Analyze Diet

Topic:Body Weight

Body weight in horses is a fundamental parameter that influences various aspects of equine management and health. It is a measure of the horse's mass, often assessed using scales or estimated through body condition scoring and weight tapes. Body weight is integral for determining appropriate feed rations, medication dosages, and workload capacities. Variations in body weight can indicate changes in health status, nutritional adequacy, or fitness levels. This page compiles peer-reviewed research studies and scholarly articles that explore the methodologies for assessing body weight, its implications on equine health and performance, and its role in management practices.
Effects of Dietary Zn/Se and α-Tocopherol Supplementation on Metabolic Milieu, Haemogram and Semen Traits of Breeding Stallions.
Biological trace element research    October 23, 2020   Volume 199, Issue 9 3287-3296 doi: 10.1007/s12011-020-02447-7
Cappai MG, Taras A, Cossu I, Cherchi R, Dimauro C, Accioni F, Boatto G, Deroma M, Spanu E, Gatta D, Dall'Aglio C, Pinna W.Trace element status and metabolic milieu are sometimes overlooked in common veterinary clinical practice across animal species. The evaluation of requirements of trace elements, in fact, may be useful to prevent the perturbation of tissue-specific metabolic impair. In particular, essential trace elements in the diet play key roles within sub-cellular metabolic patterns with macro effects at the systemic level, like blood cell stability and semen quality. This effect was studied in breeding stallions, in which semen quality and haemogram are important for reproduction. A case-control feeding t...
Does size matter? Examining the possible mechanisms of multi-stallion groups in horse societies.
Behavioural processes    October 20, 2020   Volume 181 104277 doi: 10.1016/j.beproc.2020.104277
Pinto P, Hirata S.In some feral horse populations, adult females are either associated with a single male or multiple males (stallions). However, little is known on why such groups with more than one male exist, considering that stallions fight to monopolize females. Body size is often an important determinant of male fighting ability and/or dominance rank and, consequently, reproductive success. Stallions may, therefore, vary in "quality" which could be a differentiating factor between single-stallion and multi-stallion males. We observed feral Garrano horses in Northern Portugal and examined the relationship ...
Serum symmetric dimethylarginine concentration in healthy horses and horses with acute kidney injury.
BMC veterinary research    October 20, 2020   Volume 16, Issue 1 396 doi: 10.1186/s12917-020-02621-y
Siwinska N, Zak A, Slowikowska M, Niedzwiedz A, Paslawska U.There are limited options to diagnose acute kidney injury (AKI) in horses. Symmetric dimethylarginine (SDMA) is routinely used in human and small animal medicine. The aim of this study was to assess serum SDMA concentrations in healthy horses and horses with AKI. The objective of this study was to evaluate the association of: 1) age, 2) sex, 3) body weight and 4) serum creatinine and urea levels on serum SDMA concentrations. Fifty-three healthy horses, including 17 foals (2-6 months of age) and 36 adult horses (3-29 years of age), and 23 horses with AKI were included in the study based on ...
Relationship between live body condition score and carcass fat measures in equine.
Translational animal science    October 1, 2020   Volume 4, Issue 4 txaa179 doi: 10.1093/tas/txaa179
Baker LA, Burrows AM, Nonella KJ, Pipkin JL, Holmes LD, McEvers TJ, Tennant TC, Tisdale ZM, Voyles AH, Lawrence TE.Relationships between live body condition score (BCS) and carcass fat depots have not been well established in equine. Our study was designed to quantify the relationship between BCS and fat depot measurements from equine carcasses. Live horses ( = 429) were evaluated immediately prior to immobilization at a commercial equine processor. Horses were independently assigned a BCS by a panel of three trained evaluators; BCS was evaluated by visual appraisal and manual palpation of the neck, withers, back, ribs, behind the shoulder, and tailhead. Median BCS frequencies were: 3.0 ( = 9), 4.0 ( = 43)...
Evaluation of the Accuracy of Horse Body Weight Estimation Methods.
Animals : an open access journal from MDPI    September 26, 2020   Volume 10, Issue 10 1750 doi: 10.3390/ani10101750
Górniak W, Wieliczko M, Soroko M, Korczyński M.Methods of estimating horse body weight using mathematical formulae have better accuracy than methods of reading body weight from measuring tape. The aim of the study was to evaluate established formulae for estimating horse body weight from data gathered using measurement tape. The research was conducted in a group of 299 adult horses and ponies of selected breeds: ponies ( = 58), Polish Noble Half Breed ( = 150), Silesian Breed ( = 23), Wielkopolski Breed ( = 52), and Thoroughbred ( = 16). Body measurements were performed on each horse using a measuring stick and tape. The actual body weight...
High or Low Body Fat Deposition in the Presence of a Normal Oral Sugar Test is Not Associated With Postthaw Semen Parameters in Stallions.
Journal of equine veterinary science    September 24, 2020   Volume 95 103271 doi: 10.1016/j.jevs.2020.103271
Novello G, Segabinazzi LGTM, Lisboa FP, Canuto LE, Freitas-Dell'Aqua CP, Dell'Aqua JA, Canisso IF.This study compared the postthaw semen parameters of stallions with high and low body condition score (BCS) and evaluated associations between body morphometric parameters and postthaw semen parameters. Twenty stallions were split into Low BCS (BCS<7, n = 11) and High BCS (BCS ≥7, n = 9) groups, and underwent a complete morphometric analysis (e.g., neck scores and circumference, crest neck height, body weight, and height), and subcutaneous body fat thickness (SFT) at the tail head, withers, shoulders, and retroperitoneal space. A fasted oral sugar test (OST) was conducted on all stallio...
Gymnastic Training of Hippotherapy Horses Benefits Gait Quality When Ridden by Riders with Different Body Weights.
Journal of equine veterinary science    September 12, 2020   Volume 94 103248 doi: 10.1016/j.jevs.2020.103248
de Oliveira K, Clayton HM, Dos Santos Harada É.The objective was to evaluate the effects of gymnastic training on stride characteristics of walk and trot in therapy horses carrying riders of different weights. Eighteen horses used for therapeutic riding 5 days/week were randomly divided into 2 groups. Nine horses performed gymnastic (GYM) exercises after therapeutic riding on 4 days/week for 3 months, 9 horses did no additional exercises (SED). On days 0 and 90, an inertial sensor mounted to the girth on the ventral midline was used to evaluate stride characteristics when horses were ridden at walk (1.3 m/second) and trot (3.0 m/secon...
Dry Matter Intake, Body Weight, and Body Condition Scores of Blanketed and Nonblanketed Horses in the Upper Midwest.
Journal of equine veterinary science    August 29, 2020   Volume 94 103239 doi: 10.1016/j.jevs.2020.103239
DeBoer M, Konop A, Fisher B, Martinson K.Thermoregulation is an energy-expensive process, which can be mitigated by blanketing horses in cold climates, potentially preventing weight loss or leading to decreased feed intake. The objective of this study was to evaluate feed intake, body weight (BW), and body condition scores (BCSs) in blanketed and nonblanketed horses. In October 2019, 16 mature adult horses were blocked by breed and BCS and randomly assigned to a blanketed (n = 8) or nonblanketed (n = 8) treatment; blankets were placed. Data were collected in December 2019 and January 2020 in River Falls, Wisconsin. During the study...
Pathways regulating equine skeletal muscle protein synthesis respond in a dose-dependent manner to graded levels of protein intake.
Journal of animal science    August 25, 2020   Volume 98, Issue 9 doi: 10.1093/jas/skaa268
Loos CMM, McLeod KR, Stratton SC, van Doorn DA, Kalmar ID, Vanzant ES, Urschel KL.Activation of the mechanistic target of rapamycin (mTOR)-controlled anabolic signaling pathways in skeletal muscle of rodents and humans is responsive to the level of dietary protein supply, with maximal activation and rates of protein synthesis achieved with 0.2 to 0.4 g protein/kg body weight (BW). In horses, few data are available on the required level of dietary protein to maximize protein synthesis for maintenance and growth of skeletal muscle. To evaluate the effect of dietary protein level on muscle mTOR pathway activation, five mares received different amounts of a protein supplement t...
Evaluation of an oral joint supplement on gait kinematics and biomarkers of cartilage metabolism and inflammation in mature riding horses.
Translational animal science    August 24, 2020   Volume 4, Issue 3 txaa150 doi: 10.1093/tas/txaa150
Much ML, Leatherwood JL, Martinez RE, Silvers BL, Basta CF, Gray LF, Bradbery AN.Twenty stock-type horses (589 ± 126 kg BW; 13 ± 8 yr) were used in a completely randomized design for 28-d to evaluate the impact of a joint supplement on gait kinematics, inflammation, and cartilage metabolism. Horses were stratified by age, sex, body weight (BW), and initial lameness scores and were randomly assigned to one of two dietary treatments consisting of either a 100-g placebo top-dressed daily to 0.6% BW (as-fed) commercial concentrate (CON; n = 10; SafeChoice Original, Cargill, Inc.), or an oral joint supplement (SmartPak Equine LLC) containing glucosamine, chondroitin sulfate, ...
Variation of Hematochemical Profile and Vitamin E Status in Feral Giara Horses From Free Grazing in the Wild to Hay Feeding During Captivity.
Journal of equine veterinary science    August 7, 2020   Volume 94 103220 doi: 10.1016/j.jevs.2020.103220
Cappai MG, Pudda F, Wolf P, Accioni F, Boatto G, Pinna W.Wildlife protection and management are important priorities for landscape identity and biodiversity preservation. Feeding practices of fauna confined in facilities during temporary captivity are fundamental to support animal health and natural behavior. Appropriate provision of feedstuffs appears to be necessary to support the best practices in respect of animal species-specific natural diet. This investigation explored the variation of the metabolic profile by means of selected metabolite and respective circulating levels in a group feral Giara horses undergoing the change of the diet, moving...
Clinical insights: Equine obesity.
Equine veterinary journal    August 5, 2020   Volume 52, Issue 5 635-638 doi: 10.1111/evj.13307
Knowles EJ, Grieve L.No abstract available
Evaluation of jennies’ colostrum: IgG concentrations and absorption in the donkey foals. A preliminary study.
Heliyon    August 3, 2020   Volume 6, Issue 8 e04598 doi: 10.1016/j.heliyon.2020.e04598
Immunoglobulin type G (IgG) concentration both in jennies' colostrum and in serum of donkey foals are mostly unknown in the first 24 h after delivery. The aims of the present study were to evaluate the IgG concentrations of colostrum during the first 24 h of lactation of Amiata jennies, the absorption of colostrum and the weekly body weight gain of the donkey foals. IgG concentrations were assessed in the jennies' colostrum and in the serum of donkey foals. Colostrum was collected in 9 jennies ready after delivery, and at 6, 12, 24 h after foaling from both halves. Serum was collected at the s...
Effects of aquatic conditioning on cartilage and bone metabolism in young horses.
Journal of animal science    July 28, 2020   Volume 98, Issue 8 skaa239 doi: 10.1093/jas/skaa239
Silvers BL, Leatherwood JL, Arnold CE, Nielsen BD, Huseman CJ, Dominguez BJ, Glass KG, Martinez RE, Much ML, Bradbery AN.While beneficial in rehabilitation, aquatic exercise effects on cartilage and bone metabolism in young, healthy horses has not been well described. Therefore, 30 Quarter Horse yearlings (343 ± 28 kg; 496 ± 12 d of age) were stratified by age, body weight (BW), and sex and randomly assigned to 1 of 3 treatments for 140-d to evaluate effects of aquatic, dry, or no exercise on bone and cartilage metabolism in young horses transitioning to an advanced workload. Treatments included nonexercise control (CON; n = 10), dry treadmill (DRY; n = 10), or aquatic treadmill exercise (H2O; n = 10; water: 6...
Growth Rates of Thoroughbred Foals and In Vitro Gut Health Parameters When Fed a Cereal or an All-Fiber Creep Feed.
Journal of equine veterinary science    July 15, 2020   Volume 93 103191 doi: 10.1016/j.jevs.2020.103191
Moore-Colyer M, Tuthill P, Bannister I, Daniels S.Traditions and the economic advantage when producing big athletic Thoroughbred (TB) yearlings for sale encourage owners to feed high levels of cereals. Such diets can predispose gastric ulceration and developmental orthopedic diseases, impacting negatively on future careers. This study aimed to (1) determine if an all-fiber creep feed (total mixed fiber ration [TMFR] would sustain equal growth rates in TB foals versus a cereal-based stud cube (SC) and (2) compare pH and lactate produced by SC and TMFR in in vitro foregut and hindgut conditions. Eight TB foals in matched-pairs were fed SC or T...
The effect of diet-induced obesity and pasture on blood pressure and serum cortisol in Standardbred mares.
Equine veterinary journal    July 4, 2020   Volume 53, Issue 3 542-548 doi: 10.1111/evj.13294
Nostell K, Lindåse S, Winqvist E, Bröjer J.Obesity is associated with insulin resistance, vascular dysfunction and altered cortisol metabolism both in humans and in horses. Objective: Evaluate the effect of weight gain induced by a haylage diet low in nonstructural carbohydrates (NSC) on insulin sensitivity, blood pressure and serum cortisol concentrations. Methods: In vivo experiment. Methods: Nine adult Standardbred mares fed a fat supplemented haylage diet at 250% of the horses' daily metabolisable energy requirements for 22 weeks. Horses were then turned out on pasture for 4 weeks. Insulin sensitivity (SI ) was measured before an...
Effects of 27 mo of rotational vs. continuous grazing on horse and pasture condition.
Translational animal science    June 20, 2020   Volume 4, Issue 3 txaa084 doi: 10.1093/tas/txaa084
Williams CA, Kenny LB, Weinert JR, Sullivan K, Meyer W, Robson MG.The objective of this study was to determine whether rotational grazing generates horse, pasture, or cost benefits over continuous grazing. The study established two replicates (1.57 ha each) of rotational (R; four grazing sections and a stress lot per replicate, where horses were fed a moderate quality grass hay at 2% of body weight when not grazing) and continuous (C) grazing systems (treatments). Twelve Standardbred mares were grazed for an overall stocking rate of 0.52 ha/horse ( = 3 in each pasture). Recommended management practices for each grazing system were followed for 27 mo includin...
Twenty-four-hour rhythm patterns of plasma melatonin in short-day and long-day breeders maintained under natural environmental conditions.
Chronobiology international    June 2, 2020   Volume 37, Issue 7 974-979 doi: 10.1080/07420528.2020.1772808
Giannetto C, Carcangiu V, Luridiana S, Parmeggiani A, Piccione G.Photoperiodic treatments have been of practical interest in controlling seasonal reproduction in sheep, goats and horses. Melatonin is the principal mediator of the environmental photoperiodic message. To investigate the intra- and inter-subject variability of melatonin 24 h rhythm, ten female Italian Saddle horses (8-10 yrs old, mean body weight 525 ± 30 kg), ten female Sarda breed sheep (2-3 yrs old, mean body weight 40.5 ± 2.8 kg) and ten female Sarda breed goats (3-4 yrs old, mean body weight 38.9 ± 4.1 kg), housed individually in a 4 × 4 m soundproof box equipped with 50 Ã...
Weight loss is linearly associated with a reduction of the insulin response to an oral glucose test in Icelandic horses.
BMC veterinary research    May 24, 2020   Volume 16, Issue 1 151 doi: 10.1186/s12917-020-02356-w
Delarocque J, Frers F, Huber K, Feige K, Warnken T.Insulin dysregulation (ID) goes along with lasting or transient hyperinsulinemia able to trigger equine laminitis, a painful and crippling foot condition. Promoting weight loss through dietary changes and physical activity is currently the main option to prevent this disease. This study aimed at describing the relationship between weight variations and the level of ID as determined by oral glucose tests (OGT). Therefore, the insulin response of 19 Icelandic horses to repeated OGTs was retrospectively analysed considering the variations in their body weight. Results: There was a strong linear r...
Donkey Feeding During Maintenance, Pregnancy, and Lactation: Effects on Body Weight, Milk Production, and Foal Growth.
Journal of equine veterinary science    May 22, 2020   Volume 91 103131 doi: 10.1016/j.jevs.2020.103131
Salari F, Licitra R, Altomonte I, Martini M.We evaluated the daily intake in donkeys during maintenance, late pregnancy, and early lactation. The growth curves of the foals in the first eight weeks of life and the milk production in lactating jennies were also investigated. Donkeys were separated into two groups: seven pregnant jennies (group 1: pregnant/lactating) and seven nonpregnant, nonlactating jennies (group 2). The groups were fed two different diets each. The feeding period for group 1 covered eight weeks before parturition and eight weeks postpartum. Group 2 was managed during the same time period (16 weeks). Diet 1 consisted...
Alterations of serum vitamin E and vitamin A concentrations of ponies and horses during experimentally induced obesity.
Journal of animal physiology and animal nutrition    May 14, 2020   Volume 104, Issue 5 1501-1508 doi: 10.1111/jpn.13385
Schedlbauer C, Blaue D, Raila J, Vervuert I.Vitamin A, vitamin E and retinol-binding protein 4 (RBP4) are a focus of current obesity research in humans. The impact of body weight (BW) gain on fat-soluble vitamins and its associated parameters in equines has not been previously reported. Ten Shetland ponies and 9 Warmblood horses, all adult geldings, non-obese and healthy, were fed an excessive energy diet for 20 months to induce BW gain. Serum α-tocopherol (vitamin E), retinol (vitamin A), retinol-binding protein 4 (RBP4) and retinol/RBP4 ratio were analysed before BW gain induction and at six timepoints during the BW gaining period. ...
The effect of crude protein content of the diet on renal energy losses in horses.
Journal of animal physiology and animal nutrition    May 14, 2020   Volume 104, Issue 5 1494-1500 doi: 10.1111/jpn.13377
Kuchler M, Zeyner A, Susenbeth A, Kienzle E.Renal energy losses of horses are high in comparison with other species. In the present study, more data were obtained on this parameter to improve predictive equations for renal energy losses. Four adult ponies (247-344 kg body weight [BW]) were fed with eight different diets based on first cut hay, second cut hay, early first cut fresh grass, late cut herbs-grass mix, early cut clover-grass mix, sugar beet pulp, rice bran and straw. Feed intake was measured, and urine and faeces were quantitatively collected for 3 × 12 hr at daytime and afterwards 3 × 12 hr at nighttime. Feed was an...
Effect of long-term overfeeding of a high-energy diet on glucose tolerance in Shetland pony mares.
Journal of veterinary internal medicine    May 6, 2020   Volume 34, Issue 3 1339-1349 doi: 10.1111/jvim.15788
d' Fonseca NMM, Gibson CME, van Doorn DA, de Ruijter-Villani M, Stout TAE, Roelfsema E.Overfeeding is associated with obesity and insulin dysregulation (ID), which are both risk factors for equine metabolic syndrome. How chronic overfeeding affects development of these factors is poorly understood. Objective: To examine the influence of long-term high-energy diet provision on body condition and ID. Methods: Eleven Shetland pony mares. Methods: In a 3-phase study, the high-energy group (n = 7) was fed 200% of net energy (NE) requirements (hay; concentrate: 36% sugar and starch, 13% fat) for 24 weeks, followed by 17 weeks hay-only feeding before resuming the high-energy diet (...
Quantification of left atrial wall motion in healthy horses using two-dimensional speckle tracking.
Journal of veterinary cardiology : the official journal of the European Society of Veterinary Cardiology    May 4, 2020   Volume 30 32-43 doi: 10.1016/j.jvc.2020.04.003
Eberhardt C, Mitchell KJ, Schwarzwald CC.The mechanical function of the left atrium (LA) plays a pivotal role in modulation of left ventricular filling. Assessment of LA function might be a clinically useful prognostic tool for horses with mitral regurgitation or atrial fibrillation. However, the most accurate, reliable, and clinically useful methods to assess LA myocardial function are yet to be determined. The objective of this study was to describe the methods for quantification of LA wall motion using two-dimensional speckle tracking (2DST) echocardiography in healthy Warmblood horses to quantify measurement variability, to calcu...
Two-dimensional, M-mode and left atrial velocity echocardiographic measurements in healthy Norwegian-Swedish Coldblooded Trotters.
Journal of veterinary cardiology : the official journal of the European Society of Veterinary Cardiology    May 4, 2020   Volume 29 60-73 doi: 10.1016/j.jvc.2020.04.006
Fintl C, Krontveit R, Risberg Å, Hanche-Olsen S, Slack J.The Norwegian-Swedish Coldblooded Trotter (NSCT) is an important breed in Norwegian and Swedish harness racing. In spite of this, little is known about its cardiac measurements and factors that may influence these. The aims of this study were to establish reference intervals (RI) for a series of conventional echocardiographic variables, primarily of the left heart, in NSCTs in race training and to evaluate any effects age, gender, estimated body weight (EBW), number of starts and racing performance may have on these. Methods: Sixty-three apparently healthy NSCTs in race training. Methods: Obse...
Dietary supplementation of micro-encapsulated sodium butyrate in healthy horses: effect on gut histology and immunohistochemistry parameters.
BMC veterinary research    April 28, 2020   Volume 16, Issue 1 121 doi: 10.1186/s12917-020-02332-4
Wambacq WA, van Doorn DA, Rovers-Paap PM, Ducatelle R, Vlaminck L, Lourenço M, Hesta M.As colic and intestinal disorders are a major concern in horses, the aim of the present study was to investigate the effect of dietary supplementation of butyrate, known to have a diverse array of beneficial effects on intestinal health. The effect of micro-encapsulated sodium butyrate supplementation on gut histology and immunohistochemistry parameters was studied in 14 healthy warmblood horses destined for slaughter in two separate periods. Horses were fed a low fiber - high starch diet, designed to induce subsequent starch overflow in the large intestine, aiming to create a mild challenge f...
The influence of equine body weight gain on inflammatory cytokine expressions of adipose tissue in response to endotoxin challenge.
Acta veterinaria Scandinavica    April 22, 2020   Volume 62, Issue 1 17 doi: 10.1186/s13028-020-00515-5
Blaue D, Schedlbauer C, Starzonek J, Gittel C, Brehm W, Blüher M, Pfeffer M, Vervuert I.Human obesity is linked with systemic inflammation. However, it is still controversial if equines produce more inflammatory cytokines with increasing body weight and if the production of those show breed type specific patterns. The main objective of this study was to determine if diet induced obesity is associated with increased inflammatory signatures in adipose tissue of equines and if a breed predisposition exists between ponies and horses. Additionally, we aimed to identify adipose tissue depot differences in inflammatory cytokine expression. Nineteen healthy, non-overweight and metabolica...
Early predictors of female lifetime reproductive success in a solitary hibernator: evidence for “silver spoon” effect.
Oecologia    April 21, 2020   Volume 193, Issue 1 77-87 doi: 10.1007/s00442-020-04649-1
Vasilieva NA, Tchabovsky AV.Fitness consequences of early-life conditions remain unclear and poorly studied in mammals. Based on long-term observations of yellow ground squirrels (Spermophilus fulvus), we identified early determinants of female fitness by analyzing the effects of early-life individual and environmental characteristics (weaning weight, weight gain rate, date of natal emergence, natal litter size, location of the natal burrow, local density of juveniles, population density and precipitation in the post-weaning period) on lifetime reproductive success (LRS). We found high variation and right-skewed distribu...
In Vitro Gas Production from Batch Cultures of Stomach and Hindgut Digesta of Horses Adapted to a Prebiotic Dose of Fructooligosaccharides and Inulin.
Journal of equine veterinary science    April 9, 2020   Volume 90 103020 doi: 10.1016/j.jevs.2020.103020
Bachmann M, Glatter M, Bochnia M, Wensch-Dorendorf M, Greef JM, Breves G, Zeyner A.Fructooligosaccharides (FOS) and inulin may modulate hindgut fermentation. It was tested if digesta batch cultures taken from horses adapted to FOS and inulin show different fermentation compared with such taken from nonsupplemented horses. Six horses received 0.15 g FOS and inulin/kg body weight/d via Jerusalem artichoke meal (JAM) upon a hay-based diet; six horses received corncob meal without grains (CMG) as placebo. The horses were euthanized after 20 days. Digesta samples were taken from stomach, cecum, ventral colon ascendens (VCA), and colon transversum (CT). Digesta batch cultures we...
Effect of feed deprivation on daily water consumption in healthy horses.
Equine veterinary journal    March 30, 2020   Volume 53, Issue 1 117-124 doi: 10.1111/evj.13259
Freeman DE, Mooney A, Giguère S, Claire J, Evetts C, Diskant P.Measurements of water consumed by fed healthy horses might not apply to horses that are unwilling or unable to drink or are not fed for any reason. Objective: To examine the effects of feed deprivation on voluntary water consumption compared with fed conditions. Methods: In vivo experiment. Methods: Eight healthy adult Thoroughbred geldings were used in a randomised crossover design so that each horse served as its own control for fed vs feed-deprived conditions. Water intake, bodyweight, physical findings and vital signs were measured during 4 days of feeding and 4 days of feed deprivation....
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