Analyze Diet

Topic:Forage

Forage is a primary component of the equine diet, consisting mainly of grasses, hay, and legumes that provide essential nutrients and fiber. It plays a significant role in maintaining digestive health and supporting the overall well-being of horses. The nutritional composition of forage can vary based on factors such as plant species, maturity at harvest, and environmental conditions. Proper management and selection of forage are important for meeting the dietary needs of horses and preventing issues such as colic or laminitis. This page compiles peer-reviewed research studies and scholarly articles that explore the nutritional value, management practices, and impact of forage on equine health and performance.
Time-activity budget in horses and ponies: A systematic review and meta-analysis on feeding dynamics and management implications.
Journal of equine veterinary science    September 3, 2025   Volume 154 105684 doi: 10.1016/j.jevs.2025.105684
Lamanna M, Buonaiuto G, Colleluori R, Raspa F, Valle E, Cavallini D.The time-activity budget is a key indicator of animal welfare. This meta-analysis integrated 14 studies (1979-2020) with 364 horses under wild, natural-living, and stabled conditions to evaluate feeding, resting, standing, and locomotion. Objective: The study aimed to categorize daily behavioural patterns in equines, with emphasis on feeding behaviour. Methods: Fixed and random effects models, forest plots, and ANOVA assessed the influence of management, sociality, sex, age, body size, and feeding type. Results: Feeding was greater in free-ranging (56 %) than stabled horses (38 %; p < 0.000...
Risk factors associated with gastric disease prevalence in extensively kept horses in Iceland evaluated four times in a calendar year.
Equine veterinary journal    September 1, 2025   doi: 10.1111/evj.70072
Luthersson N, Harris PA, Parkin T, Þorgrímsdóttir ÚÝ, Bennet ED.It is unknown whether the high prevalence of Equine Squamous (ESGD) and Equine Glandular (EGGD) Gastric Disease in extensively grazed Icelandic horses in the autumn/winter is seasonally driven. Objective: To determine the prevalence of, and risk factors for, gastroscopically significant ESGD (ESGD:score of ≥2/4); gastroscopically severe ESGD (ESGD:score of ≥3/4) and gastroscopically significant EGGD (EGGD:score of ≥1/2) in extensively pasture-managed Icelandic horses at four timepoints. Methods: Prospective longitudinal cohort. Methods: Gastroscopy was undertaken in 80 Icelandic horses (...
Microbiome and Dental Changes in Horses Fed a High Soluble Carbohydrate Diet.
Animals : an open access journal from MDPI    August 29, 2025   Volume 15, Issue 17 2547 doi: 10.3390/ani15172547
Lacerenza MD, Arantes JA, Reginato GM, Finardi GLF, Marchi PH, Vendramini THA, Corrêa RR, Pereira PAM, Valadão CAA, Dória RGS.This study investigated the oral microbiome of horses maintained on a high soluble carbohydrate diet based on sugarcane, in comparison to those fed a pasture-based diet composed of spp., aiming to identify associations between dietary intake, the composition of oral microbial communities, and the occurrence of dental caries and diastemata. A total of 20 healthy horses, both male and female, with an average age of 9 ± 3 years and weight of 400 ± 100 kg, without a defined breed, were selected. They were divided into two groups: the High Soluble Carbohydrate Group (HSCCG), consisting of 10 hor...
The horse gut bacteriome and anaerobic mycobiome are influenced by seasonal forages and small intestinal starch digestibility.
Journal of applied microbiology    August 13, 2025   lxaf203 doi: 10.1093/jambio/lxaf203
Wunderlich G, Bull M, McGilchrist N, Zhao C, Ross T, Rose M, Chapman B.The equine gut microbiome is a complex and dynamic ecosystem, housing a diverse consortium of bacteria and anaerobic fungi (AF) capable of breaking down complex plant matter and converting it into vital energy sources for their host. The aim of this study was to broaden our current understanding of bacterial and AF diversity in the equine hindgut and how it differs between cohorts and responds to dietary shifts. Results: Faecal samples were collected from 48 horses and the bacteriome and anaerobic mycobiome analysed using long-read amplicon sequencing. Samples were collected from racehorses (R...
The Use of Sound Recorders to Remotely Measure Grass Intake Behaviour in Horses.
Animals : an open access journal from MDPI    August 4, 2025   Volume 15, Issue 15 2273 doi: 10.3390/ani15152273
Taylor DEF, Lancaster BE, Ellis AD.Visual observation to record grass intake is time-consuming and labour-intensive. Technological methods, such as activity monitors, have been used but only record head position. This study aimed to evaluate sound recorders attached to headcollars to acoustically measure grass intake behaviour in horses as a low-cost alternative method. Pilot Study 1 assessed 6 × 11 min periods comparing bites/min and chews/min between video footage (VD) and sound recorders (SR). Grazing was identified audibly (SR) and visually through soundwave pattern software (SR). Chew rates (SR: 47 ± 5 chews/min, VD: 43 ...
Sources of Environmental Exposure to the Naturally Occurring Anabolic Steroid Ecdysterone in Horses.
Animals : an open access journal from MDPI    July 17, 2025   Volume 15, Issue 14 2120 doi: 10.3390/ani15142120
Sillence MN, Holt K, Li FI, Harris PA, Coyle M, Fitzgerald DM.Ecdysterone controls moulting and reproduction in insects, crustaceans, and helminths. It is also produced by many plants, probably as an insect deterrent. The steroid is not made by vertebrates but has anabolic effects in mammals and could be useful for treating sarcopenia in aged horses. However, ecdysterone is banned in horseracing and equestrian sports, and with no limit of reporting, the risk of unintended exposure to this naturally occurring prohibited substance is a concern. To explore this risk, pasture plants and hay samples were analysed for ecdysterone content, as well as samples of...
Ohio horse industry survey: feeding and housing management practices.
Translational animal science    July 15, 2025   Volume 9 txaf072 doi: 10.1093/tas/txaf072
Share ER, Mastellar SL, Rumble JN, Eastridge ML.Equine industry housing and feeding management strategies vary widely. Management choices are important as horses spend most of their time in housing environments and demonstrating ingestive/foraging behavior. As of 2023, over 1.4 million Ohioans identified as horse owners and/or enthusiasts. The objectives of this survey were to determine demographics of the Ohio horse industry, commonly used sources of information, knowledge gaps regarding equine management practices, and to explore what may influence equine management choices. Using Qualtrics (Provo, UT), a 52-question online, anonymous sur...
Prediction of the digestibility and digestible energy content of hay for horses using an enzymatic degradability method.
Animal : an international journal of animal bioscience    July 9, 2025   Volume 19, Issue 8 101597 doi: 10.1016/j.animal.2025.101597
Andueza D, Martin-Rosset W.The nutritive value of forage for horses is closely dependent on the digestibility of organic matter (OMD), which is typically measured in vivo. Researchers have developed a variety of prediction methods to estimate the digestibility value of hay. In ruminants, pepsin-cellulase degradability, expressed as dry matter enzymatic degradability (dCS) and organic matter enzymatic degradability (dCO), is a commonly employed method for predicting OMD in forage types. The objective of this study was to evaluate the feasibility of predicting the dry matter digestibility (DMD), OMD, energy digestibility ...
Meat Production Potential of Local Horse Breeds: Sustainable Conservation Through Valorization.
Animals : an open access journal from MDPI    June 28, 2025   Volume 15, Issue 13 1911 doi: 10.3390/ani15131911
Ivanković A, Pećina M, Bittante G, Amalfitano N, Konjačić M, Kelava Ugarković N.Local horse breeds, particularly cold-blood types, are often marginalized in economic and social contexts, primarily due to the neglect of their economic, genetic, and cultural potential, as well as their role in preserving the identity of rural areas, local communities, and ecosystems. The valorization of these breeds is a crucial prerequisite for their economic repositioning. The Croatian Posavina horse is a local breed, well adapted to harsh, extensive production systems. Its sustainability is achieved through pasture-based meat production, primarily targeting foreign European markets. Ensu...
Dietary nonstructural carbohydrates influence synthesis of mare milk carbohydrates and yield.
Journal of animal science    June 26, 2025   Volume 103 skaf213 doi: 10.1093/jas/skaf213
Pyles MB, Fowler AL, Crum AD, Hayes SH, Schendel RR, Lawrence LM.Mare milk is the primary source of nutrients for neonatal foals and contains various carbohydrates. Milk oligosaccharides are complex carbohydrates that are resistant to enzymatic digestion and provide support for the immune system and developing microbiome in the neonate. However, factors influencing the synthesis of milk carbohydrates in the mare are not well understood. This study aimed to determine the effects of dietary nonstructural carbohydrate (NSC) on mare milk composition and yield. Thoroughbred mares were paired by age and last breeding date then randomly assigned to one of two diet...
An Observational Study of Wood-Chewing in Mares and Their Foals Kept in Fenced Outdoor Paddocks with Variable Browsing Access.
Journal of applied animal welfare science : JAAWS    May 7, 2025   1-16 doi: 10.1080/10888705.2025.2496496
Bouquet A, Nicol C, Díez-León M.The natural behaviour of wood-chewing (WC) in horses may, under certain conditions, become excessive or it may precede crib-biting . However, the potential causes of WC, including frustrated foraging motivation) or developmental exploratory or teething behavior are poorly understood. We studied WC in 31 mares and their foals, examining associations with browsing access, stocking density, foal sex and performance of other non-nutritive oral behaviors. Behaviors (counts/hour) were recorded by all-occurrences sampling during focal, continuous observations of grazing,resting and suckling for 20 ...
Welfare assessment of racehorses provides a baseline for continued monitoring.
Equine veterinary journal    April 21, 2025   doi: 10.1111/evj.14510
Annan R, Trigg L, Allen K, Hockenhull J, Valenchon M, Mullan S.Racehorse welfare continues to be a focus of stakeholders both within and outside of the industry, and there is a need for evidence-based animal welfare data. Objective: To gather data on racehorse welfare through a combination of animal-based observations and information about management practices, and to ascertain the feasibility of trained industry assessors carrying out a racehorse welfare assessment. Methods: Cross-sectional. Methods: Sixteen trained industry assessors conducted individual observations of 737 racehorses in 21 Flat racing (F), 27 National Hunt (NH) and 26 Dual-purpose (D) ...
Effects of different grain types on nutrient apparent digestibility, glycemic responses, and fecal VFA content in weaned foals.
BMC veterinary research    April 14, 2025   Volume 21, Issue 1 273 doi: 10.1186/s12917-025-04716-w
Huang X, Li Q, Li X, Li C, Li J, He L, Jing H, Yang F, Li X.China's equine industry has shifted from traditional rough grazing to modern intensive farming, expanding the roles of horses into eventing, leisure, tourism, and meat and dairy production. Concurrently, equine nutrition has evolved from a forage-based diet to a more diverse regimen incorporating grain supplements to meet the heightened energy demands of intensive farming. However, nutrient digestibility and glycemic response vary considerably based on grain type, starch content, composition, and structural properties. Optimal grain selection is therefore essential for energy supplementation a...
Morphine and codeine in racing horse feed: is there reason for concern?
Arhiv za higijenu rada i toksikologiju    March 31, 2025   Volume 76, Issue 1 60-66 doi: 10.2478/aiht-2025-76-3888
Karačonji IB, Jelača T, Jurič A, Vrdoljak AL.Opiates such as morphine and codeine are substances often misused to improve the performance of racing horses during competitions and are therefore on the International Federation for Equestrian Sports' list of prohibited substances. However, a positive antidoping test may be due to the consumption of feed (mainly lucerne or oats) contaminated by opium poppy containing the alkaloids morphine and codeine. In order to determine whether a positive antidoping test is the result of an intentional abuse of opiates or consumption of feed contaminated by poppies, we optimised conditions for the extrac...
Host-feeding preferences of Culex pipiens and its potential significance for flavivirus transmission in the Camargue, France.
Medical and veterinary entomology    March 21, 2025   doi: 10.1111/mve.12802
Rodríguez-Valencia V, Olive MM, Le Goff G, Faisse M, Bourel M, L'Ambert G, Vollot B, Tolsá-García MJ, Paupy C, Roiz D.The spread of the West Nile (WNV) and Usutu (USUV) flaviviruses in Europe in recent decades highlights the urgent need to understand the transmission networks of these pathogens as a basis for effective decision-making. These viruses are part of a complex disease cycle that involves birds as principal hosts and humans and horses as dead-end hosts. Our study aims to uncover the intricate relationships between the main mosquito vector of these viruses, Culex pipiens L. (Diptera: Culicidae) and its feeding preferences based on the forage ratio among several host species, primarily birds in a lan...
Trophic rewilding by large herbivores reduces plant nitrogen and water limitation across seven sites irrespective of their edaphic conditions.
The Science of the total environment    March 20, 2025   Volume 973 179141 doi: 10.1016/j.scitotenv.2025.179141
Kaštovská E, Mastný J, Choma M, Čapek P, Jirků M, Bitomský M, Konvička M.Based on a growing understanding of the role of wild megafauna in the functioning of natural ecosystems, trophic rewilding by large herbivores is increasingly used as a nature-based solution to mitigate biodiversity loss and climate change in Europe and beyond. Despite the growing interest in implementing nature-based approaches to restore key non-productive ecosystem services, there is relatively little data available to assess the benefits and risks of rewilding projects. We therefore investigated the effects of year-round grazing by large ungulates on plant biomass characteristics and their...
Tracking the Track: The Impact of Different Grazing Strategies on Managing Equine Obesity.
Animals : an open access journal from MDPI    March 19, 2025   Volume 15, Issue 6 doi: 10.3390/ani15060874
Cameron L, Challinor M, Armstrong S, Kennedy A, Hollister S, Fletcher K.Horses evolved to live in herds, continuously moving and foraging. Domestic horses often have limited social contact and movement, with high calorific intake, contributing to obesity. "Track grazing" is an increasingly popular management system, but evidence of its impact on weight management, behaviour, and welfare is limited. Horses (n = 19) resident at HorseWorld (UK) were observed July to October 2023, monitoring bodyweight (kg), Body Conditions Score (BCS), hoof health, behaviour, and welfare indicators. Horses on track grazing systems lost significantly more bodyweight (kg) (F(1,15) = 8....
Assessment of tree-associated atypical myopathy risk factors in Acer pseudoplatanus (sycamore) seeds and leaves.
Equine veterinary journal    January 25, 2025   doi: 10.1111/evj.14475
González-Medina S, Hyde C, Chang YM, Piercy RJ.Sycamore tree-derived hypoglycin A (HGA) toxin causes atypical myopathy (AM), an acute, equine pasture-associated rhabdomyolysis but incidence fluctuates. Objective: Investigate whether tree or environmental factors influence HGA concentration in sycamore material and are associated with AM relative risk. Methods: Retrospective and experimental prospective study. Methods: UK sycamore population, seed production and AM incidence data were obtained. HGA concentration was measured in seeds from trees from 10 different central UK locations. The effect of tar spot infection, seed maturity, tree tru...
Estimating Total-Tract Digestibility of Nutrients and Their Contribution to Digestible Energy Supplies in Equine Diets.
Journal of equine veterinary science    January 23, 2025   105362 doi: 10.1016/j.jevs.2025.105362
Webster AP, Price T, Ingersoll T, Suagee-Bedore JK, White RR.Our objectives were to use a quantitative literature review to explore dietary and feed factors influencing apparent total-tract digestibility of dry matter (DMD), crude protein (CPD), neutral detergent fiber (NDFD), ether extract (EED), non-structural carbohydrates (NSCD), non-fiber carbohydrates (NFCD), and residual organic matter (rOMD) in equine diets, and to assess their contributions to digestible energy (DE) supplies. Data from 54 studies were modeled using linear mixed-effect regressions, with publication as a random effect to account for study variability. For each nutrient, five mode...
Further Investigation Into an Environmental Source for Aminorex.
Drug testing and analysis    December 18, 2024   Volume 17, Issue 8 1357-1367 doi: 10.1002/dta.3820
Brooks L, Ryan M, McCosham E, Loganathan D.In 2009, levamisole was identified as a source for several aminorex positives reported in postrace equine samples in Canada. Since then, unexplained detections of aminorex continue to be identified in equine samples where there is no evidence to link these detections to a levamisole administration. Previous studies identified a compound named barbarin in some species of plants from the Brassicaceae family and suggested that barbarin may be a possible precursor for aminorex. The objectives of this study were to (1) investigate the link between barbarin and aminorex and (2) identify other biomar...
Effect of hay nets on subluxations, pain-pressure thresholds, and cervical range of motion in the axial skeleton of adult horses.
Journal of equine veterinary science    December 5, 2024   Volume 145 105256 doi: 10.1016/j.jevs.2024.105256
DeBoer M, Rieck T, Johnson L, Redenius H, Martinson K.Horses have become more intensively managed, including the use of hay nets to increase intake time and reduce hay waste, but their impact on health is not well known. The objectives were to compare changes in subluxations, range of motion, and the pain-pressure threshold in the axial skeleton of horses fed hay with (NET) or without (CON) hay nets. In September 2021, 13 adult horses were blocked by bodyweight (BW) and randomly assigned to the NET or CON treatments for one year. After one year, horses switched treatments and the trial concluded in September 2023. Horses were housed in dry lots w...
The effect of feeding order of forage and oats on metabolic and digestive responses related to gastric emptying in horses.
Journal of animal science    December 4, 2024   skae368 doi: 10.1093/jas/skae368
Jensen RB, Walslag IH, Marcussen C, Thorringer NW, Junghans P, Nyquist NF.Feeding order of forage and concentrate might affect gastric emptying and subsequently digestion in horses. The objective of this study was to measure gastric emptying in combination with metabolic and digestive responses in the plasma and caecum, respectively, when changing the feeding order of oats (O) and hay (H) (oats first then hay: O-H vs. hay first then oats: H-O). Four caecum cannulated horses were used in a 2×2 crossover design, with two 12-day periods consisting of 10 d of diet adaptation and two days of data collection. Hay was fed at 0600, 1400 and 2000 h, while oats were fed in ...
Forage, freedom of movement, and social interactions remain essential fundamentals for the welfare of high-level sport horses.
Frontiers in veterinary science    November 20, 2024   Volume 11 1504116 doi: 10.3389/fvets.2024.1504116
Phelipon R, Hennes N, Ruet A, Bret-Morel A, Górecka-Bruzda A, Lansade L.Societal concerns for animal welfare extend to all domestic species, including high-level sport horses. The welfare of these horses, notably highlighted during the recent Olympics, has garnered significant public interest, prompting inquiries into their living conditions. Animal welfare studies have emphasised three key needs crucial to equine welfare: unlimited access to forage, freedom of movement, and social interactions with peers, commonly referred to as the "3Fs"-access to Forage, Freedom of movement, and interactions with Friend conspecifics. However, the feasibility and benefits of sat...
Feed-induced hypersalivation in horses from Austria, Germany and Switzerland.
Equine veterinary journal    November 15, 2024   Volume 57, Issue 4 1035-1043 doi: 10.1111/evj.14433
Böswald LF, Gottschalk C, Kaltner F, Merk J, Schwaiger K, Kienzle E.While previous reports come mostly from the southern Americas, several outbreaks of hypersalivation in horses were observed in Middle Europe from 2016 to 2018. Objective: To describe feed-induced hypersalivation in European horses. Methods: Analysis of feedstuffs. Methods: Veterinarians and horse or stable owners were encouraged to submit feedstuffs from case outbreaks of hypersalivation in which, infectious diseases or other systemic causes of the syndrome were ruled out and intoxication was suspected. Feedstuff analysis was performed, including gross examination, microscopic analysis of fine...
Fiber composition, in vitro true digestibility, and fiber degradation of hempseed meal compared to mainstream feedstuffs using equine feces as microbial inoculum within the DaisyII Incubator.
Journal of equine veterinary science    November 14, 2024   105230 doi: 10.1016/j.jevs.2024.105230
Springer RW, Cherry NM, Muir JP, Raub RH, Wellmann KB, Jones TN.Recent changes in legislation have put hempseed meal (Cannabis sativa L.; HSM) under consideration as a potential livestock feedstuff. Digestibility of HSM has been researched in other species, however, there are little data in horses. The study objective was to determine the in vitro true digestibility (IVTD) of HSM compared to common equine feedstuffs. An in vitro split-split plot design used four geldings (8.5 ± 3.9yr; 558.4 ± 31.0kg; 6.0 ± 0.4 BCS) to investigate the IVTD of HSM compared to alfalfa hay (ALF), Coastal Bermudagrass hay (CBH), soybean meal (SBM), rice bran pellets (RBP), a...
Environmental impacts and daily voluntary movement of horses housed in pasture tracks as compared to conventional pasture housing.
Journal of equine veterinary science    November 5, 2024   Volume 143 105215 doi: 10.1016/j.jevs.2024.105215
Farmer Long J, Duberstein J, Costin K, Callaway T, Abrams A, Wassel B, Toal K, Duberstein K.Pasture tracks are a modern equine housing trend often implemented as a weight control strategy due to the belief that they promote movement, though this is not proven experimentally. To test movement of horses housed in pasture tracks as compared to conventional pasture housing, two experiments were performed using a track (0.2ha, 3.5m in width, 561 m total distance) created around the perimeter of a 2.0 ha pasture. In experiment 1, eight horses were paired and rotated between three treatment locations: track (T), small 2 ha pasture housed within the track (SP), and a larger 20 ha pasture on ...
Intake, digestibility, and behavior of horses fed açaí kernel (Euterpe oleracea Mart.) as a substitute of Mombaça grass (Megathyrsus maximus).
Tropical animal health and production    October 28, 2024   Volume 56, Issue 8 361 doi: 10.1007/s11250-024-04203-8
Silva RC, Coelho GJ, Sousa MBF, Caldas CS, Maciel RP, Mezzomo R, Gomes DÍ, Neta ERDS, Tavares FB, Alves KS, Oliveira LRS.In this study, we analyzed the effects of including açaí kernel, in the experimental diets fed to horses on the intake and digestibility of their nutrient and the resultant effects on the behavior of horses. The experiments were conducted in Federal Rural University of Amazonia. Five mares with no defined breeds, were allocated to five treatments (the replacement of Mombaça grass (Megathyrsus maximus) with crushed açaí kernel at 0, 18.75, 37.50, 56.25, and 75% levels of dry matter), in a 5 × 5 Latin square design. The animals were housed in covered stalls with concrete floors, equipp...
A wearable real-time particulate monitor demonstrates that soaking hay reduces dust exposure.
Equine veterinary journal    October 27, 2024   doi: 10.1111/evj.14425
Ivester KM, Ni JQ, Couetil LL, Peters TM, Tatum M, Willems L, Park JH.Affordable particulate matter (PM) monitors suitable for use on horses will facilitate the evaluation of PM mitigation methods and improve the management of equine asthma. Objective: Calibrate a real-time wearable PM monitor (Black Beauty [BB]) and compare the PM exposures of horses fed dry or soaked hay. Methods: Laboratory calibration; complete cross-over feed trial. Methods: Side-by-side sampling with BB monitors and tapered element oscillating microbalances (TEOMs) was performed under varying concentrations of PM from alfalfa hay. Linear regression was used to derive a calibration formula ...
Stability of Gastric Fluid and Fecal Microbial Populations in Healthy Horses under Pasture and Stable Conditions.
Animals : an open access journal from MDPI    October 16, 2024   Volume 14, Issue 20 2979 doi: 10.3390/ani14202979
Bishop RC, Kemper AM, Clark LV, Wilkins PA, McCoy AM.Equine gastrointestinal microbial communities vary across the gastrointestinal tract and in response to diet or disease. Understanding the composition and stability of gastric fluid microbiota in healthy horses is a prerequisite to understanding changes associated with the development of disease. The objective of this study was to describe microbial communities in the gastric fluid and feces of healthy horses longitudinally. Horses were maintained on pasture (6 weeks), stabled (5 weeks), then returned to pasture. A consistent forage diet was provided throughout. Native gastric fluid and feces ...
Assessment of livestock husbandry practices and production constraints among smallholder mixed crop-livestock production systems in the Majang zone, southwest Ethiopia.
Heliyon    September 12, 2024   Volume 10, Issue 19 e37400 doi: 10.1016/j.heliyon.2024.e37400
Assefa S, Duguma B, Worku Z.Livestock production plays a significant role in improving the livelihoods of poor smallholder farmer households in the Majang zone. However, despite its importance, there is limited information on husbandry practices and constraints on livestock production to design and implement effective interventions to maximize productivity. The aim of this study was to assess husbandry practices and major constraints on livestock production in the Majang zone, southwest Ethiopia. A semi-structured questionnaire was used to collect data from a randomly selected sample of 168 households (HHs) through a fac...