Analyze Diet

Topic:Diet

Diet in horses refers to the nutritional regimen that supports the health, performance, and overall well-being of equines. It encompasses the types and amounts of feed provided, including forage, grains, supplements, and water. The dietary requirements of horses can vary based on factors such as age, breed, activity level, and health status. Proper nutrition is essential for maintaining optimal body condition, supporting growth and development, and ensuring efficient energy metabolism. This page compiles peer-reviewed research studies and scholarly articles that explore the components of equine diets, the impact of nutrition on health and performance, and the management of feeding practices in various equine disciplines.
Effects of diet-induced weight gain on insulin sensitivity and plasma hormone and lipid concentrations in horses.
American journal of veterinary research    October 3, 2009   Volume 70, Issue 10 1250-1258 doi: 10.2460/ajvr.70.10.1250
Carter RA, McCutcheon LJ, George LA, Smith TL, Frank N, Geor RJ.To determine the effects of diet-induced weight gain on glucose and insulin dynamics and plasma hormone and lipid concentrations in horses. Methods: 13 adult geldings. Methods: Horses were fed 200% of their digestible energy requirements for maintenance for 16 weeks to induce weight gain. Frequently sampled IV glucose tolerance tests were performed before and after weight gain to evaluate glucose and insulin dynamics. Adiposity (assessed via condition scoring, morphometric measurements, and subcutaneous fat depth) and plasma concentrations of insulin, glucose, nonesterified fatty acids, trigly...
Effect of live yeast culture supplementation on hindgut microbial communities and their polysaccharidase and glycoside hydrolase activities in horses fed a high-fiber or high-starch diet.
Journal of animal science    May 22, 2009   Volume 87, Issue 9 2844-2852 doi: 10.2527/jas.2008-1602
Jouany JP, Medina B, Bertin G, Julliand V.Four cecum and right ventral colon-fistulated horses were assigned in a 4 x 4 Latin square design and fed a high-fiber (HF) or a high-starch (HS) diet with or without 10 g of Saccharomyces cerevisiae (SC; CBS 493.94) containing 4.5 x 10(9) cfu/g. The HF and HS diets consisted of pelleted feeds and long wheat straw (18.0 and 3.5 g of DM.kg(-1) of BW.d(-1), respectively) given in 2 equal meals to provide an NDF:starch ratio of 3.5 and 1.0, respectively. After a 21-d adaptation period intestinal contents were collected 4 h after the morning meal on d 23 and 25 to determine bacterial and SC concen...
Optimal diet of horses with chronic exertional myopathies.
The Veterinary clinics of North America. Equine practice    March 24, 2009   Volume 25, Issue 1 121-vii doi: 10.1016/j.cveq.2008.12.001
McKenzie EC, Firshman AM.Chronic exertional rhabdomyolysis represents a syndrome of recurrent exercise-associated muscle damage in horses that arises from a variety of etiologies. Major advances have been made in the understanding of the pathophysiology of this disease, and causative genetic defects have been recently identified for two conditions-polysaccharide storage myopathy of quarter horses, paints, warm bloods, and draft breeds. Dietary management in combination with a regular exercise regimen comprises the most effective means for control of clinical signs.
Dietary management of obesity and insulin resistance: countering risk for laminitis.
The Veterinary clinics of North America. Equine practice    March 24, 2009   Volume 25, Issue 1 51-vi doi: 10.1016/j.cveq.2009.02.001
Geor RJ, Harris P.Insulin resistance (IR) and hyperinsulinemia increase risk for development of laminitis in horses and ponies. Obesity also has been associated with heightened risk for laminitis, likely by means of development of IR. Dietary factors, particularly the nonstructural carbohydrate (NSC) load, modulate risk for laminitis in these animals by means of exacerbation of IR or gastrointestinal disturbances that trigger the condition. Specific dietary management strategies to lessen risk for laminitis include caloric restriction to promote weight loss and improve insulin sensitivity in obese animals and s...
The role of nutrition in colic.
The Veterinary clinics of North America. Equine practice    March 24, 2009   Volume 25, Issue 1 67-vi doi: 10.1016/j.cveq.2008.11.003
Durham AE.Nutritional intolerances manifesting as colic in the horse may be largely explained by divergence from the diet and ingestive behaviors to which the feral ancestors of modern domesticated equids had become accustomed and adapted. High-starch diets and abrupt dietary changes are probably foremost in the risk factors for diet-associated colic in the horse and have their basis in disruption of the stability of microbial populations resident within the equine hindgut. Although some general associations between colic and diet may be inferred from several epidemiologic studies, data derived from stu...
Influence of diet and water supply on mineral content and pH within the large intestine of horses with enterolithiasis.
Veterinary journal (London, England : 1997)    August 9, 2008   Volume 182, Issue 1 44-49 doi: 10.1016/j.tvjl.2008.05.016
Hassel DM, Spier SJ, Aldridge BM, Watnick M, Argenzio RA, Snyder JR.To determine the effects of two diets and water supplies on intestinal pH and mineral concentrations in the colon of horses, and to identify whether differences in these parameters exist in horses with and without enterolithiasis, surgical fistulation of the right dorsal colon was performed in six adult horses, three with and three without enterolithiasis. Each horse underwent four feeding trials: grass hay and untreated water, alfalfa hay and untreated water, grass hay with filtered/softened water, and alfalfa hay with filtered/softened water. Samples of colonic contents were analyzed for pH,...
Effect of feeding increasing quantities of starch on glycaemic and insulinaemic responses in healthy horses.
Veterinary journal (London, England : 1997)    June 16, 2008   Volume 182, Issue 1 67-72 doi: 10.1016/j.tvjl.2008.04.011
Vervuert I, Voigt K, Hollands T, Cí·¯ord D, Coenen M.The aim of this study was to investigate the effect of increasing the intake of starch on the glycaemic and insulinaemic responses of horses. A cross-over study design was used in which four horses were fed increasing amounts of a compound feed (0.5-3.5 kg) to provide 0.3, 0.6, 0.8, 1.1, 1.4 and 2 starch/kg bodyweight (BW)/meal. The glycaemic response increased with starch intake (P<0.05), while feeding <1.1 g starch/kg BW resulted in a lowered response, compared to when 1.1-2 g starch/ kg BW was fed (P<0.01). The results suggested that insulin responses may be more appropriate to def...
Obstructive urolithiasis, unilateral hydronephrosis, and probable nephrolithiasis in a 12-year-old Clydesdale gelding.
The Canadian veterinary journal = La revue veterinaire canadienne    April 9, 2008   Volume 49, Issue 3 287-290 
Macbeth BJ.A 12-year-old Clydesdale gelding was presented for colic and dysuria. Obstructive urolithiasis and chronic renal disease were diagnosed via transurethral endoscopy and percutaneous ultrasonography. Nephroliths, hydronephrosis, and peri-ureteral fibrosis were present. Surgical intervention was declined and the gelding was managed medically with antibiotics and dietary modification. Urolithiase obstructive, hydronéphrose unilatérale et lithiase rénale probable chez un Clydesdale hongre âgé de 12 ans. Un Clydesdale hongre âgé de 12 ans a été présenté pour colique et dysurie. Une urolit...
Effect of live yeast culture supplementation on apparent digestibility and rate of passage in horses fed a high-fiber or high-starch diet.
Journal of animal science    October 2, 2007   Volume 86, Issue 2 339-347 doi: 10.2527/jas.2006-796
Jouany JP, Gobert J, Medina B, Bertin G, Julliand V.Eight crossbred male horses aged 12 +/- 5 yr and with BW of 305 +/- 18 kg were used in pairs in a 4 x 4 Latin square design with 4 ground and pelleted diets. Each pair included a cecum and right ventral colon-fistulated animal and a cecal-fistulated animal. The 4 horse diets were a high-fiber diet (HF+0) based on dehydrated alfalfa, a high-starch diet based on barley and wheat bran (HS+0), and the HF or HS diets supplemented with Saccharomyces cerevisiae (SC) CBS 493.94 (HF+SC and HS+SC). The probiotic preparation contained 4.5 x 10(9) cfu/g of live yeast mixed with the culture medium, and was...
Etiology and pathogenesis of osteochondrosis.
Veterinary pathology    July 4, 2007   Volume 44, Issue 4 429-448 doi: 10.1354/vp.44-4-429
Ytrehus B, Carlson CS, Ekman S.Osteochondrosis is a common and clinically important joint disorder that occurs in human beings and in multiple animal species, most commonly pigs, horses, and dogs. This disorder is defined as a focal disturbance of enchondral ossification and is regarded as having a multifactorial etiology, with no single factor accounting for all aspects of the disease. The most commonly cited etiologic factors are heredity, rapid growth, anatomic conformation, trauma, and dietary imbalances; however, only heredity and anatomic conformation are well supported by the scientific literature. The way in which t...
Effect of yeast culture on in vitro fermentation of a high-concentrate or high-fiber diet using equine fecal inoculum in a Daisy II incubator.
Journal of animal science    May 25, 2007   Volume 85, Issue 10 2484-2491 doi: 10.2527/jas.2006-655
Lattimer JM, Cooper SR, Freeman DW, Lalman DL.Two experiments were conducted to evaluate the use of a closed system, fermentation apparatus (Daisy II incubator) and determine the effects of a yeast culture (YC) preparation (Saccharomyces cerevisiae) on in vitro microbial populations, diet digestion, and fermentation patterns in horses. In Exp. 1, 4 mature horses were fed a pelleted concentrate and alfalfa cubes in a 50:50 (%, as-fed) ratio. Fecal samples were taken from each horse to form the inoculum and placed in 4 separate incubation vessels. Twenty nylon bags (10 with 0.25 g and 10 with 0.50 g of the total mixed diet) were placed in e...
Effects of dietary energy source and physical conditioning on insulin sensitivity and glucose tolerance in standardbred horses.
Equine veterinary journal. Supplement    April 4, 2007   Issue 36 579-584 doi: 10.1111/j.2042-3306.2006.tb05608.x
Pratt SE, Geor RJ, McCutcheon LJ.There is evidence that adaptation to diets rich in nonstructural carbohydrates (NSC) contributes to the development of insulin resistance in horses. To date, however, no study in horses has examined the effects of physical conditioning on diet-associated alterations in insulin sensitivity and glucose tolerance. Objective: To examine the effects of adaptation to concentrate feeds rich in NSC or fat on insulin sensitivity and glucose tolerance in horses, both in the sedentary state and after a subsequent period of physical conditioning. Methods: Fourteen mature Standardbred horses underwent both...
Lack of effect of diet on the pharmacokinetics of enrofloxacin in horses.
Journal of veterinary pharmacology and therapeutics    January 20, 2006   Volume 29, Issue 1 67-70 doi: 10.1111/j.1365-2885.2006.00717.x
Steinman A, Britzi M, Levi O, Lavy E, Lichter A, Soback S.No abstract available
Effects of rice bran oil on plasma lipid concentrations, lipoprotein composition, and glucose dynamics in mares.
Journal of animal science    October 19, 2005   Volume 83, Issue 11 2509-2518 doi: 10.2527/2005.83112509x
Frank N, Andrews FM, Elliott SB, Lew J, Boston RC.Plasma lipid concentrations, lipoprotein composition, and glucose dynamics were measured and compared between mares fed diets containing added water, corn oil (CO), refined rice bran oil (RR), or crude rice bran oil (CR) to test the hypothesis that rice bran oil lowers plasma lipid concentrations, alters lipoprotein composition, and improves insulin sensitivity in mares. Eight healthy adult mares received a basal diet fed at 1.5 times the DE requirement for maintenance and each of the four treatments according to a repeated 4 x 4 Latin square design consisting of four 5-wk feeding periods. Blo...
Fast food and fat fillies: the ills of western civilisation.
Veterinary journal (London, England : 1997)    September 9, 2005   Volume 172, Issue 3 396-397 doi: 10.1016/j.tvjl.2005.06.009
Sillence M, Noble G, McGowan C.No abstract available
Meal-induced gastric relaxation and emptying in horses after ingestion of high-fat versus high-carbohydrate diets.
American journal of veterinary research    June 7, 2005   Volume 66, Issue 5 897-906 doi: 10.2460/ajvr.2005.66.897
Lorenzo-Figueras M, Preston T, Ott EA, Merritt AM.To evaluate the effect of ingestion of a high-carbohydrate versus a high-fat meal on relaxation of the proximal portion of the stomach and subsequent gastric emptying in horses. Methods: 6 healthy adult horses. Methods: The study consisted of 2 phases. In phase I, horses were offered a high-fat (8% fat) or a high-carbohydrate (3% fat) pelleted meal (0.5 g/kg) of identical volume, caloric density, and protein content. In phase II, meals consisted of a commercial sweet feed meal (0.5 g/kg) or this meal supplemented with corn oil (12.3% fat) or an isocaloric amount of glucose (2.9% fat). Proximal...
Effects of soy isoflavones and conjugated equine estrogens on inflammatory markers in atherosclerotic, ovariectomized monkeys.
The Journal of clinical endocrinology and metabolism    December 7, 2004   Volume 90, Issue 3 1734-1740 doi: 10.1210/jc.2004-0939
Register TC, Cann JA, Kaplan JR, Williams JK, Adams MR, Morgan TM, Anthony MS, Blair RM, Wagner JD, Clarkson TB.The effects of dietary soy isoflavones (IF) and conjugated equine estrogens (CEE) on circulating inflammatory markers were determined at the end of a 3-yr study of ovariectomized monkeys consuming a moderately atherogenic diet. Treatments were: 1) control, receiving alcohol-extracted soy-protein-based diet with low IF content (comparable to approximately 5 mg/d); 2) CEE, added to the control diet at a dose comparable to 0.625 mg/d; and 3) IF, consumed as a part of unextracted soy protein isolate at a dose comparable to 129 mg/d. Serum soluble vascular cell adhesion molecule-1 (sVCAM-1) was red...
Effect of diet composition and feeding pattern on the prececal digestibility of starches from diverse botanical origins measured with the mobile nylon bag technique in horses.
Journal of animal science    November 13, 2004   Volume 82, Issue 12 3625-3634 doi: 10.2527/2004.82123625x
de Fombelle A, Veiga L, Drogoul C, Julliand V.This trial was conducted to determine the extent of prececal starch digestibility depending on the botanical origin of starch and on diet characteristics (i.e., composition and feeding pattern). The prececal disappearance of six substrates (oats, barley, corn, horse bean, potato, and wheat) was measured in four cannulated horses fed (as-fed basis) 11.8 g/kg BW of a high-fiber (HF) or high-starch (HS) pelleted feed and 10.0 g/kg BW of meadow hay using the mobile bag technique (MBT). The daily feeding pattern was either three meals (two meals of pellets and one meal of hay) or five meals (three ...
Effect of hypothyroidism on the blood lipid response to higher dietary fat intake in mares.
Journal of animal science    September 28, 2004   Volume 82, Issue 9 2640-2646 doi: 10.2527/2004.8292640x
Frank N, Sojka JE, Latour MA.Blood lipid and lipoprotein concentrations were measured and compared between euthyroid and thyroidectomized mares on low-fat or high-fat diets to test the hypothesis that hypothyroidism alters the blood lipid response to higher dietary fat intake. Four healthy adult mares and four adult mares that had been thyroidectomized 3 to 6 mo earlier were placed on low-fat or high-fat diets according to a replicated 2 x 2 Latin square design consisting of two 5-wk feeding periods separated by a 2-wk washout interval. Plasma lipid concentrations were measured at 0, 3, 4, and 5 wk, and plasma lipase acti...
Macronutrient digestibility, nitrogen balance, plasma indicators of protein metabolism and mineral absorption in horses fed a ration rich in sugar beet pulp.
Journal of animal physiology and animal nutrition    September 25, 2004   Volume 88, Issue 9-10 321-331 doi: 10.1111/j.1439-0396.2004.00487.x
Olsman AF, Huurdeman CM, Jansen WL, Haaksma J, Sloet van Oldruitenborgh-Oosterbaan MM, Beynen AC.In a cross-over study with six mature horses, the effect of iso-energetic replacement of dietary glucose by beet pulp on macronutrient digestibility, nitrogen metabolism and mineral absorption was studied. The test ration contained 25% beet pulp in the total dietary dry matter. Beet pulp feeding significantly lowered crude fat and non-structural carbohydrate digestibility, but had no significant effect on digestibility of other macronutrients, faecal and urinary nitrogen excretion and the faecal to urinary nitrogen excretion quotient. However, on the beet pulp diet, plasma ammonia and creatini...
Effects of feeding meals with various soluble-carbohydrate content on muscle glycogen synthesis after exercise in horses.
American journal of veterinary research    July 30, 2004   Volume 65, Issue 7 916-923 doi: 10.2460/ajvr.2004.65.916
Lacombe VA, Hinchcliff KW, Kohn CW, Devor ST, Taylor LE.To determine effects of feeding diets with various soluble-carbohydrate (CHO) content on rates of muscle glycogen synthesis after exercise in horses. Methods: 7 fit horses. Methods: In a 3-way crossover study, horses received each of 3 isocaloric diets (a high soluble CHO [HC] diet, a low soluble CHO [LC] diet, or a mixed soluble CHO [MC] diet). For each diet, horses were subjected to glycogen-depleting exercise, followed by feeding of the HC, LC, or MC diet at 8-hour intervals for 72 hours. Results: Feeding the HC diet resulted in a significantly higher glycemic response for 72 hours and sign...
Soybean hulls as an alternative feed for horses.
Journal of animal science    June 26, 2004   Volume 82, Issue 6 1663-1668 doi: 10.2527/2004.8261663x
Coverdale JA, Moore JA, Tyler HD, Miller-Auwerda PA.Soybean hulls have been successfully fed to ruminant animals as an economical substitute for hay. This feedstuff is a source of highly digestible fiber that does not contain starch. The purpose of this trial was to evaluate soybean hulls as a replacement fiber in horse diets. Four cecally cannulated Quarter Horse geldings, aged 6 to 10 yr and averaging 502 kg, were used in a 4 x 4 Latin square design with 21-d periods. Diets consisted of alfalfa/bromegrass hay (14.4% CP, 58.1% NDF, 39.1% ADF; DM basis) with the replacement of either 0, 25, 50, or 75% (as-fed basis) unpelleted soybean hulls (13...
Epidemiologic characteristics and management of polysaccharide storage myopathy in Quarter Horses.
American journal of veterinary research    November 5, 2003   Volume 64, Issue 10 1319-1327 doi: 10.2460/ajvr.2003.64.1319
Firshman AM, Valberg SJ, Bender JB, Finno CJ.To characterize onset and clinical signs of polysaccharide storage myopathy (PSSM) in a well-defined population of affected Quarter Horses, identify risk factors for PSSM, determine compliance of owners to dietary and exercise recommendations, and evaluate the efficacy of dietary and exercise recommendations. Methods: 40 Quarter Horses with PSSM and 37 unaffected control horses. Methods: Owners of horses with PSSM completed a retrospective questionnaire concerning their horse's condition. Results: Between horses with PSSM and control horses, no significant differences were found in sex distrib...
Serum beta-carotene and alpha-tocopherol in horses fed beta-carotene via grass-meal or a synthetic beadlet preparation with and without added dietary fat.
Journal of animal physiology and animal nutrition    September 27, 2003   Volume 87, Issue 3-4 174-180 doi: 10.1046/j.1439-0396.2003.00430.x
Kienzle E, Kaden C, Hoppe PP, Opitz B.The serum response of beta-carotene as an indicator of bioavailability was compared after feeding beta-carotene (0.8 mg/kg body weight) either from grass meal or a synthetic beadlet preparation (Lucarotin). Both were each given without or with added dietary vegetable fat (2-2.5% vs. 6.6% fat in dry matter) in a Latin square design with four horses. The nutritionally complete diet was supplemented with alpha-tocopherol (4 mg/kg body weight). Each treatment period (4 weeks, two serum samples) was followed by a washout period of 4 weeks with low intakes of beta-carotene (traces) and alpha-tocophe...
Differential mesowear in occluding upper and lower molars: opening mesowear analysis for lower molars and premolars in hypsodont horses.
Journal of morphology    August 9, 2003   Volume 258, Issue 1 67-83 doi: 10.1002/jmor.10125
Kaiser TM, Fortelius M.A new approach of reconstructing ungulate diet, the mesowear method, was recently introduced by Fortelius and Solounias ([2000] Am Mus Novitat 3301:1-36). Mesowear is based on facet development on the occlusal surfaces of the teeth. Restricting mesowear investigation to maxillary cheek teeth would allow mesowear investigation only in assemblages of large numbers of individuals and therefore would generally restrict this method to relatively few assemblages of recent and fossil ungulates. Most of the fossil, subfossil, and recent ungulate osteological assemblages that may be assigned to a singl...
Production and clearance of plasma triacylglycerols in ponies fed diets containing either medium-chain triacylglycerols or soya bean oil.
Journal of animal physiology and animal nutrition    May 20, 2003   Volume 87, Issue 5-6 188-195 doi: 10.1046/j.1439-0396.2003.00395.x
Hallebeek JM, Beynen AC.The hypothesis was tested that feeding ponies a diet containing medium-chain triacylglcyerols (MCT) instead of soya bean oil causes an increase in the production of plasma triacylglycerols, which, under steady-state conditions, is associated with an increased clearance of triacylglycerols. Six ponies were fed rations containing either MCT or an isoenergetic amount of soya bean oil according to a cross-over design. The concentration of MCT in the total dietary dry matter was about 13%. When the ponies were fed the diets for 3 weeks, plasma triacylglycerol concentrations were 0.42 +/- 0.09 and 0...
Comparison of volumetric urine collection versus single-sample urine collection in horses consuming diets varying in cation-anion balance.
American journal of veterinary research    March 29, 2003   Volume 64, Issue 3 284-291 doi: 10.2460/ajvr.2003.64.284
McKenzie EC, Valberg SJ, Godden SM, Pagan JD, Carlson GP, MacLeay JM, DeLaCorte FD.To determine daily variation in urinary clearance and fractional excretion (FE) of electrolytes and minerals within and between horses and to compare volumetric and single-sample urine collection for determining FE values of diets with a range of dietary cation-anion balance (DCAB). Methods: 5 Thoroughbred and 6 mixed-breed mares. Methods: 3 isocaloric diets with low, medium, and high DCAB values (85, 190, and 380 mEq/kg of dry matter, respectively) were each fed for 14 days. Daily blood samples, single urine samples collected by using a urinary catheter (5 mares), and volumetric urine collect...
Nutritional responses to the presence of inulin and oligofructose in the diets of domesticated animals: a review.
Critical reviews in food science and nutrition    February 18, 2003   Volume 43, Issue 1 19-60 doi: 10.1080/10408690390826446
Flickinger EA, Van Loo J, Fahey GC.Inulin and oligofructose are prebiotic oligosaccharides fermented in the large intestine. This article provides an extensive review of the effects of these oligosaccharides on gastrointestinal characteristics (microflora, pathogen control, epithelial cell proliferation, putrefactive compound production, fecal characteristics, and nutrient digestibility) and systemic metabolism of carbohydrates, nitrogen, lipids, and minerals in dogs, cats, horses, calves, pigs, poultry, and rabbits. In addition, intake of inulin and oligofructose and considerations in their supplementation to animal diets are ...
Effect of a preparation of Saccharomyces cerevisiae on microbial profiles and fermentation patterns in the large intestine of horses fed a high fiber or a high starch diet.
Journal of animal science    November 5, 2002   Volume 80, Issue 10 2600-2609 doi: 10.2527/2002.80102600x
Medina B, Girard ID, Jacotot E, Julliand V.Eight horses were allotted into pairs consisting of one cecum- and right ventral colon-fistulated animal and one cecum-fistulated animal. They were fed daily at the same level of intake either a high-fiber (HF) or a high-starch (HS) diet without or with 10 g of a Saccharomyces cerevisiae preparation, in a 4 x 4 Latin square design. The HS diet provided a starch overload (i.e., 3.4 g starch x kg(-1) BW x meal(-1)) while maintaining a high amount of fiber intake (i.e., dietary NDF/starch ratio was 1.0). A 21-d period of adaptation to the treatments occurred before cecal and colonic contents were...
Exercise- and metabolism-associated blood variables in Standardbreds fed either a low- or a high-fat diet.
Equine veterinary journal. Supplement    October 31, 2002   Issue 34 29-32 doi: 10.1111/j.2042-3306.2002.tb05387.x
Sloet van Oldruitenborgh-Oosterbaan MM, Annee MP, Verdegaal EJ, Lemmens AG, Beynen AC.Feeding a high-fat diet increases fat utilisation and may decrease glycogen utilisation resulting in a lower lactate production during moderate exercise. The effects of fat feeding on exercise- and lipid metabolism-associated blood variables were evaluated in 6 Standardbred horses during submaximal exercise. The horses were fed a high- (11.8% fat in total dietary dry matter) or a low-fat diet (1.5% fat) in a cross-over experiment with feeding periods of 4 weeks. At the end of each feeding period, the horses were subjected to a submaximal standardised stepwise exercise test on a treadmill. Bloo...