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Topic:Equine Science

Equine Science encompasses the study of horses and their management, health, and performance. This field integrates various scientific disciplines such as biology, genetics, nutrition, physiology, and veterinary medicine to understand and improve the well-being and capabilities of horses. Areas of focus include equine anatomy, reproduction, behavior, and disease prevention. Research in equine science aims to enhance horse care, optimize training and performance, and address health challenges. This page compiles peer-reviewed research studies and scholarly articles that explore the diverse aspects of equine science, providing insights into the latest advancements and methodologies in the field.
Causative mechanisms and functional correlates of MTT reduction in stallion spermatozoa.
Reproduction (Cambridge, England)    April 22, 2022   Volume 163, Issue 6 341-350 doi: 10.1530/REP-21-0464
Medica AJ, Gibb Z, Sheridan A, Harrison N, Aitken RJ.MTT is a commonly used cell vitality probe, due to its ability to form insoluble formazan deposits at cellular locations of intense oxidoreductase activity. Although this response is considered a reflection of mitochondrial redox activity, extra-mitochondrial sites of MTT reduction have been recognized within the spermatozoa of several mammalian species. Therefore, the aim of this study was to determine the major sites and causative mechanisms of MTT reduction in stallion spermatozoa. Our results show that stallion spermatozoa displayed substantial mitochondrial formazan deposition, as well as...
A genetic investigation of equine recurrent uveitis in the Icelandic horse breed.
Animal genetics    April 22, 2022   Volume 53, Issue 3 436-440 doi: 10.1111/age.13200
Hack Y, Henriksen ML, Pihl TH, Nielsen RK, Dwyer AE, Bellone RR.Equine recurrent uveitis (ERU) is an autoimmune disease defined by inflammation of the uveal tract of the eye. The cause of ERU is thought to be complex, involving both genetic and environmental factors. The purpose of this study was to investigate potential genetic risk factors for ERU in the Icelandic horse. Fifty-six Icelandic horses (11 affected with ERU and 45 controls) living in Denmark and the USA, eight years or older, were included in the study. A case-control GWAS was performed using the GGP Equine 80K array on the Illumina Infinium HD Beadchip using 40 horses. A mixed linear model a...
Social Box: Influence of a New Housing System on the Social Interactions of Stallions When Driven in Pairs.
Animals : an open access journal from MDPI    April 21, 2022   Volume 12, Issue 9 1077 doi: 10.3390/ani12091077
Gmel AI, Zollinger A, Wyss C, Bachmann I, Briefer Freymond S.In order to improve the housing conditions of stallions in individual boxes, we tested a so-called “social box” allowing increased physical contact between neighbouring horses. This study investigated whether housing stallions in social boxes changes the number of social interactions during carriage driving. We hypothesised that the stay in social boxes would decrease the number of unwanted social interactions between stallions when driven in pairs. Eight Franches-Montagnes breeding stallions were observed when driven in pairs with a “neutral” stallion housed in a so-called “conventi...
Genome-wide survey on three local horse populations with a focus on runs of homozygosity pattern.
Journal of animal breeding and genetics = Zeitschrift fur Tierzuchtung und Zuchtungsbiologie    April 21, 2022   Volume 139, Issue 5 540-555 doi: 10.1111/jbg.12680
Criscione A, Mastrangelo S, D'Alessandro E, Tumino S, Di Gerlando R, Zumbo A, Marletta D, Bordonaro S.Purosangue Orientale Siciliano, Sanfratellano and Siciliano represent the Sicilian equine genetic resource. This study aimed to investigate the genetic diversity, population structure and the pattern of autozygosity of Sicilian horse populations using genome-wide single-nucleotide polymorphism (SNP) data generated with the Illumina Equine SNP70 array. The genotyping data of 17 European and Middle East populations were also included in the study. The patterns of genetic differentiation, model-based clustering and Neighbour-Net showed the expected positioning of Sicilian populations within the w...
A quantitative method for the simultaneous detection of SR9009 and SR9011 in equine plasma using liquid chromatography-electrospray ionization-tandem mass spectrometry.
Drug testing and analysis    April 19, 2022   Volume 14, Issue 8 1532-1538 doi: 10.1002/dta.3271
Kwak YB, Yu J, Yoo HH.SR9009 and SR9011 are metabolic modulators pharmacologically targeting REV-ERB receptors as synthetic agonists. A liquid chromatography-tandem mass spectrometry method for the detection of SR9009 and SR9011 in equine plasma was developed and validated. Plasma samples were pretreated by protein precipitation with methanol and were loaded onto an ACQUITY ultra performance liquid chromatography high-strength silica C18 column (2.1 × 150 mm, 1.8 μm) for chromatographic separation. The mobile phase consisted of 5-mM ammonium formate (pH 3.0) in distilled water and 0.1% formic acid in ac...
Effect of short-term artificial light and transvaginal progesterone device on first ovulation in late transitional mares.
Journal of equine science    April 19, 2022   Volume 33, Issue 1 1-6 doi: 10.1294/jes.33.1
Bianchi CP, Bruno S, Videla Dorna I, Rodríguez E, Aba MA.In study I, plasma progesterone concentrations were evaluated in anoestrous mares that received an intravaginal progesterone release device (IPRD) for 10 days. Mares were divided into 3 groups based on the dosage of progesterone (0 g, n=3; 1.38 g, n=5; and 1.9 g, n=5). No statistical differences were found in plasma progesterone concentrations between the two doses tested. In study II, the effects of a protocol based on a short program of artificial light combined with an IPRD containing 1.38 g of progesterone on oestrous behaviour and onset of ovulation were evaluated. IPRDs were inserted int...
Effect of single and double hemitenotomy on equine deep digital flexor tendon length and strength in experimental load challenges.
Veterinary surgery : VS    April 18, 2022   Volume 51, Issue 7 1153-1160 doi: 10.1111/vsu.13808
Zetterström SM, Boone LH, Farag R, Weimar WH, Caldwell FJ.To evaluate a double hemitenotomy (DHT) technique as an alternative to complete deep digital flexor (DDFT) tenotomy. Methods: Experimental ex vivo study. Methods: Isolated DDFTs (n = 30) and cadaveric forelimbs (n = 16). Methods: In part 1, 15 isolated DDFT pairs were used. Two hemitenotomies were created in 1 DDFT while the other served as reference. Monotonic tensile load was applied. Tendon lengthening, load reduction, and load at failure were recorded. In part 2, 16 cadaveric forelimb pairs were subjected to DHT followed by complete tenotomy (CT) under monotonic compressive load. Diffe...
TRPV4 activation enhances compressive properties and glycosaminoglycan deposition of equine neocartilage sheets.
Osteoarthritis and cartilage open    April 18, 2022   Volume 4, Issue 2 100263 doi: 10.1016/j.ocarto.2022.100263
López-Jiménez C, Chiu LLY, Waldman SD, Guilak F, Koch TG.To evaluate the effect of Transient Receptor Potential Vanilloid 4 (TRPV4) cation channel modulation on mesenchymal stromal cell (MSC)-derived neocartilage. Unassigned: RT-PCR was performed to evaluate mRNA levels of chondrogenic, hypertrophic and candidate mechanoresponsive genes in equine neocartilage sheets exposed to pulses of the TRPV4 agonist (GSK101) at different concentrations (N ​= ​10). Biochemical assays and mechanical tests (double indentation and unconfined compression) evaluated neocartilage properties (N ​= ​5). Unassigned: GSK101 treatment (1 ​nM) increased leve...
Laterality in Horse Training: Psychological and Physical Balance and Coordination and Strength Rather Than Straightness.
Animals : an open access journal from MDPI    April 16, 2022   Volume 12, Issue 8 1042 doi: 10.3390/ani12081042
Krueger K, Schwarz S, Marr I, Farmer K.For centuries, a goal of training in many equestrian disciplines has been to straighten the horse, which is considered a key element in achieving its responsiveness and suppleness. However, laterality is a naturally occurring phenomenon in horses and encompasses body asymmetry, motor laterality and sensory laterality. Furthermore, forcibly counterbalancing motor laterality has been considered a cause of psychological imbalance in humans. Perhaps asymmetry and laterality should rather be accepted, with a focus on training psychological and physical balance, coordination and equal strength on bo...
‘Pivotal’ equine ID consultation launched.
The Veterinary record    April 15, 2022   Volume 190, Issue 8 308 doi: 10.1002/vetr.1695
Mills G.No abstract available
Correction: Maskato et al. Prospective Feasibility and Revalidation of the Equine Acute Abdominal Pain Scale (EAAPS) in Clinical Cases of Colic in Horses. Animals 2020, 10, 2242.
Animals : an open access journal from MDPI    April 13, 2022   Volume 12, Issue 8 1001 doi: 10.3390/ani12081001
Maskato Y, Dugdale AHA, Singer ER, Kelmer G, Sutton GA.The authors wish to make the following corrections to this paper [...].
Establishment and characterization of equine mammary organoids using a method translatable to other non-traditional model species.
Development (Cambridge, England)    April 12, 2022   Volume 149, Issue 7 dev200412 doi: 10.1242/dev.200412
Bartlett AP, Harman RM, Weiss JR, Van de Walle GR.Mammary organoid (MaO) models are only available for a few traditional model organisms, limiting our ability to investigate mammary gland development and cancer across mammals. This study established equine mammary organoids (EqMaOs) from cryopreserved mammary tissue, in which mammary tissue fragments were isolated and embedded into a 3D matrix to produce EqMaOs. We evaluated viability, proliferation and budding capacity of EqMaOs at different time points during culture, showing that although the number of proliferative cells decreased over time, viability was maintained and budding increased....
Does Carrying a Rider Change Motor and Sensory Laterality in Horses?
Animals : an open access journal from MDPI    April 12, 2022   Volume 12, Issue 8 992 doi: 10.3390/ani12080992
Schwarz S, Marr I, Farmer K, Graf K, Stefanski V, Krueger K.Laterality in horses has been studied in recent decades. Although most horses are kept for riding purposes, there has been almost no research on how laterality may be affected by carrying a rider. In this study, 23 horses were tested for lateral preferences, both with and without a rider, in three different experiments. The rider gave minimal aids and rode on a long rein to allow the horse free choice. Firstly, motor laterality was assessed by observing forelimb preference when stepping over a pole. Secondly, sensory laterality was assessed by observing perceptual side preferences when the hor...
Calm with horses? A systematic review of animal-assisted interventions for improving social functioning in children with autism.
Autism : the international journal of research and practice    April 11, 2022   Volume 26, Issue 6 1320-1340 doi: 10.1177/13623613221085338
Sissons JH, Blakemore E, Shafi H, Skotny N, Lloyd DM.Children with autism typically experience difficulties interacting socially with others when compared to their non-autistic peers. Establishing how effective interventions are for improving social functioning is important to help inform what should be offered to children with autism. This study reviewed how effective interventions that involved interaction with a live animal, known as animal-assisted interventions, are in improving social functioning in children with autism. A systematic search of the evidence on this topic found nine studies, which were explored for the effectiveness of anima...
Molecular Dynamics and Evolution of Centromeres in the Genus Equus.
International journal of molecular sciences    April 10, 2022   Volume 23, Issue 8 4183 doi: 10.3390/ijms23084183
The centromere is the chromosomal locus essential for proper chromosome segregation. While the centromeric function is well conserved and epigenetically specified, centromeric DNA sequences are typically composed of satellite DNA and represent the most rapidly evolving sequences in eukaryotic genomes. The presence of satellite sequences at centromeres hampered the comprehensive molecular analysis of these enigmatic loci. The discovery of functional centromeres completely devoid of satellite repetitions and fixed in some animal and plant species represented a turning point in centromere biology...
A CONSORT-guided, randomized, double-blind, controlled pilot clinical trial of inhaled lidocaine for the treatment of equine asthma. Mahalingam-Dhingra A, Mazan MR, Bedenice D, Ceresia M, Minuto J, Deveney EF.There are limited options for treatment of the common disease, equine asthma. The aim of this study was to estimate the feasibility and potential efficacy of using nebulized lidocaine for treating equine asthma, while at the same time treating a separate cohort of asthmatic horses with inhaled budesonide. Nineteen horses with a history consistent with equine asthma were recruited from our referral population for a double-blind, randomized, controlled pilot clinical trial using Consolidated Standards of Reporting Trials (CONSORT) guidelines. After screening, 16 horses met the inclusion criteria...
Evaluation of a 3D Computer Model of the Equine Paranasal Sinuses as a Tool for Veterinary Anatomy Education.
Journal of veterinary medical education    April 6, 2022   Volume 50, Issue 2 234-242 doi: 10.3138/jvme-2021-0134
Canright A, Bescoby S, Dickson J.Detailed knowledge of anatomical systems is vital for clinical veterinary practice. However, students often find it difficult to transfer skills learned from textbooks to real-life practice. In this study, a three-dimensional computer model representing equine paranasal sinus anatomy (3D-ESM) was created and evaluated for its contribution to student understanding of the 3D dynamic nature of the system. Veterinary students and equine professionals at the University of Bristol were randomly allocated into experimental (3D model) and control (2D lecture) groups. A pre-/post-study design was used ...
AK-2011 strain for the development of a vaccine against equine rhinopneumonitis.
Transboundary and emerging diseases    April 5, 2022   Volume 69, Issue 5 e1972-e1981 doi: 10.1111/tbed.14531
Abisheva A, Abishov A, Khairullaeva K, Shynybayev K, Kalissynov B, Maikhin K, Kydyrmanov A, Karamendin K, Valdovska A, Syrym N.Equine rhinopneumonitis is an acute, highly contagious disease found virtually worldwide. The purpose of the studies presented in this paper is to develop a technology for the manufacture of a cell-derived equine rhinopneumonitis vaccine, as well as to assess the safety and immunogenicity of the newly developed vaccine in laboratory animals model. The object of the studies was the AK-2011 strain isolated from the horses suffering from rhinopneumonitis during an outbreak of abortions. The viability of the AK-2011 strain was assessed using a continuous line of calf trachea cells, a continuous li...
Comparison of Donkey, Pony, and Horse Dorsal Profiles and Head Shapes Using Geometric Morphometrics.
Animals : an open access journal from MDPI    April 5, 2022   Volume 12, Issue 7 931 doi: 10.3390/ani12070931
Maśko M, Wierzbicka M, Zdrojkowski Ł, Jasiński T, Sikorska U, Pawliński B, Domino M.Since donkey breeding has increased due to their variety of uses, welfare evaluation has become more important. This study aimed to compare donkey, pony, and horse dorsal profiles and head shapes using geometric morphometrics (GM). Photographs of 14 donkeys, 14 ponies, and 14 horses were analyzed using GM, including the sliding semilandmarks method. The variations in the first three principal components (PCs) were PC1: 57.16%, PC2: 16.05%, and PC3: 8.31% for the dorsal profiles and PC1: 44.77%, PC2: 13.46%, and PC3: 7.66% for the head shapes. Both the dorsal profiles and head shapes differed b...
Preantral follicle population and distribution in the horse ovary.
Reproduction & fertility    April 4, 2022   Volume 3, Issue 2 90-102 doi: 10.1530/RAF-21-0100
Hyde KA, Aguiar FLN, Alves BG, Alves KA, Gastal GDA, Gastal MO, Gastal EL.Characterization of the ovarian preantral follicle population is a necessary step to improve understanding of folliculogenesis and ovarian physiology. Therefore, in the present study, the preantral follicle population in the equine ovary in young and old mares was investigated according to follicular morphology, follicular class, distance from the geometric center using ovarian maps, and follicular density within ovarian portions (lateral vs intermediary) and regions (dorsal vs ventral). Ovaries were collected from an abattoir and histologically processed for evaluation, and the follicle popul...
Gait quality scoring data of Franches-Montagnes stallions at walk and trot on a treadmill by experts of the breed and their reliability.
Data in brief    April 1, 2022   Volume 42 108123 doi: 10.1016/j.dib.2022.108123
Gmel AI, Gmel G, Weishaupt MA, Neuditschko M.This article presents the data obtained from the scoring of 24 stallions of the Franches-Montagnes (FM) horse breed by six experts of this breed. The experts scored six traits at walk and eight at trot from the video recordings of these stallions walking and trotting on a treadmill during an incremental speed test. The scores were given on a scale of one to nine. All experts scored the same videos twice (two scoring tests) with a time interval of two years, and without feedback from the first scoring. Video sequences were presented in a different order between first and second scoring. The int...
Expression of the Heterotrimeric GP2/GP3/GP4 Spike of an Arterivirus in Mammalian Cells.
Viruses    April 1, 2022   Volume 14, Issue 4 doi: 10.3390/v14040749
Matczuk AK, Zhang M, Veit M, Ugorski M.Equine arteritis virus (EAV), an enveloped positive-strand RNA virus, is an important pathogen of horses and the prototype member of the Arteiviridae family. Unlike many other enveloped viruses, which possess homotrimeric spikes, the spike responsible for cellular tropism in Arteriviruses is a heterotrimer composed of 3 glycoproteins: GP2, GP3, and GP4. Together with the hydrophobic protein E they are the minor components of virus particles. We describe the expression of all 3 minor glycoproteins, each equipped with a different tag, from a multi-cassette system in mammalian BHK-21 cells. Copre...
Reducing the incidence of domestic violence: An observational study of an equine-assisted intervention.
Family process    March 30, 2022   Volume 61, Issue 2 549-570 doi: 10.1111/famp.12768
Hemingway A, Sullivan K.This paper is presenting results from an observational study which has measured the impact of an equine-assisted education (EAE) intervention on the future occurrence of domestic violence within the family over 1 year following completion of the intervention as part of the troubled families program. The data analyzed were collected by the local authority troubled family's team from the different agencies involved including crime, health, and social care data. The data were analyzed and compared across four groups, those families on the troubled families program who had a key worker with a mem...
Author Correction: The effect of stress and exercise on the learning performance of horses.
Scientific reports    March 29, 2022   Volume 12, Issue 1 5322 doi: 10.1038/s41598-022-09569-z
Henshall C, Randle H, Francis N, Freire R.No abstract available
Use of Equine-Assisted Services to Improve Outcomes Among At-Risk and Indigenous Youth: A Scoping Review.
Frontiers in public health    March 28, 2022   Volume 10 730644 doi: 10.3389/fpubh.2022.730644
Haig L, Skinner K.Equine-assisted services (EAS) are gaining popularity as ways to promote psychological health and social well-being. EAS may show particular promise as culturally appropriate initiatives for at-risk Indigenous youth, as they are thought to align well with Indigenous ways of knowing which place emphasis on relationships between the land and all living beings. We seek to better understand previous uses of EAS as initiatives for at-risk youth populations, including Indigenous populations, and learn about which outcomes have been addressed in the literature with an EAS initiative by conducting a s...
Equine metabolism of the growth hormone secretagogue MK-0677 in vitro and in urine and plasma following oral administration.
Drug testing and analysis    March 25, 2022   Volume 14, Issue 7 1273-1290 doi: 10.1002/dta.3252
Cutler C, Viljanto M, Taylor P, Habershon-Butcher J, Van Eenoo P.Ibutamoren mesylate, or MK-0677, is an orally active, nonpeptide growth hormone secretagogue that has been developed to stimulate excretion of endogenous growth hormone. It has been evaluated for the treatment of a range of clinical conditions but is not available therapeutically. Nonetheless, MK-0677 is widely available to purchase online, sold as 'supplement' products. The mode of action and relative ease of purchase make MK-0677 a potential threat with regard to sports doping. The aim of this study was to investigate the metabolism of MK0677 in the horse following in vitro incubation and or...
Fluctuations of Physiological Variables during Conditioning of Lipizzan Fillies before Starting under Saddle.
Animals : an open access journal from MDPI    March 25, 2022   Volume 12, Issue 7 doi: 10.3390/ani12070836
Čebulj-Kadunc N, Frangež R, Kruljc P.Scientific studies on the physiological responses of young horses to workloads are limited. Therefore, the aim of our study was to determine the basal values of some cardiovascular, thermoregulatory, hematological, and biochemical parameters in 10 purebred Lipizzan fillies aged 4 years in the initial phase of training, and their responses to a graded workload, i.e., by lunging for 15 min in four exercise tests at 2-week intervals. The basal values of the measured parameters were within a range for warm-blooded horses and mostly increased after exercise in all four exercise tests. Resting heart...
Use of specific mitochondrial complex inhibitors to investigate mitochondrial involvement on horse sperm motility and ROS production.
Research in veterinary science    March 24, 2022   Volume 147 12-19 doi: 10.1016/j.rvsc.2022.03.017
Giaretta E, Mislei B, Martínez-Pastor F, Nesci S, Spinaci M, Galeati G, Nerozzi C, Mari G, Tamanini C, Bucci D.Equine spermatozoa highly rely on oxidative phosphorylation for their energy management. The present work aimed to characterize the role of mitochondria on horse sperm motility and ROS production by incubating spermatozoa with specific inhibitors of the different mitochondrial complexes. Equine spermatozoa were incubated 1 h and 3 h at 37 °C with: complex I inhibitor rotenone (5 μM, ROT), complex II inhibitor dimethyl-malonate (10 mM, DMM), complex III inhibitor antimycin A (1.8 μM, ANTI), the uncoupling agent carbonyl cyanide m-chlorophenyl hydrazine (5 μM, CCCP), ATP synthase inhi...
Metagenomic investigation of the equine faecal microbiome reveals extensive taxonomic diversity.
PeerJ    March 23, 2022   Volume 10 e13084 doi: 10.7717/peerj.13084
Gilroy R, Leng J, Ravi A, Adriaenssens EM, Oren A, Baker D, La Ragione RM, Proudman C, Pallen MJ.The horse plays crucial roles across the globe, including in horseracing, as a working and companion animal and as a food animal. The horse hindgut microbiome makes a key contribution in turning a high fibre diet into body mass and horsepower. However, despite its importance, the horse hindgut microbiome remains largely undefined. Here, we applied culture-independent shotgun metagenomics to thoroughbred equine faecal samples to deliver novel insights into this complex microbial community. We performed metagenomic sequencing on five equine faecal samples to construct 123 high- or medium-quality...
Outbreak of equine coronavirus disease in adult horses, Switzerland 2021.
Transboundary and emerging diseases    March 22, 2022   Volume 69, Issue 4 1691-1694 doi: 10.1111/tbed.14501
Hierweger MM, Remy-Wohlfender F, Franzen J, Koch MC, Blau D, Schoster A, Nicholson P, Gerber V, Gurtner C, Fouché N, Unger L, Seuberlich T.Coronaviruses are causing severe respiratory and enteric diseases in humans and animals. Here, we report an outbreak of equine coronavirus disease in adult horses, detected by a voluntary syndromic surveillance scheme for equine diseases in Switzerland. This scheme allowed a rapid concerted action to diagnose and contain the disease.
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