Analyze Diet

Topic:Veterinary Science

Veterinary science and horses encompass the study and application of medical, surgical, and therapeutic practices to maintain and improve the health and welfare of equines. This field addresses a wide range of topics, including disease prevention, diagnosis, and treatment, as well as nutrition, reproduction, and behavior. Research in veterinary science for horses often involves understanding the pathophysiology of equine-specific diseases, developing advanced diagnostic techniques, and improving treatment protocols. This page gathers peer-reviewed research studies and scholarly articles that explore various aspects of veterinary science related to horses, providing insights into the latest advancements and methodologies in equine healthcare.
Molecular detection of tick-borne rickettsial pathogens in ticks collected from domestic animals from Cauca, Colombia.
Acta tropica    November 20, 2022   Volume 238 106773 doi: 10.1016/j.actatropica.2022.106773
Martínez Díaz HC, Gil-Mora J, Betancourt-Ruiz P, Silva-Ramos CR, Matiz-González JM, Villalba-Perez MA, Ospina-Pinto MC, Ramirez-Hernández A....Some hard ticks' species can act as vectors of a wide variety of pathogens of human and animal importance such as Anaplasma, Ehrlichia and Rickettsia spp. In Colombia, a total of forty-six tick species have been described, and some of them have been implicated as vectors of some infectious agents. The department of Cauca is one of the thirty-two departments of Colombia. Most of its population lives in rural areas and depends on agriculture as the main economic activity, favoring exposure to ticks and tick-borne pathogens. Thus, the present study aimed to determine the tick species and tick-bor...
Zoonotic RVA: State of the Art and Distribution in the Animal World.
Viruses    November 18, 2022   Volume 14, Issue 11 2554 doi: 10.3390/v14112554
Rotavirus species A (RVA) is a pathogen mainly affecting children under five years old and young animals. The infection produces acute diarrhea in its hosts and, in intensively reared livestock animals, can cause severe economic losses. In this study, we analyzed all RVA genomic constellations described in animal hosts. This review included animal RVA strains in humans. We compiled detection methods, hosts, genotypes and complete genomes. RVA was described in 86 animal species, with 52% (45/86) described by serology, microscopy or the hybridization method; however, strain sequences were not de...
Short Communication: changes in gait after 12 wk of shoeing in previously barefoot horses.
Journal of animal science    November 17, 2022   Volume 101 skac374 doi: 10.1093/jas/skac374
Panos KE, Morgan K, Gately R, Wilkinson J, Uden A, Reed SA.Farriery can impact gait symmetry and lameness outcomes, but there is limited scientific data documenting these effects. We hypothesized that shoeing previously barefoot horses with plain stamp shoes on the hind hooves would increase gait symmetry, alter hock angles and increase range of motion, and improve lameness scores more than shoeing with traditional fullered shoes. At the start of the study, gait symmetry via wireless inertial motion sensors (IMS), kinematic gait analysis (hock angle and range of motion), and American Association for Equine Practitioner's (AAEP) lameness scoring were c...
Effects of different grains on bacterial diversity and enzyme activity associated with digestion of starch in the foal stomach.
BMC veterinary research    November 17, 2022   Volume 18, Issue 1 407 doi: 10.1186/s12917-022-03510-2
Li XB, Huang XX, Li Q, Li XY, Li JH, Li C, He LJ, Jing HX, Yang KL.Compared with the stomach of ruminant cattle, the stomach of horse is small and mainly for chemical digestion, but the microorganisms in the stomach play an important role in maintaining the homeostasis of the internal environment. Due to the complexity of the microbes in the stomach, little is known about the diversity and structure of bacteria in the equine stomach. Grains are the main energy source for plant-eating livestock and energy is derived through enzymatic hydrolysis of grains into glucose or their microbial fermentation into Volatile fatty acids (VFA). However, the mechanism throug...
Can Sound Alone Act as a Virtual Barrier for Horses? A Preliminary Study.
Animals : an open access journal from MDPI    November 15, 2022   Volume 12, Issue 22 doi: 10.3390/ani12223151
Janicka W, Wilk I, Próchniak T, Janczarek I.Virtual fencing is an innovative alternative to conventional fences. Different systems have been studied, including electric-impulse-free systems. We tested the potential of self-applied acoustic stimulus in deterring the horses from further movement. Thirty warmblood horses were individually introduced to a designated corridor leading toward a food reward (variant F) or a familiar horse (variant S). As the subject reached a distance of 30, 15 or 5 m from a finish line, an acute alarming sound was played. Generally, a sudden and unknown sound was perceived by horses as a threat causing an incr...
The Innovative Informatics Approaches of High-Throughput Technologies in Livestock: Spearheading the Sustainability and Resiliency of Agrigenomics Research.
Life (Basel, Switzerland)    November 15, 2022   Volume 12, Issue 11 1893 doi: 10.3390/life12111893
Suminda GGD, Ghosh M, Son YO.For more than a decade, next-generation sequencing (NGS) has been emerging as the mainstay of agrigenomics research. High-throughput technologies have made it feasible to facilitate research at the scale and cost required for using this data in livestock research. Scale frameworks of sequencing for agricultural and livestock improvement, management, and conservation are partly attributable to innovative informatics methodologies and advancements in sequencing practices. Genome-wide sequence-based investigations are often conducted worldwide, and several databases have been created to discover ...
Journeys, Journey Conditions, and Welfare Assessment of Broken (Handled) Horses on Arrival at Italian Slaughterhouses.
Animals : an open access journal from MDPI    November 12, 2022   Volume 12, Issue 22 3122 doi: 10.3390/ani12223122
Felici M, Nanni Costa L, Zappaterra M, Bozzo G, Di Pinto P, Minero M, Padalino B.During horse transportation, the journey conditions are considered a welfare risk. This study aimed to document journeys, journey conditions, and welfare status of handled horses on arrival at two different slaughterhouses in Northern and Southern Italy, to find possible associations between journey conditions and welfare problems. The welfare status of 613 draft-breed and light-breed horses from 32 different journeys was evaluated on arrival at the slaughterhouses with a standardized protocol, using animal-based (ABMs) and environmental-based (EBMs) measures. The drivers’ skills and vehicle...
Hoof Matters: Developing an Athletic Thoroughbred Hoof.
Animals : an open access journal from MDPI    November 11, 2022   Volume 12, Issue 22 3119 doi: 10.3390/ani12223119
Hobbs SJ, Curtis S, Martin J, Sinclair J, Clayton HM.Conformation of the hooves and distal limbs of foals and factors influencing their morphological development have not been reported in detail for the Thoroughbred breed. In this paper we explore morphogenesis of the equine distal limb in Thoroughbred foals with emphasis on adaptations in response to weight bearing early in life that prepare the foal for an athletic career. Novel data from four studies are presented chronologically during key time periods to illustrate specific aspects of distal limb growth and adaptation. Dorsal epidermal thickness increased from 2.84 ± 0.41 mm in utero to 4....
Identification of mare milk adulteration with cow milk by liquid chromatography-high resolution mass spectrometry based on proteomics and metabolomics approaches.
Food chemistry    November 10, 2022   Volume 405, Issue Pt B 134901 doi: 10.1016/j.foodchem.2022.134901
Ji Z, Zhang J, Deng C, Hu Z, Du Q, Guo T, Wang J, Fan R, Han R, Yang Y.Mare milk(MM)production is lower than that of cow milk (CM), making it a premium product. Unfortunately, sellers adulterate MM with cheap CM to unscrupulously gain more profits. This study aimed to analyze MM adulteration with CM using proteomics and metabolomics. Using univariate and multivariate statistical analysis, the relative abundances of certain proteins of caseins and secretoglobin family 1D member were found to be higher with a higher CM admixture percentage. In addition, several metabolites, such as orotic and 4-aminonicotinic acids, increased in the mare-cow milk mixture with an in...
Evaluation of Strategies to Reduce Equine Strongyle Infective Larvae on Pasture and Study of Larval Migration and Overwintering in a Nordic Climate.
Animals : an open access journal from MDPI    November 10, 2022   Volume 12, Issue 22 3093 doi: 10.3390/ani12223093
Osterman-Lind E, Hedberg Alm Y, Hassler H, Wilderoth H, Thorolfson H, Tydén E.Horses, as grazing animals, are inadvertently exposed to intestinal parasites that, if not controlled, may cause disease. However, the indiscriminate use of anthelmintic drugs has led to drug resistance, highlighting the need for pasture-management practices to reduce the level of parasitic exposure and lessen reliance on drugs. The efficacy of such methods depends both on the epidemiology of the parasites and the prevailing weather conditions. The aim of the study was to investigate the effect of faecal removal and harrowing on reducing the number of parasite larvae in herbage. Moreover, the ...
Effect of Hay Steaming on the Estimated Precaecal Digestibility of Crude Protein and Selected Amino Acids in Horses.
Animals : an open access journal from MDPI    November 10, 2022   Volume 12, Issue 22 3092 doi: 10.3390/ani12223092
Pisch C, Wensch-Dorendorf M, Schwarzenbolz U, Henle T, Greef JM, Zeyner A.Steaming hay is increasingly used to treat low-quality forage because it was proven to reduce inhalable allergens such as mould spores, bacteria, and airborne dust particles. Preliminary results have shown a substantial loss of precaecal (pc) digestibility (D) of crude protein (CP) and amino acids (AA). For this purpose, six different batches of hay from central Germany were divided into four subsamples, and each one was individually steamed. Native hay and four replicates of each steamed subsample were analysed for CP, AA, neutral detergent insoluble crude protein (NDICP), neutral detergent s...
A Review of Radiomics and Artificial Intelligence and Their Application in Veterinary Diagnostic Imaging.
Veterinary sciences    November 8, 2022   Volume 9, Issue 11 620 doi: 10.3390/vetsci9110620
Bouhali O, Bensmail H, Sheharyar A, David F, Johnson JP.Great advances have been made in human health care in the application of radiomics and artificial intelligence (AI) in a variety of areas, ranging from hospital management and virtual assistants to remote patient monitoring and medical diagnostics and imaging. To improve accuracy and reproducibility, there has been a recent move to integrate radiomics and AI as tools to assist clinical decision making and to incorporate it into routine clinical workflows and diagnosis. Although lagging behind human medicine, the use of radiomics and AI in veterinary diagnostic imaging is becoming more frequent...
Exploring human behavior change in equine welfare: Insights from a COM-B analysis of the UK’s equine obesity epidemic.
Frontiers in veterinary science    November 8, 2022   Volume 9 961537 doi: 10.3389/fvets.2022.961537
Furtado T, Perkins E, Pinchbeck G, McGowan C, Watkins F, Christley R.While equine obesity is understood by equine professionals to be a serious and widespread welfare problem, thus far approaches to reducing the prevalence of obesity in the UK's leisure horses have mainly been limited to educating owners about the dangers of obesity in their horses. In human health, approaches to behavior change encourage holistic thinking around human behavior, recognizing the importance of the connection between the individuals' knowledge, attitudes, habits, and the social and physical environments. This study used qualitative data from interviews with horse owners and profes...
Timing of Vertical Head, Withers and Pelvis Movements Relative to the Footfalls in Different Equine Gaits and Breeds.
Animals : an open access journal from MDPI    November 7, 2022   Volume 12, Issue 21 doi: 10.3390/ani12213053
Rhodin M, Smit IH, Persson-Sjodin E, Pfau T, Gunnarsson V, Björnsdóttir S, Zetterberg E, Clayton HM, Hobbs SJ, Serra Bragança F, Hernlund E.Knowledge of vertical motion patterns of the axial body segments is a prerequisite for the development of algorithms used in automated detection of lameness. To date, the focus has been on the trot. This study investigates the temporal synchronization between vertical motion of the axial body segments with limb kinematic events in walk and trot across three popular types of sport horses (19 Warmbloods, 23 Iberians, 26 Icelandics) that are known to have different stride kinematics, and it presents novel data describing vertical motion of the axial body segments in tölting and pacing Icelandic ...
Serological Evidence of Exposure to Saint Louis Encephalitis and West Nile Viruses in Horses of Rio de Janeiro, Brazil.
Viruses    November 6, 2022   Volume 14, Issue 11 2459 doi: 10.3390/v14112459
Chalhoub FLL, Horta MAP, Alcantara LCJ, Morales A, Dos Santos LMB, Guerra-Campos V, Rodrigues CDS, Santos CC, Mares-Guia MAM, Pauvolid-Corrêa A....Infections with arboviruses are reported worldwide. Saint Louis encephalitis (SLEV) and West Nile (WNV) viruses are closely related flaviviruses affecting humans and animals. SLEV has been sporadically detected in humans, and corresponding antibodies have been frequently detected in horses throughout Brazil. WNV was first reported in western Brazil over a decade ago, has been associated with neurological disorders in humans and equines and its prevalence is increasing nationwide. Herein, we investigated by molecular and serological methods the presence of SLEV and WNV in equines from Rio de Ja...
Characterisation and prevalence of community-associated MRSA among horses, dogs, cats and their human handlers: a cross-sectional study.
Transactions of the Royal Society of Tropical Medicine and Hygiene    November 5, 2022   Volume 117, Issue 3 212-218 doi: 10.1093/trstmh/trac103
Abdulkadir A, Kabir J, Mohammed B, Olayinka B.Methicillin-resistant Staphylococcus aureus (MRSA) as an infectious organism of public health significance has evolved to a genetically distinct community-acquired MRSA with extended resistance to other than β-lactams. A cross-sectional study was conducted among 149 participants handling 446 animals (240 horses and 206 companion animals). The isolates were characterised as S. aureus and MRSA based on polymerase chain reaction detection of the nuc, mecA and mecC genes and the pvl gene for differentiation as community associated/livestock associated or hospital associated. The isolation rate of...
Serosurvey of spotted fever group Rickettsia in equids from western Pará, Amazon, Brazil.
Experimental & applied acarology    November 4, 2022   Volume 88, Issue 3-4 361-370 doi: 10.1007/s10493-022-00753-4
Minervino AHH, Andersson E, Norlander E, Moreira TR, Gennari SM, Mercado Caruso N, Moraes Filho J, Marcili A, Martins TF, Labruna MB.From a previous large epidemiological survey, we randomly selected 474 serum samples (463 horses and 11 mules) distributed among four municipalities of Pará state, Amazon region, Brazil, and from three types: farm animal, urban carthorse, and sport horse. Samples were tested by indirect fluorescence antibody test (IFAT ≥ 64) for antibodies reactive to spotted fever group (SFG) rickettsiae using Rickettsia rickettsii as crude antigens. From the 474 equids tested, 149 (31.4%) had ticks attached during sampling, belonging mostly to the species Dermacentor nitens. The overall seroprevalence...
Immune Activated Cellular Therapy for Drug Resistant Infections: Rationale, Mechanisms, and Implications for Veterinary Medicine.
Veterinary sciences    November 4, 2022   Volume 9, Issue 11 610 doi: 10.3390/vetsci9110610
Pezzanite LM, Chow L, Strumpf A, Johnson V, Dow SW.Antimicrobial resistance and biofilm formation both present challenges to treatment of bacterial infections with conventional antibiotic therapy and serve as the impetus for development of improved therapeutic approaches. Mesenchymal stromal cell (MSC) therapy exerts an antimicrobial effect as demonstrated in multiple acute bacterial infection models. This effect can be enhanced by pre-conditioning the MSC with Toll or Nod-like receptor stimulation, termed activated cellular therapy (ACT). The purpose of this review is to summarize the current literature on mechanisms of antimicrobial activity...
Surveillance and Molecular Characterization of SARS-CoV-2 Infection in Non-Human Hosts in Gujarat, India.
International journal of environmental research and public health    November 3, 2022   Volume 19, Issue 21 14391 doi: 10.3390/ijerph192114391
Kumar D, Antiya SP, Patel SS, Pandit R, Joshi M, Mishra AK, Joshi CG, Patel AC.Since December 2019, Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) has been spreading worldwide, triggering one of the most challenging pandemics in the human population. In light of the reporting of this virus in domestic and wild animals from several parts of the world, a systematic surveillance study was conceptualized to detect SARS-CoV-2 among species of veterinary importance. Nasal and/or rectal samples of 413 animals (dogs n= 195, cattle n = 64, horses n = 42, goats n = 41, buffaloes n = 39, sheep n = 19, cats n = 6, camels n = 6, and a monkey n = 1) were collected from d...
Selection of species specific panel of reference genes in peripheral blood mononuclear cells of native livestock species adapted to trans-Himalayan region of Leh-Ladakh.
Scientific reports    November 2, 2022   Volume 12, Issue 1 18473 doi: 10.1038/s41598-022-22588-0
Tiwari M, Sodhi M, Verma P, Vivek P, Kataria RS, Niranjan SK, Bharti VK, Masharing N, Gujar G, Chanda D, Mukesh M.The identification of appropriate references genes is an integral component of any gene expression-based study for getting accuracy and reliability in data interpretation. In this study, we evaluated the expression stability of 10 candidate reference genes (GAPDH, RPL4, EEF1A1, RPS9, HPRT1, UXT, RPS23, B2M, RPS15, ACTB) in peripheral blood mononuclear cells of livestock species that are adapted to high altitude hypoxia conditions of Leh-Ladakh. A total of 37 PBMCs samples from six native livestock species of Leh-Ladakh region such as Ladakhi cattle, Ladakhi yak, Ladakhi donkey, Chanthangi goat...
Use of antimicrobials licensed for systemic administration in UK equine practice.
Equine veterinary journal    November 2, 2022   Volume 55, Issue 5 808-819 doi: 10.1111/evj.13878
Allen SE, Verheyen KLP, O'Neill DG, Brodbelt DC.Judicious antimicrobial use (AMU) is important for preserving therapeutic effectiveness. Large-scale studies of antimicrobial prescribing can provide clinical benchmarks and help identify opportunities for improved stewardship. Objective: To describe systemic AMU in UK equine practice and identify factors associated with systemic and Category B (third and fourth generation cephalosporins, quinolones and polymixins) AMU. Methods: Retrospective cohort. Methods: Anonymised electronic patient records (EPRs) for all equids attended by 39 UK veterinary practices between 1 January and 31 December 201...
Changes in Acute Phase Response Biomarkers in Racing Endurance Horses.
Animals : an open access journal from MDPI    October 31, 2022   Volume 12, Issue 21 doi: 10.3390/ani12212993
Mihelić K, Vrbanac Z, Bojanić K, Kostanjšak T, Ljubić BB, Gotić J, Vnuk D, Bottegaro NB.This study aimed to evaluate if exercise-induced acute phase response (APR) occurs in endurance horses in response to the race. The study included 23 horses competing in an endurance competition with a successfully passed clinical examination before the race. Blood samples were collected before the start and within 30 min after the end of the race. Haematological and biochemical tests were performed and correlated to acute phase biomarkers changes. Values of calprotectin and haptoglobin (Hp) decreased after the races compared to values before, while concentrations of ceruloplasmin and albumin ...
Changes in Management, Welfare, Emotional State, and Human-Related Docility in Stallions.
Animals : an open access journal from MDPI    October 30, 2022   Volume 12, Issue 21 2981 doi: 10.3390/ani12212981
Popescu S, Lazar EA, Borda C, Blaga Petrean A, Mitrănescu E.Despite an increase in awareness of their essential needs, many stallions continue to be kept in conditions limiting their social interactions and movement. To supplement the studies which highlight the effects of these practices on selected aspects of equine mental and physical wellbeing, we aimed to monitor a group of 32 adult intact stallions during their transition from tethered housing with limited outdoor access to free group housing through the lens of their overall welfare, perceived emotional status, and docility toward humans. Over three visits (before the management change, two week...
Safety and efficacy of a feed additive consisting of glyceryl polyethyleneglycol ricinoleate (PEG castor oil) for all animal species (FEFANA asbl).
EFSA journal. European Food Safety Authority    October 28, 2022   Volume 20, Issue 10 e07433 doi: 10.2903/j.efsa.2022.7433
Bampidis V, Azimonti G, Bastos ML, Christensen H, Dusemund B, Fašmon Durjava M, Kouba M, López-Alonso M, López Puente S, Marcon F, Mayo B....Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety and efficacy of glyceryl polyethyleneglycol ricinoleate (PEG castor oil) as technological feed additive for all animal species. PEG castor oil is safe at a maximum concentration in complete feed of 90 mg/kg for chickens for fattening and other minor growing poultry; 134 mg/kg for laying hens and other laying/breeding birds kept for egg production/reproduction; 121 mg/kg for turkeys for fattening; 162 mg/kg for piglets and other minor growing ; 194 mg/kg for pigs for fattening; 23...
Genetic Parameters of Performance and Conformation Traits of 3-Year-Old Warmblood Sport Horses in the Czech Republic.
Animals : an open access journal from MDPI    October 27, 2022   Volume 12, Issue 21 2957 doi: 10.3390/ani12212957
Novotna A, Birovas A, Vostra-Vydrova H, Vesela Z, Vostry L.The aim of this study was to estimate the genetic parameters of a one-day performance test together with the linear type traits of 3-year-old warmblood horses. The study of genetic parameters was based on 5958 tested horses in the period 1998-2021. A total of 22 traits of linear description, three quantitatively measured traits, and one summary mark from the performance test were tested. The model equation included the fixed effect of gender and combination effects of classifier-year of evaluation-place. A single-trait animal model was used for the estimation of heritability and genetic varian...
A Qualitative Exploration of UK Leisure Horse Owners’ Perceptions of Equine Wellbeing.
Animals : an open access journal from MDPI    October 26, 2022   Volume 12, Issue 21 doi: 10.3390/ani12212937
Smith R, Furtado T, Brigden C, Pinchbeck G, Perkins E.Human assessment of equine wellbeing is fundamental to ensuring the optimal care of domestic horses. However, terminology associated with wellbeing is still not fully defined and there are currently no validated quality of life (QoL) assessment tools. Furthermore, little is known about what equine wellbeing or QoL means to horse owners, or how their beliefs impact on the management decisions they make for their horse. This study sought to establish how UK leisure horse owners use wellbeing-related terminology by exploring their accounts within a focus group setting. Four online focus group dis...
Not just for males: Flehmen as a tool for detection of reproductive status and individual recognition across sexes in four African equid species.
Behavioural processes    October 26, 2022   Volume 203 104773 doi: 10.1016/j.beproc.2022.104773
Pluháček J, Tučková V, King SRB.Flehmen is frequently explained as part of male sexual behaviour, but it can also be associated with overmarking behaviour and thus individual recognition. We tested three explanatory hypotheses of flehmen behaviour: to detect sexual status of a female, to decide whether to overmark an individual, and to improve individual recognition. Additionally, we examined interspecific flehmen differences in the African equids. We observed 130 individuals of all 4 species among 15 groups in 5 zoos. We recorded 4445 eliminations: 142 were accompanied by flehmen and 1648 were inspected by another animal an...
Moving toward Fear-Free Husbandry and Veterinary Care for Horses.
Animals : an open access journal from MDPI    October 24, 2022   Volume 12, Issue 21 2907 doi: 10.3390/ani12212907
Carroll SL, Sykes BW, Mills PC.Husbandry and veterinary procedures have the potential to generate fear and stress in animals. In horses, the associated responses can pose a significant safety risk to the human personnel involved in the procedure, as well as to the animal itself. Traditionally, physical restraint, punishment, and/or threat of an aversive, have been the most common strategies used to achieve compliance from the horse. However, from a welfare perspective, this is less than ideal. This approach also has the potential for creating a more dangerous response from the horse in future similar situations. When caring...
Perceptions of Fear and Anxiety in Horses as Reported in Interviews with Equine Behaviourists.
Animals : an open access journal from MDPI    October 23, 2022   Volume 12, Issue 21 2904 doi: 10.3390/ani12212904
Rogers S, Bell C.One of the key welfare concerns for horses in the United Kingdom is lack of recognition of fear in horses. This study aimed to gain an understanding of how well horse care givers recognise fear and/or anxiety in horses by interviewing equine behaviourists (who interact with large numbers of horse care givers and talk to them about this topic routinely). The experiences of Animal Behaviour and Training Council (ABTC)-registered equine behaviourists working with horse caregivers were examined, including the ability of clients to recognise fear and/or anxiety in horses, how clients respond when d...
Variability of Jump Biomechanics Between Horses of Different Age and Experience Using Commercial Inertial Measurement Unit Technology.
Journal of equine veterinary science    October 22, 2022   Volume 119 104146 doi: 10.1016/j.jevs.2022.104146
Becker K, Lewczuk D.The application of commercial inertial measurement units has become popular in equestrian sports, which may help to eliminate a gap of knowledge concerning many aspects of biomechanics in training. This study employed the Seaver IMU system to measure jumping characteristics of horses with differing age-competition experience during regular training. It was hypothesized that experience level results in lower variability of jumping parameters. Twelve Warmblood horses aged 5 to 6 years with/without competition experience and 7 to 11 years with experience were investigated during regular training ...
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