Analyze Diet

Topic:Observational Study

Observational studies in horses involve systematically assessing and recording behaviors, conditions, or other characteristics without manipulating the study environment. These studies provide insights into natural equine behaviors, health conditions, and interactions with their environment. Researchers often focus on aspects such as herd dynamics, feeding patterns, or responses to environmental changes. The data collected can help identify trends, correlations, and potential risk factors for certain conditions. This page compiles peer-reviewed research studies and scholarly articles that explore various observational methodologies and their applications in understanding equine behavior and health.
An Observational Study of Wood-Chewing in Mares and Their Foals Kept in Fenced Outdoor Paddocks with Variable Browsing Access.
Journal of applied animal welfare science : JAAWS    May 7, 2025   1-16 doi: 10.1080/10888705.2025.2496496
Bouquet A, Nicol C, Díez-León M.The natural behaviour of wood-chewing (WC) in horses may, under certain conditions, become excessive or it may precede crib-biting . However, the potential causes of WC, including frustrated foraging motivation) or developmental exploratory or teething behavior are poorly understood. We studied WC in 31 mares and their foals, examining associations with browsing access, stocking density, foal sex and performance of other non-nutritive oral behaviors. Behaviors (counts/hour) were recorded by all-occurrences sampling during focal, continuous observations of grazing,resting and suckling for 20 ...
Inducing and measuring positive affective state in domesticated equines: A Delphi consultation.
Veterinary journal (London, England : 1997)    May 6, 2025   Volume 312 106370 doi: 10.1016/j.tvjl.2025.106370
Loftus L, Asher L, Leach M.Over the last twenty years the definition of good animal welfare has advanced from the 'absence of negative welfare states' to the aim of identifying the presence of positive welfare states; however, research on positive animal welfare is relatively new. Consequently, through expert consultation, this study aimed to synthesise knowledge regarding domesticated equine emotional state, specifically methods to induce and measure positive affective states, which could be of significant benefit to equine welfare across sectors. A Delphi consultation of experts in the field of equine behaviour, welfa...
Starch Allowance and Muscle Enzyme Activity in Healthy Standardbred Trotters Trained by Professional Trainers.
Journal of animal physiology and animal nutrition    May 6, 2025   doi: 10.1111/jpn.14127
Connysson M, Jansson A.It is generally accepted that plasma muscle enzyme activity of creatine kinase (CK) and aspartate aminotransferase (AST) may increase in racehorses after exercise and racing, indicating muscle fibre damage and/or increased leakage from muscle fibres. However, other studies suggest that starch intake might influence plasma muscle enzyme activity reported postexercise. This study aimed to evaluate the effect of different starch allowances on plasma muscle enzyme activity in Standardbred trotters in professional training. Seventy-six horses from five professional trainers were sampled pre- and po...
Effect of high-speed exercise on subchondral bone in the metacarpo-/metatarsophalangeal joints of 2-year-old Thoroughbred racehorses in their first year of training.
Equine veterinary journal    May 5, 2025   doi: 10.1111/evj.14524
Ciamillo SA, Bills KW, Gassert TM, Richardson DW, Brown KA, Stefanovski D, Ortved KF.Stress-induced bone injury can lead to catastrophic injuries in Thoroughbred racehorses. Accumulation of high-speed exercise can increase the risk of subchondral bone injury. Objective: To investigate the effect of high-speed exercise on the subchondral bone of the metacarpo-metatarsophalangeal (MCP/MTP) joints using standing cone-beam computed tomography (CBCT) in young racing Thoroughbreds. Methods: Observational cohort study. Methods: Forty-one 2-year-old Thoroughbred racehorses during their first year of training were evaluated at 0, 6 and 12 months. Horses were evaluated for lameness an...
Development of a health-related quality-of-life assessment tool for equines with pituitary pars intermedia dysfunction.
Equine veterinary journal    May 2, 2025   doi: 10.1111/evj.14513
Bouquet A, Nicol C, Knowles EJ, Schofield I, Menzies-Gow NJ.Clinical signs of pituitary pars intermedia dysfunction (PPID) are frequently mistaken for 'normal' ageing and may not be optimally assessed. Objective quality of life (QoL) assessment could improve clinical decision-making. Objective: To develop an owner-reported health-related quality-of-life (HRQoL) assessment tool for equines with PPID. To assess factors associated with HRQoL scores. Methods: Quantitative, cross-sectional study. Methods: HRQoL tool development followed a standard psychometric process of item (any aspect of PPID and its management that could impact QoL) identification (foll...
Transfusion of rhodococcal hyperimmune plasma to newborn foals does not markedly alter serum electrolyte or protein concentrations.
Journal of the American Veterinary Medical Association    April 30, 2025   Volume 263, Issue 7 859-865 doi: 10.2460/javma.25.02.0115
Volding DM, Flores-Ahlschwede P, Cramer MJ, Landrock KK, Sayre KC, Welsh TH, Bordin AI, Piccione J, Cohen ND.To determine the effects of transfusion of Rhodococcus equi hyperimmune plasma (REHIP) on serum electrolyte and protein concentrations in neonatal foals. Unassigned: A prospective observational study of 355 privately owned Thoroughbred foals from 13 farms in New York and cross-sectional study of REHIP samples. Blood was collected immediately before and after transfusion of either 1 or 2 L of REHIP to foals, and serum samples were separated and frozen until tested for concentrations of sodium (Na), potassium (K), chloride (Cl), total protein (TP), albumin, and globulins. Samples (n = 90) of REH...
Marker based and markerless motion capture for equestrian rider kinematic analysis: A comparative study.
Journal of biomechanics    April 26, 2025   Volume 186 112728 doi: 10.1016/j.jbiomech.2025.112728
Cameron-Whytock H, Divall H, Lewis M, Apps C.The study hypothesised that a markerless motion capture system can provide kinematic data comparable to a traditional marker-based system for riders mounted on a horse. The objective was to assess the markerless system's accuracy by directly comparing joint and segment angle measurements taken during walking and trotting with those obtained from a marker-based system. Ten healthy adult participants performed five dynamic trials during walking and trotting. A twelve-camera marker-based system and eight-camera 2D video-based system were synchronised. Three-dimensional hip, knee, shoulder and elb...
Official and private animal welfare inspectors’ perception of their own on-site inspections.
Frontiers in veterinary science    April 25, 2025   Volume 12 1575471 doi: 10.3389/fvets.2025.1575471
Lundmark Hedman F, Ewerlöf IR, Frössling J, Berg C.The presence of a trustworthy and effective animal welfare control system is important both for animal welfare and for public and consumer trust. The inspectors' main task, regardless of whether they are official inspectors or private auditors, is to check for and enforce compliance with any relevant regulations. The aim of this study was to investigate how official animal welfare inspectors and private animal welfare auditors in Sweden perceive their inspection work and to explore any differences in the perception of being an inspector between these two groups. An electronic questionnaire was...
Pilot Study: Simultaneous Daily Recording of Total Locomotor Activity and Heart Rate in Horses for Application in Precision Livestock Farming.
Animals : an open access journal from MDPI    April 22, 2025   Volume 15, Issue 9 1189 doi: 10.3390/ani15091189
Aragona F, Rizzo M, Arrigo F, Arfuso F, Fazio F, Giudice E, Pugliatti P, Piccione G, Giannetto C.Among physiological parameters, total locomotor activity (TLA) and heart rate (HR) are used as physiological indicators in animal welfare evaluations. The present study aimed to simultaneously record for 24 h the TLA and HR of ten clinically healthy horses housed in conventional individual boxes subjected to a natural photoperiod and temperature. An actigraphy-based data logger was placed on the headstall, and an equine HR monitor was placed around the chest to monitor TLA and HR, respectively. Activity was monitored with 5 min sampling intervals and HR with 5 s intervals. To make the data poi...
Welfare assessment of racehorses provides a baseline for continued monitoring.
Equine veterinary journal    April 21, 2025   doi: 10.1111/evj.14510
Annan R, Trigg L, Allen K, Hockenhull J, Valenchon M, Mullan S.Racehorse welfare continues to be a focus of stakeholders both within and outside of the industry, and there is a need for evidence-based animal welfare data. Objective: To gather data on racehorse welfare through a combination of animal-based observations and information about management practices, and to ascertain the feasibility of trained industry assessors carrying out a racehorse welfare assessment. Methods: Cross-sectional. Methods: Sixteen trained industry assessors conducted individual observations of 737 racehorses in 21 Flat racing (F), 27 National Hunt (NH) and 26 Dual-purpose (D) ...
Motor pathway evaluation by transcranial magnetic stimulation in Swedish horses with acquired equine polyneuropathy.
Equine veterinary journal    April 21, 2025   doi: 10.1111/evj.14506
May A, Hanche-Olsen S, Goehring LS, Matiasek K, Jäderlund KH, Zablotski Y, Gröndahl G.Acquired equine polyneuropathy in Nordic horses (AEP) is the most prevalent equine polyneuropathy in Norway, Sweden, and Finland and is characterised by pelvic limb knuckling due to metatarsophalangeal extension dysfunction. Objective: To evaluate the function of descending motor pathways in AEP using transcranial magnetic stimulation (TMS). Methods: An analytical, observational cohort design. Methods: Clinical findings and TMS results of 20 horses from an AEP outbreak in Sweden were evaluated at 5-month intervals. Latency time (LT) in milliseconds (ms) between coil discharge and onset of musc...
Agreement between subjective gait assessment and markerless video gait-analysis in endurance horses.
Equine veterinary journal    April 21, 2025   doi: 10.1111/evj.14516
de Chiara M, Montano C, De Matteis A, Guidi L, Buono F, Auletta L, Del Prete C, Pasolini MP.Subjective evaluation of gait by official endurance veterinarians (OEVs) is used to determine 'fitness-to-compete' in horses participating in endurance competitions. Objective gait analysis systems could aid in quick and verifiable judgements. Objective: To assess the agreement between objective analysis of head and pelvis vertical movement asymmetry performed with a markerless artificial intelligence motion tracking system (AI-MTS) and subjective lameness assessment performed by an accredited FEI OEV to judge horse gaits. Methods: Cross-sectional. Methods: During three endurance competitions,...
Performance of a non-contact veterinary infrared thermometer and reference intervals of equine temperature at different body sites.
Frontiers in veterinary science    April 17, 2025   Volume 12 1583839 doi: 10.3389/fvets.2025.1583839
Alberghina D, Tombolani C, Quintavalla F.Evaluating the body temperature of horses () is essential for monitoring their health. Rectal thermometry is the most common method for determining the temperatures of adult horses and foals. However, this method requires restraining the animals for several seconds, and it poses potential risks to both horses and humans. This study compared infrared and rectal thermometry in 126 horses, evaluating age and measurement site influences on body temperature. Horses were divided into three groups: foals (4-12 months), Shetland ponies (8-12 months), and adult horses (2-30 years). Rectal, ocular...
Head and Neck Positions Affect Equine Kinematic Variables in Marcha Batida Gait-A Pilot Study.
Animals : an open access journal from MDPI    April 9, 2025   Volume 15, Issue 8 doi: 10.3390/ani15081090
Martins NA, Fonseca BPA, Silvatti AP, Valente FL, Soares NL, Simonato SP, Rosa LP, Andrade MO, Barcelos KMDC.The Mangalarga Marchador (MM) breed naturally performs four-beat gaits known as "Marcha". Kinematic analysis can provide insights for optimizing training and competition performance in this breed while potentially mitigating welfare concerns associated with extreme head and neck positions (HNPs) applied without an adequate understanding of their impacts. We examined how different HNPs affect the MM horse's Marcha Batida gait. Four HNPs were evaluated: HPN1-loose reins, HPN2-the competition standard for MM, HPN3-an extremely elevated head and neck, and HPN4-a slightly behind-the-vertical positi...
Incidence of complications from perioperative urinary catheterisation in horses.
Australian veterinary journal    April 5, 2025   doi: 10.1111/avj.13445
Quinn CT.Catheterisation of the urinary bladder is a common procedure during perioperative care of horses. Benefits of urinary catheters include a reduction in the risk of recovery-associated slip hazards as a consequence of voided urine and the measurement of urine output during anaesthesia. However, there is little information regarding the risks associated with this procedure. A retrospective cohort study was undertaken in horses admitted to a university teaching hospital for surgery during which a urinary catheter was placed perioperatively. Data were retrieved from clinical records including signa...
Pain Assessment of Horses With Trigeminal-Mediated Headshaking (TMHS) at Rest Between Episodes.
Journal of veterinary internal medicine    April 1, 2025   Volume 39, Issue 3 e70064 doi: 10.1111/jvim.70064
Franzen V, Reisbeck D, Leibl Y, Schoster A, May A.While there is agreement that horses experience pain during the actual headshaking attacks, there is still a lack of research about the time between the individual episodes in this species. Objective: To evaluate the signs of pain in horses with TMHS at rest in the absence of common triggering factors. Methods: Twenty TMHS horses (not head shaking at the time of pain assessment), 20 horses with colic, and 20 clinically healthy horses. Methods: Descriptive study assessing pain in TMHS horses during the absence of clinical signs using the Horse Grimace scale (HGS). To grade the pain shown with T...
Benefits of horseback riding for neurotypical children and adolescents: a scoping review.
CoDAS    March 31, 2025   Volume 37, Issue 3 e20240083 doi: 10.1590/2317-1782/e20240083pt
Silva FGD, Paula DD, Alves LM, Santos JN.To investigate evidence of horse riding in the development of language, cognition, social, emotional, and behavioral aspects in neurotypical children and adolescents. Methods: Search in the databases of LILACS, MEDLINE, Web of Science, EMBASE, Scopus, and grey literature, without date or language restrictions. Registration in the Open Science Framework (OSF), under number DOI 10.17605/OSF.IO/32ETZ. For search strategies: "Equine-Assisted Therapy", "Child or Adolescent Development", Cognition, Socialization, and "Child Behavior". Methods: Randomized clinical trials and non-randomized experiment...
Best practices for physiological data collection in youth with autism and co-occurring mental health diagnoses: Implications for human-animal intervention research.
MethodsX    March 27, 2025   Volume 14 103284 doi: 10.1016/j.mex.2025.103284
Smith CM, Weimann K, Widick M, Merritt T, Christensen H, Siegel M, Pan Z, Gabriels RL.The purpose of this paper is to serve as a catalyst for the human-animal interaction research field to improve scientific rigor and accelerate the knowledge of field-based physiological responses during equine-assisted services in youth with autism spectrum disorder. This paper outlines the best practices for collecting and analyzing electrocardiogram and electrodermal activity in youth with autism spectrum disorder, utilized during a 10-week therapeutic horseback riding intervention.•Motivation strategies such as device choice, reward systems, and a visual schedule should be implemented to ...
Prevalence and Risk Factors of Staphylococcus aureus Nasal Colonization in Horses Admitted to a Veterinary Teaching Hospital.
Journal of veterinary internal medicine    March 26, 2025   Volume 39, Issue 3 e70027 doi: 10.1111/jvim.70027
Allano M, Arsenault J, Archambault M, Fairbrother JH, Sauvé F.Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial infections, including in veterinary settings. Objective: To investigate the prevalence, risk factors for Staphylococcus aureus (SA) and MRSA colonization, and the duration of MRSA colonization. Methods: Elective cases admitted to the Veterinary Teaching Hospital were recruited (228 horses). Methods: A cross-sectional study was conducted over 3 years. Nasal swabs were collected at admission and cultured for SA. Methicillin-resistant isolates were identified using matrix-assisted laser desorption/ionization-time-...
The role of space availability and affiliation in shaping equine social distances and dynamics.
Scientific reports    March 25, 2025   Volume 15, Issue 1 10273 doi: 10.1038/s41598-025-92943-4
Torres Borda L, Auer U, Jenner F.Horses confined to enclosures with limited opportunities for spatial avoidance and escape experience heightened social stress and conflict. While forced proximity can pose welfare risks, voluntary social proximity is considered a positive indicator of well-being. To investigate the effects of space availability on spatiotemporal dynamics, social network structure and agonistic interactions, this study used ultra-wideband sensors to continuously (1 Hz) measure interindividual distances in 34 horses across three groups under paddock and field conditions. Interindividual distances increased in l...
Novel risk factors associated with fatal musculoskeletal injury in Thoroughbreds in North American racing (2009-2023).
Equine veterinary journal    March 25, 2025   doi: 10.1111/evj.14503
Bennet ED, Parkin TDH.The Equine Injury Database (EID) is a census-level record of Thoroughbred racing in North America, currently recording 95.6% of all race starts in 2023, along with partial training and veterinary histories of each horse. Objective: To identify horse-, race- and track-level risk factors associated with race-related fatal musculoskeletal injury (MSI) of Thoroughbred racehorses in North America. Methods: Retrospective cohort study. Methods: The study cohort included all race starts made by horses born after 31 December 2006, at tracks that fully report to the EID and consisted of 3,851,659 race s...
Equine colic outcomes and prognostic factors at a South African academic hospital (2019-2021).
Journal of the South African Veterinary Association    March 24, 2025   doi: 10.36303/JSAVA.672
van der Merwe LM, Schliewert EC.Colic is a common equine emergency requiring medical or surgical intervention. These interventions can be costly, making outcomes data and prognostic indicators essential for guiding referral and treatment decisions. Objective: To determine survival rates to hospital discharge and evaluate prognostic indicators in equine colic cases treated at a referral hospital (Onderstepoort Veterinary Academic Hospital [OVAH], South Africa). Methods: This retrospective observational cohort study analysed records from horses with colic that were presented to the OVAH from January 2019 to August 2021. Data i...
Risk factors for superficial digital flexor tendinopathy in Thoroughbred racehorses in South Korea (2015-2019).
Equine veterinary journal    March 19, 2025   doi: 10.1111/evj.14493
Choi Y, Parkin T.Superficial digital flexor (SDF) tendinopathy is one of the most common musculoskeletal diseases in Thoroughbreds and a major cause of retirement from racing among racehorses in South Korea. However, there are no previous epidemiological studies on SDF tendinopathy-related risk factors for racehorses in South Korea. The interventions derived from such a study could reduce the occurrence of SDF tendinopathy and, therefore, extend horses' racing careers. Objective: To identify the risk factors associated with SDF tendinopathy for Thoroughbreds in South Korea. Methods: Retrospective case-control ...
Tracking the Track: The Impact of Different Grazing Strategies on Managing Equine Obesity.
Animals : an open access journal from MDPI    March 19, 2025   Volume 15, Issue 6 doi: 10.3390/ani15060874
Cameron L, Challinor M, Armstrong S, Kennedy A, Hollister S, Fletcher K.Horses evolved to live in herds, continuously moving and foraging. Domestic horses often have limited social contact and movement, with high calorific intake, contributing to obesity. "Track grazing" is an increasingly popular management system, but evidence of its impact on weight management, behaviour, and welfare is limited. Horses (n = 19) resident at HorseWorld (UK) were observed July to October 2023, monitoring bodyweight (kg), Body Conditions Score (BCS), hoof health, behaviour, and welfare indicators. Horses on track grazing systems lost significantly more bodyweight (kg) (F(1,15) = 8....
Learning from eavesdropping on human-human encounters changes feeding location choice in horses (Equus Caballus).
Animal cognition    March 17, 2025   Volume 28, Issue 1 23 doi: 10.1007/s10071-025-01946-1
Krueger K, Roll A, Beyer AJ, Föll A, Bernau M, Farmer K.When animals observe human signals, they may learn from them. Such learning from humans has been reported for intentional communication between humans with animals, but animals might also learn socially by observing unintentional information transfer when eavesdropping on humans-human encounters. In this study, 12 of 17 horses significantly changed their preference for a feeding location after observing approval in a human-human interaction there, and horses kept in social housing adapted in a higher percentage of trials to human-human demonstrations than those in individual housing. This indi...
Evaluating Pelvic Floor Dysfunction in Female Horse Show Jumpers Using the Australian Pelvic Floor Questionnaire.
Medical science monitor : international medical journal of experimental and clinical research    March 16, 2025   Volume 31 e946830 doi: 10.12659/MSM.946830
Cygańska AK, Giżewska B, Zdunek K.BACKGROUND Pelvic floor dysfunction (PFD) significantly reduces quality of life. During horseback riding, the movement of the horse's back affects the tension of the pelvic floor muscles. This study aimed to compare reported symptoms of pelvic floor dysfunction in women with past, current, and no history of horse show jumping. MATERIAL AND METHODS We examined 160 women (age 23.69±3.96 years). The cohort group consisted of 74 women training currently and training in the past, and a control group of 86 women not practicing horseback riding. The study used the original questionnaire and the Aust...
Survey on 9 years of anti-doping controls in horse races in Italy.
Equine veterinary journal    March 13, 2025   doi: 10.1111/evj.14496
Roccaro M, Rinnovati R, Stucchi L, La Rocca F, Cascio G, Peli A.Doping in racehorses is a threat to horse welfare and the integrity of the sport. Despite its relevance, the literature on the prevalence of anti-doping violations worldwide is limited. Objective: To analyse anti-doping violations in racehorses in Italy. Methods: Retrospective observational study. Methods: Data on horse races, anti-doping testing, and confirmed violations between 2014 and 2022 were collected and analysed. Positivity rates, most common drug classes and parent drugs, and differences between trotting and galloping horses were investigated. Results: During the considered 9-year ti...
Effect of Massage on Stress Indicators in Recreational Horses-A Pilot Study.
Animals : an open access journal from MDPI    March 11, 2025   Volume 15, Issue 6 doi: 10.3390/ani15060789
Jastrzębska E, Górecka-Bruzda A, Ogłuszka M, Lipka MS, Pawłowska A.This pilot study investigated the effects of massage therapy on stress indicators in recreational horses, focusing on salivary cortisol levels, heart rate and conflict behaviors. Five recreational horses were analyzed under two experimental conditions: without and with massage treatments before riding sessions. Saliva samples were collected at four specific intervals in each condition to measure cortisol concentrations using an ELISA test. Observations of behavioral indicators and heart-rate monitoring complemented the physiological data. The results revealed that massage significantly reduced...
Enhancing equine welfare: a qualitative study on the impact of RAiSE (Recognizing Affective States in Equine) as an educational tool.
Translational animal science    March 11, 2025   Volume 9 txaf033 doi: 10.1093/tas/txaf033
Wells A, Hiney KM, Brady CM, Anderson KP.The horse industry's growing focus on improving horse welfare stems from stakeholders' desires, increasing public scrutiny, and potential threats to its Social License to Operate. Correctly assessing equine affective states is crucial for enhancing horse welfare. However, horse owners often struggle with this assessment, especially those with limited consistent access to horses. To address this gap, RAiSE (Recognizing Affective State in Equine) was developed as an educational tool aimed at improving horse industry participants' ability to recognize these states. After development of the educat...
Analysis of the Culturable Skin Microbiome of Horses from Southern Germany.
Microorganisms    March 8, 2025   Volume 13, Issue 3 623 doi: 10.3390/microorganisms13030623
Matinpour M, Zettner N, Neumann K, Bäumer L, Burkovski A.Horses have close interactions with humans and are important as working animals and livestock. In contrast to smaller companion animals like cats and dogs, there is only little information available about their skin microbiome. The objective of this study was to identify and characterize the culturable cutaneous microbiome of healthy horses. Samples were taken from 14 horses from Southern Germany which were randomly enrolled in this study. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used as a method to detect the culturable microorganisms of ...
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