Analyze Diet

Topic:Feed Intake

Feed intake in horses refers to the amount and type of feed consumed by horses, which can vary based on factors such as age, activity level, health status, and environmental conditions. It is a critical aspect of equine management, influencing growth, performance, reproduction, and overall health. Feed intake can be affected by the nutritional content of the diet, palatability, feeding practices, and physiological needs of the horse. Understanding feed intake patterns and requirements is essential for formulating appropriate feeding strategies. This page compiles peer-reviewed research studies and scholarly articles that explore the determinants of feed intake, its impact on equine physiology, and strategies for optimizing feeding practices in horses.
Renal energy excretion of horses depends on renal hippuric acid and nitrogen excretion.
Journal of animal physiology and animal nutrition    June 13, 2017   Volume 102, Issue 1 e380-e386 doi: 10.1111/jpn.12756
Hipp B, Südekum KH, Zeyner A, Goren G, Kienzle E.The prediction of renal energy excretion is crucial in a metabolizable energy system for horses. Phenolic acids from forage cell walls may affect renal energy losses by increasing hippuric acid excretion. Therefore, the relationships were investigated between renal energy, nitrogen (N) and hippuric acid excretion of four adult ponies (230-384 kg body weight (BW)) consuming diets based on fresh grass, grass silage, grass cobs (heat-dried, finely chopped, pressed grass), alfalfa hay, straw, extruded straw and soybean meal. Feed intake was measured; urine and faeces were quantitatively collected...
Livestock metabolomics and the livestock metabolome: A systematic review.
PloS one    May 22, 2017   Volume 12, Issue 5 e0177675 doi: 10.1371/journal.pone.0177675
Goldansaz SA, Guo AC, Sajed T, Steele MA, Plastow GS, Wishart DS.Metabolomics uses advanced analytical chemistry techniques to comprehensively measure large numbers of small molecule metabolites in cells, tissues and biofluids. The ability to rapidly detect and quantify hundreds or even thousands of metabolites within a single sample is helping scientists paint a far more complete picture of system-wide metabolism and biology. Metabolomics is also allowing researchers to focus on measuring the end-products of complex, hard-to-decipher genetic, epigenetic and environmental interactions. As a result, metabolomics has become an increasingly popular "omics" app...
Effects of training distance on feed intake, growth, body condition and muscle glycogen content in young Standardbred horses fed a forage-only diet.
Animal : an international journal of animal bioscience    April 3, 2017   Volume 11, Issue 10 1718-1726 doi: 10.1017/S1751731117000593
Ringmark S, Revold T, Jansson A.This study examined feed intake, growth, body condition, muscle glycogen content and nutrition-related health in 16 Standardbred horses fed a high-energy, forage-only diet ad libitum and allocated to either a control training programme (C-group) or a training programme with the high-intensity training distance reduced by 30% (R-group), from January as 2-year olds until December as 3-year olds. Feed intake was recorded on 10 occasions during 3 consecutive days. Body weight was recorded once in a week and height, body condition score (BCS), rump fat thickness and thickness of the m. longissimus ...
Comparative fibre-degrading capacity in foals at immediate and late post-weaning periods.
Animal : an international journal of animal bioscience    February 21, 2017   Volume 11, Issue 9 1497-1504 doi: 10.1017/S1751731117000349
Faubladier C, Julliand V, Beuneiche L, Philippeau C.Total tract apparent digestibility of dietary fibrous components and parameters of the faecal digestive ecosystem were compared at immediate and late post-weaning periods using five foals. The foals were abruptly weaned (day 0) from their mares at 6 months of age. Immediately (day 0 to day 14) and later (day 131 to day 194) after weaning, foals received the same basal diet consisting of hay and pellets. Pellets were fed at 0.36% BW. Hay was offered ad libitum and corresponded to 120% of the average voluntary hay intake measured individually during the immediate (days 1 to 4) and late (days 180...
Effects of forage species and poultry litter application timing on forage preference by horses.
Journal of animal science    January 4, 2017   Volume 94, Issue 12 4985-4992 doi: 10.2527/jas.2016-0564
Clark JK, Shanks BC, Jogan KS, Philipp D, Coffey KP, Jack NE, Caldwell JD, Rhein RT.Bermudagrass ( L.) is a familiar forage in the equine industry and teff () is gaining popularity as well. However, it is unclear if the application of poultry litter as a fertilizer affects palatability of these forages in horses. Therefore, the objective of this study was to determine if forage species and timing of litter application as a fertilizer has an effect on preference by horses. Hay treatments were arranged in a 2 × 3 factorial treatment arrangement consisting of teff and bermudagrass harvested after no poultry litter application (NL), poultry litter applied to stubble immediately ...
Mouldy feed: A possible explanation for the excretion of anabolic-androgenic steroids in horses.
Drug testing and analysis    July 23, 2016   Volume 8, Issue 5-6 525-534 doi: 10.1002/dta.2023
Decloedt AI, Bailly-Chouriberry L, Vanden Bussche J, Garcia P, Popot MA, Bonnaire Y, Vanhaecke L.To ensure fair competition and to protect the horse's welfare, horses have to compete on their own merits, without any unfair advantage that might follow the use of drugs. Therefore, regulatory authorities list all substances that are not allowed in competition, including most anabolic-androgenic steroids. As zero-tolerance is retained, the question arose whether the consumption of mouldy feed could lead to the excretion of steroids, due to the biotransformation of plant phytosterols to steroids. A rapid ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) analyt...
Incorporation of sunflower oil or linseed oil in equine compound feedstuff: 1 Effects on haematology and on fatty acids profiles in the red blood cells membranes.
Journal of animal physiology and animal nutrition    April 14, 2016   Volume 100, Issue 5 828-835 doi: 10.1111/jpn.12448
Patoux S, Istasse L.Eight trained horses (6 mares - 2 geldings, 6 Selle Français, 2 Trotteur Français, 12 ± 5.8 years old, 538 ± 72.5 kg) were offered three diets to potentially affect haematology and the fatty acids (FA) profiles in red blood cells (RBC) membranes. The control diet was composed of 50% hay and 50% concentrate containing mainly rolled barley (48%) and whole spelt (48%). In the case of sunflower oil diet, sunflower oil (62.0% of α-linoleic acid, LA) was incorporated at a rate of 8% and substituted by an equal proportion of barley. In the linseed oil diet, first cold-pressed linseed oil (...
Feed, supplements and contamination risks under FEI Rules: Insight from the Guerdat and Bichsel decisions.
Equine veterinary journal    January 29, 2016   Volume 48, Issue 2 135-137 doi: 10.1111/evj.12549
Pheasant J.No abstract available
Effects of deoxynivalenol in naturally contaminated wheat on feed intake and health status of horses.
Mycotoxin research    September 30, 2015   Volume 31, Issue 4 209-216 doi: 10.1007/s12550-015-0234-6
Schulz AK, Kersten S, Dänicke S, Coenen M, Vervuert I.The present study examined the short-term effects of deoxynivalenol (DON), administered at two different concentrations via a feed preparation using naturally contaminated wheat, on feed intake, liver and kidney metabolism and immunomodulatory properties in horses. Twelve geldings were randomly assigned to one of three dietary treatments for 21 days. DON was provided via naturally contaminated wheat (14.6 ± 6.5 mg DON/kg dry matter). The daily feed intake was adjusted to 4 kg of wheat and 1.7 kg of silage per 100 kg of body weight (BW). Horses were fed one of the following diets: control whea...
Daily methane production pattern of Welsh ponies fed a roughage diet with or without a cereal mixture.
Journal of animal science    May 29, 2015   Volume 93, Issue 4 1916-1922 doi: 10.2527/jas.2014-8415
Dansen O, Pellikaan WF, Hendriks WH, Dijkstra J, Jacobs MP, Everts H, van Doorn DA.Methane production from Welsh ponies fed 2 isoenergetic diets (NE basis) at maintenance was studied in a crossover design with 4 mature geldings (230 ± 10.5 kg BW, mean ± SE). Treatments included a roughage-only (R) diet (5.1 kg DM/d) or a roughage plus cereal mix (RC) diet (2.5 kg DM hay/d plus 1.1 kg DM cereal mix/d). For both diets, the same grass hay was used (898 g DM/kg and 4.5 MJ NE/kg DM) and a commercial cereal mix was used in the RC diet (890 g DM/kg and 9.6 MJ NE/kg DM). Ponies were housed in pairs in climate-controlled respiration chambers. Carbon dioxide production (CO2), oxygen...
Energy supplementation and herbage allowance effects on daily intake in lactating mares.
Journal of animal science    May 29, 2015   Volume 93, Issue 5 2520-2529 doi: 10.2527/jas.2014-8447
Collas C, Dumont B, Delagarde R, Martin-Rosset W, Fleurance G.Little is known about how to manage grazing horses, including the thresholds under which energy supplementation is required. Here we investigated the effects of daily herbage allowance (DHA) and energy supplementation (ES) on daily herbage intake in lactating mares of light breeds grazing high-quality regrowth during summer. Three contrasting DHA, low (LOW), medium (MED), and high (HIGH), that is, 35.0, 52.5, and 70.0 g DM∙kg BW(-1)∙d(-1), respectively, were obtained by adjusting pasture strip width. Eighteen Anglo-Arab and French Saddle lactating mares were either supplemented with 2.6 kg...
[Alimentary intake of opioid alkaloids by horses. Hazards due to poppy-containing feeds].
Tierarztliche Praxis. Ausgabe G, Grosstiere/Nutztiere    January 27, 2015   Volume 43, Issue 1 35-43 doi: 10.15653/TPG-140638
Hertzsch R, Emmerich IU, Lachenmeier DW, Sproll C, Monakhova YB, Aboling S, Bachmann U, Vervuert I.Opioid alkaloids were identified in the urine of horses during an anti-doping control and in a case of intoxication. In both cases, it was suspected that the horses had ingested poppy-contaminated feed. To verify this suspicion, possible opioid alkaloid sources in Germany were identified through a literature research. Additionally, the contaminated feed was botanically and chemically analysed. The results indicated that both cases were most probably caused by the poppy in the feed. This highlights the previously underestimated risk of an intake of poppy-contaminated feed in horses. Recommendat...
In vivo performance of Italian Heavy Draft Horse weanlings fed two protein levels and slaughtered at two ages.
Journal of animal science    October 29, 2014   Volume 92, Issue 11 4998-5008 doi: 10.2527/jas.2014-7875
Mantovani R, Guzzo N, Sartori C, Bailoni L.This study aimed at evaluating in vivo performance, growth parameters, intakes, dressing percentage, and blood parameters in Italian Heavy Draft Horse (IHDH) weanlings fed 2 CP levels up to the 2 typical ages of slaughter. Forty-one weanlings were grouped in 8 pens according to sex, age, and BW. After a transition period, animals were randomly assigned to 2 isoenergetic diets containing different CP levels: 10.6 and 11.2% CP in DM for low protein (LP) and 13.2 and 14.7% CP in DM for high protein (HP) diets in the first and second phase, respectively. About half of the animals (n = 22) were sla...
[Energy intake and body weight development of Warmblood foals that changed stud at weaning].
Tierarztliche Praxis. Ausgabe G, Grosstiere/Nutztiere    August 15, 2014   Volume 42, Issue 4 220-224 
Mack JK, Remler HP, Senckenberg E, Kienzle E.This study investigated the energy requirements of Warmblood foals with a change of the stud at weaning. Methods: Nine colts purchased at weaning participated in the study aged approximately 6 months to 1 year. They were transported to the stud by their breeders either having been separated from their dams in their home stable or upon arrival at the stud. The foals were offered a late first cut of haylage, oats and foal starter feed. To ensure individual feeding of concentrates, the foals were tethered twice daily. The total combined haylage intake of all foals per day was recorded. Individu...
The influence of nutrition on the insulin-like growth factor system and the concentrations of growth hormone, glucose, insulin, gonadotropins and progesterone in ovarian follicular fluid and plasma from adult female horses (Equus caballus).
Reproductive biology and endocrinology : RB&E    July 31, 2014   Volume 12 72 doi: 10.1186/1477-7827-12-72
Salazar-Ortiz J, Monget P, Guillaume D.Feed intake affects the GH-IGF system and may be a key factor in determining the ovarian follicular growth rate. In fat mares, the plasma IGF-1 concentration is high with low GH and a quick follicular growth rate, in contrast to values observed in thin mares. Nothing is known regarding the long-term effects of differential feed intake on the IGF system. The objective of this experiment was to quantify IGFs, IGFBPs, GH, glucose, insulin, gonadotropin and progesterone (P4) in blood and in preovulatory follicular fluid (FF) in relation to feeding levels in mares. Methods: Three years prior to the...
Nitrogen digestion and urea recycling in Hokkaido native horses fed hay-based diets.
Animal science journal = Nihon chikusan Gakkaiho    July 12, 2014   Volume 86, Issue 2 159-165 doi: 10.1111/asj.12256
Obitsu T, Hata H, Taniguchi K.Nitrogen (N) digestion and urea-N metabolism in Hokkaido native horses fed roughage-based diets containing different types and levels of protein sources were studied. Horses (173 ± 4.8 kg) fitted with an ileum cannula were fed four diets consisting of 100% timothy hay (TH), 88% TH and 12% soybean meal (SBM), 79% TH and 21% SBM, and 51% TH and 49% alfalfa hay at 2.2% of body weight. Dietary protein content varied from 5% to 15% of dry matter. Apparent N digestibilities in the pre-cecum and total tract for the TH diet were lower than those for other diets. However, the proportion of post-ileum ...
Effect of pectin, lecithin, and antacid feed supplements (Egusin®) on gastric ulcer scores, gastric fluid pH and blood gas values in horses.
BMC veterinary research    July 7, 2014   Volume 10 Suppl 1, Issue Suppl 1 S4 doi: 10.1186/1746-6148-10-S1-S4
Woodward MC, Huff NK, Garza F, Keowen ML, Kearney MT, Andrews FM.The objectives of this study were to evaluate the effects of two commercial feed supplements, Egusin 250® [E-250] and Egusin SLH® [E-SLH], on gastric ulcer scores, gastric fluid pH, and blood gas values in stall-confined horses undergoing feed-deprivation. Methods: Nine Thoroughbred horses were used in a three-period crossover study. For the three treatment groups, sweet feed was mixed with E-250, E-SLH, or nothing (control group) and fed twice daily. Horses were treated for 21 days, then an additional 7 days while on an alternating feed-deprivation model to induce or worsen ulcers (period o...
How does the suppression of energy supplementation affect herbage intake, performance and parasitism in lactating saddle mares?
Animal : an international journal of animal bioscience    June 2, 2014   Volume 8, Issue 8 1290-1297 doi: 10.1017/S175173111400127X
Collas C, Fleurance G, Cabaret J, Martin-Rosset W, Wimel L, Cortet J, Dumont B.Agroecology opens up new perspectives for the design of sustainable farming systems by using the stimulation of natural processes to reduce the inputs needed for production. In horse farming systems, the challenge is to maximize the proportion of forages in the diet, and to develop alternatives to synthetic chemical drugs for controlling gastrointestinal nematodes. Lactating saddle mares, with high nutritional requirements, are commonly supplemented with concentrates at pasture, although the influence of energy supplementation on voluntary intake, performance and immune response against parasi...
Effect of grass hay intake on fiber digestion and digesta retention time in the hindgut of horses.
Journal of animal science    March 18, 2014   Volume 92, Issue 4 1574-1581 doi: 10.2527/jas.2013-6676
Miyaji M, Ueda K, Hata H, Kondo S.Eight Thoroughbred horses were used to examine the effects of grass hay intake on the fiber digestion and the retention time of digesta in the total gastrointestinal tract and the hindgut segments. The horses were randomly assigned to 2 groups and offered 2.0 (high intake [HI]) or 1.3 kg DM/(100 kg BW • d; low intake [LI]) of timothy hay in equal amounts every 3 h for 17 d. The digestibility and total tract mean retention time of digesta (tMRT) in the total gastrointestinal tract were measured from d 11 to d 15. To measure the mean retention time of digesta in each hindgut segment (sMRT), th...
No effect of moderate or high concentrate allowance on growth parameters in weanling Warmblood foals fed late-cut haylage as forage.
Journal of animal physiology and animal nutrition    January 15, 2014   Volume 98, Issue 5 886-893 doi: 10.1111/jpn.12153
Mack JK, Remler HP, Senckenberg E, Kienzle E.Two groups of Warmblood foals from the Bavarian federal stud participated in the study beginning from the age of approximately 6 months. The foals were offered a late 1st cut of haylage, oats and foal starter feed. For 2 months after weaning, group 'R' (15 foals) received an amount of oats to provide a total digestible energy supply meeting the recommendations of the German Society of Nutrition Physiology (GfE), whereas the other group 'A' (16 foals) was offered a higher amount of oats (surplus of approximately 1.3 kg/animal/day). Concentrates were fed individually twice daily; total daily ...
[Diarrhoea and oedema in two show horses after feeding a pelleted supplemental feed for horses according to VDLUFA’s perspective of microbial quality classified as safe for use in horses].
Berliner und Munchener tierarztliche Wochenschrift    August 2, 2013   Volume 126, Issue 7-8 342-349 
Büsing K, Mietke-Hofmann H, Dibbert R, Donandt D, Maier T, Zeyner A.A new batch of a supplemental feed was fed as pellets (diameter 8 mm) to two Warmblood-type horses. One horse developed watery diarrhoea within two days. Pronounced oedema due to hypalbuminemia was seen about ten days later. The feed was replaced by pellets of identical composition and mixing process, but lower diameter (5 mm). After one week of feeding, oedema regressed and faeces were normally formed. At refeeding the larger sized pellets, the symptoms recurred, but now both horses were affected. After a change to the smaller pellets, the horses recovered soon. Water activity (aw-value) of t...
Influence of maternal plane of nutrition on mares and their foals: determination of mare performance and voluntary dry matter intake during late pregnancy using a dual-marker system.
Journal of animal science    July 3, 2013   Volume 91, Issue 9 4208-4215 doi: 10.2527/jas.2013-6373
Winsco KN, Coverdale JA, Wickersham TA, Lucia JL, Hammer CJ.Thirty pregnant mares (538 to 695 kg BW; 4 to 19 yr of age) were used to evaluate the effects of plane of nutrition on DMI of hay and mare performance (BW, BCS, and rump fat) during the last third of pregnancy. Mares were divided into 4 blocks by their expected foaling date and randomly assigned within block to either a hay or concentrate plus hay diet (concentrate fed at 0.75% BW, as-fed basis) with 15 mares per treatment. Treatments began 110 d before expected foaling date (230 d of gestation) and terminated at parturition. Mares were housed by block and allowed ad libitum access to coastal ...
Eating disorders and equine therapy: a nurse’s perspective on connecting through the recovery process.
Journal of psychosocial nursing and mental health services    June 21, 2013   Volume 51, Issue 9 24-31 doi: 10.3928/02793695-20130612-01
Dezutti JE.Patients with eating disorders may have the most complex interdisciplinary treatment plans of any mental illness. Nurses need innovative evidence-based treatment interventions to assist their patients with eating disorders on their road to recovery. Although much has been written about equine-assisted psychotherapy (EAP) and equine-facilitated psychotherapy, the literature has not described a detailed session that can help nurses understand how this experiential treatment works and the impact it can have on the patient. A review of the literature on eating disorders and on the use of equine th...
Seasonal changes of total body water and water intake in Shetland ponies measured by an isotope dilution technique.
Journal of animal science    June 4, 2013   Volume 91, Issue 8 3750-3758 doi: 10.2527/jas.2012-5317
Brinkmann L, Gerken M, Riek A.Water is an essential nutrient necessary to support life, and adequate water supply is crucial for animal survival and productivity. The present study was designed to determine seasonal changes in the water metabolism of horses under outdoor conditions. Total body water (TBW) and total water intake (TWI) of 10 adult Shetland pony mares were estimated at monthly intervals for 14 mo by using the deuterium dilution technique. During the last 4 mo, 5 ponies were fed restrictively to simulate natural feed shortage in winter, and 5 ponies served as controls. The TBW (kg) was closely related to body ...
Determination of Zn-, Cu- and Mn-glycinate complexes in feed samples and in-vitro and in-vivo assays to assess their bioaccessibility in feed samples.
Talanta    April 6, 2013   Volume 113 14-18 doi: 10.1016/j.talanta.2013.03.083
Vacchina V, Ionescu C, Oguey S, Lobinski R.A method was developed for the quantification of Zn-, Cu- and Mn-glycinates in supplemented feed samples. The coupling of capillary electrophoresis (CE) with ICP MS detection after purification of the extract by ultrafiltration was shown to be efficient for the quantitative recovery of glycinates. The method developed was then applied to evaluate the bioaccessibility of glycinates using a sequential enzymolysis approach. The data obtained indicated a strong bioaccessibility of each element (79-94%). A new complex was also found to be formed during the digestion process. Bioavailability was the...
Effect of long-term feed restriction on the health status and welfare of a robust horse breed, the Shetland pony (Equus ferus caballus).
Research in veterinary science    November 8, 2012   Volume 94, Issue 3 826-831 doi: 10.1016/j.rvsc.2012.10.010
Brinkmann L, Gerken M, Riek A.Outdoor group housing is increasingly recognized as an appropriate housing system for domesticated horses. The objective of this study was therefore to investigate the effect of potential feed shortage in semi-natural horse keeping systems in winter on animal health and welfare. In 10 female Shetland ponies blood concentrations (NEFA, total protein (TP), total bilirubin (TB), beta-hydroxybutyrate (BHB) and thyroxine (T4)), body mass and the body condition score (BCS) were monitored for 7 months including a 4 months period of feed restriction in five of the 10 ponies. Restrictively fed animals ...
Rapid method for the simultaneous determination of six ionophores in feed by liquid chromatography/mass spectrometry.
Journal of AOAC International    September 14, 2012   Volume 95, Issue 4 1016-1022 doi: 10.5740/jaoacint.11-023
Vudathala D, Murphy L.A simple and highly sensitive LC/MS method was developed for the simultaneous determination of six ionophores--lasalocid, monensin, laidlomycin, maduramycin, salinomycin, and narasin--in feed. The procedure involved extraction of 1 g of feed with 4 mL of methanol-water (9 + 1, v/v) by shaking on a platform shaker for 45 min. After centrifugation, the extracts were diluted with methanol-water (75 + 25, v/v) and analyzed without any cleanup. The analysis was performed on a Betasil C18 column (150 x 4.6 mm id, 5 pm particle size) connected to an LC/MS system operated in the atmospheric pressure c...
Feed restriction enhances the depressive effects of erythromycin on equine hindgut microbial metabolism in vitro.
Berliner und Munchener tierarztliche Wochenschrift    August 28, 2012   Volume 125, Issue 7-8 351-358 
Kuhn M, Guschlbauer M, Feige K, Schluesener M, Bester K, Beyerbach M, Breves G.Equine typholocolitis is a sporadic diarrheal disease causing high mortality rates. One of the risk factors responsible for this is the oral application of the macrolide antibiotic erythromycin. The aim of the present in vitro study was to investigate whether erythromycin in combination with feed restriction provokes changes in microbial hindgut metabolism and could therefore be involved in the pathogenesis of equine typhlocolitis. As application of erythromycin and feed restriction are risk factors for equine typhlocolitis, both factors were chosen to investigate their individual and combined...
[Influence of the amount of concentrate feeding on concentrate intake and development of body weight and growth parameters of suckling foals from birth until the 6th month of life].
Tierarztliche Praxis. Ausgabe G, Grosstiere/Nutztiere    June 13, 2012   Volume 40, Issue 3 150-156 
Mack JK, Remler HP, Senckenberg E, Kienzle E.The objective of this study was to investigate the effects of a different energy supply on the development of Warmblood foals with a focus on examining the recommended allowances of the German Society for Nutrition Physiology. Methods: Two groups of foals received different amounts of concentrates from the 1st until the 6th month of life. With regards to the total energy content, the rations were composed to either comply with the recommendations (6) (group "Norm", n=15) or to exceed those by approximately 20% (group "Zulage", n=16). The supply with concentrates of the group "Norm" aimed for a...
Voluntary feed intake and leptin sensitivity in ad libitum fed obese ponies following a period of restricted feeding: a pilot study.
Journal of animal physiology and animal nutrition    April 5, 2012   Volume 97, Issue 4 624-631 doi: 10.1111/j.1439-0396.2012.01300.x
Van Weyenberg S, Buyse J, Kalmar ID, Swennen Q, Janssens GP.The relation between plasma leptin and daily ad libitum roughage intake was evaluated during a 14-day period in eight obese Shetland ponies. When the feeding strategy was changed from maintenance feeding to ad libitum feeding, feed intake increased the first day, decreased the second day and increased again during the following days to reach a constant level after 8 days. Plasma leptin concentration increased during the first 2 days, but remained constant afterwards. Although the same pattern was found in all ponies, the magnitude of the increase in leptin on day 1 and the resulting decrease i...