, Nattala U, Wong ASM, Morrice-West AV, Zhang GZ, Rana P, Whitton RC, Hitchens PL.Decreasing speed and stride length over successive races have been shown to be associated with musculoskeletal injury (MSI) in racehorses, demonstrating the potential for early detection of MSI through longitudinal monitoring of changes in stride characteristics. A machine learning (ML) approach for early detection of MSI, enforced rest, and retirement events using this same horse-level, race-level, and stride characteristic data across all race sectionals was investigated. A CatBoost model using features from the two races prior to an event had the highest classification performance (sensitiv...
Administration Route Differentiation of Altrenogest via the Metabolomic LC-HRMS Analysis of Equine Urine.
Molecules (Basel, Switzerland)    October 22, 2024   Volume 29, Issue 21 4988 doi: 10.3390/molecules29214988
Elbourne M, Keledjian J, Cawley A, Fu S.Altrenogest, also known as allyltrenbolone, is a synthetic form of progesterone used therapeutically to suppress unwanted symptoms of estrus in female horses. Altrenogest affects the system by decreasing levels of endogenous gonadotrophin and luteinizing and follicle-stimulating hormones, which in turn decreases estrogen and mimics the increase of progesterone production. This results in more manageable mares for training and competition alongside male horses while improving the workplace safety of riders and handlers. However, when altrenogest is administered, prohibited steroid impurities su...
Supervised Machine Learning Techniques for Breeding Value Prediction in Horses: An Example Using Gait Visual Scores.
Animals : an open access journal from MDPI    September 20, 2024   Volume 14, Issue 18 doi: 10.3390/ani14182723
Bussiman F, Alves AAC, Richter J, Hidalgo J, Veroneze R, Oliveira T.Gait scores are widely used in the genetic evaluation of horses. However, the nature of such measurement may limit genetic progress since there is subjectivity in phenotypic information. This study aimed to assess the application of machine learning techniques in the prediction of breeding values for five visual gait scores in Campolina horses: dissociation, comfort, style, regularity, and development. The dataset contained over 5000 phenotypic records with 107,951 horses (14 generations) in the pedigree. A fixed model was used to estimate least-square solutions for fixed effects and adjusted ...
Explainable machine learning for assessing upper respiratory tract of racehorses from endoscopy videos.
Computers in biology and medicine    August 21, 2024   Volume 181 109030 doi: 10.1016/j.compbiomed.2024.109030
Tahir AM, Guo L, Ward RK, Yu X, Rideout A, Hore M, Wang ZJ.Laryngeal hemiplegia (LH) is a major upper respiratory tract (URT) complication in racehorses. Endoscopy imaging of horse throat is a gold standard for URT assessment. However, current manual assessment faces several challenges, stemming from the poor quality of endoscopy videos and subjectivity of manual grading. To overcome such limitations, we propose an explainable machine learning (ML)-based solution for efficient URT assessment. Specifically, a cascaded YOLOv8 architecture is utilized to segment the key semantic regions and landmarks per frame. Several spatiotemporal features are then ex...
From facial expressions to algorithms: a narrative review of animal pain recognition technologies.
Frontiers in veterinary science    July 17, 2024   Volume 11 1436795 doi: 10.3389/fvets.2024.1436795
Chiavaccini L, Gupta A, Chiavaccini G.Facial expressions are essential for communication and emotional expression across species. Despite the improvements brought by tools like the Horse Grimace Scale (HGS) in pain recognition in horses, their reliance on human identification of characteristic traits presents drawbacks such as subjectivity, training requirements, costs, and potential bias. Despite these challenges, the development of facial expression pain scales for animals has been making strides. To address these limitations, Automated Pain Recognition (APR) powered by Artificial Intelligence (AI) offers a promising advancement...
Automated recognition of emotional states of horses from facial expressions.
PloS one    July 15, 2024   Volume 19, Issue 7 e0302893 doi: 10.1371/journal.pone.0302893
Feighelstein M, Riccie-Bonot C, Hasan H, Weinberg H, Rettig T, Segal M, Distelfeld T, Shimshoni I, Mills DS, Zamansky A.Animal affective computing is an emerging new field, which has so far mainly focused on pain, while other emotional states remain uncharted territories, especially in horses. This study is the first to develop AI models to automatically recognize horse emotional states from facial expressions using data collected in a controlled experiment. We explore two types of pipelines: a deep learning one which takes as input video footage, and a machine learning one which takes as input EquiFACS annotations. The former outperforms the latter, with 76% accuracy in separating between four emotional states...
Muscarinic acetylcholine receptors M2 are upregulated in the atrioventricular nodal tract in horses with a high burden of second-degree atrioventricular block.
Frontiers in cardiovascular medicine    November 16, 2023   Volume 10 1102164 doi: 10.3389/fcvm.2023.1102164
Nissen SD, Saljic A, Carstensen H, Braunstein TH, Hesselkilde EM, Kjeldsen ST, Hopster-Iversen C, D'Souza A, Jespersen T, Buhl R.Second-degree atrioventricular (AV) block at rest is very common in horses. The underlying molecular mechanisms are unexplored, but commonly attributed to high vagal tone. Unassigned: To assess whether AV block in horses is due to altered expression of the effectors of vagal signalling in the AV node, with specific emphasis on the muscarinic acetylcholine receptor (M) and the G protein-gated inwardly rectifying K (GIRK4) channel that mediates the cardiac current. Unassigned: Eighteen horses with a low burden of second-degree AV block (median 8 block per 20 h, IQR: 32 per 20 h) were assign...
Fusion of visible and thermal images improves automated detection and classification of animals for drone surveys.
Scientific reports    June 27, 2023   Volume 13, Issue 1 10385 doi: 10.1038/s41598-023-37295-7
Krishnan BS, Jones LR, Elmore JA, Samiappan S, Evans KO, Pfeiffer MB, Blackwell BF, Iglay RB.Visible and thermal images acquired from drones (unoccupied aircraft systems) have substantially improved animal monitoring. Combining complementary information from both image types provides a powerful approach for automating detection and classification of multiple animal species to augment drone surveys. We compared eight image fusion methods using thermal and visible drone images combined with two supervised deep learning models, to evaluate the detection and classification of white-tailed deer (Odocoileus virginianus), domestic cow (Bos taurus), and domestic horse (Equus caballus). We cla...
Machine Learning-Based Sensor Data Fusion for Animal Monitoring: Scoping Review.
Sensors (Basel, Switzerland)    June 20, 2023   Volume 23, Issue 12 5732 doi: 10.3390/s23125732
Aguilar-Lazcano CA, Espinosa-Curiel IE, Ríos-Martínez JA, Madera-Ramírez FA, Pérez-Espinosa H.The development of technology, such as the Internet of Things and artificial intelligence, has significantly advanced many fields of study. Animal research is no exception, as these technologies have enabled data collection through various sensing devices. Advanced computer systems equipped with artificial intelligence capabilities can process these data, allowing researchers to identify significant behaviors related to the detection of illnesses, discerning the emotional state of the animals, and even recognizing individual animal identities. This review includes articles in the English langu...
Load Balancing Using Artificial Intelligence for Cloud-Enabled Internet of Everything in Healthcare Domain.
Sensors (Basel, Switzerland)    June 5, 2023   Volume 23, Issue 11 5349 doi: 10.3390/s23115349
Aqeel I, Khormi IM, Khan SB, Shuaib M, Almusharraf A, Alam S, Alkhaldi NA.The emergence of the Internet of Things (IoT) and its subsequent evolution into the Internet of Everything (IoE) is a result of the rapid growth of information and communication technologies (ICT). However, implementing these technologies comes with certain obstacles, such as the limited availability of energy resources and processing power. Consequently, there is a need for energy-efficient and intelligent load-balancing models, particularly in healthcare, where real-time applications generate large volumes of data. This paper proposes a novel, energy-aware artificial intelligence (AI)-based ...
Detecting fatigue of sport horses with biomechanical gait features using inertial sensors.
PloS one    April 14, 2023   Volume 18, Issue 4 e0284554 doi: 10.1371/journal.pone.0284554
Darbandi H, Munsters C, Parmentier J, Havinga P.Detection of fatigue helps prevent injuries and optimize the performance of horses. Previous studies tried to determine fatigue using physiological parameters. However, measuring the physiological parameters, e.g., plasma lactate, is invasive and can be affected by different factors. In addition, the measurement cannot be done automatically and requires a veterinarian for sample collection. This study investigated the possibility of detecting fatigue non-invasively using a minimum number of body-mounted inertial sensors. Using the inertial sensors, sixty sport horses were measured during walk ...
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