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Topic:Observational Study

Observational studies in horses involve systematically assessing and recording behaviors, conditions, or other characteristics without manipulating the study environment. These studies provide insights into natural equine behaviors, health conditions, and interactions with their environment. Researchers often focus on aspects such as herd dynamics, feeding patterns, or responses to environmental changes. The data collected can help identify trends, correlations, and potential risk factors for certain conditions. This page compiles peer-reviewed research studies and scholarly articles that explore various observational methodologies and their applications in understanding equine behavior and health.
Intra-rater and inter-rater reliability of ultrasonographic intestinal wall thickness measurements in healthy horses.
Equine veterinary journal    January 4, 2026   doi: 10.1002/evj.70147
Hansen T, Kendall A, Finne R, Law E, Ringdahl A, Nostell K.Abdominal ultrasound is widely used to evaluate the intestinal tract of horses. Despite being a routine examination, there is limited data on the reliability of this diagnostic procedure. Objective: To investigate intra- and inter-rater reliability of ultrasonographic intestinal wall thickness measurements in healthy horses. A second aim was to assess variance within repeated measurements to determine threshold values that distinguish whether differences between repeated examinations are true findings versus solely due to measurement variation. Methods: In vivo reliability study. Methods: Eigh...
Natural progression of tarsal osteochondrosis in Standardbred pacers and trotters.
Veterinary surgery : VS    January 3, 2026   doi: 10.1111/vsu.70073
McCoy AM, Lopp-Schurter CT, Bishop RC, Narotsky A, Grogger K, Kemper AM.To determine the natural progression of tarsal osteochondrosis (OC) in a cohort of Standardbred foals and assess the impact of gait preference (trotting vs. pacing). Methods: Longitudinal observational cohort study. Methods: Client-owned Standardbred foals (n = 148). Methods: Tarsal radiographs were taken every 2 months from 2 to 12 months of age and foals were video monitored to document time spent pacing or trotting. Differences between groups were assessed using χ analysis. Survival analysis was used to determine if lesion healing differed between groups over time. Results: Of 148 ...
Weekly riding frequency has a greater impact than rider skill level on western riders’ identification of stirrup asymmetry.
Journal of equine veterinary science    January 2, 2026   Volume 157 105758 doi: 10.1016/j.jevs.2025.105758
Jolley EN, Higgins AH, Logan AA.Asymmetry of stirrups and rider experience level has shown to impact rider symmetry and position. Objective: This study aims to determine if novice and advanced riders can detect stirrup asymmetry in the same frequency, hypothesizing that advanced riders would detect asymmetry in a greater frequency. Methods: Ten stock-type horses and 10 (5 advanced, 5 novice) riders, paired together to test random stirrup treatments. Riders were assigned to a horse for 2 rides, one with asymmetric stirrups, the other with symmetric stirrups, but blinded to their treatments. At the end of both rides, they took...
Longitudinal welfare assessment in French jump racehorses during season preparation.
Equine veterinary journal    December 21, 2025   doi: 10.1002/evj.70142
Bonhomme MM, Boisdenghien L, Couroucé A, Votion DM.Public scrutiny of racehorse welfare is increasing. The preparatory training phase preceding the racing season is potentially a critical period for physical and mental development. Structured welfare assessment protocols have recently been developed, but their use in field conditions remains limited. Objective: (1) To evaluate the field applicability of a racehorse-specific welfare assessment protocol in a professional French jump racing yard; and (2) to explore whether it can detect relevant physical and behavioural changes in young horses during season preparation. Methods: Longitudinal obse...
Development of the Human-Equine Attachment Scale.
Equine veterinary journal    December 16, 2025   doi: 10.1002/evj.70141
Corrigan RH, Pierard M, Davies E, Marlin D, Evans S, Williams JM.Human-horse relationships encompass diverse roles, from companion to competition partner. The impact of such bonds informs owner decision-making regarding horse management and veterinary care, yet standardised instruments to measure these unique bonds are limited. Objective: To develop the Human-Equine Attachment Scale (HEAS), a novel instrument to measure the multi-faceted dimensions of human-horse attachment. Methods: Cross-sectional design using a self-administered psychometric instrument. Methods: Initial items were developed through a systematic review of human and animal attachment resea...
Horses show limited owner bias in reunion and odor tests: a pilot study.
Journal of equine veterinary science    December 16, 2025   Volume 156 105755 doi: 10.1016/j.jevs.2025.105755
Rönnow E, Roth LSV.Compared to other companion animals, our understanding of the human-horse relationship is limited, particularly from the horse's perspective. This pilot study examined whether horses (N = 30) show a bias towards their owner in a reunion in the presence of both the owner and a stranger. Afterwards, the horses were also presented with the odor of the owner and a stranger. Both tests were video recorded for behavioral analyzation. No significant owner-directed bias was observed at the group level in either the reunion test or the odor test. Nonetheless, in the reunion test, owner bias in physical...
Measuring emotional contagion in Horse-Human interactions: A systematic scoping review of methods and outcomes.
Journal of equine veterinary science    December 16, 2025   Volume 156 105754 doi: 10.1016/j.jevs.2025.105754
Tripon MA, Manolăchescu D, Papuc I, Daradics Z, Crecan CM.Emotional contagion between horses and humans is a key aspect of their interaction, influencing welfare, training, and therapy, yet current methods for measuring this phenomenon lack standardization and consistency. Objective: The aim of this study was to verify what methods are used to assess emotional contagion in horse-human interactions, and what outcomes have been reported. Methods: A systematic search of Google Scholar, Web of Science, Scopus, PubMed, and Science Direct was conducted up to March 2024. Peer-reviewed studies assessing emotional transfer through behavioral and/or physiologi...
A Systematic Narrative Review of Stable Staff and Trainers’ Occupational Health and Well-Being in Thoroughbred Horse Racing: An International Perspective.
Workplace health & safety    December 16, 2025   Volume 74, Issue 4 164-174 doi: 10.1177/21650799251388471
Tobin G, Cullen S, Dunne A, Warrington G, Pugh J, McGoldrick A, Nolan C, Losty C.Stable staff and trainers are essential to racing yards and horse welfare, yet limited research focuses on this vital workforce that the thoroughbred industry heavily relies on. This systematic narrative review synthesizes existing literature on the occupational health and well-being of stable staff and trainers in the thoroughbred horse racing industry. Methods: A systematic narrative review was conducted across PubMed, Google Scholar, and Scopus using relevant keywords. Manuscripts reporting occupational or mental health data on stable staff and/or trainers and had the full-text available we...
Preliminary assessment of the leukocyte coping capacity as a point of care marker in horses with stress associated diseases.
BMC veterinary research    December 7, 2025   doi: 10.1186/s12917-025-05179-9
Jandová V, Huber N, AlMheiri FG, Bábor K, Trachsel DS.Stress represents a serious health and welfare concern; however, its objective assessment remains difficult. The equine gastric ulcer syndrome (EGUS) and orthopedic diseases that cause pain are among stress associated diseases in equine medicine. The leukocyte coping capacity (LCC) quantifies oxygen radical generation of neutrophil granulocytes which is altered under stress. Therefore, LCC could be a novel biomarker for stress in horses and we hypothesized that horses with stress associated diseases would have lower LCC values in comparison to horses without these diseases. Methods: In this ob...
Preliminary investigation of equine veterinary hospital staff attitudes towards pain assessment in a single centre.
The Veterinary record    December 4, 2025   doi: 10.1002/vetr.6122
Curry O, Everett A, Pearson G, Dwyer C, Duncan J.Despite the availability of several equine pain assessment tools, their use in equine veterinary practice appears limited compared to small animal practice. This study explores potential barriers to equine pain assessment, as reported by staff at a single UK equine teaching hospital. Methods: Nine hospital staff members were interviewed using semi-structured interviews. Key themes were identified through reflexive thematic analysis. Results: Participants acknowledged the importance of pain assessment, yet highlighted limitations in current methods and their inconsistent use. Key challenges inc...
The effect of full-limb flexion tests on static and dynamic muscle activity and locomotion asymmetry in owner-sound horses.
Equine veterinary journal    December 2, 2025   doi: 10.1111/evj.70130
Jonkhart M, Serra Bragança FM, Smit IH, Brommer H, Suskens JJM.Flexion tests are commonly used in equine locomotion examinations to identify underlying locomotor issues, yet their neuromuscular effects remain poorly understood. Response variability raises concerns about their clinical value in lameness assessments and pre-purchase evaluations. Objective: Primarily, to investigate the effect of full-limb flexion tests on static (flexed position) and dynamic (subsequent trot-up) muscle activity. Secondarily, to assess their effect on locomotion asymmetry during trotting. Methods: In vivo experiments. Methods: Sixteen warmblood horses were randomly assigned ...
Localisation and outcome of presumed thoracolumbar vertebral stress fracture in 35 UK Thoroughbred racehorses using nuclear scintigraphy.
Equine veterinary journal    December 1, 2025   doi: 10.1111/evj.70113
Chapple AR, Daglish J, Stover SM, Slipchenko N, Phillips KL.Stress fracture is an important cause of mortality and morbidity in racing Thoroughbreds. Nuclear scintigraphy is the imaging modality of choice for investigating unlocalised stress-related bone remodelling in horses. Stress fractures of the caudal lumbar vertebral column have been previously described in both racing Quarter horses and racing Thoroughbreds post-mortem. There is a lack of literature describing the imaging appearance of vertebral stress fractures in racehorses ante-mortem. Objective: To describe: (1) ante-mortem scintigraphic appearance of presumed thoracolumbar vertebral stress...
A Pilot Observational Study in Ohio, USA of the Healing of Our Veterans Equine Services Intensive Intervention for Veterans with Trauma Histories.
Healthcare (Basel, Switzerland)    November 28, 2025   Volume 13, Issue 23 3111 doi: 10.3390/healthcare13233111
Held A, Hubbard K, Nazarenko E, Marchand W.: Equine-assisted services are being increasingly utilized as complementary interventions for military veterans who have experienced trauma. However, rigorous research is lacking, and randomized controlled trials are needed. The H.O.O.V.E.S. Intensive intervention was developed for this population. This intensive program is an equine-assisted learning approach developed for veteran and active-duty military trauma survivors. The program integrates equine-assisted learning, peer mentorship and experiential learning in a residential retreat format. The primary aim of this pilot study was to deter...
A systematic review on the effect of individual characteristics and management practices on equine cognition.
Animal cognition    November 26, 2025   Volume 28, Issue 1 96 doi: 10.1007/s10071-025-02016-2
Equine cognition is relevant to the many roles that horses serve in society, such as leisure riding, competitions, or even animal-assisted therapy. Equine cognitive abilities have been explored in recent years. However, gaining an overview of horse cognition is challenging due to the broad range of abilities studied and the diverse methodologies employed. In addition, the subjects of existing equine cognition studies vary greatly in contextual factors such as their breed, age, sex, and management conditions - each of which may influence test performance in the following cognitive categories: D...
Equine-assisted learning and leadership transformation: an exploratory qualitative study of workplace behavior.
Frontiers in veterinary science    November 25, 2025   Volume 12 1700029 doi: 10.3389/fvets.2025.1700029
Sivagurunathan R, Senathirajah ARBS, Sivagurunathan L, Arokiasamy L, Qazi S, Haque R, Su Y.This study explores how equine-assisted leadership development (EALD) interventions activate experiential processes that reshape leaders' self-concept, relational schemas, and behaviors. A conceptual model is proposed to explain how non-verbal interaction with horses catalyzes transformational learning. Unassigned: A qualitative exploratory design was employed to examine leaders' experiences over 12 months following reintegration into their workplaces. Eight leaders ( = 8) attended a 5-day EALD program, engaging in "join-up" exercises with horses. Data were analyzed through reflexive the...
Child horse harmony in motion: a preliminary study to explore heart rate synchronization in equine assisted therapy for neurotypical and ADHD children.
Scientific reports    November 25, 2025   Volume 15, Issue 1 45312 doi: 10.1038/s41598-025-29330-6
Helmer A, Hacohen A, Bart O.Equine-Assisted Services (EAS) encompass a range of therapeutic interventions utilizing equine interactions to achieve therapeutic goals. This study explores heart rate synchronization between horses and riders during mounted and unmounted interactions, focusing on its potential implications for emotional regulation. A total of 25 participants aged 6-12 took part in the study, which included two groups: novice riders diagnosed with Attention Deficit Hyperactivity Disorder (ADHD) (n = 15) and experienced neurotypical riders (n = 10). Heart rate measurements were obtained using Polar Equ...
Preliminary Assessment of Leisure Horses’ Preferences for Different Forms of Carrot (Daucus carota subsp. sativus).
Animals : an open access journal from MDPI    November 24, 2025   Volume 15, Issue 23 3385 doi: 10.3390/ani15233385
Mańkowska A, Dobraczyńska BM, Szewczak J, Chodup Z, Radzanowski B, Matychyn I, Witkowska D.Cultivated carrot ( subsp. ) is a valuable component of equine diets due to its high β-carotene content (a precursor of vitamin A) and desirable sensory properties. However, its use may be limited by short shelf life, susceptibility to spoilage, and firm texture, which can be challenging for horses with dental problems. This study aimed to evaluate horses' preferences for different physical forms of carrots. Twenty-one leisure horses aged 3-22 years were individually evaluated in a free-choice test. Each horse was simultaneously offered equal portions (150 g) of five carrot forms: raw, grated...
Preliminary Biometric Study on Symmetry of Hoof Solear Aspect in Forelimbs in Four Horse Breeds.
Animals : an open access journal from MDPI    November 21, 2025   Volume 15, Issue 23 3369 doi: 10.3390/ani15233369
Stachurska A, Wnuk E, Łuszczyński J, Donderowicz W.Correct conformation and symmetry of bilateral hooves are associated with a properly balanced hoof, which has a significant impact on the biomechanics of movement and reduces the risk of injury. The aim of the study was to compare solear aspect dimensions of bilateral hooves in forelimbs (hoof width, length, frog width, length, medial and lateral diagonals) with regard to sex, breed and age of horses. The dimensions were measured with a calliper in 100 horses of four breeds. The results showed that the breed factor significantly affected all dimensions. Age was significant for some variables, ...
Opinions of trainers of Warmblood, Thoroughbred and Trotter horses in Germany on the pretraining management of yearlings and two-year-old horses – a qualitative analysis.
Journal of equine veterinary science    November 20, 2025   Volume 156 105736 doi: 10.1016/j.jevs.2025.105736
Pilger F, Aurich C, Aurich JE.The early career start of racehorses, the pretraining of two-year-old Warmblood stallions for licensing, and individual housing of young horses increasingly raise animal welfare concerns. Objective: Assess pretraining practices and the opinion of trainers on housing of young horses, minimum age to commence training and pretraining injuries. Methods: Qualitative interviews with trainers of Warmblood stallions and Thoroughbred and Trotter racehorses (n = 10 each). Results: Pretraining of Thoroughbreds and Trotters began at 18 months, whereas licensing preparation of Warmblood stallions began at ...
“If you trust a vet, it’s easier to reach out to them with questions”: Flemish horse owner perspectives on communication about complementary and alternative veterinary medicine.
American journal of veterinary research    November 19, 2025   Volume 87, Issue 2 ajvr.25.03.0091 doi: 10.2460/ajvr.25.03.0091
Keller P, Vanwesenbeeck I, Hudders L, Decloedt A.To assess horse owners' behaviors and perceptions regarding communication about complementary and alternative veterinary medicine (CAVM) with their veterinarian. Unassigned: A cross-sectional online survey was combined with a qualitative study in the form of in-depth interviews with Flemish horse owners. Unassigned: The study included 1,532 fully completed survey responses and 26 interviews. Overall, horse owners showed high trust in the veterinarian and were satisfied with veterinarian-client communication. While 61.7% of participants indicated that they had already talked about CAVM with the...
Agreement between veterinarians and three objective evaluation systems in naturally occurring equine lameness.
Equine veterinary journal    November 16, 2025   doi: 10.1111/evj.70116
McPeek JL, Menarim B, Sponseller B, McClendon M, Adam EN, Adams AA, Slone S, Page AE.Subjective lameness evaluations are critical components of equine musculoskeletal health assessments. Objective approaches can supplement diagnosis and may be preferred for specific cases and scientific purposes. Objective: Evaluate agreements between subjective evaluation of two veterinarians and standard clinical interpretations from outputs of an AI-based smartphone application (Sleip; AI-SPA), the inertial measurement unit (IMU) system Equinosis Q Lameness Locator (LL), and the IMU system Equisym (ES). Methods: In vivo experiment. Methods: Twenty-five research horses (10-30 years) were e...
Chronic State and Relationship to Humans Influence How Horses Decode Emotions in Human Voices: A Brain and Behavior Study.
Animals : an open access journal from MDPI    November 5, 2025   Volume 15, Issue 21 3217 doi: 10.3390/ani15213217
d'Ingeo S, Siniscalchi M, Quaranta A, Cousillas H, Hausberger M.Current research on acoustic encoding of emotional content suggests that there are universal cues, allowing for decoding within and across taxa. This is particularly important for human-animal relationships, wherein domestic animals are supposed to be particularly efficient in decoding human emotions. Here we investigated whether the decoding of the emotional content in human voices shared universal acoustic properties, or whether it could be influenced by experience. Emotional human voices were presented to two populations of horses, in which behavioral, cardiac, and brain responses were meas...
Reliability, agreement and variability of a markerless computer vision algorithm for equine gait analysis under field conditions.
Equine veterinary journal    November 4, 2025   doi: 10.1111/evj.70109
Key K, Berg K, Kirkegaard J, Andresen KR, Skov Hansen S.Computer vision-based algorithms offer accessible alternatives for equine gait analysis but require thorough assessment under diverse conditions. Objective: To evaluate a proprietary vision-based algorithm's reliability in measuring vertical displacement signals (VDS) at the eye, withers and croup, alongside groundline estimation, for horses trotting on straight lines and circles under field conditions. Methods: Cross-sectional comparative study evaluating agreement, variability and reliability of a markerless computer vision algorithm. Methods: We obtained 67 handheld iPhone recordings from 3...
Serum proteomic analysis in equine obstructive colic: A prospective observational study.
Veterinary journal (London, England : 1997)    November 4, 2025   Volume 314 106477 doi: 10.1016/j.tvjl.2025.106477
The aim of the study was to identify proteomic signatures from the serum of horses affected by simple obstructive intestinal colic to characterize the pathological process and to assess potential biomarkers for early diagnosis. Seven horses with obstructive colic received venous blood samples for determination of standard hematobiochemical, inflammatory, and lipid profiles at the time of initial clinical examination and after conservative therapy upon recovery. Proteomic profiling was also performed on all samples by means of a within-group analysis (sick horses at discharge vs. sick horses at...
Time Budgets in Domesticated Male Icelandic Horses on Pasture Turnout in Winter and Spring.
Animals : an open access journal from MDPI    November 4, 2025   Volume 15, Issue 21 3206 doi: 10.3390/ani15213206
Taylor DEF, Lancaster BE, Ellis AD.There are few 24 h time budgets for horses, especially for domesticated horses kept at pasture. Most time budgets utilise short-term scan sampling, which can miss behaviours. This study aimed to assess the seasonal variation in continuous behaviour of domesticated Icelandic horses at pasture during winter and spring in fair weather. Eight Icelandic horses (11.25 ± 9.19 years; 7 geldings, 1 stallion) were observed in a 26 acre field. Herd location and individual behaviour were continuously observed during 3 h periods amounting to 3 × 24 h in winter and late spring, compiled over 43 days (~21 ...
Radiographic measurements of Miniature Horse and Miniature Pony Feet.
Journal of equine veterinary science    October 31, 2025   Volume 155 105710 doi: 10.1016/j.jevs.2025.105710
Olijnyk JM, Hosgood G, Richardson J, Beggs C, McConnell E.Reliable assessment of foot conformation is essential for podiatric management in Miniature Horses and Ponies, yet reference ranges and the reliability of radiographic measurements remain poorly defined. Objective: This study quantified radiographic measurements of foot conformation in sound, skeletally mature Miniature Horses and Ponies and evaluated their agreement, reliability, and precision. Methods: Standardised lateromedial and dorsopalmar/plantarodorsal radiographs were obtained from 30 Miniature Horses and Ponies. Two observers took 60 measurements from 10 sets of projections on three ...
Player-Reported Perceptions of Lameness Risks and Contributing Factors for Polo Horses: Results from a Survey.
Animals : an open access journal from MDPI    October 29, 2025   Volume 15, Issue 21 3136 doi: 10.3390/ani15213136
Schumacher A, Merle R, Stöckle S, Gehlen H.Polo is an equestrian sport with a long tradition, but to date there is little scientific data on health risks, particularly lameness. In an international online survey, 145 polo players (47.6% female, 52.4% male) with an average of 13.4 years of playing experience were questioned about lameness risks and influencing factors. The respondents reported a median of six horses, most frequently mares as their best-performing and healthiest animals. Horse age was significantly associated with the risk of lameness, increasing by approximately 19.5% per year (OR = 1.195; = 0.030) and differed dependi...
A comparison of fecal output determined by total fecal collection or titanium dioxide in horses.
Journal of animal science    October 28, 2025   Volume 103 skaf375 doi: 10.1093/jas/skaf375
Fowler AL, Pyles MB, Hayes SH, Crum AD, Lawrence LM.Total fecal collection studies to determine digestibility of nutrients are costly and laborious. The use of externally dosed indigestible markers, such as titanium dioxide (TiO2), to estimate digestibility using spot samples could be advantageous, but studies validating their use in horses are inadequate. Two experiments were conducted to determine if TiO2 in fecal spot samples effectively estimated fecal output in horses. In Exp. 1, four mature horses were fed a forage-based diet (85:15 forage: concentrate) split into two equal meals with 1.75 ± 0.03 g TiO2/kg DM (10 g TiO2) per day ...
Determinants of stride parameters in Thoroughbreds racing in Japan.
American journal of veterinary research    October 28, 2025   Volume 87, Issue 2 ajvr.25.07.0273 doi: 10.2460/ajvr.25.07.0273
Takahashi Y, Pfau T, Tsuruoka F, Yoshida T, Edwards WB, Mukai K.To identify the race- and horse-level factors affecting stride parameters during Thoroughbred races in Japan. Unassigned: Global Navigation Satellite System sensors were attached to 921 horses (1,189 starts) participating in 83 races, with distances ranging from 1,000 to 1,800 m, held from April through July 2024. Stride frequency and stride length were calculated from speed spectrograms at 3 racing phases (phase 1, 200 m after gate open; phase 2, 10 m after reaching the final straight stretch; and phase 3, 130 m before the finishing line). Additionally, 10 variables (race distance, surface ty...
Impact of sequential administration of detomidine, butorphanol, and midazolam on sedation, ataxia, stimulus response, and bispectral index in horses.
Frontiers in veterinary science    October 23, 2025   Volume 12 1691137 doi: 10.3389/fvets.2025.1691137
Thorn CA, Wilson DV, Wang S, Horne WA.Standing sedation is a safe and cost-effective alternative to general anesthesia in horses, but challenges include achieving adequate drug effect to block the stimulus response without inducing ataxia or recumbency. A benefit of midazolam has been reported in equine dental procedures. Seeking synergy, a combination of lower doses of several pharmacologic agents, including midazolam, may improve the quality of sedation while minimizing adverse effects. Bispectral index (BIS) correlates with sedation scores in human ICU patients, but the correlation between sedation scores and BIS has not been e...