Veterinary research in horses encompasses the study of diseases, health management, and medical treatments specific to equine species. This field investigates various aspects of horse health, including infectious diseases, metabolic disorders, and musculoskeletal conditions. Researchers focus on understanding the pathophysiology of equine ailments, developing diagnostic tools, and evaluating therapeutic interventions. The study of horse health also involves examining preventive measures such as vaccination protocols and nutritional management to promote overall well-being. This page collects peer-reviewed research studies and scholarly articles that explore the diverse areas of veterinary science related to horses, providing insights into disease mechanisms, treatment strategies, and advancements in equine healthcare.
Ibrahim L, Vernemmen I, Buschmann E, van Loon G, Cornillie P.The interatrial septum morphology, shaped by the septum primum and secundum fusion, results in the formation of the fossa ovalis (FO) and its limbus. Incomplete fusion can lead to a patent foramen ovale (PFO), while complete fusion may produce septal ridges and pouches (SPs), with SPs in humans linked to ischemic stroke and atrial arrhythmias. In horses, atrial tachycardia and fibrillation often originate near the FO. This study examines adult equine interatrial septum morphology to enhance understanding the region and guide electrophysiological interventions for equine cardiac arrhythmias. Po...
Yuen BP, Wong KS, So YM, Kwok WH, Cheung HW, Wan TSM, Ho EN, Wong WT.The development of gene therapy techniques introduces a potential risk of gene doping, which threatens the integrity of sport. In response to this challenge, we have developed a novel analytical method that employs a multiplex polymerase chain reaction (PCR) in conjunction with liquid chromatography high-resolution tandem mass spectrometry (LC-HRMS/MS) for the simultaneous identification of multiple transgenes in equine plasma within a single reaction. The method targets three potential doping transgenes: equine growth hormone 1 (eGH1), equine growth hormone-releasing hormone (eGHRH), and equi...
Bhoora RV, Mbaba TV, Troskie M, Ackermann RE, Collins NE.Theileria haneyi is an apicomplexan parasite closely related to Theileria equi, a known causative agent of equine piroplasmosis. The molecular distinction between these parasites relies on a nested polymerase chain reaction (PCR) assay, which has been reported to be unreliable. A recently reported indirect ELISA based on equi merozoite antigen 11 (Thema-11) of T. haneyi can detect geographically diverse T. haneyi strains. Since the ema-11 gene is exclusive to T. haneyi, it was chosen as the target for developing a TaqMan minor groove binder (MGB™) quantitative real-time PCR (qPCR). Published...
Hannan MA, Watanabe H, Takeyama A, Yoshida S, Wudamu D, Lkhagvasuren N, Claes A, Stout TAE, Cheong SH, Haneda S, Nambo Y.This study evaluated the viability of in vitro embryo production using ovum pick-up (OPU) and intracytoplasmic sperm injection (ICSI) as breeding techniques for pure and crossbred Hokkaido native ponies (n = 9). Oocytes were collected using transvaginal ultrasound-guided follicle aspiration. ICSI was performed on in vitro matured oocytes using frozen semen. Embryonic cultures were monitored using time-lapse cinematography. Blastocysts were cryopreserved and, after thawing, were transferred non-surgically into recipient mares. Over nine OPU sessions, the mean number of aspirated follicles was 2...
The potential of effective microorganisms in composting animal manure might be significant. This work aimed at isolating promising microbial strains for composting cattle and horse manure. A total of forty-five isolates have been isolated from soils of Northern Kazakhstan as well as cattle and horse manure. These microorganisms were extensively studied for their cellulose-degrading capacity, non-pathogenicity, protease, nitrogenase and catalase activities, as well as their growth stimulation, antagonistic ability, and growth rate. As a result, 21 potent strains were selected and genotyped for ...
Furtado T, Perkins E, Archer DC.Research on the outcomes of veterinary treatments in dogs, cats and horses has important benefits for these animals and their owners. However, this information is not always available, and the evidence base is often lower-quality than in human medicine. To identify ways to improve the generation of evidence, we investigated the views of veterinary professionals about research involving companion animal patients and their owners. This qualitative study used semi-structured interviews with veterinary surgeons and registered veterinary nurses working in UK companion animal clinical practice. Inte...
Kadic LIM, Liu CC, Riggs LM.The aim of this study was to compare the monotonic tensile strength and fatigue strength of a stainless steel cable (SSC) compared to a human non-metallic polymer cable (NMPC) in an metacarpophalangeal joint arthrodesis model lacking a dorsal plate application.An experimental biomechanical equine cadaver limb study. Ten metacarpophalangeal joints were collected from five adult Thoroughbred horses, euthanatized for reasons unrelated to orthopaedic disease. Each pair of metacarpophalangeal joints was randomly instrumented with either a 1.7-mm SSC or a NMPC. Each construct was tested in cyclic ...
Avison A, Physick-Sheard PW, Pyle WG.This paper reviews the myocardial substrate of horses relative to that of humans and discusses the utility of performance horses as a model of exercise-associated cardiac arrhythmias and sudden cardiac death in athletes. The coronary circulation is similar between the species while coronary artery anomalies and myocardial bridging appear to only be associated with athletic mortality in human athletes and not in performance horses. There are subtle differences in the histology of the sinus and atrioventricular nodes, of unknown clinical significance, while the His bundle is more highly innervat...
Secor EJ, Womack SJ, Ysebaert MP, Colville MJ, Reesink HL.Synovial fluid (SF) is an ideal sentinel fluid for osteoarthritis (OA) diagnosis and prognostication due to its critical homeostatic role, proximity to articular tissues and immune cell composition. Untargeted proteomics enable identification of soluble markers for diagnostic and therapeutic applications while minimising bias. Objective: To use liquid chromatography-tandem mass spectrometry (LC-MS/MS) to define the SF proteome in horses with and without carpal OA. The goal was to identify differentially regulated proteins in mild-moderate carpal joint disease compared with healthy joints. Meth...
Hosseinzadeh S, Nofouzi K, Hasanzadeh F, Esmaeili S, Ayen E.Q fever is a frequently occurring illness that is induced by the bacterium ) that can infect humans and various animals. It targets the macrophage cells in the tissues, and circulating monocytes. Unassigned: This study was conducted between 2022 and 2023 in the West Azerbaijan and Ardabil provinces of northwestern Iran to examine the presence infection of . Specimens were obtained by swabbing from 140 mares (70 from each province) and 20 stallions (10 from each province) which were apparently healthy, and their DNA was analyzed using quantitative PCR assay detecting the element of the bacteri...
Hosseinzadeh S, Nofouzi K, Hasanzadeh F, Esmaeili S, Ayen E.Q fever is a frequently occurring illness that is induced by the bacterium ) that can infect humans and various animals. It targets the macrophage cells in the tissues, and circulating monocytes. Unassigned: This study was conducted between 2022 and 2023 in the West Azerbaijan and Ardabil provinces of northwestern Iran to examine the presence infection of . Specimens were obtained by swabbing from 140 mares (70 from each province) and 20 stallions (10 from each province) which were apparently healthy, and their DNA was analyzed using quantitative PCR assay detecting the element of the bacteri...
Berner D, Hellige M, Harvey GG, Gerlach K, Iglesias-Garcia M, Manso-Diaz G.Paranasal sinus cysts (PSC) and progressive ethmoid haematomas (PEH) are regularly observed in the paranasal sinus system. Due to their different prognosis and treatment options, differentiation of both lesions prior to surgery on computed tomographic (CT) images is important for guidance. It was hypothesised that PSC and PEH can be reliably differentiated on CT images. The database of four European equine referral centres were retrospectively searched for horses that had undergone a CT examination of the head and had a confirmed PSC or PEH. Additionally, cases with confirmed sinusitis were se...
Cito F, Di Francesco CE, Averaimo D, Chiaverini A, Alessiani A, Di Domenico M, Cresci M, Rulli M, Cantelmi MC, Di Bernardo MD, Giammarino A.... subsp. (SEZ) is a major problem in equine veterinary medicine. Typically, a commensal in horses, SEZ can cause severe disease including respiratory infections, septicaemia and reproductive tract infections under certain conditions. Recent evidence suggests that humans can also develop severe disease infection through direct contact with infected animals or the consumption of contaminated unpasteurised milk and milk products. This study investigates SEZ strains isolated from nasal swabs of equidae in central Italy in 2023 to describe the epidemiology and genomic characteristics of circulating...
Domino M, Borowska M, Stefanik E, Domańska-Kruppa N, Skibniewski M, Turek B.In equine surface electromyography (sEMG), challenges related to the reliability and interpretability of data arise, among other factors, from methodological differences, including signal processing and analysis. The aim of this study is to demonstrate the filtering-induced changes in basic signal features in relation to the balance between signal loss and noise attenuation. Raw sEMG signals were collected from the quadriceps muscle of six horses during walk, trot, and canter and then filtered using eight filtering methods with varying cut-off frequencies (low-pass at 10 Hz, high-pass at 20 Hz...
Filho RRR, França DS, Alonso MA, Riccio AV, Affonso FJ, Brito MM, Francischini MCP, Nichi M, Boakari Y, Fernandes CB.During gestation, there is a higher energy demand which increases oxygen requirements. As a result, there is an elevated production of reactive oxygen species. Notably, unlike in horses, the effects of maternal age and parity on women's oxidative profiles are well-documented. Thus, this study aimed to investigate oxidative changes in mares during the last third of gestation and the postpartum period, considering age and parity. Seventeen mares were categorized by age (3-7, 8-11, ≥12 years) and parity (nulliparous, 1-3, 4-6 parities). Oxidative processes were assessed using Thiobarbituric Aci...
Warren AS, Werner LA, Thombs LA, Rodgerson DH, Spirito MA, Sandow CB, McCracken MJ.To report the impact of ventral midline colopexy on colic, recurrence of large colon volvulus (LCV) and time to next live foal. Methods: Retrospective case control study. Methods: A total of 188 Thoroughbred broodmares surgically treated for LCV (2016-2020). Methods: Horses treated for LCV were grouped based on whether they received a colopexy (CP) or did not (NCP). All broodmares that underwent a second LCV surgery were subsequently categorized into two groups: those that received a colopexy (RCP) and those that did not (NRCP). Differences in the number of horses with large colon compromise, ...
Szacawa E, Kozieł N, Brzezińska S, Augustynowicz-Kopeć E, Weiner M, Szulowski K, Krajewska-Wędzina M. is one of the most dangerous pathogens of both animals and humans. Bovine tuberculosis (BTB) is a disease caused by mycobacteria belonging to the complex (MTBC), which spreads mainly among domestic cattle but also to mammals other than cattle. The transmission of MTBC between different species requires research and epidemiological investigations to control its spread. When multiple species are a reservoir of infection, it poses a significant public health and veterinary concern. In this study, the diagnosis of alpaca, cattle, horses, dogs, a sheep and a cat from one farm suspected of bovine ...
Loftus L, Asher L, Leach M.Over the last twenty years the definition of good animal welfare has advanced from the 'absence of negative welfare states' to the aim of identifying the presence of positive welfare states; however, research on positive animal welfare is relatively new. Consequently, through expert consultation, this study aimed to synthesise knowledge regarding domesticated equine emotional state, specifically methods to induce and measure positive affective states, which could be of significant benefit to equine welfare across sectors. A Delphi consultation of experts in the field of equine behaviour, welfa...
Fernandes T, Uberti B, Robin M.Utrasonographic measurements of the abdominal viscera and the heart in growing Arabian foals have not been described. Objective: To establish normal intervals for ultrasonographic measurements of cardiac indices and abdominal viscera in growing Arabian foals from birth to 1 year of age. Methods: Prospective longitudinal descriptive study. Methods: Ultrasonographic images were captured from 15 foals every 3 months from 1 week to 1 year of age. During each examination, at least three images were obtained at each location and measurements were obtained using standardised ultrasonographic approa...
Cardinaux EM, Oltmanns H, Beineke A, Meißner J, Geburek F.Flexural limb deformities are a widespread condition in foals. Oxytetracycline is a common conservative treatment option with relaxing effects on the muscle-tendon-unit, potentially mediated through a matrix-metalloproteinase (MMP)-inhibitor mechanism. Its high therapeutic dose for this indication, potential negative side effects, and the guidelines for prudent use of antimicrobials make investigating alternatives desirable. In this study, the influence of substances with potentially similar mechanisms of action, however without antimicrobial properties, on viability and proliferation of juven...
Connysson M, Jansson A.It is generally accepted that plasma muscle enzyme activity of creatine kinase (CK) and aspartate aminotransferase (AST) may increase in racehorses after exercise and racing, indicating muscle fibre damage and/or increased leakage from muscle fibres. However, other studies suggest that starch intake might influence plasma muscle enzyme activity reported postexercise. This study aimed to evaluate the effect of different starch allowances on plasma muscle enzyme activity in Standardbred trotters in professional training. Seventy-six horses from five professional trainers were sampled pre- and po...
Reinthaler B, Gabner S, Apprich V, Jehle M, Licka T.Equine hoof canker is a chronic proliferative condition of the hoof tissues that is often time-consuming and frustrating to treat. After surgical debridement, topical application of cisplatin (cis-Diamindichlorplatin II) has been reported, requiring repeatedly handling this hazardous substance during bandage changes. Alternatively, intraarterial application of cisplatin could be used, similar to the treatment of some human neoplastic diseases. As the side effects of such a treatment are currently unknown, evaluation of associated risks is necessary before treating live horses. Thus, forelimbs ...
Ostmeier M, Schellenberger F, Troillet A, Scharner D.Mouth specula with incisor bite plates shift the pressure from all teeth exclusively to the incisors in the opening phase which leads to increased forces on dental, osseous, and soft tissue structures of the horse's head. The potentially pathological character of these forces is described clinically by single reports of maxilla and mandible fractures occurring during the use of opened mouth specula. This study describes these forces on incisor teeth in horses during odontoplasty of cheek teeth under sedation. Measurements were documented using a modified "Günther" mouth speculum fitted with a...
Ciamillo SA, Bills KW, Gassert TM, Richardson DW, Brown KA, Stefanovski D, Ortved KF.Stress-induced bone injury can lead to catastrophic injuries in Thoroughbred racehorses. Accumulation of high-speed exercise can increase the risk of subchondral bone injury. Objective: To investigate the effect of high-speed exercise on the subchondral bone of the metacarpo-metatarsophalangeal (MCP/MTP) joints using standing cone-beam computed tomography (CBCT) in young racing Thoroughbreds. Methods: Observational cohort study. Methods: Forty-one 2-year-old Thoroughbred racehorses during their first year of training were evaluated at 0, 6 and 12 months. Horses were evaluated for lameness an...
Cantatore F, Marcatili M, Giacchi A, Stephenson R, Withers J.Morel-Lavallée lesions (MLLs) are post-traumatic, closed, degloving injuries observed in humans and cats. Morel-Lavallée lesions have never been reported in horses. An 8-year-old Warmblood gelding used for dressage presented with a large swelling on the craniomedial aspect of the stifle that did not respond to conservative treatment. Ultrasonography revealed a large anechogenic fluid-filled cavity with a well-defined capsule. Standing endoscopic examination revealed separation between the deep crural fascia, vastus medialis muscle and skin. Cytology of the fluid collected demonstrated a lymp...
Milczek-Haduch D, Żmigrodzka M, Witkowska-Piłaszewicz O.Significant systemic metabolic benefits result from even a single exercise session by activating multiple metabolic and signaling pathways within the organism. Among these mechanisms, extracellular vesicles (EVs) play a critical role by delivering their molecular cargo to neighboring or distant cells, thereby influencing cellular metabolism and function. As research progresses, EVs represent an exciting frontier in exercise science and fitness adaptation processes. There is increasing interest in understanding the physiology of EVs as signaling particles and their use as minimally invasive dia...
Bouquet A, Nicol C, Knowles EJ, Schofield I, Menzies-Gow NJ.Clinical signs of pituitary pars intermedia dysfunction (PPID) are frequently mistaken for 'normal' ageing and may not be optimally assessed. Objective quality of life (QoL) assessment could improve clinical decision-making. Objective: To develop an owner-reported health-related quality-of-life (HRQoL) assessment tool for equines with PPID. To assess factors associated with HRQoL scores. Methods: Quantitative, cross-sectional study. Methods: HRQoL tool development followed a standard psychometric process of item (any aspect of PPID and its management that could impact QoL) identification (foll...
Pádua GT, Tavares MA, de Lima NJ, Paula WVF, Dos Santos GC, Neves LC, Bittencourt RBM, Paludo RLDR, Cardoso ERN, da Silva BBF, Pádua BR....This study sought to investigate the presence of anti- spp. antibodies in georeferenced serum samples from equids across all regions of the state of Goiás, while also presenting variables that indicate risk factors for the circulation of rickettsiae, and evaluating the presence of rickettsial DNA in ticks collected from equids and the surrounding environment in the municipalities of Uruaçu and Porangatu, located in the northern region of the state. A total of 1156 equid serum samples provided by the Goiás Agricultural Defense Agency (Agrodefesa) were analyzed for antibodies against 4 antig...
Tagliavia C, Pietra M, Salamanca G, De Silva M, Canova M, Cacchione S, Castagnetti C, Bombardi C, Grandis A.Endoscopy of the guttural pouches in neonatal foals occasionally reveals atypical aspects in the course of the extracranial internal carotid arteries (ICAs) that are hardly seen in adult horses. While in veterinary medicine the data available on the presence of these anomalies in domestic mammals are limited and incomplete, in human medicine the morphological aspects of the ICAs have been the subject of extensive studies. Anomalies in the course and geometry of ICAs are commonly defined as dolichoarteriopathies, and should be classified as tortuous, kinked or coiled. To document whether the an...
Cullinane A, Newton JR.To date, equine influenza outbreaks have been reported all over the world with the exception of a small number of island nations including New Zealand and Iceland. Influenza is endemic in Europe and North America and is considered to be of potentially major economic significance to the equine industry worldwide. The importation of subclinically infected vaccinated horses, and inadequate quarantine procedures have resulted in several major outbreaks in susceptible populations for example, in Australia (2007) when more than 76,000 horses on over 10,000 properties were reported as infected. This ...
Alhassan A, Pumidonming W, Okamura M, Hirata H, Battsetseg B, Fujisaki K, Yokoyama N, Igarashi I.With the aim of developing more simple diagnostic alternatives, a differential single-round and multiplex polymerase chain reaction (PCR) method was designed for the simultaneous detection of Babesia caballi and Babesia equi, by targeting 18S ribosomal RNA genes. The multiplex PCR amplified DNA fragments of 540 and 392 bp from B. caballi and B. equi, respectively, in one reaction. The PCR method evaluated on 39 blood samples collected from domestic horses in Mongolia yielded similar results to those obtained from confirmative PCR methods that had been established earlier. Thus, the single-roun...
Ball BA.Oxidative stress is an important component of the cytopathology of equine spermatozoa undergoing storage as liquid or frozen semen. Damage to chromatin, membranes and proteins of sperm are important components of oxidative damage to sperm. Similarly, sperm are exposed to a variety of osmotic stresses during storage that result from exposure to hypertonic media or result as a consequence of osmotic changes induced during freezing. A number of changes induced during processing and storage of equine sperm also appear to induce apoptotic-like changes which may adversely affect sperm survival and f...
Bosch S, Serra Bragança F, Marin-Perianu M, Marin-Perianu R, van der Zwaag BJ, Voskamp J, Back W, van Weeren R, Havinga P.In this paper, we describe and validate the EquiMoves system, which aims to support equine veterinarians in assessing lameness and gait performance in horses. The system works by capturing horse motion from up to eight synchronized wireless inertial measurement units. It can be used in various equine gait modes, and analyzes both upper-body and limb movements. The validation against an optical motion capture system is based on a Bland-Altman analysis that illustrates the agreement between the two systems. The sagittal kinematic results (protraction, retraction, and sagittal range of motion) sh...
Bartsch S, Bauer B, Wiemann A, Clausen PH, Steuber S.Host feeding patterns of engorged sibling species of the Culicoides obsoletus and Culicoides pulicaris groups captured during three nights on two selected farms maintaining either cattle, sheep, horses, and pigs (Seedorf, Brandenburg) or cattle, sheep, moufflons, and red and fallow deer (Paulinenaue, Brandenburg) were determined by polymerase chain reaction amplification using conserved primers and sets of species-specific primers derived from vertebrates mitochondrial cytochrome b. Out of a total of 177 blood meals analysed, 115 (65%) tested positive for a blood meal from vertebrates. 63.5% (...
Takai S, Koike K, Ohbushi S, Izumi C, Tsubaki S.Antigens of Rhodococcus equi were analyzed by immunoblotting with naturally infected foal sera. Immunoblots of whole-cell antigen preparations of clinical isolates of R. equi revealed that major protein bands with molecular masses of 15 to 17 kDa were present in all clinical isolates tested and all isolates virulent for mice. In contrast, the 15- to 17-kDa antigens were not identified by immunoblotting in ATCC 6939, a type strain of R. equi that was avirulent for mice. Whole-cell antigens of 102 environmental isolates were investigated by immunoblotting and the mouse pathogenicity test. Twenty...
Field HE.Hendra virus causes acute and highly fatal infection in horses and humans. Pteropid bats (flying-foxes) are the natural host of the virus, with age and species being risk factors for infection. Urine is the primary route of excretion in flying-foxes, with viral RNA more frequently detected in Pteropus alecto and P. conspicillatus than other species. Infection prevalence in flying-foxes can vary between and within years, with a winter peak of excretion occurring in some regions. Vertical transmission and recrudescing infection has been reported in flying-foxes, but horizontal transmission is ev...
Frisbie DD, Lu Y, Kawcak CE, DiCarlo EF, Binette F, McIlwraith CW.Current autologous chondrocyte implantation (ACI) techniques require 2 surgical procedures: 1 for cell harvest and 1 for reimplantation of cultured cells. A 1-step procedure is more desirable. Objective: A 1-step surgical procedure using autologous cartilage fragments on a polydioxanone scaffold, or CAIS (cartilage autograft implantation system), in a clinically relevant defect (15-mm diameter) within equine femoral trochlea was compared with a 2-step ACI technique as well as with empty defects and defects with polydioxanone foam scaffolds alone. Methods: Controlled laboratory study. Methods: ...
Roth JA.Veterinary vaccines have had, and continue to have, a major role in protecting animal health and public health, reducing animal suffering, enabling efficient production of food animals to feed the burgeoning human population, and greatly reducing the need for antibiotics to treat food and companion animals. Prominent examples include rabies vaccines and rinderpest vaccines. Rabies vaccines for domestic animals and wildlife have nearly eliminated human rabies in developed countries. Thanks to the Global Rinderpest Eradication Program which involves vaccination, trade restrictions, and surveilla...
Julliand V, Grimm P.In the early 1990s, the equine hindgut microbial ecosystem looked like a "black box." Its vital role in hydrolyzing and fermenting fiber, thus providing energy to the host, was recognized. Although there was a critical lack of information on the hindgut microbes, their role in preventing intestinal diseases was suggested. Traditionally, the microbes of the horse hindgut were studied using culture-dependent techniques. More recently, culture-independent methods have been used and provided further insight. This review presents the history and updated knowledge regarding the microbes that live in...
Briefer EF, Maigrot AL, Mandel R, Freymond SB, Bachmann I, Hillmann E.Studying vocal correlates of emotions is important to provide a better understanding of the evolution of emotion expression through cross-species comparisons. Emotions are composed of two main dimensions: emotional arousal (calm versus excited) and valence (negative versus positive). These two dimensions could be encoded in different vocal parameters (segregation of information) or in the same parameters, inducing a trade-off between cues indicating emotional arousal and valence. We investigated these two hypotheses in horses. We placed horses in five situations eliciting several arousal level...
Rushlow K, Olsen K, Stiegler G, Payne SL, Montelaro RC, Issel CJ.The nucleotide sequence of the envelope (env) gene region of equine infectious anemia virus (EIAV), a member of the lentivirus subfamily of retroviruses, has been determined from a clone of integrated proviral DNA for which the gag and pol sequences have been reported previously. The env gene is 859 codons in length and the sequence reported here is consistent with the published biochemical properties of EIAV glycoproteins. The env gene region of EIAV shares considerable structural similarities but negligible sequence homologies with the env genes of other members of the lentivirus subfamily, ...
Keel MK, Songer JG.Clostridium difficile is a confirmed pathogen in a wide variety of mammals, but the incidence of disease varies greatly in relation to host species, age, environmental density of spores, administration of antibiotics, and possibly, other factors. Lesions vary as well, in severity and distribution within individuals, and in some instances, age groups, of a given species. The cecum and colon are principally affected in most species, but foals and rabbits develop severe jejunal lesions. Explanations for variable susceptibility of species, and age groups within a species, are largely speculative. ...
Magneson GR, Puvathingal JM, Ray WJ.The enzyme phosphoglucomutase can be used as a metal ion indicator to measure the concentrations of free Mg2+ and free Zn2+ in physiological fluids. In horse plasma, the concentration of free Mg2+ is close to 0.5 mM, whereas that of free Zn2+ is about 2 X 10(-10) M, although numerous physiological roles for Zn2+ have been postulated that would require free Zn2+ concentration orders of magnitude higher than this. A titration of plasma with Zn2+ shows that the fractional increase in free Zn2+ is essentially the same as the fractional increase in total exchangeable Zn2+, and the results are consi...
Powers AM, Oberste MS, Brault AC, Rico-Hesse R, Schmura SM, Smith JF, Kang W, Sweeney WP, Weaver SC.Venezuelan equine encephalitis (VEE) epidemics and equine epizootics occurred periodically in the Americas from the 1920s until the early 1970s, when the causative viruses, subtypes IAB and IC, were postulated to have become extinct. Recent outbreaks in Columbia and Venezuela have renewed interest in the source of epidemic/epizootic viruses and their mechanism of interepizootic maintenance. We performed phylogenetic analyses of VEE virus isolates spanning the entire temporal and geographic range of strains available, using 857-nucleotide reverse transcription-PCR products including the E3 and ...
Tell LA.Aspergillosis is relatively uncommon in mammals, but dogs, horses, cows and dolphins are susceptible. On the other hand, aspergillosis is a major cause of mortality in birds. This paper provides an overview of the manifestations of this disease in these animals. Treatment options are still lacking, but the increased incidence of the disease in humans has led to increased study, which may benefit both human and veterinary medicine.
Leroux C, Issel CJ, Montelaro RC.We have investigated the genetic evolution of three functionally distinct regions of the equine infectious anemia virus (EIAV) genome (env, rev, and long terminal repeat) during recurring febrile episodes in a pony experimentally infected with a well-characterized reference biological clone designated EIAV(PV). Viral populations present in the plasma of an EIAV(PV)-infected pony during sequential febrile episodes (18, 34, 80, 106, and 337 days postinfection) were amplified from viral RNA, analyzed, and compared to the inoculated strain. The comparison of the viral quasispecies showed that the ...
de Grauw JC, van Loon JP.Accurate recognition and quantification of pain in horses is imperative for adequate pain management. The past decade has seen a much needed surge in formal development of systematic pain assessment tools for the objective monitoring of pain in equine patients. This narrative review describes parameters that can be used to detect pain in horses, provides an overview of the various pain scales developed (visual analogue scales, simple descriptive scales, numerical rating scales, time budget analysis, composite pain scales and grimace scales), and highlights their strengths and weaknesses for po...
De Boyer Des Roches A, Richard-Yris MA, Henry S, Ezzaouïa M, Hausberger M.Lateralization of emotions has received great attention in the last decades, both in humans and animals, but little interest has been given to side bias in perceptual processing. Here, we investigated the influence of the emotional valence of stimuli on visual and olfactory explorations by horses, a large mammalian species with two large monocular visual fields and almost complete decussation of optic fibres. We confronted 38 Arab mares to three objects with either a positive, negative or neutral emotional valence (novel object). The results revealed a gradient of exploration of the 3 objects ...
Tarazona AM, Ceballos MC, Broom DM.Excessive human population growth, uncontrolled use of natural resources, including deforestation, mining, wasteful systems, biodiversity reduction by agriculture, and damaging climate change affect the existence of all animals, including humans. This discussion is now urgent and people are rethinking their links with the animals we use for clothing, food, work, companionship, entertainment, and research. The concepts of one health, one welfare, and one biology are discussed as a background to driving global change. Nothing should be exploited without considering the ethics of the action and t...
Arnhold SJ, Goletz I, Klein H, Stumpf G, Beluche LA, Rohde C, Addicks K, Litzke LF.To isolate and characterize bone marrow-derived equine mesenchymal stem cells (MSCs) for possible future therapeutic applications in horses. Methods: Equine MSCs were isolated from bone marrow aspirates obtained from the sternum of 30 donor horses. Methods: Cells were cultured in medium (alpha-minimum essential medium) with a fetal calf serum content of 20%. Equine MSC features were analyzed to determine selfrenewing and differentiation capacity. For potential therapeutic applications, the migratory potential of equine MSCs was determined. An adenoviral vector was used to determine the transdu...
Shehu-Xhilaga M, Ablan S, Demirov DG, Chen C, Montelaro RC, Freed EO.The Gag proteins of a number of different retroviruses contain late or L domains that promote the release of virions from the plasma membrane. Three types of L domains have been identified to date: Pro-Thr-Ala-Pro (PTAP), Pro-Pro-X-Tyr, and Tyr-Pro-Asp-Leu. It has previously been demonstrated that overexpression of the N-terminal, E2-like domain of the endosomal sorting factor TSG101 (TSG-5') inhibits human immunodeficiency virus type 1 (HIV-1) release but does not affect the release of the PPPY-containing retrovirus murine leukemia virus (MLV), whereas overexpression of the C-terminal portion...
Evans SV, Brayer GD.The three-dimensional structure of horse heart metmyoglobin has been refined to a final R-factor of 15.5% for all observed data in the 6.0 to 1.9 A resolution range. The final model consists of 1242 non-hydrogen protein atoms, 154 water molecules and one sulfate ion. This structure has nearly ideal bonding and bond angle geometry. A Luzzati plot of the variation in R-factor with resolution yields an estimated mean co-ordinate error of 0.18 A. An extensive analysis of the pattern of hydrogen bonds formed in horse heart metmyoglobin has been completed. Over 80% of the polypeptide chain is involv...
Tinker MK, White NA, Lessard P, Thatcher CD, Pelzer KD, Davis B, Carmel DK.A prospective study of one year was conducted on 31 horse farms to obtain population based estimates of incidence, morbidity and mortality rates of equine colic. Farms with greater than 20 horses were enrolled by randomly selecting horse owners from 2 adjacent counties of Virginia and Maryland. Descriptive information for 1427 horses was collected at the initiation of the study and updated at 3 month intervals. Time on the farm during the study was tabulated for each horse. When colic was reported by the owner, investigators visited the farm to obtain information about the colic. The crude inc...
Burn CC, Dennison TL, Whay HR.Over 85% of equids live in developing countries, where they are usually used for work. We aimed to identify environmental factors associated with potential welfare problems in working equids, helping target welfare interventions towards the most vulnerable animals. Over 5 years, non-invasive behavioural and physical data were collected from 5481 donkeys, 4504 horses, and 858 mules across nine developing countries. Hoof and limb problems were over 90% prevalent, and 85% of equids were thin. Older, thinner animals had the most problems, and species differences in susceptibility were found. Equid...
Hitchens PL, Morrice-West AV, Stevenson MA, Whitton RC.Studies of racehorse injury or fatality in various countries have identified common, and in some cases conflicting, risk factors. We conducted a systematic search of the relevant literature published from 1990 to 2017. Peer-reviewed articles were included if they reported the incidence of fatal or catastrophic musculoskeletal injury (CMI) in Thoroughbred flat races (n=21) or risk factors for CMI (n=65). Pooled effect sizes were estimated using the random-effects DerSimonian-Laird model. The pooled incidence of CMI was 1.17 (95% confidence interval 0.90, 1.44) per 1000 race starts. Almost 300 f...
Wathan J, Burrows AM, Waller BM, McComb K.Although previous studies of horses have investigated their facial expressions in specific contexts, e.g. pain, until now there has been no methodology available that documents all the possible facial movements of the horse and provides a way to record all potential facial configurations. This is essential for an objective description of horse facial expressions across a range of contexts that reflect different emotional states. Facial Action Coding Systems (FACS) provide a systematic methodology of identifying and coding facial expressions on the basis of underlying facial musculature and mus...
