Topic:Animal Studies
Animal studies involving horses encompass a range of research focused on understanding equine biology, behavior, and health. These studies often investigate various aspects of horse physiology, genetics, nutrition, and disease pathology. Researchers utilize animal studies to explore the effects of different treatments, management practices, and environmental factors on horse welfare and performance. The findings from such studies contribute to the development of improved care strategies and health interventions. This page gathers peer-reviewed research studies and scholarly articles that explore the methodologies, findings, and implications of animal studies conducted on horses, providing insights into their application in advancing equine science.
The equine mesenchymal stromal cell secretome inhibits equid herpesvirus type 1 strain Ab4 in epithelial cells. Equid herpesvirus 1 (EHV-1) outbreaks occur when virus spreads from infected horses to in-contact horses, primarily via nasal shedding. This study evaluated the efficacy of factors secreted by equine peripheral blood derived mesenchymal stromal cells (PB-MSCs), collectively named the secretome, to inhibit the growth of EHV-1 in (i) 2D epithelial cell cultures (RK-13) in vitro, (ii) 3D equine nasal explants in vitro and (iii) an EHV-1 infection mouse model in vivo. The PB-MSC secretome was found to inhibit EHV-1 in RK-13 cells as well as in the epithelium of equine nasal explants. Although the ...
Isolation of Rhodococcus equi from the gastrointestinal contents of earthworms (family Megascolecidae). Rhodococcus equi was isolated from the gastrointestinal contents of earthworms (family Megascolecidae) and their surrounding soil collected from pastures of two horse-breeding farms in Aomori Prefecture, outdoor pig pens, forest in Towada campus, orange groves and forest where wild boars (Sus scrofa) are established in Tanabe, Wakayama Prefecture. The number of R. equi in the lower gastrointestinal contents of 23 earthworms collected from our campus was significantly larger than that of the upper gastrointestinal content. The mean numbers of R. equi from the gastrointestinal contents of earthw...
[Intensity of Horses’ Reaction to Predatory Sounds]. VOR DER DOMESTIKATION HING DAS ÜBERLEBEN DER EQUIDEN VON DER FäHIGKEIT AB, SICH IHRER UMGEBUNG UND MIT IHR VERBUNDENEN GEFAHREN ANZUPASSEN. DIES BETRAF V. A. RAUBTIERE, AUF DIE DIE PFERDE SCHNELL REAGIEREN MUSSTEN. MODERNE PFERDE UNTERSCHEIDEN SICH VON IHREN VORFAHREN U. A. IN IHRER REAKTION AUF STRESSOREN. SIE WURDEN üBER JAHRHUNDERTE HINWEG SO GEZüCHTET, DASS EIN FüR MENSCHEN SICHERER UMGANG MIT IHNEN MöGLICH IST. DIES REDUZIERTE AUCH IHRE REAKTIONEN AUF ANGSTERZEUGENDE FAKTOREN. DIE STUDIE UNTERSUCHTE, OB PFERDE NOCH IMMER DIE FäHIGKEIT BESITZEN, RECHTZEITIG AUF GEFAHREN DURCH RAUBTI...
Rejection behaviour of horses for hay contaminated with meadow saffron (Colchicum autumnale L.). Extensively used grasslands are frequently utilised for hay production for equines. Especially, extensive meadows have a great variety of plant species, which may include plants that are poisonous for equines such as meadow saffron (Colchicum autumnale L.). To authors' knowledge investigations about horses` avoidance behaviour towards dried meadow saffron in hay are missing. Reports of farmers are contrary to clinical symptoms described in case reports and associated with meadow saffron in hay. Objective: The aim of this study was to determine the rejection behaviour of horses for hay contamin...
Neosaxitoxin, a Paralytic Shellfish Poison phycotoxin, blocks pain and inflammation in equine osteoarthritis. The Osteoarthritis is a chronic disease characterized by a progressive deterioration of the articular cartilage producing a strong inflammatory activity and chronic pain in patients. Horses also show osteoarthritis. Since the activation and progression of the disease are similar to that of human we developed a study model in horses. In this study, we test the effect of Neosaxitoxin, a phycotoxin from Paralytic Shellfish Poison, in the remediation of osteoarthritis equine clinical symptoms such as pain (showed in lameness) and inflammation quantifying the amounts of pro-inflammatory markers lik...
Performance and automation of ancient DNA capture with RNA hyRAD probes. DNA hybridization-capture techniques allow researchers to focus their sequencing efforts on preselected genomic regions. This feature is especially useful when analysing ancient DNA (aDNA) extracts, which are often dominated by exogenous environmental sources. Here, we assessed, for the first time, the performance of hyRAD as an inexpensive and design-free alternative to commercial capture protocols to obtain authentic aDNA data from osseous remains. HyRAD relies on double enzymatic restriction of fresh DNA extracts to produce RNA probes that cover only a fraction of the genome and can serve a...
Genome-wide scans for signatures of selection in Mangalarga Marchador horses using high-throughput SNP genotyping. The detection of signatures of selection in genomic regions provides insights into the evolutionary process, enabling discoveries regarding complex phenotypic traits. In this research, we focused on identifying genomic regions affected by different selection pressures, mainly highlighting the recent positive selection, as well as understanding the candidate genes and functional pathways associated with the signatures of selection in the Mangalarga Marchador genome. Besides, we seek to direct the discussion about genes and traits of importance in this breed, especially traits related to the typ...
Factors Associated with Fatality in Ontario Thoroughbred Racehorses: 2003-2015. Ontario's Alcohol and Gaming Commission records equine racing fatalities through its Equine Health Program. The present study examined all Thoroughbred fatalities from 2003 to 2015, inclusive, to identify associations. Official records and details of fatalities were combined in multivariable logistic regression modelling of 236,386 race work-events (433 fatalities), and 459,013 workout work-events (252 fatalities). Fatality rates were 2.94/1000 race starts (all fatalities) and 1.96/1000 (breakdowns only) with an overall rate of 2.61% or 26.1 fatalities/1000 horses. Comparison with published re...
Genomic Regions Associated with the Position and Number of Hair Whorls in Horses. The position and number of hair whorls have been associated with the behavior, temperament, and laterality of horses. The easy observation of whorls assists in the prediction of reactivity, and thus permits the development of better measures of handling, training, mounting, and riding horses. However, little is known about the genetics involved in the formation of hair whorls. Therefore, the aim of this study was to perform a genome-wide association analysis to identify chromosome regions and candidate genes associated with hair whorl traits. Data from 342 Quarter Horses genotyped for approxim...
The need for formal reflexivity in conservation science. Conservation issues are often complicated by sociopolitical controversies that reflect competing philosophies and values regarding natural systems, animals, and people. Effective conservation outcomes require managers to engage myriad influences (social, cultural, political, and economic, as well as ecological). The contribution of conservation scientists who generate the information on which solutions rely is constrained if they are unable to acknowledge how personal values and disciplinary paradigms influence their research and conclusions. Conservation challenges involving controversial spe...
Zoonotic giardiasis: an update. Giardia duodenalis is a common intestinal parasite in various hosts, with the disease giardiasis being a zoonosis. The use of molecular typing tools has improved our understanding of the distribution and zoonotic potential of G. duodenalis genotypes in different animals. The present review summarizes recent data on the distribution of G. duodenalis genotypes in humans and animals in different areas. The dominance of G. duodenalis assemblages A and B in humans and common occurrence of host-adapted assemblages in most domesticated animals suggests that zoonotic giardiasis is probably less common...
Investigation of Factors Influencing Thoroughbred Horses’ Racing Career Length in Turkey. The aim of this research was to determine the average length of a Thoroughbred horse's racing career in Turkey using survival functions of Thoroughbred horses with various characteristics. In addition, the aim was to identify risk factors that could influence the duration of a Thoroughbred horse's racing career and develop a survival model that took these factors into account. A total of 11,721 Thoroughbred horses born in 2007 and later were included in the study population. The horses involved in the study were followed for a minimum of 1 year. Kaplan-Meier analysis was used to calculate the ...
Horse Jumping and Dressage Training Activity Detection Using Accelerometer Data. Equine training activity detection will help to track and enhance the performance and fitness level of riders and their horses. Currently, the equestrian world is eager for a simple solution that goes beyond detecting basic gaits, yet current technologies fall short on the level of user friendliness and detection of main horse training activities. To this end, we collected leg accelerometer data of 14 well-trained horses during jumping and dressage trainings. For the first time, 6 jumping training and 25 advanced horse dressage activities are classified using specifically developed models base...
Does the type of exercise affect tryptophan catabolism in horses? Tryptophan (Trp) is an essential amino acid which metabolises via the kynurenine pathway to generate a number of bioactive substances referred to as kynurenines. Among those are 3-hydroxykynurenine (3-HKyn) and quinolinic acid, which are neurotoxic, as well as kynurenic acid (Kyna) and xanthurenic acid (XA), which, similarly to nicotinamide (NAm), show neuroprotective and anti-depressive effects. Routine exercise is known to modulate Trp metabolism in skeletal muscle and is thus believed to reduce the risk of depressive states in humans and laboratory animals. Analogously, it was hypothesised ...
Gastrin and Nitric Oxide Production in Cultured Gastric Antral Mucosa Are Altered in Response to a Gastric Digest of a Dietary Supplement. organ culture can provide insight into isolated mucosal responses to particular environmental stimuli. The objective of the present study was to investigate the impact of a prolonged culturing time as well as the addition of acidic gastric fluid into the environment of cultured gastric antral tissue to evaluate how altering the commonly used neutral environment impacted tissue. Furthermore, we aimed to investigate the impact of G's Formula, a dietary supplement for horses, on the secretion of gastrin, interleukin1-beta (IL-1β), and nitric oxide (NO). These biomarkers are of interest due to ...
16 Years of breed management brings substantial improvement in population genetics of the endangered Cleveland Bay Horse. The consequences of poor breed management and inbreeding can range from gradual declines in individual productivity to more serious fertility and mortality concerns. However, many small and closed groups, as well as larger unmanaged populations, are plagued by genetic regression, often due to a dearth in breeding support tools which are accessible and easy to use in supporting decision-making. To address this, we have developed a population management tool (BCAS, Breed Conservation and Management System) based on individual relatedness assessed using pedigree-based kinship, which offers breedi...
The primary visual cortex of Cetartiodactyls: organization, cytoarchitectonics and comparison with perissodactyls and primates. Cetartiodactyls include terrestrial and marine species, all generally endowed with a comparatively lateral position of their eyes and a relatively limited binocular field of vision. To this day, our understanding of the visual system in mammals beyond the few studied animal models remains limited. In the present study, we examined the primary visual cortex of Cetartiodactyls that live on land (sheep, Père David deer, giraffe); in the sea (bottlenose dolphin, Risso's dolphin, long-finned pilot whale, Cuvier's beaked whale, sperm whale and fin whale); or in an amphibious environment (hippopotam...
[Variability of the D-Loop Sequence of Mitochondrial DNA in Transbaikal Horses]. The Zabaikalskaya horse is an indigenous breed of horses from Siberia with diverse use. It is characterized by endurance and good adaptability to year-round herd maintenance in the harsh conditions of the Baikal steppes. To determine the genetic characteristics of the maternal lineage of the Zabaikalskaya horse breed based on mitochondrial DNA polymorphisms, we collected hair samples from 31 horses belonging to breeding farms in the Trans-Baikal Territory. Analysis of the 530 bp sequence of the mtDNA D-loop was performed using the maximum composite likelihood (MCL) model in combination with bo...
Dose-Dependent Increase in Whole Blood Omega-3 Fatty Acid Concentration in Horses Receiving a Marine-Based Fatty-Acid Supplement. The objective of this study was to determine the effects of an oral, commercially available, marine based omega-3 fatty acid (n3-FA) supplement on fatty acid characteristics in horse whole blood. Fifty healthy, adult horses of various light breeds were assigned to one of two treatment groups: Group 1 receiving 7.5g/day of the test supplement, and Group 2 receiving 15g/day of the test supplement. The supplement contained 0.092g docosahexaenoic acid (DHA) and 0.148g eicosapentaenoic acid (EPA) per gram. Therefore, Group 1 received 1.11g of EPA and 0.69g of DHA daily, while Group 2 received 2.22g...
Studies of Peculiar Mg-Containing and Oscillating Bioapatites in Sheep and Horse Teeth. New types of biological apatites have been discovered in molar sheep and horse teeth and are divided in two types. In the first and more general type, the release of Mg ions is parallel to the changes in composition of apatite leading to a final stoichiometric ratio of Ca to P ions, going from dentin depth towards the boundary of enamel with air. Inside dentin, another apatite sub-types were discovered with alternating layers of Mg-rich and C-rich apatites. The approximate formal stoichiometric relationships for these peculiar types of bioapatites are suggested. We identified two kinds of ion-...
Comparison of American Quarter Horses Competing in Western Pleasure, Hunter under Saddle, and Reining Using Linear Traits. To investigate differences in American Quarter Horses conformation suggesting specialization and subpopulations within the breed, a total of 45 horses were studied. These horses were classified according to their specific sport discipline: Western Pleasure (WPL, n = 15), Hunter under Saddle (HUS, n = 15), and Reining (RN, n = 15). Fifteen linear traits (comprising lengths, angles, and circumferences) were measured analogically and photometrically. Afterwards, 9 ratios and differences were calculated, so that a total of 24 traits were examined. The results showed significant differences between...
Morphological differentiation of bovine and equine hair for species’ identification in forensic veterinary investigations. In this paper a morphological differentiation tool of bovine and equine hair for their identification in forensic investigations is provided. 30 animals were used, fifteen adult bovines of the "Siboney de Cuba" breed and fifteen adult equines of the "Criolla" breed. These species are the most bred in Cuba and, at the same time, the most exposed to poaching and theft crimes. Hair from the cows was sampled from the dorsal region, the lateral region and the ventral region; on the other hand, the horses' hair was sampled from the dorsal region of the body, of the mane and of the tail. The sampled ...
Probiotic potential of Lactobacillus isolated from horses and its therapeutic effect on DSS-induced colitis in mice. Inflammatory bowel disease (IBD) is a refractory disease that endangers both humans and animals. In recent times, Lactobacillus have been used to treat animal diseases. It may be a good choice to try to isolate Lactobacillus with probiotic potential to treat IBD. Equine, as a kind of hindgut fermentation animal has rich intestinal microflora, but data regarding this is scarce. The isolation of Lactobacillus with probiotic potential from equine may become a new method for the treatment of IBD. Four isolates of Lactobacillus were isolated from fresh feces of healthy male adult horses and analyze...
Exposing cryptic epitopes on the Venezuelan equine encephalitis virus E1 glycoprotein prior to treatment with alphavirus cross-reactive monoclonal antibody allows blockage of replication early in infection. Eastern equine encephalitis virus (EEEV), western equine encephalitis virus (WEEV) and Venezuelan equine encephalitis virus (VEEV) can cause fatal encephalitis in humans and equids. Some MAbs to the E1 glycoprotein are known to be cross-reactive, weakly neutralizing in vitro but can protect from disease in animal models. We investigated the mechanism of neutralization of VEEV infection by the broadly cross-reactive E1-specific MAb 1A4B-6. 1A4B-6 protected 3-week-old Swiss Webster mice prophylactically from lethal VEEV challenge. Likewise, 1A4B-6 inhibited virus growth in vitro at a pre-attachm...
Area and Resource Utilization of Group-Housed Horses in an Active Stable. The aim of this study was to analyze the utilization of different stable areas of a total of 52 group-housed horses as well as their preferred stable parts and the use of resources. The study was situated in a "HIT Active Stable" in Northern Germany for a period of 227 observation days. After dividing the whole farm area in a grid of 3 × 3 m, the dataset was examined with and without the pasture area. Furthermore, linear mixed models were applied. On average, horses used 53.2 ± 19 different squares per hour. The observation day ( < 0.001) and the covariate age ( < 0.001) had significan...
Cranial shape diversification in horses: variation and covariation patterns under the impact of artificial selection. The potential of artificial selection to dramatically impact phenotypic diversity is well known. Large-scale morphological changes in domestic species, emerging over short timescales, offer an accelerated perspective on evolutionary processes. The domestic horse (Equus caballus) provides a striking example of rapid evolution, with major changes in morphology and size likely stemming from artificial selection. However, the microevolutionary mechanisms allowing to generate this variation in a short time interval remain little known. Here, we use 3D geometric morphometrics to quantify skull morph...
The Effect of Ground Poles and Elastic Resistance Bands on Longissimus Dorsi and Rectus Abdominus Muscle Activity During Equine Walk and Trot. Core strengthening and postural stability are desired outcomes of certain therapeutic exercises performed in horses. This study aimed to quantify changes in muscle activation at a walk and trot in horses traveling over eight consecutive ground poles evenly spaced (at 30 inches for walk and 48 inches for trot) in parallel fashion in a straight line, and with hindquarter and abdominal elastic resistance bands applied at 25% stretch. Surface electromyography (sEMG) data were collected for the longissimus dorsi and rectus abdominus muscles in six horses. A 2 × 2 repeated measures ANOVA was perf...
Effect of Dose and Fasting on Oral Sugar Test Responses in Insulin Dysregulated Horses. The oral sugar test (OST) is frequently used to identify insulin dysregulated (ID) equines. The effect of fasting and varying sugar dose for the OST has been investigated in the pony but little work has been done in the horse. This study aimed to investigate (1) an OST response with access to forage continued until the time of the OST or prevented for 3 hours prior to the OST and (2) responses of ID and non-insulin dysregulated (NID) horses to two different OST doses. Twenty-one mixed-breed horses (14.8 ± 3.2 years; 574.3 ± 83.3 kg) were used in two randomized crossover studies. Seven ID and...
Equine diet during protohistoric times in the Northeast of the Iberian Peninsula: Stable isotope data (C, N) from bone collagen. The analysis of stable isotopes in bone collagen allows us to infer the diet of the animals studied. This dataset consists of isotopic signatures (δC and δN) obtained by isotope ratio mass spectrometry from the skeletal remains of 42 equines (horse, ass and their hybrids) from the Can Roqueta site (Sabadell, Northeast Iberian Peninsula). Their chronology spans from Late Bronze Age to Late Roman Period, with particular emphasis on the Early Iron Age. These animals were found in storage silos and graves and were probably sacrificed as ritual offerings. The isotopic values are accompanied by da...