Population Genetic Analysis of the Estonian Native Horse Suggests Diverse and Distinct Genetics, Ancient Origin and Contribution from Unique Patrilines. The Estonian Native Horse (ENH) is a medium-size pony found mainly in the western islands of Estonia and is well-adapted to the harsh northern climate and poor pastures. The ancestry of the ENH is debated, including alleged claims about direct descendance from the extinct Tarpan. Here we conducted a detailed analysis of the genetic makeup and relationships of the ENH based on the genotypes of 15 autosomal short tandem repeats (STRs), 18 Y chromosomal single nucleotide polymorphisms (SNPs), mitochondrial D-loop sequence and lateral gait allele in DMRT3. The study encompassed 2890 horses of 61 b...
Retrospective investigation of prognostic indicators for adult horses with infection of a synovial structure. To investigate predictors of survival and athletic function in adult horses with infection of a synovial structure. Objective: Increasing duration from contamination to referral, bone or tendon involvement and positive microbial culture decreases short-term survival. Synovitis and/or sepsis at 5 days post-admission and involvement of Staphylococcus spp. decreases long-term athletic function. Methods: Retrospective study. Methods: Records over 4 years of adult horses with synovial sepsis were reviewed. A two-tailed Fisher's exact test, Mann-Whitney U test or t-test was used to examine whether v...
[Development of PCR methods for detection of EAV infection]. The goal of this work was the development of suitable (real-time) RT-PCR techniques for fast and sensitive diagnosis of EAV and for molecular-epidemiological characterisation of viral strains, as an alternative to virus isolation. To this purpose two conventional RT-PCR methods and one real-time RT-PCR were adapted to detect the broadest possible spectrum of viral strains. Several dilutions with Bucyrus strain showed a 100-fold higher sensitivity of real-time RT-PCR and heminested RT-PCR compared to simple RT-PCR. Making use of 11 cell culture supernatants of different EAV isolates and 7 semen...
Hoof kick injuries in unmounted equestrians. Improving accident analysis and prevention by introducing an accident and emergency based relational database. To assess injury patterns attributable to horse kicks, to raise the issue of preventive measures, and to evaluate the role of modern accident and emergency department computer software. Methods: Data analysis using a new kind of full electronic medical record. Results: Seventeen kicked equestrians were unmounted at the time of injury. Eight of seventeen patients sustained contusions of the extremities, the back, and the trunk. In nine patients an isolated facial injury was diagnosed. Five of nine patients needed referrals to the department of plastic surgery because of the complexity of the fa...
L-carnitine added to post-thawed semen acts as an antioxidant and a stimulator of equine sperm metabolism. The objective of this study was to enhance the in vitro sperm quality and in vivo fertility of frozen-thawed equine semen by the addition of l-carnitine (LC) to post-thawed semen. Different concentrations of LC were added to thawed samples to obtain four treatments control and 0.5, 1 and 2 mM LC. In the in vitro experiments, sperm motility and kinematics, membrane integrity and intracellular calcium ion concentration ([Ca ] ) were investigated, and the antioxidant bioactivity of LC was assessed by measuring hydrogen peroxide and nitrite concentrations (NO ). The fertility rate was assessed v...
Toxic shock syndrome in a horse with Staphylococcus aureus pneumonia. A 3-year-old Thoroughbred gelding was examined because of clinical signs of pneumonia and shock. Mucous membrane petechiation and ventral edema were observed and considered to be a result of vasculitis. Epidermal necrosis developed on the distal portions of the limbs. The horse had a persistent high fever that was unresponsive to nonsteroidal anti-inflammatory treatment, and Staphylococcus aureus was isolated from a nasal swab specimen and 2 transtracheal wash fluid samples. Antimicrobial, anti-inflammatory, and supportive treatment resulted in clinical improvement. However, resolution of the ...
Uterine torsion in mares. Uterine torsion typically occurs during mid to late gestation and is estimated to cause 5% to 10% of all equine obstetric emergencies. Clinical signs include abdominal pain that may be mistaken for gastrointestinal distress, parturition, or abortion. Uterine torsion is an emergency for the mare and fetus, and early recognition and intervention are essential to optimizing the chance of survival. This article reviews the etiology, diagnosis, treatment, and prognosis of uterine torsion in mares.
Congenital scoliosis in a quarter horse filly. A 4-week old Quarter Horse filly was evaluated for abnormal gait and lateral deviation of the cervical spine. Physical examination findings prompted radiographs and computed tomography of the thoracic vertebral column which revealed hypoplasia of several thoracic vertebral bodies and resultant scoliosis of the thoracic vertebral column and deviation of the left and right hemithoraces and associated ribs. Collectively, radiography and computed tomography provided an accurate description of the vertebral malformations resulting in scoliosis in this foal.
Comparison of the Mineral Profile of Two Types of Horse Diet, Silage and Commercial Concentrate, and Their Impacts on Hoof Tensile Strength. The type of diet that a horse consumes is one of the most important factors determining the mineral profile and tensile strength of its hooves, so the quality and nutritional value of the supplied feed are fundamental to achieving satisfactory hoof quality. The objective of this study was to compare the differences in the mineral concentrations of sodium (Na), potassium (K), magnesium (Mg), zinc (Zn), and Iron (Fe) between two diets, namely silage and a commercial concentrate, using atomic absorption spectrometry and to determine which led to higher increases in the hoof tensile strength of ho...
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