Equids are members of the family Equidae, which includes modern horses, donkeys, and zebras, as well as extinct species. Equids are characterized by their long limbs, single-toed hooves, and herbivorous diet, which is primarily composed of grasses. Horses, specifically, have been domesticated for thousands of years and have played significant roles in agriculture, transportation, and sport. This page compiles peer-reviewed research studies and scholarly articles that explore various aspects of equids and horses, including their evolution, physiology, behavior, and interactions with humans. The collected works provide insights into the genetic diversity, adaptive traits, and conservation efforts related to equid species.
Pereira DIB, Botton SA, Ianiski LB, Braga CQ, Maciel AF, Melo LG, Zambrano CG, Bruhn FRP, Santurio JM.This systematic review compiles reports of clinical pythiosis in horses, mules and donkeys from 1960 to 2023 worldwide, focusing on Brazil. We searched databases and included 71 articles detailing clinical characteristics, geographic distribution, epidemiology, diagnostic methods, therapies, and outcomes. The results showed that publications on equine pythiosis have significantly increased since 2010. Brazil reported the highest incidence, comprising 55% of cases, predominantly in the southern, northeastern, and central-western regions during summer and autumn. Cutaneous pythiosis was the most...
Núñez-Montero K, Leal K, Rojas-Villalta D, Castro M, Larronde C, Wagenknecht L, Contreras MJ.Studies on the bacterial composition of seminal samples have primarily focused on species isolated from semen and their effects on fertility and reproductive health. Culture-independent techniques, such as 16S rRNA gene sequencing and shotgun metagenomics, have revolutionized our ability to identify unculturable bacteria, which comprise >90% of the microbiome. These techniques allow for comprehensive analysis of microbial communities in seminal samples, shedding light on their interactions and roles. In this study, we characterized the taxonomic diversity of seminal microbial communities in he...
Hisaeda K, Ono T, Kadekaru S, Hata A, Miyama TS, Kutara K, Sugimoto K, Hiasa Y, Ohzawa E, Kunieda T, Iwata E, Kitagawa H.Plasma or serum amino acids are used to evaluate nutritional status and metabolic disorders. In this study, we aimed to set reference values of serum amino acid concentrations in the Noma horse, a Japanese native horse. Thirty-one horses were classified into six age groups: neonatal foal (0-4 days), foal (0.5-1 years), youth (5 years), middle age (10 years), old (15 years), and extra-old (>20 years). Horses >5 years of age were analyzed together as the adult group. In the adult horses, there were no significant differences among the serum amino acid concentrations of each age group. The ...
Pereira LO, DE Souza AF, Spagnolo JD, Yamada ALM, Salgado DMRA, DE Zoppa ALDV.Osteoarthritis (OA) is a prevalent condition in horses, leading to changes in trabecular bone structure and radiographic texture. Although fractal dimension (FD) and lacunarity have been applied to quantify these changes in humans, their application in horses remains nascent. This study evaluated the use of FD, bone area fraction (BA/TA), and lacunarity in quantifying trabecular bone differences in the proximal phalanx (P1) in 50 radiographic examinations of equine metacarpophalangeal joints with varying OA degrees. In the dorsopalmar view, regions of interest were defined in the trabecular bo...
Graham-Thiers P, Bowen K.Eight geldings weighing 544 ± 16 kg were used to evaluate feeding a postexercise protein meal on plasma amino acids during recovery. Horses were fed sweet feed, corn, grass hay and equal amounts of a protein pellet (32% CP) with meals (MP group) or postexercise (EP group). Horses exercised 1-2 h/day, 5 days/week, for 12 weeks. A pre and poststudy 4 days total urine and feces collection was conducted. Urine and fecal samples were analyzed for nitrogen (N) to calculate N balance. Blood samples were drawn immediately after and at 1 and 3 h postexercise at the start and end of the stud...
Elghryani N, Lawlor A, McOwan T, de Waal T.Over the preceding decades, the widespread dependence on anthelmintic drugs for managing nematodes in grazing equids has given rise to resistance against commonly used anthelmintics in various countries. This study explores the prevalence of anthelmintic resistance across 44 horse farms in Ireland. Anthelmintic efficacy was evaluated through fecal egg count reduction (FECR) tests employing the mini-FLOTAC technique. Resistance to benzimidazoles was identified in 12 out of 14 farms (FECR range: 0.00% to 86.2%). Ivermectin resistance was observed on two farms, one with an FECR of 80.70% and anot...
Janeiro-Otero A, Álvarez X, Dormann CF.Galician forests in northwestern Spain are subject to frequent wildfires with high environmental and economic costs. In addition, due to the consequences of climate change, these fires are becoming more virulent, occurring throughout the year, and taking place in populated areas, in some cases involving the loss of human life. Therefore, forest fire prevention is even more relevant than mitigating its consequences. Given the costs involved in forestry work, alternative measures to reduce fuel load and create vegetation gaps are needed. One involves grazing by an endemic species of feral horses...
Safaee Firouzabadi MS, Paidar Ardakani A.Many studies have shown a strong correlation between Hindgut Acidosis (HGA) and the occurrence of laminitis in horses; therefore, the early diagnosis of HGA is essential. In this study, we investigated changes in the plasma concentrations of lipopolysaccharide-binding protein (LBP) and serum amyloid A (SAA) as inflammatory markers in horses with laminitis. Sixteen healthy male Arabian horses that had cecal cannulation without visible laminitis or general symptoms were randomly divided into two groups. The horses were fed two different diets in a forage-to-concentrate ratio. Blood samples were ...
Price HA, Logan AA, Snyder AJ, Higgins AH.Intercollegiate Horse Show Association (IHSA) competitions evaluate riders on equitation and skills when riding randomly drawn horses. This study considered demographic and competition-related variables of horses involved in IHSA competitions, and their correlation to performance through points accumulated. Demographics were recorded for fifty-nine horses in two-day Hunter Seat and Western IHSA shows along with use of artificial riding aids, day of competition, number of rides, and points, to evaluate performance. Data were analyzed using a mixed effect model and correlation coefficients with ...
Machado M, Queiroz-Machado CRR, Queiroz F, Gardner DR, Riet-Correa F.A Mimosa pudica var. unijuga-associated toxicity affecting horses occurred in Araguari, Triângulo Mineiro, Southeast Brazil. Affected horses had gradual hair loss of the mane and tail and endocrine dermatosis after grazing for three months during the dry season on a paddock invaded by the plant. The main histological lesions include compact ortho-keratotic hyperkeratosis and numerous flame follicles. Toxicological analysis by HPLC-UV demonstrated 0.8 mg/g of mimosine in the leaves.
Lima AE, Piñeros DDV, Alibrando BB, Bittar MJ, de Souza AF, Mendes RP, de Freitas SH, Bernardino T, Corrêa RR.The anatomical variations of the maxillary septum and the septal bullae can generate challenges during the interpretation of radiographs of the horses' heads and make it difficult to accurately identify the sites affected in sinus disorders. The description of the radiographic appearance of these structures is currently scarce in the scientific literature. This work aims to describe the anatomical and radiographic characteristics of the maxillary septum and maxillary septal bullae in horses. Six chemically preserved equine cadaver heads were used which, after being submitted to the maxillary o...
Medina B AL, Faleiros RR, Martínez A JR.Equine Gastric Ulcer Syndrome (EGUS) occurs with variable prevalence in horses, donkeys, and mules. Due to the particularities of the mucous membranes, the syndrome is made up of Squamous Gastric Disease (ESGD) and Glandular Gastric Disease (EGGD). Given the multifactorial nature and multiple classification systems of the syndrome, significant differences have been reported between prevalence studies performed ante mortem, which are even more remarkable when compared with postmortem evaluations. This study aimed to determine the presence and grade of squamous gastric disease in horses, donkeys...
Discepolo DR, Gaare E, Handlos G, Perry EB.Cutaneous pH and transepidermal water loss (TEWL) are commonly utilized measures in dermatological research as they provide information concerning barrier function. The importance of dermal health has become more evident in recent years. Accordingly, the aim of this work was to identify natural fluctuations in the biophysical parameters of healthy equine skin. Cutaneous pH and TEWL was collected on nine mares at 6:00 AM, 12:00 PM, and 6:00 PM daily for five days on the nose, withers, girth area, mid-back, and base of tail. Ambient temperature and humidity were measured at each collection. Stat...
Stellmack JM, Logan AA, Higgins AH, Hoffman RM.Periods of limited activity during semester break may reduce performance during return to ridden work. This study evaluated fitness and muscling of horses when returning to work, following a 12-week period during which horses either continued (conditioned) or discontinued (non-conditioned) ridden work. It was hypothesized that non-conditioned horses would have a lower level of fitness, resulting in higher resting and peak heart rates and lower levels of muscling. Twelve mature, stock type horses aged 16 ± 5 years were assigned to either a conditioned group that maintained light-to-moderate ri...
Nunes MM, Gurgel JRC, Munhoz ML, de Giuli V, Carneiro GF, Miragaya MH, Gallelli MF, Guimaraes JD.The aim of this study was to assess the effect of lyophilized freezing extenders, which can be stored at room temperature, on stallion post-thaw sperm total motility (TM). Ejaculates of 28 stallions were frozen with four different extenders: two commercial freezing extenders offered worldwide and two novel lyophilized extenders (STAR and MX3), and two different cryopreservation protocols (CP1 with an equilibration period of 20 min. and CP2 with an equilibration period of 60 min.). The TM was assessed after thaw. Mean TM did not show significant differences between cryopreservation protocols wi...
Ribeiro M, Gomes-Gonçalves S, Moreira G, Cardoso L, Mesquita JR.The life-cycle of the parasitic forms of Halicephalobus gingivalis is yet to be fully understood. In cases where there is evidence to support the presence of eggs from this parasite or its DNA in the faeces of equines, a thorough investigation of the clinical status and gastro-intestinal tract of the affected animals is warranted, as well as detailed descriptions of the employed coprological technique. Since reports of the identification of H. gingivalis eggs in faeces are sparse, objective measurements and high-quality images must be provided, in order to ensure optimal criteria for classific...
Lindinger H, Wehrend A.In horses, parturition is characterized by the fact that any delays may rapidly result in the death of the foal. Therefore, birth monitoring and the rapid detection of dystocia are of great importance. For this purpose, the differentiation between behavior associated with physiological parturition and activities related to difficult parturition is important. The aim of this publication is to present the physiological behavior of mares during parturition on the basis of a literature review. The behavior of mares in the opening phase of parturition is highly individual. In general, it is charact...
Aragona F, Rizzo M, Arfuso F, Acri G, Fazio F, Piccione G, Giannetto C.The aim of the present study was to investigate eye temperature modifications after road transport in athletic horses habituated to travel. Eight adult Italian saddle horses traveled 100 km and, two weeks later, 300 km. Eye temperature (ET), rectal temperature (RT) and serum cortisol concentration were assessed before (T1), after (T2) and 60 min (T3) after the road transport. ET was evaluated with infrared thermography (IRT) in three regions of interest: EL1 (medial canthus), EL2 (central cornea) and EL3 (lateral canthus). Two-way analysis of variance (ANOVA) for repeated measures showed stati...
Bazer FW, Johnson GA.The fertilization of oocytes ovulated by pigs, sheep, cows, and horses is not considered a limiting factor in successful establishment of pregnancy. Pig, sheep, and cow embryos undergo cleavage to the blastocyst stage, hatch from the zona pellucida, and undergo central-type implantation. Hatched blastocysts of pigs, sheep, and cows transition from tubular to long filamentous forms to establish surface area for exchange of nutrients and gases with the uterus. The equine blastocyst, surrounded by external membranes, does not elongate but migrates throughout the uterine lumen before attaching to ...
Brooks J, Maeda T, Ringhofer M, Yamamoto S.The oxytocinergic system has been suggested to make up an important part of the endocrine basis of group cohesion. However, controlled studies in open-group settings have not been performed. We here investigated the impact of exogenous intranasal oxytocin on the group-level social organization of 5 groups of horses ( = 58; 12 mares and 46 geldings) through GPS tracking and social network analysis. We find oxytocin flattened social differentiation across levels. Most strikingly, oxytocin did not simply reinforce existing bonds but selectively shifted social preferences toward homogenization -...
Atabek B, Zhyldyz A, Aitakin K, Rysbek N, Jailobek O, Ahedor B, Mumbi NNM, Ma Y, Otgonsuren D, Perera WPPSI, Guswanto A, Sivakumar T, Yokoyama N.Equine piroplasmosis is caused by Theileria equi and Babesia caballi, which are hemoprotozoan parasites. Understanding the epidemiology and genotypes of T. equi and B. caballi is crucial for developing effective control strategies in endemic countries. However, the endemic status of these two parasite species remains uncertain in Kyrgyzstan due to lack of surveys. Our study, therefore, aimed to detect T. equi and B. caballi infections in Kyrgyzstan and identify their genotypes. Blood samples were collected from 226 horses across all seven provinces of Kyrgyzstan, namely Chuy, Issyk-Kul, Naryn,...
Animal welfare has become an increasingly important concern regarding equids working as carriage animals. In the present study, the changes in the markers of stress and inflammatory responses as a result of the work performed by tourism carriage horses under real working conditions in Sicily (Italy) were investigated. Twenty-two Standardbreds performed a normal working day in the carriage tourism business during the months of May, June and July 2022, consisting of one day of work for each month. Blood samples were collected in the stables at rest before the tour route (Pre; 07.00 AM) and withi...
Lale D, Dirks EE, Preining I, Lyrakis M, Gömer A, Steinmann E, Cavalleri JV, Ramsauer AS.Equine parvovirus hepatitis (EqPV-H) can cause Theiler's disease and subclinical hepatitis in horses. Objective: Assess the frequency of subclinical EqPV-H infection in hospitalized horses and to study viral transmission by investigating potential shedding routes. Methods: One hundred sixteen equids, that presented to the University Equine Hospital of the University of Veterinary Medicine Vienna between February 2021 and March 2022, for causes other than hepatopathy. Methods: In this cross-sectional study, samples (serum, feces, nasal, and buccal swabs) of hospitalized horses were collected. S...
Smirnova KP, Frill MA, Warner SE, Cheney JA.Equine back pain is prevalent among ridden horses and is often attributed to poor saddle fit. An alternative explanation is that saddle fits are technically good but fit to the wrong configuration. Saddles are fit for the standing horse, but much of the time ridden is instead spent locomoting when the back experiences the greatest peak forces. We used an array of cameras to reconstruct the surface of the back and its movement during trot, walk and standing for five horses. We verified the setup's accuracy by reconstructing a laser-scanned life-sized model horse. Our reconstructions demonstrate...
Paranjape VV, Knych HK, Berghaus LJ, Giancola S, Cathcart J, Reed RA.Anatomical location-dependent differences in transdermal opioid penetration are well described in human patients. Although this has been investigated in horses with fentanyl, there is no literature available on location-dependent plasma buprenorphine concentrations when administered as a transdermal matrix-type patch. Unassigned: This study aims to compare the plasma concentrations achieved from the matrix-type transdermal buprenorphine patches placed at different anatomical sites (metacarpus, gaskin, and ventral tail base) in healthy adult horses. Unassigned: This is a randomized experimental...
Mönki J, Mykkänen A.Mild-moderate and severe equine asthma (MEA and SEA) are prevalent inflammatory airway conditions affecting horses of numerous breeds and disciplines. Despite extensive research, detailed disease pathophysiology and the differences between MEA and SEA are still not completely understood. Bronchoalveolar lavage fluid cytology, broadly used in clinical practice and in equine asthma research, has limited means to represent the inflammatory status in the lower airways. Lipidomics is a field of science that can be utilized in investigating cellular mechanisms and cell-to-cell interactions. Studies ...
Romeiro PS, Weber SH, Justino EJ, Michelotto PV.The use of immersive methodologies is a trend in the educational environment, but their outcomes in the teaching-learning process need to be better understood. This study aimed to assess the impact of the educational environment on learning equine distal limb ultrasonography, comparing immersive classroom, traditional classroom, and virtual classroom. A total of 153 veterinary medicine students from the first to the third semester participated in this study. The students were divided into four groups: traditional (n = 22), immersive (n = 100), online (n = 31), and traditional-immer...
Nakajima K, Kasuya K, Senba H, Tagami K, Kinoshita Y, Niwa H.Strangles is a globally widespread, commonly diagnosed and important infectious disease of equids caused by Streptococcus equi subsp. equi. We performed whole genome sequencing of 19 S. equi isolates collected from imported horses at the Japanese border. Of these isolates, 15 isolates were obtained from clinical cases and 4 were from subclinical cases. The 19 isolates were grouped into 3 Bayesian analysis of population structure (BAPS) groups by the core genome single nucleotide polymorphism analysis corresponding to exporting country, SeM typing, or exporter of the horses. The 19 isolates pos...
da Luz MPF, Narciso MHPM, Maia CM, de Figueiredo Pantoja JC, Baragli P, Puoli Filho JN, de Oliveira Orsi R.We propose a practical method of assessing the personality of horses using five personality axes. Four evaluators empirically judged 19 horse individuals on specific adjectives for each axis. To validate the questionnaire, four behavioral tests were conducted with these same animals (social interactions, novel object test, bridge test,and arena test). In this tests, the frequency of specific behaviors were evaluated to create a scale related to the same personality adjectives and judge the animals based on their reactions.The questionnaire was reliable in determining the personality of horses,...
van Maanen K, van den Wollenberg L, de Haan T, Frippiat T.Monitoring infectious diseases is essential for safeguarding equine health and ensuring the sustainability of the horse industry. In 2019, the Royal Veterinary Association of the Netherlands (KNMvD) and Royal GD (GD Animal Health) launched SEIN (Surveillance of Equine Infectious diseases in the Netherlands), a voluntary surveillance system for laboratory-confirmed outbreaks of equid alphaherpesvirus 1 (EHV-1), equid alphaherpesvirus 4 (EHV-4), equine influenza virus (EIV), and subsp. . This retrospective study analyzed 364 confirmed outbreaks reported through SEIN between June 2019 and April ...
Lu Y, Ru P, Qin S, Zhang Y, Fu E, Cai M, Tuohuti N, Wu H, Zhang Y, Zhang Y.Gastrointestinal parasitic diseases pose significant health risks to equine populations. This study investigated the epidemiological patterns of equine gastrointestinal parasites in Xinjiang by analyzing 83 fecal samples collected from Ili ( = 62) and Urumqi ( = 21) between August and November 2024. The modified McMaster technique was employed to quantify fecal egg counts (EPG) and was complemented by morphological identification to assess infection dynamics related to geography, breed specificity, and management practices. The results demonstrated an overall infection prevalence of 66.3% (55/...
Doherty O, Conway R, McGreevy P.Pressures applied to horses via nosebands are of growing concern. The current study applied noseband pressure to the head of a dead horse. Pressure sensors were placed on the left nasal bone to record pressures as the noseband was progressively tightened. Tightness increased as predicated by holes in the strap of the noseband (as supplied) through eight steps from two fingers' space, assessed using the standard International Society for Equitation Science Taper Gauge through to zero space. Sensors were also placed at the midline frontal plane and intra-orally at the level of the second premola...
Maniego J, Harding C, Habershon-Butcher J, Hincks P, Stewart G, Proudman C, Ryder E.Due to the ease of collection, transport and storage, the use of dried blood spots (DBS) offers an attractive alternative matrix for detection of the abuse of gene therapy, otherwise known as gene doping. This study evaluated the recovery, extraction efficiency and resulting detection capability of DNA from DBS by evaluating different target types, DNA extraction kits, the number of punches and blood tube preservatives. The long-term storage stability of low-copy-number transgene targets in DBS was not assessed in this study but would be noteworthy to investigate further. DNA was quantified us...
Cabete A, Xufre Â, Padre L, Bettencourt E, Nunes T, Gomes J.Equine piroplasmosis (EP) is a tick-borne disease of equids caused by Theileria equi, Theileria haneyi, and Babesia caballi. EP is endemic in most tropical and subtropical regions worldwide, and there is a likelihood that it is also endemic in Portugal. This retrospective study aimed to determine the seroprevalence, prevalence, and potential risk factors of EP in our country over the past five years. A total of 3063 diagnostic test records were analysed. Results from the competitive enzyme-linked immunosorbent assay (cELISA) revealed a seroprevalence of 32.7 % and 15.7 % for T. equi and B....
Martin F, Halvarsson P, Alm YH, Tydén E.Anthelmintic resistance is an increasing problem in many gastrointestinal parasites of grazing animals. Among these, the equine roundworm, Parascaris spp., has developed wide-spread resistance to macrocyclic lactones over the past decades. Additionally, there are recent observations of emerging treatment failure of both tetrahydropyrimidine and fenbendazole. Therefore, the aims of this study were to further investigate the occurrence of fenbendazole resistance on breeding farms and to explore potential management-related risk factors associated with resistance in Parascaris spp. in Sweden. Ele...
Science (New York, N.Y.)August 28, 2025
Volume 389, Issue 6763 925-930 doi: 10.1126/science.adp4581
Liu X, Jia Y, Pan J, Zhang Y, Gong Y, Wang X, Ma Y, Alvarez N, Jiang L, Orlando L.Horsepower revolutionized human history through enhanced mobility, transport, and warfare. However, the suite of biological traits that reshaped horses during domestication remains unclear. We scanned an extensive horse genome time series for selection signatures at 266 markers associated with key traits. We detected a signature of positive selection at -known to be a modulator of behavior in mice-occurring ~5000 years ago (ya), suggesting that taming was one of the earliest steps toward domestication of horses. Intensive selection at began ~4750 ya with the domestication bottleneck, leading ...
The protein journalFebruary 22, 2025
Volume 44, Issue 3 271-282 doi: 10.1007/s10930-025-10259-8
Akay MB, Sener K, Sari S, Bodur E.Butyrylcholinesterase (BChE; EC 3.1.1.8), a serine hydrolase found in various tissues, hydrolyses choline esters such as acetylcholine and succinylcholine, as well as other esters such as heroin and acetylsalicylic acid. It is considered to play a role in lipid metabolism as it belongs to the same enzyme group as lipases and its catalytic subunits are similar. In this study, the effects of unsaturated fatty acids, namely arachidonic (AA), linoleic (LA), alpha-linolenic (ALA) and oleic acid (OA), on equine serum BChE (EqBChE) were investigated. Enzyme activity was measured by the modified Ellma...
Hébert L, Froger D, Madeline A, Lecouturier F, Lemans C, Zientara S.Dourine is a sexually transmitted parasitic disease affecting equids. Its causative agent is referred to as and the prescribed serodiagnosis method is the complement fixation test (CFT). In the context of our European Reference Laboratory mandate for equine diseases (excluding African horse sickness), we organised dourine CFT inter-laboratory proficiency tests (ILPTs) in 2015, 2018 and 2022 to evaluate the performance of the European Union network of National Reference Laboratories (NRLs) for dourine. ILPT panels were composed of horse sera with or without antibodies against spp. originating...
Arroyo-Salvo C, Cogollo Villarreal MY, Clérico G, Flores Bragulat AP, Niño Vargas A, Castañeira C, Briski O, Alonso C, Plaza J, Zeledon JM....Members of the Equus genus exhibit a fascinating capacity for hybridization, giving rise to healthy offspring. Mules, resulting from the mating of a mare with a jack, represent the most prevalent equid hybrid, serving diverse roles in our society. While in vitro embryo production, particularly through Intracytoplasmic Sperm Injection (ICSI), has rapidly gained significance in domestic horses, the in vitro production in other equids remains largely unexplored. Utilizing donkey sperm for fertilizing horse oocytes not only addresses this gap but also provides an opportunity to investigate donkey ...
Yun JU, Jung HS, Jung MJ, Song HS, Kim YB, Kim Y, Kim JG, Roh SW, Lee SH, Lee JS, Whon TW.Strain CBA3108 is a Gram-positive, non-spore-forming, obligately anaerobic bacterium isolated from horse faecal samples obtained in Jeju Island, Republic of Korea. The cells of CBA3108 are non-motile short rods that have been assessed as catalase-positive and oxidase-negative. Growth of the strain occurs under the following conditions: 25-45 °C (optimum, 35 °C); pH 6-9 (optimum, pH 6); and in the presence of 0-6 % (w/v) NaCl (optimum, 2%). Major fatty acids in the strain include C iso and C iso DMA, while major polar lipids include phosphatidylglycerol, diphosphatidylglycerol and phosp...
El Brini Z, Cullinane A, Garvey M, Fassi Fihri O, Fellahi S, Amraoui F, Loutfi C, Sebbar G, Paillot R, Piro M.This study aimed to investigate the molecular prevalence and genetic characterization of EHV-1 and EHV-4 in equid populations in Morocco. A total of 154 equids (114 horses, 9 donkeys, and 31 mules) were sampled, with nasal swabs and tissue samples subjected to multiplex real-time PCR for the detection of EHV-1 and EHV-4. Additionally, an isolate from the tissue of an aborted horse fetus was included in the analysis. A subset of EHV-positive samples underwent virus isolation followed by whole-genome sequencing. PCR assays revealed that 42 samples (27%) tested positive for EHV-4, while only 3 sa...
Khan A, Olajide E, Friedrich M, Holt A, Goehring LS.Equid alphaherpesvirus 1 (EHV-1) is a highly contagious respiratory tract pathogen of horses, and infection may be followed by myeloencephalopathy or abortion. Surveillance and early detection have focused on PCR assays using less tolerated nasal swabs. Here, we assess non-invasive non-contact sampling techniques as surveillance tools in naturally equid gammaherpesvirus 2-shedding horses as surrogates for EHV-1. Horses were individually housed for 10 h periods on 2 consecutive days. Sampling included nasal swabs, nostril wipes, environmental swabs, droplet-catching devices, and air sampling. T...
Maniego J, Harding C, Habershon-Butcher J, Hincks P, Ryder E.Gene doping, which includes the non-therapeutic use of genes or genetic elements that have the capacity to enhance athletic performance, is prohibited in horseracing and equestrian sports. To provide a comprehensive assessment of matrix and detection techniques, a custom adeno-associated virus serotype 8 vector was designed to include PCR binding sites for multiple target genes and assay types. The vector was injected via an intramuscular route into two Thoroughbred horses and matrices collected at defined timepoints. DNA was analysed using 3 detection methods: qPCR, digital PCR, and NGS. Over...
Pereira DIB, Botton SA, Ianiski LB, Braga CQ, Maciel AF, Melo LG, Zambrano CG, Bruhn FRP, Santurio JM.This systematic review compiles reports of clinical pythiosis in horses, mules and donkeys from 1960 to 2023 worldwide, focusing on Brazil. We searched databases and included 71 articles detailing clinical characteristics, geographic distribution, epidemiology, diagnostic methods, therapies, and outcomes. The results showed that publications on equine pythiosis have significantly increased since 2010. Brazil reported the highest incidence, comprising 55% of cases, predominantly in the southern, northeastern, and central-western regions during summer and autumn. Cutaneous pythiosis was the most...
Atabek B, Zhyldyz A, Aitakin K, Rysbek N, Jailobek O, Ahedor B, Mumbi NNM, Ma Y, Otgonsuren D, Perera WPPSI, Guswanto A, Sivakumar T, Yokoyama N.Equine piroplasmosis is caused by Theileria equi and Babesia caballi, which are hemoprotozoan parasites. Understanding the epidemiology and genotypes of T. equi and B. caballi is crucial for developing effective control strategies in endemic countries. However, the endemic status of these two parasite species remains uncertain in Kyrgyzstan due to lack of surveys. Our study, therefore, aimed to detect T. equi and B. caballi infections in Kyrgyzstan and identify their genotypes. Blood samples were collected from 226 horses across all seven provinces of Kyrgyzstan, namely Chuy, Issyk-Kul, Naryn,...
Gudea AI, Bârcă V, Irimie A, Martonos CO, Socaciu A.The present paper deals with the archaeozoological investigation carried out on a horse skeleton discovered in a Late Iron Age La Tène tomb (coded CX 143) in Alba Iulia, Romania. The paper presents all the results of the investigation, with a description of finds, adding a detailed assessment of the dentition with some interesting conclusions on the usage of a horse bit and the possible consequences of this use. The morphological features of the horse indicate a 7-8-year-old male individual, with a recalculated height of 1200-1300 mm. What is also stressed in the investigated sample is the la...
Pryor AJE, Ameen C, Liddiard R, Baker G, Kanne KS, Milton JA, Standish CD, Hambach B, Orlando L, Chauvey L, Schiavinato S, Calvière-Tonasso L....This paper reports a high-resolution isotopic study of medieval horse mobility, revealing their origins and in-life mobility both regionally and internationally. The animals were found in an unusual horse cemetery site found within the City of Westminster, London, England. Enamel strontium, oxygen, and carbon isotope analysis of 15 individuals provides information about likely place of birth, diet, and mobility during the first approximately 5 years of life. Results show that at least seven horses originated outside of Britain in relatively cold climates, potentially in Scandinavia or the West...
Worku A, Molla W, Kenubih A, Gizaw D, Muluneh A, Admassu B, Ejo M, Dagnaw GG, Bitew AB, Fentahun T, Getnet K, Dejene H, Berrie K, Ibrahim SM....This study aimed to estimate the prevalence, determine the distribution, and identify the epidemiological risk factors of EHV-1/-4 infections in selected districts of Northwest Amhara Region. 460 serum samples were collected from equines using multistage cluster sampling technique, and a competitive Enzyme-linked immunosorbent assay (cELISA) was performed. Various risk factors for the occurrence of EHV-1/-4 were considered. Statistical analysis was performed using R version 4.3.1. 65.9% (303) equids were tested positive for antibodies against EHV-1/-4. Based on district, the highest prevalence...
Raza A, Ijaz M, Mehmood K, Ahmed A, Javed MU, Anwaar F, Rasheed H, Ghumman NZ.Theileria equi is 1 of the emerging and prevailing tick-borne hemoprotozoans adversely affecting the equids worldwide, including Pakistan. The current study aimed to investigate the prevalence and molecular characterization of T. equi in working horses (n = 194), the comparative efficacy of different diagnostic tests, associated risk factors, and hematobiochemical analysis. The blood samples of horses were subjected to microscopic examination, cELISA, and polymerase chain reaction (PCR) and the results revealed a prevalence of 9.79, 21.13, and 13.40%, respectively, for T. equi in working horse...
Romanowski TNA, Dias RA, Heinemann MB, Carvalho SF, Silva TA, Martins ADS, Caetano GDDC, Ferreira Júnior Á, Santos JPD, Borsanelli AC.Leptospirosis, caused by Leptospira, is a zoonotic disease that, in horses, is linked to abortions, uveitis, and sporadic occurrences of liver and kidney disease, often resulting in significant economic losses for farmers. Research on the prevalence of leptospirosis in horses in the central-west region of Brazil has been relatively scarce. Thus, the present study aimed to determine the prevalence of leptospirosis in equine herds in the state of Goiás (Central Brazil). Blood samples were collected from 894 equids at 294 randomly selected farms divided into three different strata according to t...
Luciani M, Armillotta G, Di Febo T, Krasteva I, Ulisse S, Di Pancrazio C, Laguardia C, Perletta F, Serroni A, Maggetti M, Testa L, Sacchini F....The significance of as the causative agent of dourine cannot be understated, especially given its high mortality rate among equids. International movement of equids should be subject to thorough health checks and screenings to ensure that animals are not infected with . This involves the implementation of quarantine protocols, testing procedures, and the issuance of health certificates to certify the health status of the animals. Three proteins, the peptidyl-prolyl cis-trans isomerase (A0A1G4I8N3), the GrpE protein homolog (A0A1G4I464) and the transport protein particle (TRAPP) component, put...
Marcili A, Sousa IKF, Sousa RDS, Souza RA, Batista GF, Bosco VC, Santos TND, Farias BA, Miranda LM, Soares HS, Onófrio VC, Nieri-Bastos FA. Visceral leishmaniasis is a zoonosis distributed throughout Brazil and caused by the protozoan Leishmania infantum. The disease manifests in its visceral form, with dogs being the primary reservoir. Manaus is the largest city in the Brazilian Amazon region, and despite its significance, there are only documented cases of cutaneous leishmaniasis in humans. The aim of this study was to assess the occurrence of Leishmania infantum in horses in Manaus, Amazonas, Brazil. A total of 178 horses from 16 different properties within the city were sampled, and the specific molecular diagnosis was based...
Lyons ET, Drudge JH, Tolliver SC.Activity of 15 compounds, given alone or in mixtures [butamisole, cambendazole, caviphos, febantel (alone or with trichlorfon), fenbendazole, ivermectin, levamisole-piperazine, oxfendazole, oxibendazole, pyrantel pamoate (alone or with piperazine-carbon disulfide complex), thiabendazole (alone or with piperazine or with trichlorfon), tioxidazole, and trichlorfon], against Thelazia lacrymalis was evaluated in 102 equids. Determination of activity was based on comparison of infection rate in treated animals examined at necropsy with infection rate of dead equids in our contemporary surveys. None...
May-Davis S, Gee C, Brown WY.Ultrasound is a well-established modality in equine investigative and diagnostic procedures. Although to date, it has not been utilized in examining the attachments of the equine nuchal ligament lamellae (NLL) onto the cervical vertebrae, for which a noninvasive technique is currently lacking. This study presents a standardized methodology for investigating the equine NLL using ultrasonography, and further evaluates the ultrasound images against postmortem dissection in two nonrelated horses. The ultrasound images in both horses clearly presented the attachment points of the NLL to the dorsal ...
