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Topic:Equine Science
Equine Science encompasses the study of horses and their management, health, and performance. This field integrates various scientific disciplines such as biology, genetics, nutrition, physiology, and veterinary medicine to understand and improve the well-being and capabilities of horses. Areas of focus include equine anatomy, reproduction, behavior, and disease prevention. Research in equine science aims to enhance horse care, optimize training and performance, and address health challenges. This page compiles peer-reviewed research studies and scholarly articles that explore the diverse aspects of equine science, providing insights into the latest advancements and methodologies in the field.
Stallion sperm freezing with sucrose extenders: A strategy to avoid permeable cryoprotectants. The aim of this study was to assess different concentrations of sucrose-based extenders combined with bovine serum albumin (BSA) as an alternative to stallion sperm cryopreservation with permeable cryoprotectants. Semen samples (n = 16) were collected from six stallions. Sperm was cooled, filled in 0.5 mL straws and frozen in nitrogen vapor. Post-thaw sperm kinetic parameters, plasma and acrosome membrane integrity were statistically compared among treatments. In Experiment 1, extenders containing 1% of BSA and different concentrations of sucrose (mmol/L, M): 0, 50, 100, 250, 350 and 450...
A standardised equine-based welfare assessment tool used for six years in low and middle income countries. The majority of horses, donkeys and mules (equids) are in low- and middle-income countries, where they remain a key source of labour in the construction, agriculture and tourism industries, as well as supporting households daily through transporting people and staple goods. Globally, approximately 600 million people depend on working equids for their livelihood. Safeguarding the welfare of these animals is essential for them to work, as well as for the intrinsic value of the animal's quality of life. In order to manage animal welfare, it must be measured. Over the past decade, welfare assessme...
Cycle-specific female preferences for visual and non-visual cues in the horse (Equus caballus). Although female preferences are well studied in many mammals, the possible effects of the oestrous cycle are not yet sufficiently understood. Here we investigate female preferences for visual and non-visual male traits relative to the periodically cycling of sexual proceptivity (oestrus) and inactivity (dioestrus), respectively, in the polygynous horse (Equus caballus). We individually exposed mares to stallions in four experimental situations: (i) mares in oestrus and visual contact to stallions allowed, (ii) mares in oestrus, with blinds (wooden partitions preventing visual contact but allow...
How grazing affects soil quality of soils formed in the glaciated northeastern United States. Historically, much of the New England landscape was converted to pasture for grazing animals and harvesting hay. Both consumer demand for local sustainably produced food, and the number of small farms is increasing in RI, highlighting the importance of characterizing the effects livestock have on the quality of pasture soils. To assess how livestock affect pasture on Charlton and Canton soils series in RI, we examined soil quality in farms raising beef cattle (Bos taurus), sheep (Ovis aries), and horses (Equus ferus caballus), using hayed pastures as a control. We sampled three pastures per li...
Flogging tired horses: Who wants whipping and who would walk away if whipping horses were withheld? Recent studies have cast doubt on the effectiveness of whipping horses during races and this has led to questions concerning its continuing justification. Furthermore, it has been argued that whipping tired horses in racing is the most televised form of violence to animals. The present study used de-identified data from a recent independent Australian poll (n = 1,533) to characterise the 26% of respondents (113 females and 271 males) who support the whipping of racehorses and the 10% of racing enthusiasts in the sample (44 females and 63 males) who would stop watching races and betting on them...
How is the Morphometry of Stallions and Mares Show-Winning and Nonwinning Campolina Brazilian Breed with Batida and Picada Gaits? The aim of this study was to evaluate the morphometric measurements of stallion and mares show-winning and nonwinning Campolina breed with batida and picada gaits. A total of 167 horses (74 stallions and 93 mares), consisting of 102 batida and 65 picada gait animals, were used. Among these horses, 96 were awarded and 71 were not. Twenty linear, seven angular, and 11 girth measurements were taken. Results from the judging sessions were collected, and awards were observed in both sexes and both gaits. A descriptive statistical analysis and an analysis of variance were performed using the MEANS s...
Microsatellite markers for evaluating the diversity of the natural killer complex and major histocompatibility complex genomic regions in domestic horses. Genotyping microsatellite markers represents a standard, relatively easy, and inexpensive method of assessing genetic diversity of complex genomic regions in various animal species, such as the major histocompatibility complex (MHC) and/or natural killer cell receptor (NKR) genes. MHC-linked microsatellite markers have been identified and some of them were used for characterizing MHC polymorphism in various species, including horses. However, most of those were MHC class II markers, while MHC class I and III sub-regions were less well covered. No tools for studying genetic diversity of NKR com...
Effects of Equine Therapy on Individuals with Autism Spectrum Disorder: A Systematic Review. Literature on effects of equine therapy in individuals with autism spectrum disorder (ASD) has grown in recent times. Equine therapy is an alternative multimodal intervention that involves utilizing a horse to enhance core impairments in ASD. Recent systematic reviews in this area have several limitations including inclusion of populations other than ASD, assessment of a variety of animal-assisted interventions other than equine therapy, and a failure to conduct quantitative analyses to provide accurate effect size estimates. We conducted a focused systematic review to address these limitation...
Analysis of Genetic Diversity and Structure of Guanzhong Horse Using Microsatellite Markers. To determine the genetic diversity and validate the pedigree record of Chinese Guanzhong horse, 67 individuals were genotyped with eight microsatellite markers. In our study, the mean observed and expected heterozygosities were 0.51 and 0.66, respectively. The mean observed number of alleles for the Guanzhong horse was 3.88. Nonetheless, the total value of F multiloci clearly indicates that about 0.5% of overall genetic variation is due to line founder differences, while differences among individuals are responsible for the remaining 99.5%. In addition, the polymorphic information content (PIC...
Taste Preferences of Horses in Relation to Their Breed and Sex. The aim of this study was to determine the taste preferences of horses in relation to their breed and sex. Forty-eight adult purebred Arabian, Anglo-Arabian, Polish Konik, and Polish cold-blooded horses-stallions and mares in equal numbers-were examined in the study. During the experiment, five types of industrially processed feeds based on oats and natural dried products commonly regarded as horse treats (i.e., sour and sweet apples, carrots, sugar beet molasses, and barley with salt) were given to horses in buckets on three consecutive days. The behavior of the horses during the experiment ...
Stallion semen quality depends on major histocompatibility complex matching to teaser mare. The major histocompatibility complex (MHC) has repeatedly been found to influence mate choice of vertebrates, with MHC-dissimilar mates typically being preferred over MHC-similar mates. We used horses (Equus caballus) to test whether MHC matching also affects male investment into ejaculates after short exposure to a female. Semen characteristics varied much among stallions. Controlling for this variance with a full-factorial within-subject experimental design, we found that a short exposure to an MHC-dissimilar mare enhanced male plasma testosterone and led to ejaculates with elevated sperm nu...
Pregnancy Rates Following Low-Temperature Storage of Large Equine Embryos Before Vitrification. Satisfactory pregnancy rates can now be achieved following the cryopreservation of large equine embryos. Nonetheless, its wide application might be limited by the fact that the cryopreservation of large equine embryos requires a specialized micromanipulation equipment and micromanipulation/vitrification skills. Alternatives should be developed to increase its utilization and widespread application in the commercial equine industry. To determine if large equine embryos are able to remain viable during transport from farms to specialized centers for embryo cryopreservation, we evaluated pregnanc...
Subchondral bone morphology in the metacarpus of racehorses in training changes with distance from the articular surface but not with age. The repetitive large loads generated during high-speed training and racing commonly cause subchondral bone injuries in the metacarpal condyles of racehorses. Adaptive bone modelling leads to focal sclerosis at the site of highest loading in the palmar aspect of the metacarpal condyles. Information on whether and how adaptive modelling of subchondral bone changes during the career of a racehorse is sparse. The aim of this cross-sectional study was to describe the changes in subchondral bone micromorphology in the area of highest loading in the palmar aspect of the metacarpal condyle in thorough...
Improved cryosurvival of stallion spermatozoa after colloid centrifugation is independent of the addition of seminal plasma. Addition of seminal plasma (SP) prior to cryopreservation may influence stallion sperm cryosurvival. The objective of this study was to investigate the addition of pooled SP from "good" or "bad" freezer stallions to spermatozoa selected by single layer centrifugation (SLC) prior to cryopreservation on post-thaw sperm quality. Semen from 12 stallions was collected; 5 mL was frozen as control (C) and the remainder was processed by SLC to remove SP and was divided into three aliquots: i) SLC sample without SP (SLC); ii) SLC plus pooled SP from "good freezer" stallions (SLC-GF); iii) SLC plus po...
Cultured equine satellite cells as a model system to assess leucine stimulated protein synthesis in horse muscle. Leucine has been shown to stimulate the mammalian/mechanistic target of rapamycin (mTOR) signaling pathway which plays numerous key regulatory roles in cell growth, survival, and metabolism including protein synthesis in a number of species. However, previous work with equine satellite cells has suggested distinct species differences in regards to physiological effects and the magnitude of responses to growth factors and regulators. Because there is limited research available regarding the role of leucine in regulating equine skeletal muscle protein synthesis, the objective of this study was t...
Variations during ageing in the three-dimensional anatomical arrangement of fascicles within the equine superficial digital flexor tendon. BiTendons are constructed from collagenous fascicles separated by endotenon/interfascicular matrix (IFM). Tendons may be specialised for precision movement or to store energy during locomotion and for the latter the elasticity of the endotenon/IFM is particularly important. The equine superficial digital flexor tendon (SDFT) is a dedicated energy-storing tendon with a similar function to the human Achilles tendon. Classical anatomical descriptions portray fascicles as longitudinally arranged distinct anatomical structures. In the present study, using three-dimensional reconstruction from whole...
Equine Caregiver Information-Seeking Preferences: Surveys in the Midwest. Two surveys of equine owners/managers and professionals using convenience sampling via multimodal distribution were conducted on perceptions of equid health and well-being (n = 142) and equine nutrition and feeding practices (n = 151). Surveys were distributed in 2014-2015 (health and well-being) and 2016 (nutrition and feeding) to similar email lists and social media sites; both included questions regarding information-seeking preferences. Respondents were mostly female (62% health and well-being, 84% nutrition and feeding) and had over 20 years of equine ownership/management experience (4...
Lactoferrin increases sperm membrane functionality of frozen equine semen. During cryopreservation, sperm was submitted to an increase in reactive oxygen species generation. This work aimed to improve the quality of frozen equine sperm after the addition of antioxidants lactoferrin (Lf) and catalase (Cat) to a freezing extender. Semen from six stallions was frozen with the extenders: F1) control, INRA 82 freezing extender, F2) F1 + 500 μg/ml Lf and F3) F1 + 200 IU/ml Cat. After thawing, sperm motility parameters, membrane functionality and integrity, and acrosome integrity and spontaneous acrosome-reacted sperm were evaluated with a computer-assisted sperm anal...
Detection of prohibited substances in equine hair by ultra-high performance liquid chromatography-triple quadrupole mass spectrometry – application to doping control samples. The detection of drugs in human hair samples has been performed by laboratories around the world for many years and the matrix is popular in disciplines, such as workplace drug testing. To date, however, hair has not become a routinely utilised matrix in sports drug detection. The analysis of hair samples offers several potential advantages to doping control laboratories, not least of which are the greatly extended detection window and the ease of sample collection and storage. This article describes the development, validation, and utilisation of a sensitive ultra-high performance liquid chro...
Probiotic Use in Equine Gastrointestinal Disease. Probiotics are commonly used in human and veterinary medicine due to their postulated positive effects on overall and specifically gastrointestinal health. Although some beneficial effects have been shown in several human diseases, a general beneficial effect of probiotics is currently not supported. In horses, well-designed studies to date are few, results are conflicting, and the effects of probiotics are questionable. Adverse effects are rare; however, intestinal adverse effects (diarrhea) have been reported in foals. Quality control of over-the-counter probiotics is not tightly regulated, ...
Understanding the Intestinal Microbiome in Health and Disease. This article provides readers with the basic concepts necessary to understand studies using recent molecular methods performed in intestinal microbiome assessment, with special emphasis on the high throughput sequencing. This review also summarizes the current knowledge on this topic and discusses future insights on the interaction between the intestinal microbiome and equine health.
Palmar annular ligament desmotomy in horses with the Arthrex-Centerline™ : An ex-vivo study. The aim of this study was to evaluate the use of a single-portal endoscopic desmotomy of the PAL with the use of Arthrex - Centerline™. Ten horse distal front limbs from horses free of PAL disease were prepared for tenoscopy of the digital flexor tendon sheath (DFTS). A dual-port endoscopic desmotomy with a hook knife was performed in 5 specimens (Group A) and single port Arthrex-Centerline™ Desmotomy was performed in another 5 specimens (Group B). The performing time, judgments of the surgeon, number of blade passages, and gross anatomy were evaluated. The performing time and surgeon judg...
Determinants of gestation length in Thoroughbred mares on German stud farms. The aim of the present study was to analyze the effects of stallion and mare, their ages, and maternal lineage on the gestation length (GL) in Thoroughbreds. In addition, additive genetic effects of the dam, stallion and fetus were analyzed. Data were taken from 1993 through 2009, and included 16,226 pregnancies from 5959 Th oroughbred mares mated with 290 different stallions. All analyses were performed using linear mixed models. The GL ranged from 306 to 390 days, with a mean length of 347.0 ± 14.4 days. Mating of mares with stallions aged 17 years and older resulted in a significantly ...
Morphofunctional diversity of equine of varied genetic compositions raised in the Pantanal biome of Brazil. Evaluating phenotypic diversity makes it possible to identify discrepancies in aptitudes among animals of different genetic bases, which is an indicator of adaptive or selective differences between populations. The objective of this work was to evaluate the morphofunctional diversity of 452 male and female adult equines (Arabian, Quarter Mile, Pantaneiro, and Criollo breeds, and undefined crossbreeds of horses and mules) raised in the Pantanal biome (Brazil). Linear measurements were performed to estimate conformation indexes. Initially, a discriminant analysis was performed, regardless of the...
New Perspectives in Equine Intestinal Parasitic Disease: Insights in Monitoring Helminth Infections. Regular anthelmintic treatment has contributed to anthelmintic resistance in horse helminths. This mass anthelmintic treatment was originally developed owing to a lack of larvicidal drugs against Strongylus vulgaris. The high prevalence of anthelmintic resistance and shortening of strongyle egg reappearance period after avermectins/moxidectins requires epidemiologically appropriate and sustainable measures. Selective anthelmintic treatment is a much-needed deworming approach: More than 50% of adult horses manifest no strongyle egg excretion. In this article, selective anthelmintic treatment pr...
Improving working equine welfare in ‘hard-win’ situations, where gains are difficult, expensive or marginal. Brooke is a non-government organisation with working equine welfare programmes across Africa, Asia and Latin America. In 2014, staff from ten country programmes were asked to identify 'no-win' situations (subsequently reframed as 'hard-wins')-where improving equine welfare is proving difficult, expensive and/or marginal-in order to inform strategic decisions on how to approach, manage and mitigate for such situations. The Delphi-type consultation process had three phases. Round 1 posed five questions in the form of a workshop, survey and semi-structured interviews. Round 2 re-presented key the...
Short-term tolerance of equine spermatozoa to various abiotic factors. The aim of this study was to determine the effects of various abiotic factors, such as light, physical stress (pipetting) and thermal shock, on the quality of fresh and cooled equine sperm. In experiment I, four sperm aliquots were subjected to different light exposures: (i) protected control samples (CTRL), (ii) exposed to UV light at 10 cm (UV10), (iii) exposed to UV light at 20 cm (UV20) and (iv) exposed to laboratory lighting (LAB). In experiment II, four semen aliquots were subjected to repeated pipetting for 0, 10, 20 and 30 times (CTRL, P10, P20 and P30, respectively). In experiment I...
Stress level effects on sport performance during trotting races in Spanish Trotter Horses. The stress level is suggested to have a negative effect on horses during equestrian competitions, specifically in trotter racing. The main objectives of this study were to measure stress levels in Spanish Trotter Horse races with a reliable non-invasive method, and determining the threshold level of stress that leads to the best performance results and also shows when the stress perceived by the horse becomes distress. One hundred and thirty individuals were evaluated, measuring their performance (based on racing time per kilometre (TPK)) and their stress (based on eye temperature, assessed wi...
