Analyze Diet

Topic:Observational Study

Observational studies in horses involve systematically assessing and recording behaviors, conditions, or other characteristics without manipulating the study environment. These studies provide insights into natural equine behaviors, health conditions, and interactions with their environment. Researchers often focus on aspects such as herd dynamics, feeding patterns, or responses to environmental changes. The data collected can help identify trends, correlations, and potential risk factors for certain conditions. This page compiles peer-reviewed research studies and scholarly articles that explore various observational methodologies and their applications in understanding equine behavior and health.
RAiSE (recognizing affective state in equine) and the assessment of equine affective state: Accuracy and application.
Journal of equine veterinary science    March 11, 2026   Volume 160 105847 doi: 10.1016/j.jevs.2026.105847
Hiney K, Anderson K, Brady C.Accurate interpretation of affective state (AS) of animals is critical to assessment of their wellbeing. Although horse owners may appreciate the importance of this skill, many lack the ability to interpret AS accurately. Objective: To test if the online course Recognizing Affective States in Equine (RAiSE) improves participants' assessment of AS in horses and increases use of AS in management. Methods: Participants completed pre (PRE), post (POST), and 90 day post (90POST) course surveys to assess accuracy in identifying AS in 20 videos, as well as use of AS in their horse interactions. Pre/p...
Does the Reproductive Technique Affect Neonatal Health Parameters in Foals?
Reproduction in domestic animals = Zuchthygiene    March 10, 2026   Volume 61, Issue 3 e70192 doi: 10.1111/rda.70192
Alonso MA, Dos Santos GR, Fonte JS, Marques PC, Pereira DD, Toral FLB, Bordignon V, Junior JB, Squires E, Fernandes CB.This retrospective study evaluated whether different reproductive biotechnologies influence neonatal behaviour parameters in foals. Data from 102 foalings in two commercial breeding farms were analysed, including foals conceived by artificial insemination (AI), conventional embryo transfer (ET) and intracytoplasmic sperm injection (ICSI). Neonatal parameters recorded were birth weight, time to achieve sternal recumbency, time to stand, time to nurse and time to meconium elimination. Mixed statistical models were applied to assess the influence of reproductive technique, breed, sex and farm. Si...
Reference intervals for serum macro- and microminerals in clinically healthy horses in Northwestern Spain: Influence of age, sex, breed and diet.
Journal of equine veterinary science    March 6, 2026   Volume 160 105846 doi: 10.1016/j.jevs.2026.105846
Fernández-Villa C, Miranda M, Rigueira L, Martinez L, Villanueva B, Freire S, López-Alonso M.Serum mineral concentrations are key indicators of nutritional status, metabolic function and health in horses; however, contemporary reference intervals (RI) derived from large, well-characterised populations remain scarce. Objective: To establish serum RI for macro- and microminerals in clinically healthy horses in Northwestern Spain and to evaluate the influence of sex, age, breed and diet on mineral status. Methods: Blood samples were collected from clinically healthy horses (n = 211), and serum concentrations of essential macrominerals (calcium [Ca]; phosphorous [P]; magnesium [Mg]; sodiu...
Geriatric Horses in Germany: Approaches to Nutrition, Housing and Overall Care.
Animals : an open access journal from MDPI    March 5, 2026   Volume 16, Issue 5 813 doi: 10.3390/ani16050813
Czerner AC, Lindner A, Zeyner A, Wensch-Dorendorf M, Gehlen H.Changes in horse management and use mean that a growing number of horses are now kept into older age, while age-specific management practices for these animals remain insufficiently documented. This cross-sectional online survey aimed to describe the housing, feeding, activity, behaviour and care routines of horses aged 20 years and older in Germany, based on 923 questionnaires completed by horse owners about their senior horses. Most horses maintained regular tactile social contact (87.4%, = 805), and over half were still ridden or driven (61.6%, = 566). However, the proportion of horses in...
Application of a framework to mitigate the risk of surgical site infection after exploratory celiotomy in horses: A retrospective study.
Veterinary surgery : VS    March 3, 2026   doi: 10.1111/vsu.70061
Lopez Cruz C, Gonzalez LM, McKinney-Aguirre CA, Hepworth-Warren KL, Roessner HA, Burke M, Fogle CA.To describe the methodology used to identify the contributors to a perceived sudden increase in exploratory celiotomy surgical site infections (SSI) and complications at the North Carolina State University Veterinary Teaching Hospital (NCSU VTH) between 2019 and 2020 and evaluate the effect of the designed intervention up to 4 years after its implementation. Methods: Case-control retrospective study over a five-year period. Methods: A total of 448 horses that underwent exploratory celiotomy for the treatment of acute abdominal pain were included. Methods: Medical records of horses that under...
Assessment of baseline adrenocorticotropic hormone and insulin concentrations in healthy horses in Saskatchewan over a 1-year period.
Journal of veterinary internal medicine    February 26, 2026   Volume 40, Issue 1 aalaf056 doi: 10.1093/jvimsj/aalaf056
Viviani P, Ragno VM, Parker S, Montgomery JB.Endocrine disorders in horses, such as pituitary pars intermedia dysfunction and equine metabolic syndrome, rely on hormone testing for diagnosis, but seasonal and regional variabilities highlight the need for locally generated data. Objective: To characterize monthly blood concentrations of ACTH and insulin over 1 year in healthy horses in Saskatchewan. A secondary objective was to assess the association between these outcomes and the following factors: sex, age, breed, pregnancy status, level of exercise, body condition score, and cresty neck score. Methods: Sixty-eight clinically healthy, c...
Vectorcardiography at rest and during exercise in horses using the Delta (Δ) 12-lead configuration.
Journal of veterinary internal medicine    February 26, 2026   Volume 40, Issue 1 aalaf008 doi: 10.1093/jvimsj/aalaf008
Paulussen E, Delhaas T, Decloedt A, van Loon G, Van Steenkiste G.Twelve-lead electrocardiography (ECG) is essential in human cardiology but remains poorly investigated in equine medicine due to a lack of standardization and inconsistent findings. Objective: Describe the normal vectorcardiogram (VCG) variation in horses at rest and during exercise using Delta 12-lead ECG, including repeatability and intra- and interobserver variations. Methods: One hundred two healthy Warmblood horses, aged 3-20 years, were examined at rest, of which 30 also during exercise. Methods: Prospective, observational study. Electrocardiograms were recorded using a Δ 12-lead confi...
Paternal determinants of Offspring Sex Ratio in horses: A retrospective analysis of stallion breed, age, and breeding method.
Animal reproduction science    February 24, 2026   Volume 289 108150 doi: 10.1016/j.anireprosci.2026.108150
Armangau M, Lurier T, Josson A.Offspring Sex Ratio (OSR) at birth in mammals is generally assumed to follow a 1:1 male-to-female distribution, yet deviations in OSR have been observed and may be influenced by maternal and paternal factors. In this study we have focused on the effect of paternal factors (stallion age and breed) and breeding methods on the OSR. Using a retrospective dataset of 251,783 foal births recorded in France between 2015 and 2024, we applied generalized linear mixed models to evaluate the effects of these variables on the likelihood of producing a female foal. Stallion identity was included as a random...
Friends, forage, freedom: A cluster analysis investigating horse management styles and welfare in the UK and Ireland.
Animal welfare (South Mimms, England)    February 24, 2026   Volume 35 e15 doi: 10.1017/awf.2026.10073
Watson W, MacKay JRD, Dwyer C.This study aims to improve the welfare and management of recreational horses by identifying how different management styles affect horse health and behaviour. We examined the management styles of recreational horse owners in the UK and Ireland, focusing on social interaction (friends), access to suitable forage (forage), and unrestricted movement (freedom). We collected 1,501 survey responses, distributed via social media, and summarised the characteristics and management choices of the respondents. Using the Divisive ANAlysis cluster package in R, three distinct management styles were identif...
The importance of prebiotics in managing colic in horses: focus on Akkermansia muciniphila and its anti-inflammatory potential.
Frontiers in veterinary science    February 24, 2026   Volume 13 1759381 doi: 10.3389/fvets.2026.1759381
Cottone A, Seiter K, Thomas B, Schank N, Wulf M, Miller L, Anderson S, Munkhsaikhan U, Verma A, Abidi AH, Kassan M.Colic remains one of the most frequent and costly causes of equine morbidity and mortality, with significant welfare and economic implications. Disturbances in the gut microbiome are increasingly recognized as an important contributing factor. In recent years, prebiotics, non-digestible substrates that promote beneficial microbes, have emerged as promising microbiome-targeted strategies. () has gained attention for its unique ability to degrade mucin, maintain epithelial integrity, and exert potent anti-inflammatory effects. Although its benefits are well established in humans and rodent mode...
Hoof and movement asymmetry in Thoroughbred racehorses with predominantly single direction training.
American journal of veterinary research    February 23, 2026   1-7 doi: 10.2460/ajvr.25.12.0437
Chan ZYS, Ho W, Slobodova A, Parkes RSV, Forbes B, Pfau T.To investigate associations between contralateral (left-right) hoof width asymmetry and vertical movement asymmetry in racing Thoroughbreds. We hypothesized that forehoof differences would be associated with poll and withers movement asymmetry and hind hoof differences with pelvis movement asymmetry. Unassigned: This observational cross-sectional study included 169 Thoroughbred racehorses recently retired from clockwise racing and assessed the day of scheduled farriery work. Contralateral hoof width differences were quantified and movement asymmetry during trot measured using inertial sensors ...
Heart Rate as an Indicator of Stress in Gotland’s Russ Horses.
Zoo biology    February 20, 2026   doi: 10.1002/zoo.70052
Dundjerovic I, Sneddon LU.Animal welfare concerns both the physical and mental wellbeing of animals so the assessment of animals kept in captivity, for example zoos, is important and necessary both legally and ethically. Good welfare is especially vital when breeding endangered species as stress impairs reproductive investment. As zoos often have high numbers of visitors, it is important to know if humans and the associated noise might influence the animals' welfare. The aim of this study was to investigate whether the presence of visitors and activities offered to the visitors caused a stress response in horses housed...
More conservative use of antimicrobials does not adversely affect treatment outcomes in foal bronchopneumonia.
Veterinary journal (London, England : 1997)    February 19, 2026   Volume 316 106605 doi: 10.1016/j.tvjl.2026.106605
Boeger L, Volkmann N, Probst J, Kemper N, Venner M.The emergence of antimicrobial resistance raises concerns about using antibiotics in veterinary medicine, particularly in cases of bronchopneumonia in foals diagnosed early based on a screening programme. Many subclinical foals recover spontaneously, thus, interest in more restrictive antibiotic usage is increasing. Objective: To determine whether initiating antimicrobial therapy at a later stage of bronchopneumonia decreases antimicrobial usage without compromising successful resolution of pathology in affected foals. Methods: A total of 1200 warmblood foals born in 2020, 2022, 2023 and 2024 ...
A Survey of Trainers on the Health and Welfare of Standardbred Racehorses in Prince Edward Island.
Journal of applied animal welfare science : JAAWS    February 17, 2026   1-7 doi: 10.1080/10888705.2026.2628477
Burns JJ, MacMillan KM.The Standardbred racing industry plays an important role in the North American economy and is expected to continue operating and growing. However, public perception is often unfavorable, with increasing concern about the health and welfare of Standardbred racehorses. Despite this, limited research has examined Standardbred well-being. To address this gap, a survey of equine trainers on Prince Edward Island (PEI) was conducted to evaluate key health and welfare issues during racing careers. Results showed that over one quarter of respondents had at least one horse displaying stereotypic behavio...
Equine dental destructive disorders: an epidemiological survey in northern Germany.
Frontiers in veterinary science    February 17, 2026   Volume 13 1706621 doi: 10.3389/fvets.2026.1706621
Häussling MPP, Steinberg T, Büttner K, Hannig C, Hannig M, Lemke L, Zierau O, Staszyk C.Destructive lesions of the peripheral and infundibular cementum are increasingly recognized in equine dentistry. While similarities to human caries have been noted, current evidence does not conclusively support this classification. This study aims to assess the prevalence, distribution, and potential risk factors associated with these lesions in a defined horse population. Unassigned: A total of 114 horses from northern Germany underwent standardized oral examination between 2021 and 2023. Clinical data on peripheral and infundibular lesions, diastemata, and influencing factors such as age an...
Verifying thoroughbred racehorse fatalities through Equibase chart review.
Journal of equine veterinary science    February 16, 2026   Volume 159 105808 doi: 10.1016/j.jevs.2026.105808
Markowitz JS.Equibase (EQB) race charts provide the official record of North American Thoroughbred races and often document catastrophic injury, euthanasia, or equine-ambulance transport. Because chart documentation and regulatory classification differ, fatalities occurring > 72 hours after a race fall outside international regulatory definitions applied by the Equine Injury Database (EID) and may not appear in EID aggregates. EQB charts confirm horse identity, race participation, finishing status, and last recorded start. Horseracing Wrongs (HRW), an advocacy group, publishes horse-level fatality data tha...
Radiographic findings of candidate stallions presented for licensing at all German Warmblood horse-breeding associations in 2018-2020.
Journal of equine veterinary science    February 16, 2026   Volume 159 105809 doi: 10.1016/j.jevs.2026.105809
Folgmann MS, Stock KF, Feige K, Delling U.Current studies on the health status of young German Warmblood stallions are lacking. Objective: To evaluate the prevalence of radiographic findings at licensing examinations of Warmblood candidate stallions and quantify the environmental influences on the distribution of recorded findings. Methods: In this retrospective observational study, records of 1693 radiographic examinations performed on 1678 German Warmblood stallions presented for licensing in 2018-2020 were reviewed. Data were provided by all German Warmblood horse-breeding associations and their official veterinarians. The collecti...
Stress Responses in Dressage Horses: Insights from FEI Noseband Measurements Across National Competition Levels.
Animals : an open access journal from MDPI    February 6, 2026   Volume 16, Issue 3 518 doi: 10.3390/ani16030518
Fialová S, Kuřitková D, Sobotková E.This pilot study investigated stress-related behaviors in 238 dressage horse-rider combinations competing at national levels from Elementary (A) to Grand Prix (GP). Noseband tightness was assessed on-site using the FEI noseband measuring device (FNMD), and all but two horses complied with FEI regulations. Video-based analysis quantified conflict behaviors including mouth opening, tail swishing, and changes in head-neck position. Because noseband tightness showed minimal variation, no meaningful association with behavioral expression could be determined. In contrast, competition level had a cle...
Radiographic Prevalence of Anatomical Variations of the Ventral Lamina of the Sixth Cervical Vertebra, C6/C7 Articular Process Joint Modelling and Competition Outcomes in Warmblood Sport Horses.
Animals : an open access journal from MDPI    January 29, 2026   Volume 16, Issue 3 424 doi: 10.3390/ani16030424
Strootmann T, Peter VG, Körner J.Anatomical variations of the caudal ventral lamina of the sixth cervical vertebra (C6), referred to as equine caudal cervical morphologic variations (ECCMV), and radiographic modelling of the caudal cervical articular process joints (APJ) are commonly identified in sport horses, yet their interrelationship and relevance for athletic performance remain controversial. This retrospective observational study aimed to determine the prevalence of ECCMV and APJ modelling in a clinically sound warmblood sport horse population, to evaluate a potential association and to assess if ECCMV affects competit...
Commentary on MacKechnie-Guire et al. Measuring Noseband Tightness on the Lateral Aspect of the Horse’s Face. Animals 2015, 15, 537.
Animals : an open access journal from MDPI    January 28, 2026   Volume 16, Issue 3 412 doi: 10.3390/ani16030412
Henshall C, McGreevy P, Shea G, Doherty O, Christensen JW, Fenner K, Warren-Smith A, McLean A.The use of horses for sport is under scrutiny due to evidence that common practices such as tight nosebands may impair horse welfare. Restrictive nosebands prevent horses from performing normal comfort behaviour such as coughing and yawning. To address these concerns, the International Society for Equitation Science (ISES) developed a noseband tightness-checking device, the ISES "taper gauge," along with a validated method that assesses how far the device can be inserted beneath the noseband at the dorsal midline of the nasal planum. However, citing concerns about the reliability of dorsal mid...
Barrel racing horses demonstrate a high incidence of nonclinical postrace airway disorders.
Journal of the American Veterinary Medical Association    January 23, 2026   1-10 doi: 10.2460/javma.25.10.0712
Williams MR, Silverstone A, Burba DJ, McCarrel T, Schoonover MJ, Rudra P.To describe the prevalence of postrace airway abnormalities in a population of barrel racing horses competing in Oklahoma. Unassigned: A descriptive field study was conducted at a single event. Horses underwent resting endoscopy within 60 minutes of competition. Recordings were evaluated and numerical and/or binary scores assigned for pharyngeal lymphoid hyperplasia, recurrent laryngeal neuropathy, dorsal displacement of the soft palate, epiglottic entrapment, and exercise-induced pulmonary hemorrhage. Clients completed a questionnaire detailing the horse's medical history and run time. A line...
Feed intake, digestibility and passage kinetics in grazing horses.
Scientific reports    January 22, 2026   Volume 16, Issue 1 3052 doi: 10.1038/s41598-026-35647-7
Bachmann M, Bochnia M, Wensch-Dorendorf M, Glatter M, Schäfer S, Simroth K, Greef JM, Zeyner A.Feed intake and digestibility are important indicators for sufficient nutrient supply, but they can be assessed only to a limited extent in horses on pasture. In horses, feed intake is embedded in a complex behavioural pattern of searching, selecting, chewing and almost constant movement called foraging. The objectives of this study were to estimate organic matter intake (OMI) and organic matter digestibility (OMD) in six horses, 24 h/day on pasture, based on plant alkanes and synthetic n-hexatriacontane (C) excretion. A multi-compartmental model was fitted to the marker excretion and the C m...
Scoping review of comminuted fractures of the proximal phalanx in horses.
Equine veterinary journal    August 29, 2025   doi: 10.1111/evj.70077
de Chaisemartin C, Vandeweerd JM, Schramme M.Comminuted fractures of the proximal phalanx (CFPP) occur in adult horses. Various treatments have been described. It remains uncertain which is the best therapeutic option. Objective: This study aims to review systematically the existing literature on CFPP in horses, evaluate treatment modalities, synthesise outcomes to assess treatment efficacy and survival rates, and identify knowledge gaps for future research. Methods: Scoping review. Methods: A literature search was conducted using online databases. The bias quality of each article was evaluated. Chi-squared analysis was used to detect di...
Born to run? Associations between gestational and early-life exposures and later-life performance outcomes in Thoroughbreds.
Equine veterinary journal    August 25, 2025   doi: 10.1111/evj.70084
Mouncey R, de Mestre AM, Arango-Sabogal JC, Verheyen KL.Gestational and early-life exposures may modulate development during growth and influence future athletic performance. Objective: To investigate associations between gestational and early-life exposures in Thoroughbreds and (i) likelihood of racing, (ii) total number of runs and (iii) total prizemoney by the end of the 3-year-old year. Methods: Prospective cohort. Methods: Daily records were kept on the location and duration of turnout, management and veterinary-attended episodes of disease or injury from birth until leaving the farm or study exit for 129 Thoroughbred foals on six stud farms. ...
Exploratory Study of the Urine Protein-to-Creatinine Ratio in Apparently Healthy Horses.
Veterinary sciences    August 21, 2025   Volume 12, Issue 8 783 doi: 10.3390/vetsci12080783
Kovarikova S, Blahova J, Steffenova V, Vaskova N, Jahn P.Currently, there is relatively little published information on the urine protein-to-creatinine (UPC) ratio in horses, a parameter commonly used in dogs and cats. The aim of this study was to determine the reference range of UPC in urine samples of apparently healthy horses and to assess the influence of age and sex. Urinalysis and the determination of urinary protein and creatinine concentrations and ratios were performed. A total of 118 urine samples from apparently healthy horses were included in the study: 10 from foals younger than 6 months and 108 from horses older than 1 year. The median...
Comparison of Gait Characteristics for Horses Without Shoes, with Steel Shoes, and with Aluminum Shoes.
Animals : an open access journal from MDPI    August 13, 2025   Volume 15, Issue 16 2376 doi: 10.3390/ani15162376
Gottleib K, Trager-Burns L, Santonastaso A, Bogers S, Werre S, Burns T, Byron C.Differences in horseshoe materials may have effects on gait that could change perceived esthetic qualities. Objective information regarding effects of shoeing on gait characteristics of horses is scant. The aim of this study was to determine differences in gait characteristics for horses under various experimental shoeing conditions (barefoot, aluminum shoes, steel shoes) on two surfaces (asphalt and soft footing) using body- and hoof-mounted sensors. We hypothesized that shoeing would affect hoof arc height during early (arc height a) and late (arc height b) swing phases but would not affect ...
Variation in Four Horse Gait Categories Illustrated by Quantitative Analysis With ANALOC-E.
Veterinary medicine international    August 6, 2025   Volume 2025 4906015 doi: 10.1155/vmi/4906015
Staiger EA, Pereira de Toledo A, Rizzato Paschoal V, Patterson Rosa L.Horse gaits are a trait highly selected and prized in diverse breeds. Meanwhile, gait classification relies mostly on subjective visual observations by evaluators. Noninvasive equipment able to track locomotion in horses and output quantitative gait parameters is not only helpful in evaluating locomotion but also in designating gait types and its variations. Equine locomotion pattern designation based solely on observer evaluation can be subjective; therefore, utilizing tools that provide quantitative results and track individual limb movements, especially during intermediate-speed gaits, can ...
The Use of Sound Recorders to Remotely Measure Grass Intake Behaviour in Horses.
Animals : an open access journal from MDPI    August 4, 2025   Volume 15, Issue 15 2273 doi: 10.3390/ani15152273
Taylor DEF, Lancaster BE, Ellis AD.Visual observation to record grass intake is time-consuming and labour-intensive. Technological methods, such as activity monitors, have been used but only record head position. This study aimed to evaluate sound recorders attached to headcollars to acoustically measure grass intake behaviour in horses as a low-cost alternative method. Pilot Study 1 assessed 6 × 11 min periods comparing bites/min and chews/min between video footage (VD) and sound recorders (SR). Grazing was identified audibly (SR) and visually through soundwave pattern software (SR). Chew rates (SR: 47 ± 5 chews/min, VD: 43 ...
Seasonal elevation in equine adrenocorticotropic hormone occurs throughout the contiguous United States and is influenced by latitude and age.
Journal of the American Veterinary Medical Association    August 1, 2025   1-10 doi: 10.2460/javma.25.04.0223
Pinn-Woodcock TL, Llanos-Soto SG, Ivanek R, Goodrich E, Frye E, Wells A, Mullen K, Arbittier E, Hughes L, Berryhill E, Brosnahan P, Buslinger L....To determine whether seasonal variation in equine plasma ACTH concentrations is affected by latitude between 29.7°N and 45.0°N in the US. Unassigned: A cohort study was performed between June 2019 and May 2020 in which plasma ACTH was measured monthly by chemiluminescence immunoassay. The study involved 201 apparently healthy horses from 9 locations within the US classified into northern, central, and southern latitude cohorts. Data analysis was performed with 3 major objectives: (1) identify outlier ACTH concentrations and horses; (2) estimate ACTH upper reference limits to define expected ...
Clinical evaluation of the ADVIA Centaur XPT chemiluminescent immunoassay for equine insulin measurement.
Journal of equine veterinary science    July 31, 2025   Volume 152 105661 doi: 10.1016/j.jevs.2025.105661
Rey-Conejo R, Toribio RE, Möller S, Müller E, Fores-Jackson P.Insulin dysregulation is a key component of equine metabolic syndrome and is commonly assessed using basal insulin concentrations. Different analyzers may yield variable insulin results, limiting comparability. Objective: To assess the analytical performance of the ADVIA Centaur XPT chemiluminescent immunoassay (CLIA) for equine insulin measurement and to establish adapted decision thresholds based on comparison with a previously used CLIA method. Methods: Precision, linearity, and dilution recovery were assessed for the ADVIA Centaur XPT. A total of 89 equine serum samples submitted for basal...
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