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Topic:Ambient Temperature

Ambient temperature is a critical environmental factor affecting the physiology, behavior, and overall well-being of horses. Horses, being homeothermic animals, rely on thermoregulation to maintain a stable internal body temperature despite changes in external conditions. Variations in ambient temperature can influence metabolic rate, hydration status, and performance. Extreme temperatures, whether hot or cold, pose challenges that can lead to heat stress or hypothermia, respectively, impacting equine health and productivity. Understanding how horses adapt to different thermal environments is essential for optimizing management practices, ensuring animal welfare, and improving performance outcomes. This page compiles peer-reviewed research studies and scholarly articles that explore the effects of ambient temperature on equine physiology, the mechanisms of thermoregulation, and strategies for managing horses in varying climatic conditions.
The fertility of a sub-population of stallions is negatively affected by ambient climatic conditions, mediated through DNA damage.
International journal of biometeorology    March 9, 2026   Volume 70, Issue 3 89 doi: 10.1007/s00484-025-03123-y
Griffin RA, Miller K, Colyvas K, Sheridan A, De Iuliis G, Aitken RJ, Baker MA, Gibb Z, Swegen A.Exposure to systemic heat stress in male mammals adversely affects sperm production, fertility, and DNA integrity. To date, few studies have investigated this phenomenon in horses, particularly in industry-relevant environments. Therefore, this study examined the relationship between ambient climatic conditions and fertility within a population of commercially fertile stallions. Post-coital semen samples ( = 804) were collected weekly from 46 Thoroughbred stallions during two successive breeding seasons (NSW, Australia; 22 weeks total). Semen samples were processed via single-layer colloid...
Assessment of thermal imaging to objectively body condition score mature horses and multiparous gestating beef cows.
Translational animal science    September 15, 2025   Volume 9 txaf121 doi: 10.1093/tas/txaf121
Webster AP, Wright RK, Hammond JB, Kotey NA, Gleason CB, White RR.This study explored whether thermal imaging could provide an objective means of body condition scoring (BCS) horses and multiparous, gestating beef cows. This study consisted of two parts: one part assessed BCS in horses of the Quarter Horse or Thoroughbred breed types while the other evaluated BCS of gestating beef cows. Ground truth BCS were assigned by five to eight trained scorers for each animal. Thermal images were also collected from one or both sides of the body and analyzed for surface temperature. Surface temperature and BCS were evaluated with the whole body, and for five (cows) or ...
Temperatures of the Mouthpiece of the Bit of Carriage Horses over a Period of 11 Months.
Animals : an open access journal from MDPI    September 7, 2025   Volume 15, Issue 17 2623 doi: 10.3390/ani15172623
Krcal C, Licka T.Besides oral temperature, meteorological parameters are expected to be relevant for mouthpiece temperature, potentially influenced by the material, surface area and weight of the bit. This study measured the temperature of the mouthpieces at the corner of the mouth while they were in use on 58 carriage horses during the four 2024 seasons. Stainless steel, copper, and copper-steel bits were tested in three shapes: Butterfly Liverpool, Liverpool, and Loose Ring Snaffle with four rings. Additionally, surface temperatures of inner thighs, the ground, and buildings were measured using infrared ther...
Effects of Sedation on Rectal Temperature During Equine Dental Procedures Performed in Cold to Warm Ambient Temperatures.
Journal of veterinary dentistry    September 3, 2025   Volume 43, Issue 2 128-134 doi: 10.1177/08987564251371523
Norman AJ, Turner CM, Manfredi JM.Sedation is important for safe equine dental procedures, but it is unknown if there is a higher risk of sedation causing hypothermia in procedures performed during cold months. The authors hypothesize that sedation for a dental procedure will significantly decrease rectal body temperature in cold as compared to warm ambient temperatures. Adult equids ( = 246), from a private equine dentistry practice, that underwent dental equilibration, were selected for the study. Each horse's weight was estimated and sedation was administered intravenously (0.01 mg/kg detomidine and 0.01 mg/kg butor...
Road transportation is associated with decreased intestinal motility in horses.
Frontiers in veterinary science    August 18, 2025   Volume 12 1647236 doi: 10.3389/fvets.2025.1647236
Raidal SL, Freccero F, Carstens A, Weaver S, Padalino B.Transportation of horses has been associated with colic and changes to the gastrointestinal microbiome. Percutaneous abdominal ultrasonography using wireless, point-of-care transducers can be used to assess gastrointestinal motility in field settings. Unassigned: Characterization of intestinal motility and salivary cortisol responses of horses completing commercial transportation of 10-12h. Unassigned: Prospective observational study of 30 horses with diverse signalment and transport histories. Clinical parameters, sonographic assessment of intestinal motility and saliva samples, were collecte...
The Effect of Ambient Temperature, Relative Humidity, and Temperature-Humidity Index on Stress Hormone and Inflammatory Response in Exercising Adult Standardbred Horses.
Animals : an open access journal from MDPI    May 15, 2025   Volume 15, Issue 10 1436 doi: 10.3390/ani15101436
In the present study, the changes in the markers of stress and inflammatory responses of 12 Standardbred horses were investigated before and after exercise during late spring and summer. The environmental temperature, relative humidity, and temperature-humidity index (THI) were assessed during this study. From horses, blood sampling, measurement of heart and respiratory rate (HR and RR), and measurement of rectal temperature (RT) were performed before exercise (Pre) and within 5 min following the end of the exercise (Post). THI values showed mild stress in June and high stress in July. Direct ...
Performance of a non-contact veterinary infrared thermometer and reference intervals of equine temperature at different body sites.
Frontiers in veterinary science    April 17, 2025   Volume 12 1583839 doi: 10.3389/fvets.2025.1583839
Alberghina D, Tombolani C, Quintavalla F.Evaluating the body temperature of horses () is essential for monitoring their health. Rectal thermometry is the most common method for determining the temperatures of adult horses and foals. However, this method requires restraining the animals for several seconds, and it poses potential risks to both horses and humans. This study compared infrared and rectal thermometry in 126 horses, evaluating age and measurement site influences on body temperature. Horses were divided into three groups: foals (4-12 months), Shetland ponies (8-12 months), and adult horses (2-30 years). Rectal, ocular...
Modeling climate change effects on some biochemical parameters in horse.
Research in veterinary science    March 26, 2025   Volume 189 105630 doi: 10.1016/j.rvsc.2025.105630
Changes in the global climate pose a severe threat to human and animal welfare and productivity. Total proteins (TP), globulins (GLOB), albumins (ALB), alkaline phosphatase (ALP), creatine kinase (CK), lactate dehydrogenase (LDH), aspartate aminotransferase (AST), blood urea nitrogen (BUN), γ-glutamyl transferase (GGT), and creatinine (CREA) were evaluated during a three-year monitoring period (2021-23) on 16 Thoroughbred retired mares from the regional Golkoy Breeding Farm in Kastamonu- Turkey. The following thermal and hygrometric parameters were gathered: ambient temperature (AT), relative...
Short-term effect of weather variables on West Nile virus infection in Equids in Spain: A space-time stratified case-crossover design.
Acta tropica    March 25, 2025   Volume 264 107602 doi: 10.1016/j.actatropica.2025.107602
Fonseca-Rodríguez O, Pailler-García L, Urban A, Cáceres G, Napp S, Busquets N.West Nile virus (WNV) is an arthropod-borne virus primarily transmitted by Culex mosquitoes, among birds, but occasionally infecting humans and equids. Understanding environmental factors on the disease occurrence is crucial for planning effective prevention and control strategies. Our study estimates the short-term effects of ambient temperature, relative humidity and precipitation on weekly cases of WNV in equids in Spain, using a space-time stratified case-crossover design. Because WNV transmission occurs sometime after the weather conditions become favorable for the vector, we aimed to est...
Investigation of the impact of seasonal climate conditions on feed intake and body weight in horses.
International journal of biometeorology    February 26, 2025   doi: 10.1007/s00484-025-02881-z
Giannetto C, Aragona F, Fazio F, Piccione G, Giudice E, Arfuso F, Zumbo A.This study aimed to examine the impact of climatic conditions on feed intake, body weight, and rectal temperature in horses residing in the hot Summer Mediterranean climate zone. Eight gelding Italian Saddle horses, with an average body weight of 427.78 ± 54.55 kg and aged between 10 and 13 years, participated in the research. All horses were individually housed in a paddock (800 m/horse), and fed ad libitum. Food intake, body weight, and rectal temperature were recorded every 10 days at four 30-day collection periods in the year: January, April, July, and October. The formula adapted fr...
Climate change impact on blood haemogram in the horse: a three-year preliminary study.
Frontiers in veterinary science    December 16, 2024   Volume 11 1482268 doi: 10.3389/fvets.2024.1482268
Deniz Ö, Aragona F, Murphy BA, Tümer KÇ, Bozacı S, Fazio F.The global climatic changes pose a substantial threat to the well-being and productivity of both humans and animals. Unassigned: This study examined the impact of climate changes during different seasons over a 3-year monitoring period (2021-2023) on various blood parameters including, white blood cells (WBC), neutrophils, basophils, eosinophils, lymphocytes, and monocytes, hematocrit (HCT), hemoglobin (HGB), red blood cells (RBC), platelets (PLT), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular volume (MCV), and mean corpuscular hemoglobin (MCH). The study focused on 25 Tho...
Environmental Conditions in Equine Indoor Arenas: A Descriptive Study.
Journal of equine veterinary science    August 16, 2024   105165 doi: 10.1016/j.jevs.2024.105165
McGill S, Coleman R, Hayes M.Indoor arenas do not always include mechanical ventilation or stirring fans and occupancy by horses and humans can be sporadic and inconsistent, which creates a challenging space for understanding and predicting variations in temperature, moisture, and airflow. To understand the interior environment within indoor arenas, monitoring was conducted at 15 facilities within 200 kilometres of Lexington, KY. Environmental monitoring of dry bulb temperature, relative humidity, dew point temperature, air speeds, and solar radiation took place over 7 days in the winter and summer to examine temporal var...
Fluctuations in equine cutaneous pH and transepidermal water loss with time of day and ambient conditions.
Journal of equine veterinary science    June 29, 2024   105140 doi: 10.1016/j.jevs.2024.105140
Discepolo DR, Gaare E, Handlos G, Perry EB.Cutaneous pH and transepidermal water loss (TEWL) are commonly utilized measures in dermatological research as they provide information concerning barrier function. The importance of dermal health has become more evident in recent years. Accordingly, the aim of this work was to identify natural fluctuations in the biophysical parameters of healthy equine skin. Cutaneous pH and TEWL was collected on nine mares at 6:00 AM, 12:00 PM, and 6:00 PM daily for five days on the nose, withers, girth area, mid-back, and base of tail. Ambient temperature and humidity were measured at each collection. Stat...
Use of thermography in the long-term evaluation of scrotal surface temperature and its impact on seminal quality in stallions.
Reproduction in domestic animals = Zuchthygiene    April 3, 2024   Volume 59, Issue 4 e14556 doi: 10.1111/rda.14556
Freitas ML, Viana JHM, Dode MAN, Braga TRC, de Oliveira RA.Scrotal surface thermography is a non-invasive method for assessing testicular thermoregulation in stallions; however, few studies have explored the application of this technique concerning the thermal physiology of equine reproductive systems. This study aimed to evaluate the consistency of testicular thermoregulation in stallions over a year using thermography to measure the scrotal surface temperature (SST). Moreover, we assessed the best region for measuring the surface body temperature compared with the SST. Ten light-breed stallions were used in the experiment. Thermographic images of th...
Correlation and agreement between infrared thermography and a thermometer for equine body temperature measurements.
Veterinary world    December 20, 2023   Volume 16, Issue 12 2464-2470 doi: 10.14202/vetworld.2023.2464-2470
Lampang KN, Isawirodom A, Rungsri P.Body temperature is a vital sign that determines physical status. Infrared thermography (IRT) is more frequently used for assessing horses' temperature because of its ease of use and less contact with the horses, making it a safer measurement method. However, the accuracy of IRT remains unclear; therefore, this study aimed to assess the potential use of IRT as an alternative method for measuring horse body temperature. Unassigned: Temperatures were measured in 14 horses. A digital thermometer was used to collect rectal temperature (RT), whereas a thermographic camera was used for IRT at three ...
Interplay Among the Daily Rhythm of the Thyroid Hormones, Uncoupling Protein 1 and the Clock Gene Per2 in Horses.
Journal of equine veterinary science    April 21, 2023   Volume 126 104496 doi: 10.1016/j.jevs.2023.104496
During husbandry, domestic animals are exposed to many factors that can influence their circadian physiology organization leading to an increase in animals' discomfort. Thermal homeostasis is at the basis of animal wellness, the aim of the present study was to investigate the daily fluctuation of serum concentrations of 3,5,3'-triiodothyronine (T3) and thyroxine (T4) in association with the daily fluctuation of uncoupling protein 1 (UCP1) and clock gene Per2 in healthy horses housed in individual box, to improve the knowledge on this matter. Seven clinically healthy female Italian Saddle horse...
Heat stress in horses: a literature review.
International journal of biometeorology    April 15, 2023   Volume 67, Issue 6 957-973 doi: 10.1007/s00484-023-02467-7
Kang H, Zsoldos RR, Sole-Guitart A, Narayan E, Cawdell-Smith AJ, Gaughan JB.Healthy adult horses can balance accumulation and dissipation of body heat to maintain their body temperature between 37.5 and 38.5 °C, when they are in their thermoneutral zone (5 to 25 °C). However, under some circumstances, such as following strenuous exercise under hot, or hot and humid conditions, the accumulation of body heat exceeds dissipation and horses can suffer from heat stress. Prolonged or severe heat stress can lead to anhidrosis, heat stroke, or brain damage in the horse. To ameliorate the negative effects of high heat load in the body, early detection of heat stress and im...
Risk Factors for Epistaxis in Thoroughbred Flat Races in Japan (2001-2020).
Animals : an open access journal from MDPI    April 14, 2023   Volume 13, Issue 8 doi: 10.3390/ani13081348
Sugiyama F, Takahashi Y, Nomura M, Ebisuda Y, Mukai K, Yoshida T.We investigated the risk factors for epistaxis in Japanese flat races over a 20-year period. The veterinary records of horses identified as having epistaxis by endoscopy on the race day, and the official racing records of all flat races from April to September between 2001 and 2020, were reviewed. The racecourses (n = 10), surface type, surface condition, race class, race distance, race year, sex, age, two training centers, ambient temperature, and body weight on race days were assessed using multivariable logistic regression (p < 0.05). Of 475,709 race starts, 616 (1.30 cases per 1000 starts;...
Invited review: The speed-duration relationship across the animal kingdom.
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology    February 4, 2023   Volume 279 111387 doi: 10.1016/j.cbpa.2023.111387
Burnley M.The parameters of the hyperbolic speed-duration relationship (the asymptote critical speed, CS, and the curvature constant, D') provide estimates of the maximal steady state speed (CS) and the distance an animal can run, swim, or fly at speeds above CS before it is forced to slow down or stop (D'). The speed-duration relationship has been directly studied in humans, horses, mice and rats. The technical difficulties with treadmill running in dogs and the relatively short greyhound race durations means that, perhaps surprisingly, it has not been assessed in dogs. The endurance capabilities of li...
Changes in Hair Coat Length and Diameter in Blanketed and Nonblanketed Adult Horses in the Winter.
Journal of equine veterinary science    December 9, 2022   Volume 120 104191 doi: 10.1016/j.jevs.2022.104191
DeBoer M, Konop A, Fisher B.Horses are often blanketed during cold weather for numerous reasons including assisting thermoregulation, cleanliness, and anecdotally to decrease hair coat length. However, the impact of blanketing on the hair coat has yet to be evaluated. The objective of this study was to evaluate changes in hair coat length and diameter over time in blanketed and nonblanketed horses during winter. In October 2019, 16 mature adult horses were blocked by breed and BCS and randomly assigned to a blanketed (n = 8) or nonblanketed (n = 8) treatment; blankets were placed. Data was collected between October 2...
A survey on the use of rugs in Australian horses.
Australian veterinary journal    November 27, 2022   Volume 101, Issue 1-2 9-26 doi: 10.1111/avj.13219
Cox EG, Bell R, Greer RM, Jeffcott LB.The practice of horse rugging has important implications for horse welfare and performance, but in Australia, rugging practices have not been properly documented. The objective of this study was to obtain an understanding of Australian horse rugging practices. An online survey reviewed the management of 2659 Australian horses over 12 months. Of the reported horses a total of 84.8% were rugged, although most owners (69.5%) indicated that they were unsure whether horses needed to be rugged at all. Rugging was felt by 59.8% of owners to be a requirement for horses in competition. Riding and com...
A Modified Schirmer Tear Test in Clinically Normal Horses: Measurement at 30 Seconds versus 60 Seconds.
Journal of equine veterinary science    November 4, 2022   Volume 120 104162 doi: 10.1016/j.jevs.2022.104162
Martín-Suárez EM, Mesa P, Portillo M, Morgaz J, Moreno O, Guisado A, Galán-Rodríguez A.This study sought to compare the Schirmer Tear Test (STT)-1 results at 30 (STT30) versus 60 (STT60) seconds in healthy horses. This study included a total of 56 healthy horses. STT-1 was performed in both eyes, right eye first, and the wetting lengths were measured in STT30 and STT60. To evaluate the reduction of the initial reflex phase, the wetting length velocity was measured during the first 30 seconds. The effects of eye, age, weight, sex, and ambient temperature and humidity on STT values were evaluated. Mean (standard deviation) STT30 and STT60 were 19.06 (3.88) and 24.26 (4.50) mm. The...
Effects of different winter climates in Japan on body composition of young Thoroughbreds in training.
The Journal of veterinary medical science    October 14, 2022   Volume 84, Issue 12 1585-1594 doi: 10.1292/jvms.22-0378
Ishimaru M, Matsui A, Seki K, Korosue K, Akiyama K, Mizukami H, Yoshida T, Taya K.Changes in the body composition of 50 Thoroughbreds colts and fillies, born between 2004 and 2010, were compared between those reared at the Hidaka Training and Research Center (Hidaka), Hokkaido, which is extremely cold in winter, and those reared at the Miyazaki Yearling Training Farm (Miyazaki), Kyushu, which is mildly cold in winter. The horses were divided into two sex groups and reared and trained in Hidaka or Miyazaki for 7 months from October of one year of age to April of two years of age. Body weight (BW), rump fat thickness (RFT), fat-free mass (FFM), and percentage of fat (%F) were...
Equine Rolling Behavior: Thermoregulation Mechanism After Exercise and Substrate Preference.
Journal of applied animal welfare science : JAAWS    October 10, 2022   1-10 doi: 10.1080/10888705.2022.2132825
Rolling is a natural behavior of equids that is beneficial to their well-being. However, more intensive domestic environments offer fewer opportunities for animals to roll, neglecting its importance. We believe that the inclusion of recreational areas for animals, containing substrates and conditions that encourage the occurrence of natural behaviors, is essential to promote their well-being. Therefore, we evaluated the preference of horses (; n = 8) and mules (E. asinus x E. caballus; n = 8) for different rolling substrates (sand, grass, manure), and the influence of this behavior on thermo...
High ambient temperature and humidity associated with early embryonic loss after embryo transfer in mares.
Theriogenology    May 24, 2022   Volume 188 37-42 doi: 10.1016/j.theriogenology.2022.05.014
Yu K, Pfeiffer C, Burden C, Krekeler N, Marth C.Increased ambient temperatures has been identified to contribute to reproductive outcomes for several domesticated species, but its impact on equine reproduction has not been previously investigated. Therefore, this study aimed to examine the relationship between ambient climatic conditions, as measured by temperature-humidity index (THI) between day 7 and day 14 of gestation, and early embryonic loss (EEL) in recipient mares undergoing embryo transfer. The study examined records from 834 embryo transfers at an equine breeding facility in Victoria, Australia. Early embryonic loss was defined a...
The effect of training on infrared thermographic images of the forelimb and hindlimb joints of healthy racehorses.
Polish journal of veterinary sciences    May 17, 2022   Volume 25, Issue 1 83-92 doi: 10.24425/pjvs.2022.140844
Soroko M, Górniak W, Godlewska M, Howell K.The aim of this study was to evaluate the influence of training on body surface temperature over the joints in racehorses, measured by infrared thermography. The study involved monitoring of 14 Thoroughbred racehorses in 6 imaging sessions over a period of 3 months. Temperature measurements of the forelimb and hindlimb joints were made before and just after training. Joint temperature of limbs increased significantly after training. Environmental temperature had a statistically significant influence on surface temperature over the joints. The lowest surface temperatures were recorded over the ...
Environmental influences on mare reproductive loss syndrome: Do they fit with a toxin as the causative agent?
Journal of equine veterinary science    April 28, 2022   Volume 114 104001 doi: 10.1016/j.jevs.2022.104001
Burns SJ, Westerman AG, Harrison LR.Mare Reproductive Loss Syndrome (MRLS) is the term given to abortions in mares associated with Eastern Tent caterpillars (ETC; Malacosoma americanum). This paper aims to examine if the hypothesis for a toxin as the cause of MRLS holds up to testing using Westerman's Correlates of Causal Strength of Evidence (WCCSE) and fits with known environmental factors that influence the occurrence of MRLS. Using WCCSE all correlates fit with a toxin as a potential causative agent. Environmental factors also fitted with this hypothesis. MRLS events were associated with higher than normal ambient temperatur...
Genome-Wide Detection of Copy Number Variants in Chinese Indigenous Horse Breeds and Verification of CNV-Overlapped Genes Related to Heat Adaptation of the Jinjiang Horse.
Genes    March 28, 2022   Volume 13, Issue 4 603 doi: 10.3390/genes13040603
Wang M, Liu Y, Bi X, Ma H, Zeng G, Guo J, Guo M, Ling Y, Zhao C.In the present study, genome-wide CNVs were detected in a total of 301 samples from 10 Chinese indigenous horse breeds using the Illumina Equine SNP70 Bead Array, and the candidate genes related to adaptability to high temperature and humidity in Jinjiang horses were identified and validated. We determined a total of 577 CNVs ranging in size from 1.06 Kb to 2023.07 Kb on the 31 pairs of autosomes. By aggregating the overlapping CNVs for each breed, a total of 495 CNVRs were detected in the 10 Chinese horse breeds. As many as 211 breed-specific CNVRs were determined, of which 64 were found in t...
Continuous Monitoring of the Thermoregulatory Response in Endurance Horses and Trotter Horses During Field Exercise: Baselining for Future Hot Weather Studies.
Frontiers in physiology    August 26, 2021   Volume 12 708737 doi: 10.3389/fphys.2021.708737
Verdegaal EJMM, Howarth GS, McWhorter TJ, Boshuizen B, Franklin SH, Vidal Moreno de Vega C, Jonas SE, Folwell LE, Delesalle CJG.Establishing proper policies regarding the recognition and prevention of equine heat stress becomes increasingly important, especially in the face of global warming. To assist this, a detailed view of the variability of equine thermoregulation during field exercise and recovery is essential. 13 endurance horses and 12 trotter horses were equipped with continuous monitoring devices [gastrointestinal (GI) pill, heartrate (HR) monitor, and global positioning system] and monitored under cool weather conditions during four endurance rides over a total of 80 km (40 km loops) and intense trotter trac...
Clinical insights: Preparing for the Tokyo Olympics-Ensuring health and welfare of equine athletes in the face of heat and humidity.
Equine veterinary journal    May 5, 2021   Volume 53, Issue 4 635-637 doi: 10.1111/evj.13446
Elliott C.No abstract available
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