Analyze Diet

Topic:Fiber

Fiber is an essential component of the equine diet, primarily sourced from forage such as hay and pasture. It plays a significant role in the digestive health of horses by facilitating normal gastrointestinal function and promoting the fermentation process in the hindgut. The breakdown of fiber by microbial populations in the cecum and colon produces volatile fatty acids, which serve as an important energy source for horses. This page compiles peer-reviewed research studies and scholarly articles that explore the types of fiber, their digestibility, and their impact on equine nutrition and gastrointestinal health.
Diet modulates strongyle infection and microbiota in the large intestine of horses.
PloS one    April 9, 2024   Volume 19, Issue 4 e0301920 doi: 10.1371/journal.pone.0301920
Laroche N, Grimm P, Julliand S, Sorci G.The use of anthelminthic drugs has several drawbacks, including the selection of resistant parasite strains. Alternative avenues to mitigate the negative effects of helminth infection involve dietary interventions that might affect resistance and/or tolerance by improving host immunity, modulating the microbiota, or exerting direct anthelmintic effects. The aim of this study was to assess the impact of diet on strongyle infection in horses, specifically through immune-mediated, microbiota-mediated, or direct anthelmintic effects. Horses that were naturally infected with strongyles were fed eit...
Optimization of the Amplification of Equine Muscle-Derived Mesenchymal Stromal Cells in a Hollow-Fiber Bioreactor.
Methods and protocols    April 2, 2024   Volume 7, Issue 2 32 doi: 10.3390/mps7020032
Duysens J, Graide H, Niesten A, Mouithys-Mickalad A, Ceusters J, Serteyn D.The main causes of mortality in horses are the gastrointestinal pathologies associated with septic shock. Stem cells have shown, through systemic injection, a capacity to decrease inflammation and to regenerate injured tissue faster. Nevertheless, to achieve this rapid and total regeneration, systemic injections of 1 to 2 million cells per kilogram of body weight must be considered. Here, we demonstrate for the first time the feasibility and expansion capacity of equine muscle-derived mesenchymal stromal cells (mdMSCs) in a functionally closed, automated, perfusion-based, hollow-fiber bioreact...
Baselining physiological parameters in three muscles across three equine breeds. What can we learn from the horse?
Frontiers in physiology    February 7, 2024   Volume 15 1291151 doi: 10.3389/fphys.2024.1291151
Vidal Moreno de Vega C, de Meeûs d'Argenteuil C, Boshuizen B, De Mare L, Gansemans Y, Van Nieuwerburgh F, Deforce D, Goethals K, De Spiegelaere W....Mapping-out baseline physiological muscle parameters with their metabolic blueprint across multiple archetype equine breeds, will contribute to better understanding their functionality, even across species. Aims: 1) to map out and compare the baseline fiber type composition, fiber type and mean fiber cross-sectional area (fCSA, mfCSA) and metabolic blueprint of three muscles in 3 different breeds 2) to study possible associations between differences in histomorphological parameters and baseline metabolism. Methods: Muscle biopsies [m. pectoralis (PM), m. vastus lateralis (VL) and m. semitendin...
Changes in the nutrient profile and the load of mycotoxins, phytoestrogens, and pesticides in horse pastures during spring and summer in Austria.
Journal of equine veterinary science    November 3, 2023   Volume 131 104958 doi: 10.1016/j.jevs.2023.104958
Son V, Penagos-Tabares F, Hollmann M, Khiaosa-Ard R, Sulyok M, Krska R, Zebeli Q.Pastures are used for grazing and the production of conserved roughage in horses. Yet, the nutritional profile of the forage varies from spring to late summer, affecting equine nutrient supply and health. In addition, environmental factors may also favor plant contaminants such as mycotoxins. This study aimed to determine the nutritional profile and contaminant load of selected horse pastures from early spring till late summer. The nutrient composition (main macronutrients, macro elements and trace elements), as well as mycotoxins, metabolites, pesticides, and plant-derived compounds of seven ...
Percutaneous ultrasonic debridement of equine tendinopathy and desmopathy: A report of 10 cases.
Open veterinary journal    September 30, 2023   Volume 13, Issue 9 1141-1149 doi: 10.5455/OVJ.2023.v13.i9.10
Vlahos TP.Tendinopathy and desmopathy are significant causes of morbidity in horses. To evaluate the use of percutaneous ultrasonic debridement (PUD) as a treatment for chronic tendinopathy and desmopathy in the horse. Eight adult horses with 10 affected limbs presented for lameness, ranging from 60-700 days postinjury. Diagnostic ultrasound identified the following: suspensory branch desmitis ( = 1), suspensory body desmitis ( = 2), Achilles tendinopathy ( = 1), desmitis of the accessory ligament of the deep digital flexor (DDF) tendon ( = 1), DDF tendinopathy ( = 2), and superficial digital flexor ten...
Intravenous Injection of Sodium Hyaluronate Diminishes Basal Inflammatory Gene Expression in Equine Skeletal Muscle.
Animals : an open access journal from MDPI    September 27, 2023   Volume 13, Issue 19 doi: 10.3390/ani13193030
Gregg SR, Barshick MR, Johnson SE.Following strenuous exercise, skeletal muscle experiences an acute inflammatory state that initiates the repair process. Systemic hyaluronic acid (HA) is injected to horses routinely as a joint anti-inflammatory. To gain insight into the effects of HA on skeletal muscle, adult Thoroughbred geldings (n = 6) were injected with a commercial HA product weekly for 3 weeks prior to performing a submaximal exercise test. Gluteal muscle (GM) biopsies were obtained before and 1 h after exercise for gene expression analysis and HA localization. The results from RNA sequencing demonstrate differences in ...
Distribution of equine and food animal emergency caseload by time of day, day of week, time of year, holiday, and phase of moon at an academic ambulatory and referral hospital.
Journal of veterinary emergency and critical care (San Antonio, Tex. : 2001)    September 14, 2023   Volume 33, Issue 6 696-704 doi: 10.1111/vec.13334
Graves MT, Zhu X.Determine the association of time of day, day of week, time of year, holiday, and phase of moon on academic ambulatory and hospital emergency caseload for equine and food animal species. Methods: Retrospective study. Methods: Large animal teaching hospital emergency service. Methods: Equine and food animals. Methods: None. Results: The hospital database was searched for after-hours on-farm and in-hospital emergencies seen from 2014 to 2020. Variables included date and time of admission, species (equine or food/fiber animal [FA]), and hospital service (field or in-hospital). The association of ...
Growth Rates of Thoroughbred Foals and In Vitro Gut Health Parameters When Fed a Cereal or an All-Fiber Creep Feed.
Journal of equine veterinary science    July 15, 2020   Volume 93 103191 doi: 10.1016/j.jevs.2020.103191
Moore-Colyer M, Tuthill P, Bannister I, Daniels S.Traditions and the economic advantage when producing big athletic Thoroughbred (TB) yearlings for sale encourage owners to feed high levels of cereals. Such diets can predispose gastric ulceration and developmental orthopedic diseases, impacting negatively on future careers. This study aimed to (1) determine if an all-fiber creep feed (total mixed fiber ration [TMFR] would sustain equal growth rates in TB foals versus a cereal-based stud cube (SC) and (2) compare pH and lactate produced by SC and TMFR in in vitro foregut and hindgut conditions. Eight TB foals in matched-pairs were fed SC or T...
The effect of supplementing pony diets with yeast on 1. In vivo and in vitro digestibility, faecal pH and particle size.
Animal : an international journal of animal bioscience    June 25, 2020   Volume 14, Issue 12 2481-2492 doi: 10.1017/S1751731120001524
Garber A, Hastie PM, Farci V, Bulmer L, Alzahal O, Murray JMD.Fibre is essential to maintain healthy gut; however, energy demands of performance horses can be too high to be met by forages alone. Yeast may support the function of cellulolytic bacteria to digest fibre. The aim of this work was to determine the effect of an oral supplement (VistaEQ) containing 4% live yeast on the in vitro and in vivo digestibility of high-starch (HS) and high-fibre diets (HF). Eight ponies were used in a 4 × 4 Latin square design consisting of 4- × 19-day periods and four diets: HF, HF + yeast (HFY), HS and HS + yeast (HSY). In vivo apparent digestibility (AD) was estim...
Digestibility and Retention Time of Coastal Bermudagrass (Cynodon dactylon) Hay by Horses.
Animals : an open access journal from MDPI    December 14, 2019   Volume 9, Issue 12 doi: 10.3390/ani9121148
Hansen TL, Chizek EL, Zugay OK, Miller JM, Bobel JM, Chouinard JW, Adkin AM, Skurupey LA, Warren LK.Bermudagrass (Cynodon dactylon) and other warm-season grasses are known for their increased fiber concentrations and reduced digestibility relative to cool-season grasses and legumes. This study investigated the digestive characteristics and passage kinetics of three maturities of Coastal bermudagrass hay. A 5 × 5 Latin square design experiment was used to compare the digestion of five hays: alfalfa (Medicago sativa, ALF), orchardgrass (Dactylis glomerata, ORCH), and Coastal bermudagrass harvested at 4 (CB 4), 6 (CB 6), and 8 weeks of regrowth (CB 8). Horses were fed cobalt-ethylenediaminetet...
Donkey Nutrition and Malnutrition.
The Veterinary clinics of North America. Equine practice    October 3, 2019   Volume 35, Issue 3 469-479 doi: 10.1016/j.cveq.2019.08.004
Burden FA, Bell N.The domestic donkey is a unique equid species with specific nutritional requirements. This article examines the importance of feeding strategies that mimic the donkey's natural environment using poor nutritional quality fibers and access to browsing materials. The relationship between nutrition and health is examined and practical approaches to the healthy and sick donkey are discussed.
Association between nutritional values of hays fed to horses and sensory properties as perceived by human sight, touch and smell.
Animal : an international journal of animal bioscience    February 5, 2019   Volume 13, Issue 9 1834-1842 doi: 10.1017/S1751731118003725
Julliand S, Dacremont C, Omphalius C, Villot C, Julliand V.Although hay is the foundation of most equine diets, horse owners rarely ask for biochemical analysis and the routine practice is to choose hay based on its 'perceived' nutritional value. The present study aimed at exploring the relationship between sensory properties as perceived by sight, touch and smell, and the nutritional value of hay measured by biochemical analysis using a 'free sorting task' method. Fifty-four non-expert participants were asked individually to: (1) observe 21 hays samples, (2) group together hays that they perceived as similar for each of the three modalities (hay appe...
Influence of various concentrate-to-roughage ratios on dietary intake and nutrient digestibilities of weanling horses.
Journal of animal physiology and animal nutrition    October 23, 2018   Volume 103, Issue 1 295-304 doi: 10.1111/jpn.13006
Turcott-White SK, Nielsen BD, Robison CI, Skelly CD, Rosenstein DS, Pritchard A, Herdt T.The objective of this study was to compare the feed intake and the apparent digestibilities of three different diets varying in concentrate-to-roughage ratios in weanling horses (n = 24) at 5 and 8 months of age. Horses were stratified by breed, gender, birth date and body weight and assigned to one of three dietary treatments containing the following concentrate-to-roughage ratios on an as-fed basis: 70:30 (High Con), 50:50 (Equal) and 30:70 (Low Con). All horses were fed their respective diets for a 10-day adaptation period and a 4-day collection period at 5 and 8 months. There were no d...
Impact of barley form on equine total tract fibre digestibility and colonic microbiota.
Animal : an international journal of animal bioscience    August 28, 2015   Volume 9, Issue 12 1943-1948 doi: 10.1017/S1751731115001524
Philippeau C, Sadet-Bourgeteau S, Varloud M, Julliand V.This study aimed at assessing the impact of four barley forms on total tract apparent digestibility of dietary fibre in horses fed a large amount of starch in the morning meal (0.27% BW). Processed barley forms had a greater pre-caecal starch digestibility than the whole form. Based on this result, we hypothesised that using barley-processing methods would limit the potential dumping of undegraded starch in the hindgut of horses and, consequently, the potential negative effect on fibre degradation in the hindgut. In a 4×4 latin square design, four mature geldings fitted with a right ventral c...
Effects of Saccharomyces cerevisiae supplementation on apparent total tract digestibility of nutrients and fermentation profile in healthy horses.
Journal of animal physiology and animal nutrition    May 10, 2013   Volume 97 Suppl 1 115-120 doi: 10.1111/jpn.12043
Mackenthun E, Coenen M, Vervuert I.Supplementation with Saccharomyces cerevisiae (SC) in horses may have some potential to modify microbial populations and thereby improve fibre digestibility. The aim of this study was to investigate the effects of SC on apparent total tract digestibility (ATTD) of nutrients, with a special focus on fibre digestion in healthy horses. The fermentation profile of microbial populations was another focus of interest. Twelve geldings were randomly assigned to three groups. The basal diet consisted of cracked corn (2 g starch/kg body weight [BW]) and hay (1.2 kg/100 kg BW). During adaptation (3 weeks...
Equine pre-caecal and total tract digestibility of individual carbohydrate fractions and their effect on caecal pH response.
Archives of animal nutrition    November 8, 2012   Volume 66, Issue 6 490-506 doi: 10.1080/1745039X.2012.740311
Brøkner C, Austbø D, Næsset JA, Knudsen KE, Tauson AH.The working hypothesis was that a minor postprandial caecal pH decline would affect apparent total tract digestibility (ATTD) of the fibre fraction in horses and, hence, that soluble fibre would amplify fermentation and consequently increase ATTD of fibre. This study was a 4 × 4 Latin Square design with a sequence of 17 days adaptation to the ration followed by 8 sampling days. The feed rations consisted of only timothy hay (Group H), hay plus molassed sugar beet pulp combined with either whole oats (Group OB) or barley (Group BB) and hay plus loose chaff based concentrate (Group M). Four hor...
A comparative study of the apparent total tract digestibility of carbohydrates in Icelandic and Danish warmblood horses fed two different haylages and a concentrate consisting of sugar beet pulp and black oats.
Archives of animal nutrition    December 1, 2010   Volume 64, Issue 5 343-356 doi: 10.1080/1745039X.2010.504606
Jensen RB, Brokner C, Knudsen KE, Tauson AH.Four Icelandic (ICE) and four Danish Warmblood (DW) horses were used in a crossover study with two treatments to investigate the effect of breed and the effect of stage of maturity of haylage on the apparent total tract digestibility (ATTD) of a diet consisting of sugar beet pulp, black oats and haylage early or late cut. Fibre was analysed as crude fibre (CF), acid detergent fibre (ADF), neutral detergent fibre (NDF) and dietary fibre (DF = non-starch polysaccharides (NSP) plus lignin). In haylage all analysed fibre fractions increased with advancing stage of maturity, with the cell wall comp...
Motivation for hay: effects of a pelleted diet on behavior and physiology of horses.
Physiology & behavior    September 24, 2010   Volume 101, Issue 5 623-627 doi: 10.1016/j.physbeh.2010.09.010
Elia JB, Erb HN, Houpt KA.The natural diet of free-ranging horses is grass, which is typically high in fiber and calorically dilute, however diets for high performance domestic horses are often low in fiber and calorically dense. The aim of the study was to determine the motivation of horses for hay when fed a low roughage diet. Their motivation could be used to determine if low roughage diets compromise the welfare of horses. Eight mares were fed two different diets in counterbalanced order: ad libitum orchard grass hay; a complete pelleted feed (pellets). Each trial lasted three weeks, with a one-week transition peri...
Effects of diet and weight gain on circulating tumour necrosis factor-α concentrations in Thoroughbred geldings.
Journal of animal physiology and animal nutrition    July 30, 2010   Volume 95, Issue 2 161-170 doi: 10.1111/j.1439-0396.2010.01036.x
Suagee JK, Burk AO, Quinn RW, Hartsock TG, Douglass LW.Low-grade inflammation precedes the development of obesity-related metabolic disorders in humans, but whether the same is true in the horse is not known. The objective of this study was to examine the effects of weight gain and diet on the inflammatory state of horses as determined by serum concentrations of tumour necrosis factor-α (TNF), an inflammatory cytokine. Fifteen mature Thoroughbred geldings with an initial body weight (BW) of 519±12 kg and body condition score (BCS) of 4.3±0.1 were fed a diet of hay plus a concentrate that was either high in non-structural carbohydrates (NSC) (i....
The development of a metabolizable energy system for horses.
Journal of animal physiology and animal nutrition    July 16, 2010   Volume 94, Issue 6 e231-e240 doi: 10.1111/j.1439-0396.2010.01015.x
Kienzle E, Zeyner A.The development of a metabolizable energy (ME) system for horses is described. Predictive equations for gross energy and digestible energy (DE) are revisited. The relationship between feed protein content and renal energy losses and the relationship between feed fibre content and methane energy losses were analysed in a literature review to develop predictive equations for ME. In horses, renal energy losses are much higher than losses by methane energy. Renal energy losses were correlated more strictly to protein intake than to digestible protein intake. The reason probably is that per gram of...
Role of diet and feeding in normal and stereotypic behaviors in horses.
The Veterinary clinics of North America. Equine practice    March 24, 2009   Volume 25, Issue 1 167-viii doi: 10.1016/j.cveq.2009.01.002
Hothersall B, Nicol C.This article reviews the effects of diet on equine feeding behavior and feeding patterns, before considering the evidence that diet affects reactivity in horses. A growing body of work suggests that fat- and fiber-based diets may result in calmer patterns of behavior, and possible mechanisms that may underpin these effects are discussed. In contrast, there is little evidence that herbal- or tryptophan-containing supplements influence equine behavior in any measurable way. The role of diet in the development of abnormal oral behaviors, particularly the oral stereotypy crib-biting, is also revie...
The effect of mixing and changing the order of feeding oats and chopped alfalfa to horses on: glycaemic and insulinaemic responses, and breath hydrogen and methane production.
Journal of animal physiology and animal nutrition    August 12, 2008   Volume 93, Issue 5 631-638 doi: 10.1111/j.1439-0396.2008.00848.x
Vervuert I, Voigt K, Hollands T, Cí·¯ord D, Coenen M.The aim of this study was to investigate the effects of feeding oats alone before or after feeding chopped alfalfa or, in admixture with the alfalfa on the glycaemic and insulinaemic responses of horses as well as post-prandial breath hydrogen and methane excretion. Horses were fed in a randomized order, chopped alfalfa as a source of dietary fibre and unprocessed oats as a source of starch. Chopped alfalfa intake was adjusted to a crude fibre intake of 0.5 g/kg bodyweight (BW) per meal and the oats intake was adjusted to a starch intake of 2 g/kg BW per meal. The feeds were offered in three d...
Effect of live yeast culture supplementation on apparent digestibility and rate of passage in horses fed a high-fiber or high-starch diet.
Journal of animal science    October 2, 2007   Volume 86, Issue 2 339-347 doi: 10.2527/jas.2006-796
Jouany JP, Gobert J, Medina B, Bertin G, Julliand V.Eight crossbred male horses aged 12 +/- 5 yr and with BW of 305 +/- 18 kg were used in pairs in a 4 x 4 Latin square design with 4 ground and pelleted diets. Each pair included a cecum and right ventral colon-fistulated animal and a cecal-fistulated animal. The 4 horse diets were a high-fiber diet (HF+0) based on dehydrated alfalfa, a high-starch diet based on barley and wheat bran (HS+0), and the HF or HS diets supplemented with Saccharomyces cerevisiae (SC) CBS 493.94 (HF+SC and HS+SC). The probiotic preparation contained 4.5 x 10(9) cfu/g of live yeast mixed with the culture medium, and was...
Soybean hulls as an alternative feed for horses.
Journal of animal science    June 26, 2004   Volume 82, Issue 6 1663-1668 doi: 10.2527/2004.8261663x
Coverdale JA, Moore JA, Tyler HD, Miller-Auwerda PA.Soybean hulls have been successfully fed to ruminant animals as an economical substitute for hay. This feedstuff is a source of highly digestible fiber that does not contain starch. The purpose of this trial was to evaluate soybean hulls as a replacement fiber in horse diets. Four cecally cannulated Quarter Horse geldings, aged 6 to 10 yr and averaging 502 kg, were used in a 4 x 4 Latin square design with 21-d periods. Diets consisted of alfalfa/bromegrass hay (14.4% CP, 58.1% NDF, 39.1% ADF; DM basis) with the replacement of either 0, 25, 50, or 75% (as-fed basis) unpelleted soybean hulls (13...
Effect of a preparation of Saccharomyces cerevisiae on microbial profiles and fermentation patterns in the large intestine of horses fed a high fiber or a high starch diet.
Journal of animal science    November 5, 2002   Volume 80, Issue 10 2600-2609 doi: 10.2527/2002.80102600x
Medina B, Girard ID, Jacotot E, Julliand V.Eight horses were allotted into pairs consisting of one cecum- and right ventral colon-fistulated animal and one cecum-fistulated animal. They were fed daily at the same level of intake either a high-fiber (HF) or a high-starch (HS) diet without or with 10 g of a Saccharomyces cerevisiae preparation, in a 4 x 4 Latin square design. The HS diet provided a starch overload (i.e., 3.4 g starch x kg(-1) BW x meal(-1)) while maintaining a high amount of fiber intake (i.e., dietary NDF/starch ratio was 1.0). A 21-d period of adaptation to the treatments occurred before cecal and colonic contents were...
Brewing by-products: their use as animal feeds.
The Veterinary clinics of North America. Food animal practice    September 19, 2002   Volume 18, Issue 2 233-252 doi: 10.1016/s0749-0720(02)00016-6
Westendorf ML, Wohlt JE.Brewers grains, a by-product of beer production, are often used as a livestock feed. Because brewers grains provide protein, fiber, and energy, they can be useful in a variety of diets. Protein in brewers grains can meet a significant portion of supplemental protein requirements; in addition, they provide fiber and needed bulk in the diets of ruminants and horses. Brewers grains and other brewers by-products have also been fed to pigs, sheep, and poultry. Currently, the primary market for wet brewers grains is as a dairy cattle feed; however, some may be fed to beef cattle in feedlots. Brewers...
Dietary soyabean oil depresses the apparent digestibility of fibre in trotters when substituted for an iso-energetic amount of corn starch or glucose.
Equine veterinary journal    July 11, 2002   Volume 34, Issue 3 302-305 doi: 10.2746/042516402776186074
Jansen WL, Geelen SN, van der Kuilen J, Beynen AC.The aim of the present study was to establish whether the inhibitory effect of fat feeding on fibre digestion has been underestimated due to the substitution of fat for corn starch. A high fat intake has been shown to lower total intestinal tract apparent digestibility of crude fibre in horses but, since fat was substituted for nonstructural carbohydrates, including starch, the specific effect of fat could not be ascertained. The possibility could not be excluded that starch also inhibits fibre digestibility, so that the fat effect observed earlier would have been underestimated. In this study...
The apparent digestibility of fibre in trotters when dietary soybean oil is substituted for an iso-energetic amount of glucose.
Archiv fur Tierernahrung    March 30, 2002   Volume 54, Issue 4 297-304 doi: 10.1080/17450390109381986
Jansen WL, van der Kuilen J, Geelen SN, Beynen AC.An attempt was made to quantify the effect of extra fat intake on fibre utilization in horses. In a 4 x 4 cross-over trial with feeding periods of 24 days each, eight mature trotting horses (age 4 to 12 years, 407 to 531 kg BW) were given four diets. The concentrates were formulated to contain either soybean oil or an iso-energetic amount of glucose or combinations of the two ingredients. The concentrates were fed in combination with the same amount of hay so that the whole diets contained 30, 50, 77 or 108 g EE/kg DM. Apart from the amounts of fat and glucose the four diets were identical. Wi...
Apparent digestibility of crude fibre in ponies fed either a low or high-protein diet.
Journal of animal physiology and animal nutrition    November 1, 2001   Volume 85, Issue 7-8 251-254 doi: 10.1046/j.1439-0396.2001.00323.x
Rey F, Hallebeek JM, Beynen AC.The question addressed was whether apparent crude fibre digestibility in ponies would change after lowering protein intake from adequate to borderline deficient. Four adult ponies were fed a low- and high-protein diet according to a cross-over design. The diets consisted of grass hay and concentrates and provided either 1.5 or 3.6 g digestible crude protein/kg(0.75) per day. The two whole rations provided 2.4 g crude fibre/kg body weight per day. Apparent crude fibre digestibility was not affected by protein intake (low-protein diet: 42.9 +/- 4.03%; high-protein diet: 38.1 +/- 1.14%, means +/-...
The effect of replacing nonstructural carbohydrates with soybean oil on the digestibility of fibre in trotting horses.
Equine veterinary journal    February 8, 2000   Volume 32, Issue 1 27-30 doi: 10.2746/042516400777612008
Jansen WL, van der Kuilen J, Geelen SN, Beynen AC.The hypothesis tested was that the intake of extra fat at the expense of an isoenergetic amount of nonstructural carbohydrates reduces fibre utilisation in horses. In a crossover trial with feeding periods of 42 days each, 6 mature trotting horses (age 4-12 years, bodyweight 340-476 kg) were given either a control or test diet. The test concentrate was formulated to contain 37% of net energy in the form of soybean oil. The control concentrate contained an isoenergetic amount of corn starch plus glucose. The concentrates were fed in combination with the same amount of hay so that the control an...