Analyze Diet

Topic:Nutrition

Nutrition in horses encompasses the study of dietary requirements and feeding practices that support equine health, growth, and performance. It involves the analysis of nutrients such as carbohydrates, proteins, fats, vitamins, and minerals, and their roles in equine physiology. Proper nutrition is essential for maintaining optimal body condition, supporting metabolic processes, and preventing dietary-related disorders. Research in this field examines the nutritional needs of horses at different life stages and activity levels, as well as the effects of various feed types and supplements. This page compiles peer-reviewed research studies and scholarly articles that explore the composition, digestion, and impact of different dietary components on equine health and performance.
Investigations on the Effects of Different Calcium Supply Exceeding the Requirements on Mineral Serum Concentrations and Bone Metabolism in Young Warmblood Stallions.
Animals : an open access journal from MDPI    August 19, 2021   Volume 11, Issue 8 2439 doi: 10.3390/ani11082439
Schubert DC, Neustädter LT, Coenen M, Visscher C, Kamphues J.Since mineral supplements for horses commonly contain macro minerals, although the requirement for such is usually covered by roughage-based diets, the aim of this study was to investigate the effects of different dietary calcium levels on mineral serum concentrations and bone metabolism. The trial was conducted in 30 young warmblood stallions (2-3 years) that were divided into two groups for a five-month feeding trial. The groups were fed a hay- and oat-based diet and were either supplied with high (Ca-High) or moderate (Ca-Moderate) calcium excess. While in Ca-High calcium supply was about 2...
The Effect of Strip Grazing on Physical Activity and Behavior in Ponies.
Journal of equine veterinary science    August 19, 2021   Volume 110 103745 doi: 10.1016/j.jevs.2021.103745
Cameron A, Longland A, Pfau T, Pinnegar S, Brackston I, Hockenhull J, Harris PA, Menzies-Gow NJ.This work aimed to determine the effect of strip grazing on physical activity in ponies using behavioral observations alongside accelerometers positioned at the poll. In study one, ten British native breed ponies were randomly assigned to paddock A (50 × 110 m) or B (50 × 110 m divided into seven equal strips with access to one additional strip per day) for seven days (n = 5/paddock). In study two, ten different British native breed ponies were randomly assigned for 14 days individually to (1) a control field where the animal was allowed complete access to their allotted area (n = 4)...
Microbial Variability of Commercial Equine Probiotics.
Journal of equine veterinary science    August 14, 2021   Volume 106 103728 doi: 10.1016/j.jevs.2021.103728
Berreta A, Burbick CR, Alexander T, Kogan C, Kopper JJ.Probiotics are defined as live microorganisms that confer a health benefit to the host when administered in adequate amounts, therefore the presence of viable microorganisms is essential. Previous studies reported inconsistencies when comparing contents of probiotics marketed for use in animals with label claims. However, to the author's knowledge the variability of the same product between different lots has yet to be evaluated. The objective of this observational study was to evaluate the content and inter-lot variability of commercial equine probiotic products using culture dependent and in...
Degradation of Monosaccharides, Disaccharides, and Fructans in the Stomach of Horses Adapted to a Prebiotic Dose of Fructooligosaccharides and Inulin.
Journal of equine veterinary science    August 14, 2021   Volume 105 103731 doi: 10.1016/j.jevs.2021.103731
Bachmann M, Glatter M, Bochnia M, Greef JM, Breves G, Zeyner A.For a period of 20 days, 12 horses either received a prebiotic supplementation with fructooligosaccharides and inulin via Jerusalem artichoke meal (JAM) or corncob meal without grains (CMG) as placebo. The horses were euthanized 1 hour postprandial, gastric digesta was sampled from pars nonglandularis (PNG) and pars glandularis (PG), and concentrations of starch, mono- and disaccharides, fructans, d- and l-lactic acid, and short chain fatty acids were analyzed. Concentrations of starch and simple sugars were widely the same in JAM supplemented and not supplemented meals. However, fructans were...
Uterine Involution of Mares Supplemented with Dietary Algae-Derived Omega-3 Fatty Acids During the Peripartum Period.
Journal of equine veterinary science    August 13, 2021   Volume 106 103733 doi: 10.1016/j.jevs.2021.103733
Ferreira JRM, Villela SB, Bianconi C, Ormieres M, de Melo GD, Pugliesi G, Gobesso AAO.Different approaches have been used to improve conception rates at foal heat. Omega-3 fatty acids family and derivatives have improved reproductive efficiency in ruminants, but literature lacks studies evaluating these components on equines. The objective of this study was to evaluate effects of mare dietary supplementation with microalgae rich in docosahexaenoic acid (DHA) during peripartum on follicular dynamics and uterine involution in early post-partum. Eighteen pregnant mares, no particular breed, 410 ± 39.5 kg body weight (BW), and 7.83 ± 2.01 yr old were used. Mares were randomly ass...
Total Carbon Dioxide in Adult Standardbred and Thoroughbred Horses.
Journal of equine veterinary science    August 12, 2021   Volume 106 103730 doi: 10.1016/j.jevs.2021.103730
Lindinger MI.The TCO2 (total carbon dioxide) test is performed on the blood of racehorses as a means of combatting the practice of administering alkalizing agents for the purpose of enhancing performance. The purposes of this review are to present an overview of the factors contributing to TCO2 and to review the literature regarding TCO2 in adult Standardbred and Thoroughbred horses to demonstrate the range of variability of TCO2 in horses. Most of the research published on the topic of TCO2 or bicarbonate measurement in racehorses was accessed and reviewed. PubMed and Google Scholar were the primary sear...
Fatty acid profiles of the plasma and follicular fluid mares fed a combination of linseed and salmon oil.
Anais da Academia Brasileira de Ciencias    August 9, 2021   Volume 93, Issue suppl 3 e20190443 doi: 10.1590/0001-3765202120190443
Viafara JAS, Portilho RV, Maculan R, Souza Júnior JF, Silva CM, Rodrigues PG, El Azzi MS, Souza JC.This study evaluated the presence of polyunsaturated fatty acids in circulating blood and in the ovarian follicular fluid of mares, after supplementation of the diet with linseed oil. Six Mangalarga Marchador mares, weighing 397.00±31.89 kg, were kept on native pasture, and assigned to the current study. In a switch over design, mares were randomly allocated to receive 150 ml of vegetable oil daily, containing polyunsaturated fatty acids n3 (62.23 g ALA, 20.34 g LA, 2.27 g EPA, 2.32 g DHA), (n=3) or no supplementation (n=3) in two replicates. Blood and follicular fluid samples were taken on t...
Effects of Differences in Fibre Composition and Maturity of Forage-Based Diets on the Microbial Ecosystem and Its Activity in Equine Caecum and Colon Digesta and Faeces.
Animals : an open access journal from MDPI    August 8, 2021   Volume 11, Issue 8 2337 doi: 10.3390/ani11082337
Muhonen S, Sadet-Bourgeteau S, Julliand V.Fibrous feeds are essential for horses. When developing feeding regimens promoting health and performance, we need to understand the digestion of plant cell walls and the functioning of the hindgut microbial ecosystem. Our objective was to investigate the effect of grass fibre maturity and legume forage on the hindgut microbiota and its activity. Six caecum and colon fistulated geldings were fed three diets differing in fibre composition: concentrate and late harvested grass haylage (35:65 energy ratio) (C); early and late harvested grass haylage (80:20) (G); lucerne and late harvested grass h...
Effect of a long-term high-energy diet on cardiovascular parameters in Shetland pony mares.
Journal of veterinary internal medicine    August 5, 2021   Volume 35, Issue 5 2427-2436 doi: 10.1111/jvim.16229
D' Fonseca NMM, Beukers M, Wijnberg ID, Navas de Solis C, de Ruijter-Villani M, van Doorn DA, Stout TAE, Roelfsema E.Changes in cardiovascular parameters, including blood pressure (BP) and cardiac anatomical dimensions, are an inconsistent feature of the equine metabolic syndrome. The order in which these changes arise is unknown. Objective: Determine the order in which EMS-associated changes in cardiovascular parameters arise. Methods: Twenty Shetland pony mares. Methods: High-energy (HE) diet mares were fed 200% of net energy requirements for 1 (n = 3) or 2 (n = 7) consecutive diet-years, with 17 weeks of hay-only between years. Noninvasive BP measurements and echocardiograms were performed during bo...
Seasonal Variation in the Faecal Microbiota of Mature Adult Horses Maintained on Pasture in New Zealand.
Animals : an open access journal from MDPI    August 4, 2021   Volume 11, Issue 8 2300 doi: 10.3390/ani11082300
Fernandes KA, Gee EK, Rogers CW, Kittelmann S, Biggs PJ, Bermingham EN, Bolwell CF, Thomas DG.Seasonal variation in the faecal microbiota of forage-fed horses was investigated over a 12-month period to determine whether the bacterial diversity fluctuated over time. Horses ( = 10) were maintained on pasture for one year, with hay supplemented from June to October. At monthly intervals, data were recorded on pasture availability and climate (collected continuously and averaged on monthly basis), pasture and hay samples were collected for nutrient analysis, and faecal samples were collected from all horses to investigate the diversity of faecal microbiota using next-generation sequencing ...
Investigations of the maintenance system of the Konik Polski horse and its effects on fecal microbiota activity during the winter and summer seasons.
Animal science journal = Nihon chikusan Gakkaiho    July 29, 2021   Volume 92, Issue 1 e13603 doi: 10.1111/asj.13603
Juśkiewicz J, Fotschki B, Jaworska J, Siemieniuch M.Gastrointestinal microbiota play a key role in the nutrients digestion and hence maintaining animal health and welfare. The diet offered to the animals in captivity may differ considerably from that on natural pastures. In a stabled maintenance system, horses have a limited choice of habitat and feed. Time spend for feeding is relevant for equine welfare because the reduction of the time devoted for foraging may be responsible for inducing gastric inflammation and ulceration. Therefore, in the present study, it was hypothesized that fecal bacterial fermentative processes differ between free-ro...
Safety of a feed additive consisting of a tincture derived from Verbascum thapsus L. (great mullein tincture) for use in all animal species (MANGHEBATI SAS).
EFSA journal. European Food Safety Authority    July 28, 2021   Volume 19, Issue 7 e06711 doi: 10.2903/j.efsa.2021.6711
Bampidis V, Azimonti G, Bastos ML, Christensen H, Fašmon Durjava M, Kouba M, López-Alonso M, López Puente S, Marcon F, Mayo B, Pechová A....The tincture derived from L. (great mullein tincture) is intended to be used as a sensory additive in feed for all animal species. The product is a water/ethanol solution, with a dry matter content of ˜ 2.8% and contains on average 0.216% polyphenols including 0.093% flavonoids. According to a previous assessment, the additive was not characterised in full and about 82% of the dry matter fraction remained uncharacterised (representing 2.26% of the tincture). There was also uncertainty on the potential presence of iridoid glycosides in the tincture. Therefore, the Panel on Additives and Prod...
Associative Effects between Forages and Concentrates on In Vitro Fermentation of Working Equine Diets.
Animals : an open access journal from MDPI    July 26, 2021   Volume 11, Issue 8 2212 doi: 10.3390/ani11082212
Gandarillas M, Keim JP, Gapp EM.Horses are hindgut fermenters, and it is therefore important to determine the postgastric nutritive value of their feedstuffs and diets. Moreover, it has been demonstrated in other animal species that the fermentation of diets results in different values than those expected from pure ingredients. Therefore, the general objective of this work is to evaluate the gas production (GP) and volatile fatty acid (VFA) concentration, as well as the associative effects, of mixtures of different forages and concentrated foods, which are representative of the traditional diets of high-performance horses. M...
Straw as an Alternative to Grass Forage in Horses-Effects on Post-Prandial Metabolic Profile, Energy Intake, Behaviour and Gastric Ulceration.
Animals : an open access journal from MDPI    July 24, 2021   Volume 11, Issue 8 doi: 10.3390/ani11082197
Jansson A, Harris P, Davey SL, Luthersson N, Ragnarsson S, Ringmark S.Straw's low energy content means it is a roughage option for horses with low energy requirements. Previously, in a field study, straw was associated with an increased risk for gastric ulcers. This study evaluated the effect on gastric ulcers, metabolic profile and behaviour of replacing, in a forage-only ration, 50% of the daily allowance with wheat straw. Six equines were studied in a 2 × 21-day cross-over design. The control diet (CON: 100% grass forage) and the straw diet (S: 50% grass forage and 50% straw [DM basis]) were iso-energetic. Gastroscopy was performed prior to the study and on ...
New Insights into the Management of an EHV-1 (Equine Hospital) Outbreak.
Viruses    July 22, 2021   Volume 13, Issue 8 doi: 10.3390/v13081429
Vandenberghe E, Boshuizen B, Delesalle CJG, Goehring LS, Groome KA, van Maanen K, de Bruijn CM.In May 2018, Wolvega Equine Hospital (WEH) experienced an EHV-1 outbreak. This outbreak caused significant economic losses and negative publicity for the hospital. How should hospitals prepare themselves for these outbreaks and prevent shedding of the virus on multiple neighboring premises? The hospital transformed most of its activities into mobile practice and the entire infected hospital population was moved to a separate remote location. The hospital was cleaned and disinfected according to the latest recommendations before reopening. Four neighboring professional equine businesses and thr...
Effect of Donor Diet on In Vitro Digestibility of Forages by Fecal Inoculate.
Journal of equine veterinary science    July 21, 2021   Volume 105 103722 doi: 10.1016/j.jevs.2021.103722
Godwin T, Webb G, Lancaster P.Previous research indicates equine fecal inoculates produce comparable results to cecal fluid when used for in vitro procedures to analyze dry matter digestibility (DMD). Equine hindgut microbial communities represented in fecal samples have been shown to be affected by diet. The study's objective was to determine the effect of the donor diet on in vitro DMD when fecal donors were fed high starch, or high fiber diets. Six Quarter Horses were used in a crossover design to compare the effects of a grain versus forage diet on in vitro digestion of forages ranging from: CP 7.7 to 16.4 %DM, NDF 53 ...
The effects of signalment, diet, geographic location, season, and colitis associated with antimicrobial use or Salmonella infection on the fecal microbiome of horses.
Journal of veterinary internal medicine    July 16, 2021   Volume 35, Issue 5 2437-2448 doi: 10.1111/jvim.16206
Arnold CE, Pilla R, Chaffin MK, Leatherwood JL, Wickersham TA, Callaway TR, Lawhon SD, Lidbury JA, Steiner JM, Suchodolski JS.The fecal microbiome of healthy horses may be influenced by signalment, diet, environmental factors, and disease. Objective: To assess the effects of age, breed, sex, geographic location, season, diet, and colitis caused by antibiotic use (antimicrobial-associated diarrhea [AAD]) and Salmonella infection on fecal microbiota. Methods: Healthy horses (n = 80) were sampled from nonhospital environments across multiple geographical locations in the United States. Horses with AAD (n = 14) were defined as those that developed diarrhea secondary to antimicrobial use. Horses with Salmonella infect...
Treatment and outcome of eight horses with limb cellulitis and septic tendonitis or desmitis.
Veterinary surgery : VS    July 16, 2021   Volume 50, Issue 7 1542-1552 doi: 10.1111/vsu.13691
Cooper HE, Davidson EJ, Slack J, Ortved KF.To report the clinical features, treatment, and outcome in horses with cellulitis and concurrent septic tendonitis and/or desmitis. Methods: Short case series. Methods: Medical records from 2000 to 2019 were reviewed, identifying horses with cellulitis and concurrent septic tendonitis and/or desmitis based on sonographic examination and positive bacterial culture. Signalment, ultrasonographic results, bacterial culture, treatment, duration of hospitalization, and complications were recorded. Long-term outcome data were obtained from follow-up examinations and/or telephone interviews. Successfu...
Ingestion of Soil by Grazing Sport Horses.
Animals : an open access journal from MDPI    July 15, 2021   Volume 11, Issue 7 doi: 10.3390/ani11072109
Jurjanz S, Collas C, Quish C, Younge B, Feidt C.Data on soil ingestion in horses are lacking in contrast to other free-range animals. The importance of soil as a vector for environmental pollutants to food is less relevant in horses but several disorders secondary to soil ingestion, such as sand colic or enteritis have been reported. Therefore, soil ingestion has been studied on Irish sport horses grazing at three offered levels of daily herbage: 2, 3 and 4% of their body weight. Soil ingestion was estimated by the faecal recovery of a soil natural marker. Horses had 4.5, 4.1 and 3.7% of soil in their total intake respectively for the 2, 3 ...
Refeeding syndrome in a miniature donkey.
Journal of veterinary emergency and critical care (San Antonio, Tex. : 2001)    July 14, 2021   Volume 31, Issue 5 668-673 doi: 10.1111/vec.13074
Bookbinder L, Schott HC.To describe refeeding syndrome in an equid without a history of recognized risk factors. Methods: Refeeding syndrome with marked hypophosphatemia developed in an aged miniature donkey gelding during treatment of suspected enterocolitis. Hypophosphatemia (manifested clinically as ileus and neuromuscular weakness) developed despite a short (3 day) history of hyporexia, increased body condition (7/9), and adherence to nutritional recommendations for critically ill equids. Nutritional support included nasogastric enteral feeding with a commercial equine nutrition product claiming to provide 100% o...
Pre-loading large volume oral electrolytes: tracing fluid and ion fluxes in horses during rest, exercise and recovery.
The Journal of physiology    July 13, 2021   Volume 599, Issue 16 3879-3896 doi: 10.1113/JP281648
Waller AP, Lindinger MI.Exercise results in rapid and large extracellular to intracellular fluid shifts, as well as significant sweating losses of water and ions. It is unknown whether ions within oral electrolyte supplements are taken up by muscle (and other soft tissues) and whether oral supplementation can effectively offset sweating losses. Pre-loading with 8 L of a balanced hypotonic electrolyte supplement attenuated extracellular fluid losses, increased exercise duration and increased sweating fluid and ion losses during submaximal exercise. Supplemented electrolytes appear in skeletal muscle within 1 h after...
Integrative biology defines novel biomarkers of resistance to strongylid infection in horses.
Scientific reports    July 12, 2021   Volume 11, Issue 1 14278 doi: 10.1038/s41598-021-93468-2
Sallé G, Canlet C, Cortet J, Koch C, Malsa J, Reigner F, Riou M, Perrot N, Blanchard A, Mach N.The widespread failure of anthelmintic drugs against nematodes of veterinary interest requires novel control strategies. Selective treatment of the most susceptible individuals could reduce drug selection pressure but requires appropriate biomarkers of the intrinsic susceptibility potential. To date, this has been missing in livestock species. Here, we selected Welsh ponies with divergent intrinsic susceptibility (measured by their egg excretion levels) to cyathostomin infection and found that their divergence was sustained across a 10-year time window. Using this unique set of individuals, we...
Postprandial insulin responses to various feedstuffs differ in insulin dysregulated horses compared with non-insulin dysregulated controls.
Equine veterinary journal    July 8, 2021   Volume 54, Issue 3 574-583 doi: 10.1111/evj.13474
Macon EL, Harris P, Bailey S, Barker VD, Adams A.Controlling postprandial hyperinsulinaemia is important in insulin dysregulated (ID) horses to reduce the risk of laminitis. Objective: To evaluate postprandial insulin responses of ID versus non-insulin dysregulated (NID) horses to feedstuffs varying in nonstructural carbohydrate (NSC) and crude protein (CP). Methods: Randomised crossover. Methods: Eighteen adult mixed-breed horses (13.3 ± 2.2 years; 621 ± 78.8 kg) were individually fed [~1 g/kg body weight (BW)] specific feedstuffs within two crossover studies. Eight ID and eight NID were used in Study A, and 11 ID and 5 NID in Stud...
Dietary omega-3 fatty acid supplementation does not impair vitamin E status or promote lipid peroxidation in growing horses.
Journal of animal science    July 7, 2021   Volume 99, Issue 7 skab177 doi: 10.1093/jas/skab177
White-Springer SH, Vineyard KR, Kivipelto J, Warren LK.Omega-3 (n-3; ω-3) fatty acids (FA) are often included in the diet for their potential health benefits. However, because oxidative potential is increased with the degree of unsaturation in vitro, polyunsaturated FA such as eicosapentaenoic acid (EPA; 20:5n-3) and docosahexaenoic acid (DHA; 22:6n-3) may be at increased risk of lipid peroxidation. We aimed to determine the effects of dietary n-3 FA supplementation on antioxidant status and lipid peroxidation in yearling horses. Quarter Horses (mean ± SEM; 14.6 ± 0.2 mo) were randomly assigned to receive no n-3 FA supplementation (CON; n = 6) ...
Effect of maternal overnutrition on predisposition to insulin resistance in the foal: Foal skeletal muscle development and insulin signaling.
Domestic animal endocrinology    July 2, 2021   Volume 77 106648 doi: 10.1016/j.domaniend.2021.106648
Bradbery AN, Coverdale JA, Hammer CJ, Dunlap KA, Leatherwood JL, Satterfield MC.Skeletal muscle plays an integral role in the ability of a horse to perform at high levels. Shifts in skeletal muscle development in response to maternal plane of nutrition may have substantial and lasting impacts on athletic performance and whole-body metabolism. Therefore, sixteen Quarter Horse mares were used in a completely randomized design and maintained at a body condition score (BCS) 6 until start of third trimester. On d 235 of gestation, mares were randomly assigned to receive one of two dietary treatments with a diet formulated to meet requirements during late gestation (CON; n = ...
A Standard Scale to Measure Equine Keeper Status and the Effect of Metabolic Tendency on Gut Microbiome Structure.
Animals : an open access journal from MDPI    July 1, 2021   Volume 11, Issue 7 1975 doi: 10.3390/ani11071975
Johnson ACB, Biddle AS.Thriftiness in horses has been associated with more efficient nutrient harvesting in digestion, absorption and/or utilization, but the relative contribution of the gut microbiome to host metabolic tendency is not well understood. Recognizing the unreliability of owner reported assignment of keeper status, this research describes a novel tool for calculating whether a horse is an easy (EK) or hard (HK) keeper and then characterizes microbiome differences in these groups. The Equine Keeper Status Scale (EKSS) was developed and validated based on data gathered from 240 horses. Estimates of dietar...
Dietary supplementation of a Saccharomyces cerevisiae fermentation product attenuates exercise-induced stress markers in young horses.
Journal of animal science    June 29, 2021   Volume 99, Issue 8 skab199 doi: 10.1093/jas/skab199
Valigura HC, Leatherwood JL, Martinez RE, Norton SA, White-Springer SH.Mitigation of exercise-induced stress is of key interest in determining ways to optimize performance horse health. To test the hypothesis that dietary supplementation of a Saccharomyces cerevisiae fermentation product would decrease markers of exercise-induced stress and inflammation in young horses, Quarter Horse yearlings (mean ± SD; 9 ± 1 mo) were randomly assigned to receive either no supplementation (CON; n = 8) or 21 g/d S. cerevisiae fermentation product (10.5 g/feeding twice daily; SCFP; n = 10) top-dressed on a basal diet of custom-formulated grain as well as ad libitum Coastal berm...
Diagnosis of animal abuse: A Brazilian study.
Preventive veterinary medicine    June 29, 2021   Volume 194 105421 doi: 10.1016/j.prevetmed.2021.105421
Gomes LB, Paiva MT, Lisboa LO, Oliveira CSF, Garcia RCM, Soares DFM.Due to their intimate relationship with human beings, animals can experience abuse, especially in the family environment. Research on the variables involved in this topic is scarce in Latin America. The objective of this study was to identify the main types of animal abuse in Brazilian municipalities and to characterize animals and perpetrators in addition to identifying the socioeconomic factors associated with the incidents. The occurrences of animal abuse were analyzed from the records of the Police Station Specialized in Fauna Crimes Investigation Department of the Civil Police of Minas Ge...
Rickets in a Thoroughbred-cross foal: case report and review of the literature. Asin J, Murphy BG, Samol MA, Polanco J, Moore JD, Uzal FA.Rickets is a metabolic bone disease associated with failure of endochondral ossification and impaired osteoid mineralization in growing animals. As a consequence, affected individuals can develop gross and microscopic bone malformations. The most common causes of rickets in domestic species include vitamin D and phosphorus deficiency. Rickets has been described in multiple species; however, comprehensive postmortem characterizations with confirmatory histopathology in equids have not been published. A 6-mo-old, Thoroughbred-cross foal was diagnosed with rickets based on gross autopsy findings ...
Hay versus haylage: Forage type influences the equine urinary metabonome and faecal microbiota.
Equine veterinary journal    June 23, 2021   Volume 54, Issue 3 614-625 doi: 10.1111/evj.13456
Leng J, McNally S, Walton G, Swann J, Proudman C, Argo C, Emery S, La Ragione R, Eustace R.Gut microbial communities are increasingly being linked to diseases in animals and humans. Obesity and its associated diseases are a concern for horse owners and veterinarians, and there is a growing interest in the link among diet, the intestinal microbiota and metabolic disease. Objective: Assess the influence of long-term hay or haylage feeding on the microbiota and metabolomes of 20 Welsh mountain ponies. Methods: Longitudinal study. Methods: Urine, faeces and blood were collected from 20 ponies on a monthly basis over a 13-month period. Urine and faeces were analysed using proton magnetic...
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