Analyze Diet

Topic:Livestock

Livestock and horses are integral components of agricultural systems, providing resources such as meat, milk, fiber, and labor. Horses, in particular, have unique roles in agriculture, sport, and recreation, distinguishing them from other livestock. This topic explores the management, breeding, nutrition, and health of horses and other livestock species. It encompasses studies on husbandry practices, welfare considerations, and the impact of these animals on the environment and economy. The page includes peer-reviewed research studies and scholarly articles that investigate various aspects of livestock and equine science, offering insights into their biological, ecological, and economic significance.
Species Extinction, Infrastructure Development and Epidemics: The Changing Ecology of African Horsesickness in the Cape Colony, c.1653-1900.
Journal of southern African studies    July 15, 2025   Volume 50, Issue 6 1037-1057 doi: 10.1080/03057070.2024.2508570
Andreas C.The virus that causes African horsesickness does not affect any indigenous species, but produces high mortality among horses, a species introduced by the Dutch East India Company in 1653. While the insect-borne disease did not occur in the immediate vicinity of the Cape Peninsula, horsesickness could have constituted an endemic disease barrier to the horse-based expansion of the colonial sphere into the hinterland, where it was seasonally prevalent. That it did so to only a limited extent is due to a substantial alteration of the ecology of the disease that largely resulted from inadvertent si...
Fasciolosis in Algerian livestock: Epidemiological trends and economic consequences.
Journal of helminthology    July 11, 2025   Volume 99 e77 doi: 10.1017/S0022149X25100503
Fennouh C, Mokrani D, Dahmani H, Salhi O, Ouchetati I, Nabi M, Aouina K, Chettih I, Ouchene N, Khelifi Touhami NA.Fasciolosis, a parasitic disease of ruminants, poses significant economic and animal-health challenges in Algeria. This study aimed to assess spatial, temporal, and species-specific patterns of fasciolosis prevalence across diverse agro-climatic zones and to estimate associated economic losses. Between 2013 and 2023, eight wilayas El-Tarf, Skikda, Jijel (Region I), Blida, Mila (Region II), and M'Sila, Medea, Laghouat (Region III) were surveyed. Systematic postmortem inspections of 1,569,392 animals (349,176 cattle; 982,669 sheep; 235,639 goats; 1,882 camels; 26 horses) were performed by qualif...
Plant trait responses to herbivore type managed as domestic or as wild.
Journal of environmental management    July 7, 2025   Volume 391 126378 doi: 10.1016/j.jenvman.2025.126378
Mutillod C, Buisson E, Tatin L, Mahy G, Dufrêne M, Morvan N, Mesléard F, Dutoit T.The urgency of restoring ecosystems over vast areas has placed rewilding using wild herbivores at the forefront. However, few scientific studies address its effects on biodiversity and ecosystem functioning compared to more traditional conservation interventions with domestic herbivores. Equus ferus przewalskii horses introduced 30 years ago in the National Park of Cévennes, France - as a step of a conservation program of the species for its reintroduction in Mongolia - now occur as a semi-wild horse population, socially natural (i.e., management is based on natural selection). This introduct...
The reemergence of the New World screwworm and its potential distribution in North America.
Scientific reports    July 3, 2025   Volume 15, Issue 1 23819 doi: 10.1038/s41598-025-04804-9
Valdez-Espinoza UM, Fadda LA, Marques R, Osorio-Olvera L, Jiménez-García D, Lira-Noriega A.The reemergence of the New World screwworm (Cochliomyia hominivorax) poses a significant threat to animal and public health with minimal regulatory oversight. This study analyzes the potential distribution and reemergence of this pest, which is endemic to South America but was previously eradicated in North America. We first developed bioclimatic suitability models, and then incorporated these findings along with reemergence records and inspection point data to simulate possible dispersal routes into Mexico and the United States. Our results document the historical distribution of C. hominivor...
First case report of Corynebacterium pseudotuberculosis biovar equi in semiarid region of Brazil. de Souza Lima E, de Oliveira Filho RB, Malta KC, Pires IC, Rosa DS, Chideroli RT, Aburjaile FF, de Jesus Sousa T, Ramos RTJ, Góes-Neto A....Corynebacterium pseudotuberculosis is a bacterium that causes significant economic damage to farmers, mainly in the semiarid region of Northeastern Brazil. In this context, only biovar ovis has been reported, and it is widespread in small ruminants. However, this report describes the first equine case in Brazil attributable to a biovar equi. Clinically, it is the causative agent of ulcerative lymphangitis, mastitis, necrotic and ulcerative dermatitis. A Quarter Horse from the semiarid Northeast presented ulcerative lymphangitis. Culture, nitrate reduction test, MALDI-TOF, and whole-genome sequ...
Novel lineages of bacteria with reduced genomes from the gut of farm animals.
mSphere    June 30, 2025   Volume 10, Issue 7 e0029425 doi: 10.1128/msphere.00294-25
Begmatov S, Beletsky AV, Mardanov AV, Lukina AP, Glukhova LB, Karnachuk OV, Ravin NV.Genome reduction and associated metabolic deficiencies have been described in various lineages of parasitic and symbiotic microorganisms that obtain essential nutrients from their partners, and in some free-living microorganisms that inhabit stable environments. The animal gut is a relatively stable ecosystem, characterized by an abundance of organic substances and a high concentration of microorganisms, which provides favorable conditions for the survival of microorganisms with reduced genomes. Metagenomic analysis of 49 samples of feces of farm animals (cows, sheep, yaks, and horses) reveale...
Rabies in livestock in the United States (2012-2021).
American journal of veterinary research    June 30, 2025   1-8 doi: 10.2460/ajvr.25.01.0019
Payette-Stroman A, Ross Y, Ma X, Boutelle C, Holschbach C, Blanton JD, Wallace R.Rabies in livestock in the United States (US) poses a significant public health concern due to the potential for human exposure and economic losses to producers. Understanding the epidemiology of rabies in livestock supports broader One Health goals by enhancing early detection of viral incursions and protecting both animal and human health. This study assessed the epidemiology of rabies in livestock in the US from 2012 to 2021 using surveillance data reported to the National Rabies Surveillance System. A sensitivity analysis was conducted to estimate economic losses, including livestock value...
Risk Factors Associated with the Prevalence of Antibodies Against Brucellosis in Equids from Western Pará, Brazil.
Microorganisms    June 30, 2025   Volume 13, Issue 7 1525 doi: 10.3390/microorganisms13071525
Picanço EMB, Assis FFV, Peleja PL, Sousa ABB, Barrêto Júnior RA, Lima RF, Neves KAL, Minervino AHH.This study aimed to determine the prevalence of antibodies against equine brucellosis in three distinct equid groups (farm animal, urban carthorse, and sport horse) in Western Pará, Brazil, and to identify associated risk factors. A cross-sectional study, involving 75 farms across 14 municipalities, sampled 1069 equids composed of urban carthorses and sport horses. Serum samples were tested for antibodies against spp. using the buffered acidified plate antigen (BAPA) test for screening, followed by the serum agglutination in tubes with 2-mercaptoethanol (2-ME) for confirmation. Univariate an...
Meat Production Potential of Local Horse Breeds: Sustainable Conservation Through Valorization.
Animals : an open access journal from MDPI    June 28, 2025   Volume 15, Issue 13 1911 doi: 10.3390/ani15131911
Ivanković A, Pećina M, Bittante G, Amalfitano N, Konjačić M, Kelava Ugarković N.Local horse breeds, particularly cold-blood types, are often marginalized in economic and social contexts, primarily due to the neglect of their economic, genetic, and cultural potential, as well as their role in preserving the identity of rural areas, local communities, and ecosystems. The valorization of these breeds is a crucial prerequisite for their economic repositioning. The Croatian Posavina horse is a local breed, well adapted to harsh, extensive production systems. Its sustainability is achieved through pasture-based meat production, primarily targeting foreign European markets. Ensu...
The Effects of the Lactation Period, Mare Age, and Foaling on the Chemical and Physical Composition of Milk from Kazakh Mares Kept Under Natural Pasture Conditions.
Animals : an open access journal from MDPI    June 19, 2025   Volume 15, Issue 12 1817 doi: 10.3390/ani15121817
Toishimanov M, Zhanten O, Kanat R, Beishova I, Ulyanov V, Assanbayev T, Sharapatov T, Daurov D, Daurova A, Sapakhova Z, Nametov A, Shamekova M.This study aimed to evaluate the chemical composition of milk from Kazakh mares kept under free-grazing conditions on natural pasture without supplementary feeding. Fifty Kazakh mares were aged 3 to 13 years, kept in herds, and categorized by age, number of foalings, and lactation stage for statistical analysis. Milk samples were collected, chilled, and analyzed on the same day using a Milkoscan FT2. The composition of fat, protein, lactose, total solids, casein, and other components was examined. Statistical analyses, including ANOVA, PCA, CDA, and Pearson correlation analysis, were performed...
Space-time clustering of rabies in equines in Brazil from 2006 to 2023.
Research in veterinary science    May 27, 2025   Volume 193 105724 doi: 10.1016/j.rvsc.2025.105724
Silva VVD, Leite DPSBM, Gonçalves LMT, Pinto GOA, Oliveira PRF, Mota RA.Rabies is a lethal zoonosis caused by the rabies virus (RABV), primarily transmitted by the common vampire bat (Desmodus rotundus). Brazil, home to the largest equine population in South America, faces ongoing challenges with equine rabies, which impacts both the economy and public health. This study aimed to identify spatial and temporal patterns of rabies in equines in Brazil between 2006 and 2023. Data from the Zoosanitary Information System (SIZ) of the Brazilian Ministry of Agriculture, Livestock, and Food Supply (MAPA) were used. The incidence risk (IR) was calculated for each region and...
Prevalence of trypanosomiasis in domesticated animals in Indonesia: A systematic review and meta-analysis.
Veterinary world    May 25, 2025   Volume 18, Issue 5 1333-1344 
Firdausy LW, Fikri F, Wicaksono AP, Çalışkan H, Purnama MTE.Trypanosomiasis is a vector-borne parasitic disease with significant implications for animal health and rural livelihoods in Indonesia. Despite surveillance efforts, comprehensive national-level estimates of its prevalence in domesticated animals remain lacking. This study aimed to synthesize the pooled prevalence of trypanosomiasis across Indonesian provinces, identify contributing factors, and assess trends over time using a systematic review and meta-analysis. Unassigned: A systematic search was conducted in seven electronic databases (PubMed, Scopus, Web of Science, ScienceDirect, Cochrane...
Targeted pretreatment and inoculation strategies for horse manure fermentation: Impact on metabolites and microbial community composition.
Journal of environmental management    May 21, 2025   Volume 387 125894 doi: 10.1016/j.jenvman.2025.125894
Pipereau K, Trably E, Santa-Catalina G, García-Bernet D, Carrere H.Horse manure is a lignocellulosic biomass found in significant quantities with a vast indigenous flora, not yet fully valorized apart from anaerobic digestion. Its use in the fermentation process can lead to the production of higher-value metabolites. This study investigates three inoculation strategies coupled with five pretreatment conditions for horse manure fermentation. Two microwave pretreatments (200W and 1000W) were compared with a conventional thermal pretreatment, a thermo-acid pretreatment, and an unpretreated condition. The sole horse manure indigenous microorganisms were used in f...
Analysis of Population Structure in Hungarian Coldblood Horses Based on Pedigree Information.
Animals : an open access journal from MDPI    May 13, 2025   Volume 15, Issue 10 1406 doi: 10.3390/ani15101406
Barsi B, Oláh J, Posta J.The Hungarian Coldblood horse was developed in the 1920s by crossing local mares with draft horses imported from Belgium and France, and was approved as an official horse breed in 1954. The aim of the study was to analyze the quality of the pedigree, generation interval, gene origin, and inbreeding. The pedigree information was received from the Hungarian Coldblood Horse Breeding Association. The studbook data of the registered animals up to 2023 were evaluated. Two reference populations were chosen: horses having offspring in 1989 and 2023. The final database contained 21,699 horses. Pedigree...
Laboratory Diagnosis of Animal Tuberculosis in Tracing Interspecies Transmission of Mycobacterium bovis.
Pathogens (Basel, Switzerland)    May 7, 2025   Volume 14, Issue 5 459 doi: 10.3390/pathogens14050459
Szacawa E, Kozieł N, Brzezińska S, Augustynowicz-Kopeć E, Weiner M, Szulowski K, Krajewska-Wędzina M. is one of the most dangerous pathogens of both animals and humans. Bovine tuberculosis (BTB) is a disease caused by mycobacteria belonging to the complex (MTBC), which spreads mainly among domestic cattle but also to mammals other than cattle. The transmission of MTBC between different species requires research and epidemiological investigations to control its spread. When multiple species are a reservoir of infection, it poses a significant public health and veterinary concern. In this study, the diagnosis of alpaca, cattle, horses, dogs, a sheep and a cat from one farm suspected of bovine ...
3000 yr-old patterns of mobile pastoralism revealed by multiple isotopes and radiocarbon dating of ancient horses from the Mongolian Altai.
PloS one    May 7, 2025   Volume 20, Issue 5 e0322431 doi: 10.1371/journal.pone.0322431
Zazzo A, Le Corre M, Lazzerini N, Marchina C, Bayarkhuu N, Bernard V, Cervel M, Fiorillo D, Joly D, Lemoine M, Telouk P, Thil F, Turbat T, Balter V....Pastoral nomadism is of great cultural and economic importance in several regions of the world today. However, documenting ancient patterns of mobility in societies where pastoralism was central is challenging and requires tailored approaches and methodologies. Here we use strontium, oxygen and carbon isotopic analyses of dental enamel, together with a local strontium isoscape, to reconstruct the mobility patterns of seven domestic horses deposited in a Late Bronze Age grave from western Mongolia. Radiocarbon indicates that the animals were deposited within a short period of time, 3000 years a...
Surrogate analyte approach for the determination of endogenous cortisol, cortisone, DHEA, DHEAS in horse hair and sheep wool by LC-HRMS/MS.
Scientific reports    April 29, 2025   Volume 15, Issue 1 14987 doi: 10.1038/s41598-025-99470-2
Two new quantitative methods for the determination of four stress-related hormones (cortisol, cortisone, dehydroepiandrosterone and dehydroepiandrosterone sulfate) in horse hair and sheep wool by liquid chromatography coupled with hybrid high resolution mass spectrometry were developed and validated. Hormones were extracted overnight from ground samples with methanol after a washing step with the same solvent to remove external contamination. The extract was evaporated and dry residue was solubilized in an acid mixture of methanol and water. The methods were validated according to ICH guidelin...
The Industrial Residue of Andiroba (Carapa sp.): A Promising Source of Natural Acaricides Against Dermacentor nitens (Acari: Ixodidae).
Veterinary sciences    April 29, 2025   Volume 12, Issue 5 421 doi: 10.3390/vetsci12050421
Bianchi D, Santos RRD, Peleja PL, de Sousa ABB, da Silva Evangelista M, de Almeida Júnior JS, Barata LES, Moraes WP, Minervino AHH. (tropical horse tick) is a tick species commonly found parasitizing horses and other equids in the Amazon region, causing economic losses. Currently, tick control is based on chemical acaricides, usually in a power formulation for topical use. However, its indiscriminate use results in the emergence of resistant tick lineages. Several plant compounds have been tested for their acaricide activity. In the Amazon, several plants are used for oil extraction, resulting in the production of solid residue (waste) that may contain chemical molecules with biological activities. Thus, we aimed to test ...
Measurement of eco-efficiency in the horse industry, spatiotemporal evolution and convergence analysis.
Scientific reports    April 27, 2025   Volume 15, Issue 1 14729 doi: 10.1038/s41598-025-99073-x
Zhang X, Abdusuli N.The horse industry constitutes a vital economic sector in Xinjiang, China. This study quantitatively assesses the sector's sustainable development through eco-efficiency analysis across northern Xinjiang counties from 2001 to 2021. The research employs four analytical methods: the S-SBM model for efficiency measurement, kernel density estimation for distribution analysis, Moran's index for spatial autocorrelation examination, and convergence tests for long-term trend assessment. Results demonstrate a consistent decline in eco-efficiency, decreasing from 0.821 in 2001 to 0.444 in 2021, with an ...
Comparative digestibility and gas production kinetics of Tifton 85 hay in donkeys and horses.
Journal of equine veterinary science    April 26, 2025   Volume 149 105575 doi: 10.1016/j.jevs.2025.105575
de Morais CBR, Santiago JM, da Silva AH, Nascimento DBD, de Farias IM, da Silva Dias W, Taran FMP, Lucena JEC.This study aimed to verify whether donkeys of the Nordestino ecotype are more efficient than horses concerning apparent digestibility and gas production of Tifton 85 hay at different maturity stages, using the total feces collection method and the in vitro gas production technique. Four donkeys and four horses were used in a 2 × 2 factorial completely randomized design with two Equidae species and hay at two maturity stages (higher quality "Hay 1″ and lower quality "Hay 2″). No difference (P < 0,05) was found between the Equidae species for apparent nutrient digestibility of the Hay 1....
What moves large grazers? Habitat preferences and complementing niches of large herbivores in a Danish trophic rewilding area.
Environmental management    April 25, 2025   Volume 75, Issue 7 1665-1679 doi: 10.1007/s00267-025-02164-8
Rech BJ, Buitenwerf R, Ruggiero R, Trepel J, Waltert M, Svenning JC.Large herbivores (≥45 kg) fulfill key ecological functions. Since the Late Pleistocene megafauna diversity and abundances have declined sharply, with profound consequences for ecosystems. On this background the concept of trophic rewilding has emerged and is increasingly applied to restore natural disturbance regimes and trophic interactions, ultimately aiming to recreate self-sustaining, dynamic and diverse ecosystems. Effects of such efforts (e.g., more heterogeneous habitats) are evident, but herbivore space use, and the resulting distribution of effects on vegetation remain poorly unde...
Sorghum poisoning in ruminants and horses: a review.
Toxicon : official journal of the International Society on Toxinology    April 24, 2025   Volume 261 108375 doi: 10.1016/j.toxicon.2025.108375
Vasconcelos de Sousa AL, Riet-Correa F, Botelho de Castro M, Machado M.We reviewed the history, epidemiology, clinical signs, pathology, pathogenesis, treatment, control, and prophylaxis of Sorghum poisoning in livestock. Economic losses in the livestock industry associated with sorghum have been reported since the 19th century. Hyperacute/acute poisoning associated with cyanide (HCN) or nitrate/nitrite frequently occurs in ruminants that consume high quantities of growth and regrowth sorghum after drought, followed by rainfall, respectively. Chronic cystitis-ataxia syndrome primarily affects horses after weeks of grazing on sorghum pastures, while congenital art...
Pilot Study: Simultaneous Daily Recording of Total Locomotor Activity and Heart Rate in Horses for Application in Precision Livestock Farming.
Animals : an open access journal from MDPI    April 22, 2025   Volume 15, Issue 9 1189 doi: 10.3390/ani15091189
Aragona F, Rizzo M, Arrigo F, Arfuso F, Fazio F, Giudice E, Pugliatti P, Piccione G, Giannetto C.Among physiological parameters, total locomotor activity (TLA) and heart rate (HR) are used as physiological indicators in animal welfare evaluations. The present study aimed to simultaneously record for 24 h the TLA and HR of ten clinically healthy horses housed in conventional individual boxes subjected to a natural photoperiod and temperature. An actigraphy-based data logger was placed on the headstall, and an equine HR monitor was placed around the chest to monitor TLA and HR, respectively. Activity was monitored with 5 min sampling intervals and HR with 5 s intervals. To make the data poi...
Sodium Retention in Large Herbivores: Physiological Insights and Zoogeochemical Consequences.
Journal of experimental zoology. Part A, Ecological and integrative physiology    April 17, 2025   Volume 343, Issue 6 664-676 doi: 10.1002/jez.2924
Abraham AJ, Duvall ES, Doughty CE, Riond B, Ortmann S, Terranova M, le Roux E, Clauss M.The assimilation, retention, and release of nutrients by animals fundamentally shapes their physiology and contributions to ecological processes (e.g., zoogeochemistry). Yet, information on the transit of nutrients through the bodies of large mammals remains scarce. Here, we examined how sodium (Na), a key element for animal health and ecosystem functioning, travels differently through fecal and urinary systems of cows (Bos taurus) and horses (Equus ferus caballus). We provided a large dose of Na and compared its timing of release in feces and urine to that of nonabsorbable markers. Na excreti...
[New drugs for horses and production animals in 2024].
Tierarztliche Praxis. Ausgabe G, Grosstiere/Nutztiere    April 15, 2025   Volume 53, Issue 2 120-131 doi: 10.1055/a-2559-5769
Kamutzki CJ, Emmerich IU.In 2024, there was no release of a novel pharmaceutical agent for horses and food-producing animals or an extension of existing active ingredients to other animal species on the German veterinary medicinal products market. Formic acid is now available for a new form of treatment, the short-term treatment of honeybees, and clenbuterol is available in a new dosage form, as an oral solution for horses. In the field of immunological veterinary medicinal products, three new vaccines were launched on the German veterinary medicinal products market. For the first time, a vaccine against in cattle, a...
Assessing Cadmium Levels in Horses Imported from the European Union and Slaughtered in Italy.
Veterinaria italiana    April 14, 2025   Volume 61, Issue 2 doi: 10.12834/VetIt.3692.31893.2
Dimuccio MM, Ceci E, Bonerba E, Celentano FE, De Marzo P, Leone R, Sportelli S, Bozzo G.The aim of this study was to evaluate cadmium (Cd) concentrations in horses imported from various European Union countries and slaughtered in Italy. In addition, we assessed the potential correlation between Cd levels in tissues and organs, and Cd-related gross and histological lesions. The animals were divided into six groups based on age and geographical origin. Among the 430 animals examined, gross renal lesions were observed in only two kidney samples from Polish horses, which also had the highest Cd concentrations: 9.1 mg/kg w/w and 8.23 mg/kg w/w, respectively. Further histological a...
Prevalence and risk factors of Babesia species in domestic herbivores in China: A systematic review and meta-analysis.
Acta tropica    April 9, 2025   Volume 265 107614 doi: 10.1016/j.actatropica.2025.107614
Wei W, Lan Z, Li C, Liu X, Zhang X, Wang J, Bai X, Zhou L, Wang X, Wang C, Gao J.Babesia species are tick-transmitted hemoprotozoa that infect mammals, birds, and humans, posing a significant global threat to veterinary medicine threat. In this study, the authors systematically retrieved articles on the occurrence of Babesia species in domestic herbivores in China, including ovine, bovine, and equine species, through a search in the following six databases from their inception to 4 November 2024: PubMed, Web of Science, ScienceDirect, Chinese National Knowledge Infrastructure, Wanfang Data, and VIP Chinese Journal Database. A total of 94 studies (54 high-quality studies, 4...
Study of epidemiological and molecular characteristics of Brucella strains circulating in Kazakhstan.
Veterinary research communications    April 1, 2025   Volume 49, Issue 3 156 doi: 10.1007/s11259-025-10725-9
Daugaliyeva A, Daugaliyeva S, Abutalip A, Adambayeva A, Kydyr N, Peletto S.Brucellosis is one of the most common zoonotic infectious diseases of animals. The causative agent of brucellosis is a highly conserved bacterium of the genus Brucella, which includes 14 species with minor genetic changes. Our work aimed to study the epidemiological and molecular characteristics of Brucella strains circulating in Kazakhstan, including the study of polymorphisms in critical virulence genes using single nucleotide polymorphism (SNP) analysis based on whole genome sequencing (WGS). A total of 21 Brucella isolates obtained in 2023-2024 from cattle, sheep and goats, horses, camels,...
Association between sperm DNA fragmentation and fertility parameters in farm animals: a systematic review and meta-analysis.
BMC veterinary research    March 26, 2025   Volume 21, Issue 1 204 doi: 10.1186/s12917-025-04652-9
Abah KO, Ligocka-Kowalczyk Z, Itodo JI, Ameh G, Partyka A, Nizanski W.Accurately predicting male fertility is crucial for the animal breeding industry due to its significant economic implications. Existing literature suggests that mammalian fertility is partially dependent on sperm DNA integrity. However, routine semen analysis often fails to detect DNA damage and does not consistently correlate with field fertility outcomes. While assessing sperm DNA integrity provides valuable biological insights, its role in diagnosing animal infertility remains uncertain. Results: This meta-analysis evaluated the association between sperm DNA fragmentation (SDF) and fertilit...
Working equines: Assessment of welfare and management practices in and around Debre Markos District, Northwest Ethiopia.
Equine veterinary journal    March 25, 2025   doi: 10.1111/evj.14497
Bihon A, Amognehegn D, Derbew G, Yirsa T.Working equines play a crucial role in human livelihoods globally, yet their management is often inadequate. Objective: To assess the welfare and management practices of working equines. Methods: A cross-sectional study was conducted from December 2022 to April 2023 in and around Debre Markos, Northwest Ethiopia. Methods: Physical examination and structured interviews were used as data collection tools. A total of 384 equines (186 donkeys, 144 horses, and 54 mules) and their owners were selected through a purposive sampling method. Results: The results showed that 60.4% of the equines were use...