A Simplified Approach to Splenectomy in the Horse. THE NEED FOR splenectomized horses in piroplasmosis research prompted this study. Quinlan et at.1 described in detail problems encountered using the paralumbar approach to splenectomy. Gaining easy access to the splenic ligaments and vessels was difficult; for this reason the type and age of horse which could be used were limited. The purpose of this report is to present technic by which horses of varying size, age, and conformation can be splenectomized with minimum of stress on the patient and on the surgeon.
Equine Antihapten Antibody. I. 7S Beta-2A- and 10S Gamma-1- Globulin Components of Purified Anti-Beta-Lactoside Antibody. Anti-p-azophenyl-beta-lactoside (Lac) antibody has been isolated from equine antiserum by specific precipitation with Lac-HSA (human serum albumin) and Lac-Hy (hemocyanin). Antibody was separated from antigen by chromatography on DEAE cellulose and zone electrophoresis in solvents containing lactose. Six antigenically distinct immunoglobulins have been identified in purified equine anti-Lac antibody: 7S beta(2A)-globulin, 19S gamma-globulin, a 10S gamma(1) globulin, and three antigenically distinct 7S gamma-globulins. The specific hapten-binding activity of the 7S beta(2A)-antibody and of the ...
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