Veterinary procedures in horses encompass a range of medical and surgical interventions performed to diagnose, treat, and manage equine health conditions. These procedures are designed to address various health issues, from routine care to emergency interventions. Common veterinary procedures include vaccinations, dental care, lameness evaluations, reproductive services, and surgical interventions such as colic surgery or fracture repair. This page compiles peer-reviewed research studies and scholarly articles that explore the methodologies, outcomes, and advancements in veterinary procedures for horses, providing insights into their application and efficacy in equine medicine.
Letourneau AR, Butler BA, Martinez PS, da Costa Martins B, Monk CS, Plummer CE.To describe a medial canthoplasty surgical procedure in two horses for correction of euryblepharon, secondary to microphthalmia, and following excision of retrobulbar fat prolapse. Methods: A 1 year old thoroughbred mare was presented for small globes and chronic ocular discharge bilaterally. Ophthalmic examination revealed microphthalmos, euryblepharon, medial canthal pocket syndrome, and dacryocystitis bilaterally with no visual impairments. A 2 year old Appaloosa gelding was presented for evaluation of a smooth, subconjunctival mass anterior to the right globe in the medial canthus. Oph...
Slavik K, Underwood C, Lowndes C, Skelton G, van Eps A.To examine the effects of botulinum toxin inoculation into the deep digital flexor (DDF) muscle on foot biomechanics. Unassigned: 6 healthy horses were injected with botulinum toxin in the DDF of 1 forelimb. The opposite forelimb was untreated. Ground reaction forces (GRF) were measured using a pressure sensor in regions of interest, including the dorsal hoof wall (toe). The location of the center of pressure (COP) was measured relative to the dorsal hoof wall during static standing and at peak stance during walking. Repeat measurements 3, 7, 14, 28, and 42 days after injection were compared t...
Mendes RP, Gonzaga MS, Matheus MM, Bittar MJ, Doria RGS, Corrêa RR.Developmental and eruption abnormalities are common and can result in progressive dental diseases. Ectopic eruption of the affected tooth may be the result of these processes. This study aims to report the occurrence of a permanent mandibular tooth in a horizontal position, with retention of the corresponding deciduous tooth, in a miniature horse. A 2-year-old female miniature horse was admitted to the veterinary hospital with a hard swelling on the left mandibular ramus, which had been growing continuously for three months. After a complete evaluation, it was concluded that the swelling was t...
Çortu A, Çınar H.Rectal examination is routine in equine reproduction but may trigger acute stress affecting welfare and safety. Intraocular pressure (IOP) responds rapidly to autonomic changes and may capture short-lived stress responses. Objective: To evaluate whether rectal examination acutely affects IOP and behavioral responses in mares, and whether reactions differ by reproductive status. Methods: Twenty Rahvan mares (10 pregnant, 30-40 days' gestation; 10 non-pregnant; 4-7 years) underwent transrectal examination without sedation. IOP was measured bilaterally at six time points: 2 min pre-exam (T1), dur...
Imai S, Sato R, Fujiwara R, Terui S, Kimura A, Ichijo T, Kasashima Y, Miyazaki T.A 15-year-old Thoroughbred gelding was presented for examination due to chronic corneal opacity in the right eye, which had been noted for several years. An ophthalmic evaluation revealed dense, irregular corneal opacity; an absence of the direct pupillary light reflex; and a positive dazzle reflex. Fluorescein staining was negative and cytology of a corneal conjunctival swab identified nucleated squamous epithelial cells, but no inflammatory cells. Slit-lamp examination failed to visualize intraocular structure, and the lesion was deemed to be chronic and irreversible. An obstacle-avoidance t...
Mählmann K, Lischer CJ.This technical note describes the use of a manual graphic roadmap (MGR) to assist fluoroscopic guidance during transarterial coil embolization (TACE) for guttural pouch (GP) arterial hemorrhage. Horses underwent MGR-assisted TACE at the Equine Clinic, Free University of Berlin, between 2012 and 2024. Surgery was performed under general anesthesia with fluoroscopic guidance. Following the initial angiogram, a transparent foil was secured to the fluoroscopy monitor, and an assistant traced the vascular anatomy with a waterproof marker to create the MGR, which then served as a guide for catheter ...
Aimonetti E, Genton M, Lischer CJ, Rossignol F.To determine the benefit of using a three-dimensional (3D)-printed titanium aiming device for placing two 5.5-mm transarticular screws during proximal interphalangeal joint (PIPJ) arthrodesis in equine species. Methods: Ex vivo study. Methods: A total of 40 cadaveric forelimbs from adult horses. Methods: A senior surgeon and a first-year resident each performed 20 constructs: 10 using the device and 10 using a freehand technique. Procedure duration and number of intraoperative radiographs were recorded. Final radiographs were evaluated by an ECVS-certified surgeon unaware of treatment group, b...
Strugava L, Serighelli-Júnior G, Stadler LM, Flores LADS, Minozzo GA, Dornbusch LPTC, Moreno JCD, Dornbusch PT.Rectal amputation is a surgical approach indicated for the management of severe rectal lacerations or extensive rectal prolapse when transmural compromise or primary repair is unfeasible. These conditions, although uncommon, present substantial challenges in equine practice due to the anatomical complexity of the pelvic region and the potential for life-threatening complications if not adequately addressed. This study aimed to describe and standardize a reproducible surgical technique for rectal amputation in horse cadavers. Six equine cadavers (mean body mass, 441.83± 66.84 kg) were position...
Cerullo A, Scilimati N, Di Nicola MR, Colla L, Mazza S, Bertoletti A, Nannarone S, Gialletti R.Left dorsal displacement of the large colon (LDDLC) is a common cause of colic in horses and it is frequently associated with recurrent episodes, with significant implications for horse management, athletic performance, and owner satisfaction. Laparoscopic nephrosplenic space (NSS) ablation is a preventive surgical technique aimed at reducing recurrence in horses with a history of medically or surgically treated LDDLC. This retrospective study evaluated 48 horses that underwent laparoscopic NSS ablation in two Italian Veterinary Teaching Hospitals between 2016 and 2024. Short-term outcomes, in...
Bramall LM, Hinnigan GJ, Wattam AD, Owen KR.This case report describes the successful hemithyroidectomy of the largest equine solid follicular adenoma described in the veterinary literature to date. A 19-year-old thoroughbred mare was referred to Oakhill Veterinary Centre for the surgical removal of a large, left-sided thyroid gland measuring 26cm in diameter. Pre-operative considerations, the hemithyroidectomy surgical technique as well as the intra- and post-operative complications encountered, are discussed. Following surgery the horse had no long-term complications and continues to thrive four and a half years post-operatively.
Márquez-Moya A, Carreras-Vico N, Sala-Ayala L, MartÃnez-Bovà R, Cuervo-Arango J.Transvaginal ultrasound-guided follicular aspiration or ovum pick-up (OPU) has become the standard technique for oocyte collection in mares for intracytoplasmic sperm injection (ICSI). Although repeated follicular flushes and wall scraping are commonly used to improve oocyte recovery rate (ORR), the relative contribution of each remains unclear. This study aimed to compare the effects of multiple flushes versus controlled scraping time on ORR in mares. A controlled trial was conducted in two phases: (1) an ex vivo phase using slaughterhouse ovaries (n = 32), and (2) an in vivo phase in c...
Faugier C, Snyder LBC, Hyun M, Schroeder C.To evaluate sedation in horses following pharmacopuncture with dexmedetomidine at acupoint Governing Vessel 24 (GV-24) compared to aquapuncture at GV-24 or an equivalent dose of dexmedetomidine administered via IV or SC routes. Unassigned: 10 adult mares received 4 treatments in a blinded, randomized, crossover design: (1) dexmedetomidine administered at GV-24 (DexmedGV24; 1 μg/kg); (2) saline administered at GV-24 (SalineGV24; 1.5 mL); (3) dexmedetomidine administered SC on the lateral neck (DexmedSC; 1 μg/kg); or (4) dexmedetomidine administered IV (DexmedIV; 1 μg/kg). Sedation scores (fa...
Dos Santos TS, Dillmann JB, Giovelli M, Elias GO, Lima RK, Cescon S, Stainki DR, Dos Santos RC, Corrêa C, Monteiro SG.This study reports the successful use of maggot therapy in four horses with traumatic wounds caused by wire fences, treated in veterinary clinics and under field conditions in southern Brazil. All lesions showed tissue necrosis, purulent discharge, and foul odor, indicating infection and delayed healing. Sterile Lucilia cuprina larvae (L1 stage) were applied directly to the wounds for 48 hours. Rapid improvement was observed, with marked reduction of necrotic tissue, exudate, and odor, and stimulation of granulation tissue in three cases. Complete wound closure occurred between 10 and 60 days ...
Calle-González N, Rivero JL, Argüelles D, Requena F, Muñoz A.Capacitive resistive electrical transfer (CRET) is a non-invasive electromagnetic diathermic technique. The effect of its application 24 h prior to exercise, compared to a sham application performed with the device off, was evaluated in 8 Spanish Purebred dressage stallions. CRET was applied bilaterally on the neck, back, and croup. The horses wore an accelerometer fixed on the sternal area during a dressage test, and spatiotemporal stride parameters, total and dorsoventral (DVAA), longitudinal (LAA), and mediolateral accelerometric activities, as well as dorsoventral displacement, were recor...
Santonastaso A, Burns T, Claffey E.The objective of this video is to demonstrate a reliable technique for obtaining diagnostic venogram images of the equine foot. Venogram studies are indicated to assess vascular contrast filling of the equine digit, primarily in cases of laminitis. Unassigned: A healthy university-owned horse was used for demonstration purposes. The protocol was approved by the Virginia Polytechnic Institute and State University IACUC. Unassigned: The horse's shoes are removed, and the foot is cleaned of debris. The horse is sedated and a medial and lateral abaxial nerve block performed. The pastern is clipped...
Vargas J.To describe the application of water treadmill exercise for equine fitness and/or rehabilitation in a scientific and clinically reasonable manner. Unassigned: Horses owned by Spy Coast Farm were used for video content. Unassigned: A literature review was performed and compiled in this brief video to provide veterinarians with a comprehensive, evidence-based guide for implementing water treadmill exercise within fitness and rehabilitation programs. Unassigned: Equine water treadmill exercise is an aerobic modality that increases distal limb flexion and range of motion, with no reported adverse ...
Stöckle SD, Kannapin DA, Merle R, Lübke-Becker A, Gehlen H. The emergence and spread of multi-drug-resistant (MDR) bacteria pose a growing threat in veterinary medicine, particularly in equine hospitals. This study investigated the colonization and infection dynamics of horses undergoing emergency laparotomy with two distinct antibiotic protocols (single-shot versus 5-day protocol) during hospitalization. Nasal swabs and fecal samples were collected from 67 horses undergoing emergency laparotomy at clinic admission as well as on postoperative days 3 and 10. These were screened for multi-drug-resistant indicator pathogens. As multi-drug-resistant indi...
Cramp P.From average school reports to a surgical residency in the USA and running a 17-vet practice, this is the unplanned route Phil Cramp took to finding a career he loves.
Medina-Bautista F, Morgaz J, Quirós-Carmona S, Caravaca-Paredes ME, Navarrete-Calvo R, Medina ALS, Gómez-Villamandos R, Granados MDM.Standing sedation in horses provides immobilization and analgesia for surgery while avoiding the high risks of general anesthesia. Ketamine at subanesthetic doses may enhance sedation and reduce xylazine requirements, but evidence in clinical settings is limited. In a randomized blinded trial, we evaluated whether adding a low-dose ketamine infusion could reduce the xylazine dose required for effective sedation during standing ventriculocordectomy and laryngoplasty. Fifty-one horses were randomly assigned to sedation with xylazine alone (SX group) or xylazine plus ketamine (KX group) in a cont...
Mendoza Flores JE, Terrazas A, Lara Sagahon AV, Aleman M.Parasympathetic tone activity (PTA) and its association with behavior in conscious versus anesthesia in horses are unknown. Objective: To study PTA, heart rate (HR), minimum alveolar concentration (MAC), and mean arterial pressure (MAP) during castration under anesthesia. To study PTA, HR, Horse Grimace Scale (HGS), locomotor activity (LA), and behavior in conscious horses before and after castration. Methods: Pilot study including seven healthy adult horses. After habituation (days 1, 2, and 3), PTAm, HR, HGS, LA, and behavior were investigated during pre-surgery (days 4-, 5-, and one-hour pr...
Carvalho BVL, de Souza MCN, Avanza MFB, Teixeira RBC, Silva JRB, da Silva Cardoso T, Novais LGES, de Oliveira Monteiro FD, Viana RB, Monteiro BM....Access to the equine cecum is required for various therapeutic and experimental procedures, including decompression, fluid therapy, and transfaunation. Traditional approaches via laparotomy are highly invasive. This study aimed to describe a minimally invasive, laparoscopically assisted technique for cecal cannulation in standing horses. Unassigned: Seven horses underwent the procedure under sedation and paravertebral anesthesia. Two right flank accesses were created: a 10-mm laparoscopic port for visualization and a 2-cm minilaparotomy for cecal exteriorization. A Foley catheter was inserted ...
Dersch K, Kreuder A, Wang C, Troy J.Surgical site infections (SSI) are a common postoperative complication of equine orthopedic surgery and often increase morbidity and mortality. Objective: This study aimed to determine if covering surgical instruments during surgery decreased bacterial environmental air contamination during early opening of surgical packs (EOSP). The authors hypothesized that covering instruments with sterile surgical towels would reduce the total number of bacterial colonies to which the instruments were exposed. Methods: Blood agar plates (BAP) were allocated into two groups (non-covered vs. covered with a s...
Cantarelli C, Cribb NC, Delli-Rocili M, Brisson B, Zur Linden A, Caswell JL.Long-term assessment of nephrosplenic space (NSS) closure and comparisons with ultrasonography and rectal palpation are lacking. The objective was to evaluate NSS closure in 12 research horses 5 y following laparoscopic ablation, using a novel adhesion scoring system, and to assess whether rectal examination and ultrasonographic findings could predict adhesion adequacy. The NSS was examined rectal palpation and ultrasonography prior to surgery, and at 30 d and 5 y after surgery. Ultrasonographic measurements of the distances between the renal fascia and spleen were recorded. The NSS adhesions...
Bourassi S, Jack H, Florczyk A.A 4-year-old Arabian cross paint mare was referred for evaluation of chronic mucopurulent discharge and epiphora of the left eye. Ophthalmic and radiographic evaluations confirmed distal nasolacrimal duct atresia with absence of the nasolacrimal orifice. Initial minimally invasive treatments, including guidewire-assisted catheterization and diode laser photoablation, were unsuccessful. Definitive treatment was achieved using a Fogarty arterial embolectomy catheter under standing sedation. Controlled balloon inflation and deflation enabled progressive dilation of the obstructed duct, and a surg...
Oliveira LLV, Caldas MCAR, Marval LME, Marval CA, Carvalho JGS, Matos JJRT, Malacarne BD, Paz CFR.A 2-year-old mixed-breed mare was examined at the property due to swelling and fistula on the right mandible. Oral examination and radiographs revealed the presence of an apical abscess involving the 410 tooth. The tooth was extracted entirely and confirmed radiographically. One year later, the owner reported a reoccurrence of clinical signs. A new radiographic evaluation revealed a radiopaque area in the alveolar socket of the 410 tooth filled with spongy-looking bone. An extraoral approach to the alveolar socket was performed, and the content removed had the appearance of a dental element.
Ysebaert MP, Johnson J, Marie U, Campos A, Verchrerin A, Ducharme NG, Rossignol F, Luedke LK.To compare the biomechanical stability of a novel arytenoid implant (tie-bolt, TB) with a suture anchor (SA) and standard laryngoplasty using suture alone (control) in equine larynges. Methods: Randomized ex vivo controlled experimental study. Methods: Thirty equine cadaver larynges. Methods: Larynges were randomly assigned to TB, SA, or control groups (n = 10 each). The force of abduction was measured for each construct, then constructs underwent cyclic loading (5-50 N for 3000 cycles) to assess elongation, followed by load-to-failure testing to assess ultimate load, displacement, stiffn...
Campos Schweitzer A, Vercherin A, Rossignol F.To report successful stabilization of three cervical fracture types with articular involvement using different ventral cervical intervertebral fusion constructs. Methods: A 9-year-old French sport pony gelding, a 5-year-old Anglo-Arabian gelding and a 2-year-old French Warmblood mare. Methods: Short case series. Methods: Ventral cervical intervertebral fusion was performed in three cervical fracture cases: A vertebral body, a vertebral arch and a unilateral facet joint fracture. Selected implants for this purpose were an 11-hole 4.5/5.0 LCP, a three-dimensional (3D)-printed titanium cervical i...
Wenzel CJ, Mochal-King CA, Eddy AL, Bowser JE, Wills RW, Jumper WI, Claude A, Swiderski CE.The aim of this retrospective clinical study was to assess surgical duration and surgical and post-operative complications associated with Video-Assisted Thoracoscopic Surgery (VATS) and lung biopsy in horses with severe Equine Pasture Asthma (EPA) and paired control horses. Twelve horses (6 EPA-affected, 6 control) were sex, age and breed matched. Twenty-four thoracic surgeries were performed. Surgery of each matched pair (EPA-affected and healthy) was performed during asthma exacerbation (summer) and remission (winter). Surgical times were shorter with uncomplicated thoracoscopy (85 min) and...
Medina-Bautista F, Nocera I, Sánchez de Medina A, Di Franco C, Briganti A, Morgaz J, Granados MDM.The objective of this study was to evaluate the feasibility and efficacy of the ultrasound-guided thoracolumbar erector spinae plane (TL-ESP) block in standing horses. A total of 24 injections ( = 12) were performed at the L1 level using either 0.1 mL/kg of saline solution (SS group) or 2% lidocaine (LID group). The block feasibility was assessed based on needle visualization and injection time, while efficacy was evaluated through craniocaudal and dorsoventral (DV) spread using the pinprick technique over 270 min. Desensitization was observed at least once in 100% of horses in the LID group a...
AgrÃcola R, Ribeiro G, Borges J, Carvalho L, Roquet I, Carvalho LM, Prazeres J.Duodenal pathologies are underreported in equine medicine, probably not due to little occurrence, but due to the limitations of current diagnostic techniques. The aim of this report is to describe a novel duodenoscopic approach, that allows a complete visualization of a region inaccessible via standard techniques. Gastroduodenoscopy was performed in a clinical setting in 60 adult horses referred to us due to complaints suspected to be related to gastroduodenal diseases. The initial steps of the procedure were performed using previously published techniques. Once the video-endoscope reached the...
Coles GC, Bauer C, Borgsteede FH, Geerts S, Klei TR, Taylor MA, Waller PJ.Methods have been described to assist in the detection of anthelmintic resistance in strongylid nematodes of ruminants, horses and pigs. Two tests are recommended, an in vivo test, the faecal egg count reduction test for use in infected animals, and an in vitro test, the egg hatch test for detection of benzimidazole resistance in nematodes that hatch shortly after embryonation. Anaerobic storage for submission of faecal samples from the field for use in the in vitro test is of value and the procedure is described. The tests should enable comparable data to be obtained in surveys in all parts o...
Dalla Costa E, Minero M, Lebelt D, Stucke D, Canali E, Leach MC.The assessment of pain is critical for the welfare of horses, in particular when pain is induced by common management procedures such as castration. Existing pain assessment methods have several limitations, which reduce the applicability in everyday life. Assessment of facial expression changes, as a novel means of pain scoring, may offer numerous advantages and overcome some of these limitations. The objective of this study was to develop and validate a standardised pain scale based on facial expressions in horses (Horse Grimace Scale [HGS]). Results: Forty stallions were assigned to one of ...
De Briyne N, Atkinson J, Pokludová L, Borriello SP.The Heads of Medicines Agencies and the Federation of Veterinarians of Europe undertook a survey to gain an insight into European prescribing of antibiotics for animals, in particular to highlight the diseases for which antibiotics are most commonly said to be prescribed and which different classes, including human critically important antibiotics (CIAs). The survey was completed by 3004 practitioners from 25 European countries. Many older antibiotics (eg, penicillins, tetracyclines) are cited most frequently as the prescribed classes to treat the main food producing species. The frequency of ...
Webb JG, Chandavimol M, Thompson CR, Ricci DR, Carere RG, Munt BI, Buller CE, Pasupati S, Lichtenstein S.Percutaneous aortic valve implantation by an antegrade transvenous approach has been described but is problematic. Retrograde prosthetic aortic valve implantation via the femoral artery has potential advantages. Percutaneous prosthetic aortic valve implantation via the femoral arterial approach is described and the initial experience reported. Results: The valve prosthesis is constructed from a stainless steel stent with an attached trileaflet equine pericardial valve and a fabric cuff. After routine aortic balloon valvuloplasty, a 22F or 24F sheath is advanced from the femoral artery to the a...
Fortier LA, Potter HG, Rickey EJ, Schnabel LV, Foo LF, Chong LR, Stokol T, Cheetham J, Nixon AJ.The purpose of this study was to compare the outcomes of treatment with bone marrow aspirate concentrate, a simple, one-step, autogenous, and arthroscopically applicable method, with the outcomes of microfracture with regard to the repair of full-thickness cartilage defects in an equine model. Methods: Extensive (15-mm-diameter) full-thickness cartilage defects were created on the lateral trochlear ridge of the femur in twelve horses. Bone marrow was aspirated from the sternum and centrifuged to generate the bone marrow concentrate. The defects were treated with bone marrow concentrate and mic...
Harr KE, Flatland B, Nabity M, Freeman KP.As all laboratory equipment ages and contains components that may degrade with time, initial and periodically scheduled performance assessment is required to verify accurate and precise results over the life of the instrument. As veterinary patients may present to general practitioners and then to referral hospitals (both of which may each perform in-clinic laboratory analyses using different instruments), and given that general practitioners may send samples to reference laboratories, there is a need for comparability of results across instruments and methods. Allowable total error (TEa ) is ...
Keegan KG, Dent EV, Wilson DA, Janicek J, Kramer J, Lacarrubba A, Walsh DM, Cassells MW, Esther TM, Schiltz P, Frees KE, Wilhite CL, Clark JM....Previous studies have suggested that agreement between equine veterinarians subjectively evaluating lameness in horses is low. These studies were limited to small numbers of horses, evaluating movement on the treadmill or to evaluating previously-recorded videotape. Objective: To estimate agreement between equine practitioners performing lameness evaluations in horses in the live, over ground setting. Methods: 131 mature horses were evaluated for lameness by 2-5 clinicians (mean 3.2) with a weighted-average of 18.7 years of experience. Clinicians graded each limb using the AAEP lameness scale ...
Ferris DJ, Frisbie DD, Kisiday JD, McIlwraith CW, Hague BA, Major MD, Schneider RK, Zubrod CJ, Kawcak CE, Goodrich LR.To report outcome of horses with femorotibial lesions (meniscal, cartilage or ligamentous) treated with surgery and intra-articular administration of autologous bone marrow derived mesenchymal stem cells (BMSCs). Methods: Prospective case series. Methods: Horses (n = 33). Methods: Inclusion criteria included horses that had lameness localized to the stifle by diagnostic anesthesia, exploratory stifle arthroscopy and subsequent intra-articular administration of autologous BMSCs. Case details and follow-up were gathered from medical records, owner, trainer or veterinarian. Outcome was defined as...
Asplin KE, Sillence MN, Pollitt CC, McGowan CM.The purpose of this study was to determine the effects of prolonged administration of insulin, whilst maintaining normal glucose concentrations, on hoof lamellar integrity in vivo on healthy ponies with no known history of laminitis or insulin resistance. Nine clinically healthy, unrelated ponies were randomly allocated to either a treatment group (n =5; 5.9+/-1.7 years) or control group (n =4; 7.0+/-2.8 years). The treatment group received insulin via a euglycaemic hyperinsulinaemic clamp technique modified and prolonged for up to 72 h. Control ponies were infused with an equivalent volume of...
Frisbie DD, Morisset S, Ho CP, Rodkey WG, Steadman JR, McIlwraith CW.Microfracture of full-thickness articular defects has been shown to significantly enhance the amount of repair tissue. However, there is a suggestion that leaving calcified cartilage inhibits this repair response. Objective: Removal of the calcified cartilage with retention of subchondral bone enhances the amount of attachment of the repair tissue compared with retention of the calcified cartilage layer. Methods: Controlled laboratory study. Methods: There were 1-cm(2) articular cartilage defects made in 12 skeletally mature horses on the axial weightbearing portion of both medial femoral cond...
Goodrich LR, Hidaka C, Robbins PD, Evans CH, Nixon AJ.Gene therapy with insulin-like growth factor-1 (IGF-1) increases matrix production and enhances chondrocyte proliferation and survival in vitro. The purpose of this study was to determine whether arthroscopically-grafted chondrocytes genetically modified by an adenovirus vector encoding equine IGF-1 (AdIGF-1) would have a beneficial effect on cartilage healing in an equine femoropatellar joint model. A total of 16 horses underwent arthroscopic repair of a single 15 mm cartilage defect in each femoropatellar joint. One joint received 2 x 10(7) AdIGF-1 modified chondrocytes and the contralateral...
Kaplan RM, Denwood MJ, Nielsen MK, Thamsborg SM, Torgerson PR, Gilleard JS, Dobson RJ, Vercruysse J, Levecke B.The faecal egg count reduction test (FECRT) remains the method of choice for establishing the efficacy of anthelmintic compounds in the field, including the diagnosis of anthelmintic resistance. We present a guideline for improving the standardization and performance of the FECRT that has four sections. In the first section, we address the major issues relevant to experimental design, choice of faecal egg count (FEC) method, statistical analysis, and interpretation of the FECRT results. In the second section, we make a series of general recommendations that are applicable across all animals ad...
Kasashima Y, Takahashi T, Smith RK, Goodship AE, Kuwano A, Ueno T, Hirano S.Overstrain injuries to the superficial digital flexor tendon (SDFT) and suspensory ligament (SI) are among the most common musculoskeletal injuries which contribute to the considerable wastage of racing Thoroughbreds. Many epidemiological studies have demonstrated the prevalence of and risk factors for tendon injury when racing but have not included those injuries sustained during training. However, since tendon injury during training is seen commonly in clinical practice, it is appropriate to determine the overall prevalence of tendon injury sustained during both training and racing. Objectiv...
Metallinos DL, Bowling AT, Rine J.Lethal White Foal Syndrome is a disease associated with horse breeds that register white coat spotting patterns. Breedings between particular spotted horses, generally described as frame overo, produce some foals that, in contrast to their parents, are all white or nearly all white and die shortly after birth of severe intestinal blockage. These foals have aganglionosis characterized by a lack of submucosal and myenteric ganglia from the distal small intestine to the large intestine, similar to human Hirschsprung Disease. Some sporadic and familial cases of Hirschsprung Disease are due to muta...
van der Kolk JH, Endimiani A, Graubner C, Gerber V, Perreten V.Acinetobacter spp. are aerobic, rod-shaped, Gram-negative bacteria belonging to the Moraxellaceae family of the class Gammaproteobacteria and are considered ubiquitous organisms. Among them, Acinetobacter baumannii is the most clinically significant species with an extraordinary ability to accumulate antimicrobial resistance and to survive in the hospital environment. Recent reports indicate that A. baumannii has also evolved into a veterinary nosocomial pathogen. Although Acinetobacter spp. can be identified to species level using matrix-assisted laser desorption/ionisation time-of-flight mas...
Kurien BT, Everds NE, Scofield RH.Animal urine collection is a vital part of veterinary practice for ascertaining animal health and in scientific investigations for assessing the results of experimental manipulations. Untainted animal urine collection is very challenging, especially with small rodents, and is an almost impossible task under conditions of microgravity. The fundamental aspects of urine collection are: (1) ease of collection, (2) quality of sample, (3) prevention of contamination, (4) severity of procedures used, (5) levels of pain caused to the animal and (6) refinement of methods to reduce stress, pain or distr...
Marędziak M, Marycz K, Lewandowski D, Siudzińska A, Śmieszek A.The aim of this work study was to evaluate the cytophysiological activity of equine adipose-derived stem cells (ASCs) cultured under conditions of static magnetic field. Investigated cells were exposed to a static magnetic field (MF) with the intensity of 0.5 T. In order to investigate the effects of magnetic field on stem cell signaling, the localization and density and content of microvesicles (MVs) as well as morphology, ultrastructure, and proliferation rate of equine ASCs were evaluated. Results showed that potential of equine adipose-derived mesenchymal stem cells was accelerated when ma...
Richardson LE, Dudhia J, Clegg PD, Smith R.Stem cells have evoked considerable excitement in the animal-owning public because of the promise that stem cell technology could deliver tissue regeneration for injuries for which natural repair mechanisms do not deliver functional recovery and for which current therapeutic strategies have minimal effectiveness. This review focuses on the current use of stem cells within veterinary medicine, whose practitioners have used mesenchymal stem cells (MSCs), recovered from either bone marrow or adipose tissue, in clinical cases primarily to treat strain-induced tendon injury in the horse. The backgr...
de Grauw JC, van Loon JP.Accurate recognition and quantification of pain in horses is imperative for adequate pain management. The past decade has seen a much needed surge in formal development of systematic pain assessment tools for the objective monitoring of pain in equine patients. This narrative review describes parameters that can be used to detect pain in horses, provides an overview of the various pain scales developed (visual analogue scales, simple descriptive scales, numerical rating scales, time budget analysis, composite pain scales and grimace scales), and highlights their strengths and weaknesses for po...
Hitchens PL, Morrice-West AV, Stevenson MA, Whitton RC.Studies of racehorse injury or fatality in various countries have identified common, and in some cases conflicting, risk factors. We conducted a systematic search of the relevant literature published from 1990 to 2017. Peer-reviewed articles were included if they reported the incidence of fatal or catastrophic musculoskeletal injury (CMI) in Thoroughbred flat races (n=21) or risk factors for CMI (n=65). Pooled effect sizes were estimated using the random-effects DerSimonian-Laird model. The pooled incidence of CMI was 1.17 (95% confidence interval 0.90, 1.44) per 1000 race starts. Almost 300 f...
Weese JS, Rousseau J, Willey BM, Archambault M, McGeer A, Low DE.Methicillin-resistant Staphylococcus aureus (MRSA) is an emerging equine pathogen. To attempt to control nosocomial and zoonotic transmission, an MRSA screening program was established for all horses admitted to the Ontario Veterinary College Veterinary Teaching Hospital, whereby nasal screening swabs were collected at admission, weekly during hospitalization, and at discharge. MRSA was isolated from 120 (5.3%) of 2,283 horses: 61 (50.8%) at the time of admission, 53 (44.2%) during hospitalization, and 6 from which the origin was unclear because an admission swab had not been collected. Clinic...
Endimiani A, Hujer KM, Hujer AM, Bertschy I, Rossano A, Koch C, Gerber V, Francey T, Bonomo RA, Perreten V.We investigated whether Acinetobacter baumannii isolates of veterinary origin shared common molecular characteristics with those described in humans. Methods: Nineteen A. baumannii isolates collected in pets and horses were analysed. Clonality was studied using repetitive extragenic palindromic PCR (rep-PCR) and multilocus sequence typing (MLST). PCR and DNA sequencing for various β-lactamase, aminoglycoside-modifying enzyme, gyrA and parC, ISAba1 and IS1133, adeR and adeS of the AdeABC efflux pump, carO porin and class 1/2/3 integron genes were performed. Results: Two main clones [A (n =â€...
O'Mahony R, Abbott Y, Leonard FC, Markey BK, Quinn PJ, Pollock PJ, Fanning S, Rossney AS.Reports of methicillin-resistant Staphylococcus aureus (MRSA) in animals have become more frequent in recent years. This paper documents the recovery of MRSA from animals with respiratory, urinary tract or wound infection and from animals subjected to surgical procedures following treatment in one veterinary hospital and 16 private veterinary clinics in different geographical locations throughout Ireland. MRSA was recovered from 25 animals comprising 14 dogs, eight horses, one cat, one rabbit and a seal, and also from 10 attendant veterinary personnel. Clinical susceptibility testing suggested...
Hoynowski SM, Fry MM, Gardner BM, Leming MT, Tucker JR, Black L, Sand T, Mitchell KE.Stem cells are being evaluated in numerous human clinical trials and are commercially used in veterinary medicine to treat horses and dogs. Stem cell differentiation, homing to disease sites, growth and cytokine factor modulation, and low antigenicity contribute to their therapeutic success. Bone marrow and adipose tissue are the two most common sources of adult-derived stem cells in animals. We report on the existence of an alternative source of primitive, multipotent stem cells from the equine umbilical cord cellular matrix (Wharton's jelly). Equine umbilical cord matrix (EUCM) cells can be ...
Robin CA, Ireland JL, Wylie CE, Collins SN, Verheyen KL, Newton JR.Few data are available on the prevalence of obesity in the general equine population of Great Britain (GB), and its associated risk factors. Objective: To estimate the prevalence of owner-reported obesity in veterinary-registered horses and ponies in GB, and identify factors associated with obesity. Methods: A cross-sectional survey of horse/pony owners in GB was undertaken using a postal questionnaire. Methods: Thirty veterinary practices randomly selected horse/pony owners to complete a self-administered postal questionnaire. Owners estimated body condition score using a modified Carroll and...
Serra Bragança FM, Rhodin M, van Weeren PR.Quantitative gait analysis has the potential to offer objective and unbiased gait information that can assist clinical decision-making. In recent years, a growing number of gait analysis systems have come onto the market, highlighting the demand for such technology in equine orthopaedics. However, it is imperative that the measured variables which are used as outcome parameters are supported by scientific evidence and that the interpretation of such measurements is backed by a proper understanding of the biomechanical principles of equine locomotion. This review, which is based on studies on e...
Bakheit MA, Torra D, Palomino LA, Thekisoe OM, Mbati PA, Ongerth J, Karanis P.Three LAMP (loop-mediated isothermal DNA amplification) assays were applied to detect Cryptosporidium species DNA in a total number of 270 fecal samples originating from cattle, sheep and horses in South Africa. DNA was extracted from 0.5 g of fecal material. Results of LAMP detection were compared to those obtained by nested PCR targeting the Cryptosporidium 18 small subunit rRNA (18S) gene. All samples were negative by nested PCR, while up to one-third of samples were positive by LAMP assays. The SAM-1 LAMP assay, shown to detect C. parvum, C. hominis and C. meleagridis, amplified Cryptospor...
