Analyze Diet

Topic:Food Safety

Food safety in horses encompasses practices and standards aimed at ensuring the health and well-being of horses through the management of their dietary intake. This includes the assessment and control of potential contaminants and pathogens in equine feed and water sources. Key concerns in equine food safety include the presence of mycotoxins, pesticide residues, and bacterial pathogens such as Salmonella and Clostridium botulinum. Proper feed storage, sourcing, and preparation are integral to preventing foodborne illnesses in horses. This page compiles peer-reviewed research studies and scholarly articles that explore the detection, impact, and management of food safety issues in equine nutrition.
Toxicological effects of aflatoxins in horses.
Veterinary journal (London, England : 1997)    July 8, 2010   Volume 188, Issue 3 270-273 doi: 10.1016/j.tvjl.2010.06.002
Caloni F, Cortinovis C.Aflatoxins are a group of mycotoxins principally produced by Aspergillus flavus and A. parasiticus, which are both natural contaminants of food and feedstuff. Aflatoxin B(1) is the most prevalent member of this group that is normally detected and is the most powerful hepatocarcinogen known. Few naturally occurring episodes of aflatoxicosis in horses have been reported in the literature. Indeed, the published information about aflatoxin exposure, metabolism and the effects on horses is limited and controversial, possibly indicating a lack of awareness rather than the rarity of the occurrence. T...
Determination of non-steroidal anti-inflammatory drugs residues in animal muscles by liquid chromatography-tandem mass spectrometry.
Analytica chimica acta    April 24, 2010   Volume 672, Issue 1-2 85-92 doi: 10.1016/j.aca.2010.04.031
Jedziniak P, Szprengier-Juszkiewicz T, Olejnik M, Zmudzki J.A confirmatory method for the determination of residues of nine non-steroidal anti-inflammatory drugs and one metabolite in animal muscles has been developed. After enzymatic hydrolysis samples were extracted with acetonitrile and cleaned up using alumina and C(18) SPE cartridges. Liquid chromatography-tandem mass spectrometry was used for the separation and determination of analytes. The method was validated in bovine muscles, according to the Commission Decision 2002/657/EC criteria. Applicability of the method in the analysis of swine, horse and chicken muscles was checked by precision and ...
Improving the sensitivity of Salmonella testing in horses: how good is good enough?
Veterinary journal (London, England : 1997)    February 13, 2010   Volume 187, Issue 2 147-148 doi: 10.1016/j.tvjl.2010.01.003
McKenzie HC, Hodgson JL.No abstract available
The origin and foot condition of horses slaughtered in Australia for the human consumption market.
Equine veterinary journal    January 26, 2010   Volume 41, Issue 8 808-811 doi: 10.2746/042516409x434071
Doughty A, Cross N, Robins A, Phillips CJ.There are ethical concerns regarding the loss of horses from various equine industries and their corresponding slaughter. However, to date, no empirical evidence regarding the extent of this loss, nor of the condition of horses involved, exists within Australia. Objective: To determine the approximate ages, brand type and condition of feet of horses relinquished to an export abattoir in Australia. Methods: Data were collected from 340 horses processed at an Australian abattoir from November 2007-January 2008. Foot abnormalities, injuries and hoof indicators of overgrown and untrimmed hooves we...
Microbiological sampling of carcasses by excision or swabbing with three types of sponge or gauze.
Journal of food protection    January 7, 2010   Volume 73, Issue 1 81-87 doi: 10.4315/0362-028x-73.1.81
Martínez B, Celda MF, Anastasio B, García I, López-Mendoza MC.Fifty-five bovine, 50 equine, 60 ovine, and 50 porcine carcasses were sampled in a slaughterhouse in eastern Spain. Two samples were taken from each carcass, one using the excision method and the other using the swabbing method. Four different materials were used for swabbing: cellulose, polyurethane, or viscose sponges, and medical gauze. Samples were collected at the end of the process by four different people before the carcasses were taken to the cooler. The samples were examined for total viable bacteria counts (TVCs) and Enterobacteriaceae counts (ECs). The mean TVC for all species sampl...
Detection of prohibited animal products in livestock feeds by single-strand conformation polymorphism analysis.
Journal of food protection    January 7, 2010   Volume 73, Issue 1 119-124 doi: 10.4315/0362-028x-73.1.119
Huby-Chilton F, Murphy J, Chilton NB, Gajadhar AA, Blais BW.Single-strand conformation polymorphism (SSCP) analysis of amplicons produced from a mitochondrial DNA region between the tRNA(Lys) and ATPase8 genes was applied for the detection of animal product within livestock feeds. Identification of prohibited animal (cattle, elk, sheep, deer, and goat) and nonprohibited animal (pig and horse) products from North America was possible based on the differential display of the single-stranded DNA fragments for the different animal species on SSCP gels. This method allowed specific detection and identification of mixed genomic DNA from different animal spec...
Aspergillus fumigatus toxicity and gliotoxin levels in feedstuff for domestic animals and pets in Argentina.
Letters in applied microbiology    November 6, 2009   Volume 50, Issue 1 77-81 doi: 10.1111/j.1472-765X.2009.02756.x
Pena GA, Pereyra CM, Armando MR, Chiacchiera SM, Magnoli CE, Orlando JL, Dalcero AM, Rosa CA, Cavaglieri LR.To evaluate gliotoxin production by Aspergillus fumigatus strains isolated from feedstuff intended for domestic animals and pets, and to determine the amount of gliotoxin in these substrates. Results: A total of 150 feedstuff samples were collected. They were composed of 30 samples each of five different feed types (pigs, poultry, cattle, horse and pets). Aspergillus fumigatus gliotoxin production ability and gliotoxin presence in feedstuff was determined by HPLC. Aspergillus fumigatus strains were isolated from all of the tested samples. Strains from cattle, horses and pet food were able to p...
Trichinella britovi and Trichinella spiralis mixed infection in a horse from Poland.
Veterinary parasitology    January 24, 2009   Volume 161, Issue 3-4 345-348 doi: 10.1016/j.vetpar.2009.01.013
Liciardi M, Marucci G, Addis G, Ludovisi A, Gomez Morales MA, Deiana B, Cabaj W, Pozio E.Trichinella infections in horses continue to represent a health problem and, despite the rarity of infection, it is necessary to continue to control properly horse meat. In 2008, a 10-year-old horse imported from Poland to Italy for consumption found to have been positive at the digestion test. Both Trichinella britovi and Trichinella spiralis larvae in a proportion of 4:1 were detected in the horse muscles. This is the first report of a mixed Trichinella species infection in a horse. The epidemiological investigation revealed that the infected horse originated from a small farm about 120km fr...
Trichinella spp. infection in horses of Romania: serological and parasitological survey.
Veterinary parasitology    October 22, 2008   Volume 159, Issue 3-4 285-289 doi: 10.1016/j.vetpar.2008.10.058
Blaga R, Cretu CM, Gherman C, Draghici A, Pozio E, Noeckler K, Kapel CM, Dida I, Cozma V, Boireau P.Herbivorous animals are usually, by virtue of their diet, outside the major transmission cycles of Trichinella spp. However, since 1975, the year of the first report of human trichinellosis caused by the consumption of infected horse meat, the domestic horse has appeared as a novel vector of Trichinella spp. infection to humans, with 15 outbreaks documented in France and Italy. Romania, one of the main countries exporting horses into the European Union (EU), experienced a dramatic increase of Trichinella spp. infection in both domestic pigs and humans in the 1990s. Some Trichinella spiralis-in...
Risk factors for Campylobacter jejuni and Campylobacter coli in young cattle on English and Welsh farms.
Preventive veterinary medicine    October 2, 2008   Volume 88, Issue 1 42-48 doi: 10.1016/j.prevetmed.2008.07.002
Ellis-Iversen J, Pritchard GC, Wooldridge M, Nielen M.Campylobacter jejuni and C. coli are the most prevalent causes of bacterial diarrhoea in most of the Western World. In Great Britain, the source remains unknown for the majority of cases, though poultry is considered the main source of infection. Molecular typing methods identify cattle as a potential source of a proportion of the non-source-attributed cases, mainly through direct contact, environmental contamination or milk, but little is known about the epidemiology of Campylobacter in cattle. A cross-sectional study was undertaken on young cattle 3-17 months of age on 56 cattle farms in Eng...
Methicillin-resistant Staphylococcus aureus and animals: zoonosis or humanosis?
The Journal of antimicrobial chemotherapy    September 26, 2008   Volume 62, Issue 6 1181-1187 doi: 10.1093/jac/dkn405
Morgan M.Methicillin-resistant Staphylococcus aureus (MRSA) is increasing worldwide. Occasionally, animals are colonized or infected incidentally with human strains. Recently, however, new strains of MRSA emerging from within the animal kingdom, particularly in pigs, are causing human infection. MRSA has been reported in species as diverse as companion animals, horses and pigs, through to chinchillas, bats and parrots. In contrast, whereas strains of community-associated MRSA, the majority of which carry genes encoding Panton-Valentine leucocidin, are spreading rapidly in human populations, only sporad...
Filarioid nematodes in cattle, sheep and horses in Finland.
Acta veterinaria Scandinavica    June 16, 2008   Volume 50, Issue 1 20 doi: 10.1186/1751-0147-50-20
Solismaa M, Laaksonen S, Nylund M, Pitkänen E, Airakorpi R, Oksanen A.In autumn 2006, Finnish meat inspection data revealed lesions in tendons, muscles and ligaments of bovine hind legs leading to partial condemnation of carcasses. In gross pathological examination at Finnish Food Safety Authority Evira, Oulu (now Fish and Wildlife Health) Research Unit, Onchocerca sp. (Filarioidea; Onchocercidae) nematodes were detected in lesions. Due to this, a pilot study was made in order to find out what filarioid nematodes do occur in cattle, horses and sheep in Finland. Methods: Ventral skin biopsies from 209 dairy cattle and 42 horses, as well as blood samples from 209 ...
Species-specific concentrations of perfluoroalkyl contaminants in farm and pet animals in Japan.
Chemosphere    April 24, 2008   Volume 73, Issue 1 Suppl S210-S215 doi: 10.1016/j.chemosphere.2006.12.105
Guruge KS, Manage PM, Yamanaka N, Miyazaki S, Taniyasu S, Yamashita N.The persistent metabolites of perfluorinated compounds (PFCs) which have been detected in the tissues of both humans and wildlife, and human contamination by PFCs suggest differences in the exposure patterns to these compounds. However, studies focused on identifying human exposure pathways to PFCs are scarce. To provide a preliminary assessment of PFCs in farm animals such as chicken, cattle, pigs, goats and horses, blood and liver samples were collected from various regions in Japan. Additionally, dog sera samples representing pet animals were also employed for analysis. Perfluorooctane sulf...
Physicochemical properties of meat of Italian Heavy Draft horses slaughtered at the age of eleven months.
Journal of animal science    February 1, 2008   Volume 86, Issue 5 1205-1214 doi: 10.2527/jas.2007-0629
Tateo A, De Palo P, Ceci E, Centoducati P.To investigate rheological and chemical characteristics of the meat from Italian Heavy Draft horse, 24 foals (12 males and 12 females) were weaned at 6 mo, reared and fed in the same way, and slaughtered at 11 mo of age. The results obtained showed that there were no significant differences between the sexes but that the muscle type is a significant variation source. The rectus femoris muscle was lighter, and the biceps femoris had a lower a* index than longissimus dorsii, rectus femoris, and semimembranosus muscle. The most tender muscle was the semitendinosus, and the toughest even after coo...
VanA-type vancomycin-resistant enterococci in equine and swine rectal swabs and in human clinical samples.
Current microbiology    July 25, 2007   Volume 55, Issue 3 240-246 doi: 10.1007/s00284-007-0115-0
de Niederhäusern S, Sabia C, Messi P, Guerrieri E, Manicardi G, Bondi M.Vancomycin-resistant enterococci (VRE) in healthy people and in food-producing animals seems to be quite common in Europe. The existence of this community reservoir of VRE has been associated with the massive use of avoparcin in animal husbandry. Eight years after the avoparcin ban in Europe, we investigated the incidence of VanA enterococci, their resistance patterns, and the mobility of their glycopeptide-resistance determinants in a sampling of animal rectal swabs and clinical specimens. A total of 259 enterococci isolated from equine, swine, and clinical samples were subcultured on KF-stre...
Sodium lactate influences myoglobin redox stability in vitro.
Meat science    July 17, 2007   Volume 78, Issue 4 529-532 doi: 10.1016/j.meatsci.2007.07.010
Mancini RA, Ramanathan R.Injection-enhancement of beef with lactate improves color stability; however, the mechanism is unclear. Thus, our objectives were to assess the effects of sodium lactate on equine myoglobin redox stability in vitro. Oxymyoglobin at pH 5.6 (50mM sodium citrate) and pH 7.4 (50mM sodium phosphate) was incubated at 4°C with lactate (0, 5, 10, 100, or 200mM) and myoglobin redox form was determined using absorbance spectra. Metmyoglobin formation at pH 5.6 and 7.4 was significantly (P<0.05) decreased by lactate at concentrations of 100 and 200mM. In general, increasing lactate concentration from...
On the presence of antibodies against bovine, equine and poultry immunoglobulins in human IgG preparations, and its implications on antivenom production.
Toxicon : official journal of the International Society on Toxinology    June 26, 2007   Volume 51, Issue 1 10-16 doi: 10.1016/j.toxicon.2007.06.009
Sevcik C, Díaz P, D'Suze G.Specific immunoassays were developed to detect anti-horse, anti-chicken and anti-bovine immunoglobulins in human IgG preparations. Three samples of 5% human IgG for intravenous use ("Inmunoglobulina G Endovenosa al 5%"(trade mark), Quimbiotec CA), were studied. All samples were produced from pools of >2500 plasma units from different donors. One sample was produced from an only Venezuelan plasma pool (2660 donors) and the other two were produced from a 1:1 blend of Venezuelan and Canadian plasma pools. The amounts of human IgG detected were 0.017 (0.015,0.020) mg/ml (n=18) against horse IgG...
Viability and infectivity of Trichinella spiralis muscle larvae in frozen horse tissue.
Veterinary parasitology    April 5, 2007   Volume 146, Issue 1-2 102-106 doi: 10.1016/j.vetpar.2007.02.001
Hill DE, Forbes L, Gajadhar AA, Gamble HR.Many aspects of the biology and epidemiology of Trichinella infection in the horse are poorly understood, including survival of Trichinella spp in horse muscle. In this study, we have assessed the freeze tolerance of T. spiralis in horse meat stored at 5, -5, and -18 degrees C for 1 day to 24 weeks. Results demonstrate a steady reduction in the number of live ML recovered from the cold stored meat samples. On Day 1, recovery of live larvae had been reduced by 18.6%, 50.1%, and 37.2%, and by 4 weeks, recovery of larvae had been reduced by 65.4%, 66.5%, and 96.2% in samples stored at 5, -5, and ...
Larval viability and serological response in horses with long-term Trichinella spiralis infection.
Veterinary parasitology    March 26, 2007   Volume 146, Issue 1-2 107-116 doi: 10.1016/j.vetpar.2007.02.011
Hill DE, Forbes L, Kramer M, Gajadhar A, Gamble HR.The horse is considered an aberrant host for the nematode parasite Trichinella spiralis, and many aspects of the biology and epidemiology of Trichinella infection in the horse are poorly understood. It has been reported that experimentally-infected horses produce a transient serological response to infection and that muscle larvae are cleared more rapidly than in parasite-adapted hosts such as the pig and humans. However, limited numbers of animals have been studied, and both the longevity of larvae in horse musculature and the immune response to Trichinella larvae remain unclear. In this stud...
Identifying the future needs for long-term USDA efforts in agricultural animal genomics.
International journal of biological sciences    February 10, 2007   Volume 3, Issue 3 185-191 doi: 10.7150/ijbs.3.185
Green RD, Qureshi MA, Long JA, Burfening PJ, Hamernik DL.Agricultural animal research has been immensely successful over the past century in developing technology and methodologies that have dramatically enhanced production efficiency of the beef, dairy, swine, poultry, sheep, and aquaculture industries. In the past two decades, molecular biology has changed the face of agricultural animal research, primarily in the arena of genomics and the relatively new offshoot areas of functional genomics, proteomics, transcriptomics, metabolomics and metagenomics. Publication of genetic and physical genome maps in the past 15 years has given rise to the possib...
The Horse Slaughter Act revisited.
Journal of the American Veterinary Medical Association    January 30, 2007   Volume 230, Issue 2 187 
Ward GM.No abstract available
The Horse Slaughter Act revisited.
Journal of the American Veterinary Medical Association    January 30, 2007   Volume 230, Issue 2 187-188 
Thompson BS.No abstract available
[Veterinarians–notables of the cities and the country. Some stages in their social ascent from the second half of the 18th century to the start of the 20th century]. Theves G.During the second half of the 18th century scientific veterinary medicine, a new profession was born as a result of economic and military needs--losses of cattle as a consequence of infectious diseases and the growing demand for treatment to be given to the horses of the armies. At first the members of the emerging occupation, who are generally of very modest origin, hardly differ from the traditional actors of animal health. At the beginning of the 19th century the governments employ a large number of veterinarians for official missions to combat infectious diseases. As from the second half o...
Re: AVMA position on horse slaughter.
Journal of the American Veterinary Medical Association    December 14, 2006   Volume 229, Issue 11 1720-1721 
Marek K.No abstract available
Differentiation of Meat Samples from Domestic Horses (Equus caballus) and Asiatic Wild Asses (Equus hemionus) Using a Species-Specific Restriction Site in the Mitochondrial Cytochrome b Region.
Mongolian journal of biological sciences    December 1, 2006   Volume 4, Issue 2 57-62 doi: 10.22353/mjbs.2006.04.16
Kuehn R, Kaczensky P, Lkhagvasuren D, Pietsch S, Walzer C.Recent studies suggest that Asiatic wild asses (Equus hemionus) are being increasingly poached in a commercial fashion. Part of the meat is believed to reach the meat markets in the capital Ulaanbaatar. To test this hypothesis, we collected 500 meat samples between February and May 2006. To differentiate between domestic horse (Equus caballus) and wild ass meat, we developed a restriction fragment length polymorphism (RFLP) assay based on the polymerase chain reaction (PCR). We amplified and sequenced a cytochrome b fragment (335 bp) and carried out a multialignment of the generated sequences ...
Diversity and distribution of Escherichia coli genotypes and antibiotic resistance phenotypes in feces of humans, cattle, and horses.
Applied and environmental microbiology    September 1, 2006   Volume 72, Issue 11 6914-6922 doi: 10.1128/AEM.01029-06
Anderson MA, Whitlock JE, Harwood VJ.Escherichia coli is the most completely characterized prokaryotic model organism and one of the dominant indicator organisms for food and water quality testing, yet comparatively little is known about the structure of E. coli populations in their various hosts. The diversities of E. coli populations isolated from the feces of three host species (human, cow, and horse) were compared by two subtyping methods: ribotyping (using HindIII) and antibiotic resistance analysis (ARA). The sampling effort required to obtain a representative sample differed by host species, as E. coli diversity was consis...
[Fat and content of yakutan horse fat tissue].
Voprosy pitaniia    April 12, 2006   Volume 74, Issue 6 17-23 
Mordovskaia VI, Krivoshapkin VG, Pogozheva AV, BaÄ­kov VG.The gas chromatography method the fat acid content of the young Yakut horse meat fat tissue lipids was studied in relation to the age group, various parts of the body and anatomic topographic location of the fat/It was determined that the of sixth-month young horse is the source of the PUFA omega-3 that allows to recommend horse meat as a dietary product for atherosclerosis prevention. By medial chain fat content the lipids of the 6 month young horses are close to the lipids of milk fat.
Comparison of hydrolytic and conjugative biotransformation pathways in horse, cattle, pig, broiler chick, rabbit and rat liver subcellullar fractions.
Veterinary research communications    January 27, 2006   Volume 30, Issue 3 271-283 doi: 10.1007/s11259-006-3247-y
Gusson F, Carletti M, Albo AG, Dacasto M, Nebbia C.To complete a studyaimed at investigating the pattern of the basal activities of liver xenobioticmetabolizing enzymes in major and minor species intended for meat production, microsomal carboxylesterases and some conjugating enzyme activities were determined and compared in liver preparations from horses, cattle, pigs, rabbits and broiler chicks, using the rat as a reference species. Horses and broiler chicks exhibited a lower microsomal carboxylesterase activity towards indophenyl or p-nitrophenyl acetate than that measured in cattle or pig subfractions. Among food-producing species, the rate...
[Prediction of 137Cs accumulation in animal products in the territory of Semipalatinsk test site].
Radiatsionnaia biologiia, radioecologiia    October 8, 2005   Volume 45, Issue 4 480-487 
Spiridonov SI, Gontarenko IA, Mukusheva MK, Fesenko SV, Semioshkina NA.The paper describes mathematical models for 137Cs behavior in the organism of horses and sheep pasturing on the bording area to the testing area "Ground Zero" of the Semipalatinsk Test Site. The models are parameterized on the base of the data from an experiment with the breeds of animals now commonly encountered within the Semipalatinsk Test Site. The predictive calculations with the models devised have shown that 137Cs concentrations in milk of horses and sheep pasturingon the testing area to "Ground Zero" can exceed the adopted standards during a long period of time.
Fast food and fat fillies: the ills of western civilisation.
Veterinary journal (London, England : 1997)    September 9, 2005   Volume 172, Issue 3 396-397 doi: 10.1016/j.tvjl.2005.06.009
Sillence M, Noble G, McGowan C.No abstract available