Veterinary research in horses encompasses the study of diseases, health management, and medical treatments specific to equine species. This field investigates various aspects of horse health, including infectious diseases, metabolic disorders, and musculoskeletal conditions. Researchers focus on understanding the pathophysiology of equine ailments, developing diagnostic tools, and evaluating therapeutic interventions. The study of horse health also involves examining preventive measures such as vaccination protocols and nutritional management to promote overall well-being. This page collects peer-reviewed research studies and scholarly articles that explore the diverse areas of veterinary science related to horses, providing insights into disease mechanisms, treatment strategies, and advancements in equine healthcare.
Buchner HH, Savelberg HH, Schamhardt HC, Barneveld A.The kinematic pattern of mild bilateral lameness was studied by inducing a supporting limb lameness in both fore-limbs of 11 sound Dutch Warmblood horses. The kinematics of the horses were recorded while they trotted (3.5 m/s) on a treadmill. The locomotion analysis system CODA-3 was used to determine the temporal stride patterns, limb movements as well as head and trunk movement patterns. The transient lameness model, by which pressure-induced pain is evoked on the hoof sole, was used. Differences between left and right limbs as well as between the sound and the lame condition were tested usi...
Blissitt KJ, Bonagura JD.Thirty-two horses with cardiac murmurs typical of tricuspid, mitral and aortic regurgitation were studied using colour flow Doppler echocardiography. The dimensions and duration of any regurgitant signals, recorded at the cardiac valve suspected as being the site of origin of the murmur, were measured. Results were compared with flow signals described at the valves of normal horses (Blissitt and Bonagura 1995). Horses with murmurs suggestive of tricuspid (n = 8) and aortic (n = 8) regurgitation showed larger regurgitant signals at the tricuspid and aortic valves respectively, than has been rep...
Patteson MW, Gibbs C, Wotton PR, Cripps PJ.Twenty-six horses were sedated with detomidine hydrochloride (Domosedan, SmithKline Beecham Animal Health, Tadworth, Surrey, UK) at a dose of 10 micrograms/kg bwt, administered i.v. Echocardiograms were recorded before and after sedation, measurements of cardiac dimensions were made and functional indices were calculated. The pre- and post sedation values were compared. No significant change was detected following sedation in the ejection time (ET), left ventricular dimension, left atrial diameter at the sinotubular junction at end-systole or end-diastole or at the sinus of Valsalva at end-dia...
Patteson MW, Gibbs C, Wotton PR, Cripps PJ.Cardiac dimensions and indices of cardiac function were measured in 29 clinically normal adult Thoroughbreds (TB) which performed minimal exercise using guided M-mode and two-dimensional (2-D) echocardiography, from a variety of image planes and measurement methods, and standardised imaging planes based on intracardiac anatomical landmarks. The different methods of measuring these variables were compared. The variables which were considered to be most valuable for quantitative echocardiography were measured in a further 9 horses. Comparable measurements of cardiac dimensions can be obtained fr...
Reef VB.Physiological flow murmurs occur frequently in horses and may be difficult to distinguish from murmurs associated with underlying cardiac disease. The significance of heart murmurs auscultated in horses is often difficult to determine if the horse is not exhibiting any clinical signs or if the signs, such as poor performance, are nonspecific. A complete echocardiographic examination (M-mode, 2-dimensional (2-D) and Doppler) provides an objective assessment of the severity of the horse's underlying cardiac disease. Valvular regurgitation and ventricular septal defects (VSDs) occur frequently an...
Uboh CE, Rudy JA, Railing FA, Enright JM, Shoemaker JM, Kahler MC, Shellenberger JM, Kemecsei Z, Das DN.Although urine is the sample of choice for drug tests in racehorses, it is rarely obtained following the sudden death of a racehorse on the track while racing. The purpose of this study was to demonstrate the significance of postmortem tissue samples as an alternative to urine and blood samples in equine drug analysis following the sudden death of a racehorse on the track while participating in a competitive race. Postmortem tissue samples were frozen (-80 degrees C) until analyzed. A 30-40-g portion of each organ was homogenized in a 0.1 M phosphate buffer (pH 7.4), deproteinized, hydrolyzed ...
Marr CM, Reef VB.Colour flow Doppler echocardiographic examinations were performed on 15 horses; 10 Thoroughbreds and 5 Standardbreds; 9 mares, 5 geldings and 1 stallion; mean +/- s.d. 3.4 +/- 1.1 years. Cardiac murmurs were audible in 12 horses (80%), but these had characteristics consistent with functional murmurs in all horses. A standardised two-dimensional (2-D) and M-mode echocardiographic examination was unremarkable in all horses except that aortic valve prolapse was identified in 3 horses, tricuspid valve prolapse in 2 horses and mitral valve prolapse was present in one horse. Colour flow Doppler echo...
Reef VB.Ventricular septal defects (VSDs) were diagnosed in 27 horses; in 26 affected horses systolic murmurs were detected over both sides of the chest. Holodiastolic decrescendo murmurs were also detected in 5 horses. Standardbreds and Arabian horses were over-represented, while Thoroughbred horses were under-represented, when compared to the hospital population (P < 0.0001). Five horses had previously raced successfully, one 2-year-old was training successfully and close to racing, and 4 horses had competed successfully in other types of competition. Eleven horses had a history of exercise intolera...
Wallace FJ, Emery JD, Cripps AW, Husband AJ.The ability of mucosally administered antigen to provide protection against Streptococcus equi ('Strangles') infections in horses was examined. First, an enzyme linked immunosorbent assay (ELISA) was developed to detect the immune status of horses to S. equi. This assay was used to select Strangles-naive horses for the study and also to monitor their response to immunisation. Potential vaccine candidates were: (a) orally administered paraformaldehyde killed S. equi; (b) intraperitoneally (IP) administered paraformaldehyde killed S. equi in a non-inflammatory adjuvant; (c) orally administered l...
Awan AR, Baxi M, Field HJ.The most important consequence of equine herpesvirus-1 (EHV-1) infection is abortion. The object of the present study was to characteristic further a murine EHV-1 abortion model and to make comparisons with the natural host with particular reference to the stage of gestation during which the infection occurs. BALB/c mice at different stages of pregnancy were infected intranasally with EHV-1 (strain AB4); they suffered respiratory distress, weight loss, and other constitutional signs of infection. When the virus was inoculated in the late second or early third week of gestation dead or dying fe...
Brinsko SP, Ball BA, Ellington JE.Oocytes were harvested from mare ovaries obtained at slaughter and were divided into 3 groups based on the age of the donor. The age groups consisted of young (2 to 7 yr), middle-aged (8 to 14 yr) and aged (>or=15 yr) mares. There were no differences between age groups in the proportions of follicles available for examination or the proportions of normal, abnormal or total oocytes collected. After 24 h of culture, the overall maturation rate to the second metaphase (MII) was 52.7%. Maturation rates for oocytes obtained from young and middle-aged mares were similar, but oocytes from aged mar...
Pal M.A total of 257 samples (from 51 cattle, 43 buffalo, 32 goats, 25 dogs, 23 horses, 14 fowl, 9 camels, 7 rabbits, 5 donkeys, 4 antelopes, 3 pigs, 2 monkeys, 1 bear and 38 humans, all with cutaneous disorders) were examined for the presence of Dermatophilus congolensis using standard microbiological techniques. Dermatophilus was identified in 14 specimens (5.45%) both by direct microscopy and by cultural isolation of the pathogen from cutaneous specimens. The infection was recorded in 2 humans, 6 cattle, 3 buffalo, 1 goat, 1 horse and 1 antelope. A history of trauma to the skin was evident in 6 o...
Mason MW, Skedros JG, Bloebaum RD.The relative importance that certain strain features, including mode (e.g., tension vs. compression) and magnitude, have in affecting adaptive bone remodeling seen in normal skeletally mature bones remains controversial. The equine radius is used as a model because in vivo strain data show that the mid-to-proximal diaphysis receives a consistent history of predominantly cranial-caudal bending loads, in contrast to the distal diaphysis which receives relatively more torsional loading superimposed on cranial-caudal bending. Medial and lateral cortices serve as control regions because they corres...
Beech J, Lindborg S.Horses with hyperkalaemic periodic paralysis were challenged with an oral dose of potassium chloride, and the prophylactic efficacy of phenytoin, acetazolamide and hydrochlorothiazide was evaluated, with at least three weeks separating the trials of each drug. After the administration of potassium chloride without prophylactic medication the horses' clinical signs ranged from generalised fine muscle fasciculations to gross tremors, and weakness with occassional prolapse of the nictitating membrane; plasma potassium concentration increased significantly (P < 0.01) from 4.0 +/- 0.2 to 6.0 +/-...
Whalley JM, Love DN, Tewari D, Field HJ.A series of recombinant baculoviruses containing genes for glycoproteins C, D, H and L of equine herpesvirus 1 (EHV-1) have been constructed, and the EHV-1 products characterised by gel electrophoresis and immunoblotting. The EHV-1 glycoproteins expressed in insect cells were similar but not identical in apparent sizes to those expressed in EHV-1 infected mammalian cells. Each of the EHV-1 products was recognised by convalescent equine sera, indicating that they were all targets for an equine immune response. Mice immunised with baculovirus-expressed EHV-1 gD and gC acquired an enhanced abilit...
Crabb BS, Studdert MJ.A series of truncated equine herpesvirus 1 (EHV1) glycoprotein C (gC) molecules was examined for use as serodiagnostic antigens for EHV1 and EHV4. Small regions of EHV1 glycoprotein C, an immunodominant EHV1 glycoprotein, were expressed in Escherichia coli as glutathione S-transferase (GST) fusion proteins using the bacterial expression vector pGEX-2T. Sera obtained from horses, including sera from specific-pathogen-free (SPF) foals, following exposure to either EHV1, EHV4 or both viruses were used. Several of the fusion proteins were shown to encompass EHV1 specific epitopes while others enco...
Edwards RB, Ducharme NG, Fubini SL, Yeager AE, Kallfelz FA.The medical records of 34 horses with a diagnosis of avulsion of the origin of the suspensory ligament that had been admitted to the veterinary medical teaching hospital between 1980 and 1993 were identified. In addition to clinical examination, 21 of 34 horses had scintigraphy and radiography performed during their examination. The usefulness of scintigraphy and radiography were assessed by comparing the initial findings reported in the medical record to those obtained in a retrospective review of the images. Thirty other horses with scintigraphic lesions of the proximal aspect of the third m...
Slater MR, Hood DM, Carter GK.A descriptive and matched case-control study of laminitis was conducted in 7 private practices and at the Texas Veterinary Medical Centre (TVMC) between May 1992 and July 1993. Out of 108 horses with laminitis, 19 acute (49%) and 20 chronic (51%) cases were seen in private practice and 16 acute (23%) and 53 (77%) cases at the TVMC. Gastrointestinal disease was the most common problem in 19/35 horses (54%), occurring just prior to the onset of acute laminitis in all hospitals. Among all horses in the study, most commonly used drugs were phenylbutazone (68%), acepromazine (34%), dimethyl sulphox...
Grünig G, Triplett L, Canady LK, Allen WR, Antczak DF.Invading trophoblasts form endometrial cups in the endometrium of the pregnant mare. In the present study we characterized the maternal leucocyte response to endometrial cups from their formation to their regression. The maternal leucocyte response was correlated with the stages of trophoblast development. (1) Aggregates of CD4+ and CD8+ cells were present between the migrating and differentiating endometrial cup trophoblasts and surrounding the forming endometrial cups. (2) Numbers of CD4+ cells within the mature endometrial cups were much reduced. At the periphery of the endometrial cups CD4...
Fodor L, Szenci O, Peters M, Varga J, Szemerédi G, Wyszoczky F.A total of seven Bacteroides ureolyticus strains were isolated from the cervix and the clitoral fossa of mares with vaginal discharge. No other bacteria capable of causing metritis or vaginitis were isolated from the samples. The isolated strains resembled Taylorella equigenitalis. Both species are catalase, oxidase and alkaline phosphatase positive, but, in addition to these characteristics, B. ureolyticus strains produced urease and they could not tolerate 10% O2. They also failed to be agglutinated in a hyperimmune serum raised against T. equigenitalis; however, B. ureolyticus and T. equige...
Ko WH, Pediani JD, Bovell DL, Wilson SM.We have explored the properties of a Ca(2+)-dependent cell-signalling pathway that becomes active when cultured equine sweat gland cells are stimulated with ATP. The ATP-regulated, Ca(2+)-influx pathway allowed Sr2+ to enter the cytoplasm but permitted only a minimal influx of Ba2+. Experiments in which cells were repeatedly stimulated with ATP suggested that Sr2+, but not Ba2+, could become incorporated into the agonist-sensitive, cytoplasmic Ca2+ store. Further evidence for this was provided by experiments using ionomycin, a Ca2+ ionophore which has no affinity for Sr2+.
Dik KJ, Dyson SJ, Vail TB.The anatomy of the digital flexor tendon sheath and related tendons and ligaments is described. Diagnosis and management of acute tenosynovitis and long-term tenosynovitis and associated tendon injuries are discussed, as well as the syndrome of stenosis of the fetlock canal (or fetlock annular ligament constriction) and palmar annular ligament constriction. Desmitis of the palmar annular ligament is also described.
Rapp HJ, Jost H, Stechele M, Becker M.A report is given of obstructive ectasy of both kidneys in a newborn foal. Diagnosis and therapy are described as well as differential diagnosis.
Wright IM.This article contains a short introduction to the anatomy, physiology, and pathophysiology of ligaments associated with diarthrodial joints. Individual ligaments are discussed on a regional basis as forelimb, distal limb, and hindlimb. Emphasis is placed on the anatomy of the ligaments because this is the key to sound clinical application of their involvement in joint disease. There are few conditions of diarthrodial joints that do not involve their associated ligaments, and this role may be in causative, diagnostic, and therapeutic or convalescent considerations.
Nambo Y, Oikawa M, Yoshihara T, Kuwano A, Katayama Y.To investigate the morphological changes of uterine wall arteries associated with the progression of age in Thoroughbred broodmares, uterine wall arteries from 13 mares, aged 2 to 31 years, were studied by routine histomorphometry. The ratio of intimal thickness to external diameter increased due to hyperplasia of elastic fibers and was significantly correlated with age (r = 0.95, P < 0.001). The ratio of medial thickness to external diameter decreased and was also significantly correlated with age (r = -0.84, P < 0.001). Using electron microscopy, it was determined that the cross-sectional ar...
Clément F, Barrey E.Variability of heart rate was analysed within a sample of 30 horses in order to determine the influence of factors (animal, age, sex and breed) associated with different behavioural patterns. The heart rate fluctuations were analysed by computing the power spectral density of a series of heartbeat intervals. A generalized linear model was used to compare the spectra and to reveal the effect of each factor: animal age, sex and breed. The scale and shape of spectral densities were significantly different between animals. This individual variability was partly explained by the sex and age effects...
Henson FM, Davies ME, Skepper JN, Jeffcott LB.The aim of this study was to localise alkaline phosphatase (ALP) activity in equine growth cartilage both histochemically and ultrastructurally. For histochemical studies, full thickness growth cartilage samples were obtained from 6 anatomical sites from 16 horses and ponies ranging in age from 90 d postconception to 12 years of age. For ultrastructural studies, samples were obtained from the lateral trochlear ridge of the distal femur of 3 animals ranging in age from 157 d postconception to 12 months of age. Alkaline phosphatase in histological sections was localised using a substituted napht...
Schmidt A.Superficial mycoses caused by dermatophytes, as well as asymptomatic carriership of dermatophytes, have a high prevalence among domestic animals and pets. We examined 606 clinical specimens from skin lesions of animals with a significant tendency towards superficial mycosis due to their clinical features. Samples were obtained from horses, dogs, cats, small rodents, birds, and rabbits. The specimens were examined by microscopic and cultural techniques. Microscopically, there was no significant difference in the prevalence of structures which may develop fungal elements between the groups cultu...
Shaffer SK, Sachs N, Garcia TC, Fyhrie DP, Stover SM.To assess the motion of the proximal sesamoid bones (PSBs) relative to the third metacarpal bone (MC3) of equine forelimbs during physiologic midstance loads. Methods: 8 musculoskeletally normal forelimbs (7 right and 1 left) from 8 adult equine cadavers. Methods: Each forelimb was harvested at the mid-radius level and mounted in a material testing system so the hoof could be moved in a dorsal direction while the radius and MC3 remained vertical. The PSBs were instrumented with 2 linear variable differential transformers to record movement between the 2 bones. The limb was sequentially loaded ...
Recio P, López PG, Fernández JL, Garcia-Sacristán A.1. The presence and types of alpha and beta-adrenoceptors in the corpus cavernosum of the horse were studied in vitro by using selected ligands of adrenoceptors and isometric tension recording. 2. Noradrenaline and phenylephrine induced concentration-dependent contractions in corpus cavernosum preparations. B-HT 920 had no effect. 3. Phentolamine and prazosin produced a shift to the right of the dose-response curve of noradrenaline, while the alpha(2)-antagonist, rauwolscine had no effect on the response to noradrenaline. Phenylephrine-evoked contractions of corporal strips were significantly ...
Bishop RC, Wilkins PA, Kemper AM, Stewart RM, McCoy AM.Nonsteroidal anti-inflammatory drug (NSAID) administration carries risks of gastrointestinal toxicity. Selective COX-2 inhibitors ("coxibs") were designed to reduce risks of adverse effects but are still associated with gastrointestinal complications in humans. The effect of coxibs on colonic inflammation and integrity in horses is unknown. The study objective was to compare the effects of the coxib firocoxib and the nonselective NSAID flunixin meglumine on ultrasonographic indicators of colonic inflammation in healthy horses. Twelve healthy adult horses were administered flunixin meglumine (1...
Black JA, Leaf G.1. The effects of cyanogen bromide on horse-heart cytochrome c and horse-heart myoglobin have been investigated. Cytochrome c yielded four fragments, of which two were haemopeptides. The two colourless peptides had amino acid compositions corresponding to those that are expected, on the basis of the sequence proposed for horse-heart cytochrome c by Margoliash, Smith, Kreil & Tuppy (1961), from cleavage at both methionine residues. Of the two haemopeptides, one was isolated and shown to be that derived from cleavage at only one methionine residue, that nearer to the C-terminus of the peptid...
Nagy AM, Sathe SR, Atta AH, Hammam AMM, Hsu WH.In equine parturition, the role of progestins along with the nuclear progesterone receptor (nPR) signaling pathway in the placenta is not completely clarified. The progestins play an integral role in maintaining myometrial quiescence during the late stage of pregnancy via acting on nPR isoforms (PRA and PRB; PRB is more active than PRA). The current study aimed to determine the PRA and PRB expressions in the term equine placenta at the gene and protein levels. Six term equine placentas were used in this study. Reverse transcription polymerase chain reaction (RT-PCR) was used to quantify the mR...
Clayton HM, St George L, Sinclair J, Hobbs SJ.Show jumping horses must execute fences of varying height and width, but the effect of this on jumping kinematics during the airborne phase have not been described. The aim of this study was to describe differences within- and between-horses in CM trajectory, trunk orientation and average trunk angular velocity in a group of elite horses executing three fences: vertical fence (1.60 m), spread fence (1.50 × 1.80 m), water jump (4.5 m) during an Olympic competition. Two-dimensional kinematic data (60 Hz) were collected from video cameras set perpendicular to each fence. After manual digitizat...
Watson ED, Thomson SR, Howie AF.A steroidogenic acute regulatory (StAR) protein has been identified in several species as a probable important rate-limiting step in steroidogenesis. This protein is believed to be responsible for transporting cholesterol from the outer to the inner mitochondrial membrane. It is known that equine chorionic gonadotrophin (eCG) stimulates steroidogenesis in the corpora lutea of early pregnant mares and that eCG also upregulates StAR mRNA in bovine ovaries. In the present study, ovarian tissue from cyclic and early pregnant mares was immunostained to detect the distribution of the StAR protein. W...
Possessing high meat and dairy productivity and good reproductive and adaptive qualities to year-round grazing, the Kushum breed plays an exceptional role in the improvement of productive qualities in horse herding in Kazakhstan and the former Soviet republics. The aim of the study was to develop breeding methods for creating new highly productive breeding lines for the Kushum breed horses. The leading method was linebreeding, which is based on the systematic use of remarkable animals, the offspring of which will accumulate and develop all the desired qualities and traits. The practical signif...
Kanitz W, Schneider F, Hoppen HO, Unger C, Nürnberg G, Becker F.The aim of the study was to investigate the effect of the GnRH agonist Buserelin given on day 10 after ovulation on pregnancy rate and concentrations of progesterone and LH. Altogether 191 warmblood mares were used for two trials. Fresh or frozen/thawed semen from 27 stallions was used for A.I. In trial A 171 mares received either Buserelin (Receptal, Hoechst, Germany, 40 microg/animal) or 10 ml 0.9% NaCl (placebo). On day 16 after A.I. pregnancy diagnosis was performed by ultrasound scanning of the uterus. For statistical analysis, data were analyzed by a mixed model, with four fixed factors ...
de Waal A, Potgieter FJ.Dehydration, to varying degrees, will inevitably occur in horses participating in endurance trail rides. This water loss is directly related to the amount of body water lost through evaporative cooling, that in turn being related to the amount and rate of work performed, and to the environmental temperature and humidity. An electronic apparatus that can substitute for the conventional and time-consuming venous haematocrit was developed to measure the animals' hydration status. The mechanism of this electrometer is based upon the relationship between the red cell content and the electrical cond...
Svalastoga E, Nielsen K.The histopathological changes in the synovial membrane of bursa podotrochlearis are described in horses with navicular disease. The changes are compared to the histopathological findings in synovial membrane of arthrotic pastern joints in horses and of arthrotic hips in man. A high degree of concordance is demonstrated and this suggests that navicular disease is an arthrotic disease.
Todhunter RJ, Fubini SL, Vernier-Singer M, Wootton JA, Lust G, Freeman KP, MacLeod JN.To determine how acute synovitis, with and without intra-articular methylprednisolone acetate (MPA), affect synthesis of proteoglycan, total protein, and collagen in articular cartilage and total protein synthesis in synovial membrane. Methods: Synovitis was induced in 10 ponies by the injection of 0.5 ng lipopolysaccharide (LPS) into the left radiocarpal and midcarpal joints every 2 days for a total of four treatments. Synovitis was documented by clinical examination and synovial fluid analyses. Two days before euthanasia, MPA (0.1 mg/kg) was injected with the last dose of LPS into both the l...
DiPietro JA, Lock TF, Todd KS, Sanecki RK.A controlled test was carried out on 15 pony foals inoculated with 1,500 +/- 108.8 infective Parascaris equorum eggs. The foals were assigned to 3 treatment groups. Treatments given on postinoculation day 11 included 0.2 mg of ivermectin/kg of body weight, formulated as paste (n = 5), or liquid (n = 5), or no treatment (controls; n = 5). The foals were euthanatized on postinoculation day 25, and examined for larvae in the small intestine, lungs, and liver. Larvae were not found in foals treated with ivermectin liquid or paste, whereas significantly (P less than 0.05) higher mean numbers (960.9...
Zarghooni K, Hub L, Bredow J, Yagdiran A, Hackenberg RK, Scheyerer M, Westermann L, Eysel P, Siewe J.Equestrian vaulting is a sport, particularly popular among children and adolescents, in which gymnastic and dance routines are performed on horseback. Current data regarding injuries and thus, the risks of this sport, is meager and based only on retrospective studies. Methods: In the current prospective study, 233 active members of a vaulting club were questioned monthly from November 2014 until October 2015. In addition to general information (training, competitions), the questionnaire collected the number of competitions, the competitive class, the discipline (single, team, Pas-de-Deux), and...
Lepeule J, Seegers H, Rondeau V, Robert C, Denoix JM, Bareille N.Developmental Orthopaedic Disease (DOD) in limb joints is frequent, can cause lameness and is a major cause of economic losses for the horse breeding industry. Studies on risk factors for DOD usually dichotomise the outcome to presence/absence without considering the extent of the disease, which can be appreciated by the number or the severity of the lesions. The aim of this study was to assess the effects of growth, exercise conditions and feeding practices on the number of radiographic findings (RF) of DOD in a cohort of horses, assuming that the risk factors associated with the presence of ...
Zoppa AL, Santoni B, Puttlitz CM, Cochran K, Hendrickson DA.To compare the biomechanical characteristics of 2 arthrodesis techniques for the equine proximal interphalangeal joint (PIP) using either a 3-hole 4.5 mm locking compression plate (LCP) or 3-hole 4.5 mm narrow dynamic compression plate (DCP), both with 2 transarticular 5.5 mm cortex screws. Methods: Experimental. Methods: Cadaveric adult equine forelimbs (*n=6 pairs). Methods: For each forelimb pair, 1 limb was randomly assigned to 1 of 2 treatment groups and the contralateral limb by default to the other treatment group. Construct stiffness, gap formation across the PIP joint, and rotation ab...
Wijnberg ID, van Dam KG, Graaf-Roelfsema Ed, Keizer HA, van Ginneken MM, Barneveld A, Breda Ev, van der Kolk JH.Too intensive training may lead to overreaching or overtraining. To study whether quantitative needle electromyography (QEMG) is more sensitive to detect training (mal)adaptation than muscle enzyme activities, 12 standardbred geldings trained for 32 wk in age-, breed-, and sex-matched fixed pairs. After a habituation and normal training (NT) phase (phases 1 and 2, 4 and 18 wk, respectively), with increasing intensity and duration and frequency of training sessions, an intensified training (IT) group (phase 3, 6 wk) and a control group (which continued training as in the last week of phase 2) w...
McLean R, Rash NL, Robinson C, Waller AS, Paillot R.Streptococcus equi subspecies equi (S. equi) is the causative agent of strangles, a highly contagious upper respiratory disease of equids. Streptococcus equi produces superantigens (sAgs), which are thought to contribute to strangles pathogenicity through non-specific T-cell activation and pro-inflammatory response. Streptococcus equi infection induces abscesses in the lymph nodes of the head and neck. In some individuals, some abscess material remains into the guttural pouch and inspissates over time to form chondroids which can harbour live S. equi. The aim of this study was to determine...
Lapo RA, Gogny M, Chatagnon G, Lalanne V, Harfoush K, Assane M, Desfontis JC, Mallem MY.This work was designed to investigate (i) the effect of superoxide dismutase (SOD) inhibition on endothelial function and (ii) the free radical-induced endothelial dysfunction in equine digital veins (EDVs) and equine digital arteries (EDAs) isolated from healthy horses. EDV and EDA rings were suspended in a 5 ml organ bath containing Krebs solution. After a 60 min equilibration period, EDV and EDA rings were contracted with phenylephrine. Then, cumulative concentration-response curves (CCRCs) to acetylcholine were performed. In both EDVs and EDAs, acetylcholine (1 nM to 10 µM) produced conce...
Lubow BC, Ransom JI.Reliably estimating wildlife abundance is fundamental to effective management. Aerial surveys are one of the only spatially robust tools for estimating large mammal populations, but statistical sampling methods are required to address detection biases that affect accuracy and precision of the estimates. Although various methods for correcting aerial survey bias are employed on large mammal species around the world, these have rarely been rigorously validated. Several populations of feral horses (Equus caballus) in the western United States have been intensively studied, resulting in identifica...
Larrasa J, Garcia A, Ambrose NC, Alonso JM, Parra A, de Mendoza MH, Salazar J, Rey J, de Mendoza JH.Dermatophilus congolensis is the pathogenic actinomycete that causes dermatophilosis in cattle, lumpy wool in sheep and rain scald in horses. Phenotypic variation between isolates has previously been described, but its genetic basis, extent and importance have not been investigated. Standard DNA extraction methods are not always successful for D. congolensis due to its complex life cycle, one stage of which is encapsulated. Here we describe the development of rapid and reliable DNA extraction and random amplified polymorphic DNA (RAPD) methods that can be used for genotyping D. congolensis fie...
Mayaki AM, Abdul Razak IS, Mohd Adzahan N, Mazlan M, Abdullah R.Equine chronic back pain (CBP) has been linked to different pathologic processes, which directly or indirectly involve spinal structures. Thus, making diagnosis and management very challenging with most horses with the condition recommended for early retirement from athletic activity. This study described the spinal cord lesions and the development of reactive microgliosis and astrocytosis in the spinal cords of horse with CBP. Thoracolumbar spinal cord segments from three horses euthanized because of unresolved CBP were dissected and grossly and histopathologically examined. The expression of...
Fitzgerald AH, Magnin G, Pace E, Bischoff K, Pinn-Woodcock T, Vin R, Myhre M, Comstock E, Ensley S, Coetzee JF.Marijuana toxicosis is typically seen by companion animal veterinarians. However, with increased marijuana availability, there is a greater potential for toxicosis in other species. Herein we describe a case of suspected marijuana toxicosis in a female and a male American Mammoth donkey, aged 8 y and 20 y, respectively, fed cannabis buds. Both cases were presented because of depression and lethargy. However, the jenny had ataxia, mild colic, tachycardia, tachypnea, and decreased tongue tone. Plasma samples from the jenny on presentation and 3 d following hospitalization were submitted to...
Alef M, Oechtering G.Capnography measures carbon dioxide concentration or partial pressure of the respiratory gas continuously and non-invasively. The endtidal value is of great diagnostic value, it corresponds approximately to the arterial value, except for ventilation-perfusion-mismatching (horse), shunting (horse), or increased dead-space-ventilation (panting in the dog). Capnography primarily serves for monitoring of spontaneous and artificial ventilation, it is a reliable method for detecting hypo- and hyperventilation. Because metabolism and circulation influence the amount of carbon dioxide eliminated in th...
Ghosh S, Kjöllerström J, Metcalfe L, Reed S, Juras R, Raudsepp T.We present cytogenetic and genotyping analysis of a Thoroughbred foal with congenital neurologic disorders and its phenotypically normal dam. We show that the foal has non-mosaic trisomy for chromosome 26 (ECA26) but normal 2n = 64 diploid number because two copies of ECA26 form a metacentric derivative chromosome der(26q;26q). The dam has normal 64,XX karyotype indicating that der(26q;26q) in the foal originates from errors in parental meiosis or post-fertilization events. Genotyping ECA26 microsatellites in the foal and its dam suggests that trisomy ECA26 is likely of maternal origin and tha...
Ohmura H, Hiraga A, Aida H, Takahashi T, Nukada T.To determine oral dosage and to evaluate the pharmacokinetics in horses of orally administered flecainide, an antiarrhythmic drug, the correlations between its plasma concentration and PR, QRS and QT intervals in equine electrocardiograms (ECG) were investigated. Six healthy horses were administered a randomly ordered dose of 4 or 6 mg/kg of flecainide acetate. The ECG was monitored (heart rate (HR), PR, QRS, and QT intervals) and blood was taken at timed intervals to measure the plasma flecainide concentrations pre- and post-administration. The maximum plasma concentration reached 1014+/-285 ...
Raisis AL.The objective of this review was to describe the methodology and limitations of techniques that have been used to measure skeletal muscle blood flow in anaesthetized horses. Methods: Pubmed, personal files. Conclusions: Numerous techniques have been used in horses to study skeletal muscle blood flow during anaesthesia and after the administration of vasoactive agents. Of the available techniques, blood flow measurements are limited to either microvascular flow (radioactive xenon, laser Doppler flowmetry) or total blood flow (radioactive microspheres, electromagnetic flowmetry, Doppler ultrason...
Delbeke FT, Debackere M.The prototype of a commercial ELISA test kit designed for fentanyl determination in human urine has been evaluated for screening fentanyl in horse urine and plasma. The measurement of fentanyl after intravenous (2 mg) and intramuscular (0.25 mg) administration in undiluted plasma was not reproducible while accurate quantification of fentanyl in urine greatly depends on the composition of the horse urine. The ELISA assay, however, is simple and could be successfully used for quantitative measurements in diluted urine and for rapid qualitative screening for fentanyl in large numbers of urine sam...
Yilma T, Perryman LE, McGuire TC.The results of a study on the induction of IFN-alpha, IFN-beta, and IFN-gamma in normal and SCID foals showed a deficiency of IFN-gamma but not IFN-beta in SCID foals. The ability of SCID mononuclear cells to produce IFN-alpha in response to poly I:C but not to NDV may indicate a partial deficiency of IFN-alpha in SCID foals. The deficiency of IFN-gamma and presence of IFN-beta in SCID foals supports the classification of IFN-gamma and IFN-beta as immune and nonimmune interferons, respectively. Furthermore, the deficiency of IFN-gamma in SCID foals may in part explain the high susceptibility t...
