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Topic:Noninvasive Procedures

Noninvasive procedures in horses refer to diagnostic and therapeutic techniques that do not require incision into the body or removal of tissue. These procedures are designed to minimize discomfort and risk to the animal while providing valuable information for veterinary care. Common noninvasive methods include ultrasonography, thermography, and endoscopy, which allow for the examination of internal structures without surgery. Additionally, techniques such as laser therapy and extracorporeal shock wave therapy are employed for treatment purposes. These procedures are beneficial for assessing and managing a variety of conditions, including musculoskeletal disorders and soft tissue injuries. This page compiles peer-reviewed research studies and scholarly articles that explore the application, effectiveness, and advancements of noninvasive procedures in equine medicine.
Ultrasound-guided cervical centesis to obtain cerebrospinal fluid in the standing horse. Pease A, Behan A, Bohart G.Horses with intracranial lesions and severe ataxia are not good anesthesia candidates; however, only one method to obtain cerebrospinal fluid (CSF) from the cervical region in a standing horse has been reported. This method is not performed routinely due to the difficulty for sample acquisition. Our hypothesis is that standing cervical centesis can be performed in horses without complication. Ultrasound-guided centesis of the CSF between C1 and C2 in 11 clinically normal horses and two horses with neurologic signs were performed. Horses were sedated and ultrasound was used to identify the suba...
Application of the lactose 13C-ureide breath test for measurement of equine orocaecal transit time.
Equine veterinary journal. Supplement    August 4, 2011   Issue 39 49-55 doi: 10.1111/j.2042-3306.2011.00407.x
Sutton DG, Preston T, Love S.Application of the lactose (13) C-ureide breath test (LUBT) for measurement of equine orocaecal transit time (OCTT) has not been reported previously. The ability to assess OCTT noninvasively, and to investigate its relationship to gastric emptying rate and small intestinal transit, would be of both clinical and research value. Objective: 1) Assessment of the LUBT in healthy horses, with comparison of induced versus noninduced test protocols. 2) Application of a new dual stable isotope breath test (lactose (13) C-ureide and (13) C-octanoic acid) for gastrointestinal transit measurement. Objecti...
In vitro validation of the lactose 13C-ureide breath test for equine orocaecal transit time measurement.
Equine veterinary journal. Supplement    August 4, 2011   Issue 39 42-48 doi: 10.1111/j.2042-3306.2011.00406.x
Sutton DG, Preston T, Love S.Validation of a reliable, noninvasive clinical test for quantification of equine orocaecal transit time (OCTT) is required. This would facilitate an evidence-based approach to investigation and treatment of equine small intestinal disorders. Objective: 1) Comparison of the lactose (13) C-ureide breath test (LUBT) with the hydrogen breath test (H(2) BT) for OCTT measurement. 2) Identification of the characteristics of gastrointestinal microbial glycosylureide hydrolase activity in vitro. 3) Production of an optimised protocol for the LUBT for in vivo measurement of equine OCTT. Objective: Signi...
Effect of topical vapocoolant spray on response to arthrocentesis and intravenous catheterization in unsedated horses.
American journal of veterinary research    June 2, 2011   Volume 72, Issue 6 746-750 doi: 10.2460/ajvr.72.6.746
Fjordbakk CT, Haga HA.To assess the efficacy of a commercially available topical vapocoolant spray in reducing responses to arthrocentesis of the middle carpal (MC) and metacarpophalangeal (MCP) joints and jugular vein catheterization in unsedated horses. Methods: 8 healthy research horses. Methods: Arthrocentesis of both MC and MCP joints and bilateral jugular vein catheterization were performed in each horse. Immediately prior to skin penetration, 1 randomly selected MC joint, MCP joint, and jugular vein were sprayed with a vapocoolant liquid (intervention product), and the contralateral MC joint, MCP joint, and ...
Technical note: a 2-stage cecal cannulation technique in standing horses.
Journal of animal science    March 18, 2011   Volume 89, Issue 8 2425-2429 doi: 10.2527/jas.2010-3718
Beard WL, Slough TL, Gunkel CD.Cecal cannulation is necessary for sampling of intestinal contents for a variety of nutritional or digestive physiology studies. This report describes a 2-stage technique for permanent cecal cannulation in standing horses. For the first procedure, a right flank laparotomy is performed and a small pouch of the cecal base exteriorized and sutured to the body wall. The second procedure is performed approximately 1 wk later. During the second procedure, the exposed cecal pouch is removed and the cannula inserted. Ten horses were cannulated using this technique. After the first procedure, 1 horse d...
Repetitive stimulation of the common peroneal nerve as a diagnostic aid for botulism in foals.
Journal of veterinary internal medicine    February 11, 2011   Volume 25, Issue 2 365-372 doi: 10.1111/j.1939-1676.2011.0682.x
Aleman M, Williams DC, Jorge NE, Magdesian KG, Brosnan RJ, Feary DJ, Hilton HG, Kozikowski TA, Higgins JK, Madigan JE, Lecouteur RA.Botulism is a potentially fatal paralytic disorder for which definitive diagnosis is difficult. Objective: To determine if repetitive stimulation of the common peroneal nerve will aid in the diagnosis of botulism in foals. Methods: Four control and 3 affected foals. Methods: Validation of the test in healthy foals for its comparison in foals with suspected botulism. Controls were anesthetized and affected foals were sedated to avoid risks of anesthesia. The common peroneal nerve was chosen for its superficial location and easy access. Stimulating electrodes were placed along the common peronea...
Assessment of the impact of collection temperature and sampler design on the measurement of exhaled breath condensate pH in healthy horses.
Veterinary journal (London, England : 1997)    January 19, 2011   Volume 191, Issue 2 208-212 doi: 10.1016/j.tvjl.2010.12.013
Whittaker AG, Love S, Parkin TD, Duz M, Cathcart M, Hughes KJ.The pH measurement of exhaled breath condensate (EBC) may provide a non-invasive method of assessing the lower airways of horses but the methodology used may influence findings. The aim of this study was to investigate the effect of two sampling devices and three methods of condensation surface cooling (ethanol slush, -100°C; dry ice, -75°C; water ice, 0°C) on EBC pH. Each method was tested 30 times using six healthy ponies. Sample pH was determined before and after de-aeration with argon for 10 min. Sampler design was found to significantly affect pH. Samples collected as a liquid had a si...
Echocardiographic changes in heart size in hypohydrated horses.
Journal of veterinary internal medicine    October 12, 2010   Volume 25, Issue 3 563-569 doi: 10.1111/j.1939-1676.2010.0612.x
Underwood C, Norton JL, Nolen-Walston RD, Dallap-Schaer BL, Boston R, Slack J.Hypohydration causes transient echocardiographic changes in pigs, dogs, humans, and cats. These changes mask the diagnosis of some cardiac diseases (valvular regurgitation, dilated cardiomyopathy) and promote the diagnosis of others (hypertropic cardiomyopathy and infiltrative disease), thus inhibiting accurate echocardiographic evaluation. Objective: To describe the echocardiographic changes associated with hypohydration in normal horses. Methods: Ten adult horses without detectable cardiac disease. Methods: Experimental study. Echocardiographic examinations were performed on horses in the eu...
A linear laser scanner to measure cross-sectional shape and area of biological specimens during mechanical testing.
Journal of biomechanical engineering    October 5, 2010   Volume 132, Issue 10 105001 doi: 10.1115/1.4002374
Vergari C, Pourcelot P, Holden L, Ravary-Plumioën B, Laugier P, Mitton D, Crevier-Denoix N.Measure of the cross-sectional area (CSA) of biological specimens is a primary concern for many biomechanical tests. Different procedures are presented in literature but besides the fact that noncontact techniques are required during mechanical testing, most of these procedures lack accuracy or speed. Moreover, they often require a precise positioning of the specimen, which is not always feasible, and do not enable the measure of the same section during tension. The objective of this study was to design a noncontact, fast, and accurate device capable of acquiring CSA of specimens mounted on a ...
Comparison between blood serum and salivary cortisol concentrations in horses using an adrenocorticotropic hormone challenge.
Equine veterinary journal    September 29, 2010   Volume 43, Issue 4 487-493 doi: 10.1111/j.2042-3306.2010.00294.x
Peeters M, Sulon J, Beckers JF, Ledoux D, Vandenheede M.In horses, serum cortisol concentration is considered to provide an indirect measurement of stress. However, it includes both free and bound fractions. The sampling method is also invasive and often stressful. This is not the case for salivary cortisol, which is collected using a more welfare-friendly method and represents a part of the free cortisol fraction, which is the biologically active form. Objective: To compare salivary and serum cortisol assays in horses, in a wide range of concentrations, using an adrenocorticotropic hormone (ACTH) stimulation test, in order to validate salivary cor...
Maggot debridement therapy for laminitis.
The Veterinary clinics of North America. Equine practice    August 12, 2010   Volume 26, Issue 2 447-450 doi: 10.1016/j.cveq.2010.06.002
Morrison S.Maggot debridement therapy is a nontraumatic, minimally invasive method to treat infections in a foot compromised by chronic laminitis. A mechanical strategy must first be in place to address the instability of the distal phalanx and hoof capsule. Adverse reactions to maggot debridement therapy are uncommon and the only side effect observed has been irritation or hypersensitivity at the site. Chronic laminitic cases of sepsis/necrosis within the hoof benefit from this procedure due to the noninvasive, continuous debridement and healing properties provided by the larvae.
Bioelectrical impedance analysis determination of water content and distribution in the horse.
Research in veterinary science    August 8, 2010   Volume 90, Issue 3 516-520 doi: 10.1016/j.rvsc.2010.07.012
Latman NS, Keith N, Nicholson A, Davis M.A horse's hydration status is critical to its health. The accurate and quantitative determination of it has been problematic because of size, length and density of hair, and uneven topography. The objective of this study was to validate a bioelectrical impedance analysis (BIA) method for objectively quantifying hydration status. Monofrequency BIA values and simple biometric measurements were used to construct predictive equations for total body water, plasma, extracellular, and intra-cellular fluid volumes. These predictive equations were correlated with standard body fluid dilution reference ...
Stabling causes a significant increase in the pH of the equine airway.
Equine veterinary journal    April 14, 2010   Volume 41, Issue 9 940-943 doi: 10.2746/042516409x474383
Whittaker AG, Love S, Parkin TD, Duz M, Hughes KJ.Regulation of pH homeostasis in the equine lung is poorly understood. Measurement of exhaled breath condensate (EBC) pH provided a simple, highly repeatable and noninvasive method for the longitudinal investigation of changes in airway pH in response to environmental changes. Stabling of horses was found to lead to a small (approximately 100-200 parts/billion) but significant (P < 0.001) increase in ambient ammonia concentration when compared to pasture. This increase in exposure to ambient ammonia concentration was associated with significant (P = 0.002) increases in EBC pH and exhaled amm...
Use of quantitative ultrasonography for noninvasive surveillance of the third metacarpal bone in racing and training Thoroughbreds.
American journal of veterinary research    December 3, 2009   Volume 70, Issue 12 1484-1493 doi: 10.2460/ajvr.70.12.1484
Tabar-Rodriguez JJ, Cruz AM, Monteith G, Gordon K, Hurtig MB.OBJECTIVE-To use quantitative ultrasonography to evaluate the association between the speed of sound (SOS) at 9 sites in the third metacarpal bone (MCIII) of racing Thoroughbreds with workload accumulation and the effect that MCIII failure has on this association. ANIMALS-Sixty-two 2- and 3-year-old Thoroughbreds in racing condition. PROCEDURES-Cumulative work index (CWI) was used to calculate total workload (CWI(total)) and also 3 independent CWIs for the various gaits (ie, trot [CWI(trot)], gallop [CWI(gallop)], and race [CWI(race)]) used during training and racing. Speed of sound was monito...
In vivo confocal microscopy of the normal equine cornea and limbus.
Veterinary ophthalmology    November 26, 2009   Volume 12 Suppl 1 57-64 doi: 10.1111/j.1463-5224.2009.00730.x
Ledbetter EC, Scarlett JM.To describe morphologic features, pachymetry and endothelial cell density of the normal equine cornea and limbus by in vivo confocal microscopy. Methods: Ten horses without ocular disease. Methods: The central and peripheral corneas were examined with a modified Heidelberg Retina Tomograph II and Rostock Cornea Module using a combination of automated and manual image acquisition modes. Thickness measurements of various corneal layers were performed and endothelial cell density determined. Results: Images of the constituent cellular and noncellular elements of the corneal epithelium, stroma, en...
A non-invasive technique for standing surgical repair of urinary bladder rupture in a post-partum mare: a case report.
Irish veterinary journal    November 1, 2009   Volume 62, Issue 11 734-736 doi: 10.1186/2046-0481-62-11-734
Stephen J, Harty M, Hollis A, Yeomans J, Corley K.An 11-year-old mare presented 36 hours after foaling with a ruptured bladder. Uroperitoneum was diagnosed on ultrasound and from the creatinine concentration of the peritoneal fluid. Bladder endoscopy demonstrated tissue necrosis and a rent in the dorsocranial aspect of the bladder. Following stabilisation, including abdominal drainage and lavage, the mare was taken to standing surgery. Under continuous sedation and epidural anaesthesia, and after surgical preparation, a Balfour retractor was placed in the vagina. Using sterile lubricant and moderate force, it was possible to insert a hand int...
Methods and reliability of tissue Doppler imaging for assessment of left ventricular radial wall motion in horses.
Journal of veterinary internal medicine    August 4, 2009   Volume 23, Issue 3 643-652 doi: 10.1111/j.1939-1676.2009.0287.x
Schwarzwald CC, Schober KE, Bonagura JD.Noninvasive assessment of left ventricular (LV) function is incompletely studied in horses. Objective: The goals of this study were to investigate the feasibility, techniques, and reliability of tissue Doppler imaging (TDI) for characterization of LV radial wall motion in healthy horses. Methods: Three Standardbreds, 3 Thoroughbreds; age 8-14 years; body weight 517-606 kg. Methods: Repeated echocardiographic examinations were performed by 2 observers in unsedated horses using TDI. Test reliability was determined by estimating measurement variability, within-day interobserver variability, and b...
Reproducibility of a non-invasive ultrasonic technique of tendon force measurement, determined in vitro in equine superficial digital flexor tendons.
Journal of biomechanics    July 31, 2009   Volume 42, Issue 13 2210-2213 doi: 10.1016/j.jbiomech.2009.06.005
Crevier-Denoix N, Ravary-Plumioën B, Evrard D, Pourcelot P.A non-invasive ultrasonic (US) technique of tendon force measurement has been recently developed. It is based on the relationship demonstrated between the speed of sound (SOS) in a tendon and the traction force applied to it. The objectives of the present study were to evaluate the variability of this non-linear relationship among 7 equine superficial digital flexor (SDF) tendons, and the reproducibility of SOS measurements in these tendons over successive loading cycles and tests. Seven SDF tendons were equipped with an US probe (1MHz), secured in contact with the skin overlying the tendon me...
Influence of subclinical inflammatory airway disease on equine respiratory function evaluated by impulse oscillometry.
Equine veterinary journal    July 1, 2009   Volume 41, Issue 4 384-389 doi: 10.2746/042516409x366121
Richard EA, Fortier GD, Denoix JM, Art T, Lekeux PM, Van Erck E.Inflammatory airway disease (IAD) is a nonseptic condition of the lower respiratory tract. Its negative impact on respiratory function has previously been described using either forced expiration or forced oscillations techniques. However, sedation or drug-induced bronchoconstriction were usually required. The impulse oscillometry system (IOS) is a noninvasive and sensitive respiratory function test validated in horses, which could be useful to evaluate IAD-affected horses without further procedures. Objective: To determine the sensitivity of IOS in detecting alterations of the respiratory fun...
Left ventricular radial and circumferential wall motion analysis in horses using strain, strain rate, and displacement by 2D speckle tracking.
Journal of veterinary internal medicine    June 1, 2009   Volume 23, Issue 4 890-900 doi: 10.1111/j.1939-1676.2009.0321.x
Schwarzwald CC, Schober KE, Berli AS, Bonagura JD.Noninvasive assessment of left-ventricular (LV) function is clinically relevant, but is incompletely studied in horses. Objective: To document the feasibility, describe the techniques, and determine the reliability of 2D speckle tracking (2DST) for characterization of LV radial and circumferential wall motion in horses. Methods: Three Standardbreds, 3 Thoroughbreds; age 8-14 years; body weight 517-606 kg. Methods: Observational study. Repeated 2-dimensional echocardiographic examinations were performed in unsedated horses by 2 observers and subsequently analyzed by 2DST. Test reliability was d...
Assessment of pregnancy in the late-gestation mare using digital infrared thermography.
Theriogenology    May 30, 2009   Volume 72, Issue 3 372-377 doi: 10.1016/j.theriogenology.2009.03.005
Bowers S, Gandy S, Anderson B, Ryan P, Willard S.The objective of this study was to investigate use of digital infrared thermal imaging (DITI) to determine whether surface temperature gradient differences exist between pregnant and nonpregnant mares as a noncontact method to determine pregnancy status. On the day measurements were collected, each pregnant mare (n=10; beginning at 292.4+/-1.4 d of gestation) was paired with a nonpregnant mare (n=17). Ambient temperature, DITI measurements (left and right flank, wither temperatures [i.e., animal surface control] and background temperature), and rectal temperatures were obtained every 7 d for 5...
Influence of track surface on the equine superficial digital flexor tendon loading in two horses at high speed trot.
Equine veterinary journal    May 28, 2009   Volume 41, Issue 3 257-261 doi: 10.2746/042516409x394445
Crevier-Denoix N, Pourcelot P, Ravary B, Robin D, Falala S, Uzel S, Grison AC, Valette JP, Denoix JM, Chateau H.Although track surfaces are a risk factor of tendon injuries, their effects on tendon loading at high speed are unknown. Using a noninvasive ultrasonic technique, it is now possible to evaluate the forces in the superficial digital flexor tendon (SDFT) in exercise conditions. Objective: To compare the effects of an all-weather waxed track (W) vs. a crushed sand track (S), on the SDFT loading in the trotter horse at high speed. Methods: Two trotter horses were equipped with the ultrasonic device (1 MHz ultrasonic probe, fixed on the palmar metacarpal area of the right forelimb). For each trial,...
Rupture of the lateral lobe of the biceps brachii tendon in an Arabian horse.
Veterinary and comparative orthopaedics and traumatology : V.C.O.T    May 19, 2009   Volume 22, Issue 3 253-255 doi: 10.3415/VCOT-08-09-0095
Spadari A, Spinella G, Romagnoli N, Valentini S.Rupture of the lateral lobe of the proximal tendon of the biceps brachii muscle was diagnosed in an Arabian horse. To the authors' knowledge, this is the only report of this condition in horses. Although clinical signs were helpful in the identification of the location of disease, ultrasonographic examination was a more definitive and non- invasive means of diagnosing the pathological condition. Bursoscopic examination of the intertubercular bursa was also useful in obtaining confirmation of the diagnosis, and for debridement and lavage of the bursa.
Online detection of an emotional response of a horse during physical activity.
Veterinary journal (London, England : 1997)    April 17, 2009   Volume 181, Issue 1 38-42 doi: 10.1016/j.tvjl.2009.03.017
Jansen F, Van der Krogt J, Van Loon K, Avezzù V, Guarino M, Quanten S, Berckmans D.The objective of this research was to develop a non-invasive method to detect an emotional response of a horse to novelty during physical activity. Two horses performed 20 trials each, in which the horse's heart rate (HR) and physical activity were continuously measured. The relationship between the horse's physical activity and HR was described by a mathematical model allowing online decomposition of the horse's HR into a physical component and a component containing information about its emotional state. Exposure to the novel object resulted in an increase in the emotional component of HR, w...
A cranial intercondylar arthroscopic approach to the caudal medial femorotibial joint of the horse.
Equine veterinary journal    March 24, 2009   Volume 41, Issue 1 5-10 doi: 10.2746/042516408x347042
Muurlink T, Walmsley J, Young D, Whitton C.Current noninvasive techniques for imaging the soft tissue structures of the stifle have limitations. Arthroscopy is commonly used for the investigation and treatment of stifle pain. Cranial and caudal arthroscopic approaches to the femorotibial joints are used. However, complete examination of the axial aspect of the medial femorotibial joint (MFTJ) is not possible currently. Objective: To develop a cranial approach to the caudal pouch of the MFTJ and to assess whether it would allow a more complete examination of the compartment and facilitate the caudomedial approach. Methods: The regional ...
Detection of virulent Rhodococcus equi in exhaled air samples from naturally infected foals.
Journal of clinical microbiology    January 14, 2009   Volume 47, Issue 3 734-737 doi: 10.1128/JCM.01395-08
Muscatello G, Gilkerson JR, Browning GF.Virulent Rhodococcus equi causes pyogranulomatous bronchopneumonia in foals. The route of infection of foals has been considered to be inhalation of aerosolized bacteria from soil that is contaminated with equine feces. Thus, disease caused by R. equi has been regarded as an opportunistic infection of environmental origin and not a contagious disease. In this study, we report the exhalation of virulent R. equi from the respiratory tract of naturally infected foals. A handheld air-monitoring system was used to recover virulent R. equi from the exhaled breath of foals, and the concentration of v...
The use of oral endoscopy for detection of cheek teeth abnormalities in 300 horses.
Veterinary journal (London, England : 1997)    December 2, 2008   Volume 178, Issue 3 396-404 doi: 10.1016/j.tvjl.2008.09.029
Simhofer H, Griss R, Zetner K.The main objective of this study was to evaluate an endoscopic examination protocol for routine dental examination in horses. The oral cavities of 300 standing, sedated horses were examined under field and hospital conditions with a rigid endoscope using a standardised technique that included examination of the occlusal, lingual (palatal) and buccal surfaces of all cheek teeth rows. The most common cheek teeth abnormalities detected were sharp enamel edges (present in 96.3% of horses), focal overgrowths (64.3%), fissure fractures (54.3%), diastemata (24.3%) and infundibular hypoplasia/caries (...
Chiropractic effectiveness is widely studied in the treatment of human back pain.
Equine veterinary journal    November 26, 2008   Volume 40, Issue 6 619 doi: 10.1111/j.2042-3306.2008.tb01140.x
Moffatt D.No abstract available
Device for unobtrusive drug administration and blood sampling in horses recovering from anaesthesia.
The Veterinary record    September 9, 2008   Volume 163, Issue 10 303-304 doi: 10.1136/vr.163.10.303
Clutton RE.No abstract available
Novel optical imaging technique to determine the 3-D orientation of collagen fibers in cartilage: variable-incidence angle polarization-sensitive optical coherence tomography.
Osteoarthritis and cartilage    July 14, 2008   Volume 17, Issue 1 33-42 doi: 10.1016/j.joca.2008.05.005
Ugryumova N, Jacobs J, Bonesi M, Matcher SJ.To investigate a novel optical method to determine the three dimensional (3-D) structure of articular cartilage collagen non-destructively. Methods: Polarization-sensitive optical coherence tomography was used to determine the apparent optical birefringence of articular cartilage for a number of different illumination directions. A quantitative method based on the theory of light propagation in uniaxial crystalline materials was validated on equine flexor tendon. Qualitative maps of fiber polar and azimuthal orientation at sites on the posterior and anterior segments of the equine third metaca...
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