Analyze Diet

Topic:Statistical Analysis

Statistical analysis in equine research involves the application of mathematical techniques to collect, review, and interpret data related to horse health, behavior, and performance. This field encompasses a variety of methods, including descriptive statistics, inferential statistics, and multivariate analysis, to identify patterns, test hypotheses, and draw conclusions from equine data. Statistical analysis aids in understanding various aspects of equine science, such as disease prevalence, treatment efficacy, genetic traits, and performance metrics. This page aggregates peer-reviewed research studies and scholarly articles that explore the methodologies, applications, and outcomes of statistical analysis in the context of equine studies.
Evaluating Forelimb and Hindlimb Joint Conformation of Morna Racehorses (Equus caballus).
Veterinary sciences    January 5, 2025   Volume 12, Issue 1 doi: 10.3390/vetsci12010020
Ahmad I, Ijaz S, Usman MM, Safdar A, Khan IU, Zeeshan M, Bukhari SSUH.Measuring limb joint angles is crucial for understanding horse conformation, performance, injury diagnosis, and prevention. While Thoroughbred horses have been extensively studied, local Pakistani breeds (e.g., Morna racehorse) have not received scientific attention. This study aimed to quantify normal angles of limb joints in the Morna breed. Limb joint angles of standing horses (n = 50) were quantified using a measuring tape, height stick, protractor scale, and goniometer. The mean and standard deviation (Mean ± SD) values for the forelimb joint angles were 123.02 ± 3.46° for elbow, 171.5...
Single-Step Genome-Wide Association Study of Factors for Evaluated and Linearly Scored Traits in Swedish Warmblood Horses.
Journal of animal breeding and genetics = Zeitschrift fur Tierzuchtung und Zuchtungsbiologie    January 4, 2025   Volume 142, Issue 5 499-512 doi: 10.1111/jbg.12923
Nazari-Ghadikolaei A, Fikse WF, Viklund ÅG, Mikko S, Eriksson S.Swedish Warmblood horses (SWB) are bred for show jumping and/or dressage with young horse test scores as indicator traits. This study aimed to investigate possible candidate genes and regions of importance for evaluated and linearly scored young horse test traits. A single-step genome-wide association study (ssGWAS) was done using the BLUPF90 suite of programs for factors scores from factor analysis of traits assessed at young horse tests together with height at withers. The ssGWAS included 20,814 SWB with factors scores for four factors for evaluated traits. A total of 6436 of these horses al...
Real-time transrectal ultrasonographic measurement of the fetal eye (vitreous body) to predict parturition in bucking horse mares.
Journal of equine veterinary science    January 3, 2025   Volume 145 105345 doi: 10.1016/j.jevs.2025.105345
Gonzalez SW, Espy BMK, Stefanovski D, Turner RM.Monitoring gestation and predicting parturition in mares with unknown breeding dates is challenging, especially in pasture bred herds of bucking horses. Transrectal ultrasonographic measurement of fetal vitreous body length is a validated predictor of foaling dates, but previous research suggested a need for breed-specific parameters to develop accurate indices for respective breeds. This study aimed to develop a scale associating fetal vitreous body length with days before parturition (DBP) in bucking horses. Biannual data collection over 2 years identified 199 pregnancies out of 240 reproduc...
Assessing the benzimidazole resistance in equine strongyles by in vitro methods.
Veterinary parasitology    January 3, 2025   Volume 334 110387 doi: 10.1016/j.vetpar.2024.110387
Königová A, Babják M, Kuzmina TA, Burcáková Ľ, Syrota Y, Várady M.The study aimed to conduct a survey on the occurrence of benzimidazole (BZ) resistance in strongyles by in vitro egg hatch test (EHT) and larval development test (LDT) and to identify the effective indicators of early resistance detection on horse farms with associated risk factors analysis appraisal. In total, 203 horses from 8 farms underwent the fecal sampling of which 77 horses were selected for in vitro testing. Simultaneously, 18 horses were chosen to analyse the results of in vitro tests compared to the in vivo fecal egg count reduction test (FECRT). The EHT indicated the presence of re...
Review of biological variation and its applications in interpretation of equine clinical pathology results.
Equine veterinary journal    January 2, 2025   doi: 10.1111/evj.14454
Fernandes T, Robin M, Freeman KP.Biological variation in laboratory results refers to physiological fluctuations that occur around a homeostatic setpoint (HSP) for various laboratory measurands. Assessment of biological variation includes determining individual variation (CV), group variation (CV), and analytical variation (CV). Reference change value (RCV) is an objective tool for an evidence-based approach to interpret data by assessing the significance of consecutive results in an individual for the diagnosis, prognosis, and monitoring of disease. The individualised reference intervals (iRI) are derived from the determinat...
Soft palate angle and basihyoid depth increase with tongue size and with body condition score in horses.
Equine veterinary journal    January 2, 2025   doi: 10.1111/evj.14445
Talbot AM, Shanks-Boon H, Baldwin CM, Barnes H, Maddox TW.Obesity has been associated with human obstructive sleep apnoea and canine brachycephalic obstructive airway syndrome. The effect of body condition score (BCS) on structures of the oropharynx, nasopharynx and upper airway of the horse has not been investigated. Objective: To investigate the effect of BCS on tongue measurements, soft palate angle and basihyoid depth in horses. Methods: Retrospective, analytical, cross-sectional. Methods: Computed tomographic (CT) images of the head of 58 horses were assessed. DICOM viewing software was used to measure head length, basihyoid-skin depth, soft pal...
Radiographic Morphometric Measurements of the Donkey’s Distal Hind Limb.
Animals : an open access journal from MDPI    December 25, 2024   Volume 15, Issue 1 doi: 10.3390/ani15010022
Schaus K, Wacker J, Jandowsky A, Büttner K, Röcken M, Bartmann CP.Morphometric measurements of the toe serve as a reference for the objective assessment of the presence of a pathological condition and as a guide for the correct trimming of the hooves. Therefore, the anatomical differences between horses and donkeys oblige an autonomous database with reference values for the donkey. The aim of this study was therefore to provide readings of the distal hind limb on radiographs of donkeys. Radiographs of the distal hind limb in two projections of 41 generally sound donkeys without lameness in walk were taken after previous hoof trimming and preparation accordin...
Genomic-Inbreeding Landscape and Selection Signatures in the Polo Argentino Horse Breed.
International journal of molecular sciences    December 24, 2024   Volume 26, Issue 1 26 doi: 10.3390/ijms26010026
Azcona F, Molina A, Demyda-Peyrás S.Analyzing genetic variability and inbreeding trends is essential for effective breed management in animal populations. To this, the characterization of runs of homozygosity (ROH) provides a good genomic approach to study the phenomena. The Polo Argentino (PA) breed, globally recognized as the best adapted to playing polo, is known for its strong influence of Thoroughbreds, intense selective breeding, and extensive use of reproductive biotechnologies. This study investigates the PA's genomic variability, by characterizing the ROH landscape and identifying ROH islands (ROHi) as potential genomic...
Gestational length of Thoroughbred mares kept in tropical and subtropical climates.
Theriogenology    December 21, 2024   Volume 234 192-197 doi: 10.1016/j.theriogenology.2024.12.017
Silva GC, Nogueira CEW, Scalco R, Pizzi GLBL, Leite TA, Bastos R, Curcio BR.Gestation length (GL) in horses varies widely, influenced by multiple variables, including maternal, fetal, and environmental factors. This study aimed to investigate and quantify the relative contributions of climatic (photoperiod and temperature-humidity index - [THI]), maternal (age and parity), fetal (sex) and environmental (year and month of foaling) variables influencing gestation length in Thoroughbred mares. Retrospective data encompassing 704 pregnancies across nine breeding seasons in tropical and subtropical Brazilian climates were analyzed. Stepwise regression analysis identified f...
Anticipatory Behaviour During the Approach to Feeding Times as a Measure of Horse Welfare.
Animals : an open access journal from MDPI    December 20, 2024   Volume 14, Issue 24 3677 doi: 10.3390/ani14243677
Mata F, Boyton G, Young T.Anticipatory behaviour is increasingly being recognised as a measure of animal welfare. This behaviour is linked to reward sensitivity, which reflects the balance of positive and negative experiences. This study examined anticipatory behaviour in horses fed either ad libitum or rationed diets, aiming to identify differences in behaviour patterns during the periods of one hour immediately before and after feeding. Behavioural data were collected via video surveillance over five days, focusing on the pre- and post-feeding periods of stabled horses. The data were successfully fit to Poisson model...
Bayesian Recursive and Structural Equation Models to Infer Causal Links Among Gait Visual Scores on Campolina Horses. Bussiman F, Richter J, Hidalgo J, Silva FFE, Ventura RV, Carvalho RSB, Mattos EC, Ferraz JBS, Eler JP, de Carvalho Balieiro JC.Gait visual scores are widely applied to horse breeding because they are a fast and easy phenotyping strategy, allowing the numeric interpretation of a complex biological process such as gait quality. However, they may suffer from subjectivity or high environmental influence. We aimed to investigate potential causal relationships among six visual gait scores in Campolina horses. The data included 5475 horses with records for at least one of the following traits: Dissociation (Di), Comfort (C), Style (S), Regularity (R), Development (De), and Gait total Scores (GtS). The pedigree comprised thre...
Factors affecting survival of foals with pneumonia in a referral hospital.
BMC veterinary research    December 18, 2024   Volume 20, Issue 1 562 doi: 10.1186/s12917-024-04405-0
Rossi HS, Hyytiäinen HK, Junnila JJT, Rajamäki MM, Mykkänen AK.Pneumonia is a common condition in ailing neonatal foals, and it remains an important cause of morbidity and mortality in this veterinary patient group. Factors affecting the survival of young foals with pneumonia have not been thoroughly investigated. The aim of this study was to explore the potential prognostic factors associated with survival of these foals. Fifty foals under one month of age with pneumonia were included in this retrospective clinical study. The foals were divided into groups based on survival (survived to discharge or died/euthanised during hospitalisation). Multiple clini...
Rising trends of inbreeding in Japanese Thoroughbred horses.
Journal of equine science    December 12, 2024   Volume 35, Issue 4 57-61 doi: 10.1294/jes.35.57
Watanabe M, Sato F, Innan H.We investigated the changes in inbreeding levels in Japanese Thoroughbred horses over the past 46 years. Our results show a significant increase in inbreeding over the past 30 years, primarily due to the influence of two sires, Northern Dancer and Sunday Silence. Northern Dancer's bloodline spread gradually through descendants like Northern Taste, leading to a slower increase in the inbreeding coefficient. In contrast, Sunday Silence was directly imported and became a leading sire, causing a rapid increase in his blood proportion and inbreeding coefficient. Our findings suggest that monitoring...
Risk factors for fatality in jump racing Thoroughbreds in Great Britain (2010-2023).
Equine veterinary journal    December 12, 2024   Volume 57, Issue 4 870-877 doi: 10.1111/evj.14450
Allen SE, Taylor S, Given J, Verheyen KL.The British horseracing industry is committed to reducing equine fatalities in jump racing. Race-related fatalities are a major welfare concern and threaten the sport's social licence to operate. Objective: To describe the risk of, and determine risk factors for, fatality in British jump racing. Methods: Retrospective cohort. Methods: Analyses included all starts made in British jump races between January 2010 and April 2023. Available information for each horse, race, racecourse, trainer and jockey was collated and combined with details of all fatalities recorded by official veterinary office...
Genetic composition of Kazakh horses of Zhabe type evaluated by SNP genotyping.
Heliyon    December 12, 2024   Volume 11, Issue 1 e41173 doi: 10.1016/j.heliyon.2024.e41173
Pozharskiy A, Beishova I, Nametov A, Shamshidin A, Ulyanova T, Kovalchuk A, Ulyanov V, Shamekova M, Bekova G, Gritsenko D.Horses are animals traditionally playing prominent role as both food source and working animals for Kazakh people. Zhabe horses are traditional type of indigenous Kazakh horses characterized by versatility and adaptation to conditions of Central Asia. The present work focuses on examination of genetic structure of Zhabe horses using SNP genotyping with addition of previously published data. Total 1038 individuals including 403 new samples of Zhabe horses and 42 sample of white horses 'Zhetysu Asyly' have been considered. DNA was extracted from hair roots using commercial DNA isolation kit and ...
Y-chromosomal insights into the breeding history and sire line genealogies of two traditional Baroque horse breeds: Lipizzaner and Kladruber.
Journal of equine veterinary science    December 4, 2024   Volume 144 105252 doi: 10.1016/j.jevs.2024.105252
Radovic L, Remer V, Rigler D, Felkel S, Brem G, Wallner B.The paternally inherited, male-specific part of the Y chromosome (MSY) is an ideal marker for studying the origin, genealogies, and historical connections of horse patrilines. Here, we performed fine-scaled MSY haplotype (HT) analysis in two Baroque horse breeds, the Lipizzaner and the Kladruber, both known for their long-standing tradition of sire line breeding and interconnected genealogies. We genotyped 95 MSY markers using KASP technology in 90 stallions representing all patrilines of both breeds. We identified 14 HTs across eight Lipizzaner sire lines and six HTs in eight Kladruber lines....
Quantitative lameness assessment in horses by using an accelerometer-based simple device: A preliminary study.
Open veterinary journal    November 30, 2024   Volume 14, Issue 11 3089-3099 doi: 10.5455/OVJ.2024.v14.i11.38
Crecan CM, Ciulu-Angelescu V, Morar IA, Lupșan AF, Tripon MA, Tripon MA, Bungărdean D, Daradics Z, Peștean CP.Global technological advancements have shifted equine lameness diagnostics from purely subjective assessment toward more objective, quantitative methods. Traditional gait evaluations are increasingly being supplemented by innovative technologies to enhance diagnostic accuracy. Unassigned: This study aimed to determine if traditional gait assessment could be effectively complemented by quantitative measurements using an affordable, self-constructed device, the Lameness Detector 0.1, which incorporates inertial motion sensors. Unassigned: A total of 42 adult sport horses diagnosed with hindleg l...
Injury Patterns and Associated Demographic Characteristics in Children with a Fracture from Equines: A US National Based Study.
Children (Basel, Switzerland)    November 27, 2024   Volume 11, Issue 12 1443 doi: 10.3390/children11121443
Loder RT, Walker AL, Blakemore LC.Fractures often occur due to equestrian activities with injury patterns varying by age. The purpose of this study was to investigate in detail fracture patterns and associated demographics in children due to equine activities. Methods: The US National Electronic Injury Surveillance System was queried for all injuries with the consumer product code 1239 (horseback riding) from 2000 to 2023. Those <16 years old with fractures were extracted. Statistical analyses were performed with SUDAAN 11.0.01™ software to obtain national estimates. Results: There were an estimated 101,677 patients with a f...
Oocyte holding and in vitro maturation duration between 28 and 34 hours do not affect equine OPU-ICSI outcomes.
Theriogenology    November 22, 2024   Volume 233 64-69 doi: 10.1016/j.theriogenology.2024.11.019
Broothaers K, Pascottini OB, Hedia M, Angel-Velez D, De Coster T, Peere S, Polfliet E, Van den Branden E, Govaere J, Van Soom A, Smits K.Previous studies in the horse highlight the potential benefit of prolonged in vitro maturation (IVM) (34 h) compared to short IVM (24 h) with or without prior oocyte holding, but little is known about the optimal IVM duration within this interval. To determine the effect of oocyte holding and duration of IVM ranged between 28 and 34 h on nuclear maturation, cleavage, blastocyst formation, and pregnancy rates, a retrospective study was performed in an equine clinical OPU-ICSI setting. The study included data of 2114 aspirated oocytes from 201 OPU-ICSI sessions. Duration of IVM was divided in...
Evaluation of digital radiographic measurements for the diagnosis of acute laminitis.
Equine veterinary journal    November 20, 2024   Volume 57, Issue 4 931-942 doi: 10.1111/evj.14436
Skelton G, Acutt E, Stefanovski D, van Eps A.Traditional radiographic measurements of distal phalanx (Pd) displacement based on the outer hoof wall are not useful for diagnosis of acute laminitis. Objective: We hypothesised that the distance between the inner hoof wall and Pd ('lamellar lucent zone'; LLZ) measured on lateromedial digital radiographs would be increased in horses with acute and subacute laminitis compared with healthy horses. Methods: Retrospective cohort study and in vivo experimental study. Methods: Forelimb radiographs from 32 healthy and 18 laminitic mixed-breed horses were analysed retrospectively. Laminitis was defin...
Estimation of Genetic Parameters for Racing Time and Ranking in Thoroughbred Horses.
Journal of equine veterinary science    November 18, 2024   105244 doi: 10.1016/j.jevs.2024.105244
Padilha DAO, Padilha SF, Martins R, Scheffer BEM, Miliorini MR, Dias LT, Teixeira RA.This study aimed to estimate genetic parameters for race time in seconds and final ranking, as well as to analyze the genetic trends associated with race time. The study utilized a dataset consisting of 23,290 records of race times and final ranks at distances of 1,000, 1,600, and 2,000 m from 6,213 Thoroughbred horses from the São Paulo Jockey Club. Our model considered the year of the run, animal sex, race class, track conditions, the linear effect of horse weight and age, and the quadratic effect of age as fixed covariates. Random effects included direct additive genetic, animal permanent ...
National survey reveals elastic price sensitivity for select equine veterinary services.
Journal of the American Veterinary Medical Association    November 13, 2024   1-5 doi: 10.2460/javma.24.09.0576
Gibson OL, Zhao S, Adam E, Stowe CJ.The objective of this study was to estimate the price elasticity of demand for 3 common equine veterinary services: vaccinations (a routine service), lameness examinations (an elective service), and emergency colic surgery (an urgent service). Methods: Data were collected via a nationwide online survey of horse owners from August 15 to September 11, 2023, eliciting their willingness to pay for each service. The link to the online survey was distributed through participating organizations' social media and email lists to the target audience of US residents aged ≥ 18 years who were financially...
Quantifying head and withers movement asymmetry in sound and naturally forelimb lame horses trotting on a circle on hard and soft surfaces.
Equine veterinary journal    November 12, 2024   Volume 57, Issue 5 1219-1230 doi: 10.1111/evj.14430
Taddey CM, Roecken M, Kreling KM, Cruz AM.Reliable lameness thresholds for vertical head displacement for trotting on a circular path and on different surfaces have yet to be defined. Withers movement in lame horses could help improve the diagnostic accuracy of sensor technology. Objective: To define head movement lameness threshold ranges and describe the relationship between head and withers movement during trotting under different circumstances in sound and forelimb lame horses. Methods: Retrospective analysis of clinical data and comparison with control group. Methods: Fifty-five sound and thirty-four naturally lame horses were tr...
A genome-wide association study of the racing performance traits in Yili horses based on Blink and FarmCPU models.
Scientific reports    November 12, 2024   Volume 14, Issue 1 27648 doi: 10.1038/s41598-024-79014-w
Wang C, Zeng Y, Wang J, Wang T, Li X, Shen Z, Meng J, Yao X.Racing performance traits are the main indicators for evaluating the performance and value of sport horses. The aim of this study was to identify the key genes for racing performance traits in Yili horses by performing a genome-wide association study (GWAS). Breeding values for racing performance traits were calculated for Yili horses (n = 827) using an animal model. Genome-wide association analysis of racing performance traits in horses (n = 236) was carried out using the Blink, and FarmCPU models in GAPIT software, and genes within the significant regions were functionally annotated....
Reproducibility and agreement of radiographic assessment of carpal deformities in foals.
Frontiers in veterinary science    November 7, 2024   Volume 11 1479790 doi: 10.3389/fvets.2024.1479790
Charles A, Peeters X, Verbrugghe C, Vandersmissen M, Evrard L, Busoni V.The Pivot Point (PP) method is commonly used in the radiographic assessment of carpal deformities in young foals, as the range of deviation may influence treatment choice. The aims of this study were to assess the intra- and interobserver reproducibility of the PP method and subjective radiographic evaluation without line drawing to establish the anatomical site responsible for carpal deviation in foals and to evaluate the agreement between these two techniques. Unassigned: Anonymized radiographs of foals presented for investigation of carpal deformity or prematurity were retrospectively and i...
Comparison of a customized glycemic pellets challenge with the oral sugar test to measure glycemic and insulinemic responses in horses.
Journal of veterinary internal medicine    October 28, 2024   Volume 38, Issue 6 3281-3287 doi: 10.1111/jvim.17191
Thane K, Sonntag J, Warnken T, Reiche D, Uricchio C, Frank N.Testing for insulin dysregulation (ID) in horses is commonly performed to guide management and therapeutic strategies. Objective: To evaluate a newly developed glycemic pellets challenge (GPC) and compare results to those obtained using the low-dose oral sugar test (OST). Methods: Twenty-four adult horses with unknown insulin status. Methods: A randomized crossover trial was performed. Horses underwent GPC (0.5 g glycemic carbohydrates/kg body weight) and OST (0.15 mL corn syrup/kg body weight) 7 days apart. Feed was withheld before testing and blood samples were collected at T0, T60, T1...
The Memory Abilities of the Elderly Horse.
Animals : an open access journal from MDPI    October 25, 2024   Volume 14, Issue 21 3073 doi: 10.3390/ani14213073
Studies on short- and long-term memory and their decline with aging in horses are still limited. This research aimed to assess the learning and short- and long-term memory abilities of adult and senior horses. A total of 21 adult horses (5-15 years) and 23 senior horses (16 years and older) were subjected to a behavioral test (Target Touch Test), which was repeated three times each for the initial test (T1) and again after 10 days (T10). Statistical analysis revealed no significant differences between adult and senior horses in the three tests conducted at T1. However, a significant difference...
Advancing equine genomics: the development of a high density Axiom_Ashwa SNP chip for Indian horses and ponies.
Functional & integrative genomics    October 23, 2024   Volume 24, Issue 6 195 doi: 10.1007/s10142-024-01482-0
Ahlawat S, Niranjan SK, Arora R, Vijh RK, Kumar A, Sharma U, Raheja M, Popli K, Yadav S, Mehta SC.The unique horse and pony breeds of India are declining at an alarming rate. These horses have been integral to the Indian culture and customs for centuries and represent a valuable genetic resource. It is imperative to harness the potential of this equine genetic resource that urgently needs conservation. The study highlights the design and development of a high density SNP array, the Axiom_Ashwa to aid in the genetic analysis and conservation efforts for Indian horse and pony breeds. With 613,950 SNPs, this chip offers extensive genome coverage having an average inter-marker distance of 4 k...
Assessment of horse breeding and husbandry practices in southwest Ethiopia: Its implication to design breeding program.
Heliyon    October 18, 2024   Volume 10, Issue 20 e39280 doi: 10.1016/j.heliyon.2024.e39280
Mustefa A, Engdawork A, Sinke S.Ethiopian horses are multipurpose horses that have socioeconomic significance for smallholder farmers. However, studies regarding their husbandry practices have received little attention. Thus, the current study was conducted to assess the horse husbandry practices in the southwest Ethiopia. Data were collected through semi-structured questionnaires from a total of 196 randomly selected respondents. The general linear model and frequency procedures of the Statistical Analysis System (SAS 9.0) were used to analyze the quantitative and qualitative data, while indices were calculated using Micros...
Inertial measurement unit technology for gait detection: a comprehensive evaluation of gait traits in two Italian horse breeds.
Frontiers in veterinary science    October 16, 2024   Volume 11 1459553 doi: 10.3389/fvets.2024.1459553
Asti V, Ablondi M, Molle A, Zanotti A, Vasini M, Sabbioni A.The shift of the horse breeding sector from agricultural to leisure and sports purposes led to a decrease in local breeds' population size due to the loss of their original breeding purposes. Most of the Italian breeds must adapt to modern market demands, and gait traits are suitable phenotypes to help this process. Inertial measurement unit (IMU) technology can be used to objectively assess them. This work aims to investigate on IMU recorded data (i) the influence of environmental factors and biometric measurements, (ii) their repeatability, (iii) the correlation with judge evaluations, and (...