Analyze Diet

Topic:Forage

Forage is a primary component of the equine diet, consisting mainly of grasses, hay, and legumes that provide essential nutrients and fiber. It plays a significant role in maintaining digestive health and supporting the overall well-being of horses. The nutritional composition of forage can vary based on factors such as plant species, maturity at harvest, and environmental conditions. Proper management and selection of forage are important for meeting the dietary needs of horses and preventing issues such as colic or laminitis. This page compiles peer-reviewed research studies and scholarly articles that explore the nutritional value, management practices, and impact of forage on equine health and performance.
The effects of diet on the physical characteristics of feces and fiber digestibility in healthy horses.
Journal of animal science    August 13, 2026   skag255 doi: 10.1093/jas/skag255
Starrett A, Sarturi JO, Leatherwood JL, Chaffin MK, Mukhopadhyay S, Pilla R, Arnold CE.Diet is a known risk factor for colic, but few studies have explored how diet affects fecal characteristics which are often altered in horses with colic. This prospective cross-over study evaluated the effects of two common equine diets on fecal characteristics and fiber digestibility in healthy adult horses. We hypothesized that horses offered a complete pelleted diet would exhibit increased fecal dry matter (DM) and decreased fecal particle size compared to those offered a forage-based diet. Twenty horses at Texas Tech University School of Veterinary Medicine served as subjects in the study....
Choice for the horse is paramount: Owners’ experiences in facilitating friends, forage, and freedom in their management practices.
Animal welfare (South Mimms, England)    August 10, 2026   Volume 35 e48 doi: 10.1017/awf.2026.10107
Watson WL, Jessiman LJ, Dwyer C.Owned horses rely on people for their behavioural needs, such as access to conspecifics, constant access to low nutrient density, fibrous forage and freedom of movement; otherwise known as friends, forage and freedom (3Fs). Restricting the 3Fs prevents horses from achieving optimal welfare. We carried out 14 semi-structured interviews in the UK and Ireland to examine horse owners' experiences, attitudes and beliefs around the provision of the 3Fs. Most participants were female, aged from 25 to 65 years, resident in the UK and kept their horse at a livery facility. A reflexive thematic analysis...
Four Haylages, One Metabonome: Defining Diet Driven Metabolic Shifts in Ponies.
Journal of animal physiology and animal nutrition    August 6, 2026   doi: 10.1111/jpn.70099
Moore-Colyer M, Harris P, Daniels S.Haylage is widely fed to all categories of stabled horses across Europe and New Zealand. Haylage can vary from high energy nutrient dense forage to low energy highly fibrous fodder, yet little is known about the impact different haylages have on metabolism in horses. This study provides the first direct comparison of feeding four different haylages: early cut (Lolium multiflorum), later cut (Lolium perenne) rye grass (R1 and R2); early and late cut meadow grass (M1 and M2), on urinary and faecal metabonomics, in ponies at maintenance. Using replicated Latin Square design, eight individually ho...
Could snort production reflect comfort in horses kept outdoors? A first study.
Die Naturwissenschaften    June 30, 2026   Volume 113, Issue 4 79 doi: 10.1007/s00114-026-02113-3
Hausberger M, Matthieu L, Delbos M, Briseño-Jaramillo M, Wimel L, Henry S, Lemasson A, Lesimple C.The welfare of free-roaming, captive wild or domestic animals housed outdoors is often questioned. How subjects perceive climatic conditions is not always well detected as it is highly subjective, depending on species, age and experience. Hence, decisions on housing rely mostly on human anthropomorphic perception. In particular, indicators of comfort and positive emotions remain poorly documented. Acoustic signals, however, offer a promising window into animals' internal states. In the present study, we hypothesized that acoustic signals could be used to identify positive or negative perceptio...
Fecal microbiota of weaned equine and mule foals grazing bermudagrass pastures.
Journal of equine veterinary science    June 27, 2026   106070 doi: 10.1016/j.jevs.2026.106070
Araújo LSA, Franzan BC, Almeida MIV, Coelho IS, Almeida FQ.Weaning is a critical period in the lives of foals. After weaning, forage consumption represents a major challenge for both equine or mule foals. Objective: This study evaluated the fecal bacterial concentration, composition, diversity and differential abundance in weaned equine and mule foals under rotational grazing on Bermudagrass (Cynodon spp.) pasture. Methods: A randomized design was used with equine and mule foals grazing two rotations on three Bermudagrass cultivars (Coastcross, Florakirk, and Tifton-85). Pasture rotation occurred every 20 days. The first rotation was evaluated at 20, ...
Forage nutritive analysis in practical equine feeding: challenges and opportunities.
Journal of equine veterinary science    June 18, 2026   106067 doi: 10.1016/j.jevs.2026.106067
Nilsson ST, Müller CE, Lindqvist H.Previous research has shown that forage nutritive analyses are not used to any large extent in equine nutrition. As forages differ widely in nutritional quality, knowledge of the nutritive value is essential to avoid under- or overfeeding of horses. Objective: The aim of this study was to investigate how forage nutritive analyses were used among horse owners in Sweden, and to identify challenges horse owners encounter with such analyses. Methods: Data was collected through an online survey with questions about the respondent, access to forage analytical reports, if and how the respondent used ...
Mastication characterization of horses fed bermudagrass hay in a two-part field study.
Journal of equine veterinary science    June 17, 2026   106068 doi: 10.1016/j.jevs.2026.106068
Long JF, Hutton R, Abrams A, Duberstein JA, Duberstein KJ.Bermudagrass (Cynodon dactylon L.) is a common forage in the southeastern United States but remains controversial in equine diets due to reported associations with impaction colic. Reduced fiber digestibility has been proposed as a contributing factor, however differences in mastication of fine-stemmed bermudagrass hay have not been studied and may play a role in digestive disturbances. Objective: To compare chewing characteristics, including chews per unit weight and chews per minute, in horses consuming bermudagrass hay compared to other common hay types. Methods: Two studies were conducted....
Successful restoration of heathlands and grasslands associated with long-term habitat preferences of cattle and horses: Insights from GPS tracking.
Journal of environmental management    June 9, 2026   Volume 411 130113 doi: 10.1016/j.jenvman.2026.130113
Hendler R, Tischew S, Hensen H, Metze K, Schütz L, Bade K, Fischer C.Long-term, year-round grazing by large herbivores is increasingly used to restore degraded temperate heathlands and grasslands, yet the behavioral mechanisms of grazers underlying restoration remain insufficiently understood. We combined over 13 years of high-resolution GPS-tracking data of free-ranging Heck cattle and Konik horses with repeated vegetation surveys across various habitat types (encroachment-derived Calamagrostis epigejos stands, dry sandy grasslands, dry heaths, and pioneer forests) in Central Germany. We quantified how habitat preference and vegetation structure co-develop und...
Do Polish Konik Horses Find Soaked Hay to Be Palatable?
Animals : an open access journal from MDPI    May 29, 2026   Volume 16, Issue 11 1663 doi: 10.3390/ani16111663
Tkaczyk E, Gazda IE, Humięcka M, Łuszczyński J, Basińska K, Kaczmarek B, Barłowska J, Tkaczyk P, Janczarek I.Frequently, equine diseases necessitate the moistening or soaking of hay for the horses. It was assumed that this treatment would not reduce the willingness of the Polish Konik horses with low nutritional requirements to consume the hay. Furthermore, it was believed that moistening would increase the palatability of this feed. The aim of this study was to determine time and frequency parameters and the amount of unconsumed dry, moist, or soaked hay. The research material comprised 12 Polish Konik horses consuming either 1 kg of dry hay, 2.1 kg of moist hay, or 3.3 kg of soaked hay on three sub...
Open habitats and species differences shape space use in semi-feral cattle and horses across Danish rewilding sites.
Environmental monitoring and assessment    May 23, 2026   Volume 198, Issue 6 639 doi: 10.1007/s10661-026-15487-8
Bergin MD, Börger L, da Rocha ÉG, Havmøller RW, Jensen M, Svenning JC.Large herbivores can strongly influence ecosystem structure and biodiversity, making knowledge of their space use important for trophic rewilding. We used GPS-tracking data collected over two years from 10 semi-feral cattle and 17 horses across ten Danish rewilding sites to analyse habitat use in a use-availability framework using resource selection functions (RSFs), complemented by direct behavioural observations. Both species showed large individual variability but overall used habitats disproportionately to availability, with higher relative use of open habitats; including dry grassland and...
Long-Read Sequencing for Species-Level Resolution of the Equine Gut Microbiota Reveals the Need for Improved Databases.
Animals : an open access journal from MDPI    May 9, 2026   Volume 16, Issue 10 1459 doi: 10.3390/ani16101459
Leduc L, Boucher L, Mach N, Leclère M, Costa M.Differences in gut microbiota composition related to diet have been reported in horses, but characterization of specific microbial taxa remains limited, particularly at the species level. The objective of this study was to use long-read sequencing of the 16S rRNA gene to provide additional taxonomic insight into the intestinal microbiota in horses. Fecal samples were collected from 12 horses on pasture and from 6 of them after switching to a hay diet. Sequencing yielded low read counts per sample, and the analysis failed to detect statistical differences in alpha- and beta-diversity among diet...
Pasture Intake of Whole-Day Stocked Thoroughbred Foals With Growth.
Animal science journal = Nihon chikusan Gakkaiho    April 28, 2026   Volume 97, Issue 1 e70168 doi: 10.1111/asj.70168
Tanabe T, Matsui A, Tominari M, Ueda K, Mitani T, Kawai M.We investigated the changes in the forage and nutrient intake of thoroughbred foals on pastures with whole-day stocking with age. Twelve thoroughbred foals were used and were allowed to graze on a pasture for 21 h/day from June to September. All foals were weaned just before 5 months of age. The pasture intake of the foals was measured using the double-indicator method. During the suckling period, the total voluntary dry matter intake (VDMI) of the pasture for foals was higher at 4 months (2.1 kgDM/day) than at 2 months (1.3 kgDM/day). However, VDMI of pastures per body weight (%BW) for foal...
Survey of equine pasture best management practices and soil fertility in southeast Pennsylvania after the grazing season.
Journal of equine veterinary science    April 11, 2026   105893 doi: 10.1016/j.jevs.2026.105893
Splan RK, Noel FI, Gardner GM, Kenny LB.Proper pasture management improves forage yield, reduces costs, and minimizes environmental impacts, but may be difficult in suburban settings. Objective: This study evaluated pasture condition and management practices on 28 horse farms in counties adjacent to the Philadelphia metropolitan area. Methods: Between November 2019 and January 2020, 72 pastures (2-3/farm) were assessed via soil sampling, USDA-NRCS Pasture Condition Score Sheet (PCSS), Penn State Equine Pasture Evaluation Disc (EPED), and farm manager interviews. Linear models assessed effects of management factors on pasture conditi...
An investigation into the impact of the inclusion of a commercial fiber-based feed, designed for both hydration and nutrition, on the equine hindgut microbiomes.
Journal of equine veterinary science    March 20, 2026   105862 doi: 10.1016/j.jevs.2026.105862
Hart KJ, Hegarty MJ, Hart EH.It is more evident that horse health is linked to the gastrointestinal tract and microbial community structure (MCS) as affected by diet. There are many marketed feeds available to enhance nutrition for horses, however the effects of these feeds on hindgut MCS are largely unknown. Objective: The aim was to test the effect of a complementary forage feed (FF) on the fermentation profile and MCS in contrasting basal diets. Methods: A 2×2 factorial design was used to assess the effect of basal diet, forage only (FOR) or forage/concentrate (MIX), and presence/absence of FF on fermentation paramete...
Clinical nutrition in equine colic: a scoping review from an equine nutritionist’s perspective.
Journal of equine veterinary science    March 14, 2026   105852 doi: 10.1016/j.jevs.2026.105852
Danese T, Asti V, Ablondi M, Raspa F, Cavallini D, Valle E.Colic represents one of the leading causes of morbidity and mortality in equine medicine, with nutritional management recognized as a key modifiable factor influencing postoperative recovery. However, guiding principles for nutritional enteral support in horses after colic remain limited, and current practices are largely empirical. Objective: This scoping review aimed to (1) examine enteral nutritional support protocols reported in literature through a scoping review to map the available evidence on equine clinical nutrition in the context of colic, and (2) propose a physiology-based interpre...
Synergistic effects of Ferula asafoetida extract and condensed tannins from raisin pomace on in vitro cecal fermentation kinetics and nutrient digestibility in horses.
Veterinary world    March 13, 2026   Volume 19, Issue 3 905-919 doi: 10.14202/vetworld.2026.905-919
Dehghan H, Moghaddaszadeh-Ahrabi S, Hashemzadeh-Farhang H, Shahbazi P, Nobari B.The equine hindgut depends on microbial fermentation for efficient nutrient utilization but remains vulnerable to dysbiosis, hindgut acidosis, and suboptimal fiber digestion. Growing restrictions on antibiotic and synthetic feed additives have increased interest in natural phytogenic compounds. Medicinal plant extracts and condensed tannins are promising candidates to modulate microbial activity, improve fermentation efficiency, and enhance nutrient digestibility. This study aimed to investigate the individual and combined effects of hydroalcoholic extract of and condensed tannins extracted f...
Temporal variation in incretin and insulin secretion in ponies in association with dietary macronutrients.
Veterinary journal (London, England : 1997)    March 11, 2026   Volume 317 106644 doi: 10.1016/j.tvjl.2026.106644
Andrews KE, Sibthorpe PEM, Fitzgerald DM, de Laat MA.Equine insulin dysregulation (ID) is a common condition that predisposes to hyperinsulinaemia-associated laminitis. Improved pathophysiological understanding would enable better management of ID and reduce the risk of laminitis. The incretin peptides, glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP), augment the insulin response to dietary glucose and GLP-1 has been implicated in the pathophysiology of ID. However, their temporal variation in response to a low-energy diet has not been reported. This study determined the variation in GLP-1, GIP and insulin ...
Friends, forage, freedom: A cluster analysis investigating horse management styles and welfare in the UK and Ireland.
Animal welfare (South Mimms, England)    February 24, 2026   Volume 35 e15 doi: 10.1017/awf.2026.10073
Watson W, MacKay JRD, Dwyer C.This study aims to improve the welfare and management of recreational horses by identifying how different management styles affect horse health and behaviour. We examined the management styles of recreational horse owners in the UK and Ireland, focusing on social interaction (friends), access to suitable forage (forage), and unrestricted movement (freedom). We collected 1,501 survey responses, distributed via social media, and summarised the characteristics and management choices of the respondents. Using the Divisive ANAlysis cluster package in R, three distinct management styles were identif...
Diet composition influences equine large intestinal motility as measured by transcutaneous ultrasound.
American journal of veterinary research    February 12, 2026   1-6 doi: 10.2460/ajvr.25.11.0400
Starrett A, Arnold CE, Burr A, Sarturi JO, Leatherwood JL, Pilla R, Awosile BB, Chaffin MK.To quantify gastrointestinal motility at 3 anatomic locations of the large colon in healthy horses fed 2 diets. Unassigned: 12 American Quarter Horses were enrolled in a prospective crossover study. Horses received either a complete pelleted diet fed at 1.35% body weight (BW) per day or Coastal Bermudagrass hay fed at 2% BW with 0.5% to 0.7% BW of complete pelleted feed. Each diet was fed for 13 days prior to motility assessment. Two ultrasonographers recorded the frequency of contractions at the sternal flexure of the large colon, left ventral colon, and cecum for 4 consecutive, 1-minute peri...
Blood and fecal metabolic responses of mature horses fed rhizoma peanut hay.
Journal of equine veterinary science    February 11, 2026   Volume 159 105805 doi: 10.1016/j.jevs.2026.105805
Vasco ACCM, Warren LK, Silva GM, Cuervo-Vivas W, Brandi RA, Silva VP, Lance JM, Dubeux JC, Wickens CL.Rhizoma peanut (RP, Arachis glabrata Benth) is an alternative legume forage to alfalfa (ALF, Medicago sativa L.) with less non-structural carbohydrates and crude protein. Yet, metabolic responses to consuming RP hay have never been evaluated in horses. Objective: The objective of this study was to investigate the effect of feeding RP 'Florigraze' hay on fecal and blood metabolic variables compared to alfalfa 'Legendary XHD' and bermudagrass (BMG, Cynodon dactylon L. 'Coastal') hays fed to maintenance horses. Methods: Six mature Quarter Horse geldings (596±40 kg) were randomly assigned to one ...
Feed intake, digestibility and passage kinetics in grazing horses.
Scientific reports    January 22, 2026   Volume 16, Issue 1 3052 doi: 10.1038/s41598-026-35647-7
Bachmann M, Bochnia M, Wensch-Dorendorf M, Glatter M, Schäfer S, Simroth K, Greef JM, Zeyner A.Feed intake and digestibility are important indicators for sufficient nutrient supply, but they can be assessed only to a limited extent in horses on pasture. In horses, feed intake is embedded in a complex behavioural pattern of searching, selecting, chewing and almost constant movement called foraging. The objectives of this study were to estimate organic matter intake (OMI) and organic matter digestibility (OMD) in six horses, 24 h/day on pasture, based on plant alkanes and synthetic n-hexatriacontane (C) excretion. A multi-compartmental model was fitted to the marker excretion and the C m...
Complete Extruded Diet: How Does Equine Fecal Microbiota Change During Intake Adaptation?
Animal science journal = Nihon chikusan Gakkaiho    January 8, 2026   Volume 97, Issue 1 e70147 doi: 10.1111/asj.70147
Franzan BC, da Silva Coelho I, Ramos EM, de Souza ARP, de Almeida FQ, Silva VP.This study aimed to investigate the gradual adaptation of the fecal bacterial community and in vitro fermentative capacity of horses fed a complete extruded diet (CED). Twelve geldings weighing 370 kg were removed from a native pasture and fed coastcross hay (Cynodon spp) for 7 days. In the second week, horses were assigned to two groups: one group was fed exclusively with coastcross hay (HAY) for 28 days and the other group fed with weekly increases of CED (30%, 60%, and 100%) to replace HAY. Fecal samples were collected on Days 7, 14, 21, and 28 for microbiota and in vitro fermentati...
The use of hay nets and slow feeders as feeding methods in horse management: A semi-systematic review.
Journal of equine veterinary science    December 30, 2025   Volume 157 105762 doi: 10.1016/j.jevs.2025.105762
Amaje J, Upton S, Garba UM, Jolayemi KO.Hay nets and slow feeders are designed to mimic natural grazing by prolonging forage intake, thereby supporting digestive health and reducing stress-related behaviours. Their design and use, however, can influence effectiveness and may introduce risks. Objective: This semi-systematic review evaluated the use of hay nets and slow feeders in feeding horses, focusing on their impact on feeding time, behaviour, forage management and health management. Methods: A semi-systematic search across PubMed, ScienceDirect, Google Scholar, and Scopus identified 26 full-text studies published between 2009 an...
Effects of Feeding Strategies on Gut Microbial Communities in Donkeys: A Comprehensive Narrative Review.
Veterinary sciences    December 20, 2025   Volume 13, Issue 1 7 doi: 10.3390/vetsci13010007
Wei L, Wei J, Liu X, Chen W, Wang C, Khan MZ, Zhang Z.Donkeys () remain nutritionally understudied despite their critical roles in agriculture across developing regions, with current feeding practices inappropriately extrapolating horse standards without accounting for species-specific digestive physiology. No comprehensive synthesis has integrated how dietary modifications systematically alter gut microbial communities to drive measurable health outcomes in donkeys, preventing development of evidence-based feeding strategies. This review critically synthesizes current evidence on donkey nutritional requirements and gut microbiota dynamics to est...
Using Satellite Remote Sensing to Estimate Rangeland Carrying Capacity for Sustainable Management of the Marismeño Horse in Doñana National Park, Spain.
Animals : an open access journal from MDPI    December 5, 2025   Volume 15, Issue 24 3507 doi: 10.3390/ani15243507
Ramírez-Juidias E, Díaz de la Serna-Moreno Á, Delgado-Pertíñez M.Rangeland degradation poses a serious challenge for the sustainable management of free-ranging livestock in Mediterranean wetlands. In National Park, Spain, the endangered horse depends exclusively on natural forage, making it essential to monitor vegetation productivity and grazing suitability under increasing climate variability. This study presents a satellite-based assessment of rangeland carrying capacity to support the adaptive management of this iconic breed. A six-year time series (2015-2020) of 1242 images from Landsat 8 OLI/TIRS and Sentinel-2 (L1C/L2A) was processed using ILWIS an...
The Role of Short-Chain Fatty Acids (SCFAs) in Colic and Anti-Inflammatory Pathways in Horses.
Animals : an open access journal from MDPI    December 3, 2025   Volume 15, Issue 23 3482 doi: 10.3390/ani15233482
Schank N, Cottone A, Wulf M, Seiter K, Thomas B, Miller LMJ, Anderson SL, Sahyoun A, Abidi AH, Kassan M, Verma A.Equine colic remains a prevalent and potentially life-threatening condition with multifactorial origins, including dietary imbalances, stress, and microbial dysbiosis. Central to equine gut health is the production of short-chain fatty acids (SCFAs), primarily acetate, propionate, and butyrate, generated through microbial fermentation of dietary fibers in the hindgut. These metabolites not only serve as vital energy sources but also play crucial roles in maintaining intestinal barrier integrity, modulating motility, and suppressing inflammation. This review explores the role of SCFAs in equine...
Forage Carbohydrate Profiles and Endocrine Morphometric Interactions in Traditionally Managed Horses from Romania.
Life (Basel, Switzerland)    November 6, 2025   Volume 15, Issue 11 1721 doi: 10.3390/life15111721
Horses maintained under traditional management systems and dependent on natural forages are often exposed to seasonal and compositional variations that can affect metabolic homeostasis. This study examined associations between forage nutrient composition and metabolic-morphometric indicators in horses from four agroecologically distinct regions of northwestern Romania. Eighty-eight horses managed under semi-extensive rural conditions underwent clinical examination, body condition scoring (BCS), cresty neck scoring (CNS), and fasting blood sampling. Forage samples ( = 34) from daily rations wer...
Time Budgets in Domesticated Male Icelandic Horses on Pasture Turnout in Winter and Spring.
Animals : an open access journal from MDPI    November 4, 2025   Volume 15, Issue 21 3206 doi: 10.3390/ani15213206
Taylor DEF, Lancaster BE, Ellis AD.There are few 24 h time budgets for horses, especially for domesticated horses kept at pasture. Most time budgets utilise short-term scan sampling, which can miss behaviours. This study aimed to assess the seasonal variation in continuous behaviour of domesticated Icelandic horses at pasture during winter and spring in fair weather. Eight Icelandic horses (11.25 ± 9.19 years; 7 geldings, 1 stallion) were observed in a 26 acre field. Herd location and individual behaviour were continuously observed during 3 h periods amounting to 3 × 24 h in winter and late spring, compiled over 43 days (~21 ...
A comparison of fecal output determined by total fecal collection or titanium dioxide in horses.
Journal of animal science    October 28, 2025   Volume 103 skaf375 doi: 10.1093/jas/skaf375
Fowler AL, Pyles MB, Hayes SH, Crum AD, Lawrence LM.Total fecal collection studies to determine digestibility of nutrients are costly and laborious. The use of externally dosed indigestible markers, such as titanium dioxide (TiO2), to estimate digestibility using spot samples could be advantageous, but studies validating their use in horses are inadequate. Two experiments were conducted to determine if TiO2 in fecal spot samples effectively estimated fecal output in horses. In Exp. 1, four mature horses were fed a forage-based diet (85:15 forage: concentrate) split into two equal meals with 1.75 ± 0.03 g TiO2/kg DM (10 g TiO2) per day ...
The metabolomic profile of a high starch versus no starch diet in athletic horses.
Scientific reports    October 13, 2025   Volume 15, Issue 1 35576 doi: 10.1038/s41598-025-23422-z
Nilsson E, Moazzami AA, Lindberg JE, Jansson A.Feeding a high amount of starch-rich grains is common practice for performance horses even though the horse has evolved to eat a grass based, i.e. low starch diet. To our knowledge, there are no studies using metabolomics to investigate the effects of a high-starch diet in horses. In this study we investigated differences in the plasma metabolic profile of 6 Standardbred horses fed a no-starch, forage-only (F) diet or a high-starch forage-concentrate (FC) diet for 29 days, respectively in a cross-over design. Postprandial plasma samples were collected on the morning of day 25 of each dietary ...
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