Equids are members of the family Equidae, which includes modern horses, donkeys, and zebras, as well as extinct species. Equids are characterized by their long limbs, single-toed hooves, and herbivorous diet, which is primarily composed of grasses. Horses, specifically, have been domesticated for thousands of years and have played significant roles in agriculture, transportation, and sport. This page compiles peer-reviewed research studies and scholarly articles that explore various aspects of equids and horses, including their evolution, physiology, behavior, and interactions with humans. The collected works provide insights into the genetic diversity, adaptive traits, and conservation efforts related to equid species.
Sigurðardóttir H, Ablondi M, Kristjansson T, Lindgren G, Eriksson S.The Icelandic horse and Exmoor pony are ancient, native breeds, adapted to harsh environmental conditions and they have both undergone severe historic bottlenecks. However, in modern days, the selection pressures on these breeds differ substantially. The aim of this study was to assess genetic diversity in both breeds through expected (HE) and observed heterozygosity (HO) and effective population size (Ne). Furthermore, we aimed to identify runs of homozygosity (ROH) to estimate and compare genomic inbreeding and signatures of selection in the breeds. Results: HO was estimated at 0.34 and 0.33...
Leng J, Moller-Levet C, Mansergh RI, O'Flaherty R, Cooke R, Sells P, Pinkham C, Pynn O, Smith C, Wise Z, Ellis R, Couto Alves A, La Ragione R....Gut bacterial communities have a profound influence on the health of humans and animals. Early-life gut microbial community structure influences the development of immunological competence and susceptibility to disease. For the Thoroughbred racehorse, the significance of early-life microbial colonisation events on subsequent health and athletic performance is unknown. Here we present data from a three-year cohort study of horses bred for racing designed to explore interactions between early-life gut bacterial community structure, health events in later life and athletic performance on the race...
Michanek P, Bröjer J, Lilliehöök I, Fjordbakk CT, Löwgren M, Hedeland M, Bergquist J, Ekstrand C.Canagliflozin (CFZ) is a sodium-glucose cotransporter-2 inhibitor that has shown promising results as a drug for the treatment of insulin dysregulation in horses. Even though CFZ is used clinically, no pharmacokinetic data has previously been published. In this study, the pharmacokinetics of CFZ after administration of a single oral dose of 1.8 mg/kg in eight healthy Icelandic horses was examined. Additionally, the effect of treatment on glucose and insulin levels in response to a graded glucose infusion was investigated. Plasma samples for CFZ quantification were taken at 0, 0.33, 0.66, 1, ...
Bermukhametov Z, Suleimanova K, Tomaruk O, Baimenov B, Shevchenko P, Batyrbekov A, Mikniene Z, Onur Girişgin A, Rychshanova R.A total of 396 samples were taken from the hearts, oesophagi, and diaphragms of 132 horses slaughtered at slaughterhouses in 2023 for subsequent examination. Methods: The histological method revealed pathomorphological changes in the muscle tissue. The molecular method identified the pathogen species. Results: Histological examination revealed thick-walled cysts with internal septa and numerous bradyzoites, and mononuclear inflammatory cells with pericyst infiltrates. Microcyst samples were amplified by polymerase chain reaction. Molecular genetic analysis allowed for the identification of 18 ...
Szkolnicka K, Mituniewicz-Małek A, Dmytrów I, Bogusławska-Wąs E.During the last years, growing interest in the use of mare's milk in food production is observed. The subject of the study was to evaluate the feasibility of mare's milk for the production of yogurt ice cream and synbiotic ice cream. Four variants of mare's milk ice cream were developed: ice cream with yogurt bacteria without inulin (YO) and with 2% of inulin (YO+I), synbiotic ice cream with 2% inulin and Lacticaseibacillus rhamnosus (LCR+I) and with Lactiplantibacillus plantarum (LP+I). Ice creams were enriched with inulin in order to evaluate its influence on the viability of LAB and on the ...
Woo PCY, Mheiri FA, Cavalleri J, Joseph S, Tang JYM, Joseph M, Tsang CC, Lau SKP, Wernery U.Epizootic lymphangitis (EL) is a highly prevalent and contagious infectious disease affecting horses in many parts of Ethiopia caused by Histoplasma capsulatum sensu lato ('var. farciminosum'). In this study, 12 suspected isolates of H. capsulatum sensu lato or yeasts unidentified by conventional biochemical tests isolated from Ethiopian horses with EL were characterised by ITS sequencing. Six of the 12 isolates were identified to be members of H. capsulatum sensu lato and the other six were Pichia kudriavzevii (synonym: Candida krusei) (n = 3), Trichosporon asahii (n = 1), Geotrichum silv...
Yaneselli K, Ávila G, Rossi A, Rial A, Castro S, Estradé MJ, Suárez G, Algorta A.Platelet lysate (PL) is investigated as a potential replacement for fetal bovine serum (FBS) in cell culture. However, there is limited research on its impact on the immune profile of equine mesenchymal stromal cells (eMSCs). This study aimed to evaluate the effects of different PL formulations on the proliferative capacity, multipotentiality, and immune profile of equine adipose tissue-derived MSCs (eAD-MSCs). growth kinetics and trilineage differentiation of eAD-MSCs ( = 7) were assessed under three culture conditions: medium-concentration PL (MPL), high-concentration PL (HPL), and FBS ...
Octaviano JI, Alonso MA, Boakari YL, Gomes V, Mori CS, Fleury PDC, Fernandes CB.Monitoring equine parturition effectively is essential for preemptive intervention in periparturient issues and ensuring the overall well-being of both mares and foals. However, its implementation in breeding farms is challenging due to variable gestational lengths and nocturnal births. Predictive techniques have the potential to streamline the monitoring process, reduce labor intensity, and minimize costs. Research on foaling prediction in mares carrying mule or equine clone fetuses is scarce. Therefore, this study aimed to comparatively analyze foaling prediction parameters in mares pregnant...
Lawson JM, Salem SE, Miller D, Kahler A, van den Boer WJ, Shilton CA, Sever T, Mouncey RR, Ward J, Hampshire DJ, Foote AK, Bryan JS, Juras R, Pynn OD....Chromosomal abnormalities are a common cause of human miscarriage but rarely reported in any other species. As a result, there are currently inadequate animal models available to study this condition. Horses present one potential model since mares receive intense gynecological care. This allowed us to investigate the prevalence of chromosomal copy number aberrations in 256 products of conception (POC) in a naturally occurring model of pregnancy loss (PL). Triploidy (three haploid sets of chromosomes) was the most common aberration, found in 42% of POCs following PL over the embryonic period. O...
Poochipakorn C, Wonghanchao T, Sanigavatee K, Chanda M.Single-confinement housing can pose welfare risks to domestic horses. This study investigated horses' stress responses when confined to single stalls in different stable designs in a tropical savanna region to address a gap in the literature. In total, 23 horses were assigned to a stable with a central corridor and solid external walls (A) (N = 8), a stable with one side corridor and solid external walls (B) (N = 6), or a stable with a central corridor and no solid external walls (C) (N = 9). Air velocity, relative humidity, air temperature, and noxious gases were measured inside the stables, ...
Richards S, Palmer D, Cawley A, Wainscott M, Keledjian J.An improved screening workflow and a robust capillary flow LC-MS confirmatory method for the detection of recombinant human erythropoietin (rHuEPO) has been implemented to increase the sensitivity of rHuEPO detection and to reduce the number of suspect samples committed to confirmatory testing. The influence of repeated dosing of epoetin-β on the detection window of rHuEPO in equine plasma was assessed using the optimised method. Samples were initially assessed using an economical R&D Human EPO Duo-Set ELISA Development System. Samples indicating a result greater than the batch baseline were ...
White NA, Flynn K.The purpose of the study was to assess the challenges faced by state animal health officials (SAHOs) when managing equine disease outbreaks. Methods: A survey was conducted to assess SAHOs' collaboration with horse owners and practicing veterinarians to provide biosecurity and infection disease control. It was sent to 180 email addresses listed in the Equine Disease Communication Center website database. The responses in each category for each question were tabulated and reported as a percentage. Results: There were 72 survey responses from 45 states. The number of states responding suggested ...
Okudaira M, Cresswell EN, Wollman CW, McDonough SP, Engiles JB, Reesink HL.To describe the development and maturation of equine proximal sesamoid bones (PSBs) in fetuses and young horses using radiography, microcomputed (micro)-CT, and histology. Methods: A descriptive study. Forelimb PSBs from 12 equids ranging in age from 105 days of gestation to 540 days postgestation were evaluated. Radiography was used for preliminary assessment of metacarpophalangeal joint and PSB mineralization, and micro-CT imaging was performed to assess mineralized PSBs. Tissue volume, bone volume fraction, height, width, depth, trabecular thickness, and anisotropy were quantified from midp...
Duderstadt S, Distl O.The objective of the present study was to analyze the genetic diversity, individual-based assessment of population structure, and admixture in the Dülmen wild horse population in comparison to warmblood, coldblood, and primitive horse populations. The Dülmen wild horse is kept as a unique horse population in the Merfelder Bruch near Dülmen in Westphalia, Germany, and since 1856 has been managed by the Dukes of Croÿ. The Dülmen wild horse population is exposed to the natural conditions of the Merfelder Bruch all year round without human interventions for feeding and veterinary care. In the...
Giontella A, Silvestrelli M, Cocciolone A, Pieramati C, Sarti FM.Morphological scoring is a common evaluation method for domestic animals. The National Association of Maremmano Breeders (ANAM) has provided a dataset containing the records of 600 horses, four metric measurements (cm) and 24 traits with a continuous evaluation scale, each one with 15 classes. Moreover, a body condition score (BCS) with five classes is included. In this study, factor analysis was conducted to create a small number of informative factors (3) obtained from these traits, and a new BLUP-AM-MT index was established. The New Estimated Breeding Value (NEBV1) of each horse was compute...
Ren H, Wen X, He Q, Yi M, Dugarjaviin M, Bou G.The reproductive performance of horse sperm and donkey sperm has been reported to differ. Sperm proteins play a crucial role in sperm viability and fertility. Although differences between species are known, no prior study has investigated disparities in the sperm proteome between horses and donkeys. Therefore, this study characterized and compared the sperm proteomes of horses and donkeys using 4D-DIA mass spectrometry technology. We identified 3436 proteins in horse sperm and 3404 proteins in donkey sperm. Of these, 3363 proteins were expressed in both horse and donkey sperm, with 73 proteins...
Doyle JL, Egan S, Berry DP.Many sport horse studbooks worldwide use microsatellite markers for parentage verification. However, many have expressed a desire to introduce genomic selection using genome-wide dense single nucleotide polymorphism (SNP) genotypes to complement their current breeding programmes. Hence, it does not make sense to genotype the same animal for both microsatellite markers and SNP markers. Transitioning to SNP-based parentage verification is an obvious solution but one barrier to this transition is the lack of SNP data on parents from which to verify parentage against. Therefore, the objective of t...
Jardat P, Menard-Peroy Z, Parias C, Reigner F, Calandreau L, Lansade L.Recently, horses and other domestic mammals have been shown to perceive and react to human emotional signals, with most studies focusing on joy and anger. In this study, we tested whether horses can learn to identify human joyful and sad expressions against other emotions. We used a touchscreen-based automated device that presented pairs of human portraits and distributed pellets when the horse touched the rewarded face. Six horses were trained to touch the sad face and 5 the joyful face. By the end of training, horses' performances at the group level were significantly higher than chance leve...
Calomeno SS, Freitas Santi T, Barbosa B, Weber SH, Oliveira TM, Machado GF, Michelotto PV.Horses maintained outdoors may experience a lower-allergenic environment compared to their stabled counterparts. This study hypothesizes that climatic changes in southern Brazil can influence respiratory status. To test this hypothesis, we evaluated the lower airways of 17 horses from a teaching herd in southern Brazil, maintained outdoors, during winter, spring, and summer. Except for one mare with a history of severe asthma, all horses were considered healthy and underwent a physical examination. Airway endoscopic evaluation included scoring for tracheal mucus (0-5) and bronchial septum thic...
Maeda M, Abe M, Aoshima K, Kobayashi A, Fukushi H, Kimura T.Equine herpesvirus-1 (EHV-1) causes respiratory diseases, abortion, and encephalomyelitis in horses. The EHV-1 immediate-early (IE) protein, essential for viral replication, is transactivated by the binding of a multiprotein complex including the open reading frame 12 (ORF12) and some host factors to the IE promoter region. Promoter-associated non-coding RNAs (pancRNAs), which are transcribed from bidirectional promoters, regulate the transcription of neighboring genes in mammals and pathogens. In this study, we identified a novel pancRNA transcribed from across the areas of the 5'-untranslate...
Skedros JG, Dayton MR, Cronin JT, Mears CS, Bloebaum RD, Wang X, Bachus KN.Many bones experience bending, placing one side in net compression and the other in net tension. Because bone mechanical properties are relatively reduced in tension compared with compression, adaptations are needed to reduce fracture risk. Several toughening mechanisms exist in bone, yet little is known of the influences of secondary osteon collagen/lamellar 'morphotypes' and potential interplay with intermolecular collagen cross-links (CCLs) in prevalent/predominant tension- and compression-loaded regions. Paired third metacarpals (MC3s) from 10 adult horses were prepared for mechanical test...
Grzyb J, Podstawski Z, Bulski K.Horses stay in different types of stables; especially during the cold season, they stay inside for most of the day. A stable is also a place where many people spend quite a lot of time either as employees who care for and train horses or as equine enthusiasts. Keeping horses in stables causes their constant exposure to high concentrations of particulate matter (PM) and molds in the air inside these facilities. The study was conducted in Udórz Stud Farm located in the southern region of Poland. It was carried out in two different types of stables: three runners and two box stables. The study c...
Shams F, Jokar M, Abdous A, Mohammadi P, Abbassioun A, Seuberlich T, Rahmanian V.Neospora spp. and Toxoplasma gondii are two closely related protozoan parasites that are widely distributed throughout the world. Horses can act as intermediate hosts for both parasites and can acquire disease. Blood samples were taken from 487 clinically healthy horses from 17 different mechanized stables in Tehran, the capital of Iran, during September and November of 2022. IFAT and ELISA were employed to detect antibodies directed against Neospora spp. and T. gondii. The anti-N. caninum antibodies were detected in 52 of the horses (10.67%) based on IFAT and in 86 of the 487 horses (17.65%) ...
Nowak M, Martin-Cirera A, Jenner F, Auer U.Pain assessment in horses presents a significant challenge due to their nonverbal nature and their tendency to conceal signs of discomfort in the presence of potential threats, including humans. Therefore, this study aimed to identify pain-associated behaviors amenable to automated AI-based detection in video recordings. Additionally, it sought to determine correlations between pain intensity and behavioral and postural parameters by analyzing factors such as time budgets, weight shifting, and unstable resting. The ultimate goal is to facilitate the development of AI-based quantitative tools f...
Stothart MR, McLoughlin PD, Medill SA, Greuel RJ, Wilson AJ, Poissant J.Gut microbiomes are widely hypothesised to influence host fitness and have been experimentally shown to affect host health and phenotypes under laboratory conditions. However, the extent to which they do so in free-living animal populations and the proximate mechanisms involved remain open questions. In this study, using long-term, individual-based life history and shallow shotgun metagenomic sequencing data (2394 fecal samples from 794 individuals collected between 2013-2019), we quantify relationships between gut microbiome variation and survival in a feral population of horses under natural...
Data in briefJuly 22, 2024
Volume 55 110764 doi: 10.1016/j.dib.2024.110764
Gmel AI, Haraldsdóttir EH, Serra-Bragança F, Lamas LP, Rosa TV, Stefaniuk-Szmukier M, Klecel W, Neuditschko M, Weishaupt MA.Horses have been used and bred for centuries for their movements. However, specific breeds are expected to have different movement capabilities. We have measured 425 horses from four different breeds at walk and trot on a straight line using an inertial measurement unit (IMU) system (EquiMoves®). This article describes how the data was collected, filtered and analysed to provide a useable dataset of 28 movement variables. It provides a full protocol for field measurements and requirements for adequate trials for analysis. Intra-class correlation coefficient estimates are also provided to asse...
Martin F, Halvarsson P, Alm YH, Tydén E.Anthelmintic resistance is an increasing problem in many gastrointestinal parasites of grazing animals. Among these, the equine roundworm, Parascaris spp., has developed wide-spread resistance to macrocyclic lactones over the past decades. Additionally, there are recent observations of emerging treatment failure of both tetrahydropyrimidine and fenbendazole. Therefore, the aims of this study were to further investigate the occurrence of fenbendazole resistance on breeding farms and to explore potential management-related risk factors associated with resistance in Parascaris spp. in Sweden. Ele...
Fanelli D, Moroni R, Sala G, Melanie P, Tarabella I, Telleschi N, Maltinti S, Giorgi M, Barsotti G, Passamonti F, Marmorini P, Rota A, Camillo F....Two studies were conducted to evaluate the use of medical ozone (O) in commercial extenders for equine semen cryopreservation. In the first study (Study 1), 0, 5, and 15 µg/mL of O were added to diluents of refrigerated or frozen semen. Samples were evaluated for sperm kinematics at different time points for the chilled samples and after a thermoresistence test for the frozen/thawed samples. In the second study (Study 2), 0, 5, and 10 µg/mL of O were added to an antibiotic-free diluent for refrigerated semen for comparison with the control group in which semen was diluted in the same diluent...
Marques CDP, da Silva BWS, Nogueira YVS, Bezerra TL, Borges-Silva W, Soares RM, Gondim LFP.Sarcocystis bertrami (synonym: Sarcocystis fayeri) is a coccidian parasite that infects horses and donkeys in several countries. Dogs are known as definitive hosts of the parasite, however, the patent period is not well defined, and S. bertrami shed by dogs has never been confirmed by molecular methods. Here we investigated the shedding of S. bertrami by experimentally infected dogs and examined the excreted parasites by morphological and molecular tools. Three dogs of small breeds (one Yorkshire terrier and two miniature Pinschers) were acquired with ages of 30 and 60 days and were exclusivel...
Seck M, Carder G, Wathan J, Randau M, Fletcher K, Proops L.The role that working equids play in both rural and urban communities in low and middle-income countries is invaluable. They contribute to daily tasks such as carrying food, water and people, support income generation, and are of social and cultural importance. Despite their importance, global standards of working equid welfare are low. Many variables can impact the welfare status of animals under human care, but often specific factors are explored in isolation. Factors can include, but are not limited to an owner's socioeconomic status and their attitudes and beliefs towards animals. In this ...
Huangsaksri O, Wonghanchao T, Sanigavatee K, Poochipakorn C, Sukhong P, Chanda M.Lunging is a training technique commonly performed in a round pen or on a lunge line. This study investigated the effort intensity, autonomic responses, and method agreement in applying different lunging protocols to untrained ponies. Sixteen untrained ponies (aged 13.6 ± 6.3 years and weighing 358 ± 47.4 kg) were studied. Each pony underwent the same research-focused lunging program on a lunge line and, subsequently, in a round pen with a two-day interval between sessions. The heart rate variability (HRV) and effort intensity, indicated as a percentage of maximum heart rate (%HRmax), were d...
Share ER, Mastellar SL, Suagee-Bedore JK, Eastridge ML.The measurement of fecal cortisol/corticosterone metabolites (FCMs) is often used to quantify the stress response. The sampling method is relatively non-invasive, reduces concern for elevation of cortisol from the sampling method, and has been shown to measure cortisol more consistently without the daily diurnal rhythm observed in blood. Commercial ELISA (enzyme-linked immunoassay) kits offer benefits over previously validated immunoassay methods but lack validation. The objective of this study was to evaluate a commercial ELISA kit (Arbor Assays DetectX Cortisol ELISA kit, K003-H1, Ann Arbor,...
Steuer A, Fritzler J, Boggan S, Daniel I, Cowles B, Penn C, Goldstein R, Lin D.Current methods for obtaining fecal egg counts in horses are often inaccurate and variable depending on the analyst's skill and experience. Automated digital scanning of fecal sample slides integrated with analysis by an artificial intelligence (AI) algorithm is a viable, emerging alternative that can mitigate operator variation compared to conventional methods in companion animal fecal parasite diagnostics. Vetscan Imagyst is a novel fecal parasite detection system that uploads the scanned image to the cloud where proprietary software analyzes captured images for diagnostic recognition by a d...
Li S, Li L, Sun Y, Khan MZ, Yu Y, Ruan L, Chen L, Zhao J, Jia J, Li Y, Wang C, Wang T.Equid herpesvirus type 8 (EqHV-8) is known to cause respiratory disease and miscarriage in horses and donkeys, which is a major problem for the equine farming industry. However, there are currently limited vaccines or drugs available to effectively treat EqHV-8 infection. Therefore, it is crucial to develop new antiviral approaches to prevent potential pandemics caused by EqHV-8. This study evaluates the antiviral and antioxidant effects of cepharanthine against EqHV-8 by employing both in vitro assays and in vivo mouse models to assess its therapeutic efficacy. To assess the effectiveness of ...
Thomson P, Garrido D, Santibáñez R, Lara F.The gut microbiome plays a critical role in maintaining horse health, influencing digestion, immunity, and overall well-being. However, in certain conditions like colic, there is evidence of significant alterations in the microbial community. To analyze the composition of the fecal microbiome and the enriched predicted metabolic functions of horses with colic compared to a control group, 14 horses with colic and 14 control horses were recruited. From a stool sample, DNA extraction was carried out for subsequent 16S rRNA metagenomic analysis. The composition of the microbiome was analyzed from ...
Martin-Pelaez S, Fuente A, Takahashi K, Perez IT, Orozco J, Okada CTC, Neto CR, Meyers S, Dini P.Intracytoplasmic sperm injection (ICSI) is the current clinical practice for the in vitro production of equine embryos. The use of conventional fertilization methods such as in vitro fertilization (IVF), has historically been associated with poor success in horses. However, recent improvements have led to better outcomes with IVF, though only when using fresh semen, which limits its use in clinical practice. IVF remains in its infancy in equine reproduction, and several unknowns remain about the technique. One significant gap in knowledge concerns the morphokinetics of IVF embryos and how they...
Asti V, Ablondi M, Molle A, Zanotti A, Vasini M, Sabbioni A.The shift of the horse breeding sector from agricultural to leisure and sports purposes led to a decrease in local breeds' population size due to the loss of their original breeding purposes. Most of the Italian breeds must adapt to modern market demands, and gait traits are suitable phenotypes to help this process. Inertial measurement unit (IMU) technology can be used to objectively assess them. This work aims to investigate on IMU recorded data (i) the influence of environmental factors and biometric measurements, (ii) their repeatability, (iii) the correlation with judge evaluations, and (...
Hall C, Kay R.Quality of life is dependent upon the extent to which behavioural needs are met, and the balance between pleasant and unpleasant lifetime experiences. In Part II of this systematic review, articles (n = 109) relating to horse-human interactions were reviewed to identify behavioural evidence of their positive or negative impact on the horse. The number of articles (n = 22) relating to the recognition of pain in horses, indicated the importance of identifying health issues, which are also likely to increase the aversiveness of interactions. These and articles relating to emotional reactivity tes...
Tasnim Y, Stanley C, Rahman MK, Awosile B.We aim to detect beta-lactamase-producing Citrobacter sedlakii from horses and compare the genomic characteristics with isolates from humans. Results: We characterized phenotypically and genotypically nine C. sedlakii isolates from the feces of horses and then compared them to human-derived isolates using whole genome sequencing and phylogenomic methods. Seven isolates (7/9) were ampicillin-resistant, while at least one isolate was resistant to ceftriaxone, gentamicin, meropenem, and streptomycin. All nine isolates were carriers of the chromosomal-mediated blaSED-1 beta-lactamase gene, which...
Ahlawat S, Sharma U, Niranjan SK, Chhabra P, Arora R, Sharma R, Singh KV, Vijh RK, Mehta SC.This study explored the maternal genetic diversity of six indigenous Indian horse and pony breeds (Bhutia, Kathiawari, Manipuri, Marwari, Spiti, and Zanskari) using comprehensive mitochondrial genome (mitogenome) analysis. Blood samples from 53 horses across diverse agro-climatic zones of India were analyzed, revealing 36 distinct haplotypes, with a haplotype diversity of 0.889 and nucleotide diversity of 0.00347. These indices suggest significant maternal genetic diversity in Indian equines. A median-joining (MJ) network, based on the hypervariable region of the D-loop along with sequences of...
Nassar AFC, Chiebao DP, Fava CD, Miyashiro S, Castro V, Ogata RA, Yamamora JM, Monteiro CAS, Monteiro EJB.Glanders is a zoonotic disease of equids caused by the bacterium Burkholderia mallei, responsible for considerable economic loss. This study aimed to describe the clinical manifestations, pathological findings, and also bacteriological and molecular methods for agent detection in naturally infected animals (16 adult horses and one fetus) detected by serological survey from three glanders outbreaks. Of the 16 horses, 6 (37.5%) did not show clinical signs. After necropsy,samples of organs, lymph nodes, lesions and secretions were collectedfor histopathology, bacterial isolation, and PCR. The cli...
The aim of this study was to evaluate the effects of two styles of classical music, based on different tempos (BPM), on the physiological and blood parameters of horses during social isolation and restriction of movements. First experiment was carried out using nine horses of no defined breed, distributed in Control, Slow-tempo music and Moderate-tempo music .For social isolation and restriction of movement, the animals were housed daily in individual stalls for two hours and exposed to the stimuli for 60 min, and eye temperature, heart rate, and respiratory rate were assessed. The second exp...
Baghdadi HBA, Abdelsalam M, Attia MM.Strongylus vulgaris, a devastating parasitic nematode in equids, causes life-threatening verminous aneurysms that are challenging to diagnose early. This study pioneered integrating nanotechnology into an indirect enzyme-linked immunosorbent assay (i-ELISA) system to enhance the sensitivity and specificity for detecting S. vulgaris larval antigens in equine serum samples, with PCR confirmation of the species. A conventional i-ELISA and an innovative nano-based ELISA were developed using excretory-secretory antigens from adult S. vulgaris worms. The nano-ELISA incorporated gold nanoparticles (1...
Torcivia C, McDonnell SM.Gastric ulcer disease and other potentially painful gastric conditions are among the most common afflictions adversely affecting the welfare of domestic equids. A large percentage of affected animals may not display the classic signs of gastric disease, such as unexplained weight loss, poor hair coat, and inappetence until the disease becomes severe. As a clinical service within our equine referral hospital, we routinely evaluate 24-h video recorded samples of horses to assist clinicians in identifying subtle discomfort and potential sources or to scan for infrequent neurologic or cardiac-rela...
Nielsen SS, Alvarez J, Boklund A, Dippel S, Dorea F, Figuerola J, Herskin M, Miranda Chueca MA, Nannoni E, Nonno R, Riber A, Stahl K, Stegeman JA....The objective of this Scientific Opinion is to assess the hazards and welfare consequences associated with the slaughter of horses for human consumption. The entire slaughter procedure, from arrival at the slaughterhouse until death, is divided into three phases: Phase 1 - pre-stunning, Phase 2 - stunning and Phase 3 - bleeding. Phase 1 includes the following processes (in chronological order): (a) arrival, (b) unloading of the animals from the vehicle, (c) lairage, (d) handling and moving to the stunning area and (e) restraint before application of the stunning method. Phase 2 encompasses the...
Nielsen SS, Alvarez J, Boklund A, Dippel S, Dorea F, Figuerola J, Herskin M, Miranda Chueca MA, Nannoni E, Nonno R, Riber A, Stahl K, Stegeman JA....Horses of different ages may have to be killed on-farm for purposes other than slaughter (where slaughter is defined as killing for human consumption) either individually (i.e. on-farm killing of unproductive, injured or terminally ill animals) or on a large-scale (i.e. depopulation for disease control purposes and other situations, such as environmental contamination, disaster management, etc.). The purpose of this opinion is to assess the hazards and welfare consequences associated with the on-farm killing of horses. The killing procedure is divided into Phase 1 (pre-killing), which includes...
Fiedler JM, Ayre ML, Rosanowski S, Slater JD.Organisations for which sentient animals are central to the business model need to demonstrate the safeguarding of animal welfare and well-being. This requires providing positive experiences for animals which is critical to maintaining the social licence to operate. A cross-sectional survey captured the attitudes of experienced horse sector participants regarding sentience, welfare and well-being. Almost all respondents (99.9%; n = 676/677), believed horses were sentient. Analysis of open-ended responses identified two themes: (1) Sentience is a pathway to understanding the mental state, welfa...
François AC, Cesarini C, Taminiau B, Renaud B, Kruse CJ, Boemer F, van Loon G, Palmers K, Daube G, Wouters CP, Lecoq L, Gustin P, Votion DM.Hypoglycin A and methylenecyclopropylglycine are protoxins responsible for atypical myopathy in equids. These protoxins are converted into toxins that inhibit fatty acid β-oxidation, leading to blood accumulation of acylcarnitines and toxin conjugates, such as methylenecyclopropylacetyl-carnitine. The enzymes involved in this activation are also present in some prokaryotic cells, raising questions about the potential role of intestinal microbiota in the development of intoxication. Differences have been noted between the faecal microbiota of cograzers and atypical myopathy-affected horses. Ho...
Haussler KK, le Jeune SS, MacKechnie-Guire R, Latif SN, Clayton HM.The defining characteristic of laterality is the dominance of one side of the brain controlling specific functions of paired organs or on one side of the body. Structural and functional asymmetries are ubiquitous in horses and range from anatomical features (e.g., the length of long bones) to the gathering of sensory information (e.g., which eye is used to observe unfamiliar scenes) and motor functions (e.g., left-right differences in locomotion). There is a common tendency to assign observed structural or functional asymmetries to lateralization, which often involves more than a simple left-r...
Leishmaniosis is a vector-borne and zoonotic disease with major sanitary implications at global scale. In Europe, Leishmania infantum is the only endemic etiologic agent, which has been previously reported in a wide range of mammal. However, the information about the role of equids in the L. infantum epidemiology is limited. We aimed to assess the seroprevalence and identify potential risk factors associated with L. infantum seropositivity in equine populations across several European countries. A total of 1364 equids, including 1005 horses, 240 donkeys and 119 mules/hinnies from Spain, Italy,...
Du W, Sun Q, Hu S, Yu P, Kan S, Zhang W.Mitochondria are semi-autonomous organelles that play a crucial role in the energy budget of animal cells and are closely related to the locomotor abilities of animals. Equidae is renowned for including two domesticated species with distinct purposes: the endurance-oriented donkey and the power-driven horse, making it an ideal system for studying the relationship between mitochondria and locomotor abilities. In this study, to cover the genetic diversity of donkeys, we sequenced and assembled six new mitochondrial genomes from China. Meanwhile, we downloaded the published mitochondrial genomes ...
Sheahan BJ, Schubert AG, Schubert W, Sheats MK, Schnabel LV, Gilbertie JM.Neutrophil extracellular traps (NETs) play a significant role in response to a variety of infectious and inflammatory stimuli in human and veterinary medicine. Although entrapment of bacteria can be an important function of NETs, the exuberant release of DNA and other intracellular molecules has also been negatively implicated in the pathogenesis of different diseases. Thus, NET formation must be tightly controlled and represents an opportunity for therapeutic interventions. Horses are particularly sensitive to bacterial stimuli that have previously been shown to cause NETs in other species, b...
Verhaar N, Geburek F.Clinical intestinal viability assessment is associated with significant limitations, and there is an undisputable need for ancillary diagnostics during colic surgery. Human and companion animal surgeons struggle with similar intraoperative issues, yet there is little exchange between specialists. Therefore, this narrative review aimed to create an overview of real-time ancillary diagnostics with the potential for intraoperative intestinal viability assessment in horses. Most real-time ancillary diagnostics can be classified as either tissue perfusion or oxygenation assessments. Intestinal perf...
Qiu Y, Jiang J, Yi X, Wang S, Sun X.This study investigated the immunoglobulins (IG) gene locus structure and expression diversity in local Chinese horse and donkey breeds, including Ningqiang, Guanzhong horses, and varieties such as Guanzhong, Jiami, and Northern Shaanxi donkey using genome alignment and high-throughput sequencing. The aim was to expand understanding of IG expression patterns in horses, donkeys, and their different breeds. The results revealed that the donkey IGH locus contains 117 VH genes (23 functional), 44 DH genes, and 8 JH genes, arranged in a VH-DH-JH sequence on chromosome 7, spanning approximately 1189...
Vall E, Blanchard M, Sib O, Cormary B, González-García E.In tropical regions, subject to significant fluctuations in feedstuffs, the body condition score (BCS) is a relevant indicator for monitoring body reserves status of farm animals. However, the most used BCS grids comes from temperate conditions and still not adapted for tropical species, which limits adoption and usefulness. The current work presents for the first time an original, low-cost, standardized BCS assessment system, suited to a large spectrum of tropical farm animals (zebu and/or crossbred cattle, buffaloes, camel, sheep, goats, horses and donkeys). Based on a rigorous set of unifor...
Buono F, Castaldo E, Veneziano V, Veronesi F, Roncoroni C, Scarcelli S, Sgroi G, Matthews JB, Piantedosi D.Grazing horses are exposed to various intestinal helminths, with tapeworms being of major concern due to their potential pathogenicity. Few regional data are available in Italy on tapeworm infections in horses; therefore, the aim of this study was to investigate the prevalence of Anoplocephala spp. eggs over a nine-year period (2014-2023) in 12,056 faecal samples from horses living in all 18 continental Italian regions. Coprological examinations were performed using the Proudman and Edwards (1992) method using Sheather's sugar solution as the floatation medium. An overall prevalence of 3.7 % ...
Science (New York, N.Y.)March 28, 2025
Volume 387, Issue 6741 eadr8589 doi: 10.1126/science.adr8589
Castiglione GM, Chen X, Xu Z, Dbouk NH, Bose AA, Carmona-Berrio D, Chi EE, Zhou L, Boronina TN, Cole RN, Wu S, Liu AD, Liu TD, Lu H, Kalbfleisch T....Horses are among nature's greatest athletes, yet the ancestral molecular adaptations fueling their energy demands are poorly understood. Within a clinically important pathway regulating redox and metabolic homeostasis (NRF2/KEAP1), we discovered an ancient mutation-conserved in all extant equids-that increases mitochondrial respiration while decreasing tissue-damaging oxidative stress. This mutation is a de novo premature opal stop codon in KEAP1 that is translationally recoded into a cysteine through previously unknown mechanisms, producing an R15C mutation in KEAP1 that is more sensitive to ...
Nielsen MK, Bartholdy ID, Kristensen KS, Borrye JC, Meilvang KSS, Rendtorff CEK, Hjortflod MD, Fuglbjerg V, Karlsson M, Petersen HH, Toft K....Equine strongylids are ubiquitous and can cause severe health issues. Anthelmintic resistance is widely common in cyathostomin parasites, and recent studies have documented increasing incidence of resistance to the macrocyclic lactone drug class. Several European countries have implemented prescription-only restrictions of anthelmintic usage by law to reduce anthelmintic treatment intensity and decrease the selection pressure for drug resistance. However, long term outcomes of this approach have not been thoroughly evaluated. The aim was to determine ivermectin efficacy in strongylids by means...
MacKechnie-Guire R, Clayton H, Williams J, Marlin D, Fisher M, Fisher D, Walker V, Murray RC.Information describing forces applied to the horse are needed to inform regulatory decisions regarding equine health and wellbeing. This study compares forces exerted beneath the noseband and headpiece of a snaffle bridle (SB) and a double bridle (DB). Horses were fitted with the same type of SB and DB. Forces were measured by pressure mats under the noseband (nasal/mandibular) and headpiece (occipital) of the bridle and by force sensors inserted bilaterally between the bit(s) and reins. The noseband was adjusted to 2 finger-equivalents using a tightness gauge. Data were recorded for eleven hi...
