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Topic:Hoof Health

Hoof health in horses involves the study of the structure, function, and maintenance of the horse's hoof, which is vital for overall mobility and wellbeing. The hoof is composed of various structures, including the outer hoof wall, sole, frog, and internal components such as the laminae. These structures work together to support the horse's weight, absorb shock, and provide traction. Factors affecting hoof health include nutrition, environment, genetics, and management practices. Common hoof-related conditions include laminitis, thrush, and hoof cracks, which can impact a horse's performance and quality of life. This page compiles peer-reviewed research studies and scholarly articles that explore the anatomy, physiology, and management of hoof health in horses, as well as strategies for prevention and treatment of hoof disorders.
The effect of botulinum toxin injection into the deep digital flexor muscle on foot biomechanics in healthy horses.
American journal of veterinary research    February 26, 2026   1-10 doi: 10.2460/ajvr.25.12.0452
Slavik K, Underwood C, Lowndes C, Skelton G, van Eps A.To examine the effects of botulinum toxin inoculation into the deep digital flexor (DDF) muscle on foot biomechanics. Unassigned: 6 healthy horses were injected with botulinum toxin in the DDF of 1 forelimb. The opposite forelimb was untreated. Ground reaction forces (GRF) were measured using a pressure sensor in regions of interest, including the dorsal hoof wall (toe). The location of the center of pressure (COP) was measured relative to the dorsal hoof wall during static standing and at peak stance during walking. Repeat measurements 3, 7, 14, 28, and 42 days after injection were compared t...
Equine lameness detection and monitoring during diagnostic anaesthesia with an instrumented hoof boot.
Veterinary journal (London, England : 1997)    February 25, 2026   Volume 317 106614 doi: 10.1016/j.tvjl.2026.106614
Keller J, Hassenstein MJ, Jung K, Geburek F.Ground reaction forces are accepted as gold standard for objective assessment of weightbearing lameness but measurements at the hoof are limited to experimental devices. This study aimed to evaluate whether innovative instrumented hoof boots (IHBs) could detect and monitor lameness in horses during diagnostic anaesthesia. Twenty-six horses referred for lameness examination (15 front limbs, 17 hindlimbs) were equipped with IHBs and body mounted inertial sensors. Data were collected simultaneously before and after diagnostic anaesthesia until objectively considered positive. Recorded IHB data i...
Hoof and movement asymmetry in Thoroughbred racehorses with predominantly single direction training.
American journal of veterinary research    February 23, 2026   1-7 doi: 10.2460/ajvr.25.12.0437
Chan ZYS, Ho W, Slobodova A, Parkes RSV, Forbes B, Pfau T.To investigate associations between contralateral (left-right) hoof width asymmetry and vertical movement asymmetry in racing Thoroughbreds. We hypothesized that forehoof differences would be associated with poll and withers movement asymmetry and hind hoof differences with pelvis movement asymmetry. Unassigned: This observational cross-sectional study included 169 Thoroughbred racehorses recently retired from clockwise racing and assessed the day of scheduled farriery work. Contralateral hoof width differences were quantified and movement asymmetry during trot measured using inertial sensors ...
Effect of palmar digital perineural analgesia (as a model for “nerving”) on forelimb loading over varied surfaces at the walk and trot – a pilot study.
Journal of equine veterinary science    February 17, 2026   Volume 159 105812 doi: 10.1016/j.jevs.2026.105812
Zimmerman-Cameron SR, Colbath AC, Caruso MA, Hoffman RM, Logan AA.Navicular syndrome is characterized by palmar foot pain and is a common cause of lameness in horses. Palmar digital neurectomy (nerving) removes sensation to the heel region of the hoof and may serve as a treatment for navicular-afflicted horses, while analgesia (blocking) results in temporary loss of sensation. Objective: The purpose of this pilot study was to examine the effect of loss of sensation in the heel on the forelimb area and force while tracking on soft and hard ground at the walk and trot. Methods: Three horses that scored a 0 or 1 on the AAEP lameness scale were fitted to Tekscan...
Evaluation of computed tomographic anatomy of the foot in Jeju horses.
Journal of veterinary science    February 10, 2026   Volume 27, Issue 1 e7 doi: 10.4142/jvs.25250
Kim T, Lee S, Seo JP.Foot diseases are common causes of lameness in horses. Computed tomography (CT) is a valuable tool for diagnosing musculoskeletal lesions in horses and providing a detailed evaluation of foot diseases. Objective: The objective of this study was to establish reference ranges related to the feet of normal Jeju horses by measuring the angles and length of the hoof, and the Hounsfield Unit (HU) value of soft tissues in the foot. Methods: Sixteen normal Jeju horses (mean age: 4.25 years; mean body weight: 282.06 kg) without lameness were examined using CT. After CT scanning, the angles and lengths ...
How to perform a venogram of the equine foot.
Journal of the American Veterinary Medical Association    January 30, 2026   1 doi: 10.2460/javma.25.11.0755
Santonastaso A, Burns T, Claffey E.The objective of this video is to demonstrate a reliable technique for obtaining diagnostic venogram images of the equine foot. Venogram studies are indicated to assess vascular contrast filling of the equine digit, primarily in cases of laminitis. Unassigned: A healthy university-owned horse was used for demonstration purposes. The protocol was approved by the Virginia Polytechnic Institute and State University IACUC. Unassigned: The horse's shoes are removed, and the foot is cleaned of debris. The horse is sedated and a medial and lateral abaxial nerve block performed. The pastern is clipped...
Comparison of the Level of Mineral Components in the Hoof Wall of Pure-Breed Arabian Horses and Polish Sport Horses.
Biological trace element research    December 24, 2025   doi: 10.1007/s12011-025-04952-z
Stanek M, Różański SŁ, Sztuka H, Komosa M.Research on the characteristics of the mineral profile of hooves can help prevent overgrowth or micro-cracks. Knowledge about the interaction of these elements shows how imbalances can lead to hoof pathology or suboptimal hoof quality. Sulphur plays a key role in keratin synthesis, contributing to hoof elasticity and strength. Iron is involved in oxygen transport to the hoof matrix cells, supporting proper metabolism and tissue regeneration. Zinc promotes keratinocyte differentiation and accelerates healing of micro-injuries within the horn structure. Copper is essential for the formation of d...
Measuring Equine Hooves in Radiographs and Computed Tomography Images Reveals Unexpected Size Differences.
Anatomia, histologia, embryologia    December 15, 2025   Volume 55, Issue 1 e70073 doi: 10.1111/ahe.70073
Sellke L, Ludewig E, Handschuh S, Witter K.In a previous study on hoof biometry, we found that mathematical correction of measuring results from radiographs did not lead to complete correspondence to computed tomography (CT) results. The present study investigates this finding by comparing 13 measures of six cadaveric equine digits collected with the following workflows: radiographs with 1 and 2 m focus-object distance (FOD) (Xray 1 m/2 m), computed tomography images in planes defined based on anatomical landmarks (CTw), simulated radiographs based on the tomography dataset (virtual 120-mm slabs, Xray Sim) and measurements based ...
Cyclooxygenase-2 and von Willebrand factor-an immunohistochemical study of the equine foot with and without laminitis, post-mortem perfused with paraffin oil.
Frontiers in veterinary science    December 15, 2025   Volume 12 1673415 doi: 10.3389/fvets.2025.1673415
Underberg BA, Van der Vekens E, Drews B, Kaessmeyer S.Equine laminitis is a complex and potentially fatal disease characterized by severe vascular and inflammatory alterations within the equine foot. This study aimed to develop immunohistochemistry (IHC) protocols for the detection of cyclooxygenase-2 (COX-2) and von Willebrand factor (vWF) in equine feet with and without laminitis, post-mortem perfused with paraffin oil. Unassigned: A total of 12 equine forelimbs from 8 horses were utilized in this study, divided into two study cohorts: one with laminitis and the other as a non-laminitis control. To develop the IHC protocols thoroughly, the tiss...
Preliminary Biometric Study on Symmetry of Hoof Solear Aspect in Forelimbs in Four Horse Breeds.
Animals : an open access journal from MDPI    November 21, 2025   Volume 15, Issue 23 3369 doi: 10.3390/ani15233369
Stachurska A, Wnuk E, Łuszczyński J, Donderowicz W.Correct conformation and symmetry of bilateral hooves are associated with a properly balanced hoof, which has a significant impact on the biomechanics of movement and reduces the risk of injury. The aim of the study was to compare solear aspect dimensions of bilateral hooves in forelimbs (hoof width, length, frog width, length, medial and lateral diagonals) with regard to sex, breed and age of horses. The dimensions were measured with a calliper in 100 horses of four breeds. The results showed that the breed factor significantly affected all dimensions. Age was significant for some variables, ...
Radiographic measurements of Miniature Horse and Miniature Pony Feet.
Journal of equine veterinary science    October 31, 2025   Volume 155 105710 doi: 10.1016/j.jevs.2025.105710
Olijnyk JM, Hosgood G, Richardson J, Beggs C, McConnell E.Reliable assessment of foot conformation is essential for podiatric management in Miniature Horses and Ponies, yet reference ranges and the reliability of radiographic measurements remain poorly defined. Objective: This study quantified radiographic measurements of foot conformation in sound, skeletally mature Miniature Horses and Ponies and evaluated their agreement, reliability, and precision. Methods: Standardised lateromedial and dorsopalmar/plantarodorsal radiographs were obtained from 30 Miniature Horses and Ponies. Two observers took 60 measurements from 10 sets of projections on three ...
Risk factors for strangulating lipoma obstruction and lipomata in horses.
Equine veterinary journal    October 4, 2025   doi: 10.1111/evj.70107
Gillen A, Hassel D, Gonzalez SW, Savage V, Mudge M, Wood A, Barnes H, Bauck A, Freeman D, Dembek K, Gonzalez LM, Archer DC.Strangulating lipoma obstruction (SLO) is the most common cause of equine small intestinal strangulation and is fatal without surgery. Currently, epidemiological information is primarily limited to signalment-related risk factors and requires further investigation. Objective: To identify horse-level risk factors for SLO and/or abdominal lipoma(s) (LP) formation in horses with acute colic that underwent surgery or post-mortem examination at participating equine clinics. Methods: Prospective, international, multicentre, epidemiological study. Methods: An epidemiological study was conducted over ...
Comparing the Difference in Traction Between the Bare Hoof, Iron Horseshoes and Two Glue-On Models on Different Surfaces.
Sensors (Basel, Switzerland)    September 26, 2025   Volume 25, Issue 19 5975 doi: 10.3390/s25195975
Siedler C, Zinkanel YM, Schramel JP, Peham C.The interaction between equine hooves and various ground surfaces is a critical factor for injury prevention and performance in modern equestrian sports. Accurate measurement of surface grip is essential for evaluating the effectiveness of different hoof protection systems. This study introduces the Vienna Grip Tester (VGT), a novel sensor-based device developed to quantify rotational resistance-an important parameter for assessing hoof-surface interaction. The VGT utilizes a torque wrench and spring-loaded mechanism to simulate lateral hoof movements under a standardized vertical load (~700 N...
Better hoof, better horse – genetic correlations between ability to race barefoot and performance in trotting horses.
Animal : an international journal of animal bioscience    September 23, 2025   Volume 19, Issue 11 101664 doi: 10.1016/j.animal.2025.101664
Berglund P, Andonov S, Strandberg E, Eriksson S.Harness racing is popular in several parts of the world, and the ability to race barefoot is an important trait for success because of its effect on racing time. Barefoot racing is also discussed in terms of animal welfare because not all horses have hooves that tolerate racing without shoes. In Swedish Standardbred trotters (SB) and Swedish-Norwegian Coldblooded trotters (CB), the proportion of barefoot races and the probability to race barefoot in any given race (barefoot status) have low-to-moderate heritability (h = 0.07-0.28). However, the genetic correlation between the ability to race...
Morphometric parameters, metabolic profile and musculoskeletal alterations in Criollo horses competing in the morphology trial.
Journal of equine veterinary science    September 13, 2025   Volume 154 105703 doi: 10.1016/j.jevs.2025.105703
Mousquer MA, Paz CFR, Rafael LA, Wendt CG, Curcio BR, Souza RP, Santos IPOD, da Silva RB, Müller V, Nogueira CEW.Obesity is common in some horse breeds, raising concerns about health and performance. Criollo horses in morphological events often undergo intensive management promoting early adiposity. Objective: This study aimed (1) to assess morphometric traits, metabolic profile, hoof morphology, and tarsal joint alterations in Criollo horses participating in morphological trials; (2) to evaluate sex-related differences; and (3) the associations between morphometric measurements, hoof morphology, and tarsal osteoarthritis grades. Methods: Fifty-five horses (30 mares, 25 stallions) were evaluated for body...
Effects of sand, asphalt, and a three degrees hind toe or heel elevation on equine cervical and thoracolumbosacral kinematics at walk and trot.
Journal of equine veterinary science    September 4, 2025   Volume 154 105697 doi: 10.1016/j.jevs.2025.105697
Caure S, Miljak K, Dendaw P, Thesée L, Villedey E, Malinvaud A, Cousty M, Prié V, Horan K, Weller R.Effects of ground surface and hoof angles on equine cervical and thoracolumbosacral kinematics are poorly understood. However, the equine cervical and thoracolumbosacral areas present frequent lesions and he management of factors that might improve treatment and rehabilitation outcomes, such as ground surface and hoof angles, requires more investigation. Objective: Our objectives were to determine the influence of ground surface (asphalt versus sand) and a 3 degrees hind toe or heel elevation on cervical and thoracolumbosacral kinematics during walking and trotting. Methods: Six saddle horses ...
Correction: Association between radiographic equine distal phalanx characteristics and absence, presence and type of horseshoes.
Frontiers in veterinary science    August 18, 2025   Volume 12 1675356 doi: 10.3389/fvets.2025.1675356
Ennsmann LH, Licka TF.[This corrects the article DOI: 10.3389/fvets.2025.1598038.].
RNA-seq analysis identifies key genes enhancing hoof strength to withstand barefoot racing in Standardbred trotters.
BMC genomics    August 18, 2025   Volume 26, Issue 1 751 doi: 10.1186/s12864-025-11814-4
Schwochow D, Alameddine A, Spörndly-Nees E, Montigny M, Naboulsi R, Jansson A, Niazi A, Lindgren G.Racing without protective shoes is common in the Swedish harness racing industry, as it can enhance horses' performance on the track. Trainers typically decide whether a horse will race barefoot based on practical experience rather than objective measures. However, this practice can sometimes lead to excessive hoof wear, posing potential welfare concerns for racing horses. Gene expression differences may help reveal the underlying genetic mechanisms associated with different phenotypic traits. To explore an objective measure for assessing which horses are best suited for barefoot racing, we co...
Distribution of endocannabinoid system receptors in the equine hoof: dysregulation as a potential therapeutic target for laminitis.
Histochemistry and cell biology    July 1, 2025   Volume 163, Issue 1 71 doi: 10.1007/s00418-025-02397-y
Zamith Cunha R, Gobbo F, Morini M, Zannoni A, Mainardi C, D'arpe L, Gramenzi A, Chiocchetti R.A growing body of evidence indicates that the endocannabinoid system (ECS) is essential for controlling the homeostasis of the skin and that the ECS is modified in the presence of skin disease. It is plausible to expect that the lamellar junction of the hoof expresses cannabinoid receptors and that their expression could be affected by lamellar disease. The goal of this study was to characterise the cannabinoid receptor type 1 (CB1R) and type 2 (CB2R) and the G protein-coupled receptor 55 (GPR55) within the dermo-epidermal junction of the hooves of healthy and laminitic horses. The expression ...
Measurements of equine foot parameters show limited agreement between radiographs and low-field magnetic resonance imaging.
Equine veterinary journal    June 26, 2025   doi: 10.1111/evj.14536
Bowkett-Pritchard C, Bolt DM, Chang YM, Berner D.Equine foot radiographs are commonly obtained to measure anatomical conformation parameters. Comparison of measurements between radiographs and low-field magnetic resonance imaging (MRI) has not been extensively explored. Objective: To compare foot parameter measurements between radiographs and low-field MRI, and assess the effect of hoof wall markers on visualising the hoof capsule (during MRI) and facilitating measurements. Methods: Comparative cadaveric analytical study. Methods: Radiography and MRI of nine equine cadaver front feet were performed with and without hoof wall markers, which w...
Selection signatures and inbreeding: exploring genetic diversity in five native horse breeds.
BMC veterinary research    May 16, 2025   Volume 21, Issue 1 346 doi: 10.1186/s12917-025-04794-w
Asti V, Summer A, Ablondi M, Sartori C, Giontella A, Pilastro V, Mecocci S, Cappelli K, Mancin E, Oian A, Mantovani R, Capomaccio S, Sabbioni A.Horses have undergone extensive natural and artificial selection, shaping the diversity of breeds observed today. Native Italian breeds present unique traits influenced by natural selection, such as adaptation to harsh climates, or hoof strength, but face challenges due to population declines and the reduction of their original breeding purpose. This study focuses on five local Italian breeds: Bardigiano, Haflinger, Maremmano, Murgese, and Italian Heavy Draught Horse, to understand how selection has shaped their populations. A total of 1620 individuals were genotyped with a medium-density SNP ...
Effect of intra-arterial cisplatin on dorsal hoof wall tissue of isolated perfused equine limbs.
Journal of equine veterinary science    May 6, 2025   Volume 150 105598 doi: 10.1016/j.jevs.2025.105598
Reinthaler B, Gabner S, Apprich V, Jehle M, Licka T.Equine hoof canker is a chronic proliferative condition of the hoof tissues that is often time-consuming and frustrating to treat. After surgical debridement, topical application of cisplatin (cis-Diamindichlorplatin II) has been reported, requiring repeatedly handling this hazardous substance during bandage changes. Alternatively, intraarterial application of cisplatin could be used, similar to the treatment of some human neoplastic diseases. As the side effects of such a treatment are currently unknown, evaluation of associated risks is necessary before treating live horses. Thus, forelimbs ...
Computed tomographic measurements in 110 front hooves of non-lame Thoroughbred racehorses and Warmblood showjumpers.
Equine veterinary journal    April 15, 2025   Volume 57, Issue 5 1328-1340 doi: 10.1111/evj.14509
Szabó L, Pollard D, Nagy A.There are no published reference ranges on computed tomographic (CT) hoof wall and sole measurements. Objective: To establish reference ranges for CT measurements of the hoof wall and sole in non-lame horses, and to investigate associations between measurements and signalment, laterality, and medial or lateral sides. Methods: Retrospective, cross-sectional study. Methods: Soft tissue reconstructions of fan-beam CT studies of 110 front feet of 56 non-lame horses (30 Thoroughbred racehorses, 26 Warmblood showjumpers), performed in non- to semi-weightbearing positions, were analysed. Hoof wall th...
Working equines: Assessment of welfare and management practices in and around Debre Markos District, Northwest Ethiopia.
Equine veterinary journal    March 25, 2025   doi: 10.1111/evj.14497
Bihon A, Amognehegn D, Derbew G, Yirsa T.Working equines play a crucial role in human livelihoods globally, yet their management is often inadequate. Objective: To assess the welfare and management practices of working equines. Methods: A cross-sectional study was conducted from December 2022 to April 2023 in and around Debre Markos, Northwest Ethiopia. Methods: Physical examination and structured interviews were used as data collection tools. A total of 384 equines (186 donkeys, 144 horses, and 54 mules) and their owners were selected through a purposive sampling method. Results: The results showed that 60.4% of the equines were use...
Tracking the Track: The Impact of Different Grazing Strategies on Managing Equine Obesity.
Animals : an open access journal from MDPI    March 19, 2025   Volume 15, Issue 6 doi: 10.3390/ani15060874
Cameron L, Challinor M, Armstrong S, Kennedy A, Hollister S, Fletcher K.Horses evolved to live in herds, continuously moving and foraging. Domestic horses often have limited social contact and movement, with high calorific intake, contributing to obesity. "Track grazing" is an increasingly popular management system, but evidence of its impact on weight management, behaviour, and welfare is limited. Horses (n = 19) resident at HorseWorld (UK) were observed July to October 2023, monitoring bodyweight (kg), Body Conditions Score (BCS), hoof health, behaviour, and welfare indicators. Horses on track grazing systems lost significantly more bodyweight (kg) (F(1,15) = 8....
Shoe configuration effects on equine forelimb gait kinetics at a walk.
PeerJ    February 26, 2025   Volume 13 e18940 doi: 10.7717/peerj.18940
Aoun R, Ogunmola Z, Musso A, Taguchi T, Takawira C, Lopez MJ.The shift in vertical forces on the equine hoof surface by heart-bar, egg-bar, and wooden clog shoes can significantly impact gait kinetics. Hypotheses tested in this study were that vertical, braking, and propulsion peak force (PF) and impulse (IMP) are different while shod with heart-bar, egg-bar, open-heel, and wooden clog shoes, or while unshod, and the resultant ground reaction force vector (GRF YZ ) has the longest duration of cranial angulation with open-heel shoes followed by unshod, then egg-bar and heart-bar shoes, and the shortest with wooden clog shoes. Forelimb GRFs were recorded ...
Lamellar cell death and proliferation are associated with restricted ambulation and preferential weight bearing in a model relevant to supporting-limb laminitis.
American journal of veterinary research    February 19, 2025   Volume 86, Issue 4 doi: 10.2460/ajvr.24.09.0268
Engiles JB, Stefanovski D, van Eps A.To examine the effects of prolonged preferential weight bearing (PWB) and reduced ambulation (RA) on hoof lamellae using a nonpainful in vivo experimental model. Unassigned: 12 healthy Standardbred horses were housed in stocks continuously for 92 hours. A platform shoe was placed on 1 forelimb in the PWB group (n = 6) to increase the load on the supporting limb (SL) by approximately 10% bodyweight, whereas the RA group (n = 6) had normal weight bearing. Archived healthy horse (n = 8) samples were used as controls. Histomorphometry and histochemistry (terminal deoxynucleotidyl transferase dUTP ...
Distal limb immersion in ice and water is the most effective means of cooling the equine hoof lamellae under clinically relevant conditions.
American journal of veterinary research    January 21, 2025   Volume 86, Issue 3 doi: 10.2460/ajvr.24.10.0291
Ciamillo S, Stefanovski D, Kulp J, van Eps A.To determine the relationship between hoof surface (HS), hoof wall (HW), and lamellar tissue (LAM) temperature during distal limb cooling and evaluate 4 cooling methods in ambulatory horses over extended periods using HW to estimate LAM temperature. Methods: 8 healthy, university-owned horses were enrolled. Temperature was measured over 4 hours at LAM, HS, and HW for 4 cooling methods: ice sleeve (SLEEVE), ice pack system (ICEPACK), low-volume ice water immersion (LV-IMMERSION), and high-volume ice water immersion (HV-IMMERSION). The relationship between HW, HS, and LAM was mathematically mode...
Changes in pressure distribution of the solar surface after a single trimming event are associated with external hoof measurements in the equine fore foot.
Equine veterinary journal    January 9, 2025   doi: 10.1111/evj.14463
Seery S, Gardiner J, Bates KT, Pinchbeck G, Clegg P, Ireland JL, Milner PI.Trimming is critical for a functioning equine hoof. Pressure distribution provides information on loading; however, information on the effects of trimming on pressure distribution is lacking. Objective: To describe the pressure changes of equine fore feet following trimming. Methods: Cross-sectional cohort study. Methods: Fifty sound horses were recruited. Eighteen external hoof measures of the dorsal, lateral, medial and solar aspects were obtained before and after trimming from 94 fore feet. Horses were walked over a pressure mat before and after trimming and pressure maps of the solar surfa...
Radiographic Morphometric Measurements of the Donkey’s Distal Hind Limb.
Animals : an open access journal from MDPI    December 25, 2024   Volume 15, Issue 1 doi: 10.3390/ani15010022
Schaus K, Wacker J, Jandowsky A, Büttner K, Röcken M, Bartmann CP.Morphometric measurements of the toe serve as a reference for the objective assessment of the presence of a pathological condition and as a guide for the correct trimming of the hooves. Therefore, the anatomical differences between horses and donkeys oblige an autonomous database with reference values for the donkey. The aim of this study was therefore to provide readings of the distal hind limb on radiographs of donkeys. Radiographs of the distal hind limb in two projections of 41 generally sound donkeys without lameness in walk were taken after previous hoof trimming and preparation accordin...
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