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Topic:Rehabilitation

Rehabilitation for horses refers to the process of restoring physical function and performance following injury, illness, or surgery. This process often involves a combination of veterinary care, physical therapy, and specialized training techniques. Rehabilitation aims to promote healing, improve mobility, and prevent further injury. Common methods used in equine rehabilitation include controlled exercise programs, hydrotherapy, and the use of therapeutic modalities such as laser therapy and ultrasound. The effectiveness of these methods is evaluated through clinical assessments and performance metrics. This page compiles peer-reviewed research studies and scholarly articles that explore the methodologies, outcomes, and advancements in the field of equine rehabilitation.
The impact on physical performance, pain and psychological wellbeing of chronic low back pain patients during 12-weeks of equine- facilitated therapy intervention.
Frontiers in veterinary science    March 14, 2023   Volume 10 1085768 doi: 10.3389/fvets.2023.1085768
Mattila-Rautiainen S, Venojärvi M, Rautiainen H, Keski-Valkama A.Equine - Facilitated Therapy (EFT), an equine environment, and horses themselves can meet many physical and mental health needs beyond diagnostic categories. The horse's ability to produce a walk-like movement and the participant's ability to connect to non-judgemental living creatures, both of which can benefit participation and construct a positive self-image for chronic pain patients. The objective of this study is to evaluate the impact of EFT on perceived physical performance, level of pain, pain acceptance, depression and anxiety, and quality of life within a 12-week intervention for chr...
Correlation of Arthroscopic Grading and Optical Coherence Tomography as Markers of Early Repair and Predictors of Later Healing Evident on MRI and Histomorphometric Assessment of Cartilage Defects Implanted with Chondrocytes Overexpressing IGF-I.
Cartilage    March 2, 2023   Volume 14, Issue 2 210-219 doi: 10.1177/19476035231154508
Ciamillo SA, Pownder SL, Potter HG, Stefanovski D, Nixon AJ, Ortved KF.Injury of articular cartilage is common, and due to the poor intrinsic capabilities of chondrocytes, it can precipitate joint degradation and osteoarthritis (OA). Implantation of autologous chondrocytes into cartilaginous defects has been used to bolster repair. Accurate assessment of the quality of repair tissue remains challenging. This study aimed to investigate the utility of noninvasive imaging modalities, including arthroscopic grading and optical coherence tomography (OCT) for assessment of early cartilage repair (8 weeks), and MRI to determine long-term healing (8 months). Large (15 mm...
Approaches to standardising the magnetic resonance image analysis of equine tendon lesions.
Veterinary record open    February 23, 2023   Volume 10, Issue 1 e257 doi: 10.1002/vro2.57
Doll CU, Bohner M, Berner D, Buettner K, Horstmeier C, Winter K, Burk J.Low-field magnetic resonance imaging (MRI) has gained increasing importance to monitor equine tendon lesions. Comparing results between studies and cases is hampered, because image analysis approaches vary strongly. This study aimed to improve reliability, comparability and time efficiency of quantitative MRI image analysis. Unassigned: Induced tendon lesions were studied over a 24-week period with 10 follow-up MRI examinations. Signal intensities (SIs) of tendons, tendon lesions, cortical bone and background, as well as lesion cross-sectional areas (CSAs) were measured. Lesion SI standardisat...
A Preliminary Study of the Influence of High Intensity Laser Therapy (HILT) on Skin Surface Temperature and Longissimus Dorsi Muscle Tone Changes in Thoroughbred Racehorses with Back Pain.
Animals : an open access journal from MDPI    February 22, 2023   Volume 13, Issue 5 doi: 10.3390/ani13050794
Zielińska P, Soroko-Dubrovina M, Dudek K, Ruzhanova-Gospodinova IS.The reason for undertaking this study was to investigate soft tissue response to high-intensity laser therapy (HILT) by measuring changes in skin surface temperature and longissimus dorsi muscle tone in the thoracolumbar back area in Thoroughbreds with back pain and diagnosed with and without Kissing Spines Syndrome (KSS). Thoroughbreds aged 3-4 years with clinically presented back pain underwent a radiological examination (to assess a lack or presence of KSS) and longissimus dorsi muscle palpation (to assess muscle tone and pain degree). The subjects were divided into two groups, those with K...
Breast Cancer: How Hippotherapy Bridges the Gap between Healing and Recovery-A Randomized Controlled Clinical Trial.
Cancers    February 19, 2023   Volume 15, Issue 4 1317 doi: 10.3390/cancers15041317
Viruega H, Galy C, Loriette C, Jacquot S, Houpeau JL, Gaviria M.Breast cancer is the most diagnosed women's cancer, and has a high survival rate. Despite great progress in detection and treatment, life reconstruction requires comprehensive cross-sectoral approaches between different disciplines and deeper consideration of the patient's challenges. Hippotherapy is an emerging specialized rehabilitation approach, performed by accredited health professionals and equine specialists, on specially trained horses via their movement, activating major paths for physical, mental, psychic and social reinforcement, and is synergistic to rehabilitative and supportive c...
Severe equinus cavo-varus correction through triplanar tarsectomy and posterior tibial tendon transfer based on 3D-printed biomodel.
Foot (Edinburgh, Scotland)    February 11, 2023   Volume 54 101976 doi: 10.1016/j.foot.2023.101976
Chans-Veres J, Albiñana-Cunningham J, Quintela JM, Pereira E, Tejero S.Surgery for severe equine cavo-varus is complex and must be individualized. The interindividual phenotypic variability demands a personalized planning of each foot to be operated. The study's primary goal was to evaluate the function and satisfaction of a series of patients with severe equinus cavo-varus deformity who underwent a triplanar tarsectomy and transposition of the posterior tibial tendon in a single stage surgery after a patient specific 3D biomodel planning. A series of 12 feet (5 patients bilaterally) operated with this technique was analyzed. The cohort comprised 2 females (28.5 ...
Reattachment of Osteochondritis Dissecans Lesions in the Lateral Femoral Trochlear Ridge With Bioabsorbable Screws in 4 Yearling Standardbreds.
Journal of equine veterinary science    February 10, 2023   Volume 123 104242 doi: 10.1016/j.jevs.2023.104242
Bertuglia A, Pallante M, Pillon G, Valle D, Pagliara E, Riccio B.This case series describes an alternative surgical technique to obtain reattachment of osteochondritis dissecans (OCD) lesions in the lateral trochlear ridge of the femur (LTRF) as well as the clinical and radiological outcome of treated cases. Four Standardbred yearlings (6 lesions in total) underwent surgical fixation of large OCD defects in the LTRF under arthroscopic guidance. Reattachment of the OCD lesions was obtained using 3.0/3.7 mm headless bio-compression and absorbable poly-l-lactic acid (PLLA) screws, inserted perpendicularly to the cartilage surface through the lesion. All horse...
Horseback Riding-Related Injuries in Portugal and Prevention Strategies.
Journal of sport rehabilitation    January 23, 2023   Volume 32, Issue 4 409-414 doi: 10.1123/jsr.2022-0101
Pinto LV, Gouveia FC, Ramalho JF, Silva SR, Silva JR.Horse riding (HR) has gain popularity in Portugal, thereby increasing the number of related injuries. This study identifies frequently occurring injuries in Portuguese riders, the conditions under which they occur, and preventive measures. Methods: A retrospective cohort study. Methods: We included 216 Portuguese riders practicing HR at the time of the study with ≥1 year of experience. Data were obtained from a questionnaire that characterized first and second rider injuries; we opted for a systematic method to assess the riders' injuries, in a temporal order. Questions regarding demographi...
Treatment of racehorse superficial digital flexor tendonitis: A comparison of stem cell treatments to controlled exercise rehabilitation in 213 cases.
Equine veterinary journal    January 16, 2023   Volume 55, Issue 6 979-987 doi: 10.1111/evj.13922
Salz RO, Elliott CRB, Zuffa T, Bennet ED, Ahern BJ.Overstrain of the superficial digital flexor tendon (SDFT) is a common Thoroughbred racehorse limb injury requiring treatment. Objective: To determine whether treatment of SDFT lesions in flat Thoroughbred racehorses with autologous bone marrow-derived mesenchymal stem cells (BM-MSCs) or allogenic adipose-derived mesenchymal stem cells (A-MSCs) is associated with improved likelihood of returning to racing, when compared to racehorses managed with a controlled exercise rehabilitation program (CERP) alone. Methods: Retrospective cohort study combining clinical treatment records with race records...
No correlation found between palpation and ultrasound for evaluation of effusion in the medial femorotibial and femoropatellar joint compartments of horses.
Journal of the American Veterinary Medical Association    January 5, 2023   1-5 doi: 10.2460/javma.23.10.0582
Ruff JH, Tufts S, Robertson J, Horne C, Schnabel LV, Jacobs C.To compare palpation and ultrasound scores of effusion of the medial femorotibial and femoropatellar joints of horses. Methods: 40 horses (80 stifles) were evaluated over a 12-week period. Methods: Horses > 1 year of age without history of stifle disease were enrolled from September to December 2022. Palpation of right and left medial femorotibial and femoropatellar joint compartments was performed. Amount of effusion was scored by a board-certified large animal surgeon, a third-year large animal surgery resident, and an equine sports medicine intern. Effusion of right and left medial femoroti...
Equine blood flow restriction training: Safety validation.
Equine veterinary journal    January 4, 2023   Volume 55, Issue 5 872-883 doi: 10.1111/evj.13904
Johnson SA, Frisbie DD, Griffenhagen GM, King MR.Blood flow restriction (BFR) has become a key rehabilitative tool for human orthopaedic conditions. With modernised technology and evolution of clinical application, patient-specific delivery of occlusion percentages is now considered the standard of care in human patients due to improved therapeutic outcomes and minimised safety risks. Safety validation and limb occlusion pressure (LOP) data for horses, however, are lacking. Objective: (1) To determine if BFR exposure resulted in forelimb biomechanical gait dysfunction as safety validation and (2) to investigate inter-horse and inter-limb LOP...
A pilot observational study of horsemanship skills training for veterans with posttraumatic stress disorder.
Complementary therapies in medicine    December 16, 2022   Volume 72 102910 doi: 10.1016/j.ctim.2022.102910
Marchand WR, Smith J, Hoopes KH, Osborne M, Andersen SJ, Bell K, Nazarenko E, Macneill R, Joubert K.Pilot assessment of an equine-assisted services intervention for Veterans with posttraumatic stress disorder. Methods: Prospective cohort. Methods: A large Veterans Administration healthcare system. Methods: Two sessions of instruction in horsemanship skills and two sessions of trail rides. Methods: Safety and feasibility of recruitment, retention, providing the intervention, and measuring outcomes, as well as acceptability of the intervention to the population studied were the main outcome measures. Results: There were no injuries among the 18 participants, 6 staff, or 11 equines over the 3 m...
Equine-assisted services for individuals with substance use disorders: a scoping review.
Substance abuse treatment, prevention, and policy    December 14, 2022   Volume 17, Issue 1 81 doi: 10.1186/s13011-022-00506-x
Diaz L, Gormley MA, Coleman A, Sepanski A, Corley H, Perez A, Litwin AH.The implementation of equine-assisted services (EAS) during treatment for psychological disorders has been frequently documented; however, little is known about the effect of EAS on outcomes for populations with substance use disorder (SUD). The purpose of this scoping review was to synthesize existing literature reporting the effects of EAS when incorporated into SUD treatment. This review followed guidelines in the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) checklist for scoping reviews. A search of four databases (MEDLINE/PubMed, PsycINFO, the Cumulative Ind...
A Case Series of 11 Horses Diagnosed with Bone Spavin Treated with High Intensity Laser Therapy (HILT).
Journal of equine veterinary science    December 2, 2022   Volume 120 104188 doi: 10.1016/j.jevs.2022.104188
Zielińska P, Śniegucka K, Kiełbowicz Z.The aim of this work was to characterize and describe the effect of High Intensity Laser Therapy (HILT) used in the treatment of chronic osteoarthritis in horses. Over a 2 year period, 11 horses with diagnosed bone spavin were treated with HILT as a monotherapy. The horses chosen for this report presented hind limb lameness, were positive in a spavin flexion test and showed improvement after intra-articular anesthesia of the tarsometatarsal joint. Additionally, all the horses presented radiological signs of tarsus osteoarthritis and had not been treated for bone spavin for a minimum of 6 month...
Free Swimming and Exercise-Induced Pulmonary Hemorrhage in Endurance Horses: A Preliminary Study.
Journal of equine veterinary science    December 2, 2022   Volume 120 104182 doi: 10.1016/j.jevs.2022.104182
Vinardell T, David F, Galezowski AM, Ali M, Qasim M, Massie SL, McCrae P, Filho S, Leguillette R.Swimming is used for rehabilitation of musculoskeletal injuries and for conditioning to improve equine fitness. However, there are anecdotal reports that suggest that tethered swimming can induce epistaxis, likely secondary to exercise-induced pulmonary hemorrhage (EIPH). The objective of this observational, prospective study was to investigate if EIPH occurs during intensive free-swimming training sessions (5 × 70m) using 15 endurance horses. On tracheo-bronchoscopic evaluations following swimming, low grade mucus scores were observed, but no tracheal blood was observed. Bronchoalveolar la...
Interaction during equine-facilitated rehabilitation from the rehabilitees’ perspective – A phenomenological study.
Physiotherapy theory and practice    November 27, 2022   1-12 doi: 10.1080/09593985.2022.2151332
Mattila M, Honkanen S, Sjögren T, Piirainen A, Aartolahti E.Interaction has a multidimensional role in equine-facilitated rehabilitation. Unassigned: The aim of this study was to understand rehabilitees' experiences of interaction during equine-facilitated rehabilitation. Unassigned: Six children and four adults with individual diagnoses or reasons to participate in equine-facilitated rehabilitation were included in this qualitative study. Data were collected by interviewing individually the rehabilitees. A phenomenological Spiegelberg's seven-phase meaning analysis was performed to reveal the meanings. Unassigned: Seven meanings were identified: 1) Tr...
Modified toggle pin technique combined with prosthetic capsular reconstruction for surgical stabilization of coxofemoral luxation in a Shetland pony.
Veterinary surgery : VS    November 21, 2022   Volume 52, Issue 2 221-228 doi: 10.1111/vsu.13915
Müller EMT, Ehrle A, Pozzi A, Lasarzik de Ascurra J, Lischer CJ, Kümmerle JM.To describe open reduction and surgical stabilization of a coxofemoral luxation in a pony using a modified toggle pin technique and prosthetic joint capsule reconstruction without osteotomy of the greater trochanter. Methods: A 2-year-old Shetland pony with a bodyweight of 167 kg. Methods: Case report. Methods: Radiographic examination confirmed craniodorsal luxation of the left coxofemoral joint. An open reduction with the aid of a pulley system was performed. A toggle pin was inserted through a bone tunnel extending from the level of the femoral shaft through the femoral head and the cente...
Systematic Review of Complementary and Alternative Veterinary Medicine in Sport and Companion Animals: Extracorporeal Shockwave Therapy.
Animals : an open access journal from MDPI    November 12, 2022   Volume 12, Issue 22 doi: 10.3390/ani12223124
Boström A, Bergh A, Hyytiäinen H, Asplund K.Extracorporeal shockwave therapy (ECSWT) is increasingly used to treat different types of musculoskeletal conditions in sport and companion animals. To explore the scientific basis for the treatment, we conducted a systematic review of the literature on ECSWT used in horses, dogs, and cats. Methods: Relevant articles published in 1980-2020 were identified from three major databases. Each article was assessed for risk of bias. Results: The review identified 27 relevant articles on the effects of ECSWT in horses, nine in dogs, but none in cats. Typically, ECSWT involved one to three treatment se...
Previous Exercise on a Water Treadmill at Different Depths Affects the Accelerometric Pattern Recorded on a Track in Horses.
Animals : an open access journal from MDPI    November 9, 2022   Volume 12, Issue 22 doi: 10.3390/ani12223086
Saitua A, Castejón-Riber C, Requena F, Argüelles D, Calle-González N, de Medina AS, Muñoz A.During a water treadmill (WT) exercise, horses change their accelerometric patterns. We aimed to analyze if these changes persist during terrestrial locomotion. Six horses were randomly subjected to 40 min duration WT exercises, without water (WW), at the depth of fetlock (FET), carpus (CAR) and stifle (STF), with a day off between them. Before and after 30 min after WT, horses were evaluated at walk and at trot on a track with a triaxial accelerometer fixed on the pectoral (PECT) and sacrum (SML) regions. The percent of change from baseline (before WT and after each exercise session) were cal...
Adaptations in equine appendicular muscle activity and movement occur during induced fore- and hindlimb lameness: An electromyographic and kinematic evaluation.
Frontiers in veterinary science    November 8, 2022   Volume 9 989522 doi: 10.3389/fvets.2022.989522
St George LB, Spoormakers TJP, Smit IH, Hobbs SJ, Clayton HM, Roy SH, van Weeren PR, Richards J, Serra Bragança FM.The relationship between lameness-related adaptations in equine appendicular motion and muscle activation is poorly understood and has not been studied objectively. The aim of this study was to compare muscle activity of selected fore- and hindlimb muscles, and movement of the joints they act on, between baseline and induced forelimb (iFL) and hindlimb (iHL) lameness. Three-dimensional kinematic data and surface electromyography (sEMG) data from the fore- (triceps brachii, latissimus dorsi) and hindlimbs (superficial gluteal, biceps femoris, semitendinosus) were bilaterally and synchronously c...
Equine shock wave therapy – where are we now?
Equine veterinary journal    October 28, 2022   Volume 55, Issue 4 593-606 doi: 10.1111/evj.13890
Johnson SA, Richards RB, Frisbie DD, Esselman AM, McClure SR.Over the past three decades, electrohydraulic extracorporeal shock wave therapy (ESWT) as a treatment modality for equine orthopaedic disorders has sparked exponential interest among practitioners, but its clinical applications are quickly evolving and a current review highlighting modernised equine clinical use is lacking. The objective of this review is to summarise the most current ESWT technology, evidence for its use, proposed mechanisms of action and clinical applications in horses while also highlighting the areas requiring further investigation. The three ways to generate a shock wave ...
Clinical and imaging findings, treatment details and outcomes in foals with extensor tendon rupture-A multicentre retrospective study.
Equine veterinary journal    October 28, 2022   Volume 55, Issue 5 777-787 doi: 10.1111/evj.13893
Mokry A, Bernhard C, Faulkner J, Colgate VA, Dumoulin M, Pille F, Vanderperren K, Delling U, Oosterlinck M.Forelimb extensor tendon rupture (ETR) in foals is sparsely documented. Objective: To describe clinical and imaging findings, treatment details, and long-term outcome in foals with ETR. Methods: Multicentre retrospective case-series. Methods: Clinical record review of foals with ETR, presented to Faculty of Veterinary Medicine at Ghent University (Belgium), the Clinic for Horses at the University of Veterinary Medicine Hannover (Germany), and Rossdales Equine Hospital (UK) between 2009-2021. Long-term outcome based on structured telephone interview with the owner at the university hospitals an...
The Effect of Chiropractic Treatment on Limb Lameness and Concurrent Axial Skeleton Pain and Dysfunction in Horses.
Animals : an open access journal from MDPI    October 19, 2022   Volume 12, Issue 20 doi: 10.3390/ani12202845
Maldonado MD, Parkinson SD, Story MR, Haussler KK.Chiropractic care is a common treatment modality used in equine practice to manage back pain and stiffness but has limited evidence for treating lameness. The objective of this blinded, controlled clinical trial was to evaluate the effect of chiropractic treatment on chronic lameness and concurrent axial skeleton pain and dysfunction. Two groups of horses with multiple limb lameness (polo) or isolated hind limb lameness (Quarter Horses) were enrolled. Outcome measures included subjective and objective measures of lameness, spinal pain and stiffness, epaxial muscle hypertonicity, and mechanical...
Treatment of a Mandibular Diastemal Fracture Using Locking Compression Plate and Cerclage Wire in a Mare.
Journal of veterinary dentistry    October 18, 2022   Volume 40, Issue 1 75-80 doi: 10.1177/08987564221129986
Godoy VM, Oliveira NFOE, Paretsis NF, Silva SCD, Souza AF, De Zoppa ALDV, Corrêa RR.This report describes the combination of two surgical fracture repair techniques and the postoperative management of a mandibular diastemal fracture in a two-year-old mare. The mare was referred to a veterinary hospital with a laceration over the body of the right mandible. Radiographic assessment revealed two mesial fracture lines involving the second premolar tooth and a ventrally displaced bone fragment. The mare was treated under general anesthesia and the fracture was corrected using open reduction and plate fixation. A 3.5 mm narrow 15-hole locking compression plate with seven locking ...
Rehabilitation: Proprioception, Incoordination, and Paresis.
The Veterinary clinics of North America. Equine practice    October 14, 2022   Volume 38, Issue 3 557-568 doi: 10.1016/j.cveq.2022.06.010
King MR.Physiotherapeutic exercises aimed at stimulating motor control, flexibility, and stability are regularly employed in human physical therapy programs. Specifically, the use of such exercises has been shown to reduce both pain and reinjury. Pursuant to the equine patient, several core strengthening exercises and their role in activating deep epaxial musculature to subsequently improve postural motor control and alter thoracolumbar kinematics have been investigated. Both baited and passive exercises offer opportunities to facilitate stretching during dynamic phases and strengthening during static...
Spinal Mobilization and Manipulation in Horses.
The Veterinary clinics of North America. Equine practice    October 13, 2022   Volume 38, Issue 3 509-523 doi: 10.1016/j.cveq.2022.06.008
Haussler KK, Holt TN.There is a growing body of evidence to support the use of spinal mobilization and manipulation techniques in equine practice. Outcome parameters reported across studies include measures of joint motion, nociception, muscle tone, and performance. Spinal examination procedures include static and dynamic assessments of the quantity and the quality of both active and passive movements. Tiered treatment approaches are recommended to stage the application of various therapies based on ease, cost, and efficacy.
Clinical Application of Myofascial Therapy in Horses.
The Veterinary clinics of North America. Equine practice    October 13, 2022   Volume 38, Issue 3 493-507 doi: 10.1016/j.cveq.2022.06.007
Luomala T.Fascia is a complex and intriguing tissue, which can take on structural properties of being loose or dense, irregular or regular. Fascia functions by connecting, separating, and uniting different structures of the body. Myofascial dysfunction can be a significant source of pain and can be categorized as densification, adhesion, and fibrosis. Digital palpation and treatment of myofascial disorders can be provided via superficial or deep techniques. Different myofascial treatment techniques include slow and fast techniques, which can be applied at different depths, angles, and rhythms.
Therapeutic Exercises for Equine Sacroiliac Joint Pain and Dysfunction.
The Veterinary clinics of North America. Equine practice    October 13, 2022   Volume 38, Issue 3 569-584 doi: 10.1016/j.cveq.2022.07.002
Goff L.This article provides the equine practitioner with a review of sacroiliac joint pain and dysfunction and outlines the importance of providing a specific prescription for a safe and effective therapeutic exercise program. The continuum of clinical dysfunction associated with the sacroiliac region is presented with prescribed interventions. The intent is to encourage the practitioner to perform a thorough assessment of the sacroiliac joint and the adjacent soft tissues and to use sound clinical reasoning to formulate a therapeutic exercise plan.
Diagnosis and outcome following tenoscopic surgery of the digital flexor tendon sheath in German sports and pleasure horses.
Equine veterinary journal    October 11, 2022   Volume 55, Issue 1 48-58 doi: 10.1111/evj.13856
Cender AN, Mählmann K, Ehrle A, Merle R, Pieper L, Lischer CJ.Digital flexor tendon sheath (DFTS) pathology is an important cause of lameness in horses. The outcome after surgical treatment is variable and depends on the exact diagnosis. Objective: To (1) describe the prevalence of lesions associated with lameness caused by nonseptic DFTS tenosynovitis in a large population of German sports and pleasure horses; (2) determine the sensitivity and specificity of diagnostic imaging techniques for identifying lesions within the DFTS with tenoscopic diagnosis being the gold standard; (3) explore associations between tenoscopically diagnosed lesions and signalm...
Metabolomic Profiles in Starved Light Breed Horses during the Refeeding Process.
Animals : an open access journal from MDPI    September 21, 2022   Volume 12, Issue 19 2527 doi: 10.3390/ani12192527
Main SC, Brown LP, Melvin KR, Campagna SR, Voy BH, Castro HF, Strickland LG, Hines MT, Jacobs RD, Gordon ME, Ivey JLZ.The large population of emaciated horses continues to be an issue troubling the equine industry. However, little is known regarding the collection of equine metabolites (metabolome) during a malnourished state and the changes that occur throughout nutritional rehabilitation. In this study, ten emaciated horses underwent a refeeding process, during which blood samples were collected for a blood chemistry panel and metabolomics analysis via ultrahigh performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS). Significant differences among blood chemistry analytes and metabo...