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Topic:Equine Science
Equine Science encompasses the study of horses and their management, health, and performance. This field integrates various scientific disciplines such as biology, genetics, nutrition, physiology, and veterinary medicine to understand and improve the well-being and capabilities of horses. Areas of focus include equine anatomy, reproduction, behavior, and disease prevention. Research in equine science aims to enhance horse care, optimize training and performance, and address health challenges. This page compiles peer-reviewed research studies and scholarly articles that explore the diverse aspects of equine science, providing insights into the latest advancements and methodologies in the field.
Comparative efficacy of two-dimensional mode and color Doppler sonography in predicting gender of the equine fetus. Ultrasonographic fetal sexing is of utmost economic importance for horse breeders. Relatively, a few studies have been conducted to determine fetal sex in mare using transrectal Doppler ultrasound. This study aimed to compare two sexing techniques, two-dimensional (2D) mode and color Doppler ultrasonography. Methods: The study was conducted on 39 mares under field conditions. Examinations were performed using the ultrasonic model device (Medison SonoAce Pico, South Korea), equipped with real-time 3-7 MHz convex multifrequency transducer. Fetal sex diagnosis was carried out in two periods of pr...
Influence of mechanical fluid shear stress on the osteogenic differentiation protocols for Equine adipose tissue-derived mesenchymal stem cells. Cell-based therapies have become a promising approach to promote tissue regeneration and the treatment of musculoskeletal disorders. Bone regeneration maintains bone homeostasis, mechanical stability and physical performance. Mechanical stimulation showed to induce stem cell differentiation into the osteogenic fate. However, the effect of various osteogenic protocols on the osteogenic commitment of equine adipose-derived stem cells is not fully elucidated. Here we examined the influence of fluid-based shear stress (FSS) via mechanical rocking to assess whether mechanical stimulation promotes o...
The application of Lempel-Ziv and Titchener complexity analysis for equine telemetric electrocardiographic recordings. The analysis of equine electrocardiographic (ECG) recordings is complicated by the absence of agreed abnormality classification criteria. We explore the applicability of several complexity analysis methods for characterization of non-linear aspects of electrocardiographic recordings. We here show that complexity estimates provided by Lempel-Ziv '76, Titchener's T-complexity and Lempel-Ziv '78 analysis of ECG recordings of healthy Thoroughbred horses are highly dependent on the duration of analysed ECG fragments and the heart rate. The results provide a methodological basis and a feasible refer...
The Influence of Body Mass and Height on Equine Hoof Conformation and Symmetry. Despite the likelihood that a horse's mass influences hoof morphology, empirical evidence is lacking. A clearer understanding of factors influencing hoof shape could enable prevention, or better treatment, of foot-based disorders; common causes of equine lameness. The study's aim was to investigate the relationship between horse body size, in terms of mass and height, and fore hoof dimensions. A further aim was to determine changes in the occurrence of hoof asymmetry as body size increases. Height, mass, and fore hoof dimensions; coronet band width (CBW); hoof base width (HBW); dorsal hoof wal...
Synergy between MC1R and ASIP for coat color in horses (Equus caballus)1. Through domestication and human selection, horses have acquired various coat colors, including seven phenotypes: black, brown, dark bay, bay, chestnut, white, and gray. Here we determined the genotypes for melanocortin-1 receptor (MC1R) and agouti signaling protein (ASIP) in 709 horses from 15 breeds. We found that the EEEE genotype frequency at MC1R decreased from dark to light colors (black = 64.5%, brown = 67.5%, dark bay = 47.0%, bay = 16.5%, and chestnut = 0.0%), whereas the AAAA genotype frequency at ASIP increased as coat color lightened (black = 0.0%, brown = 22.9%, dark bay = 69.2%, a...
Dynamic Change of Serum Levels of Some Branched-Chain Amino Acids and Tryptophan in Athletic Horses After Different Physical Exercises. Physical exercise induces several adaptations involving modulation of the energetic pathways to meet the energy demand during physical exercise. The branched-chain amino acids, leucine in particular, are considered as the most relevant amino acids, especially for exercise physiology. In this study, the change of leucine, valine, and tryptophan concentration was investigated in athletic horses before and after different exercise type. A total of 40 sport horses were equally divided into 4 groups according to the exercise type: group A (jumping), group B (reining), group C (32 km ride), and grou...
Effect of Mare Colostrum in Extenders for Freezing Stallion Semen. This study aimed to evaluate the addition of mare colostrum in stallion freezing extenders to improve sperm quality. First, colostrum samples were collected from four mares after the foal's birth and their composition was determined. Ejaculates were collected from nine fertile stallions. Sperm samples were pooled, diluted, and cryopreserved into three experimental extender groups: Lactose-based extender supplemented with mare colostrum (20%), lactose-based extender supplemented with egg yolk (20%), and BotuCrio. The quality of the post-thaw semen samples were evaluated assessing sperm motility...
Prevalence and analysis of equine periodontal disease, diastemata and peripheral caries in a first-opinion horse population in the UK. Equine dental disease remains a widespread affliction in domestic horses, with the potential to cause significant welfare problems. This retrospective study aimed to increase understanding of this aspect of equine health by examining dental records of 932 horses, taken from a 1-year period of first-opinion practice in south-western England and southern Wales. Three grossly-identifiable dental diseases were analysed: equine periodontal disease, diastemata and peripheral caries. The prevalence of active periodontal disease, diastemata and peripheral caries were 13.9%, 8.7% and 8.2%, respectively...
Comparative population genomics unveils candidate genes for athletic performance in Hanoverians. Equine athletes have a genetic heritage that has been evolved for millions of years, which provides an opportunity to study the genetics of locomotion pattern and performance in mammals. The Hanoverian, a breed originating in Germany, is arguably among the most athletic of horse breeds, as well as possessing a balanced character and beautiful appearance. Here, we compared the whole genomes of Hanoverian with three other horse breeds (Akhal-Teke, Franches-Montagnes, and Standardbred), using the fixation index (Fst) and cross-population composite likelihood ratio (XP-CLR) methods for testing the...
Detection of ESAs in equine urine and blood by SAR-PAGE. Erythropoiesis-stimulating agents (ESAs) have been used in horses for doping purposes to increase the performance of these animals in endurance sports. Currently, enzyme-linked immunosorbent assay (ELISA) and mass spectrometry methods are used to detect ESA abuse in equines. However, the sarcosyl polyacrylamide gel-electrophoresis (SAR-PAGE) technique could also be used, since its application in human doping control is well established and has proven to be more sensitive. In this work, the SAR-PAGE method was used to detect recombinant human erythropoietin (rHuEPO), novel erythropoiesis stimul...
Subtourniquet pressures generated by application of wide-rubber tourniquets in standing, sedated horses. To determine the influence of location and movement on subtourniquet pressure (STP) generated by application of a wide-rubber tourniquet (WRT) on equine limbs. Methods: Randomized experimental cross-over design. Methods: Six standing, sedated horses. Methods: Horses were sedated with detomidine hydrochloride (0.2 μg/kg IV), and 4 investigators applied WRTs to the antebrachium (AB), gaskin (GK), and midmetacarpus (MC) of each horse in a predetermined, randomized order. Subtourniquet pressure was consequently measured at 10-minute intervals (T0, T10, T20, T30) for 30 minutes. Indirect systo...
Evaluation of equine corneal disease using spectral domain optical coherence tomography (SD-OCT). The purpose of this case series is to evaluate the use of spectral domain optical coherence tomography (SD-OCT) imaging in equine corneal disease to demonstrate the feasibility, diagnostic effectiveness, and limitations of this imaging modality. Methods: Retrospective study: Handheld SD-OCT (Envisu, Bioptigen Inc, Morrisville, NC) was used for high-resolution imaging of horses with corneal disease presenting to the North Carolina State University Equine Ophthalmology Service from 2013 to 2018. Results: Fourteen horses were included in this study, including six horses with corneal ulceration, t...
Morphological and immunohistochemical characteristics of the equine corneal epithelium. The morphology of the corneal epithelium in two age groups of horses is described. Distribution patterns of proliferation-, differentiation-, stem cell-associated markers and cell junction proteins were assessed. Methods: Corneal samples from 12 horses (six foals and six adult horses) were analyzed after H&E staining and immunohistochemistry using the following antibodies: E-cadherin, β-catenin, Connexin 43 (Cx43), tight junction protein 1 (TJP1), cytokeratin (CK) 14, CK 19, CK 3, CK 10, vimentin, Ki67, p63, nerve growth factor (NGF), ABCG2, and epithelial growth factor receptor. Semiquan...
Assessment of two methods to determine the relative contributions of the aerobic and anaerobic energy systems in racehorses. A prospective, randomized, controlled study was designed to determine relative aerobic and anaerobic (lactic and alactic) contributions at supramaximal exercise intensities using two different methods. Thoroughbred racehorses ( = 5) performed a maximal rate of oxygen consumption (V̇o) test and three supramaximal treadmill runs (105, 115, and 125% V̇o). Blood lactate concentration (BL) was measured at rest, every 15 s during runs, and 2, 5, 10, 20, 30, 40, 50, and 60 min postexercise. In , oxygen demand was calculated for each supramaximal intensity based on the V̇o test, and relative aerobi...
Interobserver Reliability of the Animal Welfare Indicators Welfare Assessment Protocol for Horses. Objective tools for the assessment of animal welfare are needed. The present study analyzed the interobserver reliability of the Animal Welfare Indicators (AWIN) welfare assessment protocol for horses to further enhance knowledge concerning reliability. Therefore, two trained observers conducted 18 assessments on farm at the same time and on the same animals. The results were compared at individual level by calculation of Cohen's kappa (κ), weighted kappa (κ), and prevalence-adjusted, bias-adjusted kappa (PABAK). Spearman rank correlation coefficient (RS), intraclass correlation coefficient ...
Feeding Behavior of Mangalarga Marchador Weanlings Fed Sorghum Silage Versus Grass Hay. Mangalarga Marchador foals in Minas Gerais, Brazil, are usually weaned during the dry season, when there is a scarcity of forage. Although sorghum silage has been used to feed weanlings, the effect on their feeding behavior in comparison with grass hay has not been reported. Twelve Mangalarga Marchador weanlings were randomly assigned to two groups of six animals: SS: sorghum silage; VH: Vaquero hay. Both groups received commercial concentrate in the proportion of 1.2 kg.100 kg of the live weight and forage ad libitum. The animals were filmed for 48 hours, being timed: forage intake time, wate...
Effect of dietary supplementation with long-chain n-3 fatty acids during late gestation and early lactation on mare and foal plasma fatty acid composition, milk fatty acid composition, and mare reproductive variables. The effects of dietary marine-derived n-3 fatty acids (FA) on mare milk and mare and foal plasma FA, postpartum ovarian follicular growth and prostaglandin concentrations were evaluated. Sixty days prior to expected foaling dates, mares were assigned to one of three diets: corn oil (CORN, n = 6), docosahexaenoic acid (DHA) diet (D; 12.64 g/d, n = 7), or eicosapentaenoic acid (EPA) and DHA (ED; 8.84 g EPA, 10.43 g DHA/d, n = 7). Milk and plasma were collected for FA analysis. Follicular data were recorded through the first postpartum ovulation. Post-ovulation serial blood samp...
Equine Fecal Microbiota Changes Associated With Anthelmintic Administration. The gastrointestinal microbiota (GIM) plays an essential role in maintaining intestinal homeostasis with disruptions having profound effects on the wellbeing of the host animal. Parasitic infection is a long-standing issue for the equine industry, and the use of anthelmintic drugs for parasite control has long been standard practice. The impact of anthelmintic treatment on the GIM in healthy horses is not well known. This study evaluated the hypothesis that anthelmintic administration will alter the equine fecal microbiota in horses without an observed helminth infection. Ten horses were treat...
Variation in gait parameters used for objective lameness assessment in sound horses at the trot on the straight line and the lunge. Objective lameness assessment is gaining more importance in a clinical setting, necessitating availability of reference values. Objective: To investigate the between -path, -trial and -day variation, between and within horses, in the locomotion symmetry of horses in regular use that are perceived sound. Methods: Observational study with replicated measurement sessions. Methods: Twelve owner-sound horses were trotted on the straight line and on the lunge. Kinematic data were collected from these horses using 3D optical motion capture. Examinations were repeated on 12 occasions over the study wh...
Farm Animal Cognition-Linking Behavior, Welfare and Ethics. Farm animal welfare is a major concern for society and food production. To more accurately evaluate animal farming in general and to avoid exposing farm animals to poor welfare situations, it is necessary to understand not only their behavioral but also their cognitive needs and capacities. Thus, general knowledge of how farm animals perceive and interact with their environment is of major importance for a range of stakeholders, from citizens to politicians to cognitive ethologists to philosophers. This review aims to outline the current state of farm animal cognition research and focuses on u...
Genomic structural diversity in Criollo Argentino horses: Analysis of copy number variations. Copy number variation (CNV) has been proved to be widespread in human, animal and plant genomes. Together with single nucleotide polymorphisms (SNPs), CVNs play a key role in genetic diversity. In this study, genome-wide detection of CNVs was performed based on SNP data from 24 Criollo Argentino horses genotyped with the GGP Equine70k array. Overall, 165 CNVs meeting stringent quality control criteria were identified and then aggregated into 87 CNV regions (CNVRs), representing a horse genome coverage of 13.69 Mb. Functional analysis of CNVRs allowed the identification of 337 genes implicate...
Investigation of the Reliability of Saddle Fitters to Determine the Position of the Last Thoracic Vertebra of Horses Using Palpation Techniques: A Pilot Study. Published guidelines indicate an English saddle tree should not extend beyond the 18th thoracic vertebra (T18). The study aimed to assess reliability of saddle fitters (SFs) to identify the T18 spinous process (SP). Part 1 investigated agreement between T18 (T18SF) as identified by three SFs using palpation and a veterinary surgeon (VS) using radiography (T18VS) in seven horses. Saddle fitter 1 and SF2 palpated the lumbosacral joint and counted cranially six SPs, whereas SF3 followed the rib curvature toward the dorsal midline. In part 2, SF1 and SF2 identified T18 by counting cranially five S...
A new molecular screening tool for the detection of chromosomal abnormalities in donkeys. Chromosomal abnormalities are a major cause of infertility and reproductive problems in equids. Nowadays, their detection is rising due to the use of new diagnostic tools based on molecular markers instead of karyotyping. Reports of this kind of genetic aberrations in domestic donkeys (Equus asinus) are extremely scarce, despite their importance in human activities. In the present study, we analysed the implementation of a short-tandem-repeat (STR)-based molecular method initially developed for horses, as a diagnostic tool to detect chromosomal abnormalities in donkeys. The frequency of five X...
In silico prediction of prolactin molecules as a tool for equine genomics reproduction. The prolactin hormone is involved in several biological functions, although its main role resides on reproduction. As it interferes on fertility changes, studies focused on human health have established a linkage of this hormone to fertility losses. Regarding animal research, there is still a lack of information about the structure of prolactin. In case of horse breeding, prolactin has a particular influence; once there is an individualization of these animals and equines are known for presenting several reproductive disorders. As there is no molecular structure available for the prolactin hor...
The lipidome of Thoroughbred racehorses before and after supramaximal exercise. A comprehensive study of the effect of supramaximal exercise in lipid homeostasis of Thoroughbreds provides the basis for future research on the role of lipids on energy metabolism in racehorses. Objective: To compare the plasma lipidome of Thoroughbreds before and after supramaximal exercise using an untargeted lipidomics approach. Methods: Pilot experimental study. Methods: Four Thoroughbred horses were used. The maximal oxygen consumption (VO ) was calculated for each horse. Horses then underwent treadmill exercise at the speed for which the oxygen requirements had been calculated to be 11...
Influence of Different Combinations of Permeable and Nonpermeable Cryoprotectants on the Freezing Capacity of Equine Sperm. This study was aimed to evaluate the effect of permeable cryoprotectants in combination with trehalose or sucrose on the freezing capacity of stallion sperm. For this purpose, the ejaculates (n = 24) were collected from four healthy mature Turkmen stallions. The ejaculates were pooled and diluted with one of the extenders containing a combination of 5% of permeating (dimethylacetamide [DMA]; dimethylformamide [DMF] or glycerol) and 50 mM of nonpermeating cryoprotectant agents (CPAs) (sucrose or trehalose) to a final concentration of 200 × 10 spermatozoa/mL. The extended samples were cryopr...
