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Topic:Disease Prevalence

Disease prevalence in horses refers to the frequency and distribution of various diseases within equine populations. Understanding disease prevalence is essential for identifying health trends, assessing risk factors, and implementing effective disease management and prevention strategies. Common diseases affecting horses include equine influenza, equine herpesvirus, strangles, and equine infectious anemia. The study of disease prevalence involves collecting and analyzing data from veterinary reports, field studies, and laboratory diagnostics. This page compiles peer-reviewed research studies and scholarly articles that investigate the patterns, causes, and implications of disease prevalence in horses, providing insights into equine health management and epidemiology.
Identification and phylogenetic analysis of Mannheimia haemolytica isolated from nasal samples of horses.
Folia microbiologica    September 3, 2026   doi: 10.1007/s12223-026-01578-z
Yeni DK, Paksoy Y, Balevi A, Büyük F, Ashraf A, Padron B, Toslak EE.The Mannheimia genus, which is part of the upper respiratory tract microbiota, harbours species that have the potential to cause progressive infections, such as haemorrhagic septicaemia and enzootic pneumonia, when the host immune system is suppressed. Among these species, Mannheimia haemolytica is considered the most pathogenic, and horses can be a host for this agent. This study aimed to investigate the occurrence of Mannheimia haemolytica in horses with and without respiratory clinical signs and to assess the potential pathogenic significance of isolates recovered from animals through serot...
Equine piroplasmosis in China, 2000 to 2025: a systematic review and meta-analysis of prevalence and risk factors.
Veterinary journal (London, England : 1997)    August 31, 2026   106866 doi: 10.1016/j.tvjl.2026.106866
Jiao D, Wei W, Dong X, Shi R, Sa R, Ma C, Bian P, Liu K, Wang L, Wei Z, Jiang H, Wang R.Equine piroplasmosis (EP) is a tick-borne disease of equids caused by Theileria equi, Babesia caballi, and Theileria haneyi. We conducted a PRISMA-guided systematic review and meta-analysis to estimate pooled prevalence in China and identify associated risk factors. PubMed, Web of Science, ScienceDirect, CNKI, Wanfang, and VIP were searched through 15 August 2025. Of 2,626 records identified, 80 studies met the inclusion criteria. Random-effects models were used to pool prevalence estimates, and prespecified subgroup analyses and univariate meta-regression were used to explore heterogeneity an...
Prevalence and Molecular Characterization of Enterocytozoon bieneusi in Horses From Northern and Eastern China: Investigation of Associated Risk Factors and Assessment of Zoonotic Potential.
Transboundary and emerging diseases    August 31, 2026   Volume 2026, Issue 1 e1144348 doi: 10.1155/tbed/1144348
Peng X, Zhang Z, Yan C, Zhu F, Qin W, Zhao W, Ke J.Enterocytozoon bieneusi is a zoonotic microsporidian threatening public health, yet epidemiological data on equine infections in China are scarce despite frequent human-horse contact. In this study, 412 fresh horse fecal samples were collected from four Chinese regions covering four rearing systems and were screened for E. bieneusi internal transcribed spacer (ITS) sequences via nested PCR, with risk factor and phylogenetic analyses conducted. The overall infection prevalence reached 15.0% (62/412), varying geographically from 9.7% (Wenzhou) to 29.3% (Qiqihar). Foals <1 year and male horses ex...
Comments on: ‘Prevalence and degree of orotracheal intubation-related tracheal lesions in horses’.
Equine veterinary journal    August 27, 2026   doi: 10.1002/evj.70320
Gittel C, Kaartinen J, Leech J, de Grauw JC.No abstract available
Allele frequencies of 7 inherited disorders in performance and random cohorts of American Quarter Horses (2020-2024).
Journal of the American Veterinary Medical Association    August 21, 2026   1-7 doi: 10.2460/javma.26.04.0256
Brown BN, Hughes S, Le TM, Tatar NP, Grahn JC, Carrillo-Alvarez M, Bellone RR, Finno CJ.To estimate allele frequencies for genetic diseases in the American Quarter Horse (QH), including hyperkalemic periodic paralysis (HYPP), type 1 polysaccharide storage myopathy (PSSM1), malignant hyperthermia (MH), myosin-heavy chain myopathy (MYHM), glycogen branching enzyme deficiency (GBED), hereditary equine regional dermal asthenia (HERDA), and equine juvenile spinocerebellar ataxia (EJSCA), and to compare these frequencies over time among elite performance subgroups and a random cohort of registered horses. Unassigned: In this prospective genetic survey, DNA was extracted from 300 elite ...
Epidemiological study of strongyle infections in equines in Egypt: prevalence and risk factors.
Frontiers in veterinary science    August 19, 2026   Volume 13 1901312 doi: 10.3389/fvets.2026.1901312
Selim A, Marzok M, Gattan HS, Alruhaili MH, Ismail H, Almubarak AI, Hereba AM.Equine strongylosis is one of the most prevalent gastrointestinal parasitic diseases affecting horses and donkeys worldwide, leading to reduced health, productivity, and working performance. This study aimed to determine the prevalence of gastrointestinal strongyle infection and identify associated risk factors among equines in three Egyptian governorates. Unassigned: A cross-sectional study was conducted on 540 horses and donkeys. Fresh fecal samples were collected and examined using the magnesium sulfate flotation technique for the detection of strongyle eggs. Associations between infection ...
Semi-extensive management system drives lower prevalence of equine orodental disorders.
Frontiers in veterinary science    August 18, 2026   Volume 13 1827412 doi: 10.3389/fvets.2026.1827412
Kunz JR, Peres Mendes R, Granella MCS, Hartl B, de Souza AF, Fonteque JH, Kau-Strebinger S.The prevalence of orodental disorders in horses kept under different management systems is lacking. More detailed knowledge is needed to support targeted therapeutic and prophylactic approaches, as these conditions play a crucial role in maintaining equine health and welfare. This study aimed to compare the prevalence of orodental disorders in horses raised, kept, and fed under extensive, semi-extensive, or intensive management systems. Unassigned: Sixty-nine clinically healthy adult crossbred horses with no history of dental treatment were examined, with 23 horses included in each study group...
Leishmania spp. infection in horses in an endemic area of canine leishmaniosis (north-western Algeria).
Research in veterinary science    August 17, 2026   Volume 211 106365 doi: 10.1016/j.rvsc.2026.106365
Bia T, Marteles-Aragüés D, Sánchez C, Bernardo L, Carrillo E, Solana JC, Manseur H, Zait H, Lafri I, Villanueva-Saz S, Moreno J.Leishmaniosis is a parasitic disease caused by obligate intracellular protozoa of the genus Leishmania. It affects humans, canids, and rodents in endemic areas and is transmitted through the bites of infected sand flies. This study aimed to estimate the prevalence of equine leishmaniosis in horses from Tiaret Province, northwestern Algeria, an area endemic for canine leishmaniosis, and to identify the main risk factors associated with infection. The study included 75 local Barb horses and employed molecular and serological techniques for the detection of Leishmania infection. Among the sampled...
Factors Associated With Corneal Ulceration in Horses: 420 Cases (2020-2023). Galindo VA, Fielding L.To evaluate how environmental and other risk factors are associated with the prevalence of corneal ulceration in horses presenting to equine emergency veterinarians in the Western United States. Methods: Retrospective study conducted from May 2020 to November 2023. Methods: Equine field and hospital practice in the Western United States. Methods: Four hundred twenty horses with corneal ulceration, confirmed by clinical examination and fluorescein staining, of any underlying cause. Methods: None. Results: The primary study outcomes were the association between environmental factors (wind speed,...
Molecular Detection of Anaplasma spp. in Horses From Golestan Province, Northern Iran.
Veterinary medicine and science    August 6, 2026   Volume 12, Issue 5 e71124 doi: 10.1002/vms3.71124
Rad AM, Rahimi H, Changizi E, Staji H.Anaplasmosis is a bacterial zoonotic disease that affects both humans and many animals. In horses, anaplasmosis manifests as a seasonal, infectious, and febrile disease caused by bacteria of the genus Anaplasma. Since the transmission of this disease is primarily mediated by arthropods such as ticks, it holds significant public health importance. Objective: The present study aims to investigate the prevalence of anaplasmosis in horses in northern Iran (Golestan Province). Methods: A total of 350 blood samples were randomly collected from asymptomatic horses between December 2023 and April 2024...
Risk Factors for Disease in Military Working Equines.
Military medicine    August 6, 2026   Volume 191, Issue Supplement_1 16-21 doi: 10.1093/milmed/usaf625
LaDouceur E, Raynor K, Chilcoat C, Crawford J, Heitz E.Diseases can impact Military Working Equids (MWEs) readiness and limit days in service. The objectives of this study are to categorize diseases in MWEs and evaluate potential risk factors for disease. Methods: Pathology reports from MWEs from 2005 to 2025 were retrieved from the Joint Pathology Center archives. Disease processes with ≥5 cases were evaluated for potential risk factors, including MWE breed, sex, neuter status, geographic location, and age at sampling. Results: Diagnoses included inflammation (n = 30), neoplasia/proliferation (n = 24), within normal limits (n = 12),...
Seroprevalence, risk factors and hematological parameters associated with Equine Infectious Anemia from Casanare, Colombia.
Journal of equine veterinary science    August 3, 2026   106108 doi: 10.1016/j.jevs.2026.106108
Puentes MMM, Guzman YL, Torreglosa JCT, Isaza PAL, Guzman-Barragán BL.Equine infectious anemia (EIA) is a persistent lentiviral disease with important sanitary and economic implications in tropical regions; however, epidemiological information in Colombia remains limited. Objective: To estimate seroprevalence, identify risk factors, and evaluate hematological alterations associated with EIA in horses from Casanare, Colombia. Methods: A cross-sectional study including 1,228 horses from 204 production units across 121 rural districts. Serological diagnosis was performed using agar gel immunodiffusion. Demographic and management data were obtained through an epidem...
First Molecular Survey of Equine Adenovirus Type 1 Infection Among Horses in Poland.
International journal of molecular sciences    August 1, 2026   Volume 27, Issue 15 6902 doi: 10.3390/ijms27156902
Stasiak K, Dunowska M, Rola J.Equine adenovirus type 1 (EAdV-1) infection has been recognised among horses worldwide. The virus predominantly targets respiratory tract but has also been recovered from other body systems. The objective of the study was to determine the frequency of EAdV-1 infection among selected horse populations in Poland and to estimate genetic variability within the viruses obtained. A quantitative PCR assay targeting a partial gene was used to screen 1150 nasal swabs collected from 1145 horses from 19 horse studs throughout Poland. The samples were collected opportunistically between 2012 and 2025. A ...
Correction to: Seroprevalence and risk factors of African horse sickness in equines in central Gondar, Ethiopia.
Veterinary research communications    July 23, 2026   Volume 50, Issue 5 476 doi: 10.1007/s11259-026-11406-x
Yehulashet AA, Wondmnew F, Abat AS, Alamerew EA, Birhan M, Tafere FA, Malede BA, Sechu A, Mersha AC, Tsehay EM, Temesgen AB.No abstract available
Tick-borne pathogens in a nature reserve area in the Netherlands: serological and molecular assessments in large herbivores and Ixodes ricinus ticks.
Ticks and tick-borne diseases    July 23, 2026   Volume 17, Issue 4 102686 doi: 10.1016/j.ttbdis.2026.102686
Marcelino I, Vriens R, Hakze-van der Honing RW, Lesiczka P, de Vries A, Graham H, Streng K, Cornelissen P, Uiterwijk M, Monti G, Sprong H....Ticks are vectors of a wide range of pathogens, relevant to both veterinary and public health. Although tick surveillance is frequently conducted in the Netherlands, data on tick-borne pathogens in free-living herbivores remains limited, leaving the impact of these pathogens on animal health poorly understood. This study aimed to characterise tick species and determine seroprevalence and infection prevalence of selected tick-borne pathogens in large herbivores at the Oostvaardersplassen nature reserve and questing ticks collected in the area. Between September 2023 and December 2024, blood was...
Penile and preputial pythiosis in Saudi Arabian horses: clinicopathological features and surgical outcomes.
Frontiers in veterinary science    July 22, 2026   Volume 13 1872270 doi: 10.3389/fvets.2026.1872270
El-Shafaey ES, Salem M, Awadin W, Mosbah E, Rizk MA, Meligy AMA, Shousha S, Aljazzar A, El Sebaei MG.Pythiosis, caused by , is a chronic granulomatous disease of horses characterized by invasive, ulcerative, and pyogranulomatous cutaneous lesions. Involvement of the external genitalia is uncommon and remains poorly characterized, particularly regarding environmental exposure and treatment outcomes. This study aimed to describe the epidemiological pattern, clinicopathological features, diagnostic findings, and surgical outcomes of penile and preputial genital pythiosis in a hospital-presented horse cohort. Unassigned: A retrospective study was conducted on 123 horses presented with cutaneous u...
Morphological investigation of myocardial sleeves with potential relevance to atrial fibrillation in Norwegian-Swedish Coldblooded Trotters.
Veterinary journal (London, England : 1997)    July 19, 2026   Volume 318 106784 doi: 10.1016/j.tvjl.2026.106784
Kallmyr-Mack A, Hellings IR, Press CM, Ibrahim L, van Loon G, Fintl C.Myocardial sleeves (MSs) are thin layers of cardiac muscle that extend from the atria into the caval and pulmonary veins. They are recognised as important structures in both initiating and maintaining atrial tachycardia, and especially atrial fibrillation (AF), which is the most clinically important cardiac arrhythmia in horses with a reported prevalence of 1.3-2% in Standardbred racehorses. In contrast, the Norwegian-Swedish Coldblooded Trotter (NSCT) has a much lower prevalence of AF despite experiencing similar physical demands. The purpose of this study was to describe the macro-, and micr...
Prevalence of equine lungworm (Dictyocaulus arnfieldi) infection and associated risk factors in Egypt.
Frontiers in veterinary science    July 17, 2026   Volume 13 1895496 doi: 10.3389/fvets.2026.1895496
Gattan HS, Marzok M, Alruhaili MH, Ismail H, Almubarak AI, Selim A. infection is a common parasitic respiratory disease of equines that can adversely affect animal health, welfare, and productivity. However, epidemiological information on this infection remains limited in many regions of Egypt. This study aimed to determine the prevalence of and identify associated risk factors among equines in three Egyptian governorates. Unassigned: A cross-sectional study was conducted on 415 equines, including 150 donkeys, 55 mules, and 210 horses. Animals were examined for , and data on species, age, body condition, and deworming history were collected. Associations bet...
Prevalence and Molecular Characterisation of Cryptosporidium spp. and Giardia duodenalis in Equines in Algeria.
Veterinary medicine and science    July 15, 2026   Volume 12, Issue 4 e71083 doi: 10.1002/vms3.71083
Reghaissia N, Al-Adilee YMS, Laatamna A, Dahmane A, Berghiche A, Samari H, Ansel S, Laadjailia Y, Gentekaki E, Kváč M, Holubová N, Tsaousis AD.Limited data are available on the epidemiology of Cryptosporidium spp. and Giardia duodenalis in equines in Algeria. No prior molecular data existed on giardiasis in this host population. Objective: This cross-sectional study aimed to determine the prevalence and molecular diversity of Cryptosporidium spp. and G. duodenalis in equines across multiple regions of Algeria, and to characterise species, subtypes and assemblages present. Methods: Between December 2021 and December 2022, 197 faecal samples were collected from 104 horses, 89 donkeys, 2 ponies, 1 zebra and 1 mule from 46 private farms,...
Primary photosensitization caused by Chamaecristas serpens var. serpens in horses, donkeys, cattle, and goats.
Toxicon : official journal of the International Society on Toxinology    July 11, 2026   Volume 281 109213 doi: 10.1016/j.toxicon.2026.109213
Silveira AM, Mendonça MFF, Oliveira Filho JC, Caymmi LG, Pimentel LA, Riet-Correa F.This paper reports an outbreak of Chamaecrista serpens var. serpens poisoning in Mangalarga Marchador horses, Pega donkeys, Nelore cattle, and Mambrina goats. The disease occurred in the municipality of Iaçu, in the semi-arid region of the state of Bahia, 15-30 days after the start of the rainy season, coinciding with the regrowth of the plant. The prevalence of the poisoning was 100% in donkeys and horses, 11.9% in cattle, and 38.8% in goats. Clinical signs were characterized by itching, edema, and crust formation, predominantly in areas of non-pigmented skin or with less hair coverage and i...
Seroprevalence and risk factors of African horse sickness in equines in central Gondar, Ethiopia.
Veterinary research communications    July 8, 2026   Volume 50, Issue 5 445 doi: 10.1007/s11259-026-11388-w
Yehulashet AA, Wondmnew F, Abat AS, Alamere EA, Birhan M, Tafere FA, Malede BA, Sechu A, Mersha AC, Tsehay EM, Temesgen AB.African horse sickness is a severe vector-borne viral disease of equines, associated with high mortality and substantial economic losses. In Ethiopia, where equines are essential for transport and agriculture, the disease remains endemic, yet epidemiological data are limited in many areas. This study aimed to estimate the seroprevalence of African horse sickness virus (AHSV) and describe variation in seropositivity across selected factors in equines in the Central Gondar Zone, Ethiopia. A cross-sectional study was conducted from December 2024 to August 2025, during which 384 serum samples were...
Tick-borne bacterial and protozoal pathogens in horses in Austria – A cross-sectional study on current and past infections and potential risk factors.
Ticks and tick-borne diseases    July 4, 2026   Volume 17, Issue 4 102680 doi: 10.1016/j.ttbdis.2026.102680
Pikalo J, Homann A, Goller A, Stimac I, Tichy A, Rother O, Cavalleri JM, Joachim A.Tick-borne bacterial and protozoal pathogens are a major threat to equine health worldwide. In Austria, studies on pathogens such as piroplasms, Anaplasma phagocytophilum and Borrelia spp. in horses are scarce and do not cover the countrywide horse population. To address this, blood samples from horses without prior record of infection with tick-borne pathogens (n = 588) were tested for the presence of Theileria equi, Babesia caballi or A. phagocytophilum DNA by PCR and for specific antibodies against these pathogens and Borrelia burgdorferi with commercially available assays. While no DNA of ...
Seroprevalence, Risk Factors, and Environmental Correlates of Babesia caballi, Toxoplasma gondii, and Coxiella burnetii in Equids from Southwestern Greece.
Pathogens (Basel, Switzerland)    July 3, 2026   Volume 15, Issue 7 703 doi: 10.3390/pathogens15070703
Touloudi A, Giannakopoulos A, Tyrnenopoulou P, Siasios A, Athanasakopoulou Z, Tsinopoulou G, Sofia M, Spyrou V, Fthenakis GC, Billinis C....Equids, primarily horses, are mostly used for recreational purposes, although in some rural areas they also serve as working animals, maintaining close and frequent contact with humans. Their risk of exposure to vector-borne and zoonotic pathogens can be affected by host-related factors, management practices and environmental conditions. This study aimed to investigate the seroprevalence and associated risk factors for infections by , , , and sensu lato in equids from Southwestern Greece. A total of 159 equids were tested using commercial serological assays. Weighted prevalence estimates were...
Prevalence of insulin dysregulation in a sport horse population as determined by traditional and stall-side testing.
Equine veterinary journal    July 1, 2026   doi: 10.1002/evj.70204
Hallowell KL, Horne CR, Miller CS, Tufts SR, Dembek K, Love KR, van Eps AW, Schnabel LV.The prevalence of insulin dysregulation (ID) has not been investigated in sport horses. Stall-side insulin assays offer convenience but need further validation. Objective: To determine the prevalence of and risk factors for ID in a sport horse population using an oral sugar test (OST) and assess agreement between stall-side and validated laboratory assays for insulin quantification. Methods: Prospective cross-sectional study. Methods: One hundred eighty sport horses presenting for orthopaedic evaluation at a university hospital (N = 140) or local farms (N = 40) were tested for ID using...
Prevalence of Clostridioides difficile in hospitalized neonatal mare-foal pairs from Alberta and direct comparison of fecal culture with toxin gene detection. Whitehead AE, Borges AS, Zakia LS, Yu S, Surette MG, Arroyo LG. is an important pathogen in the feces of adult horses and foals. It can be clinically diagnosed using several testing methods, including culture isolation and, more commonly, direct toxin gene detection from feces using molecular methods. The importance of monitoring shedding in foals or dams during hospitalization remains unclear. The objectives of this study were to determine the prevalence of fecal shedding in mare-foal pairs and to compare detection using isolation and a commercial real-time polymerase chain reaction (qPCR) for toxin A and B genes in feces. Foals and their mares admitte...
Parascaris spp. induced surgical colic in juvenile horses-A Scandinavian perspective.
Equine veterinary journal    June 16, 2026   doi: 10.1002/evj.70208
Tydén E, Risnes Hellings I, Toft Nielsen K, Bergren A, Holmgren I, Attin S, Pihl T, Kendall A, Hedberg Alm Y.Parascaris spp. commonly infects foals, and high burdens can cause fatal small intestinal impactions. Progressive anthelmintic resistance poses a risk for an increase of Parascaris spp. related disease in juvenile horses. Objective: To investigate Parascaris-related surgical intestinal lesions, with special emphasis on ascarid impactions, in Scandinavia by exploring (1) disease occurrence and (2) short-term survival rates after laparotomy in affected foals and yearlings. Methods: Restrospective case series. Methods: A retrospective clinical record search was conducted at six Scandinavian equin...
The Occurrence of Bacterial Infections in Equine Wounds and Abscesses in Horses from 2019 to 2023.
Veterinary sciences    June 16, 2026   Volume 13, Issue 6 584 doi: 10.3390/vetsci13060584
Kłopotowska J, Kalbhenn EM, Klein B, Stöckle SD, Merle R, Müller E, Gehlen H.Bacterial infections in equine wounds are common and can complicate treatment and healing. This five-year retrospective study examined 2844 samples from wounds and abscesses from horses in Germany (2019-2023), analyzing bacterial prevalence by care setting and geographical region. A total of 4464 bacterial isolates were identified, with β-hemolytic streptococci (20.6%), (15.6%), and (13.0%) being the most common. Methicillin-resistant (MRSA) accounted for 35.9% of isolates. Resistance to third- and fourth-generation cephalosporins was detected in 15.2% of isolates. Samples from clinics s...
Glandular gastric disease prevalence in equids (horses, donkeys, and mules) and other gastric findings: post-mortem comparative study.
Veterinary research forum : an international quarterly journal    June 15, 2026   Volume 17, Issue 6 373-380 doi: 10.30466/vrf.2025.2056590.4709
Medina Bolivar AL, Faleiros RR, Martinez Aranzales JR.Equine glandular gastric disease (EGGD) has limited prevalence studies and scarce understanding of the predisposing factors and pathophysiological mechanisms involved. The objectives of this study were to determine and compare the post-mortem prevalence of EGGD and other gastric lesions in Colombian equids, specifically horses, donkeys, and mules. The study utilized a sample of 300 equids stomachs, with 100 specimens from each group, collected immediately after slaughter. The post-mortem examination included a thorough inspection of the ventral fundus, adjacent area of the margo plicatus, and ...
Prevalence, molecular characterization and zoonotic potential of Cryptosporidium spp. in horses from diverse husbandry systems across four provinces of northern and eastern China.
Veterinary journal (London, England : 1997)    June 13, 2026   Volume 318 106745 doi: 10.1016/j.tvjl.2026.106745
Qin W, Zhu F, Guan Y, Zeng H, Zhang Z, Cui Y, Zhang K, Zhao W, Ke J.Cryptosporidium is an obligate intracellular zoonotic protozoan causing diarrheal disease in humans and animals globally. Horses have more frequent close contact with humans through equestrian sports, breeding, and cross-regional trade. However, comprehensive molecular epidemiological data on diverse equine husbandry systems in China are limited. We tested 412 fecal samples from horses across four Chinese provinces via small subunit ribosomal RNA (SSU rRNA) nested polymerase chain reaction (PCR). C. parvum and C. equi were subtyped using the 60 kDa glycoprotein (gp60) gene, and C. andersoni ...
Prevalence and spatial distribution of Brucella spp. seropositive equids from the state of Mato Grosso, Brazil.
Journal of equine veterinary science    June 13, 2026   106064 doi: 10.1016/j.jevs.2026.106064
Borges NF, Barros ML, Silva IS, Barddal JEI, de Freitas AFB, Sumiyoshi MH, Muraro LS, Gomes SSP, de Oliveira ACS, Ribeiro MG, de Aguiar DM.Brucellosis is a chronic infectious disease in equids, characterized by suppurative lesions in joints and bursae, with potential impacts on animal performance and public health. Objective: To investigate the prevalence and spatial distribution of Brucella spp. seropositive equids in the state of Mato Grosso, Brazil. Methods: A total of 3,858 serum samples from 3,230 horses, 602 mules, and 26 donkeys were analyzed using the Buffered Acidified Antigen Test (AAT) and Complement Fixation Test (CFT), following Brazilian Ministry of Agriculture guidelines. Samples were obtained from all municipaliti...
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