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Identification and genetic characterization of equine infectious anemia virus in Western Balkans.
BMC veterinary research    April 15, 2021   Volume 17, Issue 1 168 doi: 10.1186/s12917-021-02849-2
Equine infectious anemia (EIA) is a viral disease, caused by the Equine Infectious Anemia virus (EIAV) belonging to the Retroviridae family, genus Lentivirus. Horses (or equids) infected with EIAV are lifelong carriers and they remain contagious for other horses even in the absence of clinical signs. So far, EIAV infection has been reported among horses in North and South America, France, Germany, Italy, Hungary and Romania, with no publication regarding the presence of EIAV in horses in Serbia. To determine the circulation of EIAV among, approximately, the 5000 horses of the Vojvodina region,...
Effect of three different needle holders on gastrointestinal anastomosis construction time and bursting pressure in equine jejunal segments.
BMC veterinary research    April 15, 2021   Volume 17, Issue 1 167 doi: 10.1186/s12917-021-02871-4
Averay K, van Galen G, Ward M, Verwilghen D.Equine small intestinal resection and anastomosis is a procedure where optimizing speed, without compromising integrity, is advantageous. There are a range of different needle holders available, but little is published on the impact surgical instrumentation has on surgical technique in veterinary medicine. The objectives of this study were to investigate if the needle holder type influences the anastomosis construction time, the anastomosis bursting pressure and whether the bursting pressure is influenced by the anastomosis construction time. Single layer end-to-end jejunojejunal anastomoses w...
Pedigree Analysis of Warmblood Horses Participating in Competitions for Young Horses.
Frontiers in genetics    April 15, 2021   Volume 12 658403 doi: 10.3389/fgene.2021.658403
Próchniak T, Kasperek K, Knaga S, Rozempolska-Rucińska I, Batkowska J, Drabik K, Ziȩba G.The aim of the study was to characterize the population structure and assess the genetic diversity of warmblood horses used in the show jumping discipline. Pedigree data of 1,048 horses participating in the Polish Championships for Young Horses were analyzed. The pedigree of these animals included 12 863 individuals. The study consisted in analysis of the pedigree structure of the horses and characterization of the homozygosity and genetic diversity in the population. It was found that pedigree completeness and depth were sufficient for reliable assessment of the genetic diversity in the analy...
Comparison of PCR-based methods for the detection of Babesia caballi and Theileria equi in field samples collected in Central Italy.
Parasitology research    April 15, 2021   Volume 120, Issue 6 2157-2164 doi: 10.1007/s00436-021-07153-4
Equine piroplasmosis (EP) is a disease of equids caused by Theileria equi and Babesia caballi, members of the order Piroplasmida, transmitted by several species of ticks. As the disease is endemic in many countries, a clinical examination or a serological test are required prior to movement of horses to prove freedom from infection and to avoid the introduction of EP with its sanitary and economic impact, especially in areas where it is absent. Currently, numerous diagnostic PCR protocols are available, some of which are recommended by the World Organisation for Animal Health (OIE). In order t...
Experiences of Military Veterans in a Therapeutic Horseback Riding Program.
Clinical nursing research    April 15, 2021   Volume 30, Issue 7 923-933 doi: 10.1177/10547738211003580
Johnson RA, Albright DL, Marzolf JR, Bibbo JL, Yaglom HD, Crowder SM, Carlisle GM, Grindler K, Harms N, Willard A, Wassman M, Russell CL.Posttraumatic stress disorder commonly occurs among U.S. military veterans. Therapeutic horseback riding (THR) has emerged as an adjunct therapy. We explored 20 veterans' perceived benefits, drawbacks and views of a 6-week THR program. Participants had confirmed diagnoses of posttraumatic stress disorder, traumatic brain injury, or both. Veterans rode the same horse weekly, the same day, at the same time. Data were collected as part of a randomized clinical trial testing the effects of THR on Post-Traumatic Stress Disorder. Veterans responded to an open-ended questionnaire. Content analysis wa...
Oxidative and local histopathological response on skin wound of horses due to Amblyomma sculptum tick parasitism.
Research in veterinary science    April 15, 2021   Volume 136 550-560 doi: 10.1016/j.rvsc.2021.04.013
Nogueira BCF, Campos AK, Alves RS, de Cássia Vieira Faria R, Sarandy MM, Fonseca E Silva F, Gonçalves RV.Amblyomma sculptum is frequently observed parasitizing horses, responsible for economic losses, damage to the host''s skin and transmission of pathogens. The oxidative stress profile and inflammatory mechanisms involved in this parasitism remain poorly studied. Thus, this study aimed to assess the histopathological changes and oxidative profile responses of horses in the attachment site of A. sculptum to find variations that indicate resistance and susceptibility between the breeds to this tick, based on the hypothesis that resistant animals have a greater inflammatory response and lesser numb...
Images–ECG Localization in ST-Elevation Myocardial Infarction: Spotting the Zebra Among the Horses.
Journal of the Saudi Heart Association    April 15, 2021   Volume 33, Issue 1 51-52 doi: 10.37616/2212-5043.1240
Gujral JS, Shrivastava A.No abstract available
The Physical Activity-Dependent Hematological and Biochemical Changes in School Horses in Comparison to Blood Profiles in Endurance and Race Horses.
Animals : an open access journal from MDPI    April 14, 2021   Volume 11, Issue 4 1128 doi: 10.3390/ani11041128
Maśko M, Domino M, Jasiński T, Witkowska-Piłaszewicz O.Blood testing is one of the most important ways to improve performance, facilitate recovery and monitor the training of endurance and race horses. However, little is known about the physical activity-dependent changes of blood parameters in horses used for pleasure and in riding schools. This study aimed to perform routine blood tests for training monitoring of sport horses in three different horse types of use. Then the values of blood indicators were compared between school, endurance and race horses to find similarities in the physical activity-dependent profile. The study was carried out o...
A QTL for conformation of back and croup influences lateral gait quality in Icelandic horses.
BMC genomics    April 14, 2021   Volume 22, Issue 1 267 doi: 10.1186/s12864-021-07454-z
Rosengren MK, Sigurðardóttir H, Eriksson S, Naboulsi R, Jouni A, Novoa-Bravo M, Albertsdóttir E, Kristjánsson Þ, Rhodin M, Viklund Å, Velie BD....The back plays a vital role in horse locomotion, where the spine functions as a spring during the stride cycle. A complex interaction between the spine and the muscles of the back contribute to locomotion soundness, gait ability, and performance of riding and racehorses. Conformation is commonly used to select horses for breeding and performance in multiple horse breeds, where the back and croup conformation plays a significant role. The conformation of back and croup plays an important role on riding ability in Icelandic horses. However, the genes behind this trait are still unknown. Therefor...
Antigenic differences between equine influenza virus vaccine strains and Florida sublineage clade 1 strains isolated in Europe in 2019.
Veterinary journal (London, England : 1997)    April 14, 2021   Volume 272 105674 doi: 10.1016/j.tvjl.2021.105674
Nemoto M, Ohta M, Yamanaka T, Kambayashi Y, Bannai H, Tsujimura K, Yamayoshi S, Kawaoka Y, Cullinane A.From late 2018 to 2019, equine influenza virus (EIV) strains of Florida sublineage clade 1 (Fc1), which had until then been circulating mainly in the United States, suddenly spread across Europe causing many outbreaks, and Florida sublineage clade 2 (Fc2) strains, which had been circulating mainly in Europe, have not been detected in Europe since 2018. Since 2010, the World Organisation for Animal Health (OIE) has recommended that EIV vaccines contain an Fc1 strain that is like A/equine/South Africa/4/2003 or A/equine/Ohio/2003. Accordingly, Japanese vaccines contain A/equine/Ibaraki/1/2007 as...
Survey of anthelmintic resistance in a Romanian horse stud using three different methods.
Polish journal of veterinary sciences    April 14, 2021   Volume 24, Issue 1 145-149 doi: 10.24425/pjvs.2021.136803
Dărăbuş G, Badea C, Oprescu I, Morariu S, Mederle N, Ilie M, Suici T, Imre M.Resistance of cyathostomins to benzimidazole (BZ) anthelmintics is widespread in horses in many parts of the world. This study compared three methods for the determination of benzimidazole resistance of Cyathostominae in 18 horses from a stud farm in Romania. The horses were treated with Fenbendazole. The resistance test was performed by FECRT, ERP and PCR. On Day 0, larvae of species belonging to the Cyathostominae subfamily, types A, B, C, D and Gyalocephalus, as well as Strongylus vulgaris species of the Strongylinae subfamily, were identified. At 42 days post treatment with fenbendazole on...
Effect of Intrauterine Infusion of Equine Fresh Platelets-Rich Plasma (PRP) or Lyophilized PRP (L-GFequina) on Ovarian Activity and Pregnancy Rate in Repeat Breeder Purebred Arabian Mares.
Animals : an open access journal from MDPI    April 14, 2021   Volume 11, Issue 4 doi: 10.3390/ani11041123
Dawod A, Miro J, Elbaz HT, Fahmy H, Abdoon AS.This study was designed to examine the effect of the intrauterine infusion of platelet-rich plasma (PRP) or equine lyophilized growth factor (L-GFequina) on the follicular growth, endometrial thickness, estrus cycle length, and pregnancy rate in purebred Arabian mares. A total of 73 purebred Arabian mares who experienced repeat breeding for three successive cycles were randomly divided into the following three groups: control group, without treatment; second Group (PRP group), in which mares were intrauterine infused with 20 mL of fresh PRP on the second day after the end of physic estrus phas...
Internal cavity amplification of shell-like ferritin regulated with the change of the secondary and tertiary structure induced by PEF technology.
International journal of biological macromolecules    April 14, 2021   Volume 182 849-857 doi: 10.1016/j.ijbiomac.2021.04.072
Zhang S, Li Y, Bao Z, Sun N, Lin S.In this study, the effect of pulsed electric field (PEF) on apparent morphology and molecular structure of shell-like ferritin obtained from horse spleen was determined by circular dichroic (CD), fluorescence spectroscopy, Raman spectroscopy, cold field emission scanning electron microscopy (CF-SEM) and transmission electron microscopy (TEM), and verified by molecule dynamics (MD) simulation. After PEF treatment, the α-helix content of the samples reached a minimum value at 10 kV/cm, which indicated that the ferritin structure has been partially unfolded. However, the α-helix content peaked ...
Reply to Shelach-Lavi et al.: Implications of the horse assemblages from Shirenzigou and Xigou.
Proceedings of the National Academy of Sciences of the United States of America    April 14, 2021   Volume 118, Issue 16 e2025947118 doi: 10.1073/pnas.2025947118
Li Y, Zhang C, Taylor WTT, Chen L, Flad RK, Boivin N, Liu H, You Y, Wang J, Ren M, Xi T, Han Y, Wen R, Ma J.No abstract available
Simultaneous quantification of vitamin E and vitamin E metabolites in equine plasma and serum using LC-MS/MS. Habib H, Finno CJ, Gennity I, Favro G, Hales E, Puschner B, Moeller BC.Vitamin E deficiencies can impact normal growth and development in humans and animals, and assessment of circulating levels of vitamin E and its metabolites may be an important endpoint for evaluation. Development of a sensitive method to detect and quantify low concentrations of vitamin E and metabolites in biological specimens allows for a proper diagnosis for patients and animals that are deficient. We developed a method to simultaneously extract, detect, and quantify the vitamin E compounds alpha-tocopherol (α-TP), gamma-tocopherol (γ-TP), alpha-tocotrienol (α-TT), and gamma-tocotrienol...
Flexibility of equine bioenergetics and muscle plasticity in response to different types of training: An integrative approach, questioning existing paradigms.
PloS one    April 13, 2021   Volume 16, Issue 4 e0249922 doi: 10.1371/journal.pone.0249922
de Meeûs d'Argenteuil C, Boshuizen B, Oosterlinck M, van de Winkel D, De Spiegelaere W, de Bruijn CM, Goethals K, Vanderperren K, Delesalle CJG.Equine bioenergetics have predominantly been studied focusing on glycogen and fatty acids. Combining omics with conventional techniques allows for an integrative approach to broadly explore and identify important biomolecules. Friesian horses were aquatrained (n = 5) or dry treadmill trained (n = 7) (8 weeks) and monitored for: evolution of muscle diameter in response to aquatraining and dry treadmill training, fiber type composition and fiber cross-sectional area of the M. pectoralis, M. vastus lateralis and M. semitendinosus and untargeted metabolomics of the M. pectoralis and M. vastus late...
Effect of uterine ozone therapy and anticoagulant sampling on oxidative stress parameters in mares.
Research in veterinary science    April 13, 2021   Volume 136 503-511 doi: 10.1016/j.rvsc.2021.04.002
Almeida BFM, Amatti LZ, de Souza GG, Garcia LV, Montechiesi DF, Ignácio FS, de Oliveira PL, Costa LR, Floriano BP, Bosculo MRM, Joaquim JGF, Rubio CP.With the purpose of assessing the effects of uterine ozone therapy and anticoagulant sampling on oxidative stress (OS) parameters in mares, ten mares underwent three consecutive days of uterine ozone therapy by flushing the uterus with ozonated lactated Ringer's solution followed by insufflation with ozone‑oxygen gas. Serum samples were obtained at baseline and days 3, 6, 10 and 17 to determine the effect of ozone therapy on OS markers. Plasma obtained with anticoagulants citrate, ethylenediaminetetraacetic acid (EDTA) and heparin were at baseline and 6 days following therapy to determine t...
A Systematic Approach to Comparing Thermal Activity of the Thoracic Region and Saddle Pressure Distribution beneath the Saddle in a Group of Non-Lame Sports Horses.
Animals : an open access journal from MDPI    April 13, 2021   Volume 11, Issue 4 1105 doi: 10.3390/ani11041105
MacKechnie-Guire R, Fisher M, Mathie H, Kuczynska K, Fairfax V, Fisher D, Pfau T.Thermography is a non-invasive method for measuring surface temperatures and may be a convenient way of identifying hypo/hyperthermic areas under a saddle that may be related to saddle pressures. A thermal camera quantified minimum/maximum/mean temperatures at specific locations (left/right) of the thoracic region at three-time points: (1) baseline; (2) post lunging; (3) post ridden exercise in eight non-lame sports horses ridden by the same rider. A Pliance (Novel) pressure mat determined the mean/peak saddle pressures (kPa) in the cranial and caudal regions. General linear mixed models with ...
Comparison of Mesh and Barbed Suture for Laparoscopic Nephrosplenic Space Ablation in Horses.
Animals : an open access journal from MDPI    April 12, 2021   Volume 11, Issue 4 doi: 10.3390/ani11041096
Gialletti R, Nannarone S, Gandini M, Cerullo A, Bertoletti A, Scilimati N, Giusto G.Nephrosplenic space (NSS) ablation has been demonstrated to be an effective technique for prevention of left dorsal displacement of the large colon and multiple laparoscopic techniques, among which ablation with mesh or with a barbed suture, have been proposed. Our objective was to compare two laparoscopic techniques for closure of the NSS in twenty-eight horses diagnosed with nephrosplenic entrapment. Medical records of horses that had laparoscopic NSS ablation in two referral centers between 2017-2019 were retrieved. Duration of surgery, complications, and short- and long-term follow-up info...
Effects of Aleurone Supplementation on Glucose-Insulin Metabolism and Gut Microbiome in Untrained Healthy Horses.
Frontiers in veterinary science    April 12, 2021   Volume 8 642809 doi: 10.3389/fvets.2021.642809
Boshuizen B, Moreno de Vega CV, De Maré L, de Meeûs C, de Oliveira JE, Hosotani G, Gansemans Y, Deforce D, Van Nieuwerburgh F, Delesalle C.Aleurone, a layer of the bran fraction, is deemed to be responsible for the positive health effects associated with the consumption of whole-grain products. Studies on rodents, pigs, and humans report beneficial effects of aleurone in five main areas: the reduction of oxidative stress, immunomodulatory effects, modulation of energy management, digestive health, and the storage of vitamins and minerals. Our study is the first aleurone supplementation study performed in horses. The aim of this study was to investigate the effect of an increase in the dose levels of aleurone on the postprandial g...
Microvascularization and Expression of Fibroblast Growth Factor and Vascular Endothelial Growth Factor and Their Receptors in the Mare Oviduct.
Animals : an open access journal from MDPI    April 12, 2021   Volume 11, Issue 4 1099 doi: 10.3390/ani11041099
Pinto-Bravo P, Rebordão MR, Amaral A, Fernandes C, Galvão A, Silva E, Pessa-Santos P, Alexandre-Pires G, Roberto da Costa RP, Skarzynski DJ....The oviduct presents the ideal conditions for fertilization and early embryonic development. In this study, (i) vascularization pattern; (ii) microvascular density; (iii) transcripts of angiogenic factors (, , ) and their receptors-, , , respectively, and (iv) the relative protein abundance of those receptors were assessed in cyclic mares' oviducts. The oviductal artery, arterioles and their ramifications, viewed by means of vascular injection-corrosion, differed in the infundibulum, ampulla and isthmus. The isthmus, immunostained with CD31, presented the largest vascular area and the highest ...
A highly prevalent SINE mutation in the myostatin (MSTN) gene promoter is associated with low circulating myostatin concentration in Thoroughbred racehorses.
Scientific reports    April 12, 2021   Volume 11, Issue 1 7916 doi: 10.1038/s41598-021-86783-1
O'Hara V, Cowan A, Riddell D, Massey C, Martin J, Piercy RJ.Horse racing is a popular and financially important industry worldwide and researchers and horse owners are interested in genetic and training influences that maximise athletic performance. An association has been found between the presence of a short interspersed nuclear element (SINE) mutation in the myostatin (MSTN) gene promoter and optimal race distance in Thoroughbred horses. There is previous laboratory evidence that this mutation reduces MSTN expression in a cell culture model and influences skeletal muscle fibre type proportions in horses. Manipulating MSTN expression has been propose...
Prevalence and Molecular Characterization of Coxiella burnetii in Cattle, Goats, and Horses in the Republic of Korea.
Vector borne and zoonotic diseases (Larchmont, N.Y.)    April 12, 2021   Volume 21, Issue 7 502-508 doi: 10.1089/vbz.2020.2764
Cho HC, Hwang S, Kim EM, Park YJ, Shin SU, Jang DH, Chae JS, Choi KS. is an obligate intracellular zoonotic bacterium with a global distribution. This study was conducted to investigate the prevalence of in different animals and to assess the potential role of these species as reservoirs of infection and transmission to humans. A total of 592 blood samples (105 beef cattle, 61 dairy cattle, 110 Korean native goats, 83 Boer goats, and 233 horses) were collected in the Republic of Korea (ROK). The DNA was detected from blood samples using the transposon-like repetitive region () by PCR method. The results showed that 22.7% of the Korean-native goats, 16.4% of t...
Short-term infusion of ultralow-dose dopamine in an adult horse with acute kidney injury: A case report.
Veterinary and animal science    April 11, 2021   Volume 12 100176 doi: 10.1016/j.vas.2021.100176
Matsuda H, Matsuda K, Muko R, Oikawa MA, Tanaka A.Much is known regarding a good prognosis of acute kidney injury (AKI) is achieved with adequate, intensive, and early treatment, which leads to acceleration of the renal blood flow rate and associated urination. Low-dose dopamine (1 to 5 μg/kg bwt per min) is a treatment option for AKI in humans but remains controversial for use in horses because of the lack of extensive clinical trial data. A 19-year-old Westfalen horse gelding was referred to the Animal Medical Center with a 1-hour history of mild abdominal pain and anorexia after dressage exercise for 1 hour. Since elevated serum levels o...
Letter to the Editor: Validation of standing cone beam computed tomography for diagnosing subchondral fetlock pathology in the Thoroughbred racehorse.
Equine veterinary journal    April 10, 2021   Volume 53, Issue 3 628-629 doi: 10.1111/evj.13433
Ramzan PHL.No abstract available
Clinical insights: Equine analgesia.
Equine veterinary journal    April 10, 2021   Volume 53, Issue 3 407-409 doi: 10.1111/evj.13421
Mackenzie C.No abstract available
Science-in-brief: Report on the Global Equine Endocrinology Symposium.
Equine veterinary journal    April 10, 2021   Volume 53, Issue 3 414-416 doi: 10.1111/evj.13405
Bennett M, McGowan CM.No abstract available
Owners urged to take precautions to reduce risk of EHV-1.
The Veterinary record    April 10, 2021   Volume 188, Issue 7 269-270 doi: 10.1002/vetr.374
Rendle D.No abstract available
Equine respiratory viruses, airway inflammation and performance in thoroughbred racehorses.
Veterinary microbiology    April 10, 2021   Volume 257 109070 doi: 10.1016/j.vetmic.2021.109070
Couetil L, Ivester K, Barnum S, Pusterla N.Equine asthma is a common cause of poor performance in racehorses but it is unclear if respiratory viruses contribute to its etiology. The objective of the study was to determine if respiratory viruses were associated with clinical signs and bronchoalveolar lavage fluid (BALF) cytology in Thoroughbred racehorses. Equine herpesviruses (EHV-1, 2, 4, 5) and equine rhinitis A and B viruses (ERBV, ERAV) genomes were quantified by qPCR in nasopharyngeal, tracheal, and BALF samples collected after racing. The relationships between virus detection and load and clinical signs, performance, BALF cytolog...
Response to Letter to the Editor: Validation of standing cone beam computed tomography for diagnosing subchondral fetlock pathology in the Thoroughbred racehorse.
Equine veterinary journal    April 10, 2021   Volume 53, Issue 3 630-631 doi: 10.1111/evj.13441
Wulster K, Ortved K, Curtiss A, Richardson D.No abstract available