Lactate is a byproduct of anaerobic metabolism, produced when glucose is broken down for energy in the absence of sufficient oxygen. In horses, lactate levels can increase during intense physical exertion, such as racing or other high-performance activities. Elevated lactate concentrations are often associated with muscle fatigue and can provide insights into a horse's exercise physiology and conditioning status. Monitoring lactate levels can aid in assessing the horse's fitness, recovery, and adaptation to training. This page compiles peer-reviewed research studies and scholarly articles that explore the production, regulation, and implications of lactate in equine exercise physiology and performance.
Granello ME, Young JM, Cleff DB, Banks EBM, Trumble TN.To describe characteristics of postoperative peritoneal fluid following exploratory laparotomy for naturally-occurring gastrointestinal lesions in horses. Methods: Prospective, observational cohort study. Methods: A total of 26 client-owned horses that underwent exploratory laparotomy for naturally-occurring gastrointestinal lesions. Methods: Abdominocentesis was performed pre- or intraoperatively, and at three time points postoperatively (24, 72, and 168 h). Peritoneal lactate, total protein (TP), total nucleated cell count (TNCC), cytology, and systemic lactate were performed at each time ...
In horses, heat exposure modulates the hypothalamic-pituitary-adrenal axis, autonomic nervous system, and hypothalamic-pituitary-thyroid axis to maintain body temperature and prevent excessive heat accumulation. However, during strenuous exercise under hot and humid conditions, heat production may exceed dissipation, leading to heat stress, anhidrosis, heat stroke, or brain damage. Unassigned: Incremental field standardized exercise tests (fSETs) provide a reliable approach to assess training and fitness levels. Six Arabian horses from Italia Endurance Stable and Academy were monitored during ...
Yano R, Moriyama T, Arai H, Scheftgen AJ, Suen G, Nishida T, Handa M, Fukuma N.Colic, a major gastrointestinal disease in horses, has a high recurrence rate and can lead to surgery or fatal outcomes, highlighting the need for effective prevention measures. Disruption of the microbiome is a multifaceted problem and can occur from a variety of factors, such as high-concentrate diets, which can then potentially cause colic. However, individual variation in the incidence of colic can occur when under identical management practices. The aim of this study was to characterize the fecal bacterial composition of Japanese draft horses with and without a history of colic in the pas...
Burns JJ, Stull J, John E, Doyle A.For horses presenting for colic to a referral hospital in Atlantic Canada, we aimed to describe clinical findings, diagnosis, treatment, and outcome for draft breeds; identify associations between clinical findings, diagnosis, treatment, and outcome for draft compared to nondraft breeds; and identify the overall impact of draft breed on mortality. Unassigned: A retrospective review of equine colic cases presenting to the Atlantic Veterinary College (2004 to 2023). Logistic regression models were used to assess associations between parameters and draft breed and mortality. Unassigned: A total o...
Alves-Junior CDB, Ferreira TC, Nunes-Pinheiro DCS.This study evaluated the dynamics of blood immune-inflammatory biomarkers in response to exercise-induced changes in jumping horses. Methods: For this purpose, adult Brazilian Sport Horses (n = 9), aged 10.1 years, both sexes, underwent a specific training test consisting of warm-up, flatwork, and jumping exercises, and recovery on a 900 m sand track over 40 min. Clinical examinations and blood collections were performed at three time points: before (T0), immediately after (T1), and 1 h after (T2) the final exercise. Hematologic and biochemical parameters, such as total protein, gl...
Faircloth J, Fielding L, Epstein K, Crabtree N, Mochal C, Magdesian G, Major D.To describe the clinical presentation of horses with synchronous diaphragmatic flutter (SDF) and identify clinical and laboratory variables associated with survival. Methods: Retrospective study from January 2004 to September 2022. Methods: Five large animal referral hospitals in the United States. Methods: Thirty-seven horses of various breeds and ages presenting with clinical signs of SDF, excluding animals participating in an endurance competition. All horses were >1 year of age. Methods: None. Results: Variables recorded and analyzed included signalment, clinical and laboratory data, trea...
Sohn Y, An SJ, Forbes E, Yoon J, Kim BS, Kim JG, Ryu SH.To model lactate recovery kinetics in relation to sex, age, running speed, and racing experience, and to estimate blood lactate clearance time under passive recovery conditions that reflect current management practices in Jeju horses, a less well-characterized pony-sized indigenous breed. Unassigned: From October through December 2022, BLC was measured at baseline, 10 minutes after 800-m barrier trials (time [T]-1), and 50 minutes (T2) after 800-m barrier trials in 40 race-trained Jeju horses (2 to 4 years old). Lactate clearance was modeled using nonlinear regression with an exponential decay...
Neudeck S, Sauter PK, Kutter APN, Steblaj B, Söbbeler FJ, Reiners J, Freise F, Gutiérrez Bautitsta AJ, Kästner SBR.Endotoxaemia is a significant cause of morbidity and mortality in equids due to perfusion impairment and possible destruction of the glycocalyx. To evaluate our hypothesis that endotoxaemia induces changes in global cardiovascular and haematologic parameters and compromises glycocalyx integrity, evidenced by an early rise in plasma shedding products. In vivo experiments METHODS: In a prospective, randomised, controlled experimental trial, endotoxaemia was induced with E. coli B55:O5 LPS 30 ng kg over 30 min IV in six healthy adult horses ventilated with oxygen supplemented with isoflurane. St...
Söderroos D, Stefánsdóttir GJ, Ragnarsson S, Gunnarsson V, Jansson A.Weight carrying capacity is an important trait in riding horses and it may be associated with conformation. This study examined the physiological response to a ridden incremental weight carrying test in 16 adult Icelandic horses used for tour riding. Horses carried 20% (BWR20%), 25% (BWR25%), 30% (BWR30%) and 35% (BWR35%) of their body weight (BW) in tölt (~ 5.7 m/seconds, 640 m/step), and associations with body measurements and back conformation (score) were examined. Horses were divided into two groups (narrow or broad back) and body measurements were collected. Plasma lactate was analy...
Simões J, Santos AM, Santos C, Silva AS, Vintém C, Fonseca J, Coelho C.Show jumping is a popular and complex equestrian modality practiced by a athletes of different levels. However, most studies focus on elite athletes. Objective: The aim of this work was to assess the effect of a show jumping test (SJT), designed for novice athletes, on the physiological biomarkers and energy indexes of a group of horses, bred and trained in Portugal. Methods: In this prospective cross-sectional study, thirteen horses and their riders performed a SJT with obstacles set at 80 cm height using a heart rate monitor (M430 with H10 sensor). Physical examination and blood samples, for...
Liedtke AM, Meijer H, Horstmann S, von Reitzenstein C, Rump I, Kirsch K.Eventing is an Olympic equestrian discipline comprising dressage, cross-country, and show jumping, with the cross-country phase imposing the greatest physical demands on horses. This study presents a composite model to estimate energy expenditure during the cross-country phase, integrating physiological data (heart rate-derived VO2 and lactate-based anaerobic estimates) with external workload indicators (GPS-derived speed, elevation, and course complexity). Model development was based on 691 rides from 256 horses across 232 events at 2-star to 5-star competition levels. The analysis showed tha...
Parra-Moyano LA, Cedeño A, Darby S, Johnson JP, Gomez DE.The effectiveness of the peritoneal fluid L-lactate-to-total solids ratio (PFL:PFTS) as a diagnostic marker for strangulating lesions of the small intestine (SI) and large colon (LC) has not been investigated. Objective: Describe and compare the PFL:PTFS and blood lactate (BL), peritoneal fluid lactate (PFL) and PFL:BL difference and PFL:BL ratio of horses with SI and LC strangulating (SO) and non-strangulating (NSO) obstructions and determine sensitivity and specificity to predict SO. Methods: A total of 282 horses, 117 with SI lesions (59 classified as SINSO and 58 as SISO), and 165 with LC ...
Wright JP, Gray SM, Bishop RC.To describe and demonstrate the collection of abdominal fluid from a standing, sedated equine patient. Unassigned: 1 healthy university-owned animal was used for abdominocentesis demonstration. Images from various clinical cases from the University of Illinois Veterinary Teaching Hospital are shown. Unassigned: Under standing sedation, a 10 X 10-cm area is clipped and aseptically prepared on the most dependent portion of the abdomen to the right of midline. One to 2 mL of 2% lidocaine is instilled subcutaneously and into the abdominal musculature at the abdominocentesis site. A stab incision i...
Macleod BM, Wilkins PA, McCoy AM, Bishop RC.Viscoelastic coagulation testing (VCT) identifies subclinical disruption of coagulation homeostasis and may improve prognostication, particularly for patients with severe systemic inflammation or shock. Machine learning (ML) algorithms may capture complex relationships between clinical variables better than linear regression (GLM). Objective: To evaluate the utility of ML models incorporating VCT and clinical data to predict survival outcomes in horses with acute abdominal pain. Methods: Retrospective observational cohort study. Methods: VCT (VCM Vet™) was performed on 57 horses with acute a...
Garcia LL, Vicentini YF, Nagashima JK, de Souza AF, Pereira MAA, da Silva LCLC, Fantoni DT.To assess the sublingual microcirculation in horses anesthetized with isoflurane and undergoing constant rate infusion of dexmedetomidine (DEX) and lidocaine (LID). Unassigned: 16 horses undergoing arthroscopy were included in a blind, randomized study comparing 2 groups: a DEX group (DEX-LID; 1.75 μg·kg-1·h-1 with a LID bolus of 1.3 mg·kg-1 followed by a continuous infusion of 0.05 mg·kg-1·min-1) and a LID-only group (LID; at the same dose). Hemodynamic variables, including mean, systolic, and diastolic blood pressure; heart rate; capnography; blood gas analysis; blood lactate; and orth...
Davie A, Beavers R, Denham J.The purpose of this review was to analyze the scientific background and practical application of a very successful human training methodology, the pyramidal training model, to the Thoroughbred racehorse. Despite years of research and accumulated knowledge on human training methods to enhance endurance performance, Thoroughbred training programs have lacked significant change. This review introduces the pyramidal training model, explains the science that underpins this training concept and outlines one approach to translate this science into the equine field. It also discusses the importance of...
Van den Branden E, Salamone M, Broothaers K, Peere S, Polfliet E, Dewulf M, Van Steenkiste G, van Loon G, Smits K, Govaere J.The use of transvaginal ultrasound-guided follicle aspiration (TVA) for oocyte collection has become a widely used procedure in horses for embryo production (IVEP). The TVA procedure is characterized by various manipulations, which are physical restraint, perineal preparation, transrectal palpation, insertion of an intravaginal device, and needle punctures. These repeated transvaginal and transovarian punctures have raised concerns about their potential effects on mare welfare. Our study aimed to investigate the effects of TVA manipulations, and especially puncturing, on pain and stress in ma...
Żak-Bochenek A, Drábková Z, Sergedaite V, Siwińska N, Bajzert J, Pasak D, Chełmońska-Soyta A.Colic-related obstructions can reduced intestinal mucosa function and cause dysbiosis in horses, but it is unclear how defense barrier and secretory immunoglobulin A (SIgA) secretion is disrupted. Objective: The aim of the study is to evaluate the effect of severity of colic signs and treatments on fecal SIgA and fecal lactate in horses. Methods: Sixty-two client owned hospitalised horses with colic and eight healthy horses. Methods: Prospective clinical trial. Fecal samples were taken daily for 7 days. SIgA was analyzed using ELISA, and D/L-lactate measured with a commercial kit. Results: A...
Slavik K, Bender S, Sharkey L, Nolen-Walston R.Renal lipidosis is a well-documented histologic finding in humans and small animals with renal and metabolic disorders, but it is not well described in equids. Objective: To describe the signalment, clinicopathologic indices, and postmortem findings of equids with a histologic diagnosis of both hepatic and renal lipidosis (HL + RL) and compare them to cases with hepatic lipidosis only (HL). Methods: Equids with necropsy findings of renal or hepatic lipidosis (state diagnostic lab) between 2008 and 2022. Methods: Retrospective case-control study. Signalment, history, necropsy diagnosis, and...
Wilkins PA, Wong D, Slovis NM, Collins N, Barr BS, MacKenzie C, De Solis CN, Castagnetti C, Mariella J, Burns T, Perkins G, Delvescovo B, Sanchez LC....Sepsis has been defined in humans as the concurrent proven or suspected presence of microbial infection and the systemic inflammatory response syndrome (SIRS). Sepsis is the leading cause of morbidity and mortality in neonatal foals. The clinical utility of using SIRS or its individual components to predict infection and mortality in critically ill foals is currently unknown. Objective: Assess the ability of history and signalment, clinical findings, laboratory results, and SIRS-related indices to predict infection and mortality in critically ill foals. Methods: Retrospective, multi-center, cr...
Carvalho JRG, Sales NAA, Littiere TO, Costa GB, Castro CM, Polisel EEC, Orsi JB, Ramos GV, Santos IFC, Gobatto CA, Manchado-Gobatto FB, Ferraz GC.Several studies have explored alternatives to enhance the performance, health, and safety of sports horses. One promising method involves the use of vibrating platforms (VP), which offer passive exercise stimulation via mechanical oscillations distributed throughout the body. This type of exercise is referred to as whole-body vibration (WBV) and is an emerging strategy for accelerating muscle recovery. This study examined the dynamics of proteins responsible for transporting monocarboxylates (MCT1 and MCT4), and their relationship with lactatemia and acid-base balance in connection with WBV re...
Röschmann J, Naef J, Doras C, Gerber V.Equine asthma is a common cause of poor performance, but little is known about its impact on submaximal exercise. The aim of the present study was to evaluate the effects of equine asthma and potential confounding factors on recovery of respiratory rate (RR) and heart rate (HR) after a lunging exercise test. Thirty-seven horses completed a standardized 15 min lunging exercise including HR and RR recording pre- and up to 30 min post-exercise and venous blood lactate measurement. Endoscopy was performed and bronchoalveolar lavage fluid cytology was used to categorize horses as controls or asthma...
Navas de Solis C, Ramseyer A, Stefanovski D, Haughan J, Solomon CJ, Kirsch K.Exercising arrhythmias are common in horses participating in eventing competitions. Heart rate variability (HRV) and a specific measure of the degree of heart rate fluctuation (Detrended Fluctuation Analysis alpha1; DFA-α1) are proposed as exercise intensity and fatigue markers. Objective: (1) To describe exercising arrhythmias and DFA-α1 values during 2-4* level eventing cross-country competitions in horses from two European countries; (2) to identify associations between exercising arrhythmias, DFA-α1 and competition results and (3) to evaluate whether markers of the intensity of exercise...
Littiere TO, Costa GB, Sales NAA, Carvalho JRG, Rodrigues IDM, Ramos GV, Ferraz GC.In humans, there is an ongoing academic discussion about measuring plasma lactate concentration ([La]) during an incremental exercise test (IET) to establish thresholds that can predict the maximal lactate steady state (MLSS). Training studies on horses have utilized the onset of [La] (OBLA), also known as V, the velocity at which the [La] of 4 mM is reached. This study assesses the effectiveness of four methods for estimating the MLSS in teaching horses. Ten teaching horses underwent a single IET to determine four exercise intensity thresholds for predicting MLSS and performed several constan...
Siegers EW, Parmentier JIM, Sloet van Oldruitenborgh-Oosterbaan MM, Munsters CCBM, Serra Bragança FM.Appropriate training is essential for equine athletes to improve fitness and ensure welfare. Young Friesian stallions must complete a 10-week training program for acceptance as breeding stallions. Earlier, this training program was demonstrated to induce overtraining. Unassigned: To evaluate how this training program affects stallions' trot locomotion variables in relation to fatigue. Unassigned: Prospective analytical study. Unassigned: 3 or 4 years-old (n = 16) Friesian stallions performed three ridden indoor standardized exercise tests (SETs) in week-1 (SET-I; n = 15), week-6 (SET...
Lindqvist A, Nyman G, Rydén A, Wattle O.This study aimed to assess and compare the recovery of ponies and horses following general anesthesia in two different settings: a recovery box with an inflexible, adjustable ceiling, and free recovery without restraints. Our primary objective was to evaluate the effect of adjustable ceilings on the prevention of premature attempts to rise during recovery. The secondary aim was to compare the physiological stress indicators during recovery. Methods: Prospective, randomized, experimental study. Methods: Six healthy ponies and 10 healthy horses. Methods: This study used a crossover design with t...
Martin E, Sarkan K, Viall A, Hostetter S, Epstein K.Neutrophil characteristics in peritoneal fluid (PF) may aid in diagnosing and treating specific colic lesions and complications. The objective of this retrospective study was to evaluate quantitative PF leukocyte values, as well as PF total protein (TP) and lactate, for associations with diagnosis, morbidity, and mortality in horses with acute colic. Three hundred and forty-two horses that presented to one institution between January 2010-2020 for the evaluation of acute colic were included. The PF total nucleated cell count (TNCC), % and total neutrophil counts, total protein (TP), and lactat...
Castelain DL, Dufourni A, Pas ML, Bokma J, de Bruijn E, Paulussen E, Lefère L, van Loon G, Pardon B.The care of sick neonatal foals is labor-intensive and costly. Prediction of risk of death upon admission is often difficult but might support decision-making. Objective: To determine diseases and risk factors associated with death in neonatal hospitalized foals. Methods: Two hundred twenty-two hospitalized foals, ≤7 days old. Methods: Retrospective cohort study. Clinical and laboratory variables were evaluated for their association with death by means of Cox survival analysis and by classification and regression tree (CART) analysis. Results: Most prevalent diseases were sepsis (43.9%), e...
Davis MS, Bayly WM, Hansen CM, Barrett MR, Blake CA.The intracellular environment of skeletal muscle can develop pronounced hyperthermia and acidosis during strenuous exercise, and these alterations in the typical intracellular conditions have been shown to alter mitochondrial respiration. However, the impact of these conditions on ATP synthesis is poorly understood. We used Thoroughbred racehorses to test the hypothesis that both hyperthermia and acidosis decrease the rate of ATP synthesis, but that athletic conditioning mitigates this loss of phosphorylation capacity. Isolated mitochondria were harvested from skeletal muscle before and after ...
Herbst AC, Puglia AT, Malinowski K, McKeever KH.The objective of this study was to determine if calcium propionate is an alkalizing agent in exercising Standardbreds and if it alters plasma glucose and serum insulin concentrations. This study used a randomized crossover design to test the hypotheses that calcium propionate alters total CO2 (tCO), Ca, pH, strong ion difference (SID), glucose, and insulin in Standardbreds completing a simulated race test (SRT) on a high-speed equine treadmill. Blood was collected from eight horses (mean age ± SD = 16 ± 2.7 years; range = 13 - 21 years) at 10 and 5 min prior to treatment or control administr...
Daly K, Proudman CJ, Duncan SH, Flint HJ, Dyer J, Shirazi-Beechey SP.We aimed to determine the effects of variations in dietary composition on equine gut microbiota and their fermentation products, and proposed that dietary modifications profoundly affect microbial ecosystems and their metabolites. Bacterial communities within the large intestine of three groups of horses were compared using oligonucleotide-RNA hybridisation methodology. Each group consisting of six horses was maintained on (1) a grass-only diet, (2) a concentrate diet (i.e. supplemented with hydrolysable carbohydrates) and (3) a concentrate diet but horses were affected by simple colonic obstr...
Biddle AS, Black SJ, Blanchard JL.Laminitis is a chronic, crippling disease triggered by the sudden influx of dietary starch. Starch reaches the hindgut resulting in enrichment of lactic acid bacteria, lactate accumulation, and acidification of the gut contents. Bacterial products enter the bloodstream and precipitate systemic inflammation. Hindgut lactate levels are normally low because specific bacterial groups convert lactate to short chain fatty acids. Why this mechanism fails when lactate levels rapidly rise, and why some hindgut communities can recover is unknown. Fecal samples from three adult horses eating identical di...
Milinovich GJ, Burrell PC, Pollitt CC, Klieve AV, Blackall LL, Ouwerkerk D, Woodland E, Trott DJ.Alimentary carbohydrate overload is a significant cause of laminitis in horses and is correlated with drastic shifts in the composition of hindgut microbiota. Equine hindgut streptococcal species (EHSS), predominantly Streptococcus lutetiensis, have been shown to be the most common microorganisms culturable from the equine caecum prior to the onset of laminitis. However, the inherent biases of culture-based methods are estimated to preclude up to 70% of the normal caecal microbiota. The objective of this study was to evaluate bacterial population shifts occurring in the equine caecum throughou...
Darr CR, Varner DD, Teague S, Cortopassi GA, Datta S, Meyers SA.Stallion sperm rely primarily on oxidative phosphorylation for production of ATP used in sperm motility and metabolism. The objective of the study was to identify which substrates included in Biggers, Whitten, and Whittingham (BWW) media are key to optimal mitochondrial function through measurements of sperm motility parameters, mitochondrial oxygen consumption, and cellular reactive oxygen species (ROS) production. It was expected that mitochondrial substrates, pyruvate and lactate, would support sperm motility and mitochondrial function better than the glycolytic substrate, glucose, due to d...
Genzel Y, Behrendt I, König S, Sann H, Reichl U.The production of equine influenza in Madin-Darby canine kidney (MDCK) cells in large-scale microcarrier culture is described with detailed on- and off-line analytical data during cell growth and virus replication. Metabolite concentration profiles for glucose, glutamine, lactate and ammonium are shown. Lactate and ammonium concentrations were always below inhibiting levels. Concentration profiles for essential and non-essential amino acids of the cell culture medium are discussed. During cell growth proline was released into the medium with a significant rate while two amino acids, serine and...
Witkowska-Piłaszewicz O, Maśko M, Domino M, Winnicka A.In horse racing the most acceptable way to objectively evaluate adaptation to increased exertion is to measure lactate blood concentration. However, this may be stressful for the horse, therefore, a simple, noninvasive procedure to monitor race progress is desirable. Forty Thoroughbreds attended race training, with blood samples collected at rest, immediately after, and 30 min after exercise. The lactate concentration was determined 60 s after blood collection using an Accusport. Thermal imaging of the neck and trunk areas was performed following international veterinary standards from a dista...
Corley KT, Donaldson LL, Furr MO.Blood lactate concentration has been shown to be a useful clinical indicator in human patients, but has not been formally investigated in critically ill foals. Objective: To investigate the association of blood lactate with hospital survival, markers of cardiovascular status, metabolic acid base status, sepsis and systemic inflammatory response syndrome (SIRS). Methods: A database containing clinical, haematological, plasma biochemical and hospital outcome data on neonatal foals referred to an intensive care unit in 2000-2001 was analysed. Seventy-two foals for which arterial lactate was measu...
Byrd SK, McCutcheon LJ, Hodgson DR, Gollnick PD.This study examined the effects of acute high-intensity exercise on the rate and capacity of Ca2+ uptake and Ca2+-stimulated adenosinetriphosphatase (ATPase) activity of the sarcoplasmic reticulum and the reversibility of these effects. Thoroughbred horses were run at maximal O2 uptake on a high-speed treadmill until fatigued. Muscle temperatures and biopsy samples were collected at rest, immediately after exercise, and 30 and 60 min after exercise. Blood samples were collected at rest and 5 min after exercise. Muscle and blood (lactate concentration) were three- and fivefold greater than pre-...
Lacitignola L, Fanizzi FP, Francioso E, Crovace A.Proton magnetic resonance spectroscopy (1H NMR) has been successfully used in the study of many biological fluids. The data presented here report on the metabolic profiles of normal equine synovial fluids compared with osteoarthritic (OA) fluids. Twenty-five OA synovial fluid samples and eight normal ones were collected from the forelimb fetlock joint in 22 horses, aged between five and 24 years. 1H NMR spectroscopy was carried out with a Bruker Avance DRX 500 equiped with a cryo-magnet working at 11 Tesla, and 'Mestre-C 4.9.9.6' software was used to analyze the spectra. The study assessed the...
Rivero JL, Ruz A, Martí-Korff S, Estepa JC, Aguilera-Tejero E, Werkman J, Sobotta M, Lindner A.This study examined the effects of the intensity and duration of exercise on the nature and magnitude of training adaptations in muscle of adolescent (2-3 yr old) racehorses. Six thoroughbreds that had been pretrained for 2 mo performed six consecutive conditioning programs of varying lactate-guided intensities [velocities eliciting blood lactate concentrations of 2.5 mmol/l (v2.5) and 4 mmol/l (v4), respectively] and durations (5, 15, 25 min). Pre- and posttraining gluteus muscle biopsies were analyzed for myosin heavy chain content, fiber-type composition, fiber size, capillarization, and fi...
Munsters CC, van Iwaarden A, van Weeren R, Sloet van Oldruitenborgh-Oosterbaan MM.Regular exercise testing in Warmblood sport horses may, as in racing, potentially help to characterise fitness indices in different disciplines and at various competition levels and assist in understanding when a horse is 'fit to compete'. In this review an overview is given of the current state of the art of exercise testing in the Olympic disciplines of eventing, show jumping and dressage, and areas for further development are defined. In event horses, a simple four-step incremental exercise test measuring heart rate (HR), lactate concentration (LA) and velocity (V) is most often used. In dr...
Al Jassim RA, Scott PT, Trebbin AL, Trott D, Pollitt CC.Seventy-two lactic acid producing bacterial isolates (excluding streptococci) were cultured from the gastrointestinal tract of six horses. Two of the horses were orally dosed with raftilose to induce lactic acidosis and laminitis while the remaining four were maintained on a roughage diet. Near complete 16S rDNA was amplified by PCR from the genomic DNA of each isolate. Following RFLP analysis with the restriction enzymes MboI, HhaI and HinfI, the PCR products from the 18 isolates that produced L- and/or D-lactate were subsequently cloned and sequenced. DNA sequence analysis indicated that the...
Balogh N, Gaál T, Ribiczeyné PS, Petri A.Physical exercise in the horse induces a series of normal physiological and biochemical adaptations. Increasing metabolism and oxygen uptake may induce oxidative stress in various organs. The aim of this study was to examine exercise-induced changes in some plasma and RBC biochemical and antioxidant variables in pentathlon horses. Blood samples were taken from 14 horses before, immediately after, and 24 hours after competing in two 1-minute runs of intense exercise over jumps. The peak intensity periods were preceded by a 20-minute warm-up and separated by a 20-minute break. The following plas...
Johnston K, Holcombe SJ, Hauptman JG.To determine the relationship between plasma lactate concentration and colonic viability and survival in horses with >or=360 degrees volvulus of the ascending colon. Methods: Retrospective study. Methods: Horses (n=73) with >or=360 degrees volvulus of the ascending colon. Methods: Medical records (January 2000-November 2005) of all horses examined for colic at Michigan State University Veterinary Teaching Hospital were reviewed. Horses were included only if plasma lactate concentration was measured preoperatively and a diagnosis of >or=360 degrees volvulus of the ascending colon was c...
Le Moyec L, Robert C, Triba MN, Billat VL, Mata X, Schibler L, Barrey E.During long distance endurance races, horses undergo high physiological and metabolic stresses. The adaptation processes involve the modulation of the energetic pathways in order to meet the energy demand. The aims were to evaluate the effects of long endurance exercise on the plasma metabolomic profiles and to investigate the relationships with the individual horse performances. The metabolomic profiles of the horses were analyzed using the non-dedicated methodology, NMR spectroscopy and statistical multivariate analysis. The advantage of this method is to investigate several metabolomic path...
Couëtil LL, Denicola DB.The purpose of this study was to evaluate the relationship between bronchoalveolar lavage (BAL) cytology, arterial blood gases and plasma lactate concentration during a standardised treadmill test (STT) in racehorses with small-airway inflammation (SAI), or exercise-induced pulmonary haemorrhage (EIPH). Sixteen Thoroughbred and 20 Standardbred actively racing racehorses, were divided into a control group (n = 10), EIPH group (n = 13) and SAI group (n = 13). Each STT consisted of a 2 min trot at 4 m/s followed by 5 x 1 min, incremental speed steps (6, 8, 10, 11 and 12 m/s) at 10% incline for Th...
Castagnetti C, Pirrone A, Mariella J, Mari G.The use of blood lactate concentration as an indicator of prognosis and disease severity has become a common practice in equine medicine, especially with the validation of handheld analyzers. However, few authors described lactate concentration in critically ill foals, and there are no published studies about the use of handheld analyzers in neonatal foals. In this study, for the first time in the equine neonate, we validated the Lactate Scout analyzer, both in healthy and in critically ill foals. The study also describes the normal range for blood lactate in 26 healthy neonatal foals during t...
Latson KM, Nieto JE, Beldomenico PM, Snyder JR.The most common cause of death as a direct result of colic is acute circulatory failure secondary to intestinal ischaemia. Early and accurate recognition of ischaemic bowel is essential to decrease complications and increase survival. Blood to peritoneal lactate values have been evaluated as a prognostic indicator, but lactate values characterised by type of lesion have not been reported. Objective: Plasma and peritoneal lactate values are higher in horses with intestinal ischaemia secondary to a strangulating obstruction (ISSO). Methods: Venous blood and peritoneal fluid were collected sequen...
Bukhari SSUH, McElligott AG, Parkes RSV.There are approximately 112 million working equids in developing countries, many of which are associated with brick kilns. Brick kilns and overloading are associated with welfare problems in working equids. Understanding equids' abilities and influencing factors are important for both effective performance and welfare. Traditionally, measurement of the amount of 'bone' was used, and more recently, gait symmetry has been identified as a potential marker for loading capacity. Assessment of stride parameters and gait kinematics provides insights into adaptations to loading and may help determine ...
Speake PF, Roberts CA, Gibson JS.K influx into equine red blood cells (RBCs) was measured using 86Rb as a tracer for K under conditions designed to mimic the changes in respiratory blood parameters that occur in vivo during strenuous exercise. The effects on K influx of physiological changes in pH, cell volume, O2 tension (PO2), CO2 tension (PCO2), and bicarbonate and lactate concentrations were defined. Physiological PO2 exerted a dominant controlling influence on the H(+)-stimulated Cl-dependent K influx, consistent with effects on the K-Cl cotransporter, PO2 required for half-maximal activity was 37 +/- 3 mmHg (4.9 kPa). A...
Evans DL, Harris RC, Snow DH.At 2 and 5 mins after an 800-m gallop, venous blood was collected from 26 Thoroughbred racehorses for measurement of blood lactate concentration, packed cell volume (PCV) and haemoglobin concentration. In addition, 14 racehorses were given a strenuous submaximal treadmill exercise test. Heart rates during and after exercise at 10 m/sec on a treadmill inclined at 5 degrees were recorded. Blood samples at 2 and 5 mins after exercise were used to measure PCV, blood and plasma lactate and ammonia concentrations. Results of each exercise test were compared with the retrospective performance of hors...
Tennent-Brown BS, Wilkins PA, Lindborg S, Russell G, Boston RC.Sequential lactate concentration ([LAC]) measurements have prognostic value in that hospitalized humans and neonatal foals that have a delayed return to normolactatemia have greater morbidity and case fatality rate. Objective: Prognosis for survival is decreased in horses with a delayed return to normal [LAC]. Methods: Two hundred and fifty adult horses presented for emergency evaluation excepting horses evaluated because of only ophthalmologic conditions, superficial wounds, and septic synovitis without systemic involvement. Methods: Prospective observational study. [LAC] was measured at admi...
McGowan C.Clinical pathology is frequently used in racehorses to screen for "fitness" to race and to screen for disease that may affect performance. The role of clinical pathology in exercise testing of racehorses is important, especially in the area of blood lactate responses to exercise. This article discusses the use of resting and dynamic clinical pathology in the racehorse, especially in respect to investigation of the state of training (or fitness) and detection of subclinical performance-limiting disease.
Jansson A, Lindberg JE.Most athletic horses are fed a high-starch diet despite the risk of health problems. Replacing starch concentrate with high-energy forage would alleviate these health problems, but could result in a shift in major substrates for muscle energy supply from glucose to short-chain fatty acids (SCFA) due to more hindgut fermentation of fibre. Dietary fat inclusion has previously been shown to promote aerobic energy supply during exercise, but the contribution of SCFA to exercise metabolism has received little attention. This study compared metabolic response with exercise and lactate threshold (VLa...
Rivero JL, Hill EW.Skeletal muscles in horses are characterised by specific adaptations, which are the result of the natural evolution of the horse as a grazing animal, centuries of selective breeding and the adaptability of this tissue in response to training. These adaptations include an increased muscle mass relative to body weight, a great locomotor efficiency based upon an admirable muscle-tendon architectural design and an adaptable fibre-type composition with intrinsic shortening velocities greater than would be predicted from an animal of comparable body size. Furthermore, equine skeletal muscles have a ...
Armstrong RB, Essén-Gustavsson B, Hoppeler H, Jones JH, Kayar SR, Laughlin MH, Lindholm A, Longworth KE, Taylor CR, Weibel ER.The purpose of this study was to describe the relationships between 16 physiological, biochemical, and morphological variables presumed to relate to the oxidative capacity in quadriceps muscles or muscle parts in Standardbred horses. The variables included O2 delivery (blood flow) and mean capillary transit time (MTT) during treadmill locomotion at whole animal maximal O2 consumption (VO2max, 134 +/- 2 ml.min-1 x kg-1), capillary density and capillary-to-fiber ratio, myoglobin concentration, oxidative enzyme activities, glycolytic enzyme activities, fiber type populations, and fiber size. Thes...
Morita H, Nakano A, Shimazu M, Toh H, Nakajima F, Nagayama M, Hisamatsu S, Kato Y, Takagi M, Takami H, Akita H, Matsumoto M, Masaoka T, Murakami M.To detect the predominant lactobacilli in the intestinal flora of healthy thoroughbreds, we isolated lactobacilli from the feces of nine thoroughbreds (five males and four females; 0-15-year-old). The isolated lactobacilli comprise 17 species (37 strains), and they were classified into five groups: Lactobacillus salivarius (6 species), L. reuteri (6 species), Lactobacillus delbrueckii (3 species), L. buchneri (1 species) and L. vitulinus (1 species). On the basis of 16S rRNA gene sequences, we identified 3 other phylogenetic relatives belonging to the genus Lactobacillus. These results suggest...
Maśko M, Domino M, Jasiński T, Witkowska-Piłaszewicz O.Blood testing is one of the most important ways to improve performance, facilitate recovery and monitor the training of endurance and race horses. However, little is known about the physical activity-dependent changes of blood parameters in horses used for pleasure and in riding schools. This study aimed to perform routine blood tests for training monitoring of sport horses in three different horse types of use. Then the values of blood indicators were compared between school, endurance and race horses to find similarities in the physical activity-dependent profile. The study was carried out o...
Nadeau JA, Andrews FM, Mathew AG, Argenzio RA, Blackford JT, Sohtell M, Saxton AM.To measure pH, volatile fatty acid (VFA) concentrations, and lactate concentrations in stomach contents and determine number and severity of gastric lesions in horses fed bromegrass hay and alfalfa hay-grain diets. Methods: Six 7-year-old horses. Methods: A gastric cannula was inserted in each horse. Horses were fed each diet, using a randomized crossover design. Stomach contents were collected immediately after feeding and 1, 2, 3, 4, 5, 6, 7, 8, 10, 12, and 24 hours after feeding on day 14. The pH and VFA and lactate concentrations were measured in gastric juice Number and severity of gastri...
