Analyze Diet

Topic:Digestibility

Digestibility in horses refers to the process by which horses break down and absorb nutrients from their feed. It involves the mechanical and chemical breakdown of feed components in the digestive tract, enabling nutrient absorption into the bloodstream. Digestibility is influenced by various factors, including feed type, processing methods, and individual horse health. Commonly examined components include fiber, protein, starch, and fat, each requiring specific digestive processes. Research in this area often focuses on optimizing feed formulations and understanding the digestive efficiency of different feedstuffs. This page compiles peer-reviewed research studies and scholarly articles that explore the mechanisms of digestion, factors affecting digestibility, and implications for equine nutrition and health.
Effects of two equine digestive aid supplements on hindgut health.
Translational animal science    September 10, 2018   Volume 3, Issue 1 340-349 doi: 10.1093/tas/txy103
Johnson ACB, Rossow HA.Gastrointestinal disease is the number one killer of horses. Little is known about the maintenance of microbes in the equine hindgut and how to distinguish a healthy gut in a live horse. Utilization of internal and external digestibility markers and starch fermentation has been extensively studied in ruminants and is the basis for research conducted on horses. The aims of this study were to investigate the effects of two equine feed digestive aid supplements on hindgut health () as reflected in fecal pH and digestibility and to compare and validate DM digestibility measurements through the use...
Evaluation of titanium dioxide and chromic oxide as digestibility markers in ponies fed alfalfa hay in relation to marker dosing frequency.
Animal : an international journal of animal bioscience    September 3, 2018   Volume 13, Issue 4 702-708 doi: 10.1017/S1751731118002112
Schaafstra FJWC, van Doorn DA, Schonewille JT, van den Boom R, Verschuur M, Blok MC, Hendriks WH.In equines, Cr2O3 is widely accepted as an indigestible marker, but there are health concerns regarding the carcinogenic properties of Cr2O3. Recently, TiO2 has been suggested to be an alternative digestibility marker in equines. However, a comparison between Cr2O3 and TiO2 has not been made in equines. Six Welsh pony geldings (initial BW: 254±3 kg; 7 years of age) fed chopped alfalfa hay were used to evaluate the use of TiO2 (Ti) and Cr2O3 (Cr) as markers for calculating apparent digestibility and to investigate the effect of frequency of marker administration on the measurement of digestibi...
Effect of exercise on apparent total tract digestibility of nutrients and faecal recovery of ADL and TiO2 in ponies.
Animal : an international journal of animal bioscience    March 7, 2018   Volume 12, Issue 12 2505-2510 doi: 10.1017/S1751731118000356
Schaafstra FJWC, van Doorn DA, Schonewille JT, Roelfsema E, Westermann CM, Dansen O, Jacobs M, Lee JY, Spronck EA, Blok MC, Hendriks WH.Exercise and physical training are known to affect gastrointestinal function and digestibility in horses and can lead to inaccurate estimates of nutrient and energy digestibility when markers are used. The effect of exercise on apparent nutrient digestibility and faecal recoveries of ADL and TiO2 was studied in six Welsh pony geldings subjected to either a low- (LI) or high-intensity (HI) exercise regime according to a cross-over design. Ponies performing LI exercise were walked once per day for 45 min in a horse walker (5 km/h) for 47 consecutive days. Ponies submitted to HI exercise were gra...
Effect of Dental Correction on Fecal Fiber Length in Horses.
Journal of equine veterinary science    February 28, 2018   Volume 64 77-80 doi: 10.1016/j.jevs.2018.02.016
Di Filippo PA, Vieira V, Rondon DA, Quirino CR.The aim of the study was to determine if dental correction changes fecal fiber length (FFL) in horses that had never been subjected to dental treatments. Thirty adult horses with mild to moderate dental abnormalities were selected. Dental disorders noted included sharp enamel points, focal overgrowths, shear mouth, step mouth, wave mouth, accentuated transverse ridges, and diastema. The horses were confined to individual stalls and fed Tifton hay (Cynodon spp.) 1.5% bwt/d, divided equally between two feedings. Fecal samples were taken for FFL analysis before (Day 0) and after (Day 15) dental c...
Influences of different dietary contents of macrominerals on the availability of trace elements in horses.
Journal of animal physiology and animal nutrition    October 13, 2017   Volume 102, Issue 2 e633-e640 doi: 10.1111/jpn.12805
Neustädter LT, Kamphues J, Ratert C.In this study, influences of a reduced macromineral intake on the trace element metabolism in horses at maintenance were investigated. Background of this study is the revised recommendation on the macromineral supply for horses (GfE ). Balance studies on three adult pony geldings with body weights of 405 / 348 / 384 kg were performed to obtain data on apparent digestibility (aD), retention and serum concentrations of different trace elements (Cu, Zn, Se) at different dietary macromineral levels. A mineral supplement or a complementary feed-with a reduced macromineral content-was added to a ha...
Comparative selective retention of particle size classes in the gastrointestinal tract of ponies and goats.
Journal of animal physiology and animal nutrition    July 11, 2017   Volume 102, Issue 2 429-439 doi: 10.1111/jpn.12763
Hummel J, Scheurich F, Ortmann S, Crompton LA, Gerken M, Clauss M.There is a discrepancy in the literature on potential digesta separation mechanisms in horses, with both a selective retention of fine and of large particles postulated in different publications. To assess the net effect of such mechanisms, we fed ponies on a hay-only diet a pulse dose of whole (unchopped) marked hay together with a solute marker, collected faeces on a regular basis, measured marker concentrations in whole faeces and in their large (2.0-16 mm), medium (0.5-1.0 mm) and small (0.063-0.25 mm) particle fraction, and calculated the corresponding mean retention times (MRTs). For ...
Feed intake patterns of sport ponies and warmblood horses following iso-energetic intake of pelleted fibre-rich mixed feed, muesli feed and semicrushed oat grains.
Journal of animal physiology and animal nutrition    June 20, 2017   Volume 101 Suppl 1 37-42 doi: 10.1111/jpn.12732
Bochnia M, Boesel M, Bahrenthien L, Wensch-Dorendorf M, Zeyner A.The aim of the study was to compare diverse feed intake patterns in sport ponies and warmblood-type horses after feeding iso-energetic amounts of three different concentrates: a pelleted fibre-rich mixed feed (PF), a muesli feed (MF) and semicrushed oat grains (OG). Four sport ponies and six warmblood-type horses received the concentrates and meadow hay according to maintenance (0.52 MJ metabolizable energy (ME) kg body weight (BW) /day). Both breeds were allocated at random to three groups and received once daily for 8 days either OG (1 g starch/kg BW/meal) or iso-energetic quantities of ...
Evaluation of methodological aspects of digestibility measurements in ponies fed different haylage to concentrate ratios.
Animal : an international journal of animal bioscience    April 17, 2017   Volume 11, Issue 11 1922-1929 doi: 10.1017/S1751731117000751
Schaafstra FJWC, van Doorn DA, Schonewille JT, van Riet MMJ, Visser P, Blok MC, Hendriks WH.Methodological aspects of digestibility measurements were studied in four Welsh pony geldings consuming haylage-based diets with increasing proportions of a pelleted concentrate according to a 4×4 Latin square design experiment. Ponies were fed four experimental, iso-energetic (net energy (NE) basis) diets (i.e. 22 MJ NE/day) with increasing proportions of a pelleted concentrate (C) in relation to haylage (H). The absolute amounts of diet dry matter fed per day were 4.48 kg of H (100H), 3.36 and 0.73 kg of H and C (75H25C), 2.24 and 1.45 kg of H and C (50H50C) and 1.12 and 2.17 kg of H and C ...
Comparative fibre-degrading capacity in foals at immediate and late post-weaning periods.
Animal : an international journal of animal bioscience    February 21, 2017   Volume 11, Issue 9 1497-1504 doi: 10.1017/S1751731117000349
Faubladier C, Julliand V, Beuneiche L, Philippeau C.Total tract apparent digestibility of dietary fibrous components and parameters of the faecal digestive ecosystem were compared at immediate and late post-weaning periods using five foals. The foals were abruptly weaned (day 0) from their mares at 6 months of age. Immediately (day 0 to day 14) and later (day 131 to day 194) after weaning, foals received the same basal diet consisting of hay and pellets. Pellets were fed at 0.36% BW. Hay was offered ad libitum and corresponded to 120% of the average voluntary hay intake measured individually during the immediate (days 1 to 4) and late (days 180...
Effects of Different Oral Doses of Sodium Chloride on the Basal Acid-Base and Mineral Status of Exercising Horses Fed Low Amounts of Hay.
PloS one    January 3, 2017   Volume 12, Issue 1 e0168325 doi: 10.1371/journal.pone.0168325
Zeyner A, Romanowski K, Vernunft A, Harris P, Müller AM, Wolf C, Kienzle E.The provision of NaCl, according to current recommendations, to horses in moderate work has been shown to induce immediate postprandial acidosis. The present study aimed to clarify whether this NaCl induced acidosis i) persists beyond the immediate postprandial period, and ii) is still present after a 2 week adaptation period. Six adult warmblood mares in moderate work received daily 1.00 kg hay per 100 kg body weight (bwt) only together with 0.64 kg unprocessed cereal grains/100 kg bwt.d as fed basis. Using a 3x3 Latin Square, either 0 (NaCl-0), 50 (NaCl-50) or 100 (NaCl-100) g NaCl/d were fe...
Allergenicity of milk of different animal species in relation to human milk.
Postepy higieny i medycyny doswiadczalnej (Online)    December 31, 2016   Volume 70 1451-1459 doi: 10.5604/17322693.1227842
Pastuszka R, Barłowska J, Litwińczuk Z.Protein content in cow milk (with over 20 proteins, and peptides may also occur as a result of enzymatic hydrolysis) ranges from 2.5% to 4.2% and is about 1.5-2 times higher than in human milk. Its most important allergens are considered to be β-lactoglobulin (absent in human milk) and αs1-casein. The most similar in composition to human milk is horse and donkey milk. It contains considerably more whey proteins (35-50%) than cow milk (about 20%), and the concentration of the most allergenic casein fraction αs1 is 1.5-2.5 g/l. In comparison, the content of αs1-casein in cow milk is about 10...
Review: Feeding conserved forage to horses: recent advances and recommendations.
Animal : an international journal of animal bioscience    November 24, 2016   Volume 11, Issue 6 958-967 doi: 10.1017/S1751731116002469
Harris PA, Ellis AD, Fradinho MJ, Jansson A, Julliand V, Luthersson N, Santos AS, Vervuert I.The horse is a non-ruminant herbivore adapted to eating plant-fibre or forage-based diets. Some horses are stabled for most or the majority of the day with limited or no access to fresh pasture and are fed preserved forage typically as hay or haylage and sometimes silage. This raises questions with respect to the quality and suitability of these preserved forages (considering production, nutritional content, digestibility as well as hygiene) and required quantities. Especially for performance horses, forage is often replaced with energy dense feedstuffs which can result in a reduction in the p...
Black rhinoceros (Diceros bicornis) and domestic horse (Equus caballus) hindgut microflora demonstrate similar fermentation responses to grape seed extract supplementation in vitro.
Journal of animal physiology and animal nutrition    November 13, 2016   Volume 101, Issue 5 e195-e209 doi: 10.1111/jpn.12587
Huntley NF, Naumann HD, Kenny AL, Kerley MS.The domestic horse is used as a nutritional model for rhinoceros maintained under human care. The validity of this model for browsing rhinoceros has been questioned due to high prevalence of iron overload disorder (IOD) in captive black rhinoceros (Diceros bicornis), which is associated with high morbidity and mortality. Iron chelators, such as tannins, are under investigation as dietary supplements to ameliorate or prevent IOD in prone species. Polyphenolic compounds variably affect microbial fermentation, so the first objective of this experiment was to evaluate the effects of grape seed ext...
HORSE SPECIES SYMPOSIUM: Can the microbiome of the horse be altered to improve digestion?
Journal of animal science    June 11, 2016   Volume 94, Issue 6 2275-2281 doi: 10.2527/jas.2015-0056
Coverdale JA.Intensive management practices in the horse industry present a unique challenge to the microbiome of the large intestine. Common management practices such as high-concentrate diets, low forage quality, meal feeding, and confinement housing have an impact on intestinal function, specifically large intestinal fermentation. The microbiome of the equine large intestine is a complex and diverse ecosystem, and disruption of microbiota and their environment can lead to increased incidence of gastrointestinal disorder. Digestion in the horse can be improved through a variety of approaches such as feed...
Phosphorus digestibility and phytate degradation by yearlings and mature horses.
Journal of animal science    December 8, 2015   Volume 93, Issue 12 5735-5742 doi: 10.2527/jas.2015-9139
Fowler AL, Hansen TL, Strasinger LA, Harlow BE, Lawrence LM.Inorganic P is often added to growing horse diets because organic P, or phytate-P, is believed to have lower digestibility. If horses can efficiently digest organic P, then the need for inorganic P may be reduced. Much of the P in grain-based concentrates fed to growing horses is in the form of phytate-P. Little is known about the ability of growing horses to degrade phytate-P or whether horse age affects mineral digestion in horses. The objective of this study was to examine the effect of age on P, Ca, and Mg digestibility as well as phytate-P degradation. Four yearling geldings and 4 mature ...
Evaluation of methodological aspects of digestibility measurements in ponies fed different grass hays.
Journal of animal science    November 3, 2015   Volume 93, Issue 10 4742-4749 doi: 10.2527/jas.2015-9240
Schaafstra FJ, van Doorn DA, Schonewille JT, Wartena FC, Zoon MV, Blok MC, Hendriks WH.Methodological aspects of digestibility measurements of feedstuffs for equines were studied in four Welsh pony geldings consuming four grass-hay diets in a 4 × 4 Latin square design. Diets contained either a low (L), medium (M), high (H), or very high (VH) ADF content (264, 314, 375, or 396 g·kg DM, respectively). Diets were supplemented with minerals, vitamins, and TiO (3.9 g Ti·d). Daily feces excreted were collected quantitatively over 10 consecutive days and analyzed for moisture, ash, ADL, AIA, and titanium (Ti). Minimum duration of total fecal collection (TFC) required for an accurate...
Impact of barley form on equine total tract fibre digestibility and colonic microbiota.
Animal : an international journal of animal bioscience    August 28, 2015   Volume 9, Issue 12 1943-1948 doi: 10.1017/S1751731115001524
Philippeau C, Sadet-Bourgeteau S, Varloud M, Julliand V.This study aimed at assessing the impact of four barley forms on total tract apparent digestibility of dietary fibre in horses fed a large amount of starch in the morning meal (0.27% BW). Processed barley forms had a greater pre-caecal starch digestibility than the whole form. Based on this result, we hypothesised that using barley-processing methods would limit the potential dumping of undegraded starch in the hindgut of horses and, consequently, the potential negative effect on fibre degradation in the hindgut. In a 4×4 latin square design, four mature geldings fitted with a right ventral c...
Considerations for the use of restricted, soaked grass hay diets to promote weight loss in the management of equine metabolic syndrome and obesity.
Veterinary journal (London, England : 1997)    July 30, 2015   Volume 206, Issue 2 170-177 doi: 10.1016/j.tvjl.2015.07.027
Argo CM, Dugdale AH, McGowan CM.The addition of hay soaking to current nutritional advice for weight loss management for equine obesity lacks clinical evidence. Twelve overweight/obese horses and ponies were used to test the hypothesis that feeding soaked hay at 1.25% of body mass (BM) daily as dry matter (DM) before soaking would elicit weight losses within the target 0.5-1.0% of BM weekly. Six animals were used to evaluate the impact of nutrient-leaching on the digestibility and daily intakes of dietary energy and nutrients. Soaked hay DM was corrected in accordance with the 'insoluble' ADF content of fresh and soaked hays...
Application of long-chain alcohols as faecal markers to estimate diet composition of horses and cattle fed with herbaceous and woody species.
Animal : an international journal of animal bioscience    July 10, 2015   Volume 9, Issue 11 1786-1794 doi: 10.1017/S1751731115001196
López López C, Celaya R, Santos AS, Rodrigues MA, Osoro K, Ferreira LM.Utilization of long-chain alcohols (LCOH) as diet composition markers in horses and cattle was assessed in a study conducted with 12 mature crossbreed mares (385±47 kg BW) and six adult non-lactating cows (499±36 kg BW) of Asturiana de los Valles breed. The LCOH data were combined with alkane and long-chain fatty acid (LCFA) data to test the applicability of combining these markers to estimate diet composition. Animals were randomly divided into groups of three animals and received a daily total amount of 1.0 kg dry matter/100 kg BW of diets composed of different proportions of ryegrass (Lol...
Daily methane production pattern of Welsh ponies fed a roughage diet with or without a cereal mixture.
Journal of animal science    May 29, 2015   Volume 93, Issue 4 1916-1922 doi: 10.2527/jas.2014-8415
Dansen O, Pellikaan WF, Hendriks WH, Dijkstra J, Jacobs MP, Everts H, van Doorn DA.Methane production from Welsh ponies fed 2 isoenergetic diets (NE basis) at maintenance was studied in a crossover design with 4 mature geldings (230 ± 10.5 kg BW, mean ± SE). Treatments included a roughage-only (R) diet (5.1 kg DM/d) or a roughage plus cereal mix (RC) diet (2.5 kg DM hay/d plus 1.1 kg DM cereal mix/d). For both diets, the same grass hay was used (898 g DM/kg and 4.5 MJ NE/kg DM) and a commercial cereal mix was used in the RC diet (890 g DM/kg and 9.6 MJ NE/kg DM). Ponies were housed in pairs in climate-controlled respiration chambers. Carbon dioxide production (CO2), oxygen...
Energy supplementation and herbage allowance effects on daily intake in lactating mares.
Journal of animal science    May 29, 2015   Volume 93, Issue 5 2520-2529 doi: 10.2527/jas.2014-8447
Collas C, Dumont B, Delagarde R, Martin-Rosset W, Fleurance G.Little is known about how to manage grazing horses, including the thresholds under which energy supplementation is required. Here we investigated the effects of daily herbage allowance (DHA) and energy supplementation (ES) on daily herbage intake in lactating mares of light breeds grazing high-quality regrowth during summer. Three contrasting DHA, low (LOW), medium (MED), and high (HIGH), that is, 35.0, 52.5, and 70.0 g DM∙kg BW(-1)∙d(-1), respectively, were obtained by adjusting pasture strip width. Eighteen Anglo-Arab and French Saddle lactating mares were either supplemented with 2.6 kg...
Organic matter and macromineral digestibility in domestic rabbits (Oryctolagus cuniculus) as compared to other hindgut fermenters.
Journal of animal physiology and animal nutrition    April 9, 2015   Volume 99, Issue 6 1197-1209 doi: 10.1111/jpn.12323
Hagen KB, Tschudin A, Liesegang A, Hatt JM, Clauss M.It is generally assumed that animal species differ in physiological characteristics of their digestive tract. Because investigating digestive processes is often labour-intensive, requiring lengthy adaptation and collection periods, comparisons are often made on the basis of data collated from different studies. We added a new data set on dietary crude fibre (CF) and macromineral (Na, K, Ca, P, Mg) composition and apparent digestibility (aD) of organic matter (OM) and macrominerals in rabbits (Oryctolagus cuniculus; a total of 180 measurements with 12 individuals and 10 different diets) to a li...
A new protein evaluation system for horse feed from literature data.
Journal of nutritional science    February 4, 2015   Volume 4 e4 doi: 10.1017/jns.2014.66
Zeyner A, Kirchhof S, Susenbeth A, Südekum KH, Kienzle E.Few data on apparent pre-caecal digestibility (APCD) of crude protein (CP) and particularly amino acids (AA) are available from studies with horses. Protein bound in cell walls (i.e. neutral detergent insoluble CP (NDICP)) is unlikely to be decomposed by digestive enzymes in the small intestine. In contrast the corresponding analytical fraction of neutral detergent soluble CP (NDSCP) (NDSCP = CP-NDICP) is likely to be available for auto-enzymatic digestion. A literature analysis on the relationship between NDICP/NDSCP and pre-caecal indigestible/digestible CP was carried out. There was a str...
The impact of dietary protein levels on nutrient digestibility and water and nitrogen balances in eventing horses.
Journal of animal science    January 9, 2015   Volume 93, Issue 1 229-237 doi: 10.2527/jas.2014-6971
Oliveira CA, Azevedo JF, Martins JA, Barreto MP, Silva VP, Julliand V, Almeida FQ.This study was performed to evaluate the impact of dietary protein levels on nutrient digestibility and water and nitrogen balances in conditioning eventing horses. Twenty-four Brazilian Sport Horses, male and female (8.0 to 15.0 yr; 488 ± 32 kg BW), were used in a randomized design with 4 levels of CP diets: 7.5%, 9.0%, 11.0%, and 13.0%. A digestion assay was performed with partial feces collection over 4 d, followed by 1 d of total urine collection. Data were submitted to regression analysis and adjusted to linear and quadratic models (P < 0.05). No differences were observed in the intake o...
The effect of dietary carbohydrate composition on apparent total tract digestibility, feed mean retention time, nitrogen and water balance in horses.
Animal : an international journal of animal bioscience    July 14, 2014   Volume 8, Issue 11 1788-1796 doi: 10.1017/S175173111400175X
Jensen RB, Austbø D, Bach Knudsen KE, Tauson AH.A total of four diets with different carbohydrate composition were investigated in a 4×4 Latin square design experiment with four Norwegian Coldblooded trotter horses. The objective of the present study was to increase the fermentable fibre content and reduce the starch intake of the total ration obtained by partly substituting mature hay and barley with sugar beet pulp (SBP), a soluble fibre source. The diets investigated were hay only (HAY), hay (85% of dry matter intake (DMI)) and molassed SBP (15% of DMI) (SBP), hay (68% of DMI) and barley (32% of DMI) (BAR), and hay (68% of DMI), barley ...
Nitrogen digestion and urea recycling in Hokkaido native horses fed hay-based diets.
Animal science journal = Nihon chikusan Gakkaiho    July 12, 2014   Volume 86, Issue 2 159-165 doi: 10.1111/asj.12256
Obitsu T, Hata H, Taniguchi K.Nitrogen (N) digestion and urea-N metabolism in Hokkaido native horses fed roughage-based diets containing different types and levels of protein sources were studied. Horses (173 ± 4.8 kg) fitted with an ileum cannula were fed four diets consisting of 100% timothy hay (TH), 88% TH and 12% soybean meal (SBM), 79% TH and 21% SBM, and 51% TH and 49% alfalfa hay at 2.2% of body weight. Dietary protein content varied from 5% to 15% of dry matter. Apparent N digestibilities in the pre-cecum and total tract for the TH diet were lower than those for other diets. However, the proportion of post-ileum ...
Fibre digestibility, abundance of faecal bacteria and plasma acetate concentrations in overweight adult mares.
Journal of nutritional science    May 7, 2014   Volume 3 e10 doi: 10.1017/jns.2014.8
Shepherd ML, Ponder MA, Burk AO, Milton SC, Swecker WS.The purpose of the present study was to compare digestibility of grass hay, faecal and plasma volatile fatty acid (VFA) concentrations, and faecal bacterial abundance in overweight and moderate-condition mares. Five overweight adult mixed-breed mares and five adult mixed-breed mares in moderate condition were housed individually and limit-fed orchard grass (Dactylis glomerata) hay at 20 g/kg body weight (as fed) daily for 14 d. Forage DM and fibre digestibility were determined using AOAC methods; digestible energy was measured using bomb calorimetry; plasma and faecal VFA concentrations were ...
Impact of water-soaking on the nutrient composition of UK hays.
The Veterinary record    March 27, 2014   Volume 174, Issue 18 452 doi: 10.1136/vr.102074
Mack SJ, Dugdale AH, Argo CM, Morgan RA, McGowan CM.No abstract available
Mobile bag starch prececal disappearance and postprandial glycemic response of four forms of barley in horses.
Journal of animal science    March 25, 2014   Volume 92, Issue 5 2087-2093 doi: 10.2527/jas.2013-6850
Philippeau C, Varloud M, Julliand V.To determine prececal starch digestibili-ty and estimate glucose uptake from the digestion of 4 forms of barley in the small intestine, 4 mature cecally fistulated geldings (449 ± 41 kg BW) fed a 62:38 (wt/wt) meadow hay:concentrate diet at 1.7 kg DM/100 kg BW were included in a 4 × 4 Latin square design experiment. During each period, horses received 80% DM of their concentrate as 1 of the 4 forms of a same batch of barley, whole grain, 2.5 mm ground, steam flaked, and pelleted. Hay was offered in 2 equal meals and concentrate in 2 unequal meals. The starch supply in the morning meal amount...
Effect of grass hay intake on fiber digestion and digesta retention time in the hindgut of horses.
Journal of animal science    March 18, 2014   Volume 92, Issue 4 1574-1581 doi: 10.2527/jas.2013-6676
Miyaji M, Ueda K, Hata H, Kondo S.Eight Thoroughbred horses were used to examine the effects of grass hay intake on the fiber digestion and the retention time of digesta in the total gastrointestinal tract and the hindgut segments. The horses were randomly assigned to 2 groups and offered 2.0 (high intake [HI]) or 1.3 kg DM/(100 kg BW • d; low intake [LI]) of timothy hay in equal amounts every 3 h for 17 d. The digestibility and total tract mean retention time of digesta (tMRT) in the total gastrointestinal tract were measured from d 11 to d 15. To measure the mean retention time of digesta in each hindgut segment (sMRT), th...