The effect of urea on the biological activity of gonadotrophins of placental, endometrial and urinary origin. Human chorionic gonadotrophin (HCG), pregnant mare serum gonadotrophin (PMSG) and human menopausal gonadotrophin (HMG) were incubated with varying concentrations of urea at different temperatures for different times.
The luteinizing hormone (LH) activity of HCG was progressively destroyed with increasing concentrations of urea. The degree of inactivation was greater at higher temperatures but the time of incubation did not affect the results.
The follicle-stimulating activity of PMSG was reduced at high urea concentrations; the time of incubation was without effect.
Under the experime...
Effects of exogenous progestogens on reproductive phenomena in mares. Progesterone in oil solution injected into mares intramuscularly in midcycle blocked estrus and ovulation at levels of 100 mg. per day or higher. Fifty milligrams per day prevented estrus but not ovulation. The interval from end of treatment to estrus appeared to depend upon dosage, and the effects on subsequent cyclic patterns were confounded by season of the year. Neither 50 nor 100 mg. per day stopped estrus or blocked ovulation when treatment was started on day 1 of estrus. Injection of 100 mg. per day of progesterone in oil delayed postpartum estrus and ovulation, but 50 mg. per day did n...
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