Analyze Diet

Topic:Nutrition

Nutrition in horses encompasses the study of dietary requirements and feeding practices that support equine health, growth, and performance. It involves the analysis of nutrients such as carbohydrates, proteins, fats, vitamins, and minerals, and their roles in equine physiology. Proper nutrition is essential for maintaining optimal body condition, supporting metabolic processes, and preventing dietary-related disorders. Research in this field examines the nutritional needs of horses at different life stages and activity levels, as well as the effects of various feed types and supplements. This page compiles peer-reviewed research studies and scholarly articles that explore the composition, digestion, and impact of different dietary components on equine health and performance.
Taste Preferences of Horses in Relation to Their Breed and Sex.
Journal of equine veterinary science    February 20, 2018   Volume 64 59-64 doi: 10.1016/j.jevs.2018.02.010
Janczarek I, Wilk I, Pietrzak S, Liss M, Tkaczyk S.The aim of this study was to determine the taste preferences of horses in relation to their breed and sex. Forty-eight adult purebred Arabian, Anglo-Arabian, Polish Konik, and Polish cold-blooded horses-stallions and mares in equal numbers-were examined in the study. During the experiment, five types of industrially processed feeds based on oats and natural dried products commonly regarded as horse treats (i.e., sour and sweet apples, carrots, sugar beet molasses, and barley with salt) were given to horses in buckets on three consecutive days. The behavior of the horses during the experiment ...
Effects of Feeding Frequency Using a Commercial Automated Feeding Device on Gastric Ulceration in Exercised Quarter Horses.
Journal of equine veterinary science    February 16, 2018   Volume 64 96-100 doi: 10.1016/j.jevs.2018.02.005
Bass L, Swain E, Santos H, Hess T, Black J.The objective of this study was to evaluate two feeding regimens: traditional grain feeding (twice a day, traditional fed [TF]) compared with horses fed with an automated feeder programmed to deliver grain in 20 equal aliquots (fractioned fed [FF]) and its effects on gastric ulceration, body weight, and body condition score (BCS). Thirty-one Quarter Horses were maintained in individual stalls during the 60-day experimental period and worked at moderate exercise intensity. Gastroscopies were performed before grain adaptation at 0, 30, and 60 days. Weight and BCS were evaluated biweekly. There ...
Diagnosis and Treatment of Undifferentiated and Infectious Acute Diarrhea in the Adult Horse.
The Veterinary clinics of North America. Equine practice    February 14, 2018   Volume 34, Issue 1 39-53 doi: 10.1016/j.cveq.2017.11.002
Shaw SD, Stämpfli H.Acute, infectious, diarrhea in adult horses is a major cause of morbidity and is associated with numerous complications. Common causes include salmonellosis, clostridiosis, Coronavirus, and infection with Neorickettsia risticii (Potomac horse fever). Treatment is empirical and supportive until results of specific diagnostic tests are available. Supportive care is aimed at restoring hydration, correcting electrolyte imbalances, and limiting the systemic inflammatory response. The mainstays of therapy are intravenous fluid therapy, electrolyte supplementation where necessary, nonsteroidal anti-i...
Enteral/Parenteral Nutrition in Foals and Adult Horses Practical Guidelines for the Practitioner.
The Veterinary clinics of North America. Equine practice    February 14, 2018   Volume 34, Issue 1 169-180 doi: 10.1016/j.cveq.2017.11.012
Carr EA.Nutritional support is an important adjunct to medical therapy in the sick, injured, or debilitated equine patient. What is not clear is the optimal route, composition, or amounts of support. The enteral route should be chosen whenever possible to maximize the benefits to the gastrointestinal tract and the patient as a whole. Complete or partial parenteral nutrition is most useful as a bridge during recovery and transition to enteral feeding in the horse. The reader is encouraged to consider nutritional support whether enteral or parenteral in any anorexic, chronically debilitated, or sick equ...
Submaximal exercise training, more than dietary selenium supplementation, improves antioxidant status and ameliorates exercise-induced oxidative damage to skeletal muscle in young equine athletes.
Journal of animal science    February 13, 2018   Volume 95, Issue 2 657-670 doi: 10.2527/jas.2016.1130
White SH, Warren LK.Exercise is associated with increased production of reactive oxygen species (ROS) as metabolism is upregulated to fuel muscle activity. If antioxidant systems become overwhelmed, ROS can negatively affect health and performance. Adaptation to exercise through regular training has been shown to improve defense against oxidative insult. Given selenium's role as an antioxidant, we hypothesized that increased Se intake would further enhance skeletal muscle adaptations to training. Quarter Horse yearlings (18 ± 0.2 mo; 402 ± 10 kg) were randomly assigned to receive either 0.1 or 0.3 mg Se/kg DM a...
Equine Caregiver Information-Seeking Preferences: Surveys in the Midwest.
Journal of equine veterinary science    February 12, 2018   Volume 64 65-68 doi: 10.1016/j.jevs.2018.02.006
Carroll HK, Bott-Knutson RC, Mastellar SL.Two surveys of equine owners/managers and professionals using convenience sampling via multimodal distribution were conducted on perceptions of equid health and well-being (n = 142) and equine nutrition and feeding practices (n = 151). Surveys were distributed in 2014-2015 (health and well-being) and 2016 (nutrition and feeding) to similar email lists and social media sites; both included questions regarding information-seeking preferences. Respondents were mostly female (62% health and well-being, 84% nutrition and feeding) and had over 20 years of equine ownership/management experience (4...
Horse Husbandry and Preventive Health Practices in Australia: An Online Survey of Horse Guardians.
Journal of applied animal welfare science : JAAWS    February 8, 2018   Volume 21, Issue 4 347-361 doi: 10.1080/10888705.2018.1428099
Thompson KR, Clarkson L, Riley CB, van den Berg M.Little is known about the horse health management practices of Australian horse caregivers (owners). This article presents findings from a convenience sample of 505 horse owners who participated in an online survey. No large-scale welfare issues were identified, but there were some areas of potential concern, including owners who did not regularly deworm their horses (4%), a lack of strategic parasite control (3.1%), and a lack of regular dental care (11%). Several participants did not have their horse's hooves regularly shod or trimmed (2%), and 14% had an unqualified person maintain their ho...
Probiotic Use in Equine Gastrointestinal Disease.
The Veterinary clinics of North America. Equine practice    February 7, 2018   Volume 34, Issue 1 13-24 doi: 10.1016/j.cveq.2017.11.004
Schoster A.Probiotics are commonly used in human and veterinary medicine due to their postulated positive effects on overall and specifically gastrointestinal health. Although some beneficial effects have been shown in several human diseases, a general beneficial effect of probiotics is currently not supported. In horses, well-designed studies to date are few, results are conflicting, and the effects of probiotics are questionable. Adverse effects are rare; however, intestinal adverse effects (diarrhea) have been reported in foals. Quality control of over-the-counter probiotics is not tightly regulated, ...
The contribution of total and free iodothyronines to welfare maintenance and management stress coping in Ruminants and Equines: Physiological ranges and reference values.
Research in veterinary science    February 2, 2018   Volume 118 134-143 doi: 10.1016/j.rvsc.2018.01.025
Ferlazzo A, Cravana C, Fazio E, Medica P.In order to acquire a pattern of thyroid involvement in welfare maintenance in Ruminants and Equines, this review summarizes data concerning the reference values of total and free iodothyronines and their modifications in physiological conditions and in different management conditions (pregnancy, lactation, weaning, growth, isolation, restraint, shearing, confinement and transportation). Thyroidal and extrathyroidal tissues efficiently respond to management practices, giving a differentiated contribution to circulating iodothyronine changes. The hormonal response could be mainly attributed to ...
Evaluation of conjugated linoleic acid supplementation on markers of joint inflammation and cartilage metabolism in young horses challenged with lipopolysaccharide.
Journal of animal science    February 1, 2018   Volume 96, Issue 2 579-590 doi: 10.1093/jas/skx076
Bradbery AN, Coverdale JA, Vernon KL, Leatherwood JL, Arnold CE, Dabareiner RA, Kahn MK, Millican AA, Welsh TH.Seventeen yearling Quarter Horses were used in a randomized complete block design for a 56-d trial to determine ability of dietary CLA to mitigate joint inflammation and alter cartilage turnover following an inflammatory insult. Horses were blocked by age, sex, and BW, and randomly assigned to dietary treatments consisting of commercial concentrate offered at 1% BW (as-fed) supplemented with either 1% soybean oil (CON; n = 6), 0.5% soybean oil and 0.5% CLA (LOW; n = 5; 55% purity; Lutalin, BASF Corp., Florham Park, NJ), or 1% CLA (HIGH; n = 6) top-dressed daily. Horses were fed individually ev...
Evaluation of conjugated linoleic acid supplementation on markers of joint inflammation and cartilage metabolism in young horses challenged with lipopolysaccharide.
Journal of animal science    February 1, 2018   Volume 96, Issue 2 579-590 doi: 10.1093/jas/skx076
Bradbery AN, Coverdale JA, Vernon KL, Leatherwood JL, Arnold CE, Dabareiner RA, Kahn MK, Millican AA, Welsh TH.Seventeen yearling Quarter Horses were used in a randomized complete block design for a 56-d trial to determine ability of dietary CLA to mitigate joint inflammation and alter cartilage turnover following an inflammatory insult. Horses were blocked by age, sex, and BW, and randomly assigned to dietary treatments consisting of commercial concentrate offered at 1% BW (as-fed) supplemented with either 1% soybean oil (CON; n = 6), 0.5% soybean oil and 0.5% CLA (LOW; n = 5; 55% purity; Lutalin, BASF Corp., Florham Park, NJ), or 1% CLA (HIGH; n = 6) top-dressed daily. Horses were fed individually ev...
Advanced nutritional and stem cells approaches to prevent equine metabolic syndrome.
Research in veterinary science    January 31, 2018   Volume 118 115-125 doi: 10.1016/j.rvsc.2018.01.015
Marycz K, Michalak I, Kornicka K.Horses metabolic disorders have become an important problem of modern veterinary medicine. Pathological obesity, insulin resistance and predisposition toward laminitis are associated with Equine Metabolic Syndrome (EMS). Based on pathogenesis of EMS, dietary and cell therapy management may significantly reduce development of this disorder. Special attention has been paid to the diet supplementation with highly bioavailable minerals and mesenchymal stem cells (MSC) which increase insulin sensitivity. In nutrition, there is a great interests in natural algae enriched via biosorption process with...
Equine dietary supplements: an insight into their use and perceptions in the Irish equine industry.
Irish veterinary journal    January 30, 2018   Volume 71 4 doi: 10.1186/s13620-018-0115-3
Murray JMD, Hanna E, Hastie P.Nutritional supplements are frequently used by horse owners/caregivers to supplement their horse(s) diets. Some work has been done to identify the types of supplements fed and the reasons for doing so; however, this has been predominantly disciple-specific and with little focus on participants' perceptions of supplement testing and regulation. The aim of this study was to gain an insight into the use and perceptions of equine dietary supplements in the Irish equestrian industry. Methods: An online survey was designed to ascertain the following information: demographics, types of supplements fe...
Interaction between the sequence of feeding of hay and concentrate, and boiling of barley on feed intake, the activity of hydrolytic enzymes and fermentation in the hindgut of Arabian mares.
Journal of animal physiology and animal nutrition    January 24, 2018   Volume 102, Issue 3 810-817 doi: 10.1111/jpn.12872
Kiani A, Hoseini F, Ghorbaninejad P, Azarfar A, Kreuzer M, Azizi A.The interaction between the sequence of feeding of hay and concentrate and the hydrothermal processing of barley in alleviating concentrate effects on intake, and hindgut fermentation in horses was tested. Six Arabian mares (4-10 years of age, 410 ± 35 kg body weight) were used to evaluate the effects of feeding sequence (FS) and type of barley (TB) on intake, and faecal volatile fatty acids (VFA), activities of α-amylase (AA: EC 3.2.1.1), carboxymethyl cellulase (CMCase: EC 3.2.1.4), microcrystalline cellulase (MCCase: EC 3.2.1.91) and general filter paper degrading activity (FPD). Mare...
Road transport and diet affect metabolic response to exercise in horses.
Journal of animal science    January 3, 2018   Volume 95, Issue 11 4869-4879 doi: 10.2527/jas2017.1670
Connysson M, Muhonen S, Jansson A.This study investigated the effects of transport and diet on metabolic response during a subsequent race-like test in Standardbred horses in training fed a forage-only diet and a 50:50 forage:oats diet. Six trained and raced Standardbred trotter mares were used. Two diets, 1 forage-only diet (FONLY) and 1 diet with 50% of DM intake from forage and 50% from oats (FOATS), were fed for two 29-d periods in a crossover design. At Day 21, the horses were subjected to transport for 100 km before and after they performed an exercise test (transport test [TT]). At Day 26, the horses performed a control...
Influence of short-term dietary starch inclusion on the equine cecal microbiome.
Journal of animal science    January 3, 2018   Volume 95, Issue 11 5077-5090 doi: 10.2527/jas2017.1754
Warzecha CM, Coverdale JA, Janecka JE, Leatherwood JL, Pinchak WE, Wickersham TA, McCann JC.The objective of this study was to determine bacterial community profiles of the equine cecum in response to abrupt inclusion of varying levels of dietary starch. Seven cecally cannulated Quarter Horse geldings (497 to 580 kg) were used in a crossover design with two 28-d periods and a 28-d washout between each. Horses were randomly assigned to dietary treatments consisting of a commercial concentrate offered as fed at either 0.6 (low starch [LS]) or 1.2% BW (high starch [HS]) daily that was divided into 2 meals at 12-h intervals. Prior to the start of each period, horses were allowed ad libit...
Estimation of dietary threonine requirement using plasma amino acid concentrations in mature thoroughbreds.
Animal science journal = Nihon chikusan Gakkaiho    December 28, 2017   Volume 89, Issue 3 625-627 doi: 10.1111/asj.12975
Yoshida T, Ohta Y.The experiment was conducted to estimate dietary threonine (Thr) requirement using plasma amino acid concentrations as a criterion in mature thoroughbreds. Four adult thoroughbreds were used, and a 4 × 4 Latin square design was used for four dietary Thr levels. Plasma Thr concentration was constant until 0.41%, and then increased rapidly with increasing dietary Thr levels. The Thr requirement was estimated to be 67% of lysine with plasma Thr concentration at four Thr levels.
Energy expenditure in professional flat jockeys using doubly labelled water during the racing season: Implications for body weight management.
European journal of sport science    December 7, 2017   Volume 18, Issue 2 235-242 doi: 10.1080/17461391.2017.1406996
Wilson G, Lucas D, Hambly C, Speakman JR, Morton JP, Close GL.To formulate individualized dietary strategies for jockeys, it is vital that energy requirements are quantified. We measured total energy expenditure (TEE) over two separate weeks in spring and summer using doubly labelled water in a group of male flat jockeys (n = 8, 36.9 ± 5.7 years, 164 ± 8 cm, 54.6 ± 2.5 kg). Total energy intake (TEI) was self-recorded, as were all riding and structured exercise activity. Mean daily TEE was 10.83 (±2.3) and 10.66 (±1.76) MJ, (p = .61) respectively. Self-reported TEI were 6.03 (±1.7) and 5.37 (±1.1) MJ (p = .40), respectiv...
Ultrasonographic measurements of localized fat accumulation in Shetland pony mares fed a normal v. a high energy diet for 2 years.
Animal : an international journal of animal bioscience    December 4, 2017   Volume 12, Issue 8 1602-1610 doi: 10.1017/S1751731117003251
Siegers EW, de Ruijter-Villani M, van Doorn DA, Stout TAE, Roelfsema E.Health risks associated with obesity are more likely a factor of the localization of fat excess, rather than of elevated BW per se. The aim of this randomized controlled clinical trial was to determine the effect of a long-term high energy diet on BW, fat accumulation and localization. Eight Shetland pony mares, 3 to 7 years old, were randomly divided into a control and a high energy (HE) diet group fed either maintenance or double maintenance energy requirements (200% net energy (NE)) for two consecutive summers, with a low energy diet in the winter in between. Body condition score (BCS) did ...
Skeletal muscle mitochondrial bioenergetics and associations with myostatin genotypes in the Thoroughbred horse.
PloS one    November 30, 2017   Volume 12, Issue 11 e0186247 doi: 10.1371/journal.pone.0186247
Rooney MF, Porter RK, Katz LM, Hill EW.Variation in the myostatin (MSTN) gene has been reported to be associated with race distance, body composition and skeletal muscle fibre composition in the horse. The aim of the present study was to test the hypothesis that MSTN variation influences mitochondrial phenotypes in equine skeletal muscle. Mitochondrial abundance and skeletal muscle fibre types were measured in whole muscle biopsies from the gluteus medius of n = 82 untrained (21 ± 3 months) Thoroughbred horses. Skeletal muscle fibre type proportions were significantly (p < 0.01) different among the three MSTN genotypes and mito...
Determination of lipid profiles in serum of obese ponies before and after weight reduction by using multi-one-dimensional thin-layer chromatography.
Research in veterinary science    November 21, 2017   Volume 117 111-117 doi: 10.1016/j.rvsc.2017.11.013
Auyyuenyong R, Henze A, Ungru J, Schweigert FJ, Raila J, Vervuert I.Obesity is a key component of equine metabolic syndrome, which is highly associated with laminitis. Feed restriction and/or exercise are known to alleviate the detrimental effects of insulin resistance in obese ponies. However, little is known about changes in the serum lipid patterns due to weight reduction and its association with disease outcomes. Therefore, the lipid patterns in the serum of 14 mature ponies before and after a 14-week body weight reduction program (BWRP) were investigated by multi-one-dimensional thin-layer chromatography (MOD-TLC). Additionally, sensitivity to insulin (SI...
The oral glucose test predicts laminitis risk in ponies fed a diet high in nonstructural carbohydrates.
Domestic animal endocrinology    November 16, 2017   Volume 63 1-9 doi: 10.1016/j.domaniend.2017.10.008
Meier AD, de Laat MA, Reiche DB, Pollitt CC, Walsh DM, McGree JM, Sillence MN.The aim of this study was to investigate the relationship between laminitis development in ponies and insulin/glucose concentrations in response to the oral glucose test (OGT) and a dietary challenge high in nonstructural carbohydrates (NSCs). After undergoing an OGT (1 g dextrose/kg BW in feed), 37 ponies with 2-h serum insulin concentrations ranging from 22 to 1,133 μIU/mL were subjected to a diet challenge period (DCP), consuming 12 g NSC/kg BW/d for up to 18 d. Insulin and glucose responses were measured on day 2 of the DCP. Clinical laminitis was diagnosed by blinded experts and confi...
Evaluating the impact of domestication and captivity on the horse gut microbiome.
Scientific reports    November 14, 2017   Volume 7, Issue 1 15497 doi: 10.1038/s41598-017-15375-9
Metcalf JL, Song SJ, Morton JT, Weiss S, Seguin-Orlando A, Joly F, Feh C, Taberlet P, Coissac E, Amir A, Willerslev E, Knight R, McKenzie V, Orlando L.The mammal gut microbiome, which includes host microbes and their respective genes, is now recognized as an essential second genome that provides critical functions to the host. In humans, studies have revealed that lifestyle strongly influences the composition and diversity of the gastrointestinal microbiome. We hypothesized that these trends in humans may be paralleled in mammals subjected to anthropogenic forces such as domestication and captivity, in which diets and natural life histories are often greatly modified. We investigated fecal microbiomes of Przewalski's horse (PH; Equus ferus p...
Biochemical analysis of some serum trace elements in donkeys and horses in Eastern region of Kingdom of Saudi Arabia.
Veterinary world    October 25, 2017   Volume 10, Issue 10 1269-1274 doi: 10.14202/vetworld.2017.1269-1274
Shawaf T, Almathen F, Meligy A, El-Deeb W, Al-Bulushi S.Little is known about the serum levels of trace elements in donkeys and horses in Saudi Arabia. This study aimed to investigate the levels of some trace elements in these two species in the eastern region of Saudi Arabia and to compare the obtained results with the reference values. Methods: Seventeen Arabian horses and twenty eight Hassawi donkeys were randomly selected for this study. All of the studied healthy animals were kept under a uniform feeding protocol without any minerals supplementations. Atomic absorption spectrometer is used to estimate the serum concentrations of selenium (Se),...
A Summary of New Findings on the Biological Effects of Selenium in Selected Animal Species-A Critical Review.
International journal of molecular sciences    October 21, 2017   Volume 18, Issue 10 doi: 10.3390/ijms18102209
Hosnedlova B, Kepinska M, Skalickova S, Fernandez C, Ruttkay-Nedecky B, Malevu TD, Sochor J, Baron M, Melcova M, Zidkova J, Kizek R.Selenium is an essential trace element important for many physiological processes, especially for the functions of immune and reproductive systems, metabolism of thyroid hormones, as well as antioxidant defense. Selenium deficiency is usually manifested by an increased incidence of retention of placenta, metritis, mastitis, aborts, lowering fertility and increased susceptibility to infections. In calves, lambs and kids, the selenium deficiency demonstrates by WMD (white muscle disease), in foals and donkey foals, it is associated with incidence of WMD and yellow fat disease, and in pigs it cau...
Relationship between carcass traits, prime cuts and carcass grading from foals slaughtered at the age of 13 and 26 months and supplemented with standard and linseed-rich feed.
Animal : an international journal of animal bioscience    October 17, 2017   Volume 12, Issue 5 1084-1092 doi: 10.1017/S1751731117002555
Ruiz M, Sarriés MV, Beriain MJ, Crecente S, Domínguez R, Lorenzo JM.In order to improve foal carcass quality, it is necessary in particular to improve the carcass dressing percentage and tissue composition. Thus, it is important to establish relationships between grading systems and these parameters. This research was conducted to study the effect of slaughter age (13 v. 26 months) and finishing feed (standard v. linseed feed) on carcass characteristics such as subcutaneous fat colour plus classification of foals for the degree of fatness and conformation. For this study, 46 foals of crossbred genotype (Galician Mountain×Burguete) were used. Finishing feed di...
Influences of different dietary contents of macrominerals on the availability of trace elements in horses.
Journal of animal physiology and animal nutrition    October 13, 2017   Volume 102, Issue 2 e633-e640 doi: 10.1111/jpn.12805
Neustädter LT, Kamphues J, Ratert C.In this study, influences of a reduced macromineral intake on the trace element metabolism in horses at maintenance were investigated. Background of this study is the revised recommendation on the macromineral supply for horses (GfE ). Balance studies on three adult pony geldings with body weights of 405 / 348 / 384 kg were performed to obtain data on apparent digestibility (aD), retention and serum concentrations of different trace elements (Cu, Zn, Se) at different dietary macromineral levels. A mineral supplement or a complementary feed-with a reduced macromineral content-was added to a ha...
Rapid Communication: Dietary selenium improves skeletal muscle mitochondrial biogenesis in young equine athletes.
Journal of animal science    October 11, 2017   Volume 95, Issue 9 4078-4084 doi: 10.2527/jas2017.1919
White SH, Wohlgemuth S, Li C, Warren LK.Exercise is known to promote mitochondrial biogenesis in skeletal muscle as well as enhance mitochondrial function and efficiency in human and rodent models. These adaptations help to decrease exercise-associated production of reactive oxygen species, which can negatively affect health and performance if antioxidant mechanisms are overwhelmed. Little is known about the adaptations of mitochondria in response to exercise training in the growing horse or if supplementation with a dietary antioxidant can improve mitochondrial function. To evaluate the separate and combined effects of selenium (Se...
Incidence of laminitis and survey of dietary and management practices in pleasure horses and ponies in south-eastern Australia.
Australian veterinary journal    September 28, 2017   Volume 95, Issue 10 370-374 doi: 10.1111/avj.12635
Potter SJ, Bamford NJ, Harris PA, Bailey SR.The aims of this study were to (1) report the incidence of laminitis among a population of horses and ponies attending Pony Clubs in Victoria, Australia, and (2) describe the dietary and management practices of the sample population. Methods: Researchers visited 10 Pony Clubs over a 10-month period. Horse and pony owners completed a questionnaire to provide information on management relating to diet and exercise. Owners were also asked to report their animal's history of laminitis, if any. Results: From a survey population of 233 horses and ponies, 15.0% of animals (35 individuals) were report...
Trace elements in struvite equine enteroliths: Concentration, speciation and influence of diet.
Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS)    September 19, 2017   Volume 45 23-30 doi: 10.1016/j.jtemb.2017.09.019
Rouff AA, Lager GA, Arrue D, Jaynes J.Equine enteroliths ∼1.5cm in diameter were collected from an Arabian horse in Louisville, Kentucky, United States. Scanning electron microscopy (SEM) and light microscope imaging of a sectioned enterolith showed two distinct regions of concentric growth outward from the central nidus, a small pebble. After initial growth, acidic colonic fluids permeated the stone inducing recrystallization and alteration of crystals closest to the nidus. A second growth event, when mineral crystallization was again favorable, produced an outer region of unaltered crystals at the rim. The mineral was identifi...
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