Analyze Diet

Topic:Statistical Analysis

Statistical analysis in equine research involves the application of mathematical techniques to collect, review, and interpret data related to horse health, behavior, and performance. This field encompasses a variety of methods, including descriptive statistics, inferential statistics, and multivariate analysis, to identify patterns, test hypotheses, and draw conclusions from equine data. Statistical analysis aids in understanding various aspects of equine science, such as disease prevalence, treatment efficacy, genetic traits, and performance metrics. This page aggregates peer-reviewed research studies and scholarly articles that explore the methodologies, applications, and outcomes of statistical analysis in the context of equine studies.
Evaluation of a wireless activity monitoring system to quantify locomotor activity in horses in experimental settings.
Equine veterinary journal    March 22, 2016   Volume 49, Issue 2 225-231 doi: 10.1111/evj.12568
Fries M, Montavon S, Spadavecchia C, Levionnois OL.Methods of evaluating locomotor activity can be useful in efforts to quantify behavioural activity in horses objectively. Objective: To evaluate whether an accelerometric device would be adequate to quantify locomotor activity and step frequency in horses, and to distinguish between different levels of activity and different gaits. Methods: Observational study in an experimental setting. Methods: Dual-mode (activity and step count) piezo-electric accelerometric devices were placed at each of 4 locations (head, withers, forelimb and hindlimb) in each of 6 horses performing different controlled ...
Hypoglycin A Concentrations in Maple Tree Species in the Netherlands and the Occurrence of Atypical Myopathy in Horses.
Journal of veterinary internal medicine    March 20, 2016   Volume 30, Issue 3 880-884 doi: 10.1111/jvim.13927
Westermann CM, van Leeuwen R, van Raamsdonk LW, Mol HG.Atypical myopathy (AM) in horses is caused by the plant toxin hypoglycin A, which in Europe typically is found in the sycamore maple tree (Acer pseudoplatanus). Owners are concerned about whether their horses are in danger if they graze near maple trees. Objective: To measure hypoglycin A in the most common maple tree species in the Netherlands, and to determine whether concentration of toxin is a predictor of AM in horses. Methods: A total of 278 samples of maple tree leaves, sprouts, and seeds were classified by species. Mean concentrations of hypoglycin A were compared for the type of sampl...
Questionnaire survey response rates in equine research.
Equine veterinary journal    January 29, 2016   Volume 48, Issue 2 138-139 doi: 10.1111/evj.12552
Christley RM.No abstract available
Welfare Conditions of Donkeys in Europe: Initial Outcomes from On-Farm Assessment.
Animals : an open access journal from MDPI    January 8, 2016   Volume 6, Issue 1 5 doi: 10.3390/ani6010005
Dai F, Dalla Costa E, Murray LM, Canali E, Minero M.This paper is a baseline study to present the initial outcomes of data collected in a sample of EU donkey farms using the AWIN welfare assessment protocol for donkeys, comprehensive of 22 valid, reliable and feasible animal-based indicators. A total of 20 donkey facilities (N = 12 in Italy and N = 8 in United Kingdom) were visited and 278 donkeys of different breed, aged 2-45 years, were assessed. Three assessors underwent a common training period to learn how to perform and score all the indicators included in the protocol. Data was collected using digitalized systems and downloaded to a data...
High-throughput analysis of horse sperms’ 3D swimming patterns using computational on-chip imaging.
Animal reproduction science    January 7, 2016   Volume 169 45-55 doi: 10.1016/j.anireprosci.2015.12.012
Su TW, Choi I, Feng J, Huang K, Ozcan A.Using a high-throughput optical tracking technique that is based on partially-coherent digital in-line holography, here we report a detailed analysis of the statistical behavior of horse sperms' three-dimensional (3D) swimming dynamics. This dual-color and dual-angle lensfree imaging platform enables us to track individual 3D trajectories of ∼1000 horse sperms at sub-micron level within a sample volume of ∼9μL at a frame rate of 143 frames per second (FPS) and collect thousands of sperm trajectories within a few hours for statistical analysis of their 3D dynamics. Using this high-throughp...
Effect of various testing conditions on results for a handheld reference point indentation instrument in horses.
American journal of veterinary research    December 29, 2015   Volume 77, Issue 1 39-49 doi: 10.2460/ajvr.77.1.39
Lescun TB, Hoffseth K, Yang HT, Hansma PK, Kopeikin HS, Chandrasekar S.To compare results obtained with a handheld reference point indentation instrument for bone material strength index (BMSi) measurements in the equine third metacarpal bone for various testing conditions. Methods: 24 third metacarpal bones. Methods: Third metacarpal bones from both forelimbs of 12 horses were obtained. The dorsal surface of each bone was divided into 6 testing regions. In vivo and ex vivo measurements of BMSi were obtained through the skin and on exposed bone, respectively, to determine effects of each testing condition. Difference plots were used to assess agreement between BM...
Targeted Metabolomics Approach To Detect the Misuse of Steroidal Aromatase Inhibitors in Equine Sports by Biomarker Profiling.
Analytical chemistry    December 15, 2015   Volume 88, Issue 1 764-772 doi: 10.1021/acs.analchem.5b03165
Chan GH, Ho EN, Leung DK, Wong KS, Wan TS.The use of anabolic androgenic steroids (AAS) is prohibited in both human and equine sports. The conventional approach in doping control testing for AAS (as well as other prohibited substances) is accomplished by the direct detection of target AAS or their characteristic metabolites in biological samples using hyphenated techniques such as gas chromatography or liquid chromatography coupled with mass spectrometry. Such an approach, however, falls short when dealing with unknown designer steroids where reference materials and their pharmacokinetics are not available. In addition, AASs with fast...
Analysis of breed effects on semen traits in light horse, warmblood, and draught horse breeds.
Theriogenology    December 10, 2015   Volume 85, Issue 8 1375-1381 doi: 10.1016/j.theriogenology.2015.11.030
Gottschalk M, Sieme H, Martinsson G, Distl O.In the present study, systematic effects on semen quality traits were investigated in 381 stallions representing 22 breeds. All stallions were used for AI either at the Lower Saxon National Stud Celle or the North Rhine-Westphalian National Stud Warendorf. A total of 71,078 fresh semen reports of the years 2001 to 2014 were edited for analysis of gel-free volume, sperm concentration, total number of sperm, progressive motility, and total number of progressively motile sperm. Breed differences were studied for warmblood and light horse breeds of both national studs (model I) and for warmblood b...
The use of electromyography interference pattern analysis to determine muscle force of the deep digital flexor muscle in healthy and laminitic horses.
The veterinary quarterly    November 26, 2015   Volume 36, Issue 1 10-15 doi: 10.1080/01652176.2015.1106116
Hardeman LC, van der Meij BR, Back W, van der Kolk JH, Wijnberg ID.In equine laminitis, the deep digital flexor muscle (DDFM) appears to have increased muscle force, but evidence-based confirmation is lacking. Objective: The purpose of this study was to test if the DDFM of laminitic equines has an increased muscle force detectable by needle electromyography interference pattern analysis (IPA). Methods: The control group included six Royal Dutch Sport horses, three Shetland ponies and one Welsh pony [10 healthy, sound adults weighing 411 ± 217 kg (mean ± SD) and aged 10 ± 5 years]. The laminitic group included three Royal Dutch Sport horses, one Friesian, o...
Modelling repeated competition records in genetic evaluations of Danish sport horses.
Journal of animal breeding and genetics = Zeitschrift fur Tierzuchtung und Zuchtungsbiologie    November 18, 2015   Volume 133, Issue 4 291-302 doi: 10.1111/jbg.12190
Jönsson L, Madsen P, Mark T.Genetic evaluations of sport performance typically consider competition records of ranking points in each competition, accumulated lifetime points or annual earnings. Repeated observations have the advantage of allowing for adjustment of effects associated with each competition such as rider experience, judge and competing horses, but also demands more computer capacity than single-trait records, which could prohibit multiple-trait evaluations. The aim of the study was to compare CPU times, estimated breeding values (EBVs), reliabilities and model prediction abilities when modelling repeated c...
A retrospective cohort study on fertility in the Norwegian Coldblooded trotter after artificial insemination with cooled, shipped versus fresh extended semen.
Acta veterinaria Scandinavica    November 14, 2015   Volume 57 77 doi: 10.1186/s13028-015-0161-8
Haadem CS, Nødtvedt A, Farstad W, Thomassen R.Pregnancy rates with cooled equine semen can be unsatisfactory and show great variation. Information about first cycle pregnancy rates and pregnancy rates per cycle are often lacking from publicly available records. This retrospective cohort study was performed to evaluate the fertility of the Norwegian Coldblooded trotter. The aim of the study was to compare the breeding results after insemination with fresh, extended with those of cooled, shipped semen among Norwegian Coldblooded trotter mares. First cycle pregnancy rate was the main parameter used to measure fertility. Stud-books were colle...
Prevalence of Streptococcus equi subsp. equi in horses and associated risk factors in the State of Rio Grande do Sul, Brazil.
Research in veterinary science    November 14, 2015   Volume 104 53-57 doi: 10.1016/j.rvsc.2015.11.009
Libardoni F, Machado G, Gressler LT, Kowalski AP, Diehl GN, dos Santos LC, Corbellini LG, de Vargas AC.The aim of this study was to estimate the prevalence of equine strangles and to identify associated risk factors for this disease through a cross-sectional study of nasal swabs. Nasal swabs (n=1010) from healthy equines (absence of nasal discharge, lymphadenopathy and cough) from 341 farms were plated on 5% blood agar; of these horses, 24 were identified as positive for Streptococcus equi through isolation, PCR and DNA sequencing. The estimated prevalence for individual animals was 2.3%, and for herds, it was 5.86%. Statistical analysis identified the following as associated risk factors: the ...
Equine grass sickness in Scotland: A case-control study of environmental geochemical risk factors.
Equine veterinary journal    October 30, 2015   Volume 48, Issue 6 779-785 doi: 10.1111/evj.12490
Wylie CE, Shaw DJ, Fordyce FM, Lilly A, Pirie RS, McGorum BC.We hypothesised that the apparent geographical distribution of equine grass sickness (EGS) is partly attributable to suboptimal levels of soil macro- and trace elements in fields where EGS occurs. If proven, altering levels of particular elements could be used to reduce the risk of EGS. Objective: To determine whether the geographical distribution of EGS cases in eastern Scotland is associated with the presence or absence of particular environmental chemical elements. Methods: Retrospective time-matched case-control study. Methods: This study used data for 455 geo-referenced EGS cases and 910 ...
Total nucleated cell and leukocyte differential counts in canine pleural and peritoneal fluid and equine synovial fluid samples: comparison of automated and manual methods.
Veterinary clinical pathology    October 26, 2015   Volume 44, Issue 4 570-579 doi: 10.1111/vcp.12298
Brudvig JM, Swenson CL.Rapid and precise measurement of total and differential nucleated cell counts is a crucial diagnostic component of cavitary and synovial fluid analyses. Objective: The objectives of this study included (1) evaluation of reliability and precision of canine and equine fluid total nucleated cell count (TNCC) determined by the benchtop Abaxis VetScan HM5, in comparison with the automated reference instruments ADVIA 120 and the scil Vet abc, respectively, and (2) comparison of automated with manual canine differential nucleated cell counts. Methods: The TNCC and differential counts in canine pleura...
Clinical Research Abstracts of the British Equine Veterinary Association Congress 2015.
Equine veterinary journal    September 17, 2015   Volume 47 Suppl 48 28 doi: 10.1111/evj.12486_63
Lotstra RJ, van den Broek J, Power T, Marr CM, Wijnberg ID.Atrial fibrillation is a common equine arrhythmia. Quinidine alone, or with digoxin are common treatments. Studies on outcome in Warmblood populations in which duration of the AF is often unknown are limited. Objective: To identify the factors that are associated with the success of full treatment cardioversion with oral medication, and establish whether there are differences in these factors between institutions. Methods: Retrospective case series using patient records of Equine University Clinic of Utrecht University and Rossdales Equine Hospital, Newmarket. Methods: Forty-nine horses treate...
Energetics of endurance exercise in young horses determined by nuclear magnetic resonance metabolomics.
Frontiers in physiology    July 15, 2015   Volume 6 198 doi: 10.3389/fphys.2015.00198
Luck MM, Le Moyec L, Barrey E, Triba MN, Bouchemal N, Savarin P, Robert C.Long-term endurance exercise severely affects metabolism in both human and animal athletes resulting in serious risk of metabolic disorders during or after competition. Young horses (up to 6 years old) can compete in races up to 90 km despite limited scientific knowledge of energetic metabolism responses to long distance exercise in these animals. The hypothesis of this study was that there would be a strong effect of endurance exercise on the metabolomic profiles of young horses and that the energetic metabolism response in young horses would be different from that of more experienced horses....
Sex ratio of equine offspring is affected by the ages of the mare and stallion.
Theriogenology    July 9, 2015   Volume 84, Issue 7 1238-1245 doi: 10.1016/j.theriogenology.2015.07.001
Santos MM, Maia LL, Nobre DM, Oliveira Neto JF, Garcia TR, Lage MC, de Melo MI, Viana WS, Palhares MS, da Silva Filho JM, Santos RL, Valle GR.The aim of this study was to determine the influence of parental age on the sex ratio of offspring in horses. Two trials were performed. In the first trial, the data from a randomly obtained population with a 1:1 sex ratio of 59,950 Mangalarga Marchador horses born in Brazil from 1990 to 2011 were analyzed. The sex ratios of the offspring were compared among groups according to the mare and the stallion ages (from 3 to 25 years). In the first step of the analysis, the mares and stallions were grouped according to age in 5-year intervals. In the second step, the groups were based on the parenta...
Genetic Variability of Show Jumping Attributes in Young Horses Commencing Competing.
Asian-Australasian journal of animal sciences    June 25, 2015   Volume 28, Issue 8 1090-1094 doi: 10.5713/ajas.14.0866
Próchniak T, Rozempolska-Rucińska I, Zięba G, Łukaszewicz M.The aim of the study was to select traits that may constitute a prospective criterion for breeding value prediction of young horses. The results of 1,232 starts of 894 four-, five-, six-, and seven-year-old horses, obtained during jumping championships for young horses which had not been evaluated in, alternative to championships, training centres were analyed. Nine traits were chosen of those recorded: ranking in the championship, elimination (y/n), conformation, rating of style on day one, two, and three, and penalty points on day one, two, and three of a championship. (Co)variance component...
Twenty years later: a single-centre, repeat retrospective analysis of equine perioperative mortality and investigation of recovery quality.
Veterinary anaesthesia and analgesia    June 15, 2015   Volume 43, Issue 2 171-178 doi: 10.1111/vaa.12285
Dugdale AH, Obhrai J, Cripps PJ.To determine the mortality rates associated with equine anaesthesia for elective and emergency (colic and non-colic) cases in one equine, university teaching hospital and to investigate the effect of several horse- and anaesthetic-related variables on anaesthetic recovery quality. Methods: Retrospective data analysis. Unassigned: In total, 1416 horses undergoing anaesthesia between May 2010 and December 2013. Methods: Patient information and details of the anaesthetic, recovery period and immediate complications were extracted from an archiving database. Statistical evaluation of factors affec...
Potential role of maternal lineage in the thoroughbred breeding strategy.
Reproduction, fertility, and development    May 5, 2015   doi: 10.1071/RD15063
Lin X, Zhou S, Wen L, Davie A, Yao X, Liu W, Zhang Y.Many studies have focused on identifying the genes or single nucleotide polymorphisms associated with the athletic ability of thoroughbreds, but few have considered differences in maternal and paternal heritability of athletic ability. Herein, we report on our association study of career race performances of 675 Australian thoroughbreds with their pedigrees. Racing performance data (prize money per start) were collected from the Bloodhound database. The performance of all horses was categorised as either poor or elite athletic achievement. Then, 675 foals were divided by their parents' perform...
A mechanostatistical approach to cortical bone remodelling: an equine model.
Biomechanics and modeling in mechanobiology    April 11, 2015   Volume 15, Issue 1 29-42 doi: 10.1007/s10237-015-0669-x
Wang X, Thomas CD, Clement JG, Das R, Davies H, Fernandez JW.In this study, the development of a mechanostatistical model of three-dimensional cortical bone remodelling informed with in vivo equine data is presented. The equine model was chosen as it is highly translational to the human condition due to similar Haversian systems, availability of in vivo bone strain and biomarker data, and furthermore, equine models are recommended by the US Federal Drugs Administration for comparative joint research. The model was derived from micro-computed tomography imaged specimens taken from the equine third metacarpal bone, and the Frost-based 'mechanostat' was in...
DMRT3 is associated with gait type in Mangalarga Marchador horses, but does not control gait ability.
Animal genetics    February 18, 2015   Volume 46, Issue 2 213-215 doi: 10.1111/age.12273
Patterson L, Staiger EA, Brooks SA.The Mangalarga Marchador (MM) is a Brazilian horse breed known for a uniquely smooth gait. A recent publication described a mutation in the DMRT3 gene that the authors claim controls the ability to perform lateral patterned gaits (Andersson et al. 2012). We tested 81 MM samples for the DMRT3 mutation using extracted DNA from hair bulbs using a novel RFLP. Horses were phenotypically categorized by their gait type (batida or picada), as recorded by the Brazilian Mangalarga Marchador Breeders Association (ABCCMM). Statistical analysis using the plink toolset (Purcell, 2007) revealed significant a...
Statistical guidelines for Equine Veterinary Journal.
Equine veterinary journal    February 4, 2015   Volume 47, Issue 2 131-132 doi: 10.1111/evj.12400
Christley R.No abstract available
The use of novel phenotyping methods for validation of equine conformation scoring results.
Animal : an international journal of animal bioscience    January 13, 2015   Volume 9, Issue 6 928-937 doi: 10.1017/S1751731114003309
Druml T, Dobretsberger M, Brem G.In this experiment, which is based on a cohort of 44 Lipizzan mares from the Austrian state stud farm of Piber, we present new statistical techniques for the analysis of shape and equine conformation using image data. In addition, we examined which strategies and procedures of image processing techniques led to a successful interpretation of the traits implemented in horse breeding programs. A total of 246 two-dimensional anatomical and somatometric landmarks were digitized from standardized photographs, and the variation of shape has been analyzed by the use of generalized orthogonal least-sq...
Using Bayes’ rule to define the value of evidence from syndromic surveillance.
PloS one    November 3, 2014   Volume 9, Issue 11 e111335 doi: 10.1371/journal.pone.0111335
Andersson MG, Faverjon C, Vial F, Legrand L, Leblond A.In this work we propose the adoption of a statistical framework used in the evaluation of forensic evidence as a tool for evaluating and presenting circumstantial "evidence" of a disease outbreak from syndromic surveillance. The basic idea is to exploit the predicted distributions of reported cases to calculate the ratio of the likelihood of observing n cases given an ongoing outbreak over the likelihood of observing n cases given no outbreak. The likelihood ratio defines the Value of Evidence (V). Using Bayes' rule, the prior odds for an ongoing outbreak are multiplied by V to obtain the post...
Significance of ossificated ungular cartilages regarding the performance of cold-blooded trotters.
Acta veterinaria Scandinavica    October 25, 2014   Volume 56, Issue 1 74 doi: 10.1186/s13028-014-0074-y
Hedenström UO, Wattle OS.Ossification of the ungular cartilages (OUC) in the foot of horses has been studied for more than 100 years. There is a high heritability of this condition but its clinical relevance has remained questionable. Nevertheless, modern equine orthopedic literature ranks OUC as one of top 10 causes of lameness in cold-blooded trotters and stallions of these breeds are excluded from breeding if they have more than mild levels of side bones. Cold-blooded trotters have been used for racing for many decades and official sports data have been available since 1923. A decreased performance is often the onl...
Validation study of an interpolation method for calculating whole lung volumes and masses from reduced numbers of CT-images in ponies.
Veterinary journal (London, England : 1997)    October 22, 2014   Volume 202, Issue 3 603-607 doi: 10.1016/j.tvjl.2014.10.019
Reich H, Moens Y, Braun C, Kneissl S, Noreikat K, Reske A.Quantitative computer tomographic analysis (qCTA) is an accurate but time intensive method used to quantify volume, mass and aeration of the lungs. The aim of this study was to validate a time efficient interpolation technique for application of qCTA in ponies. Forty-one thoracic computer tomographic (CT) scans obtained from eight anaesthetised ponies positioned in dorsal recumbency were included. Total lung volume and mass and their distribution into four compartments (non-aerated, poorly aerated, normally aerated and hyperaerated; defined based on the attenuation in Hounsfield Units) were de...
Occurrence of Incisional Complications After Closure of Equine Celiotomies With USP 7 Polydioxanone.
Veterinary surgery : VS    September 17, 2014   Volume 44, Issue 4 521-526 doi: 10.1111/j.1532-950X.2014.12275.x
Anderson SL, Devick I, Bracamonte JL, Hendrick S, Barber SM, Carmalt JL, Wilson DG.To report the occurrence of, and risk factors associated with, incisional complications of equine ventral median celiotomies closed with USP 7 polydioxanone (7PD). Methods: Retrospective case series. Methods: Horses (n = 73; 75 celiotomies). Methods: Medical records (2000-2010) were retrieved and reviewed for horses that had a ventral celiotomy closed with 7PD and survived ≥14 days. Follow-up (>1 year) was obtained from the medical record, owner, or referring veterinarian. Independent variables were selected based on risk factors identified in previous studies, perceived clinical relevan...
Can high-resolution peripheral quantitative computed tomography imaging of subchondral and cortical bone predict condylar fracture in Thoroughbred racehorses?
Equine veterinary journal    September 5, 2014   Volume 47, Issue 4 428-432 doi: 10.1111/evj.12312
Trope GD, Ghasem-Zadeh A, Anderson GA, Mackie EJ, Whitton RC.High-resolution 3D imaging may improve the prediction and/or early identification of condylar fractures of the distal metacarpus/tarsus and reduce the frequency of breakdown injury in racehorses. Objective: To test the hypotheses that horses suffering condylar fractures have higher bone volume fraction (BV/TV) of the distal metacarpal epiphysis, greater subchondral bone thickness at the fracture site and higher second moment of inertia in the metacarpal midshaft as identified with high-resolution 3D imaging. Methods: Cross-sectional study using cadaver material. Methods: Thoroughbreds that die...
Comparison of equine articular cartilage thickness in various joints.
Connective tissue research    August 26, 2014   Volume 55, Issue 5-6 339-347 doi: 10.3109/03008207.2014.949698
Lee H, Kirkland WG, Whitmore RN, Theis KM, Young HE, Richardson AJ, Jackson RL, Hanson RR.Thicknesses of fresh equine articular cartilage surfaces from the fetlock, carpal and stifle joints were measured employing a needle probe test. Methods: Eighty-seven samples used in measurement were cultivated from fetlock, carpal and stifle joints of 12 deceased within 4 h of death. After approximately three minutes of exposure to air during dissection, all cartilage samples were preserved in a saline solution to keep the articular cartilage hydrated for testing. The thickness was measured on five different spots on the same sample. The thicknesses of the fetlock, carpus and stifle were co...
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