Equine health encompasses the study and management of diseases, disorders, and overall well-being of horses. It involves understanding various physiological systems, preventive care, and treatment strategies to maintain optimal health in equine populations. Common areas of focus include nutrition, infectious diseases, orthopedic conditions, and reproductive health. Research in equine health aims to advance knowledge on diagnostic methods, therapeutic interventions, and management practices that improve horse welfare and performance. This page collects peer-reviewed research studies and scholarly articles that explore the diverse aspects of equine health, offering insights into current findings and advancements in the field.
Watson W, MacKay JRD, Dwyer C.This study aims to improve the welfare and management of recreational horses by identifying how different management styles affect horse health and behaviour. We examined the management styles of recreational horse owners in the UK and Ireland, focusing on social interaction (friends), access to suitable forage (forage), and unrestricted movement (freedom). We collected 1,501 survey responses, distributed via social media, and summarised the characteristics and management choices of the respondents. Using the Divisive ANAlysis cluster package in R, three distinct management styles were identif...
Ledbetter EC, Knickelbein KE, Irby NL, Schmidt C.To describe the clinical and in vivo confocal microscopy (IVCM) characteristics of horses with corneal stromal invasive squamous cell carcinoma (CSI-SCC). Methods: Retrospective study of 7 horses with histopathologically confirmed unilateral CSI-SCC. Methods: Horses with corneal CSI-SCC were examined by laser scanning IVCM. Signalment, clinical findings, and histopathology results were compared with IVCM findings. Results: Clinical ocular lesions in all horses included a heavily vascularized, opaque, gray or tan, anterior or midstromal opacity invading the cornea from the limbus. The corneal ...
Armangau M, Lurier T, Josson A.Offspring Sex Ratio (OSR) at birth in mammals is generally assumed to follow a 1:1 male-to-female distribution, yet deviations in OSR have been observed and may be influenced by maternal and paternal factors. In this study we have focused on the effect of paternal factors (stallion age and breed) and breeding methods on the OSR. Using a retrospective dataset of 251,783 foal births recorded in France between 2015 and 2024, we applied generalized linear mixed models to evaluate the effects of these variables on the likelihood of producing a female foal. Stallion identity was included as a random...
Dillmann JB, Dos Santos TS, Dos Santos RC, Monteiro SG.Equine cutaneous pythiosis, caused by Pythium insidiosum, is a severe disease in tropical and subtropical regions, often leading to progressive granulomatous lesions with poor response to conventional therapies. Standard treatment includes surgical excision and immunotherapy, but recurrence is common, particularly in distal limb lesions. Maggot Debridement Therapy (MDT) with disinfected Lucilia cuprina larvae provides selective debridement, microbial control, and stimulation of granulation, representing a potential adjunctive option. This report describes the successful use of MDT combined wit...
Skierbiszewska K, Turek B, Jasiński T, Kaczorowski M, Kozłowska N, Higuchi J, Domino M.Bone grafting in equine medicine offers a promising contribution to treating orthopaedic developmental diseases and chondral, osteochondral and segmental bone defects. Among grafts, synthetic bone substitutes-alloplastics-show favourable biological properties addressing numerous limitations presented by autografts, xenografts and allografts. Objective: To compile and disseminate clinical data and research findings from existing publications on the use of alloplastics in horses. Methods: Systematic review. Methods: Following Preferred Reporting Items for Systematic Reviews and Meta-analysis 202...
Cottone A, Seiter K, Thomas B, Schank N, Wulf M, Miller L, Anderson S, Munkhsaikhan U, Verma A, Abidi AH, Kassan M.Colic remains one of the most frequent and costly causes of equine morbidity and mortality, with significant welfare and economic implications. Disturbances in the gut microbiome are increasingly recognized as an important contributing factor. In recent years, prebiotics, non-digestible substrates that promote beneficial microbes, have emerged as promising microbiome-targeted strategies. () has gained attention for its unique ability to degrade mucin, maintain epithelial integrity, and exert potent anti-inflammatory effects. Although its benefits are well established in humans and rodent mode...
Chan ZYS, Ho W, Slobodova A, Parkes RSV, Forbes B, Pfau T.To investigate associations between contralateral (left-right) hoof width asymmetry and vertical movement asymmetry in racing Thoroughbreds. We hypothesized that forehoof differences would be associated with poll and withers movement asymmetry and hind hoof differences with pelvis movement asymmetry. Unassigned: This observational cross-sectional study included 169 Thoroughbred racehorses recently retired from clockwise racing and assessed the day of scheduled farriery work. Contralateral hoof width differences were quantified and movement asymmetry during trot measured using inertial sensors ...
Rosa MCB, Campos MEST, Albuquerque AS, Braz MS, Mapele RO, de Oliveira MCG, Toma HS, Ferraz PFP, Pereira RN, Raymundo DL.Skin wounds in horses, particularly in the distal extremities, often heal slowly due to mechanical stress, compromised perfusion, and excessive fibroplasia. This study evaluated the effects of topical Croton lechleri (CL) cream, alone or in combination with nitroglycerin, on the healing of skin wounds in the metatarsal region of horses. Six mares received six standardized wounds in each region and were treated with: saline solution, nitroglycerin (0.1 or 1 mg/mL), CL (1 or 10 µg/mL), or CL + nitroglycerin (10 µg/mL + 1 mg/mL). Wound contraction, histology, and bacterial culture were evaluate...
Granello ME, Young JM, Cleff DB, Banks EBM, Trumble TN.To describe characteristics of postoperative peritoneal fluid following exploratory laparotomy for naturally-occurring gastrointestinal lesions in horses. Methods: Prospective, observational cohort study. Methods: A total of 26 client-owned horses that underwent exploratory laparotomy for naturally-occurring gastrointestinal lesions. Methods: Abdominocentesis was performed pre- or intraoperatively, and at three time points postoperatively (24, 72, and 168 h). Peritoneal lactate, total protein (TP), total nucleated cell count (TNCC), cytology, and systemic lactate were performed at each time ...
Cifuentes V, Zuluaga-Cabrera AM, Vargas-Muñoz LJ, Estrada-Gómez S.Albumin is one of the most abundant and physiologically important blood protein in horses due to its ability to regulate vascular volume and transport blood metabolites or drugs. Despite the importance of this protein, in Colombia there is no previous reference of the use of equine albumin in horses as a pharmacological therapy and there is no available any pharmaceutical preparation of this protein to be administrated in horses. This study aims to evaluate for first time the preliminary clinical response of healthy adult Colombian Creole horses after the intravenous administration of an equin...
Aleman M, Morales CJ.Idiopathic trigeminal-mediated headshaking is a painful neuropathic disorder characterized by sudden, violent, predominantly vertical head movements of unknown etiology. Clinical signs may be seasonal or persistent and triggered by light, sound, exercise, or tactile nasal stimulation. The pathophysiology is multifactorial, involving abnormal trigeminal nerve excitability influenced by hormonal, environmental, and dietary factors. Treatment is challenging and typically multimodal, aiming to reduce trigeminal hypersensitivity and improve quality of life.
Gartland KL, Leśniak K, Twigg-Flesner A.Scientific interest in relation to Fragile Foal Syndrome (FFS) has proliferated in the last decade, but before this, many clinical cases were attributed to other similarly presenting equine neonatal disorders. It was thought that FFS-affected foals were mainly miscarried throughout gestation, but recent study results suggest that often, foals are born alive and die shortly after birth. FFS is proposed to have originated as long ago as the Godolphin Arabian, but the definite derivation of the mutant allele is unconfirmed. The discovery that FFS is present in 11-30% of Warmbloods and 2-3% of Tho...
Mazzucco L, Marcatili M, Withers J, Cantatore F.Superficial digital flexor tendinopathy is a common cause of forelimb lameness in Thoroughbred racehorses and sports horses; however, this condition is rarely observed in the hindlimb. A 16-year-old Irish sports horse gelding was presented with bilateral swelling in the plantar metatarsal region and severe bilateral hindlimb lameness visible at the walk. The lameness occurred after the cross-country phase of an eventing competition. The clinical suspicion of superficial digital flexor tendon (SDFT) injury was confirmed after ultrasonographic examination, which revealed an extensive core lesion...
DeBel NA, Yalavarthi G, Johnson JR, Simoneau AT, Sweeney B, Stearns G, Saliceti A, Rutstein H, Amalaradjou MA, Govoni KE, McBride ZG, Moore TE....Strongyles are nematode parasites that affect the large intestine and cecum of the horse, with symptoms ranging from poor hair coat to colic. This can influence gut health, nutrient absorption, and overall body condition. Objective: Determine the effects of parasitic load, breed, age, housing, and season on skin and hair coat conditions. We hypothesized that horses with low fecal egg counts would have better hair and skin coat condition compared with those with high fecal egg counts. Methods: A total of forty-five Morgan and 11 Thoroughbred horses were included in this study. We tested the eff...
Tsopp E, Kilk K, Gambini A, Tvrdà E, Viljaste-Seera A, Tuppits U, Jaakma Ü.Equine in vitro embryo production (IVP) via ovum pick-up (OPU) and intracytoplasmic sperm injection (ICSI) has gained prominence in sport-horse breeding due to high success rates, efficient semen utilization, and year-round embryo production, including from mares with fertility challenges or postmortem. However, assessing viability of early-stage ICSI-produced equine embryos remains challenging due to subjective morphological evaluations and limited visibility of developmental features. Non-invasive metabolomic analysis of culture media offers a promising alternative to enhance embryo selectio...
Dundjerovic I, Sneddon LU.Animal welfare concerns both the physical and mental wellbeing of animals so the assessment of animals kept in captivity, for example zoos, is important and necessary both legally and ethically. Good welfare is especially vital when breeding endangered species as stress impairs reproductive investment. As zoos often have high numbers of visitors, it is important to know if humans and the associated noise might influence the animals' welfare. The aim of this study was to investigate whether the presence of visitors and activities offered to the visitors caused a stress response in horses housed...
Nixon W, Buckley P, Butler R.There is a lack of information on horse parasite management practices currently used by individual horse owners in Australia. A voluntary, anonymous online survey was developed for and distributed by Pony Club Australia to its members seeking information on horse population; housing; paddock, manure and parasite management; and owner attitudes. From a total of 98 completed responses, 95/98 of participants indicated their horses spent time in paddocks, 91/98 administered anthelmintics, 42/98 guessed their horse's body weight for dosing, 78/98 recalled the dewormer brand or active ingredient las...
Byrne J, Guedes AG, Hickey C, Hatschbach E, Walters B, Rendahl A, Tearney C.To evaluate the utility of the dynamic indices, systolic pressure variation (SPV%), pulse pressure variation (PPV%) and plethysmographic variability index (PVI%) during induced hypovolemia, and to determine their correlation with cardiac output (CO) in anesthetized horses. Methods: Prospective experimental study. Methods: A group of eight healthy adult research horses. Methods: After sedation with intravenous (IV) xylazine, anesthesia was induced with IV ketamine and diazepam and maintained with constant-dose isoflurane delivered in 100% oxygen. Horses were positioned in dorsal recumbency, par...
O'Reilly K, Aldworth-Yang S, Catalano DN.There are many different concentrate feeders available to horse owners, but little data exists on feeder effect of equine intake rate. Rapid consumption ("bolting") is associated with issues such as esophageal obstruction. Objective: The objective of this study was to evaluate three common concentrate feeders on intake rate. We hypothesized horses would have the slowest intake rate when fed from the trough. Methods: Four geldings were fed 0.454 kg of concentrate from a pan on the ground (P), shallow trough (T), or hanging feeder (H). Horses were randomly assigned a first feeder and then rotate...
Boeger L, Volkmann N, Probst J, Kemper N, Venner M.The emergence of antimicrobial resistance raises concerns about using antibiotics in veterinary medicine, particularly in cases of bronchopneumonia in foals diagnosed early based on a screening programme. Many subclinical foals recover spontaneously, thus, interest in more restrictive antibiotic usage is increasing. Objective: To determine whether initiating antimicrobial therapy at a later stage of bronchopneumonia decreases antimicrobial usage without compromising successful resolution of pathology in affected foals. Methods: A total of 1200 warmblood foals born in 2020, 2022, 2023 and 2024 ...
Samuels AN, Collins N, Albrecht M, Cuming R, Reed S, Gomez D, Toribio R.To evaluate associations between the neutrophil-to-lymphocyte ratio (NLR), nonsurvival, illness severity, and infectious etiologies. Unassigned: This was a retrospective multicenter study that included 100 healthy horses and 444 horses presenting with acute diarrhea to 5 equine referral hospitals. Inclusion criteria were diarrhea duration < 48 hours, complete hemograms, and outcome data. Illness severity was assessed using systemic inflammatory response syndrome. The results of pathogen testing for Salmonella spp, equine coronavirus, Clostridium spp, and Neorickettsia risticii/findlayensis ...
Aliani C, Lorenzetto Bologna C, Francia P, Bocchi L.Standing equine computed tomography (CT) acquisitions are susceptible to residual postural sway, which can introduce view-inconsistent motion and degrade image quality. External optical tracking based on ChArUco fiducials is a promising, low-cost strategy to enable projection-wise motion compensation, yet quantitative guidance on how camera-marker geometry affects pose-estimation performance remains limited. This CT-motivated benchtop study characterizes how the relative camera-ChArUco configuration influences both the accuracy (bias with respect to ground truth) and the precision (repeatabili...
Mukai K, Takahashi Y, Ebisuda Y, Sugiyama F, Yoshida T, Miyata H.This study tested the hypothesis that 6 weeks of high-intensity interval training (HIIT) would induce greater physiological adaptations than moderate-intensity continuous training (MICT) in Thoroughbred horses. Seven untrained horses completed two distance-matched treadmill training protocols (three sessions per week) in a randomized crossover design, separated by a three-month washout: MICT (6 min at 70% ) and HIIT (6 × [30 s at 100% with 30 s at 30% ]). Incremental exercise tests were conducted at weeks 0, 3, and 6 to assess exercise performance and physiological responses. M...
Mählmann K, Lischer CJ.This technical note describes the use of a manual graphic roadmap (MGR) to assist fluoroscopic guidance during transarterial coil embolization (TACE) for guttural pouch (GP) arterial hemorrhage. Horses underwent MGR-assisted TACE at the Equine Clinic, Free University of Berlin, between 2012 and 2024. Surgery was performed under general anesthesia with fluoroscopic guidance. Following the initial angiogram, a transparent foil was secured to the fluoroscopy monitor, and an assistant traced the vascular anatomy with a waterproof marker to create the MGR, which then served as a guide for catheter ...
Aimonetti E, Genton M, Lischer CJ, Rossignol F.To determine the benefit of using a three-dimensional (3D)-printed titanium aiming device for placing two 5.5-mm transarticular screws during proximal interphalangeal joint (PIPJ) arthrodesis in equine species. Methods: Ex vivo study. Methods: A total of 40 cadaveric forelimbs from adult horses. Methods: A senior surgeon and a first-year resident each performed 20 constructs: 10 using the device and 10 using a freehand technique. Procedure duration and number of intraoperative radiographs were recorded. Final radiographs were evaluated by an ECVS-certified surgeon unaware of treatment group, b...
Strugava L, Serighelli-Júnior G, Stadler LM, Flores LADS, Minozzo GA, Dornbusch LPTC, Moreno JCD, Dornbusch PT.Rectal amputation is a surgical approach indicated for the management of severe rectal lacerations or extensive rectal prolapse when transmural compromise or primary repair is unfeasible. These conditions, although uncommon, present substantial challenges in equine practice due to the anatomical complexity of the pelvic region and the potential for life-threatening complications if not adequately addressed. This study aimed to describe and standardize a reproducible surgical technique for rectal amputation in horse cadavers. Six equine cadavers (mean body mass, 441.83± 66.84 kg) were position...
Cerullo A, Scilimati N, Di Nicola MR, Colla L, Mazza S, Bertoletti A, Nannarone S, Gialletti R.Left dorsal displacement of the large colon (LDDLC) is a common cause of colic in horses and it is frequently associated with recurrent episodes, with significant implications for horse management, athletic performance, and owner satisfaction. Laparoscopic nephrosplenic space (NSS) ablation is a preventive surgical technique aimed at reducing recurrence in horses with a history of medically or surgically treated LDDLC. This retrospective study evaluated 48 horses that underwent laparoscopic NSS ablation in two Italian Veterinary Teaching Hospitals between 2016 and 2024. Short-term outcomes, in...
Burns JJ, MacMillan KM.The Standardbred racing industry plays an important role in the North American economy and is expected to continue operating and growing. However, public perception is often unfavorable, with increasing concern about the health and welfare of Standardbred racehorses. Despite this, limited research has examined Standardbred well-being. To address this gap, a survey of equine trainers on Prince Edward Island (PEI) was conducted to evaluate key health and welfare issues during racing careers. Results showed that over one quarter of respondents had at least one horse displaying stereotypic behavio...
Zimmerman-Cameron SR, Colbath AC, Caruso MA, Hoffman RM, Logan AA.Navicular syndrome is characterized by palmar foot pain and is a common cause of lameness in horses. Palmar digital neurectomy (nerving) removes sensation to the heel region of the hoof and may serve as a treatment for navicular-afflicted horses, while analgesia (blocking) results in temporary loss of sensation. Objective: The purpose of this pilot study was to examine the effect of loss of sensation in the heel on the forelimb area and force while tracking on soft and hard ground at the walk and trot. Methods: Three horses that scored a 0 or 1 on the AAEP lameness scale were fitted to Tekscan...
Frisbie DD, Cross MW, McIlwraith CW.Histological measurements of the thickness of non-calcified and calcified cartilage, as well as the subchondral bone plate in five locations on the femoral trochlea and medial femoral condyles of species were used in preclinical studies of articular cartilage and compared to those of the human knee. Cadaver specimens were obtained of six human knees, as well as six equine, six goat, six dog, six sheep and six rabbit stifle joints (the animal equivalent of the human knee). Specimens were taken from the lateral trochlear ridge, medial trochlear ridge and medial femoral condyle. After histopathol...
Rosengren Pielberg G, Golovko A, Sundström E, Curik I, Lennartsson J, Seltenhammer MH, Druml T, Binns M, Fitzsimmons C, Lindgren G, Sandberg K....In horses, graying with age is an autosomal dominant trait associated with a high incidence of melanoma and vitiligo-like depigmentation. Here we show that the Gray phenotype is caused by a 4.6-kb duplication in intron 6 of STX17 (syntaxin-17) that constitutes a cis-acting regulatory mutation. Both STX17 and the neighboring NR4A3 gene are overexpressed in melanomas from Gray horses. Gray horses carrying a loss-of-function mutation in ASIP (agouti signaling protein) had a higher incidence of melanoma, implying that increased melanocortin-1 receptor signaling promotes melanoma development in Gra...
Marasas WF, Kellerman TS, Gelderblom WC, Coetzer JA, Thiel PG, van der Lugt JJ.Each of two horses was dosed by stomach tube with culture material on maize of Fusarium moniliforme MRC 826. One horse developed severe hepatosis and mild oedema of the brain after 6 doses of 2.5 g of culture material/kg body mass/day in 7 days. The second horse, in a similar experiment but at a dosage rate of 1.25 g/kg/day, developed mild hepatosis and moderate oedema of the brain. In both animals the brain oedema was particularly noticeable in the medulla oblongata. The mycotoxin fumonisin B1 was extracted and purified from the culture material of F. moniliforme MRC, 826 which contained appr...
Kalbfleisch TS, Rice ES, DePriest MS, Walenz BP, Hestand MS, Vermeesch JR, O Connell BL, Fiddes IT, Vershinina AO, Saremi NF, Petersen JL, Finno CJ....Recent advances in genomic sequencing technology and computational assembly methods have allowed scientists to improve reference genome assemblies in terms of contiguity and composition. EquCab2, a reference genome for the domestic horse, was released in 2007. Although of equal or better quality compared to other first-generation Sanger assemblies, it had many of the shortcomings common to them. In 2014, the equine genomics research community began a project to improve the reference sequence for the horse, building upon the solid foundation of EquCab2 and incorporating new short-read data, lon...
Chaucheyras-Durand F, Durand H.The use of probiotics for farm animals has increased considerably over the last 15 years. Probiotics are defined as live microorganisms which can confer a health benefit for the host when administered in appropriate and regular quantities. Once ingested, the probiotic microorganisms can modulate the balance and activities of the gastrointestinal microbiota, whose role is fundamental to gut homeostasis. It has been demonstrated that numerous factors, such as dietary and management constraints, can strongly affect the structure and activities of the gut microbial communities, leading to impaired...
Hiemstra PS, Maassen RJ, Stolk J, Heinzel-Wieland R, Steffens GJ, Dijkman JH.Antileukoprotease (ALP), or secretory leukocyte proteinase inhibitor, is an endogenous inhibitor of serine proteinases that is present in various external secretions. ALP, one of the major inhibitors of serine proteinases present in the human lung, is a potent reversible inhibitor of elastase and, to a lesser extent, of cathepsin G. In equine neutrophils, an antimicrobial polypeptide that has some of the characteristics of ALP has been identified (M. A. Couto, S. S. L. Harwig, J. S. Cullor, J. P. Hughes, and R. I. Lehrer, Infect. Immun. 60:5042-5047, 1992). This report, together with the catio...
Ahern BJ, Parvizi J, Boston R, Schaer TP.Review the literature for single site cartilage defect research and evaluate the respective strengths and weaknesses of different preclinical animal models. Methods: A literature search for animal models evaluating single site cartilage defects was performed. Variables tabulated and analyzed included animal species, age and number, defect depth and diameter and study duration. Cluster analyses were then used to separate animals with only distal femoral defects into similar groups based on defect dimensions. Representative human studies were included allowing comparison of common clinical lesio...
Makvandi-Nejad S, Hoffman GE, Allen JJ, Chu E, Gu E, Chandler AM, Loredo AI, Bellone RR, Mezey JG, Brooks SA, Sutter NB.Horse body size varies greatly due to intense selection within each breed. American Miniatures are less than one meter tall at the withers while Shires and Percherons can exceed two meters. The genetic basis for this variation is not known. We hypothesize that the breed population structure of the horse should simplify efforts to identify genes controlling size. In support of this, here we show with genome-wide association scans (GWAS) that genetic variation at just four loci can explain the great majority of horse size variation. Unlike humans, which are naturally reproducing and possess many...
MacDonald GH, Johnston RE.The initial steps of Venezuelan equine encephalitis virus (VEE) spread from inoculation in the skin to the draining lymph node have been characterized. By using green fluorescent protein and immunocytochemistry, dendritic cells in the draining lymph node were determined to be the primary target of VEE infection in the first 48 h following inoculation. VEE viral replicon particles, which can undergo only one round of infection, identified Langerhans cells to be the initial set of cells infected by VEE directly following inoculation. These cells are resident dendritic cells in the skin, which mi...
Achilli A, Olivieri A, Soares P, Lancioni H, Hooshiar Kashani B, Perego UA, Nergadze SG, Carossa V, Santagostino M, Capomaccio S, Felicetti M....Archaeological and genetic evidence concerning the time and mode of wild horse (Equus ferus) domestication is still debated. High levels of genetic diversity in horse mtDNA have been detected when analyzing the control region; recurrent mutations, however, tend to blur the structure of the phylogenetic tree. Here, we brought the horse mtDNA phylogeny to the highest level of molecular resolution by analyzing 83 mitochondrial genomes from modern horses across Asia, Europe, the Middle East, and the Americas. Our data reveal 18 major haplogroups (A-R) with radiation times that are mostly confined ...
Petersen JL, Mickelson JR, Cothran EG, Andersson LS, Axelsson J, Bailey E, Bannasch D, Binns MM, Borges AS, Brama P, da Câmara Machado A, Distl O....Horses were domesticated from the Eurasian steppes 5,000-6,000 years ago. Since then, the use of horses for transportation, warfare, and agriculture, as well as selection for desired traits and fitness, has resulted in diverse populations distributed across the world, many of which have become or are in the process of becoming formally organized into closed, breeding populations (breeds). This report describes the use of a genome-wide set of autosomal SNPs and 814 horses from 36 breeds to provide the first detailed description of equine breed diversity. F(ST) calculations, parsimony, and dista...
Petersen JL, Mickelson JR, Rendahl AK, Valberg SJ, Andersson LS, Axelsson J, Bailey E, Bannasch D, Binns MM, Borges AS, Brama P, da Câmara Machado A....Intense selective pressures applied over short evolutionary time have resulted in homogeneity within, but substantial variation among, horse breeds. Utilizing this population structure, 744 individuals from 33 breeds, and a 54,000 SNP genotyping array, breed-specific targets of selection were identified using an F(ST)-based statistic calculated in 500-kb windows across the genome. A 5.5-Mb region of ECA18, in which the myostatin (MSTN) gene was centered, contained the highest signature of selection in both the Paint and Quarter Horse. Gene sequencing and histological analysis of gluteal muscle...
Frisbie DD, Ghivizzani SC, Robbins PD, Evans CH, McIlwraith CW.Osteoarthritis in horses and in humans is a significant social and economic problem and continued research and improvements in therapy are needed. Because horses have naturally occurring osteoarthritis, which is similar to that of humans, the horse was chosen as a species with which to investigate gene transfer as a potential therapeutic modality for the clinical treatment of osteoarthritis. Using an established model of equine osteoarthritis that mimics clinical osteoarthritis, the therapeutic effects resulting from intra-articular overexpression of the equine interleukin-1 receptor antagonis...
Saenz RA, Quinlivan M, Elton D, Macrae S, Blunden AS, Mumford JA, Daly JM, Digard P, Cullinane A, Grenfell BT, McCauley JW, Wood JL, Gog JR.A key question in pandemic influenza is the relative roles of innate immunity and target cell depletion in limiting primary infection and modulating pathology. Here, we model these interactions using detailed data from equine influenza virus infection, combining viral and immune (type I interferon) kinetics with estimates of cell depletion. The resulting dynamics indicate a powerful role for innate immunity in controlling the rapid peak in virus shedding. As a corollary, cells are much less depleted than suggested by a model of human influenza based only on virus-shedding data. We then explore...
McCue ME, Bannasch DL, Petersen JL, Gurr J, Bailey E, Binns MM, Distl O, Guérin G, Hasegawa T, Hill EW, Leeb T, Lindgren G, Penedo MC, Røed KH....An equine SNP genotyping array was developed and evaluated on a panel of samples representing 14 domestic horse breeds and 18 evolutionarily related species. More than 54,000 polymorphic SNPs provided an average inter-SNP spacing of ∼43 kb. The mean minor allele frequency across domestic horse breeds was 0.23, and the number of polymorphic SNPs within breeds ranged from 43,287 to 52,085. Genome-wide linkage disequilibrium (LD) in most breeds declined rapidly over the first 50-100 kb and reached background levels within 1-2 Mb. The extent of LD and the level of inbreeding were highest in the ...
Frisbie DD, Oxford JT, Southwood L, Trotter GW, Rodkey WG, Steadman JR, Goodnight JL, McIlwraith CW.The current study investigated healing of large full-thickness articular cartilage defects during the first 8 weeks with and without penetration of the subchondral bone using microfracture in an established equine model of cartilage healing. Chondral defects in the weightbearing portion of the medial femoral condyle were made bilaterally; one defect in each horse was microfractured whereas the contralateral leg served as the control. The expression of cartilage extracellular matrix components (Types I and II collagen and aggrecan) was evaluated using histologic techniques, reverse transcriptio...
Yu L, Wei Y, Sun HX, Mahdi AK, Pinzon Arteaga CA, Sakurai M, Schmitz DA, Zheng C, Ballard ED, Li J, Tanaka N, Kohara A, Okamura D, Mutto AA, Gu Y....Dynamic pluripotent stem cell (PSC) states are in vitro adaptations of pluripotency continuum in vivo. Previous studies have generated a number of PSCs with distinct properties. To date, however, no known PSCs have demonstrated dual competency for chimera formation and direct responsiveness to primordial germ cell (PGC) specification, a unique functional feature of formative pluripotency. Here, by modulating fibroblast growth factor (FGF), transforming growth factor β (TGF-β), and WNT pathways, we derived PSCs from mice, horses, and humans (designated as XPSCs) that are permissive for dire...
Science (New York, N.Y.)February 13, 2001
Volume 291, Issue 5503 474-477 doi: 10.1126/science.291.5503.474
Vilà C, Leonard JA, Gotherstrom A, Marklund S, Sandberg K, Liden K, Wayne RK, Ellegren H.Domestication entails control of wild species and is generally regarded as a complex process confined to a restricted area and culture. Previous DNA sequence analyses of several domestic species have suggested only a limited number of origination events. We analyzed mitochondrial DNA (mtDNA) control region sequences of 191 domestic horses and found a high diversity of matrilines. Sequence analysis of equids from archaeological sites and late Pleistocene deposits showed that this diversity was not due to an accelerated mutation rate or an ancient domestication event. Consequently, high mtDNA se...
Lunn DP, Davis-Poynter N, Flaminio MJ, Horohov DW, Osterrieder K, Pusterla N, Townsend HG.Equine herpesvirus-1 is a highly prevalent and frequently pathogenic infection of equids. The most serious clinical consequences of infection are abortion and equine herpesvirus myeloencephalopathy (EHM). In recent years, there has been an apparent increase in the incidence of EHM in North America, with serious consequences for horses and the horse industry. This consensus statement draws together current knowledge in the areas of pathogenesis, strain variation, epidemiology, diagnostic testing, vaccination, outbreak prevention and control, and treatment.
O'Sullivan JD, Allworth AM, Paterson DL, Snow TM, Boots R, Gleeson LJ, Gould AR, Hyatt AD, Bradfield J.In September, 1994, an outbreak of severe respiratory disease affected 18 horses, their trainer, and a stablehand in Queensland, Australia. Fourteen horses and one human being died. A novel virus was isolated from those affected and named equine morbillivirus (EMV). We report a case of encephalitis caused by this virus. Results: A 35-year-old man from Queensland had a brief aseptic meningitic illness in August, 1994, shortly after caring for two horses that died from EMV infection and then assisting at their necropsies. He then suffered severe encephalitis 13 months later, characterised by unc...
Costa MC, Silva G, Ramos RV, Staempfli HR, Arroyo LG, Kim P, Weese JS.The advance of new sequencing technologies has allowed more comprehensive characterization of complex microbial communities, including the ones inhabiting the intestinal tract. The presence of extreme environmental filters, such as low pH, digestive enzymes and anaerobic conditions along the tract, acts on the selection of unique bacteria in each compartment. The intestinal microbiota has an enormous impact on the maintenance of health. However, data about the bacteria present in the different intestinal compartments of horses are sparse. In this study, high throughput sequencing was used to c...
Williamson MM, Hooper PT, Selleck PW, Gleeson LJ, Daniels PW, Westbury HA, Murray PK.To determine the infectivity and transmissibility of Hendra virus (HeV). Methods: A disease transmission study using fruit bats, horses and cats. Methods: Eight grey-headed fruit bats (Pteropus poliocephalus) were inoculated and housed in contact with three uninfected bats and two uninfected horses. In a second experiment, four horses were inoculated by subcutaneous injection and intranasal inoculation and housed in contact with three uninfected horses and six uninfected cats. In a third experiment, 12 cats were inoculated and housed in contact with three uninfected horses. Two surviving horse...
Gleerup KB, Forkman B, Lindegaard C, Andersen PH.The objective of this study was to investigate the existence of an equine pain face and to describe this in detail. Methods: Semi-randomized, controlled, crossover trial. Methods: Six adult horses. Methods: Pain was induced with two noxious stimuli, a tourniquet on the antebrachium and topical application of capsaicin. All horses participated in two control trials and received both noxious stimuli twice, once with and once without an observer present. During all sessions their pain state was scored. The horses were filmed and the close-up video recordings of the faces were analysed for alterat...
Frisbie DD, Kisiday JD, Kawcak CE, Werpy NM, McIlwraith CW.The purpose of this study was the assessment of clinical, biochemical, and histologic effects of intraarticular administered adipose-derived stromal vascular fraction or bone marrow-derived mesenchymal stem cells for treatment of osteoarthritis. Osteoarthritis was induced arthroscopically in the middle carpal joint of all horses, the contralateral joint being sham-operated. All horses received treatment on Day 14. Eight horses received placebo treatment and eight horses received adipose-derived stromal vascular fraction in their osteoarthritis-affected joint. The final eight horses were treate...