Equine health encompasses the study and management of diseases, disorders, and overall well-being of horses. It involves understanding various physiological systems, preventive care, and treatment strategies to maintain optimal health in equine populations. Common areas of focus include nutrition, infectious diseases, orthopedic conditions, and reproductive health. Research in equine health aims to advance knowledge on diagnostic methods, therapeutic interventions, and management practices that improve horse welfare and performance. This page collects peer-reviewed research studies and scholarly articles that explore the diverse aspects of equine health, offering insights into current findings and advancements in the field.
Dersch K, Kreuder A, Wang C, Troy J.Surgical site infections (SSI) are a common postoperative complication of equine orthopedic surgery and often increase morbidity and mortality. Objective: This study aimed to determine if covering surgical instruments during surgery decreased bacterial environmental air contamination during early opening of surgical packs (EOSP). The authors hypothesized that covering instruments with sterile surgical towels would reduce the total number of bacterial colonies to which the instruments were exposed. Methods: Blood agar plates (BAP) were allocated into two groups (non-covered vs. covered with a s...
Epizootic lymphangitis (EEL), caused by Histoplasma capsulatum var. farciminosum (HCF), is a neglected equine fungal disease lacking effective vaccines. The newly developed inactivated '8ZH' vaccine requires safety validation in the target species. Objective: To evaluate the biochemical, haematological and clinical safety of the inactivated HCF '8ZH' vaccine in foals. Methods: A controlled, single-blinded study was conducted on 30 clinically healthy foals (4-6 months), randomized into vaccinated (n = 15) and control (n = 15) groups. Vaccinated animals received a 5 mL intramuscular dose (...
Desta TT.Despite their use in rural and urban transport systems, horses are often abandoned when they are aged and/or face health problems. Abandoned horses typically experience a miserable life, which is ethically unacceptable. This piece of work reports on the suffering of an abandoned cart horse and proposes strategies to improve the welfare of such horses. A type of pension program or provident fund and sanctuary should be established for working animals to lessen their suffering after abandonment. Comprehensive studies should be conducted to gather more data on the suffering of abandoned horses an...
Moroni R, Fanelli D, Maltinti S, Orefice M, Rota A, Camillo F, Melanie P, Cantile C, Miragliotta V, Pirone A, Lazzarini G, Passamonti F, Marmorini P....Equine persistent breeding-induced endometritis can lead to chronic uterine inflammation and fibrosis, reducing fertility. Intrauterine ozone (O₃) has recently been proposed as a potential treatment for mares unresponsive to conventional therapies. This case series describes O₃ treatment in eleven mares barren for at least one year despite multiple inseminations attempts. During the first oestrous cycle, low-volume uterine lavage and endometrial biopsy were performed, followed by 3 consecutive days of intrauterine O₂-O₃ gas insufflation. In the subsequent cycle, both procedures were re...
Goetz M, Cremoux M, Lassalle L, Douet JY.To assess the efficacy and safety of Rose Bengal and green light corneal cross-linking (RGX) as an adjunctive treatment for complicated corneal ulcers and stromal abscesses in horses. Methods: A retrospective analysis included 81 horses (82 eyes) treated with RGX between 2018 and 2024. Cases involved complicated corneal diseases such as melting keratitis, fungal keratitis, deep stromal defects, and stromal abscesses. Treatment consisted of topical or iontophoretic administration of 1% Rose Bengal followed by green light irradiation (550 nm; 150 J/cm) for 10 min. Most procedures (76.8%) w...
D'el Rey Dantas FT, Forni G, Hallowell G, Castagnetti C, Menchetti L, Romito G, Lanci A, Mariella J, Freccero F.Bodyweight, age and breed influence the echocardiographic assessment of foals. There are no echocardiographic studies in Standardbred neonatal foals. Objective: To describe echocardiographic values for selected variables, evaluate intra- and inter-observer variability and assess cardiac changes in the first 5 days of life in healthy Standardbred neonatal foals. Methods: Prospective observational study. Methods: Fifty-six healthy Standardbred neonatal foals were examined by transthoracic echocardiography using standard right parasternal and subcostal views at three time points: in the first 4...
Valente TC, Mendes RP, Miguel EL, Rafael LA, Ribeiro GSN, Souza AF, Fonteque JH.Regional intravenous limb perfusion (RILP) is widely used in equine practice for achieving high local antimicrobial concentrations while minimizing systemic exposure. However, certain drugs, including enrofloxacin, have been associated with vascular irritation and thrombosis, and their thrombogenic potential remains poorly characterized. Objective: This study aimed to evaluate the thrombogenic potential of enrofloxacin administered via RILP in the cephalic vein of clinically healthy horses. Methods: Ten adult horses underwent RILP in both forelimbs: the right limb received 10 % enrofloxacin (1...
Hämäläinen MJ, Brotherus IL, Wigren HM, Kaimio TE, Suomala H, Olbricht AM, Hänninen LT, Mykkänen AK.Reduced rapid-eye-movement (REM) sleep is associated with impaired learning in many species. We developed a reversal learning test (RLT) suitable for field conditions to explore this association in 16 healthy horses. Nocturnal REM-like sleep behavior was recorded five times for 48 h over a six-week period. The horses performed a target training task followed by an RLT using two objects. When the horses reached a predefined frequency of touching the object, the spatial location was reversed. Mean test parameters were statistically analysed using GENLIN models, longitudinal continuous variables...
Khalil WA, Sharf MI, Derbala MK, Alfattah MA, Hassan MAE, Alhujaili W, El-Harairy MA, Abdelnour SA.Oxidative stress is a major contributor to male infertility. Therefore, fortifying assisted reproductive technology with nanotechnology could enhance sperm preservation. Objective: This study aimed to examine the impact of myo-inositol nano-emulsion (MINE) supplementation in semen extender on sperm quality, redox balance, semen bacteriology, apoptosis, ultrastructure, and acrosome status of chilled stallion semen. Methods: Semen samples were collected and preserved with 0 (MINE0), 1 (MINE1), and 2 (MINE2) mg of MINE/mL of extender. Results: Results revealed that extender fortified with 1 or 2 ...
Choi Y, Jung Y, Wickens CL, Yoon M.Horses are social animals, with early life experiences playing a crucial role in their physiological and behavioral development. This study explored the influence of herd dynamics and foundational training on behavioral and hormonal changes in weaned foals. We examined 13 six-month-old Quarter Horse foals over a three-month training period, performing behavioral observations, temperament assessments, and hormone analyses at the start, midpoint, and end of the training. The results indicated that affiliative behaviors decreased significantly, while agonistic behaviors first increased and then d...
Hansen T, Kendall A, Finne R, Law E, Ringdahl A, Nostell K.Abdominal ultrasound is widely used to evaluate the intestinal tract of horses. Despite being a routine examination, there is limited data on the reliability of this diagnostic procedure. Objective: To investigate intra- and inter-rater reliability of ultrasonographic intestinal wall thickness measurements in healthy horses. A second aim was to assess variance within repeated measurements to determine threshold values that distinguish whether differences between repeated examinations are true findings versus solely due to measurement variation. Methods: In vivo reliability study. Methods: Eigh...
Vallejo-Soto P, Dorado J, Herrera-García R, Álvarez-Delgado C, Gómez-Laguna J, Santiago Á, Manrique M, González Ariza A, León Jurado JM....Assisted reproductive techniques are often extrapolated from horses to donkeys, despite poorer fertility outcomes in jennies. This issue has been attributed to unknown uterine species-specific differences. This study compared, through histomorphometry, the endometrium of jennies and mares. Endometrial biopsies (N = 12) were taken from reproductively sound jennies (n = 6) and mares (n = 6) in estrus. Histomorphometric analysis evaluated luminal (LE, µm) and glandular epithelium height (GE, µm), glandular lumen diameter (LD, µm), glandular area (GA, µm), the number of glands (#G), and glandu...
Țăpuc IM, Mureșan AN, Mircean M, Papuc I.Urinalysis is a valuable, non-invasive method for evaluating renal function, yet data on donkey urinary parameters remain scarce. This study characterised urinary sediment in fifty-four healthy adult jennies, established urine specific gravity (USG) reference intervals, and proposed a preliminary semi-quantitative sediment grading scale. Urine samples were collected via catheterisation and analysed using refractometry, dipstick testing, and microscopy. The median USG was 1.048 (IQR: 1.040-1.055) with a pH of 7.6 (IQR: 7.2-8.0). Leukocytes and glucose were absent; erythrocytes were found in one...
Riquelme LF, de Araújo VO, da Silva TKF, de Sousa MS, da Silva MA, da Silva WDQ, Lucena RB.Malachra fasciata Jacq. (Malvaceae) is an invasive plant widely found in Northeastern Brazil and has been experimentally demonstrated to cause severe dermatitis in sheep. However, its toxic effects in horses have not previously been documented. This report describes primary photosensitization in four Quarter Horses grazing on a pasture heavily infested with M. fasciata in Paraíba, Brazil. Affected animals developed alopecic, erythematous, crusted, and ulcerated lesions on sun-exposed areas, accompanied by severe pruritus and behavioral signs of distress. Histopathology revealed epidermal ulce...
Ledeck J, Dubrowski T, Schoumacher M, Peeters S, Le Goff C, Egyptien S, Deleuze S, Cavalier E, Ponthier J.Refined profiling of conjugated estrogens and androgens during equine pregnancy using liquid chromatography-tandem mass spectrometry (LC-MS/MS) could provide accurate fetal-sex determination. Current methods for fetal-sex prediction remain limited by timing, accuracy, and operator expertise. This study investigated sex-specific differences in maternal conjugated steroid profiles to develop a reliable, non-invasive predictive method. Samples were collected from 141 mares of various breeds starting at sixteen weeks of pregnancy. The samples were pooled according to gestational stage, divided int...
McCoy AM, Lopp-Schurter CT, Bishop RC, Narotsky A, Grogger K, Kemper AM.To determine the natural progression of tarsal osteochondrosis (OC) in a cohort of Standardbred foals and assess the impact of gait preference (trotting vs. pacing). Methods: Longitudinal observational cohort study. Methods: Client-owned Standardbred foals (n = 148). Methods: Tarsal radiographs were taken every 2 months from 2 to 12 months of age and foals were video monitored to document time spent pacing or trotting. Differences between groups were assessed using χ analysis. Survival analysis was used to determine if lesion healing differed between groups over time. Results: Of 148 ...
Jones CJP, Aplin JD, Wilsher S.There is little information on non-ciliated secretory cell numbers in the equine oviduct isthmus over the course of the estrous cycle and the effect, if any, of insemination. Lectin histochemistry was used on a series of 13 oviducts taken at different stages of the estrous cycle, with and without insemination, to monitor glycosylation changes and the distribution of secretory cells characterised by a prominent cytoplasmic accumulation of glycoconjugates. There appeared to be an increase in secretory cells expressing a wide range of glycans in all specimens at estrus. One day after ovulation, o...
Klass LG, Krücken J, Mbedi S, Sparmann S, Schenk T, Andreotti S, von Samson-Himmelstjerna G.Mixed strongyle infections represent the most prevalent equine parasitosis and can result in life-threatening disease, especially in young horses. Species involvement and pathogenesis of this parasitosis are poorly understood, and data on foals and broodmares are notably lacking. Methods: In a longitudinal study undertaken in 2022 in Germany, individual faecal samples (n = 497) and metadata were collected for naturally infected foals and broodmares (n = 48) kept under conventional husbandry conditions. Nematode infections were detected coproscopically via the Mini-FLOTAC method. In a s...
Jolley EN, Higgins AH, Logan AA.Asymmetry of stirrups and rider experience level has shown to impact rider symmetry and position. Objective: This study aims to determine if novice and advanced riders can detect stirrup asymmetry in the same frequency, hypothesizing that advanced riders would detect asymmetry in a greater frequency. Methods: Ten stock-type horses and 10 (5 advanced, 5 novice) riders, paired together to test random stirrup treatments. Riders were assigned to a horse for 2 rides, one with asymmetric stirrups, the other with symmetric stirrups, but blinded to their treatments. At the end of both rides, they took...
Cantarelli C, Cribb NC, Delli-Rocili M, Brisson B, Zur Linden A, Caswell JL.Long-term assessment of nephrosplenic space (NSS) closure and comparisons with ultrasonography and rectal palpation are lacking. The objective was to evaluate NSS closure in 12 research horses 5 y following laparoscopic ablation, using a novel adhesion scoring system, and to assess whether rectal examination and ultrasonographic findings could predict adhesion adequacy. The NSS was examined rectal palpation and ultrasonography prior to surgery, and at 30 d and 5 y after surgery. Ultrasonographic measurements of the distances between the renal fascia and spleen were recorded. The NSS adhesions...
Baird JD, Arroyo LG, Lumsden JH.The objective of this study was to determine the effect of either oral supplementation of sodium selenite and alpha-tocopherol or intramuscular administration of a commercial preparation of selenium (Se) and vitamin E to Standardbred broodmares with low (21.0 ± 18.1 IU/g Hb) whole blood glutathione peroxidase (GPx) activity. Ten mares received 3 weekly intramuscular (IM) doses, whereas 9 mares were orally supplemented daily for 10 wk. Blood samples were collected jugular venipuncture and the whole blood GPx activity was measured at each sampling time for the 32-week period. Both oral and int...
Vilela VMT, Zutter BM, Gomes LLO, Silva DF, Diniz JHW, Sancler-Silva YFR, Ignácio FS, Papa FO, Monteiro GA.This study investigated the effects of cholesterol-loaded cyclodextrin (CLC) before cryopreservation and the use of FertTalp (FT) after thawing on the structural and functional quality of equine spermatozoa. Methods: Two ejaculates from twelve stallions were divided into four groups: G1 (0 mg CLC), G2 (1.0 mg), G3 (1.5 mg), and G4 (2.0 mg). Post-thaw semen quality was evaluated through computer-assisted sperm analysis and flow cytometry. Results: In Experiment I, supplementation with 1.5 mg (G3) significantly improved total motility (64.9 ± 10.1 %) and progressive motility (40.5 ± 8.8 %) com...
Puccetti M, Beccati F, Pilati N.Femoral nerve neuropathy causes lameness, a condition clinically characterized by stifle's lack of extension with subsequent diminished lower limb function. This case report describes the ultrasonographic diagnosis of femoral neuropathy secondary to compression caused by myopathy of the iliopsoas muscle of an endurance horse during a competition. Transrectal ultrasonography played a pivotal role in diagnosis of the injury by assessment of the involved structures and monitoring of the healing process throughout the recovery period. The horse achieved complete recovery and successfully returned ...
Gmel AI, Haraldsdóttir EH, Rosa TV, Lamas LP, Neuditschko M, Weishaupt MA.Europe has many horse breeds differing in conformation, gaits and genetics due to diverging breeding goals. Research studies comparing objective gait parameters between different horse breeds are limited and date back a decade. Objective: In this study, objective gait parameters were measured in three European breeds, namely Lusitanos (LUS; n = 52), Franches-Montagnes (FM; n = 311) and Swiss Warmblood (WB; n = 40) using an inertial measurement system (sensors at poll, withers, pelvis and all four canon bones) at walk and trot. Results: At walk and trot, LUS moved significantly slower than WB a...
Sayre KC, Flores-Ahlschwede P, Wethington AB, Kahn SK, Bray JM, Volding DM, Ahlschwede S, Bordin AI, Cohen ND, Welsh TH.Transfusion of Rhodococcus equi hyperimmune plasma (REHIP) to neonatal foals is done to reduce the incidence of rhodococcal pneumonia. Objective: To determine effects in newborn foals of REHIP transfusion on serum cortisol concentrations; the association of serum cortisol concentrations with serum globulin concentration and anti-rhodococcal immunoglobulin activity; and cumulative incidence of rhodococcal pneumonia. Methods: Serum samples were collected from 356 newborn foals from 13 New York farms immediately before and after transfusion of 1 L or 2 L of REHIP. Serum samples were assayed for c...
Key K, Berg K, Kirkegaard J, Andresen KR, Hansen SS.Equine lameness diagnosis largely relies on subjective visual assessments, which can be biased. Although marker-based methods, force plates and inertial measurement units (IMUs) provide objective measurements, they require specialized setups. Vision-based algorithms offer a portable, markerless alternative, but their accuracy needs thorough testing. Objective: To evaluate a custom vision-based algorithm for estimating the groundline across multiple camera angles, including handheld use in horses trotting on a treadmill. Methods: Experimental comparative study. Methods: Eight Standardbred trott...
Hansen S, Tecles F, Cerón JJ, From C, Carstensen H, Muñoz-Prieto A.The number of horses with equine squamous gastric disease (ESGD) and equine gastric glandular disease (EGGD) recurrence when pharmacological treatment is discontinued is high. Objective: To examine if a commercially available nutraceutical compound containing lecithin, pectin, and meadowsweet could prevent recurrence of both ESGD and EGGD after omeprazole treatment, evaluated by repeated gastroscopic examinations and saliva biomarkers. Methods: Blinded, randomised, placebo-controlled clinical trial. Methods: Thirty horses of mixed breeds and sex with a recent diagnosis and treatment of both ES...
Amaje J, Upton S, Garba UM, Jolayemi KO.Hay nets and slow feeders are designed to mimic natural grazing by prolonging forage intake, thereby supporting digestive health and reducing stress-related behaviours. Their design and use, however, can influence effectiveness and may introduce risks. Objective: This semi-systematic review evaluated the use of hay nets and slow feeders in feeding horses, focusing on their impact on feeding time, behaviour, forage management and health management. Methods: A semi-systematic search across PubMed, ScienceDirect, Google Scholar, and Scopus identified 26 full-text studies published between 2009 an...
Singer J, Chow L, Ammons D, Sabino I, Impastato R, Dow S, Pezzanite LM.Osteoarthritis (OA) is a aging-associated degenerative joint disease. The objective was to determine relative senescence gene expression in joints and leukocytes of OA horses toward considering senotherapeutics to manage OA. Unassigned: To define local (joint) and systemic (peripheral blood mononuclear cells [PBMCs]) senescence burden, synovial fluid cell single-cell RNA sequencing and PBMC mRNA sequencing datasets (n = 65 samples) were examined. Differential analyses were conducted using limma to compare OA versus control. A custom 3,043-gene senescence set curated from published metadata was...
Nixon AJ, Dahlgren LA, Haupt JL, Yeager AE, Ward DL.To assess the potential of adipose-derived nucleated cell (ADNC) fractions to improve tendon repair in horses with collagenase-induced tendinitis. Methods: 8 horses. Methods: Collagenase was used to induce tendinitis in the superficial digital flexor tendon of 1 forelimb in each horse. Four horses were treated by injection of autogenous ADNC fractions, and 4 control horses were injected with PBS solution. Healing was compared by weekly ultrasonographic evaluation. Horses were euthanatized at 6 weeks. Gross and histologic evaluation of tendon structure, fiber alignment, and collagen typing were...
Frisbie DD, Al-Sobayil F, Billinghurst RC, Kawcak CE, McIlwraith CW.To discriminate between changes in biomarkers with exercise compared to changes in biomarkers with osteoarthritis (OA) in exercising horses. Methods: Sixteen, 2-year-old horses were randomly assigned either to an exercise-alone (n=8) or OA-affected (also exercised) (n=8) group. All horses had both mid-carpal joints arthroscoped and OA induced in one mid-carpal joint in the OA-affected joints of OA-affected horses. Two weeks after surgery all horses commenced a strenuous exercise program on a high-speed treadmill. Clinical outcomes and synovial fluid and serum biomarkers, were evaluated weekly....
Voga M, Adamic N, Vengust M, Majdic G.Regenerative medicine is a branch of medicine that develops methods to grow, repair, or replace damaged or diseased cells, organs or tissues. It has gained significant momentum in recent years. Stem cells are undifferentiated cells with the capability to self-renew and differentiate into tissue cells with specialized functions. Stem cell therapies are therefore used to overcome the body's inability to regenerate damaged tissues and metabolic processes after acute or chronic insult. The concept of stem cell therapy was first introduced in 1991 by Caplan, who proposed that massive differentiatio...
Hondalus MK, Mosser DM.Rhodococcus equi is a facultative intracellular bacterium of macrophages that can cause serious pneumonia in both young horses and immunocompromised people. Essential to understanding rhodococcus pathogenesis is a quantitative documentation of the intracellular events that follow macrophage phagocytosis of the organism. By using a bacterial immunofluorescence staining assay, we verified the intracellular survival and replicative potential of R. equi in both murine peritoneal macrophages and equine alveolar macrophages in vitro. Following an initial lag period of 6 to 12 h, the intracellular nu...
Gaspar D, Spanoudes K, Holladay C, Pandit A, Zeugolis D.The last decade has seen significant developments in cell therapies, based on permanently differentiated, reprogrammed or engineered stem cells, for tendon injuries and degenerative conditions. In vitro studies assess the influence of biophysical, biochemical and biological signals on tenogenic phenotype maintenance and/or differentiation towards tenogenic lineage. However, the ideal culture environment has yet to be identified due to the lack of standardised experimental setup and readout system. Bone marrow mesenchymal stem cells and tenocytes/dermal fibroblasts appear to be the cell populat...
Hauswirth R, Haase B, Blatter M, Brooks SA, Burger D, Drögemüller C, Gerber V, Henke D, Janda J, Jude R, Magdesian KG, Matthews JM, Poncet PA....During fetal development neural-crest-derived melanoblasts migrate across the entire body surface and differentiate into melanocytes, the pigment-producing cells. Alterations in this precisely regulated process can lead to white spotting patterns. White spotting patterns in horses are a complex trait with a large phenotypic variance ranging from minimal white markings up to completely white horses. The "splashed white" pattern is primarily characterized by an extremely large blaze, often accompanied by extended white markings at the distal limbs and blue eyes. Some, but not all, splashed white...
Keegan KG, Dent EV, Wilson DA, Janicek J, Kramer J, Lacarrubba A, Walsh DM, Cassells MW, Esther TM, Schiltz P, Frees KE, Wilhite CL, Clark JM....Previous studies have suggested that agreement between equine veterinarians subjectively evaluating lameness in horses is low. These studies were limited to small numbers of horses, evaluating movement on the treadmill or to evaluating previously-recorded videotape. Objective: To estimate agreement between equine practitioners performing lameness evaluations in horses in the live, over ground setting. Methods: 131 mature horses were evaluated for lameness by 2-5 clinicians (mean 3.2) with a weighted-average of 18.7 years of experience. Clinicians graded each limb using the AAEP lameness scale ...
Schneider J, Kaaden O, Copeland TD, Oroszlan S, Hunsmann G.Two glycopolypeptides with molecular weights 160,000 and 120,000 (gp120) are regularly recognized by human immunodeficiency virus (HIV)-specific antisera in lysates of cells persistently infected with HIV. In the present study, gp120 was characterized as the major envelope glycopolypeptide of HIV. Gp120 was identified as the external viral glycoprotein by radiosequencing and by its presence in purified virus. However gp120 was predominantly shed as a soluble protein into the culture fluid. Furthermore gp120 was precipitated by sera from horses infected with equine infectious anaemia virus (EIA...
Tetens J, Widmann P, Kühn C, Thaller G.A genome-wide association scan for loci affecting withers height was conducted in 782 German Warmblood stallions, which were genotyped using the Illumina EquineSNP50 Bead Chip. A principal components approach was applied to correct for population structure. The analysis revealed a single major QTL on ECA3 explaining ~18 per cent of the phenotypic variance, which is in concordance with recent reports from other horse populations. The LCORL/NCAPG locus represents a strong candidate gene for this QTL. This locus is among a small number that have consistently been identified to influence human hei...
Rivas F, Diaz LA, Cardenas VM, Daza E, Bruzon L, Alcala A, De la Hoz O, Caceres FM, Aristizabal G, Martinez JW, Revelo D, De la Hoz F, Boshell J....In 1995, the first Venezuelan equine encephalitis (VEE) outbreak in Colombia in 22 years caused an estimated 75,000 human cases, 3000 with neurologic complications and 300 fatal, in La Guajira State. Of the state's estimated 50,000 equines, 8% may have died. An epizootic IC virus, probably introduced from Venezuela, was rapidly amplified among unvaccinated equines. Record high rainfall, producing high densities of vector Aedes taeniorhynchus, led to extensive epidemic transmission (30% attack rate) in the four affected municipalities. Native Wayuu Indians, constituting 24% of the state's popul...
Bosch G, van Schie HT, de Groot MW, Cadby JA, van de Lest CH, Barneveld A, van Weeren PR.Tendon injuries are notorious for their slow and functionally inferior healing. Intratendinous application of platelet-rich plasma (PRP) has been reported to stimulate the repair process of tendon injuries, but there is little conclusive evidence for its effectiveness. A placebo-controlled experimental trial was performed to test the hypothesis that a single intratendinous PRP treatment enhances the quality of tendon repair, as evidenced by improved biochemical, biomechanical, and histological tissue properties. In six horses, tendon lesions were created surgically in the Superficial Digital F...
Hillier ML, Bell LS.This review brings together a complex and extensive literature to address the question of whether it is possible to distinguish human from nonhuman bone using the histological appearance of cortical bone. The mammalian species included are rat, hare, badger, racoon dog, cat, dog, pig, cow, goat, sheep, deer, horse, water buffalo, bear, nonhuman primates, and human and are therefore not exhaustive, but cover those mammals that may contribute to a North American or Eurasian forensic assemblage. The review has demonstrated that differentiation of human from certain nonhuman species is possible, i...
Fureix C, Jego P, Henry S, Lansade L, Hausberger M.Recent reviews question current animal models of depression and emphasise the need for ethological models of mood disorders based on animals living under natural conditions. Domestic horses encounter chronic stress, including potential stress at work, which can induce behavioural disorders (e.g. "apathy"). Our pioneering study evaluated the potential of domestic horses in their usual environment to become an ethological model of depression by testing this models' face validity (i.e. behavioural similarity with descriptions of human depressive states). Results: We observed the spontaneous behav...
Russo AT, White MA, Watowich SJ.Alphavirus replication and propagation is dependent on the protease activity of the viral nsP2 protein, which cleaves the nsP1234 polyprotein replication complex into functional components. Thus, nsP2 is an attractive target for drug discovery efforts to combat highly pathogenic alphaviruses. Unfortunately, antiviral development has been hampered by a lack of structural information for the nsP2 protease. Here, we report the crystal structure of the nsP2 protease (nsP2pro) from Venezuelan equine encephalitis alphavirus determined at 2.45 A resolution. The protease structure consists of two dist...
Tulman ER, Delhon G, Afonso CL, Lu Z, Zsak L, Sandybaev NT, Kerembekova UZ, Zaitsev VL, Kutish GF, Rock DL.Here we present the genomic sequence of horsepox virus (HSPV) isolate MNR-76, an orthopoxvirus (OPV) isolated in 1976 from diseased Mongolian horses. The 212-kbp genome contained 7.5-kbp inverted terminal repeats and lacked extensive terminal tandem repetition. HSPV contained 236 open reading frames (ORFs) with similarity to those in other OPVs, with those in the central 100-kbp region most conserved relative to other OPVs. Phylogenetic analysis of the conserved region indicated that HSPV is closely related to sequenced isolates of vaccinia virus (VACV) and rabbitpox virus, clearly grouping to...
Field H, de Jong C, Melville D, Smith C, Smith I, Broos A, Kung YH, McLaughlin A, Zeddeman A.Hendra virus is a recently emerged zoonotic agent in Australia. Since first described in 1994, the virus has spilled from its wildlife reservoir (pteropid fruit bats, or 'flying foxes') on multiple occasions causing equine and human fatalities. We undertook a three-year longitudinal study to detect virus in the urine of free-living flying foxes (a putative route of excretion) to investigate Hendra virus infection dynamics. Pooled urine samples collected off plastic sheets placed beneath roosting flying foxes were screened for Hendra virus genome by quantitative RT-PCR, using a set of primers a...
Wu DD, Yang CP, Wang MS, Dong KZ, Yan DW, Hao ZQ, Fan SQ, Chu SZ, Shen QS, Jiang LP, Li Y, Zeng L, Liu HQ, Xie HB, Ma YF, Kong XY, Yang SL, Dong XX....Abundant and diverse domestic mammals living on the Tibetan Plateau provide useful materials for investigating adaptive evolution and genetic convergence. Here, we used 327 genomes from horses, sheep, goats, cattle, pigs and dogs living at both high and low altitudes, including 73 genomes generated for this study, to disentangle the genetic mechanisms underlying local adaptation of domestic mammals. Although molecular convergence is comparatively rare at the DNA sequence level, we found convergent signature of positive selection at the gene level, particularly the gene in these Tibetan domest...
Metzger J, Karwath M, Tonda R, Beltran S, Águeda L, Gut M, Gut IG, Distl O.Modern horses represent heterogeneous populations specifically selected for appearance and performance. Genomic regions under high selective pressure show characteristic runs of homozygosity (ROH) which represent a low genetic diversity. This study aims at detecting the number and functional distribution of ROHs in different horse populations using next generation sequencing data. Methods: Next generation sequencing was performed for two Sorraia, one Dülmen Horse, one Arabian, one Saxon-Thuringian Heavy Warmblood, one Thoroughbred and four Hanoverian. After quality control reads were mapped t...
Dougal K, Harris PA, Edwards A, Pachebat JA, Blackmore TM, Worgan HJ, Newbold CJ.The microbiome and associated metabolome of faecal samples were compared to those from the caecum and right dorsal colon of horses and ponies euthanised for nonresearch purposes by investigating the microbial population community structure as well as their functional metabolic products. Through the use of 16S rRNA gene dendrograms, the caecum microbiome was shown to cluster separately from the other gut regions. 16S rRNA gene-based quantitative PCR (q-PCR) also demonstrated differences between the caecum and the other gut regions. Metabolites as identified by Fourier transform infrared cluster...
Bussières G, Jacques C, Lainay O, Beauchamp G, Leblond A, Cadoré JL, Desmaizières LM, Cuvelliez SG, Troncy E.This study addresses development and validation of a composite multifactorial pain scale (CPS) in an experimental equine model of acute orthopaedic pain. Eighteen horses were allocated to control (sedation with/without epidural analgesia - mixture of morphine, ropivacaine, detomidine and ketamine) and experimental groups: amphotericin-B injection in the tarsocrural joint induced pain and analgesia was either i.v. phenylbutazone administered post-induction of synovitis, or pre-emptive epidural mixture, or a pre-emptive combination of the 2. Inter- and intra-observer reproducibility was good (0....
Payne RC, Hutchinson JR, Robilliard JJ, Smith NC, Wilson AM.We provide quantitative anatomical data on the muscle-tendon units of the equine pelvic limb. Specifically, we recorded muscle mass, fascicle length, pennation angle, tendon mass and tendon rest length. Physiological cross sectional area was then determined and maximum isometric force estimated. There was proximal-to-distal reduction in muscle volume and fascicle length. Proximal limb tendons were few and, where present, were relatively short. By contrast, distal limb tendons were numerous and long in comparison to mean muscle fascicle length, increasing potential for elastic energy storage. W...
Harman RM, Yang S, He MK, Van de Walle GR.The prevalence of chronic skin wounds in humans is high, and treatment is often complicated by the presence of pathogenic bacteria. Therefore, safe and innovative treatments to reduce the bacterial load in cutaneous wounds are needed. Mesenchymal stromal cells (MSC) are known to provide paracrine signals that act on resident skin cells to promote wound healing, but their potential antibacterial activities are not well described. The present study was designed to examine the antibacterial properties of MSC from horses, as this animal model offers a readily translatable model for MSC therapies i...
Broder CC, Xu K, Nikolov DB, Zhu Z, Dimitrov DS, Middleton D, Pallister J, Geisbert TW, Bossart KN, Wang LF.Hendra virus and Nipah virus are bat-borne paramyxoviruses that are the prototypic members of the genus Henipavirus. The henipaviruses emerged in the 1990s, spilling over from their natural bat hosts and causing serious disease outbreaks in humans and livestock. Hendra virus emerged in Australia and since 1994 there have been 7 human infections with 4 case fatalities. Nipah virus first appeared in Malaysia and subsequent outbreaks have occurred in Bangladesh and India. In total, there have been an estimated 582 human cases of Nipah virus and of these, 54% were fatal. Their broad species tropis...
Hill EW, McGivney BA, Gu J, Whiston R, Machugh DE.Thoroughbred horses have been selected for traits contributing to speed and stamina for centuries. It is widely recognized that inherited variation in physical and physiological characteristics is responsible for variation in individual aptitude for race distance, and that muscle phenotypes in particular are important. Results: A genome-wide SNP-association study for optimum racing distance was performed using the EquineSNP50 Bead Chip genotyping array in a cohort of n = 118 elite Thoroughbred racehorses divergent for race distance aptitude. In a cohort-based association test we evaluated geno...
Walsh C, Gangloff M, Monie T, Smyth T, Wei B, McKinley TJ, Maskell D, Gay N, Bryant C.LPS signals through a membrane bound-complex of the lipid binding protein MD-2 and the receptor TLR4. In this study we identify discrete regions in both MD-2 and TLR4 that are required for signaling by lipid IVa, an LPS derivative that is an agonist in horse but an antagonist in humans. We show that changes in the electrostatic surface potential of both MD-2 and TLR4 are required in order that lipid IVa can induce signaling. In MD-2, replacing horse residues 57-66 and 82-89 with the equivalent human residues confers a level of constitutive activity on horse MD-2, suggesting that conformational...
Ferris DJ, Frisbie DD, Kisiday JD, McIlwraith CW, Hague BA, Major MD, Schneider RK, Zubrod CJ, Kawcak CE, Goodrich LR.To report outcome of horses with femorotibial lesions (meniscal, cartilage or ligamentous) treated with surgery and intra-articular administration of autologous bone marrow derived mesenchymal stem cells (BMSCs). Methods: Prospective case series. Methods: Horses (n = 33). Methods: Inclusion criteria included horses that had lameness localized to the stifle by diagnostic anesthesia, exploratory stifle arthroscopy and subsequent intra-articular administration of autologous BMSCs. Case details and follow-up were gathered from medical records, owner, trainer or veterinarian. Outcome was defined as...
Violini S, Ramelli P, Pisani LF, Gorni C, Mariani P.Mesenchymal stem cells (MSCs) have been recently investigated for their potential use in regenerative medicine. MSCs, in particular, have great potential, as in various reports they have shown pluripotency for differentiating into many different cell types. However, the ability of MSCs to differentiate into tendon cells in vitro has not been fully investigated. Results: In this study, we show that equine bone marrow mesenchymal stem cells (BM-MSCs), defined by their expression of markers such as Oct4, Sox-2 and Nanog, have the capability to differentiate in tenocytes. These differentiated cell...
Carrera JP, Forrester N, Wang E, Vittor AY, Haddow AD, López-Vergès S, Abadía I, Castaño E, Sosa N, Báez C, Estripeaut D, Díaz Y, Beltrán D....The eastern equine encephalitis (EEE) and Venezuelan equine encephalitis (VEE) viruses are pathogens that infect humans and horses in the Americas. Outbreaks of neurologic disease in humans and horses were reported in Panama from May through early August 2010. Methods: We performed antibody assays and tests to detect viral RNA and isolate the viruses in serum samples from hospitalized patients. Additional cases were identified with enhanced surveillance. Results: A total of 19 patients were hospitalized for encephalitis. Among them, 7 had confirmed EEE, 3 had VEE, and 1 was infected with both ...
Olson JG, Ksiazek TG, Gubler DJ, Lubis SI, Simanjuntak G, Lee VH, Nalim S, Juslis K, See R.Sera were collected from humans, cattle, horses, goats, ducks, chickens, wild birds, bats and rats in Lombok, Indonesia, and were tested by haemagglutination inhibition (HI) for antibodies to JE, ZIKA, CHIK and RR. Selected sera were tested by microneutralization tests for antibodies to the following viruses: JE, ZIKA, MVE, TMU, LGT, KUN, SEP, DEN-2, CHIK, RR, GET, SIN, BUN, BAT and BAK. Human sera had JE HI antibody in 135 (30%) of 446 tested. Neutralization tests indicated that DEN-2, ZIKA, TMU, KUN and SEP may have caused flavivirus infections. Antibodies to other arboviruses tested for wer...
