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Topic:Observational Study
Observational studies in horses involve systematically assessing and recording behaviors, conditions, or other characteristics without manipulating the study environment. These studies provide insights into natural equine behaviors, health conditions, and interactions with their environment. Researchers often focus on aspects such as herd dynamics, feeding patterns, or responses to environmental changes. The data collected can help identify trends, correlations, and potential risk factors for certain conditions. This page compiles peer-reviewed research studies and scholarly articles that explore various observational methodologies and their applications in understanding equine behavior and health.
Validation of an improved insect bite hypersensitivity severity score for allergic equine insect bite hypersensitivity in horses. Insect bite hypersensitivity (IBH) is the most common allergic skin disease in horses, but existing clinical scoring systems lack extensive validation, limiting standardized disease monitoring and therapeutic evaluation. Objective: Develop and validate a standardized, sensitive, and reproducible clinical scoring system for IBH in horses. Methods: Forty-four privately-owned horses with clinically diagnosed IBH examined under field conditions in Switzerland. Methods: In this prospective field validation study, the equine IBH severity score (EqIS) integrates a lesion severity score and an area sc...
Behaviour and Movement Activity of Stallions and Geldings in Group Housing. There has been little research on the social behaviour of stallions and geldings kept together in group housing systems. The aim of this study was to investigate differences in behaviour and movement activity between stallions and geldings kept together in group housing. Behavioural observations and GPS measurements were conducted in Germany from July to September 2024 in four mixed groups of horses in Germany. The groups comprised 35 animals, 18 stallions and 17 geldings. The horses were observed for three consecutive days over eight hours per day. No injuries or stereotypic behaviours were o...
Prevalence and radiographic features of cyst-like lesions in German Warmblood horses presented for pre-purchase examination. Cyst-like lesions (CLLs) are frequently detected during radiographic examinations of equine limbs. They may represent a potential risk factor during pre-purchase examination (PPE) and are of considerable clinical and economic importance, as they may lead to negative purchase decisions. While affected horses may develop lameness, incidental findings have also been reported. The goal of the study was to describe the prevalence, location and radiographic characteristics of CLLs in a group of sound German Warmblood horses. Unassigned: A retrospective cohort study was conducted on 3863 German Warmb...
Determining optimal practices for foal weaning – A protocol for a systematic review and network meta-analysis. Weaning is a stressful time in a foal's (Equus caballus) life. As humans artificially manage weaning in domestic horses, it provides an opportunity to safeguard horse welfare early in life, with potential long-term benefits. While numerous studies have examined the effects of different weaning and management interventions on foal stress, optimal weaning practices have yet to be identified. Overall purpose of the study: To identify which weaning interventions and management practices have the greatest potential to improve foal welfare based on physiological and behavioural outcomes. Research qu...
Risk factors for post-partum colic in a population of UK and Irish Thoroughbred broodmares. Broodmares are at increased risk of gastrointestinal colic. However, colic risk factors specific to this population have not been investigated. Objective: To investigate horse- and management-level risk factors for post-partum colic in a cohort of Thoroughbred broodmares in the United Kingdom and Ireland. Methods: Prospective, nested case-control study. Methods: Twenty-nine Thoroughbred stud farms in the United Kingdom and Ireland were recruited, and 1044 mares were followed over the 2021 and/or 2022 foaling seasons. Data were collected on signalment, medical and management history for post-pa...
Inertial measurement units are comparable to motion capture for measuring intersegmental spinal angular velocities in horses. To compare inertial measurement unit (IMU)-derived intersegmental spinal motion data with measurements obtained from a marker-based motion-capture system. Unassigned: 4 horses were instrumented with custom marker clusters incorporating a 3-D-printed base designed to secure the IMU, ensuring both systems recorded data from the same anatomical location and orientation. A total of 3,737 paired time points across 37 gait cycles were analyzed. Agreement in intersegmental angular velocities across 3 planes of motion was evaluated using Bland-Altman analysis and intraclass correlation coefficients. U...
Fatalities within seven days after equine standing sedation: A prospective, multicentre study. Information regarding mortality associated with standing procedures in horses is sparse. Objective: To report data from >12,000 standing sedations. Methods: Confidential, observational, prospective, multicentre, cohort study. Methods: We collected records of horses/ponies undergoing standing sedation for surgery or advanced diagnostic imaging using at least one top-up or controlled rate infusion (CRI). Procedures were either NON-COLIC or COLIC. Outcome at seven days was recorded as (i) ALIVE, (ii) EUTHANASIA or (iii) DEAD. Data were collected with a portable document format (PDF) questionn...
Effect of exercise-related nasopharyngeal disorders on upper airway function in racehorses. Exercise-related upper respiratory tract (URT) disorders are frequently diagnosed in racehorses. Treatment recommendations are routinely based on subjective assessment of clinical evaluation and exercising endoscopy findings. Objective: To objectively investigate how palatal instability (PI), intermittent dorsal displacement of the soft palate (iDDSP), alar fold collapse (AFC), and nasopharyngeal collapse (NPC) affect upper airway function during exercise in racehorses. Methods: Observational study with case-control comparisons. Methods: Inspiratory and expiratory tracheal pressures (cmHO) wer...
Advanced paternal age is associated with reduced reproductive success and offspring racing performance in Australian Thoroughbred racehorses. Reproductive ageing influences fertility and offspring fitness across mammals. In humans and model organisms, paternal age has been associated with changes in sperm quality and genomic integrity, including oxidative stress, DNA damage, and epigenetic alterations; however, these mechanisms have not been directly examined in large equine populations. Thoroughbred racehorses provide a system to investigate paternal age effects at scale using detailed breeding records. In this retrospective observational study, with data from 35 retired stallions and 33,546 progeny, we assessed associations betwee...
Phenotypic characterization through identification of gait and coat color phenotype of the Mountain Pleasure Horse. Phenotypic characterization of traits like gait and coat color is useful for minor breed identification and conservation. The Mountain Pleasure Horse (MPH) is a minor American breed as it is listed as threatened by the Livestock Conservancy. Objective: The study objective was to utilize phenotypic characterization for identifying gait and coat color phenotype of the MPH. Methods: Gait was characterized utilizing frame-by-frame analysis to identify spatiotemporal variables of purebred horses (n = 6) performing the flat walk and saddle rack. Coat color was characterized using coat color assi...
Mastication characterization of horses fed bermudagrass hay in a two-part field study. Bermudagrass (Cynodon dactylon L.) is a common forage in the southeastern United States but remains controversial in equine diets due to reported associations with impaction colic. Reduced fiber digestibility has been proposed as a contributing factor, however differences in mastication of fine-stemmed bermudagrass hay have not been studied and may play a role in digestive disturbances. Objective: To compare chewing characteristics, including chews per unit weight and chews per minute, in horses consuming bermudagrass hay compared to other common hay types. Methods: Two studies were conducted....
Rider dislodgement events in U.S. steeplechase racing: participation outcomes in riders and horses. Steeplechase jockeys compete in high-risk races requiring horses and riders to clear fixed obstacles at speed. Although rider dislodgement events are recognized hazards, their incidence and participation consequences are not well characterized. Methods: This retrospective observational study used publicly available race charts from United States National Steeplechase Association races (2023-2025). Dislodgement events were defined as falls or lost rider events. Incidence rates were calculated per 1,000 starts, and timber and hurdle races were compared using Poisson regression. Rider interruptio...
Musculoskeletal Burden in German Equestrians: Hip-Specific Symptoms and Risk Factors. Equestrian sport is a major component of German athletics, with broad recreational participation and a strong competitive sector. In 2019, about 2.32 million people identified as active riders, and more than 60,000 competitional licences were issued in 2021, reflecting substantial competitive engagement across the Olympic disciplines and national performance levels. Hip pain is a frequent and clinically relevant complaint among athletes and can stem from structural, degenerative, or overuse-related causes. In riding, the hip joint is crucial for transmitting forces and coordinating rider-horse...
A study of the forces generated during equine cheek tooth extraction. Significant force is often applied when elevating equine teeth from the alveolus during extraction. No previous studies have investigated these forces. Objective: To develop a method to allow measurement of elevation forces (EF) applied during extraction, and to record forces during extraction of cheek teeth in both cadaver specimens and clinical cases, evaluating factors that might affect this and comparing differences between tooth positions and horse signalments. Methods: In vitro experimental study of cadavers and observational study of a convenience sample of clinical cases. Methods: Stra...
Evaluation of Point-of-Care Coagulation Measurement Systems for Activated Clotting Time and Partial Thromboplastin Time in Heparinized and Non-Heparinized Equine Blood. To compare the performance of one point-of-care activated clotting time (ACT) machine (Vet-Tube), one point-of-care activated partial thromboplastin time (aPTT) machine (Coagulation DX), and one bench-top ACT machine (Medtronic ACT II plus) with previously published measurements from stationary devices in whole blood from healthy horses. Methods: Prospective, observational study SETTING: University veterinary teaching hospital, from August 2023 to September 2023. Methods: Thirty healthy university-owned horses. Methods: ACT and aPTT were measured via point-of-care analyzers (ACT POC, aPTT POC)...
Successful restoration of heathlands and grasslands associated with long-term habitat preferences of cattle and horses: Insights from GPS tracking. Long-term, year-round grazing by large herbivores is increasingly used to restore degraded temperate heathlands and grasslands, yet the behavioral mechanisms of grazers underlying restoration remain insufficiently understood. We combined over 13 years of high-resolution GPS-tracking data of free-ranging Heck cattle and Konik horses with repeated vegetation surveys across various habitat types (encroachment-derived Calamagrostis epigejos stands, dry sandy grasslands, dry heaths, and pioneer forests) in Central Germany. We quantified how habitat preference and vegetation structure co-develop und...
Cervical spinal cord compression may affect anesthetic recovery quality and seizure incidence in horses after myelography. To compare anesthetic recovery quality and seizure incidence after cervical myelography in horses with or without cervical spinal cord compression. Unassigned: Anesthesia and medical records from January 2017 to December 2022 of horses anesthetized for myelography were retrieved. Patients with incomplete records, equine protozoal myeloencephalitis diagnosis, or an anesthetic protocol outside an established standard were excluded. Demographic analysis, ataxia score, and anesthetic management variables were recorded in an observational retrospective fashion. Each variable was compared between gr...
A non-trauma-focused equine-assisted intervention was associated with reductions in all four PTSD symptom clusters in treatment-resistant military veterans. Veterans are at greater risk of developing post-traumatic stress disorder (PTSD) and show a poorer response to first-line treatments than civilians. Although equine-assisted interventions (EAIs) represent a promising alternative for military veterans,the underlying mechanisms remain uncertain, and their effectiveness across different PTSD symptom clusters has yet to be established. This study examined the effects of a non-trauma-focused EAI on specific PTSD symptoms in treatment-resistant military veterans. Unassigned: Participants in the intervention were military veterans diagnosed with PTSD...
Equine-assisted coaching in formerly incarcerated men with histories of substance abuse: a 12-week exploratory study of anger regulation, quality of life, human-horse synchrony, and equine behavior. Equine-assisted interventions are increasingly used as complementary approaches to support psychosocial functioning, yet empirical evidence in justice-involved populations with histories of substance abuse remains limited. The present exploratory quantitative study examined the effects of a 12-week equine-assisted coaching program on anger regulation, quality of life, and human-horse synchrony in formerly incarcerated men, while also monitoring equine behavioral responses from a One Health perspective. Eleven male participants residing in a residential reintegration facility in the Netherlands...
Do Polish Konik Horses Find Soaked Hay to Be Palatable? Frequently, equine diseases necessitate the moistening or soaking of hay for the horses. It was assumed that this treatment would not reduce the willingness of the Polish Konik horses with low nutritional requirements to consume the hay. Furthermore, it was believed that moistening would increase the palatability of this feed. The aim of this study was to determine time and frequency parameters and the amount of unconsumed dry, moist, or soaked hay. The research material comprised 12 Polish Konik horses consuming either 1 kg of dry hay, 2.1 kg of moist hay, or 3.3 kg of soaked hay on three sub...
Feasibility of a hand-held myotonometry device for measuring biomechanical muscle parameters in horses. To evaluate the feasibility of a hand-held myotometry device for measuring the biomechanical parameters (frequency, stiffness, and decrement) of the longissimus dorsi muscle in the thoracolumbar region of healthy horses. Unassigned: Data were obtained from 50 adult horses. The biomechanical parameters were measured using a MyotonPRO device in triple-scan mode. Measurements were recorded on both sides, with the horses standing square. The analysis focused on whether there were differences between the sides and correlations between the variables. Unassigned: The mean muscle frequency (hertz) was...
Anatomical distribution of meconium detected by focused abdominal ultrasound in hospitalized healthy and sick neonatal foals. Meconium impaction is a common cause of neonatal colic in foals, yet its ultrasonographic appearance and distribution remain poorly characterized. Objective: This study aimed to describe the frequency, distribution, and ultrasonographic appearance of meconium in hospitalized neonatal foals using a standardized focused ultrasound (FUS) abdominal protocol. Methods: The FUS protocol included 9 acoustic windows (3 left-sided, 3 ventral, 3 right-sided) and was performed once during hospitalization by a single operator using a curvilinear transducer. Results: Thirty-six foals (16 healthy and 20 sick...
Outcomes and racing performance in thoroughbreds following a long-term injury sustained during a jump race in Great Britain. Limited literature is available about the career outcomes of horses sustaining a long-term injury (LTI) in a jump race. Methods: All Thoroughbreds that sustained an LTI, defined as any non-fatal musculoskeletal injury requiring a minimum 90-day break from racing, in a British jump race between May 2018 and April 2023 were followed up for at least 24 months and outcomes described. Pre-injury racing performance was compared between horses that did and did not return to racing, and post-injury racing performance was compared between horses that sustained tendon/ligament injuries and fractures. R...
Conflict Behaviour Frequency During Show Jumping Competitions: A Practical Study. With society's ever-growing concern for animal welfare, the equestrian industry has focused on passing and enforcing new rules to protect the main athlete, the horse. In jumping, courses go by quickly, with judges using the occurrence of conflict behaviours to assess any welfare breach. This study aimed to evaluate the overall frequency of such behaviours in Brazilian Sport Horses during elite show jumping competitions in Brazil. Conflict behaviour displays were analyzed in 120 different horse-rider pairs in jumping competitions in Brazil. All videos were observed at a slowed-down speed, with ...
Management factors associated with abnormal behaviours in rural Chilean working horses. Working horses remain essential for rural livelihoods in Chile and across Latin America. However, housing and management conditions in these systems are poorly characterised, underscoring the need for standardised welfare assessments to generate evidence and guide targeted improvements. Objective: To estimate the prevalence of abnormal behaviours in rural working horses and to examine associations with management and housing related factors. Methods: Cross-sectional observational study. Methods: Seventy-seven working horses from a rural area of the Papudo commune (Valparaíso Region, Chile) we...
Industry Perceptions of Thoroughbred Racehorse Emotions and Quality of Life: Implications for the Development of an Equine Quality of Life Assessment Tool. Understanding how horses experience their lives is essential for improving their welfare, particularly in high-performance industries such as horseracing. Quality of Life (QoL) frameworks highlight the balance between positive and negative experiences over time, yet there is limited evidence on how racing professionals assess horse emotions and QoL, especially positive emotional states. This study reports findings from an international online survey of 230 racing industry participants. It explored confidence in assessing racehorse emotions, the perceived importance of emotional state for perfo...
Cardiac Cost During Submaximal Exercise as a Practical Monitoring Tool in French Standardbred Trotters: Short-Term Reproducibility of Non-Invasive Field-Derived Indicators. Routine monitoring in racehorses requires indicators that are reproducible and practical under real training conditions. This observational study evaluated the short-term reproducibility of cardiovascular and speed indicators in French Standardbred trotters, with a particular focus on cardiac cost (CC), defined as the ratio of heart rate to speed (beats·m). The full dataset comprised 483 sessions from 60 trotters and was used to describe age-related patterns. For reproducibility analyses, consecutive monitored sessions within the same horse were grouped into follow-up blocks when the interval...
Open habitats and species differences shape space use in semi-feral cattle and horses across Danish rewilding sites. Large herbivores can strongly influence ecosystem structure and biodiversity, making knowledge of their space use important for trophic rewilding. We used GPS-tracking data collected over two years from 10 semi-feral cattle and 17 horses across ten Danish rewilding sites to analyse habitat use in a use-availability framework using resource selection functions (RSFs), complemented by direct behavioural observations. Both species showed large individual variability but overall used habitats disproportionately to availability, with higher relative use of open habitats; including dry grassland and...
Effects of an Equine-Assisted Riding Program on Motor Performance, Movement Quality, and Well-Being Among Young Inmates. Equine-assisted programs have been shown to promote psychosocial outcomes, but quantitative evidence of motor benefits in correctional settings is scarce. Objective: The present study examined the effects of a one-week equine-assisted riding program on riding performance, movement quality, and well-being among young inmates in an open German prison. Methods: Ten male participants (24.5 ± 0.71 years) completed a five-day program combining practical riding exercises, cooperative activities, and guided reflection. Riding performance was assessed using standardized expert video ratings based on t...
